Tan0003308 (gene) Snake gourd v1

Overview
NameTan0003308
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRING/U-box superfamily protein
LocationLG11: 3208480 .. 3210356 (-)
RNA-Seq ExpressionTan0003308
SyntenyTan0003308
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTTTCTCCATTCCTAAGCTTCATCTTCAACCTAACATGCCTCTTCCGGATCAAACCCACTCCGATTGTCCTTCTCCGGTACCGTCGATCCCATCCCTTCGCCGCGACGAGCTCCCCAACCCCATGGATTTCAACCCTTTTGCTACCGTCCCCTCCATCGTCACATCTGCTGACGATTCCCTCCCTTTTGAGACCGATTGGTTTTCCGACACCGATTCCGACTCCGAACGAGATGTCAGTTGCTTCGTGACTGATCTGTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTTGATTCTGTGCCAGACCCATTTACGCATTCTGCTAACGACTTGGGCATTTCGGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAGTCTGATACCCGGGTTTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAATAGTGGCCTTCGGGTTGTTGATGTGGATTCGGACTCGGATTCTGATAATGGAATTATTGGTGCTCTGCAACTCGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTCGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAGCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTCGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTAGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGGTTTTCCAATGACGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATACGAATTGGGAACGGGATAATGATGCTGAGGCGTACATAGCTGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGCTTTGAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCTCTTGTTGAGTTAAAAACAGAGAATATACTGACCGAGGAAGGGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGCTGTCACTATTACCATGATGATTGCATTCTTCCATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCAACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGGTACAATTTTGAACTAATAGCTTAAGATTAGTGGAATCTGTGAAATGCAACCTGTGAATAGCGTCTCTTTTAGATGAAGAAAGTTGCTTACGATTTTCCATTTGTAAAGGTTTTGTGGTAAGTATTTCTTTTAAAAATTCTCCGCGTGGAACTACTAGCACTGCTGTTGGTACACTCCAATTATCTCCACAGGTATCAATTGTGATTCAATGCTTGGTAAGTTTTAGTCATTAATAGTTGTAGGAAATTACATTGCCTCTTACCTTGTTGAATTCAATTTAGGAATTATAATGTTTGAGACATGACATACTAATGATCATTTTACTACTACAAGTGACGGGCTTTGAAATTGTATAGTTGTGAAGTATATTACATTAGTATGTTTGTATGTATGCTTGAGTATCTGGTGGTAAACTTGTAAAAATAGGAACCTCCTGGTATGAGATTTCGTGTGGCGCAATTGCTTGATTTTGGATTAATTCTGATAACTTAGCTGATTAAGCATGGGAGGACGATACTTCTTGTCATCGGGTTTTGGTTCTTAATACTTACTTACTCATCCACATTGGACATAGAAATCTTAGTGGATATGCTTTGGCCTTTTGA

mRNA sequence

ATGGCTTTTTTCTCCATTCCTAAGCTTCATCTTCAACCTAACATGCCTCTTCCGGATCAAACCCACTCCGATTGTCCTTCTCCGGTACCGTCGATCCCATCCCTTCGCCGCGACGAGCTCCCCAACCCCATGGATTTCAACCCTTTTGCTACCGTCCCCTCCATCGTCACATCTGCTGACGATTCCCTCCCTTTTGAGACCGATTGGTTTTCCGACACCGATTCCGACTCCGAACGAGATGTCAGTTGCTTCGTGACTGATCTGTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTTGATTCTGTGCCAGACCCATTTACGCATTCTGCTAACGACTTGGGCATTTCGGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAGTCTGATACCCGGGTTTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAATAGTGGCCTTCGGGTTGTTGATGTGGATTCGGACTCGGATTCTGATAATGGAATTATTGGTGCTCTGCAACTCGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTCGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAGCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTCGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTAGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGGTTTTCCAATGACGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATACGAATTGGGAACGGGATAATGATGCTGAGGCGTACATAGCTGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGCTTTGAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCTCTTGTTGAGTTAAAAACAGAGAATATACTGACCGAGGAAGGGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGCTGTCACTATTACCATGATGATTGCATTCTTCCATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCAACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGTATTTCTTTTAAAAATTCTCCGCGTGGAACTACTAGCACTGCTGTTGGTACACTCCAATTATCTCCACAGGTATCAATTGTGATTCAATGCTTGGACGATACTTCTTGTCATCGGGTTTTGGTTCTTAATACTTACTTACTCATCCACATTGGACATAGAAATCTTAGTGGATATGCTTTGGCCTTTTGA

