Tan0002009 (gene) Snake gourd v1

Overview


Name	Tan0002009
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding protein CML22
Location	LG10: 4148061 .. 4151178 (-)
RNA-Seq Expression	Tan0002009
Synteny	Tan0002009

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGCTTGCTCTGTTTTCAAAGAAACCCATAATGGCATCTCCACTTTGGACCAATGCGCAACTCAATTCCATTTTTCTTCTCTCTTTTGCTTTTTTGTTTCCTGTATACTTTTTTTTTCCTCCGCTGAATTTTAGATGAGCTTTCTTCTCTGTGTTTCCATGGAAGTTTCACCTGGTGAGTACGACTTTCTTTTTTTCTTGCAGTCCTTGTCTTCTCTTTTTGGCTAGTTTCTTGTTCTTGCTACTCGATGCTCATTCATTCATTGCTGAGGAAGAACTGGAGATTTTTGTTTTGCTGTACTTGCGCACAAAAGAATTTAATCTGTTCGTCGTTTTGGAATCTGTGTTGTGTGAAAATTTCGATAGAAGTTCCTTGGTTTGTGGTGTGTTGAAATAGAATGCTTGGCTCGTTGAATGTTGCTCGTCGTTTGCGTCGATTTTGCTGTTAATTGTGCCGATTGACGGTGCAAATTAAGGGTTATCCTCGATATTCAATAGCCATTTTGAATGATTGCTTTGTATTTACGCTAAGTTAATCTAAGAAGTTGGGATGAACATAAGTATCTGGTTGAAACCTCAGACTGAAATTGCATGCCAGGGCTGTTCACAACTGAAAGGGCAAACGTCTGAGGGCGAGAAAGATGCAAAAGGATAGATAAGTAGAAATTTGGAAAAAAGGCGATTTACAAGCGATGTAATTTTAACCAAAAGTTGTGTAGTCTAGAAATTATAGGAAATTCTTGTGTCTGAGGCTTTGGGAAAGGATGGATAACTGGAAATTGGTTTTTCTTGCTAGAATTTATTGATGTGATGCTCATGTTACTGCTTGGCAATTGGAAGAATCAACTCTTTTTAGTTTTTCTTTTTCTTTATTAAGCAATAACCAGGAAGAAACTTTGGAGCGTATCCTCCAAAGTACTTTTCCACGTTTCAAGTGGAAGCAAAGTAAATGGGGTTCATTAGAGAGGACAATACTTCAGAAATGCTTGCATATATAGAGCATTTTCTTTCCATTTTCATCGACAAGCAACGATAAACATTGCAACTTGTATTATTCTTACGACGCCCGGTTCCTTGCATCCCTAACTGCTTAATTTCTACTTTCATGTCATTTTTGGCTGTTTGATTGCTTCTGGGATTGCTATCTTGCTCATAGGATACACTGATATTGTTTTCATTTGACTTGTCAGGCACACATTGATTTTCTCTATCTTATGTTACTAAAGGAACATTTCATTCCTGCCCTTATGTCAAAAGTTGGAAGCATGTTTTTCTGCTGCAATTCATCAAATAAATACAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCAATAAATGGCATAATCTTGAGATTTCCCCAATTCAAAGAAGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGGTGAGTTTCATTTTAGCTGCATCACTCTTTTTGAGTTTCTCCTGCTCAAGATTTTCTTACCATACTCTCTCTATCAGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAGAAATGCTTGCAACAGCTGCAAATGCATATGACCGAGGAGGAAGTCAAGGATCTTTTCCAGTCTTGTGACATTGATGGAAGTGCTGGAATACAGTTCAACGAGTTTATCGTTCTTCTATGTCTCATTTATCTCCTCAAGGACCAGAAATCGCCAACCGTAAGCATAAACAAACTTTTATATCTCCATTTTCATTCAGTTTTCAATCTGTTTATGTTTATCATTTTGGACATGAACATTCTGGAGGTTTGTTACAGAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGACACAATCATCCAAGCTTTCATATTTCTTGATAAAAATGGCGATGGCAAGCTCAACAAGAAAGAAATGGTCAAAGCTCTGAATGAGGCTTCTCCATGCGAGAGATCCCCTGCACGCATTATACAAACTAGATTCAGTATTGCTTCCTTTACTAAAGATACTCCATATTGGTTTTTGTTTAATGAATCGTCTAGAAAGTTCAAATATGTCGAACATATCTGATCAAACATGGTGTCAAATACACATTCATACGAGCTAAGTAGTAGATTGACACATTAAGGTCCCATTTGATAACTTTTTTGTTTTGTTTTTGTTTTTGTTTTTGTTTTGAAAATTAAGTTTATAAACGCTATTTTCACCGATGAAATTCTTTCTTTTGTTATCTACGTTTAAAATGTTTTCAAATTCAAGCCCAAATTTGAAAATTAAAAAAAGTTTCTAAAAACTTGTTTTTATTTTTTAAATTAATTTGGCAAATAATTCAAACATTTATGAAATAAAGGTGAAATCCAAAAAAAAAAAAAAAAAATGGTGAGTCAACAAGCCCAATTTTCAAAAACAGAAAATTAAAAACGAAACCTCCCATTTGATAACCATTTGGTCTTTTGTTTTTGAAAATTGTGCTTGTTTTCTATTATTTCTCTAATGGATTTTATTTTTCTTAAGAAAATATTTGAATTCTTAGTTAAAGTTCAAAAACAAGAACAAATTTTTTAGAACTACTTTTTTTTTCTTGGCTTGGTTTTGAAAACATATATAAAAAAAAAATAGATAACAAAACAAGGTAACTCATAGGTGAGAGTAGTATATAGAGGCTTAATTTTCAAAAATCAAATGATTATCAAATGGGTTTAAACTTTCATCTATCTGAACCGTTCTGCTTTTTTATTATATATTGATTTCCCCACTTTTCTTAATGGATGCAGAGGAAATGGACTGGAACAACAGTGGGAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACAGATGATGACCTCTCTCTCACATGAAGTTGTTAAAACAGGACAGAGGACATCAAGACATCATATAGACATGAAGACAATGGCTTAAAAAATAAGGTCAGACTGATAATGTCATTCAAAACTGAACACTTGCTGATGATTATAGCGAGATACTACTCTTGTTTGTTTATTTCATTTGACTAATTGTAATGTTCGAACGACATTGTGAATTAGATTACATAATACTATTCTTATCGTCATTGTTGTTACT

