Tan0003435 (gene) Snake gourd v1

Overview
NameTan0003435
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPhytocyanin domain-containing protein
LocationLG08: 2358411 .. 2361141 (+)
RNA-Seq ExpressionTan0003435
SyntenyTan0003435
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAGCAAAATGAGTTTTGTTTGGTGTACGATCGCTTTCGCATGTGCAGCCATGGCTGCAACAGCAATGGCTGCAACCACCCATGTAGTTGGAGGTGACGAAGGCTGGAAAATTCCAACCGACGTGTCGTTTTACGCCAAGTGGACTGCCGATAAGACCTTCAATGTTGGTGACAAACTTGGTAAGAAGCTTTTAAACCCTCTCTAATGGCTGTCTTTGTCTTCCTCAAATCTTGCTGCAGCTTCCTAGCAGAGTTTCTTCCTTTCTAACTTCCTCAAGTATTTGTTTTCAACTCTTCTAGTTCTAATCTTGTCAAAGCTTCCTCGAAGCCCTTCGTTCGAGTGGTAAGTGCAATTCTAACTCTATAGTTCAAAAACTCCAAGTGGATGATAGGTTCATGGATCTAAGATAATGAATTGATGAAATTTTAACTTCTCGTCTAACCATTTAGTTTTTTGAAATTTACGCTCTCTCTCCTCATGGATTTTTCTTTTTGTTGTCTATTTTTTATAAATGTTTTCAAAATCCAAGCCAAGTTTAAAAAACTAAAAAGAGTAGTTTTTTAGAAGTTTTTTCTTTTCTAGAATTTGACTAAGAATTCAAATGTGTTTCTTAAGAAAAATGAAACTCATCGTAAACAAAACGCAAGAAAACAAGGTCTCGTTTGATAATCATTTAGGTTGTGATGTTTGGTTTTTGAAAATTGTGATTGTTTTTTTAATATTTCTTTACAATGGTTTCCATCTTTCTTAAGATAAGTATTTGAGTTTCTAGTCAACTTCTAAAAACAAAAATAAGTTTTTGAAAACTATTTTTTTAGACTCCGTTTAATAACCATTTGATTTTTTATTTTAGATTTTTAAAAATTATGTTTGTTTTTTCAAAACTTCTTTCATATTCTCTACAATTAGTTCTATCTTTCTTGAAGTACCATTTAAGATTCTAGCCAAATGTCAAAAACAAAAACAAGTTTTTTAAAACTACTTTTTTTAGTTTTCAAAACTTAGCTTAGTTTTTGAAATCATAGGTATAAAGTACATAAGAAAACATAAAAACTCATTGGTAGATGTAATATTTTTAGGCTTAATTTTCAAAAACCAAATAGTTGCCAAACAGAGGCTTAGTTTTCAAAACTTGGTTTGGATTTTAAAAACATAAATAAAAAGTAGATAAGAAAACATGAAAACTCATGGGTGAAAGTAGTATTTATAGGCTTAATTTTCAAAAACTAAAAACAAGAAATTAAGGTCTCATTTTATAACCATTTAGGCCCGTTCGATAACCATTTTGTTTTTTGTTTTTAGCTTTTGAAATTTAAACTTAAAAATGCTACTTCTATCAATGAGTTTATATGTCATTTTATATACTTTGTACCTATATTTTCAAAAATCAAACCAACTTTAGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGACTTTGTTTTTAGAATTTGGCTAAGAGTTCCAACACTTACTTAAACGAGATGATACCCTATTATAGAAGAATTAGGTGAAAATAAGCTTAACTTTCAAAAACTAAAAACAAAAAACAAAATGGTTATCAAACAAGACCTTAGTTTTTGGTTTTTGATTTTTGAAATTATGATTGTTTTTCCACAATTTTTTGATATCTTCTCCATAATGATTTTCATCTTTCTTGAGGTATCATTTAAGTGTAGTAAAATTTAAAAAACAAAAACAAGTTTTTGAAAACTATTTTTTTTAGTTTTCAAAACATAGCTTGATTTTTGAAAACATAGGTACAAATTACATAAAAAAACATAGATGTCCAGTAGTAGAAATAGTATTTTTAGACTTACTTTTCAAAAATAAAAAATAAAAAACCAAATGGTTATCAAGCGGGACCTTGATTATCAATCAGAACCTTAGTGTTGAAAAGTCTACATGGGGACCTATGTACTTGGATAGTGCAAAGAAAGTTGATGAGGTACAAAATGAGAGTTTTATGAGTTAAACTTGTATAACTTTTGAGATTAGTAAACTTCAAGAGCCTAATATAAACAAATCTAGTGGTACCAAGGTTCAACTTCTTAACTCTAGGCTACACAAATCTAATCAATGAGGCCAGCTAATGGTTTTGAATCCTGTCTAGGACAATTACTTGCATTTATACCACTTGGGTTGAATCTCAATCAAACTTCTTATAGTTTCTAAGCAGCAAAATGTCACCATTCTACTGAGATGAAACTATTTAGATCAAAAAGTACAGAGCTAAAGATCACCCTTTTCCCATCACCTGATGAGCTCTTGTACAATTTGGCTCAAATTCAACAGGGGTTTCTTTTCACTAAATTAAGTTCTGTTATGTTCTAACTGCATAAATGGTCAAGCCTTCACATGCCTGAGCTATATATGTAAATATGGTTTTTCTCTTTGGTTTACAGAATTCAAATGGATGGGAGTGCACAATGTGGTTGAGGTAACAAAGGAAGAGTATGAAAACTGTACCGGGGCAGGAACTCCTCATAGCACAAGCCCTGTGACAATCTCTCTTGATACCGCTGGCTCTAAATACTTTATCTGCTCCGTCGGTCCCCACTGTTCGTTGGGGCAGAAGTTGAGTGTGACCGTCGGATCTAGTAGCTCGAAAACTCCAGCAGCAACTTCACCAAGCTCTGTGCCAAGCTCGGCTCCCGCCTCTCTCCAGGCAAATGCTTTGTCTGCAGCTATGTTCGCCGTAGGCAGCATCTTCTTCACTTGTCTGTAA

mRNA sequence

ATGGCCAGCAAAATGAGTTTTGTTTGGTGTACGATCGCTTTCGCATGTGCAGCCATGGCTGCAACAGCAATGGCTGCAACCACCCATGTAGTTGGAGGTGACGAAGGCTGGAAAATTCCAACCGACGTGTCGTTTTACGCCAAGTGGACTGCCGATAAGACCTTCAATGTTGGTGACAAACTTGAATTCAAATGGATGGGAGTGCACAATGTGGTTGAGGTAACAAAGGAAGAGTATGAAAACTGTACCGGGGCAGGAACTCCTCATAGCACAAGCCCTGTGACAATCTCTCTTGATACCGCTGGCTCTAAATACTTTATCTGCTCCGTCGGTCCCCACTGTTCGTTGGGGCAGAAGTTGAGTGTGACCGTCGGATCTAGTAGCTCGAAAACTCCAGCAGCAACTTCACCAAGCTCTGTGCCAAGCTCGGCTCCCGCCTCTCTCCAGGCAAATGCTTTGTCTGCAGCTATGTTCGCCGTAGGCAGCATCTTCTTCACTTGTCTGTAA

Coding sequence (CDS)

ATGGCCAGCAAAATGAGTTTTGTTTGGTGTACGATCGCTTTCGCATGTGCAGCCATGGCTGCAACAGCAATGGCTGCAACCACCCATGTAGTTGGAGGTGACGAAGGCTGGAAAATTCCAACCGACGTGTCGTTTTACGCCAAGTGGACTGCCGATAAGACCTTCAATGTTGGTGACAAACTTGAATTCAAATGGATGGGAGTGCACAATGTGGTTGAGGTAACAAAGGAAGAGTATGAAAACTGTACCGGGGCAGGAACTCCTCATAGCACAAGCCCTGTGACAATCTCTCTTGATACCGCTGGCTCTAAATACTTTATCTGCTCCGTCGGTCCCCACTGTTCGTTGGGGCAGAAGTTGAGTGTGACCGTCGGATCTAGTAGCTCGAAAACTCCAGCAGCAACTTCACCAAGCTCTGTGCCAAGCTCGGCTCCCGCCTCTCTCCAGGCAAATGCTTTGTCTGCAGCTATGTTCGCCGTAGGCAGCATCTTCTTCACTTGTCTGTAA

Protein sequence

MASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL
Homology
BLAST of Tan0003435 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.5e-20
Identity = 52/111 (46.85%), Postives = 69/111 (62.16%), Query Frame = 0

Query: 31  VGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKW-MGVHNVVEVTKEEYENCTGAG--T 90
           VGGD  WK P+D  FY  W   KTF VGD+LEF +  G+H+V  VTK+ ++NC      +
Sbjct: 5   VGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPIS 64

Query: 91  PHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTV----GSSSSKTPAA 135
             +T PV I L+T G +Y+IC+VG HC +GQKLS+ V    G+    TP A
Sbjct: 65  HMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGGGATPGA 115

BLAST of Tan0003435 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 96.7 bits (239), Expect = 2.7e-19
Identity = 52/123 (42.28%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 26  ATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKW-MGVHNVVEV-TKEEYENCT 85
           +T H+VG + GW +P+  +FY++W A KTF VGD L+F +    HNV E+ TK+ ++ C 
Sbjct: 2   STVHIVGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACN 61

Query: 86  GAGTPHS---TSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTVGSSSSKTPAATSPSSV 144
              + +    TSPV   LD  G  YF+C+VG HCS GQKLS+ V ++++        SS 
Sbjct: 62  FVNSDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINVVAANATVSMPPPSSSP 121

BLAST of Tan0003435 vs. ExPASy Swiss-Prot
Match: Q07488 (Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-18
Identity = 63/148 (42.57%), Postives = 82/148 (55.41%), Query Frame = 0

Query: 5   MSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFK 64
           M+ V+ T+ F     AA  + A  + VG D  W  P D  FY  W   KTF VGD+LEF 
Sbjct: 1   MAGVFKTVTFLVLVFAAVVVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFD 60

Query: 65  W-MGVHNVVEVTKEEYENC-TGAGTPHST-SPVTISLDTAGSKYFICSVGPHCSLGQKLS 124
           +  G H+V  V++  +ENC       H T  PV I L+T G +YFIC+VG HC  GQKLS
Sbjct: 61  FAAGRHDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLS 120

Query: 125 VTV---GSSSSKTP---AATSPSSVPSS 144
           +TV   G++   TP   A  +P S PS+
Sbjct: 121 ITVVAAGATGGATPGAGATPAPGSTPST 148

BLAST of Tan0003435 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.3e-16
Identity = 45/104 (43.27%), Postives = 64/104 (61.54%), Query Frame = 0

Query: 27  TTHVVGGDEGWKIP--TDVSFYAKWTADKTFNVGDKLEFKW-MGVHNVVEVTKEEYENCT 86
           T + VG   GWK+P   DV +  KW ++KTF++GD L FK+    HNV +VT++ Y++C 
Sbjct: 1   TVYTVGDSAGWKVPFFGDVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCN 60

Query: 87  GAGTP---HSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTV 125
              TP   ++T    I+L T G KY+IC V  HC LGQK+ + V
Sbjct: 61  DT-TPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHINV 103

BLAST of Tan0003435 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 7.7e-14
Identity = 63/152 (41.45%), Postives = 81/152 (53.29%), Query Frame = 0

Query: 1   MASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDK 60
           MA   + V C +  A   MA  ++ AT + VG   GW I  D   Y+ W +DKTF VGD 
Sbjct: 1   MAFSNALVLCFL-LAIINMALPSL-ATVYTVGDTSGWVIGGD---YSTWASDKTFAVGDS 60

Query: 61  LEFKW-MGVHNVVEVTKEEYENCTGAG--TPHSTSPVTISLDTAGSKYFICSVGPHCSLG 120
           L F +  G H V EV + +Y++CT     +  ST   TI L  AG  YFIC V  H + G
Sbjct: 61  LVFNYGAGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGG 120

Query: 121 QKLSVTV--GSSSSKTPAATSPSSVPSSAPAS 148
            KLS+ V   S SS  P+AT PSS    +P+S
Sbjct: 121 MKLSIKVKASSGSSAAPSAT-PSSSGKGSPSS 146

BLAST of Tan0003435 vs. NCBI nr
Match: XP_022143139.1 (umecyanin-like [Momordica charantia])

HSP 1 Score: 219.9 bits (559), Expect = 1.6e-53
Identity = 115/173 (66.47%), Postives = 137/173 (79.19%), Query Frame = 0

Query: 1   MASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDK 60
           M   M  +W  +A A AAMAAT +AA TH VGGD+GW +P ++SFY  W +DKTFNVGDK
Sbjct: 30  MTIGMGSIWWVVALASAAMAATGIAAITHEVGGDKGWAVPQEMSFYTTWASDKTFNVGDK 89

Query: 61  LEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKL 120
           LEFKW G+H+V+EVTK+ +ENCT +GTP+ TSPV I L+T GSKYFIC+VGPHCSLGQKL
Sbjct: 90  LEFKWTGLHSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKL 149

Query: 121 SVTVGSSSSKTPAA-TSPSS----VPSSAPASLQANALSAAMFAVGSIFFTCL 169
           SVTVGS +S  PAA T+P++     PSSAPASL ANALSAA FA+GSI FTCL
Sbjct: 150 SVTVGSKNSAPPAAPTTPATPAAPAPSSAPASLLANALSAATFAIGSILFTCL 202

BLAST of Tan0003435 vs. NCBI nr
Match: XP_038890066.1 (umecyanin-like [Benincasa hispida])

HSP 1 Score: 196.8 bits (499), Expect = 1.5e-46
Identity = 111/170 (65.29%), Postives = 121/170 (71.18%), Query Frame = 0

Query: 1   MASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNVGD 60
           M S MSFVWC IA A A MA TA A+T H VG D+GW+ P  D SFY +W A+KTF VGD
Sbjct: 1   MGSTMSFVWCMIALALAVMATTATASTIHKVGDDQGWRFPKDDPSFYTRWAANKTFIVGD 60

Query: 61  KLEFKWMGVHNVVEVTKEEYENCTGAGTP-HSTSPVTISLDTAGSKYFICSVGPHCSLGQ 120
           KLEF W G HNVVEV K+EY NCT   TP H  SPVTISL TAGSKYFIC+V PHCS GQ
Sbjct: 61  KLEFIWTGTHNVVEVRKDEYGNCTEGETPLHELSPVTISLGTAGSKYFICAVVPHCSFGQ 120

Query: 121 KLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL 169
           KLSV V S  S  P    P+S PSSAP SL ANAL AAM  + S+FFTCL
Sbjct: 121 KLSVIVESDISTMP----PASAPSSAPTSLLANALYAAMLTITSMFFTCL 166

BLAST of Tan0003435 vs. NCBI nr
Match: XP_008456887.1 (PREDICTED: umecyanin-like [Cucumis melo])

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-40
Identity = 97/170 (57.06%), Postives = 118/170 (69.41%), Query Frame = 0

Query: 1   MASKMSFVWC-TIAFACAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNVG 60
           M S M FVWC  IA A AAMA T+ A+TT+ VG D+GWK+P  D + Y  W  +KTF VG
Sbjct: 1   MGSTMRFVWCLIIALALAAMATTSTASTTYKVGDDQGWKVPKDDPALYMAWPVNKTFTVG 60

Query: 61  DKLEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQ 120
           DKLEF W G HNV EVTKE+Y  C    T H  SPVTISLDT G KYFIC++ PHCS G+
Sbjct: 61  DKLEFTWTGTHNVAEVTKEDYTKCIEVKTVHEFSPVTISLDTPGPKYFICAIVPHCSFGE 120

Query: 121 KLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL 169
           +L++ V   +S TP A +P+  PSSAP+SL AN+L AAM  + S+FFT L
Sbjct: 121 RLTIVVEPDNSTTPPAPAPA--PSSAPSSLLANSLYAAMLTIASMFFTRL 168

BLAST of Tan0003435 vs. NCBI nr
Match: XP_004139411.1 (umecyanin [Cucumis sativus])

HSP 1 Score: 171.0 bits (432), Expect = 8.6e-39
Identity = 101/171 (59.06%), Postives = 117/171 (68.42%), Query Frame = 0

Query: 1   MASKMSFVWC-TIAFA-CAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNV 60
           M S MSFV C  IA A  AAMA T+ A+TT+ VG D+GWK+P  D + Y  W  +KTF V
Sbjct: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60

Query: 61  GDKLEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLG 120
           GDKLEF W G HNV EVTKEEY  C    T H  SPVTISLDT G KYFIC+V PHCS G
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120

Query: 121 QKLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL 169
           Q+L++ V   SS TP A  P+  PSSAP+SL AN+L AAM  + S+FFT L
Sbjct: 121 QRLTIVVEPDSSTTPPAPEPT--PSSAPSSLLANSLYAAMLTIVSMFFTRL 169

BLAST of Tan0003435 vs. NCBI nr
Match: KGN60711.2 (hypothetical protein Csa_019338 [Cucumis sativus])

HSP 1 Score: 154.5 bits (389), Expect = 8.4e-34
Identity = 94/185 (50.81%), Postives = 112/185 (60.54%), Query Frame = 0

Query: 2   ASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNVGDK 61
           A   +FV   +    AAMA T+ A+TT+ VG D+GWK+P  D + Y  W  +KTF VGDK
Sbjct: 203 AQGKTFVVGDVLALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTVGDK 262

Query: 62  L-----------------EFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGS 121
           L                 EF W G HNV EVTKEEY  C    T H  SPVTISLDT G 
Sbjct: 263 LGCISNLKLMKGTRILQKEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGP 322

Query: 122 KYFICSVGPHCSLGQKLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSI 169
           KYFIC+V PHCS GQ+L++ V   SS TP A  P+  PSSAP+SL AN+L AAM  + S+
Sbjct: 323 KYFICAVVPHCSFGQRLTIVVEPDSSTTPPAPEPT--PSSAPSSLLANSLYAAMLTIVSM 382

BLAST of Tan0003435 vs. ExPASy TrEMBL
Match: A0A6J1CNF9 (umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 7.9e-54
Identity = 115/173 (66.47%), Postives = 137/173 (79.19%), Query Frame = 0

Query: 1   MASKMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDK 60
           M   M  +W  +A A AAMAAT +AA TH VGGD+GW +P ++SFY  W +DKTFNVGDK
Sbjct: 30  MTIGMGSIWWVVALASAAMAATGIAAITHEVGGDKGWAVPQEMSFYTTWASDKTFNVGDK 89

Query: 61  LEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKL 120
           LEFKW G+H+V+EVTK+ +ENCT +GTP+ TSPV I L+T GSKYFIC+VGPHCSLGQKL
Sbjct: 90  LEFKWTGLHSVLEVTKDGFENCTSSGTPNGTSPVIIPLETVGSKYFICTVGPHCSLGQKL 149

Query: 121 SVTVGSSSSKTPAA-TSPSS----VPSSAPASLQANALSAAMFAVGSIFFTCL 169
           SVTVGS +S  PAA T+P++     PSSAPASL ANALSAA FA+GSI FTCL
Sbjct: 150 SVTVGSKNSAPPAAPTTPATPAAPAPSSAPASLLANALSAATFAIGSILFTCL 202

BLAST of Tan0003435 vs. ExPASy TrEMBL
Match: A0A1S3C4X2 (umecyanin-like OS=Cucumis melo OX=3656 GN=LOC103496698 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-40
Identity = 97/170 (57.06%), Postives = 118/170 (69.41%), Query Frame = 0

Query: 1   MASKMSFVWC-TIAFACAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNVG 60
           M S M FVWC  IA A AAMA T+ A+TT+ VG D+GWK+P  D + Y  W  +KTF VG
Sbjct: 1   MGSTMRFVWCLIIALALAAMATTSTASTTYKVGDDQGWKVPKDDPALYMAWPVNKTFTVG 60

Query: 61  DKLEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQ 120
           DKLEF W G HNV EVTKE+Y  C    T H  SPVTISLDT G KYFIC++ PHCS G+
Sbjct: 61  DKLEFTWTGTHNVAEVTKEDYTKCIEVKTVHEFSPVTISLDTPGPKYFICAIVPHCSFGE 120

Query: 121 KLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL 169
           +L++ V   +S TP A +P+  PSSAP+SL AN+L AAM  + S+FFT L
Sbjct: 121 RLTIVVEPDNSTTPPAPAPA--PSSAPSSLLANSLYAAMLTIASMFFTRL 168

BLAST of Tan0003435 vs. ExPASy TrEMBL
Match: A0A0A0LI34 (Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G006890 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.2e-39
Identity = 101/171 (59.06%), Postives = 117/171 (68.42%), Query Frame = 0

Query: 1   MASKMSFVWC-TIAFA-CAAMAATAMAATTHVVGGDEGWKIP-TDVSFYAKWTADKTFNV 60
           M S MSFV C  IA A  AAMA T+ A+TT+ VG D+GWK+P  D + Y  W  +KTF V
Sbjct: 1   MGSTMSFVSCLIIALALAAAMATTSTASTTYKVGDDQGWKVPKDDPAHYMAWPVNKTFTV 60

Query: 61  GDKLEFKWMGVHNVVEVTKEEYENCTGAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLG 120
           GDKLEF W G HNV EVTKEEY  C    T H  SPVTISLDT G KYFIC+V PHCS G
Sbjct: 61  GDKLEFTWTGTHNVAEVTKEEYTRCVEVKTVHEFSPVTISLDTPGPKYFICAVVPHCSFG 120

Query: 121 QKLSVTVGSSSSKTPAATSPSSVPSSAPASLQANALSAAMFAVGSIFFTCL 169
           Q+L++ V   SS TP A  P+  PSSAP+SL AN+L AAM  + S+FFT L
Sbjct: 121 QRLTIVVEPDSSTTPPAPEPT--PSSAPSSLLANSLYAAMLTIVSMFFTRL 169

BLAST of Tan0003435 vs. ExPASy TrEMBL
Match: A0A6P6FWF6 (uncharacterized protein LOC107428469 OS=Ziziphus jujuba OX=326968 GN=LOC107428469 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 4.6e-30
Identity = 74/140 (52.86%), Postives = 97/140 (69.29%), Query Frame = 0

Query: 25  AATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKWMGVHNVVEVTKEEYENCTG 84
           AA TH VG   GW IPT+++FY+ W   +TF+VGDKL+FKW G HNV +VTKEEYE+CT 
Sbjct: 293 AAVTHEVGDALGWTIPTEMNFYSSWADKQTFHVGDKLDFKWNGPHNVAKVTKEEYESCTA 352

Query: 85  AGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTVGSSSSKTPAATSPSSVPSSA 144
                S S V + L +AG+ YFIC+VG HC  GQKL+V + SS S +P+  SPSS  SS+
Sbjct: 353 TSADVSESSVVLPLTSAGTHYFICTVGKHCEHGQKLAVKISSSGSPSPSG-SPSSPTSSS 412

Query: 145 PASLQANALSAAMFAVGSIF 165
           PA+    A SA+  +VG++F
Sbjct: 413 PAAAAPQADSASSVSVGALF 431

BLAST of Tan0003435 vs. ExPASy TrEMBL
Match: A0A2C9VN81 (Phytocyanin domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G056400 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 4.1e-26
Identity = 68/140 (48.57%), Postives = 94/140 (67.14%), Query Frame = 0

Query: 9   WCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKWMGV 68
           W  IAF    +AA+A AATT+ VG   GW +PT++SFY  W ADK+F  GD L F W   
Sbjct: 7   WIIIAF----LAASADAATTYTVGDATGWAVPTNISFYDNWVADKSFEPGDSLVFNWTST 66

Query: 69  HNVVEVT-KEEYENCTGA-GTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTVGS 128
           HNV+EVT K EY++CT   G  + TSPVTI+L   G+ YFIC++GPHC+LGQK+++ VG+
Sbjct: 67  HNVLEVTSKAEYDSCTKTNGILNETSPVTINLTANGTLYFICTIGPHCTLGQKVTIKVGN 126

Query: 129 SSSKTPAATSPSSVPSSAPA 147
             S +P  ++ ++ P +  A
Sbjct: 127 GVSTSPPPSNSANPPFTISA 142

BLAST of Tan0003435 vs. TAIR 10
Match: AT5G20230.1 (blue-copper-binding protein )

HSP 1 Score: 94.7 bits (234), Expect = 7.3e-20
Identity = 63/148 (42.57%), Postives = 82/148 (55.41%), Query Frame = 0

Query: 5   MSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFK 64
           M+ V+ T+ F     AA  + A  + VG D  W  P D  FY  W   KTF VGD+LEF 
Sbjct: 1   MAGVFKTVTFLVLVFAAVVVFAEDYDVGDDTEWTRPMDPEFYTTWATGKTFRVGDELEFD 60

Query: 65  W-MGVHNVVEVTKEEYENC-TGAGTPHST-SPVTISLDTAGSKYFICSVGPHCSLGQKLS 124
           +  G H+V  V++  +ENC       H T  PV I L+T G +YFIC+VG HC  GQKLS
Sbjct: 61  FAAGRHDVAVVSEAAFENCEKEKPISHMTVPPVKIMLNTTGPQYFICTVGDHCRFGQKLS 120

Query: 125 VTV---GSSSSKTP---AATSPSSVPSS 144
           +TV   G++   TP   A  +P S PS+
Sbjct: 121 ITVVAAGATGGATPGAGATPAPGSTPST 148

BLAST of Tan0003435 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 78.6 bits (192), Expect = 5.4e-15
Identity = 61/166 (36.75%), Postives = 84/166 (50.60%), Query Frame = 0

Query: 19  MAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKWMGVHNVVEVTKEE 78
           +A  A+ A T  VG ++GW I  +   Y  W ++KTF VGD LEFK+   H+V  V K +
Sbjct: 16  VAVPAVFAVTFQVGDNDGWTIGVE---YTSWVSEKTFRVGDTLEFKYGPSHSVAVVNKAD 75

Query: 79  YENCTGAGTPHSTS--PVTISLDTAGSKYFICSVGPHCSLGQKLSVTV-----------G 138
           Y+ C  +    S S     I L   G+ +F+C    HCSLG KL+V V            
Sbjct: 76  YDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLGMKLAVQVLAAVSLEPPPSP 135

Query: 139 SSSSKTPAATSPS-SVPSSAPASLQA----NALSAAMFAVGSIFFT 167
           S+ S +P+A SPS S PS +P+   A    NA S  + + G I  T
Sbjct: 136 SAPSPSPSAPSPSPSAPSPSPSPGNAENLKNAASKGIMSYGKIVVT 178

BLAST of Tan0003435 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 78.2 bits (191), Expect = 7.1e-15
Identity = 48/125 (38.40%), Postives = 68/125 (54.40%), Query Frame = 0

Query: 26  ATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKW-MGVHNVVEVTKEEYENCTG 85
           AT H +GG  GW +   +     W A +TF VGD L F +    H+VVEVTK E+++C  
Sbjct: 23  ATDHTIGGPSGWTVGASL---RTWAAGQTFAVGDNLVFSYPAAFHDVVEVTKPEFDSCQA 82

Query: 86  AG--TPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTVGSSSSKTPAATSPSSVPS 145
                  +     + L T G +YFIC +  HCS G KL V V  +++  P A  P++VPS
Sbjct: 83  VKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVPTATVAPTAPLPNTVPS 142

Query: 146 -SAPA 147
            +AP+
Sbjct: 143 LNAPS 144

BLAST of Tan0003435 vs. TAIR 10
Match: AT3G27200.1 (Cupredoxin superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.6e-14
Identity = 58/169 (34.32%), Postives = 89/169 (52.66%), Query Frame = 0

Query: 4   KMSFVWCTIAFACAAMAATAMAATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEF 63
           KM  V   + F+      TA+AA  HV+GG +GW+   D   +  W++D++F VGD++ F
Sbjct: 2   KMQAVLVILVFSGLLSVKTALAA-RHVIGGSQGWEQSVD---FDSWSSDQSFKVGDQIVF 61

Query: 64  KWMGVHNVVEVTKE-EYENCTGAGTPHSTSP--VTISLDTAGSKYFICSVGPHCSLGQKL 123
           K+  +H+VVE+  E  Y++C    + +S S     + L   G++YF C    HC  G K+
Sbjct: 62  KYSELHSVVELGSETAYKSCDLGTSVNSLSSGNDVVKLSKTGTRYFACGTVGHCEQGMKI 121

Query: 124 SVTVGSSSSK---TPAATSPSSVPSSAPASLQANALSAA---MFAVGSI 164
            V V SS SK   +P+ +   S   S   S   + L A+   MF VGS+
Sbjct: 122 KVNVVSSDSKSASSPSGSGSGSDSGSGSGSSSGHGLRASTGYMFVVGSL 166

BLAST of Tan0003435 vs. TAIR 10
Match: AT5G07475.1 (Cupredoxin superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.3e-13
Identity = 52/144 (36.11%), Postives = 74/144 (51.39%), Query Frame = 0

Query: 26  ATTHVVGGDEGWKIPTDVSFYAKWTADKTFNVGDKLEFKWMGVHNVVEVTKEEYENC--T 85
           ATT+ VG   GW I +D+     WT+ K F+ GD L F++   H+V EV K+ Y+NC  T
Sbjct: 27  ATTYFVGDSSGWDISSDLE---SWTSGKRFSPGDVLMFQYSSTHSVYEVAKDNYQNCNTT 86

Query: 86  GAGTPHSTSPVTISLDTAGSKYFICSVGPHCSLGQKLSVTV-GSSSSKTP-----AATSP 145
            A    +    T++L   G+++F+C    HC  G +L V V G+  S+ P     AATS 
Sbjct: 87  DAIRTFTNGNTTVALSKPGNRFFVCGNRLHCFAGMRLLVNVEGNGPSQAPVGSPQAATSG 146

Query: 146 SSVPSSAPASLQANALSAAMFAVG 162
              PSS   +      S+A   VG
Sbjct: 147 ILQPSSKKNNPATGVASSAARFVG 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P428495.5e-2046.85Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1[more]
P296022.7e-1942.28Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3[more]
Q074881.0e-1842.57Blue copper protein OS=Arabidopsis thaliana OX=3702 GN=BCB PE=2 SV=2[more]
P003026.3e-1643.27Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1[more]
Q410017.7e-1441.45Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022143139.11.6e-5366.47umecyanin-like [Momordica charantia][more]
XP_038890066.11.5e-4665.29umecyanin-like [Benincasa hispida][more]
XP_008456887.12.1e-4057.06PREDICTED: umecyanin-like [Cucumis melo][more]
XP_004139411.18.6e-3959.06umecyanin [Cucumis sativus][more]
KGN60711.28.4e-3450.81hypothetical protein Csa_019338 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1CNF97.9e-5466.47umecyanin-like OS=Momordica charantia OX=3673 GN=LOC111013077 PE=4 SV=1[more]
A0A1S3C4X21.0e-4057.06umecyanin-like OS=Cucumis melo OX=3656 GN=LOC103496698 PE=4 SV=1[more]
A0A0A0LI344.2e-3959.06Phytocyanin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G006890... [more]
A0A6P6FWF64.6e-3052.86uncharacterized protein LOC107428469 OS=Ziziphus jujuba OX=326968 GN=LOC10742846... [more]
A0A2C9VN814.1e-2648.57Phytocyanin domain-containing protein OS=Manihot esculenta OX=3983 GN=MANES_06G0... [more]
Match NameE-valueIdentityDescription
AT5G20230.17.3e-2042.57blue-copper-binding protein [more]
AT3G60270.15.4e-1536.75Cupredoxin superfamily protein [more]
AT2G32300.17.1e-1538.40uclacyanin 1 [more]
AT3G27200.14.6e-1434.32Cupredoxin superfamily protein [more]
AT5G07475.12.3e-1336.11Cupredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 26..138
e-value: 3.9E-31
score: 109.4
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 25..126
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 37..118
e-value: 3.6E-20
score: 71.8
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 27..126
score: 36.993446
NoneNo IPR availablePANTHERPTHR33021:SF360OS08G0482600 PROTEINcoord: 5..137
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..16
score: 5.0
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 5..137
IPR028871Blue (type 1) copper protein, binding sitePROSITEPS00196COPPER_BLUEcoord: 102..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003435.1Tan0003435.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009055 electron transfer activity