Coding sequence (CDS)

ATGGCTTTTTTCTCCATTCCTAAGCTTCATCTTCAACCTAACATGCCTCTTCCGGATCAAACCCACTCCGATTGTCCTTCTCCGGTACCGTCGATCCCATCCCTTCGCCGCGACGAGCTCCCCAACCCCATGGATTTCAACCCTTTTGCTACCGTCCCCTCCATCGTCACATCTGCTGACGATTCCCTCCCTTTTGAGACCGATTGGTTTTCCGACACCGATTCCGACTCCGAACGAGATGTCAGTTGCTTCGTGACTGATCTGTTCGAGAATCGTCGTTTCACCGATCATTTGCACGCTCTCAGCGATCACGATCGAGGTCTTGATTCTGTGCCAGACCCATTTACGCATTCTGCTAACGACTTGGGCATTTCGGGATTTGAAGATAGCGGTGAGATTGAATCTAATTATATTGAGGAGCTGTGGTCGACGTTTGCTGTGGAGTCTGATACCCGGGTTTCGGAGGTGGATGTGAGCATGCAAGTTGCGGATTCGATTAATAGTGGCCTTCGGGTTGTTGATGTGGATTCGGACTCGGATTCTGATAATGGAATTATTGGTGCTCTGCAACTCGTGACAGGGGATGATGGCGAAAATGATGTTAATTGGGTCGAAAGTCCGAATCCTCTCTCGGATAACTTCAGCTTTTGTTTTCAGCAGAGGATTTCGTACGAGGATTTTGAATGGGAGGAGGTCGAGGAACGGGTGGACGAAAGGGAAATTTCGAGCGTAGTAGTGGATGGAGCTGAGGAATTGTCTCTTGCATCTGGGTTTTCCAATGACGAAGAATCAGGAGAAGAGGCGGGTCGATGGGAAATTCTATTGGTAATGAACGATATTGGGAGGAATACGAATTGGGAACGGGATAATGATGCTGAGGCGTACATAGCTGACCAGGATGATTACATGTATGCAGCAGAATATGATACCCTTTTTGGACAATTCGTTGAAAATGAGAATGCTTTGAAGGGTAGTCCTCCAGCAGCTAAGAGTGCTGTGGAGAATCTTCCTCTTGTTGAGTTAAAAACAGAGAATATACTGACCGAGGAAGGGGTTATTTGTGCTGTTTGTAAGGATAAATTTTCAATGGAGGAGAAGGTGAGGAAGCTTCCCTGCTGTCACTATTACCATGATGATTGCATTCTTCCATGGCTGAATATCCGAAACACATGTCCTGTTTGTCGGTATGAGTTGCCAACCGACGATCCAGATTATGAGCGTAGGAAGAGTCAAAGGGCGAGTGGTGGCCTGCAAACGGATTTGCAGGTCAGTATTTCTTTTAAAAATTCTCCGCGTGGAACTACTAGCACTGCTGTTGGTACACTCCAATTATCTCCACAGGTATCAATTGTGATTCAATGCTTGGACGATACTTCTTGTCATCGGGTTTTGGTTCTTAATACTTACTTACTCATCCACATTGGACATAGAAATCTTAGTGGATATGCTTTGGCCTTTTGA

Protein sequence

MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSADDSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSANDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSDSDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDEREISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQDDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTDLQVSISFKNSPRGTTSTAVGTLQLSPQVSIVIQCLDDTSCHRVLVLNTYLLIHIGHRNLSGYALAF
Homology
BLAST of Tan0003308 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 2.7e-27
Identity = 59/132 (44.70%), Postives = 83/132 (62.88%), Query Frame = 0

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DY +    + L  Q  EN+    G+PPA+KSA++ LP V++ T+++L  E   CAVC D+
Sbjct: 164 DYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV-TKDMLKSEMNQCAVCMDE 223

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           F     V+++PC H +H DC+LPWL + N+CPVCR+ELPTDDPDYE R       G   D
Sbjct: 224 FEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENRSQGSQGSG---D 283

Query: 421 LQVSISFKNSPR 433
            Q S+  + +PR
Sbjct: 284 GQGSVEGQQTPR 291

BLAST of Tan0003308 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.6e-27
Identity = 62/126 (49.21%), Postives = 80/126 (63.49%), Query Frame = 0

Query: 297 ADQDDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAV 356
           A+  DY      + L  Q  EN+    G+PPA+KSA+E LPLV + T++ L  E   CAV
Sbjct: 168 ANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNI-TKSNLNSEFNQCAV 227

Query: 357 CKDKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYER--RKSQRAS 416
           C D F    + +++PC H YH DC+LPWL + N+CPVCR+ELPTDDPDYER  R +Q  S
Sbjct: 228 CMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTS 287

Query: 417 GGLQTD 421
           GG   D
Sbjct: 288 GGNDGD 292

BLAST of Tan0003308 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 4.8e-24
Identity = 79/220 (35.91%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 222 ISYED--FEWE-----EVEERVDEREISSVVVDGAEELSLASGFSNDEESG-EEAGRWEI 281
           +SYED  F  E     E+++  DE E      +  EE +L      DEE       R+ +
Sbjct: 100 LSYEDDFFRLELNSRNEIDDDEDEDEDDGDEEEEDEEENLTVNDEEDEEDDLRRRNRFPL 159

Query: 282 LLVMNDIGRN--TNWE------RDN------DAEAYIADQDDYM-YAAEYDTLFGQFVEN 341
               +  GRN   +W        DN      +++ Y  +  DY+  AA Y+ L     E 
Sbjct: 160 TTTQSRTGRNRILDWAEILMGIEDNSIEFRMESDRYAGNPADYIDDAAGYEALLQNLAEG 219

Query: 342 E----NALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDKFSMEEKVRKLPCCH 401
           +       +G+PPAAKSA+E L   E+ +     E  ++CAVCKD   M E  +KLPC H
Sbjct: 220 DGGGGGGRRGAPPAAKSAIEALETFEVSSSE--GEMVMVCAVCKDGMVMGETGKKLPCGH 279

Query: 402 YYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAS 415
            YH DCI+PWL  RN+CPVCR++L TDD +YE  + +R S
Sbjct: 280 CYHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEERKKRTS 317

BLAST of Tan0003308 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.6e-22
Identity = 43/77 (55.84%), Postives = 59/77 (76.62%), Query Frame = 0

Query: 326 PPAAKSAVENLPLVELKTENILTEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCILPWL 385
           PPA+KSA+E LPL+E+   ++L++    CAVCK+ F ++   R++PC H YH DCILPWL
Sbjct: 173 PPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWL 232

Query: 386 NIRNTCPVCRYELPTDD 403
            IRN+CPVCR+ELP +D
Sbjct: 233 AIRNSCPVCRHELPAED 249

BLAST of Tan0003308 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 2.4e-20
Identity = 43/78 (55.13%), Postives = 61/78 (78.21%), Query Frame = 0

Query: 324 GSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCILP 383
           G+PPA+KSA+E+LP VE+   +I +E    CAVC + F  E + R++PC H +HDDCI+P
Sbjct: 171 GNPPASKSAIESLPRVEISDCHIGSEAN--CAVCTEIFETETEAREMPCKHLFHDDCIVP 230

Query: 384 WLNIRNTCPVCRYELPTD 402
           WL+IRN+CPVCR+ELP++
Sbjct: 231 WLSIRNSCPVCRFELPSE 246

BLAST of Tan0003308 vs. NCBI nr
Match: XP_022992752.1 (uncharacterized protein LOC111488995 isoform X1 [Cucurbita maxima])

HSP 1 Score: 791.2 bits (2042), Expect = 5.1e-225
Identity = 398/460 (86.52%), Postives = 423/460 (91.96%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           M F SIPKL+LQPN+ LPDQTHSD PSPVPS+PSLRRD+LPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDS PDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESD+RVSEVDVSM VADS  SGLR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRISYEDFEWEEVEERVDER 240
           SDNG+IGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQ QRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSN+EESGEEAGRWEILLVMN+I RNTNWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKGSPPAAKS+VENLPLVELKTEN+LTEE V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQV----SISFKNSPRGTTSTAVGTLQLSPQVSIVIQCL 456
           DL+V     ISFK+ P GTTS AV TL+LS QVSIVIQ L
Sbjct: 421 DLRVRFWGKISFKSFPPGTTSIAVDTLKLSSQVSIVIQML 460

BLAST of Tan0003308 vs. NCBI nr
Match: XP_022921923.1 (uncharacterized protein LOC111430034 [Cucurbita moschata])

HSP 1 Score: 783.9 bits (2023), Expect = 8.1e-223
Identity = 389/428 (90.89%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL ALS+HD  LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESD+RVSEVDVSMQVADSINS LRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            +NGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL++ASGFSNDEESGEEAGRWEILLVM+DIG NTNWERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. NCBI nr
Match: KAG7033230.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-222
Identity = 389/428 (90.89%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE+PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEVPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSER VSC VTDLFEN RFTDHL ALS+HD  LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERHVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESD+RVSEVDVSMQVADSINS LRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            DNGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL++ASGFSNDEESGEEAGRWEILLVM+DIG NTNWERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. NCBI nr
Match: XP_022989863.1 (uncharacterized protein LOC111486927 [Cucurbita maxima])

HSP 1 Score: 776.5 bits (2004), Expect = 1.3e-220
Identity = 386/428 (90.19%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE PNPMDFNPFATVPSIVT  D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVTPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL  LS+HD  LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLTLSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVES +RVSEVDVSMQVADSINS LRVVDVDSD+D
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESGSRVSEVDVSMQVADSINSDLRVVDVDSDTD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            DNGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL+LAS FSNDEESGEEAGRWEILLVM+DIG NTNWERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTLASDFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYD LFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDALFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQR SGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRTSGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. NCBI nr
Match: XP_023526783.1 (uncharacterized protein LOC111790174 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 775.8 bits (2002), Expect = 2.2e-220
Identity = 388/428 (90.65%), Postives = 399/428 (93.22%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL ALS+HDR LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDRDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESD+R SEVDVSMQVADSINS LRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRDSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            DNGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL+LASGFSNDEESGEEAGRWEILLVM+DIG NTN ERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTLASGFSNDEESGEEAGRWEILLVMDDIGSNTNRERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCI+ WLNIRNTCPVCRYELPTDDPDYERRKSQRASGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCIVQWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. ExPASy TrEMBL
Match: A0A6J1JQS9 (uncharacterized protein LOC111488995 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.5e-225
Identity = 398/460 (86.52%), Postives = 423/460 (91.96%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           M F SIPKL+LQPN+ LPDQTHSD PSPVPS+PSLRRD+LPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDS PDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESD+RVSEVDVSM VADS  SGLR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRISYEDFEWEEVEERVDER 240
           SDNG+IGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQ QRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSN+EESGEEAGRWEILLVMN+I RNTNWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKGSPPAAKS+VENLPLVELKTEN+LTEE V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQV----SISFKNSPRGTTSTAVGTLQLSPQVSIVIQCL 456
           DL+V     ISFK+ P GTTS AV TL+LS QVSIVIQ L
Sbjct: 421 DLRVRFWGKISFKSFPPGTTSIAVDTLKLSSQVSIVIQML 460

BLAST of Tan0003308 vs. ExPASy TrEMBL
Match: A0A6J1E557 (uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC111430034 PE=4 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.9e-223
Identity = 389/428 (90.89%), Postives = 401/428 (93.69%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE PNPMDFNPFATVPSIV   D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVAPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL ALS+HD  LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLALSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVESD+RVSEVDVSMQVADSINS LRVVDVDSDSD
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESDSRVSEVDVSMQVADSINSDLRVVDVDSDSD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            +NGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YENGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL++ASGFSNDEESGEEAGRWEILLVM+DIG NTNWERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTIASGFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYDTLFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDTLFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. ExPASy TrEMBL
Match: A0A6J1JRJ8 (uncharacterized protein LOC111486927 OS=Cucurbita maxima OX=3661 GN=LOC111486927 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.3e-221
Identity = 386/428 (90.19%), Postives = 397/428 (92.76%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           MAFFSIPKLHLQPNMPLPDQ  SD PSPVPSI S RRDE PNPMDFNPFATVPSIVT  D
Sbjct: 1   MAFFSIPKLHLQPNMPLPDQPRSDSPSPVPSITSFRRDEFPNPMDFNPFATVPSIVTPPD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DSL FETDWFSDTDSDSERDVSC VTDLFEN RFTDHL  LS+HD  LDS PDPFTHSAN
Sbjct: 61  DSLRFETDWFSDTDSDSERDVSCLVTDLFENHRFTDHLLTLSNHDHDLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFEDSG+IESNY EELWSTFAVES +RVSEVDVSMQVADSINS LRVVDVDSD+D
Sbjct: 121 DLGISGFEDSGDIESNYTEELWSTFAVESGSRVSEVDVSMQVADSINSDLRVVDVDSDTD 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEEVEERVDERE 240
            DNGIIGALQ VTGDDGENDVNWVESPNPLSDNFSFCFQQR SYEDFEWEEVEERVDERE
Sbjct: 181 YDNGIIGALQFVTGDDGENDVNWVESPNPLSDNFSFCFQQRNSYEDFEWEEVEERVDERE 240

Query: 241 ISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQD 300
           ISSVVV+GAEEL+LAS FSNDEESGEEAGRWEILLVM+DIG NTNWERDNDAEAY+ADQD
Sbjct: 241 ISSVVVEGAEELTLASDFSNDEESGEEAGRWEILLVMDDIGSNTNWERDNDAEAYVADQD 300

Query: 301 DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDK 360
           DYMYAAEYD LFGQFVENENALKG PPAAKSA+ENLPLVELKTENILTEE VICAVCKDK
Sbjct: 301 DYMYAAEYDALFGQFVENENALKGCPPAAKSAMENLPLVELKTENILTEEVVICAVCKDK 360

Query: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQTD 420
           FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQR SGG QTD
Sbjct: 361 FSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRTSGGQQTD 420

Query: 421 LQVSISFK 429
           LQV  +F+
Sbjct: 421 LQVRYNFE 428

BLAST of Tan0003308 vs. ExPASy TrEMBL
Match: A0A6J1JWL4 (uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 7.2e-217
Identity = 376/429 (87.65%), Postives = 401/429 (93.47%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           M F SIPKL+LQPN+ LPDQTHSD PSPVPS+PSLRRD+LPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDS PDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESD+RVSEVDVSM VADS  SGLR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRISYEDFEWEEVEERVDER 240
           SDNG+IGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQ QRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSN+EESGEEAGRWEILLVMN+I RNTNWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKGSPPAAKS+VENLPLVELKTEN+LTEE V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVSISFK 429
           DL+V  +F+
Sbjct: 421 DLRVRYNFE 429

BLAST of Tan0003308 vs. ExPASy TrEMBL
Match: A0A6J1JYD4 (uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111488995 PE=4 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.2e-216
Identity = 377/431 (87.47%), Postives = 400/431 (92.81%), Query Frame = 0

Query: 1   MAFFSIPKLHLQPNMPLPDQTHSDCPSPVPSIPSLRRDELPNPMDFNPFATVPSIVTSAD 60
           M F SIPKL+LQPN+ LPDQTHSD PSPVPS+PSLRRD+LPN MDFNPFAT+ SIVTSAD
Sbjct: 1   MDFLSIPKLNLQPNVALPDQTHSDSPSPVPSVPSLRRDDLPNTMDFNPFATISSIVTSAD 60

Query: 61  DSLPFETDWFSDTDSDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN 120
           DS PF+TDWFSD DS+SERDVSCFVTDLFENRRFTDHLHA SD+DRGLDS PDPFTHSAN
Sbjct: 61  DSFPFDTDWFSDADSESERDVSCFVTDLFENRRFTDHLHAFSDYDRGLDSGPDPFTHSAN 120

Query: 121 DLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSD 180
           DLGISGFED+GEIES+YIEELWS FA+ESD+RVSEVDVSM VADS  SGLR+VDVDSDS+
Sbjct: 121 DLGISGFEDTGEIESHYIEELWSAFAMESDSRVSEVDVSMPVADSTTSGLRIVDVDSDSE 180

Query: 181 SDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQ-QRISYEDFEWEEVEERVDER 240
           SDNG+IGALQLVTGDDGEND NWVE+PNPLSD+FSFCFQ QRI YEDFEWEEVEERVDER
Sbjct: 181 SDNGMIGALQLVTGDDGENDANWVENPNPLSDSFSFCFQGQRIPYEDFEWEEVEERVDER 240

Query: 241 EISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYIADQ 300
           +  S VVDGAEELSLASGFSN+EESGEEAGRWEILLVMN+I RNTNWERDNDAEAYIADQ
Sbjct: 241 DNLSAVVDGAEELSLASGFSNEEESGEEAGRWEILLVMNNIVRNTNWERDNDAEAYIADQ 300

Query: 301 DDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKD 360
           DDYMYA EYDTLFGQFVENENALKGSPPAAKS+VENLPLVELKTEN+LTEE V CAVCKD
Sbjct: 301 DDYMYAGEYDTLFGQFVENENALKGSPPAAKSSVENLPLVELKTENLLTEELVACAVCKD 360

Query: 361 KFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRASGGLQT 420
           KFSMEEKVRKLPC HYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRA GGLQT
Sbjct: 361 KFSMEEKVRKLPCAHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAGGGLQT 420

Query: 421 DLQVSISFKNS 431
           DL+V   F  S
Sbjct: 421 DLRVRFPFHPS 431

BLAST of Tan0003308 vs. TAIR 10
Match: AT3G02340.1 (RING/U-box superfamily protein )

HSP 1 Score: 186.8 bits (473), Expect = 4.1e-47
Identity = 123/336 (36.61%), Postives = 183/336 (54.46%), Query Frame = 0

Query: 109 DSVPDP-------FTHSANDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDV--- 168
           DS PDP       F   ++D+ ++ + +SG + +     +W  +  + D    E+ +   
Sbjct: 69  DSEPDPNSCPIDFFDRDSSDVDVAEYLESGGLTAGDY-NIWGFYDPKEDEEEEEIVLGTS 128

Query: 169 --SMQVADSINSGLRVVDVDSDSDSDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSF 228
              +Q  DS   GLRV  +DSDSD ++G+   +   +  +  ND   VE    L   +  
Sbjct: 129 GSDLQPGDSGEQGLRVTGIDSDSDCEDGVFDFISEDSSGNRGNDSGRVEVGTGLPPVWDH 188

Query: 229 CFQQRISYEDFEWEEVEERVDEREISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLV 288
            F +     D EWEEV+  ++    S    D  EE  L+S   +DEE   E   W++LL 
Sbjct: 189 LFGEGTVLADEEWEEVQNAINWTAFSG-PEDEDEEDELSSLSRDDEEEDHELD-WQVLLT 248

Query: 289 MNDI--------GRNTNWERDNDAEAYI----ADQDDYMYAAEY--DTLFGQFVENENAL 348
           +N++        G   N + D D + Y+     D+ D  ++  Y  D + GQ  ++E  +
Sbjct: 249 VNNVVNYIEQAEGIMLNPD-DIDPDYYLYLSGLDEFDENHSGHYDADAILGQMFDDETGI 308

Query: 349 KGSPPAAKSAVENLPLVELKTENILTEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCIL 408
           +G+PPAAKS +++LP+VEL  E  L +   +CAVCKD+  +EEKVR+LPC H+YH +CI+
Sbjct: 309 RGNPPAAKSVIQDLPVVELAVEE-LDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECII 368

Query: 409 PWLNIRNTCPVCRYELPTDDPDYERRK-SQRASGGL 418
           PWL IRNTCPVCRYELPTDD +YER K S+R   GL
Sbjct: 369 PWLGIRNTCPVCRYELPTDDLEYERHKSSERGDTGL 399

BLAST of Tan0003308 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.3 bits (399), Expect = 1.6e-38
Identity = 108/296 (36.49%), Postives = 162/296 (54.73%), Query Frame = 0

Query: 148 ESDTRVSEVDVSMQVADSINSG--LRVVDVDSDSDSDNGIIGALQLVTGDDGENDVNWVE 207
           + D  V   D  ++  D  +SG  +R++ V+S SD D+G+    +L   D  E DV +V 
Sbjct: 82  DDDFFVGRTDFGLEFRDIASSGNDIRLITVESGSDDDDGVENERELWGIDLNEEDV-YVN 141

Query: 208 SPNPLSDN-----FSFCF------QQRISYEDFEWEEVEER-----VDEREISS------ 267
             +   D+        C+       + ++ ++F+WEEV         DEREI S      
Sbjct: 142 DDDEYEDDDVSVTIPLCWDSLQLEDREVTADEFDWEEVGGGGGGGVDDEREIRSGFAQID 201

Query: 268 ----VVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRNTNWERDNDAEAYI-AD 327
                ++  +  +SL    + +   G     WE+LL   +     N++ DN  E YI  D
Sbjct: 202 MNDESLISASPIISLEGLVTRERAEGSGNLGWEVLL---NHTLEINFDVDN-RELYIGGD 261

Query: 328 QDDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEEGVICAVCK 387
            DDY+   +YD LF QF + E ++ G PP +KS + NLP+V L+ EN   + G++CAVCK
Sbjct: 262 HDDYV--QDYDMLFEQFADAEVSVIGLPPTSKSFLNNLPVVLLEGEND-DDGGLVCAVCK 321

Query: 388 DKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKSQRAS 415
           D+ ++  K  +LPC H YH +CI+PWL +RNTCPVCRYELPTDD +YE+RK+QR +
Sbjct: 322 DEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVCRYELPTDDAEYEQRKTQRTT 369

BLAST of Tan0003308 vs. TAIR 10
Match: AT5G15820.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 3.0e-34
Identity = 110/306 (35.95%), Postives = 154/306 (50.33%), Query Frame = 0

Query: 115 FTHSANDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVD 174
           F  S +D G     DS   E  +I + +   + E DT V EV VS     S      + D
Sbjct: 62  FLESNSDSG----SDSMYAEPEFI-DFFDRESYEVDT-VREVCVSSNQRVSTPGYFNIWD 121

Query: 175 VDSDSDSDNGI---IGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEE 234
            D D     G+    G+ QL  GD G   V       P+  N  F  +  +  E  EWE 
Sbjct: 122 QDVDLGLGIGLGSRSGSGQL-PGDSGGVGVEVGRGVTPVEYNL-FGEEAMVVDEVLEWEN 181

Query: 235 VEERVD-EREISSVVVDGAEE-------LSLASGFSNDEESGEEAGRWEILLVMNDIGRN 294
               +   +E +   ++G EE       +  A+   +D         WEILL  N     
Sbjct: 182 FNNAIHLVQEPAYASMEGEEEEEEDEVVMEFAASIYSD--------AWEILLYDN---MT 241

Query: 295 TNWERDNDAEAYIADQDDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKT 354
            +   D D E ++   D Y    +Y+ + GQ  +NE  +KG+PPA+KS V+ LP VEL  
Sbjct: 242 NSAPMDLDVEVWLDSVDGYA-PMDYNAIIGQMFDNETGIKGTPPASKSVVDGLPDVELTI 301

Query: 355 ENILTEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDP 410
           E  L+   ++CA+CKD+   +EKV++LPC HYYH +CI+PWL IRNTCPVCR+ELPTDD 
Sbjct: 302 EE-LSSVSIVCAICKDEVVFKEKVKRLPCKHYYHGECIIPWLGIRNTCPVCRHELPTDDL 346

BLAST of Tan0003308 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 3.5e-30
Identity = 103/326 (31.60%), Postives = 159/326 (48.77%), Query Frame = 0

Query: 115 FTHSANDLGISGFEDSGEIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVD 174
           F+ S +D     F++ GE ESN    L+     ++DT          ++ S   G     
Sbjct: 139 FSESESD----DFDNFGETESNASFSLYRFPHADNDT----------ISFSAYGG----- 198

Query: 175 VDSDSDSDNGIIGALQLVTGDDGENDVNWVESPNPLSDNFSFCFQQRISYEDFEWEE--V 234
            +SD+ +D        +    D  +D+++    +P+    +   Q     ED EWEE   
Sbjct: 199 -ESDASTDR----HADIFVQPDDRSDIDFDTDIDPMRAGLN---QWNSDEEDREWEEGAG 258

Query: 235 EERVDEREISSVVVDGAEELSLASGFSNDEESGEEAGRWEILLVMNDIGRN--TNWERDN 294
              V      + +   +E  S  + F + E   E + R  I+   + + RN  T  E D 
Sbjct: 259 PSGVAGTRYRNYLASPSESYSSMTRFDSPEL--ERSFRQRIIERRHSLSRNIFTGLE-DL 318

Query: 295 DAEAYIADQDDYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTENILTEE 354
           D   Y A+  DY+    +D L  Q  E++N+ +G+PPA+ S V NLP V +  E+++  +
Sbjct: 319 DFSPYAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVIIAEEHVM--K 378

Query: 355 GVICAVCKDKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTDDPDYERRKS 414
           G++CA+CK+ FS+  +  +LPC H YH  CI+PWL+ RN+CP+CRYELPTDD DYE    
Sbjct: 379 GLVCAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTDDKDYEE--- 424

Query: 415 QRASGGLQTDLQVSISFKNSPRGTTS 437
                G +  L VS    +S  GT S
Sbjct: 439 -----GKRNVLDVSEDSSSSDDGTES 424

BLAST of Tan0003308 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 1.1e-28
Identity = 124/419 (29.59%), Postives = 192/419 (45.82%), Query Frame = 0

Query: 17  LPDQTHSDCPSPVPS-IPSLRRDELPNPMDFNP-FATVPSIVTSADDSLPFETDWFSDTD 76
           L DQ+ S    P+P  I S   D   +  DF+    +V  +V + DD L           
Sbjct: 30  LRDQSFSQILYPLPHWIQSDDGDLYISETDFSSGDVSVSDLVFTTDDGLDL--------- 89

Query: 77  SDSERDVSCFVTDLFENRRFTDHLHALSDHDRGLDSVPDPFTHSAN---DLGISGFEDSG 136
                D   FV DLF  R     +  L D+D G+D   +      N   D G+     +G
Sbjct: 90  ----LDRRSFVMDLFHQRVEQSQVTPL-DND-GIDDFENYEMREDNVELDFGLEMESGNG 149

Query: 137 EIESNYIEELWSTFAVESDTRVSEVDVSMQVADSINSGLRVVDVDSDSDSDNGIIGALQL 196
            ++S+ +  L           +   DV + V    +S     D + D + +   I  + L
Sbjct: 150 FVDSDLVVNLNEHVDGMRLLGIESGDVIVTVESCFDS-----DDEDDQEKEESEIWGIDL 209

Query: 197 -----VTGDDGENDVNWVESPNPLSDNFSFCFQQ------RISYEDFEWEEVEERVDERE 256
                   DD +ND    +     S     C+         I+ ED EWEEV+   DERE
Sbjct: 210 NEEDEYVNDDDDND----DEEEDASVTIPLCWDSLQLEDLGINNEDCEWEEVDSD-DERE 269

Query: 257 ISSVVVDGAE----ELSLASGFSND-----EESGEEAGRWEILLVMNDIGRNTNWERDND 316
           + SV+ +  +     +S+A+  S +     E  G     WE+LL    +  N + + +++
Sbjct: 270 VLSVLAEADDNNSVSVSVAATISLEDLAISERRGSSNLGWEVLLNSRSLEFNLD-DAESN 329

Query: 317 AEAYIADQD-------DYMYAAEYDTLFGQFVENENALKGSPPAAKSAVENLPLVELKTE 376
            E YI D D       DY++  EY+ LF   + +     G PPA+KS ++NL +  L  E
Sbjct: 330 LELYIGDIDHEEEDYEDYLHTTEYEMLFEAEISSG---IGKPPASKSFIKNLKVSPLSNE 389

Query: 377 NIL--TEEGVICAVCKDKFSMEEKVRKLPCCHYYHDDCILPWLNIRNTCPVCRYELPTD 402
           +++   ++ V CAVCK++  + ++V +LPC H YH +CI+PWL IRNTCPVCR+ELP+D
Sbjct: 390 DVMENDDDAVCCAVCKEEMIVGKEVAELPCRHKYHSECIVPWLGIRNTCPVCRFELPSD 419

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN72.7e-2744.70E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH304.6e-2749.21E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q9SPL24.8e-2435.91E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
O222837.6e-2255.84Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T52.4e-2055.13E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_022992752.15.1e-22586.52uncharacterized protein LOC111488995 isoform X1 [Cucurbita maxima][more]
XP_022921923.18.1e-22390.89uncharacterized protein LOC111430034 [Cucurbita moschata][more]
KAG7033230.11.4e-22290.89E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022989863.11.3e-22090.19uncharacterized protein LOC111486927 [Cucurbita maxima][more]
XP_023526783.12.2e-22090.65uncharacterized protein LOC111790174 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1JQS92.5e-22586.52uncharacterized protein LOC111488995 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E5573.9e-22390.89uncharacterized protein LOC111430034 OS=Cucurbita moschata OX=3662 GN=LOC1114300... [more]
A0A6J1JRJ86.3e-22190.19uncharacterized protein LOC111486927 OS=Cucurbita maxima OX=3661 GN=LOC111486927... [more]
A0A6J1JWL47.2e-21787.65uncharacterized protein LOC111488995 isoform X4 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JYD41.2e-21687.47uncharacterized protein LOC111488995 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G02340.14.1e-4736.61RING/U-box superfamily protein [more]
AT5G08139.11.6e-3836.49RING/U-box superfamily protein [more]
AT5G15820.13.0e-3435.95RING/U-box superfamily protein [more]
AT5G01980.13.5e-3031.60RING/U-box superfamily protein [more]
AT5G60820.11.1e-2829.59RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 354..394
e-value: 2.5E-6
score: 37.0
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 354..395
e-value: 1.1E-10
score: 41.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 354..395
score: 12.076194
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 313..401
e-value: 4.0E-21
score: 76.5
NoneNo IPR availablePANTHERPTHR15710:SF108E3 UBIQUITIN-PROTEIN LIGASE CIP8-LIKE ISOFORM X1coord: 103..415
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 103..415
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 354..395
e-value: 1.40785E-22
score: 88.1436
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 340..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003308.1Tan0003308.1mRNA