Tan0002309 (gene) Snake gourd v1

Overview
NameTan0002309
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCalcium-binding EF-hand family protein
LocationLG11: 10422049 .. 10422806 (-)
RNA-Seq ExpressionTan0002309
SyntenyTan0002309
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACATTTATCTTCTTTTGTGTTATATTTCTAAGCCAAGAACCTAAAACATGTCTCCTACTATTAGCTCCAACGACTTGCACAGGATCTTCACCAAGCTCGACAAGAATGGCGATGGCCGCATTTGTCTACACGAGCTCAACTCGCTCCTCGACACGATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTAGAGAAGCCAAGCCTCGACTTCGACGAGTTCTTATTCTTTTACGAATCGATATCGAACCAAAACAATGACGAGTGCAAGGGAGGTGTTGGCGATGATGAGAATGAGATGGTGCATGAGCATGGAGGAAAGGAAGATATTGACATTGTTTATATGGCATTCAAAGTGTTCGACATCAACGACGATGGCTTCATTTCTTCTGAGGAGCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAACAGAAGCGATATCGATTGCAAAAGCATGATTCGAGCTTATGACACTAACTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAATATGATGTTGCTGACAAGTTCTTGATACGTTTTGAAGTGATGTGTTGTATGTTTTTTAGCCTGGTTTTGTATATGTAACATAACACTAGACAATTTTGAGTTAAAAAACTACATGTATTATTAAATTATATGCGAGTTTGTTCATTTAGTTAGAAACATAACCTAAAAAGAAATTTGAAATTTAGAATTTTTGTTTGGGATGAACTATTGTAATATACACACAATACACA

mRNA sequence

CAACATTTATCTTCTTTTGTGTTATATTTCTAAGCCAAGAACCTAAAACATGTCTCCTACTATTAGCTCCAACGACTTGCACAGGATCTTCACCAAGCTCGACAAGAATGGCGATGGCCGCATTTGTCTACACGAGCTCAACTCGCTCCTCGACACGATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTAGAGAAGCCAAGCCTCGACTTCGACGAGTTCTTATTCTTTTACGAATCGATATCGAACCAAAACAATGACGAGTGCAAGGGAGGTGTTGGCGATGATGAGAATGAGATGGTGCATGAGCATGGAGGAAAGGAAGATATTGACATTGTTTATATGGCATTCAAAGTGTTCGACATCAACGACGATGGCTTCATTTCTTCTGAGGAGCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAACAGAAGCGATATCGATTGCAAAAGCATGATTCGAGCTTATGACACTAACTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAATATGATGTTGCTGACAAGTTCTTGATACGTTTTGAAGTGATGTGTTGTATGTTTTTTAGCCTGGTTTTGTATATGTAACATAACACTAGACAATTTTGAGTTAAAAAACTACATGTATTATTAAATTATATGCGAGTTTGTTCATTTAGTTAGAAACATAACCTAAAAAGAAATTTGAAATTTAGAATTTTTGTTTGGGATGAACTATTGTAATATACACACAATACACA

Coding sequence (CDS)

ATGTCTCCTACTATTAGCTCCAACGACTTGCACAGGATCTTCACCAAGCTCGACAAGAATGGCGATGGCCGCATTTGTCTACACGAGCTCAACTCGCTCCTCGACACGATTGGCATCCAACTAACCATGGAGGAGTTGGAGTCTTTCCTAGAGAAGCCAAGCCTCGACTTCGACGAGTTCTTATTCTTTTACGAATCGATATCGAACCAAAACAATGACGAGTGCAAGGGAGGTGTTGGCGATGATGAGAATGAGATGGTGCATGAGCATGGAGGAAAGGAAGATATTGACATTGTTTATATGGCATTCAAAGTGTTCGACATCAACGACGATGGCTTCATTTCTTCTGAGGAGCTCCAAAACGTGTTGTCGAGACTAGAGTTTTGGGACGCAAACAGAAGCGATATCGATTGCAAAAGCATGATTCGAGCTTATGACACTAACTTGGATGGAAAGCTCGATTTCGAGGAGTTTAAGAATATGATGTTGCTGACAAGTTCTTGA

Protein sequence

MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEFLFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS
Homology
[Close]
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 72/161 (44.72%), Postives = 100/161 (62.11%), Query Frame = 0 