mRNA sequence

GAGCTTGCTCTGTTTTCAAAGAAACCCATAATGGCATCTCCACTTTGGACCAATGCGCAACTCAATTCCATTTTTCTTCTCTCTTTTGCTTTTTTGTTTCCTGTATACTTTTTTTTTCCTCCGCTGAATTTTAGATGAGCTTTCTTCTCTGTGTTTCCATGGAAGTTTCACCTGTTGGAAGCATGTTTTTCTGCTGCAATTCATCAAATAAATACAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCAATAAATGGCATAATCTTGAGATTTCCCCAATTCAAAGAAGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAGAAATGCTTGCAACAGCTGCAAATGCATATGACCGAGGAGGAAGTCAAGGATCTTTTCCAGTCTTGTGACATTGATGGAAGTGCTGGAATACAGTTCAACGAGTTTATCGTTCTTCTATGTCTCATTTATCTCCTCAAGGACCAGAAATCGCCAACCAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGACACAATCATCCAAGCTTTCATATTTCTTGATAAAAATGGCGATGGCAAGCTCAACAAGAAAGAAATGGTCAAAGCTCTGAATGAGGCTTCTCCATGCGAGAGATCCCCTGCACGCATTATACAAACTAGATTCAAGGAAATGGACTGGAACAACAGTGGGAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACAGATGATGACCTCTCTCTCACATGAAGTTGTTAAAACAGGACAGAGGACATCAAGACATCATATAGACATGAAGACAATGGCTTAAAAAATAAGGTCAGACTGATAATGTCATTCAAAACTGAACACTTGCTGATGATTATAGCGAGATACTACTCTTGTTTGTTTATTTCATTTGACTAATTGTAATGTTCGAACGACATTGTGAATTAGATTACATAATACTATTCTTATCGTCATTGTTGTTACT

Coding sequence (CDS)

ATGAGCTTTCTTCTCTGTGTTTCCATGGAAGTTTCACCTGTTGGAAGCATGTTTTTCTGCTGCAATTCATCAAATAAATACAAGAGATTGGATGCAAAGCTTGGAAGAAAAATGCTTGAAGTCAAAAGAAATTTAGCTGGACATGACAATTTCAAGTCAATAAATGGCATAATCTTGAGATTTCCCCAATTCAAAGAAGGATTACAAAACATCAGAGGTGTATTTGAACAGTATGATGAAGATTCCAATGGAAGCATTGATCGCGAAGAACTCAAGAAATGCTTGCAACAGCTGCAAATGCATATGACCGAGGAGGAAGTCAAGGATCTTTTCCAGTCTTGTGACATTGATGGAAGTGCTGGAATACAGTTCAACGAGTTTATCGTTCTTCTATGTCTCATTTATCTCCTCAAGGACCAGAAATCGCCAACCAAATCAAAGCTGGGTTCACCTCAACTTGAAGCAACTTTTGACACAATCATCCAAGCTTTCATATTTCTTGATAAAAATGGCGATGGCAAGCTCAACAAGAAAGAAATGGTCAAAGCTCTGAATGAGGCTTCTCCATGCGAGAGATCCCCTGCACGCATTATACAAACTAGATTCAAGGAAATGGACTGGAACAACAGTGGGAAGGTCAATTTCAGGGAGTTTCTATTTGGTTTCATTAACTGGGTTGGAATTGACACAGATGATGACCTCTCTCTCACATGA

Protein sequence

MSFLLCVSMEVSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT

Homology

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BLAST of Tan0002009 vs. ExPASy Swiss-Prot
Match: Q9LRN6 (Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 2.4e-66
Identity = 127/224 (56.70%), Postives = 166/224 (74.11%), Query Frame = 0


Query: 17  MFFCC-NSSN--KYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQNIRG 76
           M  CC NS N  KY  LDAKL RKM+E +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 77  VFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCL 136
           VFE YD D+NG+ID EELKKCL++L++ +++EEVK L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 137 IYLL--KDQKSPTKSKLGSPQL-EATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEASP 196
           IYLL     +S T+S+   P+L E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 197 CERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDD 234
            ERSP+ +   RF+EMDW   GKV FREFLF F++WVG+D  DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

[Close]

BLAST of Tan0002009 vs. ExPASy Swiss-Prot
Match: Q52K82 (Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 PE=2 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 6.7e-56
Identity = 102/206 (49.51%), Postives = 150/206 (72.82%), Query Frame = 0


Query: 31  DAKLGRKMLE-VKRNLAGHDNFKSINGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 90
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 91  ELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCLIYLLKDQKSPTKSK-- 150
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 151 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARIIQTRFKEMDW 210
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRFEEMDW 197

Query: 211 NNSGKVNFREFLFGFINWVGIDTDDD 234
           + +G VNF+EFLF F  WVGID +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

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BLAST of Tan0002009 vs. ExPASy Swiss-Prot
Match: P41210 (Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-12
Identity = 46/151 (30.46%), Postives = 76/151 (50.33%), Query Frame = 0


Query: 69  QNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFI 128
           Q I+  FE +D D +G+ID +EL   ++ L   MTEE++  +    D DGS  I F+EF 
Sbjct: 25  QEIKEAFELFDTDGSGTIDAKELNVAMRALGFEMTEEQINQMIADVDKDGSGAIDFDEF- 84

Query: 129 VLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEAS 188
              C +   K  +  TK +L            ++AF  +D++ +GK++ +++ +   E  
Sbjct: 85  ---CHMMTAKIGERDTKEEL------------MKAFRIIDQDNNGKISPEDIQRIAKELG 144

Query: 189 PCERSPARIIQTRFKEMDWNNSGKVNFREFL 220
             E    + IQ   +E D +  G+VN  EFL
Sbjct: 145 --ENFTVKDIQDMIEEADRDRDGEVNVEEFL 157

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BLAST of Tan0002009 vs. ExPASy Swiss-Prot
Match: Q9ZSA3 (Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=3 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 3.5e-12
Identity = 46/154 (29.87%), Postives = 80/154 (51.95%), Query Frame = 0


Query: 65  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQF 124
           +E ++ ++ +FE  D D +GSI  EELK  L +    ++E EVK L ++ D+DG+  I +
Sbjct: 345 EEEIKGLKTMFENMDMDKSGSITYEELKMGLNRHGSKLSETEVKQLMEAADVDGNGTIDY 404

Query: 125 NEFIVLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL 184
            EFI                 + +   +LE   + + +AF + DK+G G + K+E+  A+
Sbjct: 405 IEFI----------------SATMHRHRLERD-EHLYKAFQYFDKDGSGHITKEEVEIAM 464

Query: 185 NEASPCERSPARIIQTRFKEMDWNNSGKVNFREF 219
            E    + + A+ +     E D NN GK+++ EF
Sbjct: 465 KEHGMGDEANAKDL---ISEFDKNNDGKIDYEEF 478

[Close]

BLAST of Tan0002009 vs. ExPASy Swiss-Prot
Match: P02588 (Troponin C, skeletal muscle OS=Gallus gallus OX=9031 GN=TNNC2 PE=1 SV=3)

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-11
Identity = 43/155 (27.74%), Postives = 74/155 (47.74%), Query Frame = 0


Query: 65  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQF 124
           +E +   +  F+ +D D  G I  +EL   ++ L  + T+EE+  + +  D DGS  I F
Sbjct: 17  EEMIAEFKAAFDMFDADGGGDISTKELGTVMRMLGQNPTKEELDAIIEEVDEDGSGTIDF 76

Query: 125 NEFIVLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKAL 184
            EF+V+     +++  K   K K        + + +   F   DKN DG ++ +E+ + L
Sbjct: 77  EEFLVM-----MVRQMKEDAKGK--------SEEELANCFRIFDKNADGFIDIEELGEIL 136

Query: 185 NEASPCERSPARIIQTRFKEMDWNNSGKVNFREFL 220
                 E      I+   K+ D NN G+++F EFL
Sbjct: 137 RATG--EHVTEEDIEDLMKDSDKNNDGRIDFDEFL 156

[Close]

BLAST of Tan0002009 vs. NCBI nr
Match: XP_038892181.1 (probable calcium-binding protein CML22 isoform X1 [Benincasa hispida])

HSP 1 Score: 434.5 bits (1116), Expect = 5.9e-118
Identity = 211/228 (92.54%), Postives = 222/228 (97.37%), Query Frame = 0


Query: 9   MEVSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGL 68
           MEVSPVGS+FFCC+SSNKY RLDAKLGRKM+EVKRNLAGHDNFKS+NGIILRFPQFKE L
Sbjct: 1   MEVSPVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEVL 60

Query: 69  QNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFI 128
           QNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGSAGIQFNEFI
Sbjct: 61  QNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFYSCDIDGSAGIQFNEFI 120

Query: 129 VLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEAS 188
           VLLCLIYLLKD+ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEAS
Sbjct: 121 VLLCLIYLLKDEHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEAS 180

Query: 189 PCERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 237
           P ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVGIDTD++LSL
Sbjct: 181 PYERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDEELSL 228

[Close]

BLAST of Tan0002009 vs. NCBI nr
Match: XP_011650876.1 (probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probable calcium-binding protein CML22 [Cucumis sativus] >KGN56734.1 hypothetical protein Csa_010293 [Cucumis sativus])

HSP 1 Score: 426.8 bits (1096), Expect = 1.2e-115
Identity = 208/227 (91.63%), Postives = 218/227 (96.04%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VGS+FFCC+SSNKY RLDAKLGRKM+EVKRN AGHDNFKS+NGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKD+ S TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 238
           ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDDLS T
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

[Close]

BLAST of Tan0002009 vs. NCBI nr
Match: XP_038892189.1 (probable calcium-binding protein CML22 isoform X2 [Benincasa hispida] >XP_038892198.1 probable calcium-binding protein CML22 isoform X2 [Benincasa hispida])

HSP 1 Score: 426.0 bits (1094), Expect = 2.1e-115
Identity = 207/226 (91.59%), Postives = 219/226 (96.90%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VGS+FFCC+SSNKY RLDAKLGRKM+EVKRNLAGHDNFKS+NGIILRFPQFKE LQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFKEVLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFYSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKD+ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP 
Sbjct: 121 LCLIYLLKDEHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 237
           ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVGIDTD++LSL
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDEELSL 226

[Close]

BLAST of Tan0002009 vs. NCBI nr
Match: XP_022924247.1 (probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924248.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924249.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_022924250.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_023526231.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526232.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526233.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo] >XP_023526234.1 probable calcium-binding protein CML22 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 425.2 bits (1092), Expect = 3.6e-115
Identity = 205/226 (90.71%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VG+MF CC+SSNKY R+D KLGRKM+EVKRNLAGHDNFKS+NGIILRFPQF+EGLQN
Sbjct: 1   MSKVGNMFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSSGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKDQ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP 
Sbjct: 121 LCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPF 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 237
           ERSPARI +TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL
Sbjct: 181 ERSPARITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 226

[Close]

BLAST of Tan0002009 vs. NCBI nr
Match: XP_022980390.1 (probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980391.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980392.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_022980393.1 probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima])

HSP 1 Score: 424.5 bits (1090), Expect = 6.1e-115
Identity = 205/227 (90.31%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VG+MF CC+SSNKY R+D KLGRKM+EVKRNLAGHDNFKS+NGIILRFPQF+EGLQN
Sbjct: 1   MSKVGNMFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVF QYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFAQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKDQ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP 
Sbjct: 121 LCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPF 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 238
           ERSPARI +TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL+
Sbjct: 181 ERSPARITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLS 227

[Close]

BLAST of Tan0002009 vs. ExPASy TrEMBL
Match: A0A0A0L6R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 6.0e-116
Identity = 208/227 (91.63%), Postives = 218/227 (96.04%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VGS+FFCC+SSNKY RLDAKLGRKM+EVKRN AGHDNFKS+NGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKD+ S TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 238
           ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDDLS T
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLSPT 227

[Close]

BLAST of Tan0002009 vs. ExPASy TrEMBL
Match: A0A6J1EBV7 (probable calcium-binding protein CML22 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431775 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.7e-115
Identity = 205/226 (90.71%), Postives = 217/226 (96.02%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VG+MF CC+SSNKY R+D KLGRKM+EVKRNLAGHDNFKS+NGIILRFPQF+EGLQN
Sbjct: 1   MSKVGNMFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGS+GIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSSGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKDQ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP 
Sbjct: 121 LCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPF 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 237
           ERSPARI +TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL
Sbjct: 181 ERSPARITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL 226

[Close]

BLAST of Tan0002009 vs. ExPASy TrEMBL
Match: A0A6J1ITH1 (probable calcium-binding protein CML22 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479770 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.0e-115
Identity = 205/227 (90.31%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VG+MF CC+SSNKY R+D KLGRKM+EVKRNLAGHDNFKS+NGIILRFPQF+EGLQN
Sbjct: 1   MSKVGNMFLCCSSSNKYNRIDEKLGRKMVEVKRNLAGHDNFKSLNGIILRFPQFREGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVF QYDEDSNGSIDREELKKCLQQLQMHMTE+EV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFAQYDEDSNGSIDREELKKCLQQLQMHMTEDEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKDQ SPTKSKLGSPQLEATFDTIIQAFIFLDKNGDG LNKKEMVKALNEASP 
Sbjct: 121 LCLIYLLKDQHSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGNLNKKEMVKALNEASPF 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 238
           ERSPARI +TRF+EMDWNNSGKVNFREFLFGFINWVGIDTDDDLSL+
Sbjct: 181 ERSPARITKTRFREMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLS 227

[Close]

BLAST of Tan0002009 vs. ExPASy TrEMBL
Match: A0A1S3AVV2 (probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.0e-115
Identity = 206/225 (91.56%), Postives = 217/225 (96.44%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           +S VGS+FFCC+SSNKY RLDAKLGRKM+EVKRN AGHDNFKS+NGIILRFPQFKEGLQN
Sbjct: 1   MSKVGSIFFCCSSSNKYNRLDAKLGRKMVEVKRNSAGHDNFKSLNGIILRFPQFKEGLQN 60

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDREELKKCLQ+LQMHMTEEEV+DLF SCDIDGSAGIQFNEFIVL
Sbjct: 61  IRGVFEQYDEDSNGSIDREELKKCLQKLQMHMTEEEVEDLFHSCDIDGSAGIQFNEFIVL 120

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKD+ S TKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLN+KEMVKALNEASP 
Sbjct: 121 LCLIYLLKDEHSLTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNRKEMVKALNEASPY 180

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLS 236
           ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVG+DTDDDLS
Sbjct: 181 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGVDTDDDLS 225

[Close]

BLAST of Tan0002009 vs. ExPASy TrEMBL
Match: A0A6J1D045 (probable calcium-binding protein CML22 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016137 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.9e-114
Identity = 206/227 (90.75%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 11  VSPVGSMFFCCNSSNKYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQN 70
           VS VGSMF CC+SSNKYKRLDAKLGRKMLEVK+N  GHDNFKS+NGIILRFPQFKEGLQN
Sbjct: 62  VSKVGSMFLCCSSSNKYKRLDAKLGRKMLEVKKNSTGHDNFKSLNGIILRFPQFKEGLQN 121

Query: 71  IRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVL 130
           IRGVFEQYDEDSNGSIDR ELKKCLQQLQMHMTEEEV+DLF+SCDIDGS+GIQFNEFIVL
Sbjct: 122 IRGVFEQYDEDSNGSIDRAELKKCLQQLQMHMTEEEVEDLFRSCDIDGSSGIQFNEFIVL 181

Query: 131 LCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPC 190
           LCLIYLLKD+ S T+SKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNE SP 
Sbjct: 182 LCLIYLLKDEHSQTESKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEGSPS 241

Query: 191 ERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDDLSLT 238
           ERSPARI +TRFKEMDWNNSGKVNFREFLFGFINWVGI  DDDLSLT
Sbjct: 242 ERSPARITKTRFKEMDWNNSGKVNFREFLFGFINWVGIGMDDDLSLT 288

[Close]

BLAST of Tan0002009 vs. TAIR 10
Match: AT3G24110.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.7e-67
Identity = 127/224 (56.70%), Postives = 166/224 (74.11%), Query Frame = 0


Query: 17  MFFCC-NSSN--KYKRLDAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQNIRG 76
           M  CC NS N  KY  LDAKL RKM+E +    GH + KS++ II++FP+ +EGL+NIR 
Sbjct: 1   MLCCCVNSENNKKYAELDAKLARKMVESRSIYPGHRSLKSMDSIIMKFPKLREGLRNIRS 60

Query: 77  VFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCL 136
           VFE YD D+NG+ID EELKKCL++L++ +++EEVK L+  CD+DGS GIQFNEFIVLLCL
Sbjct: 61  VFESYDNDTNGTIDIEELKKCLEELKLSLSDEEVKGLYSWCDVDGSKGIQFNEFIVLLCL 120

Query: 137 IYLL--KDQKSPTKSKLGSPQL-EATFDTIIQAFIFLDKNGDGKLNKKEMVKAL-NEASP 196
           IYLL     +S T+S+   P+L E+ FD I++ F+FLDK+G GKLNK +++K L NE  P
Sbjct: 121 IYLLAKPSSESSTESREMGPKLVESIFDPIVEVFLFLDKDGKGKLNKADVIKTLNNEDYP 180

Query: 197 CERSPARIIQTRFKEMDWNNSGKVNFREFLFGFINWVGIDTDDD 234
            ERSP+ +   RF+EMDW   GKV FREFLF F++WVG+D  DD
Sbjct: 181 LERSPSHVTNMRFEEMDWGRKGKVGFREFLFAFMSWVGLDDADD 224

[Close]

BLAST of Tan0002009 vs. TAIR 10
Match: AT4G26470.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 218.8 bits (556), Expect = 4.7e-57
Identity = 102/206 (49.51%), Postives = 150/206 (72.82%), Query Frame = 0


Query: 31  DAKLGRKMLE-VKRNLAGHDNFKSINGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 90
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 91  ELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCLIYLLKDQKSPTKSK-- 150
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 151 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARIIQTRFKEMDW 210
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF+EMDW
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRFEEMDW 197

Query: 211 NNSGKVNFREFLFGFINWVGIDTDDD 234
           + +G VNF+EFLF F  WVGID +++
Sbjct: 198 DKNGMVNFKEFLFAFTQWVGIDENEE 221

[Close]

BLAST of Tan0002009 vs. TAIR 10
Match: AT4G26470.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.0e-43
Identity = 84/175 (48.00%), Postives = 125/175 (71.43%), Query Frame = 0


Query: 31  DAKLGRKMLE-VKRNLAGHDNFKSINGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDRE 90
           + KL  K++E V+R  +     KS N I+L+FP+  +GL+N + +F+++DEDSNGSID  
Sbjct: 18  ETKLEAKIIEAVQRRASRGTTMKSFNSIVLKFPKIDDGLRNCKAIFQEFDEDSNGSIDHT 77

Query: 91  ELKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCLIYLLKDQKSPTKSK-- 150
           ELK C+++L++   EEE+ DLF++CDI+   GI F EFIVLLCL+YLLKD  S  + K  
Sbjct: 78  ELKNCIRKLEISFDEEEINDLFKACDINEDMGITFTEFIVLLCLVYLLKDDSSTLQKKWT 137

Query: 151 LGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARIIQTRF 203
           +G P+LE TF+T++  F+FLD+N DG ++++EMV+A++E+   ERS  RI   RF
Sbjct: 138 MGMPKLEPTFETLVDTFVFLDENKDGYVSREEMVRAIDESG--ERSSGRIAMKRF 190

[Close]

BLAST of Tan0002009 vs. TAIR 10
Match: AT5G04870.1 (calcium dependent protein kinase 1 )

HSP 1 Score: 70.9 bits (172), Expect = 1.6e-12
Identity = 46/189 (24.34%), Postives = 89/189 (47.09%), Query Frame = 0


Query: 31  DAKLGRKMLEVKRNLAGHDNFKSINGIILRFPQFKEGLQNIRGVFEQYDEDSNGSIDREE 90
           D  L   +L   +  +  + FK +   ++     +E +  ++ +F   D D +G I  EE
Sbjct: 416 DKPLDSAVLSRMKQFSAMNKFKKMALRVIAESLSEEEIAGLKEMFNMIDADKSGQITFEE 475

Query: 91  LKKCLQQLQMHMTEEEVKDLFQSCDIDGSAGIQFNEFIVLLCLIYLLKDQKSPTKSKLGS 150
           LK  L+++  ++ E E+ DL Q+ D+D S  I + EFI     ++L K ++         
Sbjct: 476 LKAGLKRVGANLKESEILDLMQAADVDNSGTIDYKEFIA--ATLHLNKIERE-------- 535

Query: 151 PQLEATFDTIIQAFIFLDKNGDGKLNKKEMVKALNEASPCERSPARIIQTRFKEMDWNNS 210
                  D +  AF + DK+G G +   E+ +A  E    +      I+   +++D +N 
Sbjct: 536 -------DHLFAAFTYFDKDGSGYITPDELQQACEEFGVEDVR----IEELMRDVDQDND 583

Query: 211 GKVNFREFL 220
           G++++ EF+
Sbjct: 596 GRIDYNEFV 583

[Close]

BLAST of Tan0002009 vs. TAIR 10
Match: AT4G04710.1 (calcium-dependent protein kinase 22 )

HSP 1 Score: 69.7 bits (169), Expect = 3.6e-12
Identity = 47/156 (30.13%), Postives = 80/156 (51.28%), Query Frame = 0


Query: 65  KEGLQNIRGVFEQYDEDSNGSIDREELKKCLQQLQMHMTEEEVKDLFQ--SCDIDGSAGI 124
           +E ++ ++ +FE  D D +GSI  EELK  L +    ++E EVK L +  S D+DG+  I
Sbjct: 331 EEEIKGLKTMFENMDMDKSGSITYEELKMGLNRHGSKLSETEVKQLMEAVSADVDGNGTI 390

Query: 125 QFNEFIVLLCLIYLLKDQKSPTKSKLGSPQLEATFDTIIQAFIFLDKNGDGKLNKKEMVK 184
            + EFI                 + +   +LE   + + +AF + DK+G G + K+E+  
Sbjct: 391 DYIEFI----------------SATMHRHRLERD-EHLYKAFQYFDKDGSGHITKEEVEI 450

Query: 185 ALNEASPCERSPARIIQTRFKEMDWNNSGKVNFREF 219
           A+ E    + + A+ +     E D NN GK+++ EF
Sbjct: 451 AMKEHGMGDEANAKDL---ISEFDKNNDGKIDYEEF 466

The following BLAST results are available for this feature:

BLAST of Tan0002009 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRN6	2.4e-66	56.70	Probable calcium-binding protein CML22 OS=Arabidopsis thaliana OX=3702 GN=CML22 ...	[more]
Q52K82	6.7e-56	49.51	Probable calcium-binding protein CML21 OS=Arabidopsis thaliana OX=3702 GN=CML21 ...	[more]
P41210	2.0e-12	30.46	Caltractin OS=Atriplex nummularia OX=3553 PE=2 SV=1	[more]
Q9ZSA3	3.5e-12	29.87	Calcium-dependent protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CPK22 PE=...	[more]
P02588	1.7e-11	27.74	Troponin C, skeletal muscle OS=Gallus gallus OX=9031 GN=TNNC2 PE=1 SV=3	[more]

BLAST of Tan0002009 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892181.1	5.9e-118	92.54	probable calcium-binding protein CML22 isoform X1 [Benincasa hispida]	[more]
XP_011650876.1	1.2e-115	91.63	probable calcium-binding protein CML22 [Cucumis sativus] >XP_031737636.1 probabl...	[more]
XP_038892189.1	2.1e-115	91.59	probable calcium-binding protein CML22 isoform X2 [Benincasa hispida] >XP_038892...	[more]
XP_022924247.1	3.6e-115	90.71	probable calcium-binding protein CML22 isoform X1 [Cucurbita moschata] >XP_02292...	[more]
XP_022980390.1	6.1e-115	90.31	probable calcium-binding protein CML22 isoform X1 [Cucurbita maxima] >XP_0229803...	[more]

BLAST of Tan0002009 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6R7	6.0e-116	91.63	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G130890 PE=4 SV=1	[more]
A0A6J1EBV7	1.7e-115	90.71	probable calcium-binding protein CML22 isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1ITH1	3.0e-115	90.31	probable calcium-binding protein CML22 isoform X1 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A1S3AVV2	3.0e-115	91.56	probable calcium-binding protein CML22 OS=Cucumis melo OX=3656 GN=LOC103483480 P...	[more]
A0A6J1D045	1.9e-114	90.75	probable calcium-binding protein CML22 isoform X1 OS=Momordica charantia OX=3673...	[more]

BLAST of Tan0002009 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24110.1	1.7e-67	56.70	Calcium-binding EF-hand family protein	[more]
AT4G26470.1	4.7e-57	49.51	Calcium-binding EF-hand family protein	[more]
AT4G26470.2	1.0e-43	48.00	Calcium-binding EF-hand family protein	[more]
AT5G04870.1	1.6e-12	24.34	calcium dependent protein kinase 1	[more]
AT4G04710.1	3.6e-12	30.13	calcium-dependent protein kinase 22	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 89..109
None	No IPR available	GENE3D	1.10.238.10		coord: 34..132 e-value: 2.0E-19 score: 71.3
None	No IPR available	GENE3D	1.10.238.10		coord: 133..227 e-value: 4.7E-12 score: 47.6
None	No IPR available	PANTHER	PTHR23064	TROPONIN	coord: 10..235
None	No IPR available	PANTHER	PTHR23064:SF53	CALCIUM-BINDING PROTEIN CML22-RELATED	coord: 10..235
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 160..188 e-value: 0.027 score: 23.6 coord: 107..135 e-value: 1.2 score: 17.0 coord: 198..226 e-value: 5.8 score: 11.6 coord: 71..99 e-value: 0.0075 score: 25.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 159..220 e-value: 1.8E-8 score: 34.7 coord: 71..130 e-value: 6.9E-11 score: 42.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 103..138 score: 9.910664
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 67..102 score: 13.481276
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 156..191 score: 10.663839
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 194..229 score: 8.934325
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 72..132 e-value: 4.87733E-14 score: 62.9505
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 80..92
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 169..181
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 67..233

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0002009.1	Tan0002009.1	mRNA
Tan0002009.2	Tan0002009.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding