Tan0003490 (gene) Snake gourd v1

Overview
NameTan0003490
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionE3 ubiquitin-protein ligase At1g63170-like
LocationLG05: 34147543 .. 34148238 (-)
RNA-Seq ExpressionTan0003490
SyntenyTan0003490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTTCGCAAACACCTCCACCCTCCGCTACTCTCTCCGCCGCAGCGACGGCGCTGATGATCAACTCCTCCAAGTCCGATTGGGGTATTCCAGCCGTTTGATCTCCCGATCGCCGTTCGTCGGACTTCCGTACGATATCCTCCAAGAAACTCCGTCGACTCCAATGGCGGATGCTCTGTTCCCTCTGCCTCTACGAGAGCTCGAAGATCCGTTACTATGCCAGGCTTACGTGGTTGAATTGCTGTCCTTCTTCAACCTCCATCCCCTCAGCTGCAACCCCATTGGTCGGAAGGTCGCGTCGTTCGTGGTTCAGTTGGCCGCCGGAGACTACGACACGAAGTTCCAGTTGGTCGCTGAAATTGACTACATCCAAATGTTTTGGATGGAAGAGCAGTGGAGTTCTGTTCGTGTGGAGAATAATGTTGGGCGTGATCGTGTAATGGTGGAGGTTGAGGAGAGGGTGGTTCTCGACGAAGCTCCACCGCCGCCGGCGAAGCATAAATTCGAGGTATTTAGGCAAGAAGAGCTGGGAGAATGCAGTGTGTGTTACGAATCTTACGATGAATTGAACTTGAAGGGAGGCGAGAAGGAGGTGGCCAGAATCCCATGCGGTCATGTCTATCACAAATCCTGCATTCTCACCTGGTTTCAAAGGAGCAACACATGTCCTTTGTGTAGAAGGAAATTAAATTAG

mRNA sequence

ATGCCTTTCGCAAACACCTCCACCCTCCGCTACTCTCTCCGCCGCAGCGACGGCGCTGATGATCAACTCCTCCAAGTCCGATTGGGGTATTCCAGCCGTTTGATCTCCCGATCGCCGTTCGTCGGACTTCCGTACGATATCCTCCAAGAAACTCCGTCGACTCCAATGGCGGATGCTCTGTTCCCTCTGCCTCTACGAGAGCTCGAAGATCCGTTACTATGCCAGGCTTACGTGGTTGAATTGCTGTCCTTCTTCAACCTCCATCCCCTCAGCTGCAACCCCATTGGTCGGAAGGTCGCGTCGTTCGTGGTTCAGTTGGCCGCCGGAGACTACGACACGAAGTTCCAGTTGGTCGCTGAAATTGACTACATCCAAATGTTTTGGATGGAAGAGCAGTGGAGTTCTGTTCGTGTGGAGAATAATGTTGGGCGTGATCGTGTAATGGTGGAGGTTGAGGAGAGGGTGGTTCTCGACGAAGCTCCACCGCCGCCGGCGAAGCATAAATTCGAGGTATTTAGGCAAGAAGAGCTGGGAGAATGCAGTGTGTGTTACGAATCTTACGATGAATTGAACTTGAAGGGAGGCGAGAAGGAGGTGGCCAGAATCCCATGCGGTCATGTCTATCACAAATCCTGCATTCTCACCTGGTTTCAAAGGAGCAACACATGTCCTTTGTGTAGAAGGAAATTAAATTAG

Coding sequence (CDS)

ATGCCTTTCGCAAACACCTCCACCCTCCGCTACTCTCTCCGCCGCAGCGACGGCGCTGATGATCAACTCCTCCAAGTCCGATTGGGGTATTCCAGCCGTTTGATCTCCCGATCGCCGTTCGTCGGACTTCCGTACGATATCCTCCAAGAAACTCCGTCGACTCCAATGGCGGATGCTCTGTTCCCTCTGCCTCTACGAGAGCTCGAAGATCCGTTACTATGCCAGGCTTACGTGGTTGAATTGCTGTCCTTCTTCAACCTCCATCCCCTCAGCTGCAACCCCATTGGTCGGAAGGTCGCGTCGTTCGTGGTTCAGTTGGCCGCCGGAGACTACGACACGAAGTTCCAGTTGGTCGCTGAAATTGACTACATCCAAATGTTTTGGATGGAAGAGCAGTGGAGTTCTGTTCGTGTGGAGAATAATGTTGGGCGTGATCGTGTAATGGTGGAGGTTGAGGAGAGGGTGGTTCTCGACGAAGCTCCACCGCCGCCGGCGAAGCATAAATTCGAGGTATTTAGGCAAGAAGAGCTGGGAGAATGCAGTGTGTGTTACGAATCTTACGATGAATTGAACTTGAAGGGAGGCGAGAAGGAGGTGGCCAGAATCCCATGCGGTCATGTCTATCACAAATCCTGCATTCTCACCTGGTTTCAAAGGAGCAACACATGTCCTTTGTGTAGAAGGAAATTAAATTAG

Protein sequence

MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADALFPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAEIDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELGECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKLN
Homology
BLAST of Tan0003490 vs. ExPASy Swiss-Prot
Match: Q9Y4L5 (E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.1e-09
Identity = 31/79 (39.24%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 161 PPPPAKHKFEVF-------RQEELG-ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSC 220
           PPP  K K            Q ++G EC VC E Y        E+EV ++PC H +H SC
Sbjct: 201 PPPADKEKITSLPTVTVTQEQVDMGLECPVCKEDYTV------EEEVRQLPCNHFFHSSC 260

Query: 221 ILTWFQRSNTCPLCRRKLN 232
           I+ W +  +TCP+CR+ LN
Sbjct: 261 IVPWLELHDTCPVCRKSLN 273

BLAST of Tan0003490 vs. ExPASy Swiss-Prot
Match: Q9D0C1 (E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.0e-09
Identity = 30/79 (37.97%), Postives = 42/79 (53.16%), Query Frame = 0

Query: 161 PPPPAKHKFE-----VFRQEELG---ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSC 220
           PPP  K K          QE++    EC VC E Y        E++V ++PC H +H SC
Sbjct: 202 PPPADKEKITSLPTVTVTQEQVNTGLECPVCKEDYTV------EEKVRQLPCNHFFHSSC 261

Query: 221 ILTWFQRSNTCPLCRRKLN 232
           I+ W +  +TCP+CR+ LN
Sbjct: 262 IVPWLELHDTCPVCRKSLN 274

BLAST of Tan0003490 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.1e-08
Identity = 29/76 (38.16%), Postives = 42/76 (55.26%), Query Frame = 0

Query: 161 PPPPAKHKFE-----VFRQEELG-ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCIL 220
           PPP AK   E     V R  +   +C VC   ++E      E+ V  +PC H++H +CIL
Sbjct: 63  PPPAAKAVVESLPRTVIRSSKAELKCPVCLLEFEE------EETVIEMPCHHLFHSNCIL 122

Query: 221 TWFQRSNTCPLCRRKL 231
            W  ++N+CPLCR +L
Sbjct: 123 PWLSKTNSCPLCRHEL 132

BLAST of Tan0003490 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.6e-08
Identity = 31/77 (40.26%), Postives = 43/77 (55.84%), Query Frame = 0

Query: 161 PPPPAKHKFE-----VFR--QEELGECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCI 220
           PPP AK   E     V R  Q EL +C VC   ++E      E+    +PC H++H +CI
Sbjct: 51  PPPAAKTAVENLPRTVIRGSQAEL-KCPVCLLEFEE------EETAIEMPCHHLFHSNCI 110

Query: 221 LTWFQRSNTCPLCRRKL 231
           L W  ++N+CPLCR +L
Sbjct: 111 LPWLSKTNSCPLCRHEL 120

BLAST of Tan0003490 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.6e-08
Identity = 32/77 (41.56%), Postives = 43/77 (55.84%), Query Frame = 0

Query: 161 PPPPAKHKFE-----VFR--QEELGECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCI 220
           PPP AK   E     V R  Q EL +C VC   ++E      E+    +PC H++H SCI
Sbjct: 51  PPPAAKTVVENLPRTVIRGSQAEL-KCPVCLLEFEE------EETAIEMPCHHLFHSSCI 110

Query: 221 LTWFQRSNTCPLCRRKL 231
           L W  ++N+CPLCR +L
Sbjct: 111 LPWLSKTNSCPLCRYEL 120

BLAST of Tan0003490 vs. NCBI nr
Match: KAG6605222.1 (E3 ubiquitin-protein ligase AMFR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 298.5 bits (763), Expect = 4.9e-77
Identity = 160/232 (68.97%), Postives = 181/232 (78.02%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           MPFAN STLRYSLRR+ GA DQLLQ+RLGYSSRLISRSP  GLPYDIL+ETPSTPMADAL
Sbjct: 1   MPFANASTLRYSLRRNHGA-DQLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S FNL  ++ NPI +K+ SFV Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSFNLGSVTWNPIVQKITSFVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-- 180
           IDYIQMFWME++  S      VGR    V VEERVVL+E     A+       ++ +G  
Sbjct: 121 IDYIQMFWMEKRGGS------VGRG---VMVEERVVLEETGVAAAE-------KQGIGGE 180

Query: 181 ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           ECSVCYESY+    KGGEKEVA+IPCGH +HKSCILTWFQRSN+CPLCR KL
Sbjct: 181 ECSVCYESYEA--EKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Tan0003490 vs. NCBI nr
Match: XP_022947841.1 (NEP1-interacting protein-like 1 [Cucurbita moschata])

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-76
Identity = 159/232 (68.53%), Postives = 180/232 (77.59%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           MPFAN STLRYSLRR+ GA DQLLQ+RLGYSSRLISRSP  GLPYDIL+ETPSTPMADAL
Sbjct: 1   MPFANASTLRYSLRRNHGA-DQLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S FNL  ++ NPI +K+ SFV Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSFNLGSVTWNPIVQKITSFVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-- 180
           IDYIQMFWME+         +VGR    V VEERVVL+E     A+       ++ +G  
Sbjct: 121 IDYIQMFWMEKH------RGSVGRG---VMVEERVVLEETGVAAAE-------KQGIGGE 180

Query: 181 ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           ECSVCYESY+    KGGEKEVA+IPCGH +HKSCILTWFQRSN+CPLCR KL
Sbjct: 181 ECSVCYESYEA--EKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Tan0003490 vs. NCBI nr
Match: XP_023006825.1 (NEP1-interacting protein-like 1 [Cucurbita maxima])

HSP 1 Score: 290.0 bits (741), Expect = 1.8e-74
Identity = 156/234 (66.67%), Postives = 176/234 (75.21%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           MP AN STLRYSLRR+ GA DQ L+ RL YSSRLISRSP  GLPYDIL+ETPSTPMADAL
Sbjct: 1   MPLANASTLRYSLRRNHGA-DQFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY++EL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLIELVSSLNLDSMSCNPIVQKITSLVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-- 180
           IDYIQMFWME++        +VGR    V VEE+VVL EA         EV   E+ G  
Sbjct: 121 IDYIQMFWMEKR------PGSVGRG---VMVEEKVVLKEA---------EVAAAEKQGIG 180

Query: 181 --ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
             ECSVCYESY+    KGGE+EVA+IPCGHV+HKSCILTWFQRSN+CPLCR KL
Sbjct: 181 GEECSVCYESYEA--EKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Tan0003490 vs. NCBI nr
Match: XP_023534304.1 (uncharacterized protein LOC111795903 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.5 bits (737), Expect = 5.1e-74
Identity = 157/231 (67.97%), Postives = 173/231 (74.89%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           M FAN+STLRYSLRR DG  DQLLQVRLGYSSRLISRSP+ GL YDIL+ETPST +ADAL
Sbjct: 1   MLFANSSTLRYSLRRKDGT-DQLLQVRLGYSSRLISRSPYSGLSYDILEETPSTLIADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSLNLDSISCNPIAQKITSLVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-E 180
           IDYIQMFW EE  S VR           V VEERVVL+EA            +Q   G E
Sbjct: 121 IDYIQMFWREESRSPVRRG---------VMVEERVVLEEAGVAATT---AAGKQGMAGEE 180

Query: 181 CSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           CSVCYE YDE   KGG+KEVARIPCGH++HKSCILTWFQ SN+CPLCR KL
Sbjct: 181 CSVCYECYDE--EKGGDKEVARIPCGHMFHKSCILTWFQCSNSCPLCRAKL 216

BLAST of Tan0003490 vs. NCBI nr
Match: KAG6605180.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.0 bits (733), Expect = 1.5e-73
Identity = 152/230 (66.09%), Postives = 171/230 (74.35%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           M FAN+STLRYSLRR DG  DQLLQVRLGYSSRLISRSP+ GLPYDIL+E PSTP+AD L
Sbjct: 1   MLFANSSTLRYSLRRKDGT-DQLLQVRLGYSSRLISRSPYSGLPYDILEEIPSTPIADTL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSLNLDSISCNPIAQKITSLVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELGEC 180
           IDYIQMFW EE    VR           V VEE+V+L+EA         +     E  EC
Sbjct: 121 IDYIQMFWREESRGPVRRG---------VMVEEKVILEEAGVAATSAAGKQGMGGE--EC 180

Query: 181 SVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           SVCYE YDE   KGG+KEVA+IPCGH++HKSCILTWFQ SN+CPLCR KL
Sbjct: 181 SVCYECYDE--EKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 216

BLAST of Tan0003490 vs. ExPASy TrEMBL
Match: A0A6J1G7L1 (NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 5.3e-77
Identity = 159/232 (68.53%), Postives = 180/232 (77.59%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           MPFAN STLRYSLRR+ GA DQLLQ+RLGYSSRLISRSP  GLPYDIL+ETPSTPMADAL
Sbjct: 1   MPFANASTLRYSLRRNHGA-DQLLQLRLGYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S FNL  ++ NPI +K+ SFV Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSFNLGSVTWNPIVQKITSFVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-- 180
           IDYIQMFWME+         +VGR    V VEERVVL+E     A+       ++ +G  
Sbjct: 121 IDYIQMFWMEKH------RGSVGRG---VMVEERVVLEETGVAAAE-------KQGIGGE 180

Query: 181 ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           ECSVCYESY+    KGGEKEVA+IPCGH +HKSCILTWFQRSN+CPLCR KL
Sbjct: 181 ECSVCYESYEA--EKGGEKEVAKIPCGHAFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Tan0003490 vs. ExPASy TrEMBL
Match: A0A6J1L186 (NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.5e-75
Identity = 156/234 (66.67%), Postives = 176/234 (75.21%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           MP AN STLRYSLRR+ GA DQ L+ RL YSSRLISRSP  GLPYDIL+ETPSTPMADAL
Sbjct: 1   MPLANASTLRYSLRRNHGA-DQFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY++EL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLIELVSSLNLDSMSCNPIVQKITSLVTQWAAVDYDAKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-- 180
           IDYIQMFWME++        +VGR    V VEE+VVL EA         EV   E+ G  
Sbjct: 121 IDYIQMFWMEKR------PGSVGRG---VMVEEKVVLKEA---------EVAAAEKQGIG 180

Query: 181 --ECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
             ECSVCYESY+    KGGE+EVA+IPCGHV+HKSCILTWFQRSN+CPLCR KL
Sbjct: 181 GEECSVCYESYEA--EKGGEREVAKIPCGHVFHKSCILTWFQRSNSCPLCRAKL 213

BLAST of Tan0003490 vs. ExPASy TrEMBL
Match: A0A6J1L191 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111499501 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.0e-72
Identity = 152/231 (65.80%), Postives = 170/231 (73.59%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           M FAN+STLRYSLRR  G  DQLLQVRLGYSSRLIS+SP  GLPYDIL+ETP TP+ADAL
Sbjct: 1   MLFANSSTLRYSLRRHHGT-DQLLQVRLGYSSRLISQSPSAGLPYDILEETPLTPIADAL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLV +
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSLNLDSMSCNPIIQKITSLVTQWAAVDYDAKFQLVVD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELG-E 180
           IDYIQMFW EE    VR           V VEER+VLD+A            +Q   G E
Sbjct: 121 IDYIQMFWREESRGPVRRG---------VMVEERIVLDKAGVAATVAAAAAGKQGMGGEE 180

Query: 181 CSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           CSVCYE YDE   KGG+KEVA+IPCGH++HKSCILTWFQ SN+CPLCR KL
Sbjct: 181 CSVCYECYDE--EKGGDKEVAKIPCGHMFHKSCILTWFQCSNSCPLCRAKL 219

BLAST of Tan0003490 vs. ExPASy TrEMBL
Match: A0A6J1G7R8 (E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451612 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.4e-72
Identity = 152/230 (66.09%), Postives = 170/230 (73.91%), Query Frame = 0

Query: 1   MPFANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVGLPYDILQETPSTPMADAL 60
           M FAN+STLRYSLRR DG  DQLLQVRLGYSSRLISRSP+ GLPYDIL+ETPST +AD L
Sbjct: 1   MLFANSSTLRYSLRRKDGT-DQLLQVRLGYSSRLISRSPYSGLPYDILEETPSTLIADTL 60

Query: 61  FPLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQLAAGDYDTKFQLVAE 120
           FPLPLRELEDPL C AY+VEL+S  NL  +SCNPI +K+ S V Q AA DYD KFQLVA+
Sbjct: 61  FPLPLRELEDPLFCHAYLVELVSSLNLDSISCNPIAQKITSLVTQWAAVDYDLKFQLVAD 120

Query: 121 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQEELGEC 180
           IDYIQMFW EE    VR           V VEE+VVL+EA         +     E  EC
Sbjct: 121 IDYIQMFWREESRGPVRRG---------VMVEEKVVLEEAGVAATSAAGKQGMGGE--EC 180

Query: 181 SVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           SVCYE YDE   KGG+KEVA+IPCGH++HKSCILTWF  SN+CPLCR KL
Sbjct: 181 SVCYECYDE--EKGGDKEVAKIPCGHMFHKSCILTWFLCSNSCPLCRAKL 216

BLAST of Tan0003490 vs. ExPASy TrEMBL
Match: A0A5D3BYE9 (RING finger protein 165-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00110 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 2.0e-39
Identity = 92/231 (39.83%), Postives = 138/231 (59.74%), Query Frame = 0

Query: 3   FANTSTLRYSLRRSDGADDQLLQVRLGYSSRLISRSPFVG-LPYDILQETPSTPMADALF 62
           F + S  RY++RR D ++DQ L ++LG   + +SR P+ G   ++IL E+ + P+   +F
Sbjct: 5   FQSDSISRYTVRRCDDSNDQSLLIQLGIVHQQLSRPPYHGPTNHNILHESNTVPLRITVF 64

Query: 63  PLPLRELEDPLLCQAYVVELLSFFNLHPLSCNPIGRKVASFVVQ-LAAGDYDTKFQLVAE 122
           PLPL+EL+DP +C  Y   LL++ +L   +C+ + + + S + + LAAG+ + KF++VA 
Sbjct: 65  PLPLQELQDPSICHLYTQRLLAYVDLDIETCDIVAKDITSLIGEVLAAGNLEVKFRIVAL 124

Query: 123 IDYIQMFWMEEQWSSVRVENNVGRDRVMVEVEERVVLDEAPPPPAKHKFEVFRQ-EELGE 182
           ID +QM W EE   S         +R+M+                  KF+ F +  ELG+
Sbjct: 125 IDRVQMIWREETGKSRGGSRRAAIERLMM----------------MEKFDGFEEGRELGD 184

Query: 183 CSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           C VCYE      LKG EKEV+RI CGHVYHKSCI+ W  R+++CPLCR K+
Sbjct: 185 CCVCYE-----ELKGEEKEVSRIGCGHVYHKSCIINWVLRNDSCPLCRSKI 214

BLAST of Tan0003490 vs. TAIR 10
Match: AT4G12210.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.5e-10
Identity = 27/57 (47.37%), Postives = 34/57 (59.65%), Query Frame = 0

Query: 176 ELGECSVCYESYDELNLKGGEK--EVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           E   CS+C E     NL  G K  ++ R+ C HV+H  C+L WF R NTCPLCR +L
Sbjct: 149 ETESCSICLE-----NLVSGPKPSDLTRMTCSHVFHNPCLLEWFMRKNTCPLCRTEL 200

BLAST of Tan0003490 vs. TAIR 10
Match: AT4G12190.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.1e-09
Identity = 26/57 (45.61%), Postives = 34/57 (59.65%), Query Frame = 0

Query: 176 ELGECSVCYESYDELNLKGGEK--EVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           E   CS+C ES     L  G K  ++ R+ C HV+H  C+L W +R NTCPLCR +L
Sbjct: 18  ETEPCSICLES-----LVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPLCRTEL 69

BLAST of Tan0003490 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.8e-09
Identity = 26/57 (45.61%), Postives = 34/57 (59.65%), Query Frame = 0

Query: 176 ELGECSVCYESYDELNLKGGEK--EVARIPCGHVYHKSCILTWFQRSNTCPLCRRKL 231
           E   CS+C ES     L  G K  +V R+ C HV+H  C+L W +R NTCPLCR ++
Sbjct: 153 ETEPCSICLES-----LVSGPKPRDVTRMTCSHVFHNGCLLEWLKRKNTCPLCRTEI 204

BLAST of Tan0003490 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.6e-09
Identity = 23/62 (37.10%), Postives = 34/62 (54.84%), Query Frame = 0

Query: 166 KHKFEVFRQEELGECSVCYESYDELNLKGGEKEVARIPCGHVYHKSCILTWFQRSNTCPL 225
           K+K      +E+  C VC E Y E      E+E+ R+ CGH +H  CI  W ++ N CP+
Sbjct: 578 KYKSSTRSSQEVEPCCVCQEEYKE------EEEIGRLECGHDFHSQCIKEWLKQKNLCPI 633

Query: 226 CR 228
           C+
Sbjct: 638 CK 633

BLAST of Tan0003490 vs. TAIR 10
Match: AT2G47560.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 8.0e-09
Identity = 30/68 (44.12%), Postives = 36/68 (52.94%), Query Frame = 0

Query: 161 PPPPAKHKFEVFRQEELGECSVCYESYDELNLKGGEKEVARIP-CGHVYHKSCILTWFQR 220
           PPPP          EE  ECSVC   ++E      E E   +P CGH +H  CI TWF+ 
Sbjct: 99  PPPP----------EEKEECSVCLSEFEE------EDEGRLLPKCGHSFHVDCIDTWFRS 150

Query: 221 SNTCPLCR 228
            +TCPLCR
Sbjct: 159 RSTCPLCR 150

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y4L52.1e-0939.24E3 ubiquitin-protein ligase RNF115 OS=Homo sapiens OX=9606 GN=RNF115 PE=1 SV=2[more]
Q9D0C16.0e-0937.97E3 ubiquitin-protein ligase RNF115 OS=Mus musculus OX=10090 GN=Rnf115 PE=1 SV=1[more]
Q6AXU45.1e-0838.16E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ... [more]
Q3T0W36.6e-0840.26E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1[more]
Q9P0P06.6e-0841.56E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6605222.14.9e-7768.97E3 ubiquitin-protein ligase AMFR, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022947841.11.1e-7668.53NEP1-interacting protein-like 1 [Cucurbita moschata][more]
XP_023006825.11.8e-7466.67NEP1-interacting protein-like 1 [Cucurbita maxima][more]
XP_023534304.15.1e-7467.97uncharacterized protein LOC111795903 [Cucurbita pepo subsp. pepo][more]
KAG6605180.11.5e-7366.09E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1G7L15.3e-7768.53NEP1-interacting protein-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111451601 PE... [more]
A0A6J1L1868.5e-7566.67NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4... [more]
A0A6J1L1911.0e-7265.80E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1G7R81.4e-7266.09E3 ubiquitin-protein ligase MBR2-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
A0A5D3BYE92.0e-3939.83RING finger protein 165-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT4G12210.15.5e-1047.37RING/U-box superfamily protein [more]
AT4G12190.12.1e-0945.61RING/U-box superfamily protein [more]
AT4G05350.12.8e-0945.61RING/U-box superfamily protein [more]
AT1G45180.13.6e-0937.10RING/U-box superfamily protein [more]
AT2G47560.18.0e-0944.12RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 180..226
e-value: 1.2E-8
score: 44.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 179..227
e-value: 2.3E-12
score: 47.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 180..227
score: 13.153163
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 150..231
e-value: 7.5E-18
score: 66.0
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 153..230
NoneNo IPR availablePANTHERPTHR15710:SF137ZINC FINGER, C3HC4 TYPE (RING FINGER) PROTEINcoord: 153..230
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 179..230

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003490.1Tan0003490.1mRNA