Query: 5   ISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIG-IQLTMEELESFLEKPSLDFDEFL-F 64
           I++NDL R+F  LDKN DG + L EL  +LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  FYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNV 124
           +Y+++ +    +    V  D +E +              AF VFD+N DG+IS+EEL++V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEAIAR------------AFNVFDVNGDGYISAEELRDV 125

Query: 125 LSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMML 164
           L RL F +  ++  DC  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLGFEEEAKA-WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.9e-19
Identity = 64/168 (38.10%), Postives = 94/168 (55.95%), Query Frame = 0 

Query: 2   SPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLE------KPSL 61
           SP++++  L RIF   DKNGDG I + EL+  L  +G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDEFLFFYESISNQNNDECKGGV-GDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFI 121
           +FD+F   ++++    +D   GG  G  ENE       + + D+   AFKVFD N DGFI
Sbjct: 82  NFDDFSSLHKTL----DDSFFGGACGGGENEDDPSSAAENESDLA-EAFKVFDENGDGFI 141

Query: 122 SSEELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           S+ ELQ VL +L   +    +   + MI + D N DG++DF EFKNMM
Sbjct: 142 SARELQTVLKKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.3e-17
Identity = 61/166 (36.75%), Postives = 86/166 (51.81%), Query Frame = 0 

Query: 5   ISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEK------PSLDFD 64
           + S +L ++F   DKNGDGRI   EL       GI +  +EL++ ++K        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 65  EFLFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYM--AFKVFDINDDGFISS 124
           EF   Y SI     D+  G         +   GG  D +   M  AF VFD N DGFI+ 
Sbjct: 61  EFGLLYRSIL---GDDAAGRAPRTAAAAIGGEGGAPDDEDEGMREAFNVFDQNGDGFITV 120

Query: 125 EELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           +EL++VLS L       +D DC+ MI   D + DG++DF+EFK MM
Sbjct: 121 DELRSVLSSLGLKHGRTAD-DCRRMISMVDADGDGRVDFKEFKQMM 162

[Close]
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 5.6e-17
Identity = 61/169 (36.09%), Postives = 100/169 (59.17%), Query Frame = 0 

Query: 2   SPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLE------KPSL 61
           S ++++  L RIF   DKN DG I + EL+  L+ +G++  + ELES ++         L
Sbjct: 33  SESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGL 92

Query: 62  DFDEFLFFYESISNQNNDECKGGVGDDENE--MVHEHGGKEDIDIVYMAFKVFDINDDGF 121
            F++F+  ++S++  ++    GG  +D+NE  M      +E+ D  +  FKVFD + DG+
Sbjct: 93  QFEDFISLHQSLN--DSYFAYGGEDEDDNEEDMRKSILSQEEAD-SFGGFKVFDEDGDGY 152

Query: 122 ISSEELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           IS+ ELQ VL +L F + +  D   + MI + D+N DG++DF EFK+MM
Sbjct: 153 ISARELQMVLGKLGFSEGSEID-RVEKMIVSVDSNRDGRVDFFEFKDMM 197

[Close]
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.1e-16
Identity = 57/160 (35.62%), Postives = 83/160 (51.88%), Query Frame = 0 

Query: 9   DLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKNGDG+I  +EL     ++GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 69  FYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNV 128
            Y+                   EMV E   KE+ + +  AF+VFD N DGFI+ EEL++V
Sbjct: 65  LYQ-------------------EMVEE---KEEEEDMREAFRVFDQNGDGFITDEELRSV 124

Query: 129 LSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           L+ +      R+  DCK MI   D + DG ++F+EFK MM
Sbjct: 125 LASMGL-KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Tan0002309 vs. NCBI nr
Match: XP_022141411.1 (probable calcium-binding protein CML44 [Momordica charantia])

HSP 1 Score: 258.8 bits (660), Expect = 3.1e-65
Identity = 130/167 (77.84%), Postives = 146/167 (87.43%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP I SNDL +IF KLDKN DG +CL EL+ LLD IGIQLTMEELES LEKPSLDF+EF
Sbjct: 1   MSP-ICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS +N  EC GGVGDDENEM+  H G+ED+DIVYMAFKVFD+NDDGFIS EEL+
Sbjct: 61  LFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELR 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
           +VL RLE WD+NRSD +C++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 SVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]
BLAST of Tan0002309 vs. NCBI nr
Match: XP_022989807.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 249.2 bits (635), Expect = 2.5e-62
Identity = 127/167 (76.05%), Postives = 139/167 (83.23%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP I+ NDL RIF KLDKN DG ICLHEL+ LLDTIG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS QN  ECKGGV D        +  ++D+DIVYMAFKVFD+NDDGFIS EELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREDDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
           +VLSRLE WD NRS IDCKSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 HVLSRLEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]
BLAST of Tan0002309 vs. NCBI nr
Match: KAG6602129.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249.2 bits (635), Expect = 2.5e-62
Identity = 128/167 (76.65%), Postives = 137/167 (82.04%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP IS NDL RIF KLDKN DG ICLHELN LLDTIG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS QN  ECKGGV D        +  +ED+DIVYMAFKVFD+NDDGFIS EELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREEDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
            VLSRLE WD N +D DCKSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 LVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]
BLAST of Tan0002309 vs. NCBI nr
Match: XP_022993348.1 (probable calcium-binding protein CML44 [Cucurbita maxima])

HSP 1 Score: 248.1 bits (632), Expect = 5.5e-62
Identity = 124/166 (74.70%), Postives = 139/166 (83.73%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           M+P ISSNDL RIF +LDKNGDG IC+ EL  LLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           L FYES+S QN  ECKGG+ D+  +        ED++IVYMAFKVFD+N DGFIS EEL+
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHED------EDEDVEIVYMAFKVFDVNGDGFISCEELE 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 167
           NVLSRLE WDANRS++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 121 NVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]
BLAST of Tan0002309 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 248.1 bits (632), Expect = 5.5e-62
Identity = 127/167 (76.05%), Postives = 140/167 (83.83%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           M+  ISSNDL RIF +LDKN DG IC+ EL  LLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGG-KEDIDIVYMAFKVFDINDDGFISSEEL 120
           LFFYESIS QN  ECKGG       +V EHG  +ED++IVYMAFKVFD+N DGFIS EEL
Sbjct: 61  LFFYESISKQNKGECKGG-------LVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEEL 120

Query: 121 QNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 167
           +NVLSRLE WDANRS++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 121 ENVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match: A0A6J1CJ36 (probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC111011820 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-65
Identity = 130/167 (77.84%), Postives = 146/167 (87.43%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP I SNDL +IF KLDKN DG +CL EL+ LLD IGIQLTMEELES LEKPSLDF+EF
Sbjct: 1   MSP-ICSNDLLKIFNKLDKNCDGLVCLQELHWLLDRIGIQLTMEELESSLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS +N  EC GGVGDDENEM+  H G+ED+DIVYMAFKVFD+NDDGFIS EEL+
Sbjct: 61  LFFYESISKRNKGECNGGVGDDENEMLDGHEGEEDVDIVYMAFKVFDMNDDGFISCEELR 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
           +VL RLE WD+NRSD +C++MIRAYDTNLDGKLDFEEFKNMMLLT+S
Sbjct: 121 SVLLRLELWDSNRSDSECRNMIRAYDTNLDGKLDFEEFKNMMLLTTS 166

[Close]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match: A0A6J1JGU4 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111486883 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.2e-62
Identity = 127/167 (76.05%), Postives = 139/167 (83.23%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP I+ NDL RIF KLDKN DG ICLHEL+ LLDTIG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MSPIININDLLRIFNKLDKNRDGLICLHELSWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS QN  ECKGGV D        +  ++D+DIVYMAFKVFD+NDDGFIS EELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREDDVDIVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
           +VLSRLE WD NRS IDCKSMIRAYDTNLDG LDFEEFKNMM LTSS
Sbjct: 121 HVLSRLEIWDVNRSGIDCKSMIRAYDTNLDGMLDFEEFKNMMSLTSS 160

[Close]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.7e-62
Identity = 124/166 (74.70%), Postives = 139/166 (83.73%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           M+P ISSNDL RIF +LDKNGDG IC+ EL  LLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MAPPISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           L FYES+S QN  ECKGG+ D+  +        ED++IVYMAFKVFD+N DGFIS EEL+
Sbjct: 61  LIFYESMSKQNKGECKGGLVDEHED------EDEDVEIVYMAFKVFDVNGDGFISCEELE 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 167
           NVLSRLE WDANRS++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 121 NVLSRLELWDANRSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 6.0e-62
Identity = 126/167 (75.45%), Postives = 140/167 (83.83%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           M+  ISSNDL RIF +LDKN DG IC+ EL  LLDTIGIQLTMEELESFLEKPSLDFDEF
Sbjct: 1   MALPISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGG-KEDIDIVYMAFKVFDINDDGFISSEEL 120
           LFFYESIS QN  ECKGG       +V EHG  +ED++IVYMAFKVFD+N DGFIS EEL
Sbjct: 61  LFFYESISKQNKGECKGG-------LVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEEL 120

Query: 121 QNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTS 167
           +NVLSRLE WDAN+S++DC+SMIRAYDTNLDGKLDFEEF NMML T+
Sbjct: 121 ENVLSRLELWDANKSEVDCRSMIRAYDTNLDGKLDFEEFMNMMLFTN 160

[Close]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match: A0A6J1H868 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111460440 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 7.8e-62
Identity = 126/167 (75.45%), Postives = 136/167 (81.44%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSLDFDEF 60
           MSP IS NDL RIF KLDKN DG ICLHELN LLDTIG++LTME+L SFLEKPSLDF+EF
Sbjct: 1   MSPIISINDLLRIFNKLDKNRDGLICLHELNWLLDTIGVELTMEDLHSFLEKPSLDFNEF 60

Query: 61  LFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQ 120
           LFFYESIS QN  ECKGGV D        +  +ED+D+VYMAFKVFD+NDDGFIS EELQ
Sbjct: 61  LFFYESISKQNKGECKGGVSD-------VNAREEDVDVVYMAFKVFDMNDDGFISCEELQ 120

Query: 121 NVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMMLLTSS 168
            VLSRLE WD N +D DCKSMIRAYDTNLDG LDFEEFKNMM L SS
Sbjct: 121 LVLSRLELWDVNTTDTDCKSMIRAYDTNLDGMLDFEEFKNMMSLRSS 160

[Close]
BLAST of Tan0002309 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.5 bits (293), Expect = 1.0e-26
Identity = 72/161 (44.72%), Postives = 100/161 (62.11%), Query Frame = 0 

Query: 5   ISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIG-IQLTMEELESFLEKPSLDFDEFL-F 64
           I++NDL R+F  LDKN DG + L EL  +LD +G  + T +ELE  + K SLD DEFL F
Sbjct: 6   ITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFLRF 65

Query: 65  FYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNV 124
           +Y+++ +    +    V  D +E +              AF VFD+N DG+IS+EEL++V
Sbjct: 66  YYDAVLDSKGSKKNIDVVADNDEAIAR------------AFNVFDVNGDGYISAEELRDV 125

Query: 125 LSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMML 164
           L RL F +  ++  DC  MIR +D NLDG +DFEEFKNM+L
Sbjct: 126 LERLGFEEEAKA-WDCGRMIRVHDKNLDGFVDFEEFKNMIL 153

[Close]
BLAST of Tan0002309 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 95.1 bits (235), Expect = 5.6e-20
Identity = 64/168 (38.10%), Postives = 94/168 (55.95%), Query Frame = 0 

Query: 2   SPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLE------KPSL 61
           SP++++  L RIF   DKNGDG I + EL+  L  +G+   + +L+S +E         L
Sbjct: 22  SPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGL 81

Query: 62  DFDEFLFFYESISNQNNDECKGGV-GDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFI 121
           +FD+F   ++++    +D   GG  G  ENE       + + D+   AFKVFD N DGFI
Sbjct: 82  NFDDFSSLHKTL----DDSFFGGACGGGENEDDPSSAAENESDLA-EAFKVFDENGDGFI 141

Query: 122 SSEELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           S+ ELQ VL +L   +    +   + MI + D N DG++DF EFKNMM
Sbjct: 142 SARELQTVLKKLGLPEGGEME-RVEKMIVSVDRNQDGRVDFFEFKNMM 183

[Close]
BLAST of Tan0002309 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-17
Identity = 57/160 (35.62%), Postives = 83/160 (51.88%), Query Frame = 0 

Query: 9   DLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEK------PSLDFDEFLF 68
           +L R+F   DKNGDG+I  +EL     ++GI +   E+   + K       ++D DEF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 69  FYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNV 128
            Y+                   EMV E   KE+ + +  AF+VFD N DGFI+ EEL++V
Sbjct: 65  LYQ-------------------EMVEE---KEEEEDMREAFRVFDQNGDGFITDEELRSV 124

Query: 129 LSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           L+ +      R+  DCK MI   D + DG ++F+EFK MM
Sbjct: 125 LASMGL-KQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]
BLAST of Tan0002309 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 86.3 bits (212), Expect = 2.6e-17
Identity = 61/168 (36.31%), Postives = 87/168 (51.79%), Query Frame = 0 

Query: 1   MSPTISSNDLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEK------PS 60
           M PT    +L R+F   DKNGDG I   EL+  L ++GI +  +EL   +EK        
Sbjct: 1   MDPT----ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGC 60

Query: 61  LDFDEFLFFYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFI 120
           +D DEF   Y++I ++          D+E E + E            AF VFD N DGFI
Sbjct: 61  VDIDEFGELYKTIMDEE---------DEEEEDMKE------------AFNVFDQNGDGFI 120

Query: 121 SSEELQNVLSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           + +EL+ VLS L        D DCK MI+  D + DG+++++EF+ MM
Sbjct: 121 TVDELKAVLSSLGLKQGKTLD-DCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]
BLAST of Tan0002309 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 85.9 bits (211), Expect = 3.4e-17
Identity = 60/160 (37.50%), Postives = 83/160 (51.88%), Query Frame = 0 

Query: 9   DLHRIFTKLDKNGDGRICLHELNSLLDTIGIQLTMEELESFLEKPSL------DFDEFLF 68
           +L RIF   D+NGDG+I   ELN  L+ +GI +  ++L   +EK  L      D +EF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 69  FYESISNQNNDECKGGVGDDENEMVHEHGGKEDIDIVYMAFKVFDINDDGFISSEELQNV 128
            Y++I  +           DE E + E            AF VFD N DGFI+ EEL++V
Sbjct: 65  LYQTIMEER----------DEEEDMRE------------AFNVFDQNRDGFITVEELRSV 124

Query: 129 LSRLEFWDANRSDIDCKSMIRAYDTNLDGKLDFEEFKNMM 163
           L+ L      R+  DCK MI   D + DG ++F+EFK MM
Sbjct: 125 LASLGL-KQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:
BLAST of Tan0002309 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LPK51.5e-2544.72Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG97.9e-1938.10Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q2QVI13.3e-1736.75Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P431875.6e-1736.09Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Q9SU002.1e-1635.63Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1[more]
BLAST of Tan0002309 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022141411.13.1e-6577.84probable calcium-binding protein CML44 [Momordica charantia][more]
XP_022989807.12.5e-6276.05probable calcium-binding protein CML44 [Cucurbita maxima][more]
KAG6602129.12.5e-6276.65putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022993348.15.5e-6274.70probable calcium-binding protein CML44 [Cucurbita maxima][more]
XP_023551499.15.5e-6276.05probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
BLAST of Tan0002309 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CJ361.5e-6577.84probable calcium-binding protein CML44 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1JGU41.2e-6276.05probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114868... [more]
A0A6J1K1Y72.7e-6274.70probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893... [more]
A0A6J1FLU56.0e-6275.45probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A6J1H8687.8e-6275.45probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
BLAST of Tan0002309 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G21550.11.0e-2644.72Calcium-binding EF-hand family protein [more]
AT4G20780.15.6e-2038.10calmodulin like 42 [more]
AT4G12860.11.5e-1735.63EF hand calcium-binding protein family [more]
AT1G05990.12.6e-1736.31EF hand calcium-binding protein family [more]
AT3G07490.13.4e-1737.50ARF-GAP domain 11 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 137..165
e-value: 0.0015
score: 27.8
coord: 98..126
e-value: 0.001
score: 28.3
coord: 9..37
e-value: 0.0064
score: 25.7
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 13..33
e-value: 4.8E-5
score: 22.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 100..162
e-value: 4.8E-9
score: 36.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 133..167
score: 11.472807
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 94..129
score: 12.811786
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 5..40
score: 12.086506
IPR002048EF-hand domainCDDcd00051EFhcoord: 102..162
e-value: 1.99237E-12
score: 57.1725
NoneNo IPR availableGENE3D1.10.238.10coord: 5..65
e-value: 1.4E-10
score: 43.1
NoneNo IPR availableGENE3D1.10.238.10coord: 68..167
e-value: 2.0E-17
score: 65.0
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 5..167
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 5..167
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 18..30
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 107..119
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 9..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002309.1Tan0002309.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding