Homology
BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match:
Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)
HSP 1 Score: 496.5 bits (1277), Expect = 3.7e-139
Identity = 266/560 (47.50%), Postives = 366/560 (65.36%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
+E SPANF+PLSP+ FL+R++ VY R S+V+G TW +TY RCL+LASAL +
Sbjct: 48 IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+S GD+VAA+APN+P ++ELHFAVPMAG I+CPLNT+LD +TLS+LL E KI+F+D
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167
Query: 121 QYLPI---VLKSLSQNGKFNIKIPALVLIPADLDTPPSEFP-----------DYNRVLEM 180
Q L I L L+++ K K LVLI D S+ DY +
Sbjct: 168 QLLEIAHGALDLLAKSDKTR-KSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227
Query: 181 RFGDF---TSKPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSP 240
+ GD KP E DPISIN TSG+T KG +YSHR AYLNSLAT+F + + P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287
Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI 300
V+LWTV MF CNGWC +W +AA GG NICLR V+ IF ++ +HKVT + G T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347
Query: 301 SE--SSHPKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
+ KPL RV+++ G+ P+ +IL K+ E GFN+S+ YG+TE GP WKP
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407
Query: 361 FDEETVQFEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYK 420
+D +++ + + + +DVKDP++ME+V DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467
Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSK 480
+++AT +AF GDW+ +GD+A+++ G IE+KDR KD+++ GE +S+V VE VL SH
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527
Query: 481 VAEAAVIGKFEVGFGERLCGFVKLKNG-SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPM 532
V EAAV+ + + +G+ CGFVKLK G + EEII FCR HLP +M P+ +VFGD+P
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587
BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match:
M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)
HSP 1 Score: 490.3 bits (1261), Expect = 2.7e-137
Identity = 264/546 (48.35%), Postives = 353/546 (64.65%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME AN PLSP+ FL+R+S Y S+VYG +TW++T+ RCLKLASAL HF
Sbjct: 29 MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+SPGD+VA + NIPE+YELHFAVPMAG I+C LN + D+A +S LL E K+IF++
Sbjct: 89 GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDF-TSKPNGEL 180
Q L +L + +IK P LVL+ S + YN +L DF +P E
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208
Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCF 240
DPISIN TSG+T K +YSHR AYLNS+AT+ G+ T S V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM-GTRS-VYLWSVPMFHCNGWCF 268
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI---SESSHPKPLSRRV 300
W AA G NIC+R V+ AIF+++ LHKVT T+L MI E + PL +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328
Query: 301 DLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-- 360
+++ G+ P +++ ++ E GF +++ YG+TE GPA KP +D EE +
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKAR 388
Query: 361 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
+ + E+DV+DP+SMESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448
Query: 421 TGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFG 480
TGD+ ++H+ G I++KDR KD+V+ GE VSTV VE VL SH V EAAV+ + + +G
Sbjct: 449 TGDLGVKHEDGYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWG 508
Query: 481 ERLCGFVKLKNG--SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREK 532
E C FV LK G + VS ++IIKFCR LP +M P+ VVF +LP STGKIQK+ ++EK
Sbjct: 509 ETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568
BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match:
M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)
HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 257/546 (47.07%), Postives = 350/546 (64.10%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME AN PLSP+ FL+R+S Y S+VYG +TW++T+ RCLKLASAL H
Sbjct: 29 MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+SPGD+VA + N+PE+YELHFAVPMAG I+C LN + D+A +S LL E K+IF++
Sbjct: 89 GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDF-TSKPNGEL 180
Q L +L + +IK P LVL+ S + YN +L DF +P E
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208
Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCF 240
DPISIN TSG+T K +YSHR AYLNS+AT+ G+ T V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTT--SVYLWSVPMFHCNGWCF 268
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI---SESSHPKPLSRRV 300
W AA G NIC+R V+ AIF+++ LHKVT T+L MI E + PL +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328
Query: 301 DLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-- 360
+++ G+ P +++ ++ E GF +++ YG+TE GPA KP +D EE +
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKAR 388
Query: 361 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
+ + E+DV+DP++MESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448
Query: 421 TGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFG 480
+GD+ ++H+ G I++KDR KD+V+ GE +STV VE VL SH V EAAV+ + + +G
Sbjct: 449 SGDLGVKHEDGYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWG 508
Query: 481 ERLCGFVKLKNG--SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREK 532
E C FV LK G + VS ++IIKFCR LP +M P+ VVF +LP STGKIQK+ ++EK
Sbjct: 509 ETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568
BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match:
F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)
HSP 1 Score: 479.2 bits (1232), Expect = 6.2e-134
Identity = 250/547 (45.70%), Postives = 347/547 (63.44%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME SPAN+ PL+P+ FL R++ VY R S+VYG +TW +T RC+++ASAL
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+S GD+V+ +APN+P + ELHF VPMAGA++C LN + D++ +++LL+ K+IF D
Sbjct: 63 GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TS 180
Q+L I + K+P LVLIP L S E +Y V+ M DF
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVI 182
Query: 181 KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFR 240
+P E D IS+N TSG+T KG +YSHR AYLNSLA + + + + SP +LWT MF
Sbjct: 183 RPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFH 242
Query: 241 CNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKP 300
CNGWC +W + A+GG NICLR VT AIF+++ HKVT + G T+L MI + S KP
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302
Query: 301 LSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETV 360
L +V I A P ++ K+ E GF++ + YG+TE GP I WKP +D EE
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362
Query: 361 QFE-----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFS 420
+ + + + EI VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422
Query: 421 GDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKF 480
G W+ +GD+ ++H G IE+KDR+KDI++ GE +S++ VE+ L +H V EAAV+ +
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARP 482
Query: 481 EVGFGERLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAV 530
+ +GE C FVKLK+GSK S EE+I +CR LP +M P +VF DLP STGK+QKF +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 542
BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match:
Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)
HSP 1 Score: 409.5 bits (1051), Expect = 6.0e-113
Identity = 222/556 (39.93%), Postives = 348/556 (62.59%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M++ AN PL+P+ FLKRAS Y R S++YG FTW +TY RC +LA++L+
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+++ D+V+ +APN+P +YE+HF+VPM GA++ P+NT+LDA T++++L+ EPKI+F+D
Sbjct: 61 NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDT---PPSEFPDYNRVLEMRFGDFTS---- 180
++ P++ + L + + +++ ++D+ P S+ DY ++ R G+ T
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180
Query: 181 ---KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATI--FRSGICNTPSPVFLWT 240
+ + E DPIS+N TSG+T KG + SH+ AYL++L++I + GI PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH 300
+ MF CNGW W++AA GG N+C+R VT I+ ++ELH VT + T+ + + E S
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300
Query: 301 --PKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
P S V ++ G+ P ++ KV + GF++ +GYG+TEA GP + W+ +++
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360
Query: 361 ETVQFE-------DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
E Q E +T ++DVK+ ++ESV DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420
Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAA 480
EAF W TGD+ + H G +E+KDR+KDI++ GE +S++ VE VL + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480
Query: 481 VIGKFEVGFGERLCGFVKLKNGSK---VSVEEIIKFCRAHLPEFMVPEKVV-FGDLPMNS 530
V+ +GE C FV LK G + S ++IK+CR ++P FM P+KVV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540
BLAST of Tan0003265 vs. NCBI nr
Match:
XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])
HSP 1 Score: 874.8 bits (2259), Expect = 3.9e-250
Identity = 440/540 (81.48%), Postives = 474/540 (87.78%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ FF SPANF PLSP+ F KRA+AVYGGRPSV+YG ++FTWSETYGRCL LASALVHHF
Sbjct: 42 MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP +L+SLSQ +I+ PALVLIPA DTPPSEF DYNRVLEMRF +FT + N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH VAEAAVI GERLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF REKAKALN NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574
BLAST of Tan0003265 vs. NCBI nr
Match:
XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])
HSP 1 Score: 874.4 bits (2258), Expect = 5.0e-250
Identity = 442/540 (81.85%), Postives = 475/540 (87.96%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ F SPANF PLSPV FLK+++AVY RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 49 MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
HVSPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP VL+SLSQ +I+ PALVLIPA DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH VAEAAV+ GERLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI+KFCR HLPEFM+PE+VVFGDLPMNS GK+QKF REKAKALN +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581
BLAST of Tan0003265 vs. NCBI nr
Match:
KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 872.1 bits (2252), Expect = 2.5e-249
Identity = 441/540 (81.67%), Postives = 472/540 (87.41%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ F SPANF PLSPV F KRA+AVY RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 1 MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP VLKSLSQ +I+ PALVLIPA DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDPMSMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDIVVRPNGEA-VSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGEA VSTV VEAVLMSH VAEAAV+ GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI++FCR HLPEFM+PE+VVFGDLPMNS GK+QKF VREKAK LN +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533
BLAST of Tan0003265 vs. NCBI nr
Match:
KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-248
Identity = 439/540 (81.30%), Postives = 470/540 (87.04%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ F SPANF PLSPV F KRA+AVY RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 1 MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP VLKSLSQ +I+ PALVLIP DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTS 360
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDPMSMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH VAEAAV+ GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI++FCR HLPEFM+PE+VVFGDLPMNS GK+QKF VREKAK LN +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533
BLAST of Tan0003265 vs. NCBI nr
Match:
XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 866.3 bits (2237), Expect = 1.4e-247
Identity = 436/540 (80.74%), Postives = 473/540 (87.59%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ F PANF PLSP+ F +++AVYG RPS++YG ++FTWSETYGRCL LASALVHHF
Sbjct: 1 MDPFSPFPANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHF 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL+PK+IF+DS
Sbjct: 61 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDS 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP VLKSLSQ +++ PALVLIPA DTP SEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELD 180
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT + IF ++ELHKVTLLCGP+TLLKMI ESS + KP LSRR
Sbjct: 241 IWAMAALGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 300
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNF EETVQFEDLITS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITS 360
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH V EAAVI GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI-------GERLCGFV 480
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF VREKAKALN NT
Sbjct: 481 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNNT 533
BLAST of Tan0003265 vs. ExPASy TrEMBL
Match:
A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)
HSP 1 Score: 874.8 bits (2259), Expect = 1.9e-250
Identity = 440/540 (81.48%), Postives = 474/540 (87.78%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ FF SPANF PLSP+ F KRA+AVYGGRPSV+YG ++FTWSETYGRCL LASALVHHF
Sbjct: 42 MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP +L+SLSQ +I+ PALVLIPA DTPPSEF DYNRVLEMRF +FT + N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH VAEAAVI GERLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF REKAKALN NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574
BLAST of Tan0003265 vs. ExPASy TrEMBL
Match:
A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)
HSP 1 Score: 874.4 bits (2258), Expect = 2.4e-250
Identity = 442/540 (81.85%), Postives = 475/540 (87.96%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M+ F SPANF PLSPV FLK+++AVY RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 49 MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
HVSPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
+LP VL+SLSQ +I+ PALVLIPA DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228
Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288
Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS + KP LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408
Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468
Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH VAEAAV+ GERLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528
Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
KLKNGS+ S EEI+KFCR HLPEFM+PE+VVFGDLPMNS GK+QKF REKAKALN +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581
BLAST of Tan0003265 vs. ExPASy TrEMBL
Match:
A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)
HSP 1 Score: 804.7 bits (2077), Expect = 2.4e-229
Identity = 415/541 (76.71%), Postives = 461/541 (85.21%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME F H P NFAPLSPV FLKRAS +YG RPS++YG ++FTWS+TYGRCL +ASALVHHF
Sbjct: 1 MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
H+SP DLV AMAPNIPELYELHFAVPMAG II LNTKLD+ TLSLLLQQL PK+IF+DS
Sbjct: 61 HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNG 180
Q+LPI+LKSL +IK PAL+LIP+D +T PS+F DYN++L MR G +FT +PN
Sbjct: 121 QFLPILLKSLE---NISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180
Query: 181 ELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNG 240
ELDPISIN TSGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNG
Sbjct: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240
Query: 241 WCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPL 300
WCFIW MAALGGCNICLRTVT DAIF +VELH+VTLLCGP+TLLKMI ESS P+ L
Sbjct: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRL 300
Query: 301 SRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEETVQFED 360
SRRVDLIVAGALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D
Sbjct: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDD 360
Query: 361 -LITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTG 420
LITSLEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTG
Sbjct: 361 HLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTG 420
Query: 421 DVAIRHKSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGE 480
DV +RHKSGRIEMKDRAKDIVVR +GE AVSTV VE VLMSH VAE AV+G+
Sbjct: 421 DVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------R 480
Query: 481 RLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKA 530
L GFVKLKNGSK + +EI++FCR HLPEFMVP+ +VFGDLPMNSTGK+QKF +REKAKA
Sbjct: 481 TLYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKA 532
BLAST of Tan0003265 vs. ExPASy TrEMBL
Match:
A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)
HSP 1 Score: 714.9 bits (1844), Expect = 2.5e-202
Identity = 375/475 (78.95%), Postives = 408/475 (85.89%), Query Frame = 0
Query: 71 MAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSL 130
MAPNIPELYELHFAVPMAGAII LNTKLD+ TLSLLLQQL PK+IFIDSQ+LPI+LKSL
Sbjct: 1 MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60
Query: 131 SQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNGELDPISINCT 190
+ +IK PALVLIP+D DTP PSEF DYN+VL MRFG DFT +PN ELDPISIN T
Sbjct: 61 ENS---SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYT 120
Query: 191 SGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCFIWAMAAL 250
SGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180
Query: 251 GGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPLSRRVDLIVAG 310
GGCNICLRTVT DAIF +VELH+VTLLCGP TLLKMI ESS P+ LSRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240
Query: 311 ALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVKD 370
ALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP F+E+ VQFEDLITSLEIDVKD
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKD 300
Query: 371 PMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRIE 430
P+SMESVL DGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV +RHKSGRIE
Sbjct: 301 PISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIE 360
Query: 431 MKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKNGS 490
MKDRAKDIVVR +GE AVSTV VE VLMSH VAEAAV+G+ L GFVKLKN S
Sbjct: 361 MKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGE------RTLYGFVKLKNRS 420
Query: 491 KVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL--NAYN 534
K + +EI++FCR HLPEFM+P+++VFGDLPMNSTGK+QKFA+REK KAL NA N
Sbjct: 421 KENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANN 466
BLAST of Tan0003265 vs. ExPASy TrEMBL
Match:
A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)
HSP 1 Score: 693.3 bits (1788), Expect = 7.7e-196
Identity = 364/471 (77.28%), Postives = 402/471 (85.35%), Query Frame = 0
Query: 71 MAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSL 130
MAPNIPELYELHFAVPMAG II LNTKLD+ TLSLLLQQL PK+IF+DSQ+LPI+LKSL
Sbjct: 1 MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60
Query: 131 SQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNGELDPISINCT 190
+IK PAL+LIP+D +T PS+F DYN++L MR G +FT +PN ELDPISIN T
Sbjct: 61 E---NISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYT 120
Query: 191 SGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCFIWAMAAL 250
SGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180
Query: 251 GGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPLSRRVDLIVAG 310
GGCNICLRTVT DAIF +VELH+VTLLCGP+TLLKMI ESS P+ LSRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240
Query: 311 ALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEETVQFED-LITSLEIDV 370
ALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITSLEIDV
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDV 300
Query: 371 KDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGR 430
KDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGDV +RHKSGR
Sbjct: 301 KDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGR 360
Query: 431 IEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKN 490
IEMKDRAKDIVVR +GE AVSTV VE VLMSH VAE AV+G+ L GFVKLKN
Sbjct: 361 IEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RTLYGFVKLKN 420
Query: 491 GSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL 530
GSK + +EI++FCR HLPEFMVP+ +VFGDLPMNSTGK+QKF +REKAKAL
Sbjct: 421 GSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462
BLAST of Tan0003265 vs. TAIR 10
Match:
AT2G17650.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 496.5 bits (1277), Expect = 2.7e-140
Identity = 266/560 (47.50%), Postives = 366/560 (65.36%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
+E SPANF+PLSP+ FL+R++ VY R S+V+G TW +TY RCL+LASAL +
Sbjct: 48 IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+S GD+VAA+APN+P ++ELHFAVPMAG I+CPLNT+LD +TLS+LL E KI+F+D
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167
Query: 121 QYLPI---VLKSLSQNGKFNIKIPALVLIPADLDTPPSEFP-----------DYNRVLEM 180
Q L I L L+++ K K LVLI D S+ DY +
Sbjct: 168 QLLEIAHGALDLLAKSDKTR-KSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227
Query: 181 RFGDF---TSKPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSP 240
+ GD KP E DPISIN TSG+T KG +YSHR AYLNSLAT+F + + P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287
Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI 300
V+LWTV MF CNGWC +W +AA GG NICLR V+ IF ++ +HKVT + G T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347
Query: 301 SE--SSHPKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
+ KPL RV+++ G+ P+ +IL K+ E GFN+S+ YG+TE GP WKP
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407
Query: 361 FDEETVQFEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYK 420
+D +++ + + + +DVKDP++ME+V DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467
Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSK 480
+++AT +AF GDW+ +GD+A+++ G IE+KDR KD+++ GE +S+V VE VL SH
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527
Query: 481 VAEAAVIGKFEVGFGERLCGFVKLKNG-SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPM 532
V EAAV+ + + +G+ CGFVKLK G + EEII FCR HLP +M P+ +VFGD+P
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587
BLAST of Tan0003265 vs. TAIR 10
Match:
AT1G20560.1 (acyl activating enzyme 1 )
HSP 1 Score: 479.2 bits (1232), Expect = 4.4e-135
Identity = 250/547 (45.70%), Postives = 347/547 (63.44%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME SPAN+ PL+P+ FL R++ VY R S+VYG +TW +T RC+++ASAL
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+S GD+V+ +APN+P + ELHF VPMAGA++C LN + D++ +++LL+ K+IF D
Sbjct: 63 GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TS 180
Q+L I + K+P LVLIP L S E +Y V+ M DF
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVI 182
Query: 181 KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFR 240
+P E D IS+N TSG+T KG +YSHR AYLNSLA + + + + SP +LWT MF
Sbjct: 183 RPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFH 242
Query: 241 CNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKP 300
CNGWC +W + A+GG NICLR VT AIF+++ HKVT + G T+L MI + S KP
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302
Query: 301 LSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETV 360
L +V I A P ++ K+ E GF++ + YG+TE GP I WKP +D EE
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362
Query: 361 QFE-----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFS 420
+ + + + EI VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422
Query: 421 GDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKF 480
G W+ +GD+ ++H G IE+KDR+KDI++ GE +S++ VE+ L +H V EAAV+ +
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARP 482
Query: 481 EVGFGERLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAV 530
+ +GE C FVKLK+GSK S EE+I +CR LP +M P +VF DLP STGK+QKF +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 542
BLAST of Tan0003265 vs. TAIR 10
Match:
AT1G66120.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 409.5 bits (1051), Expect = 4.3e-114
Identity = 222/556 (39.93%), Postives = 348/556 (62.59%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
M++ AN PL+P+ FLKRAS Y R S++YG FTW +TY RC +LA++L+
Sbjct: 1 MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+++ D+V+ +APN+P +YE+HF+VPM GA++ P+NT+LDA T++++L+ EPKI+F+D
Sbjct: 61 NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDT---PPSEFPDYNRVLEMRFGDFTS---- 180
++ P++ + L + + +++ ++D+ P S+ DY ++ R G+ T
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180
Query: 181 ---KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATI--FRSGICNTPSPVFLWT 240
+ + E DPIS+N TSG+T KG + SH+ AYL++L++I + GI PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240
Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH 300
+ MF CNGW W++AA GG N+C+R VT I+ ++ELH VT + T+ + + E S
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300
Query: 301 --PKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
P S V ++ G+ P ++ KV + GF++ +GYG+TEA GP + W+ +++
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360
Query: 361 ETVQFE-------DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
E Q E +T ++DVK+ ++ESV DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420
Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAA 480
EAF W TGD+ + H G +E+KDR+KDI++ GE +S++ VE VL + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480
Query: 481 VIGKFEVGFGERLCGFVKLKNGSK---VSVEEIIKFCRAHLPEFMVPEKVV-FGDLPMNS 530
V+ +GE C FV LK G + S ++IK+CR ++P FM P+KVV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540
BLAST of Tan0003265 vs. TAIR 10
Match:
AT1G20560.2 (acyl activating enzyme 1 )
HSP 1 Score: 402.9 bits (1034), Expect = 4.0e-112
Identity = 214/470 (45.53%), Postives = 295/470 (62.77%), Query Frame = 0
Query: 78 LYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSLSQNGKFN 137
+ ELHF VPMAGA++C LN + D++ +++LL+ K+IF D Q+L I +
Sbjct: 1 MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60
Query: 138 IKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TSKPNGELDPISINCTSGS 197
K+P LVLIP L S E +Y V+ M DF +P E D IS+N TSG+
Sbjct: 61 DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120
Query: 198 TGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCFIWAMAALGGCN 257
T KG +YSHR AYLNSLA + + + + SP +LWT MF CNGWC +W + A+GG N
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFHCNGWCLLWGVTAIGGTN 180
Query: 258 ICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKPLSRRVDLIVAGALPIHE 317
ICLR VT AIF+++ HKVT + G T+L MI + S KPL +V I A P
Sbjct: 181 ICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAH 240
Query: 318 ILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-----DLITSLEID 377
++ K+ E GF++ + YG+TE GP I WKP +D EE + + + + EI
Sbjct: 241 VIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQ 300
Query: 378 VKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRHKSGR 437
VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF G W+ +GD+ ++H G
Sbjct: 301 VKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGY 360
Query: 438 IEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKNG 497
IE+KDR+KDI++ GE +S++ VE+ L +H V EAAV+ + + +GE C FVKLK+G
Sbjct: 361 IELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDG 420
Query: 498 SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL 530
SK S EE+I +CR LP +M P +VF DLP STGK+QKF +R KAKAL
Sbjct: 421 SKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467
BLAST of Tan0003265 vs. TAIR 10
Match:
AT1G76290.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 386.3 bits (991), Expect = 3.9e-107
Identity = 218/544 (40.07%), Postives = 323/544 (59.38%), Query Frame = 0
Query: 1 MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
ME AN+ PLSP+ FL+RA+ V+G R SVVYG +TW +T RC++LASAL
Sbjct: 1 MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASAL-SDL 60
Query: 61 HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
+S D+VAA+APN+P L EL+F PMAGA++C LNT D+ L++ L++ +PK+ F+DS
Sbjct: 61 GLSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDS 120
Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTS-KPNGEL 180
++L + +SLS NI+ L++ + T S++ Y L +F +P E
Sbjct: 121 EFLSVAEESLSLLS--NIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDEC 180
Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICN--TPSPVFLWTVDMFRCNGW 240
DPI++N TSG+T K +YSHR AYLN+ A G+ N P PV+L TV M+ C+GW
Sbjct: 181 DPIALNFTSGTTSTPKCVVYSHRGAYLNATAV----GVMNEMKPMPVYLCTVPMYHCSGW 240
Query: 241 CFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISES--SHPKPLSRR 300
C+IW + A GG +CLR V + IF+ + HKVT G +L MI+ + S K
Sbjct: 241 CYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWT 300
Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF----DEETVQFE- 360
V ++ G+ P E++ K+ + GF + YG +E G W P + +EE+++ +
Sbjct: 301 VQVMSGGSSP-PEVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLKA 360
Query: 361 ----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWY 420
+ +DV DP +M+SV DG+T+ + LRGNT+MSGY+K+ +AT AF G WY
Sbjct: 361 RDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRGGWY 420
Query: 421 RTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGF 480
+ D+ + G I+ KDR++D V+ GE V + +E +L SH V +A V+G+ +
Sbjct: 421 WSRDMGVIDPDGYIQFKDRSQD-VITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDETL 480
Query: 481 GERLCGFVKLKNGSKVSVEEIIKFCRAHLPE---FMVPEKVVFGDLPMNSTGKIQKFAVR 528
GE +C FVKLK G++ EEII+FC+ L M+P+ VVF D+P TGKI+K +R
Sbjct: 481 GESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLR 535
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9SEY5 | 3.7e-139 | 47.50 | Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1 | [more] |
| M4IS92 | 2.7e-137 | 48.35 | Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1 | [more] |
| M4IRL4 | 1.9e-135 | 47.07 | Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1 | [more] |
| F4HUK6 | 6.2e-134 | 45.70 | Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1 | [more] |
| Q9C8D4 | 6.0e-113 | 39.93 | Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_023007650.1 | 3.9e-250 | 81.48 | probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima] | [more] |
| XP_022923197.1 | 5.0e-250 | 81.85 | probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata] | [more] |
| KAG7015844.1 | 2.5e-249 | 81.67 | putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma ... | [more] |
| KAG6577804.1 | 3.6e-248 | 81.30 | putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma ... | [more] |
| XP_023552791.1 | 1.4e-247 | 80.74 | probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1L3K3 | 1.9e-250 | 81.48 | probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
| A0A6J1E5I3 | 2.4e-250 | 81.85 | probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=... | [more] |
| A0A1S3BKP8 | 2.4e-229 | 76.71 | probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 S... | [more] |
| A0A0A0L3L0 | 2.5e-202 | 78.95 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1 | [more] |
| A0A5A7UEI8 | 7.7e-196 | 77.28 | Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... | [more] |
