Tan0001824 (gene) Snake gourd v1

BLAST of Tan0001824 vs. ExPASy Swiss-Prot
Match: K4CF70 (Fatty acid hydroperoxide lyase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HPL PE=1 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 5.5e-172
Identity = 297/476 (62.39%), Postives = 370/476 (77.73%), Query Frame = 0

Query: 41  MPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTRMEKNRSTVF 100
           M S P S +P PV LPVR+IPG YG PL G IADRLDYFWFQ PE FF  RMEK++STVF
Sbjct: 1   MNSAPLS-TPAPVTLPVRSIPGSYGLPLVGPIADRLDYFWFQKPENFFTKRMEKHKSTVF 60

Query: 101 RTNVPPSFPFF-LTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCA 160
           RTNVPP FPFF   +P V+AVLD KSF+HLFDMEIVEK NVLVGDFMPS  +TG+MRVCA
Sbjct: 61  RTNVPPCFPFFGSVNPNVVAVLDVKSFSHLFDMEIVEKANVLVGDFMPSVVYTGDMRVCA 120

Query: 161 YLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKPASYRIPLQ 220
           YLDTSE +H++IKNF  DIL+R SK W+P L   L T++ T E++++K+   AS    LQ
Sbjct: 121 YLDTSEPKHAQIKNFSQDILKRGSKTWVPTLLKELDTMFTTFEADLSKS-NTASLLPALQ 180

Query: 221 QTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIFLHSFRL 280
           + LF+FFS T+ GADP+ SPE+A SGY+ +  WL +QL PT+ +G+LQPLEEI +HSF  
Sbjct: 181 KFLFNFFSLTILGADPSVSPEIANSGYIFLDSWLAIQLAPTVSIGVLQPLEEILVHSFAY 240

Query: 281 PFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYGGFSLFIPV 340
           PFF + G Y +L  F++ E KEV+ R  T+F L ++EAIHN+LF +GFNA+GGFS+F+P 
Sbjct: 241 PFFLVKGNYEKLVQFVKNEAKEVLSRAQTEFQLTEQEAIHNLLFILGFNAFGGFSIFLPT 300

Query: 341 LLDRLLNDKTG-LQQRIVEEVRAKSG---SGLTFESVKEMKLVYSVVYEALRLDPPVPLQ 400
           LL  L ++K   +Q+++ +EVR K G     L+FESVKEM+LV S VYE LRL PPVP Q
Sbjct: 301 LLGNLGDEKNADMQEKLRKEVRDKVGVNPENLSFESVKEMELVQSFVYETLRLSPPVPSQ 360

Query: 401 YARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAALLD 460
           YARARKDFKLSSHDSV+ IKKGELLCGYQPLVM+DPKVF+EP++F  ERF  EKG  LL+
Sbjct: 361 YARARKDFKLSSHDSVYEIKKGELLCGYQPLVMKDPKVFDEPEKFVLERFTKEKGKELLN 420

Query: 461 YLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQK 512
           YLFWSNGPQTG P+E NKQCA KD+V LTA + VAYIF++YDS++   G++T+++K
Sbjct: 421 YLFWSNGPQTGRPTESNKQCAAKDMVTLTASLIVAYIFQKYDSVSFSSGSLTSVKK 474

BLAST of Tan0001824 vs. ExPASy Swiss-Prot
Match: Q9ZSY9 (Linolenate hydroperoxide lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=1 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-162
Identity = 275/491 (56.01%), Postives = 369/491 (75.15%), Query Frame = 0

Query: 30  MLMKVTMTSGGMPSIPSSIS-----PPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGP 89
           ML++    +   P   +S++      PP  LP+R +PG YGWPL G ++DRLDYFWFQGP
Sbjct: 1   MLLRTMAATSPRPPPSTSLTSQQPPSPPSQLPLRTMPGSYGWPLVGPLSDRLDYFWFQGP 60

Query: 90  EKFFRTRMEKNRSTVFRTNVPPSFPFF-LTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVG 149
           +KFFRTR EK +STVFRTN+PP+FPFF   +P ++AVLD KSF+HLFDM++V+K++VL+G
Sbjct: 61  DKFFRTRAEKYKSTVFRTNIPPTFPFFGNVNPNIVAVLDVKSFSHLFDMDLVDKRDVLIG 120

Query: 150 DFMPSTSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVES 209
           DF PS  F G + V   LDT+E +H+KIK F  + L+RSSK+W+ EL SNL+  W T+ES
Sbjct: 121 DFRPSLGFYGGVCVGVNLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNLNIFWGTIES 180

Query: 210 EIAKNEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHL 269
           EI+KN   ASY  PLQ+ +FSF   +LAG D + SP++A++G+  I  WL LQ++PT  L
Sbjct: 181 EISKN-GAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLALQVIPTAKL 240

Query: 270 GIL-QPLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNIL 329
           G++ QPLEEI LH++  P   IAG Y++L +FI++   + ++ G  +F L ++EAI N+L
Sbjct: 241 GVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFRLTRDEAIQNLL 300

Query: 330 FTMGFNAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSG--LTFESVKEMKLVYS 389
           F +GFNAYGGFS+F+P L+ R+  D +GLQ+RI  EVR   GSG  L F++V EM+LV S
Sbjct: 301 FVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNFKTVNEMELVKS 360

Query: 390 VVYEALRLDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEF 449
           VVYE LR +PPVPLQ+ARARKDF++SSHD+VF +KKGELLCGYQPLVMRD  VF+EP+EF
Sbjct: 361 VVYETLRFNPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDANVFDEPEEF 420

Query: 450 RPERFMGEKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIA 509
           +P+R++GE G+ LL+YL+WSNGPQTG PS  NKQCA KD+V LTA + VA +F RYD+I 
Sbjct: 421 KPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVADLFLRYDTIT 480

Query: 510 GEGGTITALQK 512
           G+ G+I A+ K
Sbjct: 481 GDSGSIKAVVK 490

BLAST of Tan0001824 vs. ExPASy Swiss-Prot
Match: B3LF83 (Probable inactive linolenate hydroperoxide lyase OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.2e-127
Identity = 217/383 (56.66%), Postives = 292/383 (76.24%), Query Frame = 0

Query: 132 MEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELE 191
           M++V+K++VL+GDF PS  F G +RV  YLDT+E +H+KIK F  + L+RSSK+W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 192 SNLSTLWDTVESEIAKNEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIII 251
           SNL+  W T+ESEI+KN   ASY  PLQ+ +FSF   +LAG D + SP++A++G+  I  
Sbjct: 61  SNLNIFWGTIESEISKN-GAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINT 120

Query: 252 WLGLQLVPTIHLGIL-QPLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDF 311
           WL LQ++PT  LG++ QPLEEI LH++  P   IAG Y++L +FI++   + ++ G  +F
Sbjct: 121 WLALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEF 180

Query: 312 GLKKEEAIHNILFTMGFNAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSG--LT 371
           GL ++EAI N+LF +GFNAYGGFS+F+P L+ R+  D +GLQ+RI  EVR   GSG  L 
Sbjct: 181 GLTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLN 240

Query: 372 FESVKEMKLVYSVVYEALRLDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVM 431
           F++V EM+LV SVVYE LR  PPVPLQ+ARARKDF++SSHD+VF +KKGELLCGYQPLVM
Sbjct: 241 FKTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVM 300

Query: 432 RDPKVFEEPDEFRPERFMGEKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVF 491
           RD  VF+EP+EF+P+R++GE G+ LL+YL+WSNGPQTG PS  NKQCA KD+V LTA + 
Sbjct: 301 RDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLL 360

Query: 492 VAYIFRRYDSIAGEGGTITALQK 512
           VA +F RYD+I G+ G+I A+ K
Sbjct: 361 VADLFLRYDTITGDSGSIKAVVK 382

BLAST of Tan0001824 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 1.9e-108
Identity = 205/483 (42.44%), Postives = 295/483 (61.08%), Query Frame = 0

Query: 32  MKVTMTSGGMPSIPSSISPPP-----VALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEK 91
           +K ++       IP   S PP       LP+R IPG YG P  G I DRLDYF+ QG E+
Sbjct: 42  IKASLFGDSPIKIPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREE 101

Query: 92  FFRTRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFM 151
           FF++R++K +STV+R N+PP  PF  ++PRVI +LD KSF  LFDM  VEKK++  G +M
Sbjct: 102 FFKSRLQKYKSTVYRANMPPG-PFIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYM 161

Query: 152 PSTSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIA 211
           PST  TG  R+ +YLD SE  H+K+K  L ++++      IPE  S+ + L + VE ++A
Sbjct: 162 PSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLA 221

Query: 212 KNEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGIL 271
              K A++  P +Q  F+F S+   G  P  +P + K     I  W+   L P + +G+ 
Sbjct: 222 TKGK-AAFNDPAEQAAFNFLSRAFFGVKPIDTP-LGKDAPSLISKWVLFNLAPILSVGLP 281

Query: 272 QPLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMG 331
           + +EE  LHS RLP   +   Y RL +F       V+       G+ ++EA HNILF + 
Sbjct: 282 KEVEEATLHSVRLPPLLVQNDYHRLYEFFTSAAGSVLDE-AEQSGISRDEACHNILFAVC 341

Query: 332 FNAYGGFSLFIPVLLDRL----LNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVV 391
           FN++GGF +  P L+  +    L   T L Q I   +++  G  +T  ++++M L+ SVV
Sbjct: 342 FNSWGGFKILFPSLMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVV 401

Query: 392 YEALRLDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRP 451
           YE LR++PPV LQY +A+KDF L SH++ + +K+GE+L GYQP   +DPK+F+ P+EF  
Sbjct: 402 YETLRIEPPVALQYGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVA 461

Query: 452 ERFMGEKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGE 506
           +RF+GE G  L++Y+ WSNGP+T  PS  NKQCAGKD VV+ A +FV  +F+RYDS   E
Sbjct: 462 DRFVGE-GVKLMEYVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIE 519

BLAST of Tan0001824 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 389.4 bits (999), Expect = 6.2e-107
Identity = 202/455 (44.40%), Postives = 288/455 (63.30%), Query Frame = 0

Query: 55  LPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTRMEKNRSTVFRTNVPPSFPFFLTD 114
           LP+RNIPG YG P+ G I DR DYF+ QG E+FF++R+ K  STV+R N+PP   F   +
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPG-AFIAEN 111

Query: 115 PRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDTSESQHSKIKNF 174
           P+V+A+LD KSF  LFD++ VEKK++  G +MPST  TG  R+ +YLD SE +H K+KN 
Sbjct: 112 PQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNL 171

Query: 175 LTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKPASYRIPLQQTLFSFFSKTLAGAD 234
           L  +L+ S     PE ++  S L+D++E E++   K A +      T F+F ++   G +
Sbjct: 172 LFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAFYGTN 231

Query: 235 PAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIFLHSFRLPFFPIAGRYRRLSD- 294
           PA +   A +  L I  W+   L P + +G+ + +EE  +H+F LP   +   Y+RL + 
Sbjct: 232 PADTKLKADAPGL-ITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 295 FIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYGGFSLFIPVLLDRLLNDKTGLQQ 354
           F+E  G+ +V+      G+ +EEA HN+LF   FN +GG  +  P ++ R+      +  
Sbjct: 292 FLESAGEILVE--ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHN 351

Query: 355 RIVEEVRA---KSGSGLTFESVKEMKLVYSVVYEALRLDPPVPLQYARARKDFKLSSHDS 414
           R+ EE+R+    +G  LT  ++++M+L  SVVYE LR +PPV  QY RA+KD  + SHD+
Sbjct: 352 RLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDA 411

Query: 415 VFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAALLDYLFWSNGPQTGMPSE 474
            F +K GE+L GYQPL  RDPK+F+  DEF PERF+GE+G  LL ++ WSNGP+T  P+ 
Sbjct: 412 AFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTV 471

Query: 475 QNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGT 506
            NKQCAGKD VVL A +FV  IFRRYDS   E GT
Sbjct: 472 GNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGT 501

BLAST of Tan0001824 vs. NCBI nr
Match: XP_022952346.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 859.8 bits (2220), Expect = 1.2e-245
Identity = 416/485 (85.77%), Postives = 454/485 (93.61%), Query Frame = 0

Query: 28  NLMLMKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKF 87
           NLM   V M SGG+PSIPSSISPPPV LP++NIPG +G PLFGSIADRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 88  FRTRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 147
           FR+R+EKNRSTVFRTNVPPSFPFFL+DP+VIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 148 STSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAK 207
           STSFTGNMR+CAYLD SESQHSKIKNF  DILRRSSKIWIPELESNLST WD +ESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 208 NEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQ 267
           N+K ++YR  LQ  LF+FFSKTLAGAD AKSPEVAKS Y+D+IIWLGLQLVPTIHLGILQ
Sbjct: 230 NQK-STYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGILQ 289

Query: 268 PLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGF 327
           PLEEIFLHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKKEEAIHN++FT+GF
Sbjct: 290 PLEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGF 349

Query: 328 NAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALR 387
           NAYGGFSLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS+VYEALR
Sbjct: 350 NAYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYEALR 409

Query: 388 LDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMG 447
           LDPPVP QYARARK+FKLSSHD+VF IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  
Sbjct: 410 LDPPVPTQYARARKEFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFRE 469

Query: 448 EKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTIT 507
           EKGAALLDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+IT
Sbjct: 470 EKGAALLDYLFWSNGPQTGMSSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSIT 529

Query: 508 ALQKP 513
           ALQKP
Sbjct: 530 ALQKP 533

BLAST of Tan0001824 vs. NCBI nr
Match: KAG6572283.1 (Fatty acid hydroperoxide lyase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.6e-245
Identity = 415/485 (85.57%), Postives = 452/485 (93.20%), Query Frame = 0

Query: 28  NLMLMKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKF 87
           NLM   V M SGG+PSIPSSISPPPV LP++NIPG +G PLFGSIADRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 88  FRTRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 147
           FR+R+EKNRSTVFRTNVPPSFPFFL+DP+VIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 148 STSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAK 207
           STSFTGNMR+CAYLD SESQHSKIKN+  DILRRSSKIWIPELESNLST WD +ESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNYTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 208 NEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQ 267
           N+K +SYR  LQ  LF+FFSKTLAGAD AKSPEVAKS Y+D+IIWLGLQLVPTIHLGI Q
Sbjct: 230 NQK-SSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGIFQ 289

Query: 268 PLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGF 327
           PLEEIFLHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKKEEAIHN++FT+GF
Sbjct: 290 PLEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGF 349

Query: 328 NAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALR 387
           NAYGGFSLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS+VYE LR
Sbjct: 350 NAYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLR 409

Query: 388 LDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMG 447
           LDPPVP QYARARKDFKLSSHD+VF IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  
Sbjct: 410 LDPPVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFRE 469

Query: 448 EKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTIT 507
           EKGAALLDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+IT
Sbjct: 470 EKGAALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSIT 529

Query: 508 ALQKP 513
           ALQKP
Sbjct: 530 ALQKP 533

BLAST of Tan0001824 vs. NCBI nr
Match: KAG7011899.1 (Fatty acid hydroperoxide lyase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854.4 bits (2206), Expect = 5.2e-244
Identity = 413/483 (85.51%), Postives = 450/483 (93.17%), Query Frame = 0

Query: 30  MLMKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFR 89
           M   V M SGG+PSIPSSISPPPV LP++NIPG +G PLFGSIADRLDYFWFQGP+KFFR
Sbjct: 1   MSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFR 60

Query: 90  TRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPST 149
           +R+EKNRSTVFRTNVPPSFPFFL+DP+VIAVLDCKSFAHLFDME+VEKKNVLVGDFMPST
Sbjct: 61  SRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPST 120

Query: 150 SFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNE 209
           SFTGNMR+CAYLD SESQHSKIKN+  DILRRSSKIWIPELESNLST WD +ESEIA N+
Sbjct: 121 SFTGNMRMCAYLDPSESQHSKIKNYTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQ 180

Query: 210 KPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPL 269
           K +SYR  LQ  LF+FFSKTLAGAD AKSPEVAKS Y+D+IIWLGLQLVPTIHLGI QPL
Sbjct: 181 K-SSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGIFQPL 240

Query: 270 EEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNA 329
           EEIFLHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKKEEAIHN++FT+GFNA
Sbjct: 241 EEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFNA 300

Query: 330 YGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLD 389
           YGGFSLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS+VYE LRLD
Sbjct: 301 YGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRLD 360

Query: 390 PPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEK 449
           PPVP QYARARKDFKLSSHD+VF IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  EK
Sbjct: 361 PPVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEK 420

Query: 450 GAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITAL 509
           GAALLDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+ITAL
Sbjct: 421 GAALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITAL 480

Query: 510 QKP 513
           QKP
Sbjct: 481 QKP 482

BLAST of Tan0001824 vs. NCBI nr
Match: AAU12570.1 (13-hydroperoxide lyase [Citrullus lanatus])

HSP 1 Score: 854.0 bits (2205), Expect = 6.8e-244
Identity = 412/480 (85.83%), Postives = 450/480 (93.75%), Query Frame = 0

Query: 32  MKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTR 91
           MKVTMTSGGMPSIPSSISPPPV LP+RNIPG YG PLFGSI DRLDYFWFQGPEKFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 92  MEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 151
           MEKN+STVFRTNVPPSFPFF TDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 152 TGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKP 211
           TGNMRVCAYLDTSESQHSKIKNF+ D+LRRSS+IWI ELESNLST+WD++ESEIAK+ K 
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTK- 180

Query: 212 ASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEE 271
           +S+R  LQ TLF+FFSKTLAGAD AKSPEVAKSGY+D+IIWLGLQLVPTIH+GILQPLEE
Sbjct: 181 SSFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEE 240

Query: 272 IFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYG 331
           IFLHSFRLPFFPIA RY+RL DFI+KEG+EVV+RGV++FGL K+EAIHN++FTMGFNAYG
Sbjct: 241 IFLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYG 300

Query: 332 GFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDPP 391
           GFSLF PVLLDR+LNDKTGLQQRI+EEV+AK+GSGLTFESVKEM L+YSVVYE LRLDPP
Sbjct: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPP 360

Query: 392 VPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGA 451
           VP QYARARKDFKLSS+DS ++IKKGELLCGYQPLVMRDPKVF +P  F P RF GEKGA
Sbjct: 361 VPTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGA 420

Query: 452 ALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQK 511
           ALLDYLFWSNGPQTG+PSE NKQCAGKD+VVLTAVVFVAYIFRRYD IAGEGG+ITA Q+
Sbjct: 421 ALLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQR 479

BLAST of Tan0001824 vs. NCBI nr
Match: XP_023554457.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 853.6 bits (2204), Expect = 8.9e-244
Identity = 413/483 (85.51%), Postives = 449/483 (92.96%), Query Frame = 0

Query: 30  MLMKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFR 89
           M   V M SGG+PSIPSSISPPPVALP++NIPG +G PLFGSIADRLDYFWFQGP+KFFR
Sbjct: 1   MSTMVAMASGGVPSIPSSISPPPVALPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFR 60

Query: 90  TRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPST 149
           +R+EKNRSTVFRTNVPPSFPF L+DP+VIAVLDCKSFAHLFDME+VEKKNVLVGDFMPST
Sbjct: 61  SRIEKNRSTVFRTNVPPSFPFILSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPST 120

Query: 150 SFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNE 209
           SFTGNMRVCAYLD SESQHSKIKNF  DILRRSSKIWIPELESNLST WD +ESEIA N+
Sbjct: 121 SFTGNMRVCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQ 180

Query: 210 KPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPL 269
           K +SYR  LQ  LF+FFSKTLAGAD AKSPEVAKS Y+D+IIWLGLQLVPTIHLG+ QPL
Sbjct: 181 K-SSYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGVFQPL 240

Query: 270 EEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNA 329
           EEIFLHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKKEEAIHN++F +GFNA
Sbjct: 241 EEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFALGFNA 300

Query: 330 YGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLD 389
           YGGFSLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS+VYE LRLD
Sbjct: 301 YGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRLD 360

Query: 390 PPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEK 449
           PPVP QYARARKDFKLSSHD+VF IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  EK
Sbjct: 361 PPVPTQYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEK 420

Query: 450 GAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITAL 509
           GAALLDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+ITAL
Sbjct: 421 GAALLDYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITAL 480

Query: 510 QKP 513
           QKP
Sbjct: 481 QKP 482

BLAST of Tan0001824 vs. ExPASy TrEMBL
Match: A0A6J1GJZ6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455056 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.0e-246
Identity = 416/485 (85.77%), Postives = 454/485 (93.61%), Query Frame = 0

Query: 28  NLMLMKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKF 87
           NLM   V M SGG+PSIPSSISPPPV LP++NIPG +G PLFGSIADRLDYFWFQGP+KF
Sbjct: 50  NLMSTMVAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKF 109

Query: 88  FRTRMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMP 147
           FR+R+EKNRSTVFRTNVPPSFPFFL+DP+VIAVLDCKSFAHLFDME+VEKKNVLVGDFMP
Sbjct: 110 FRSRIEKNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMP 169

Query: 148 STSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAK 207
           STSFTGNMR+CAYLD SESQHSKIKNF  DILRRSSKIWIPELESNLST WD +ESEIA 
Sbjct: 170 STSFTGNMRMCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIAN 229

Query: 208 NEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQ 267
           N+K ++YR  LQ  LF+FFSKTLAGAD AKSPEVAKS Y+D+IIWLGLQLVPTIHLGILQ
Sbjct: 230 NQK-STYRNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGILQ 289

Query: 268 PLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGF 327
           PLEEIFLHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKKEEAIHN++FT+GF
Sbjct: 290 PLEEIFLHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGF 349

Query: 328 NAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALR 387
           NAYGGFSLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS+VYEALR
Sbjct: 350 NAYGGFSLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYEALR 409

Query: 388 LDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMG 447
           LDPPVP QYARARK+FKLSSHD+VF IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  
Sbjct: 410 LDPPVPTQYARARKEFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFRE 469

Query: 448 EKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTIT 507
           EKGAALLDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+IT
Sbjct: 470 EKGAALLDYLFWSNGPQTGMSSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSIT 529

Query: 508 ALQKP 513
           ALQKP
Sbjct: 530 ALQKP 533

BLAST of Tan0001824 vs. ExPASy TrEMBL
Match: Q66UT1 (13-hydroperoxide lyase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 3.3e-244
Identity = 412/480 (85.83%), Postives = 450/480 (93.75%), Query Frame = 0

Query: 32  MKVTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTR 91
           MKVTMTSGGMPSIPSSISPPPV LP+RNIPG YG PLFGSI DRLDYFWFQGPEKFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 92  MEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 151
           MEKN+STVFRTNVPPSFPFF TDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 152 TGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKP 211
           TGNMRVCAYLDTSESQHSKIKNF+ D+LRRSS+IWI ELESNLST+WD++ESEIAK+ K 
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTK- 180

Query: 212 ASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEE 271
           +S+R  LQ TLF+FFSKTLAGAD AKSPEVAKSGY+D+IIWLGLQLVPTIH+GILQPLEE
Sbjct: 181 SSFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEE 240

Query: 272 IFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYG 331
           IFLHSFRLPFFPIA RY+RL DFI+KEG+EVV+RGV++FGL K+EAIHN++FTMGFNAYG
Sbjct: 241 IFLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYG 300

Query: 332 GFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDPP 391
           GFSLF PVLLDR+LNDKTGLQQRI+EEV+AK+GSGLTFESVKEM L+YSVVYE LRLDPP
Sbjct: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPP 360

Query: 392 VPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGA 451
           VP QYARARKDFKLSS+DS ++IKKGELLCGYQPLVMRDPKVF +P  F P RF GEKGA
Sbjct: 361 VPTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGA 420

Query: 452 ALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQK 511
           ALLDYLFWSNGPQTG+PSE NKQCAGKD+VVLTAVVFVAYIFRRYD IAGEGG+ITA Q+
Sbjct: 421 ALLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQR 479

BLAST of Tan0001824 vs. ExPASy TrEMBL
Match: A0A1S4E196 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495615 PE=4 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.0e-242
Identity = 409/481 (85.03%), Postives = 449/481 (93.35%), Query Frame = 0

Query: 32  MKVTMT-SGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRT 91
           M +TMT SGGMPSIPSSISPPPV+LP+RNIPG YG+PL GSI+DRLDY+WFQGP+KFFRT
Sbjct: 1   MHLTMTSSGGMPSIPSSISPPPVSLPLRNIPGSYGFPLLGSISDRLDYYWFQGPDKFFRT 60

Query: 92  RMEKNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTS 151
           RMEKNRSTVFRTNVPPSFPFF TDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPS S
Sbjct: 61  RMEKNRSTVFRTNVPPSFPFFPTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSIS 120

Query: 152 FTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEK 211
           FTGNMRVCAYLDTSESQHSKIKNF+TDILRRSS+IWI ELESNLST+WD +ESEIAKN K
Sbjct: 121 FTGNMRVCAYLDTSESQHSKIKNFITDILRRSSRIWISELESNLSTMWDGIESEIAKN-K 180

Query: 212 PASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLE 271
            + +R  LQ  LF+FFSKTLAGAD AKSPEVAKSGY+D+IIWLGLQLVPTIH+G+LQPLE
Sbjct: 181 TSGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLE 240

Query: 272 EIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAY 331
           EIFLHSFRLPFFPIA RY+RL DF +KEGKEVV+RGVT+FGL KEEAIHN++FTMGFNAY
Sbjct: 241 EIFLHSFRLPFFPIASRYQRLYDFFQKEGKEVVERGVTEFGLTKEEAIHNLIFTMGFNAY 300

Query: 332 GGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDP 391
           GGFSLF PVLLDR+LNDKTGLQQRI+EEVR+KS SGLTFESVKEM L+YS+VYE LRLDP
Sbjct: 301 GGFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSLVYETLRLDP 360

Query: 392 PVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKG 451
           PVP QYARARKDFKL+S+DS +NIKKGELLCGYQPLVMRDPKVF+EP+ F P+RF GEKG
Sbjct: 361 PVPSQYARARKDFKLTSYDSAYNIKKGELLCGYQPLVMRDPKVFDEPEAFNPDRFRGEKG 420

Query: 452 AALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQ 511
            ALLDYLFWSNGPQTG PSE+NKQCAGKD+VVLTAVVFVAYIF+RYDSIAGEGG+ITA Q
Sbjct: 421 VALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTAVVFVAYIFKRYDSIAGEGGSITAFQ 480

BLAST of Tan0001824 vs. ExPASy TrEMBL
Match: A0A6J1HZX6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111468182 PE=4 SV=1)

HSP 1 Score: 844.7 bits (2181), Expect = 2.0e-241
Identity = 407/479 (84.97%), Postives = 445/479 (92.90%), Query Frame = 0

Query: 34  VTMTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTRME 93
           V M S G+PSIPSSISPPPV LP++NIPG +G PLFGSIADRLDYFWFQGP+KFFR+RME
Sbjct: 2   VAMASSGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRME 61

Query: 94  KNRSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTG 153
           KNRSTVFRTNVPPSFPF L+DP+V AVLDCKSFAHLFDME+VEKKNVLVGDFMPSTSFTG
Sbjct: 62  KNRSTVFRTNVPPSFPFILSDPKVTAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 121

Query: 154 NMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKPAS 213
           NMRVCAYLDTSESQHSKIKNF  DILRRSSKIWIPELESNLST WD +ESEIA N+K +S
Sbjct: 122 NMRVCAYLDTSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQK-SS 181

Query: 214 YRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIF 273
           +R  LQ  LF+FFSKTL GAD AKSPEVAKS ++D+IIWLGLQLVPTIHLGI QPLEEIF
Sbjct: 182 FRNLLQPALFNFFSKTLVGADTAKSPEVAKSAHIDVIIWLGLQLVPTIHLGIFQPLEEIF 241

Query: 274 LHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYGGF 333
           LHSFRLPFFPIAGRYRRL DFIEKEG+EVVQRGV++FGLKK+EAIHN++FT+GFNAYGGF
Sbjct: 242 LHSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKDEAIHNLIFTLGFNAYGGF 301

Query: 334 SLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDPPVP 393
           SLF+PVL+DR+LND+TGLQQRIV+EVRAKSGSGLTFESVKEM+LVYS VYE LRLDPPVP
Sbjct: 302 SLFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSFVYETLRLDPPVP 361

Query: 394 LQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAAL 453
            QYARARKDFKLSSHD+VF+IKKGELLCGYQPLVMRDP VFEEP+ F+P+RF  EKGAAL
Sbjct: 362 TQYARARKDFKLSSHDAVFDIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAAL 421

Query: 454 LDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQKP 513
           LDYLFWSNGPQTGM SE NKQCA KD+VVLTAVVFVAYIFRRYDSIAGEGG+IT LQ+P
Sbjct: 422 LDYLFWSNGPQTGMASEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITTLQRP 479

BLAST of Tan0001824 vs. ExPASy TrEMBL
Match: A0A0A0K5I0 (13-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G041960 PE=4 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 7.3e-236
Identity = 394/476 (82.77%), Postives = 437/476 (91.81%), Query Frame = 0

Query: 36  MTSGGMPSIPSSISPPPVALPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTRMEKN 95
           MTSGGMPSIPSSISPPPV+LP+RNIPG YG PL GSI DRLDY+WFQGP+KFFRTRMEKN
Sbjct: 1   MTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIGDRLDYYWFQGPDKFFRTRMEKN 60

Query: 96  RSTVFRTNVPPSFPFFLTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNM 155
           RSTVFRTNVPPSFPF   DPRV+AVLDCKSFAHLFDMEIVEK NVLVGDFMPS SFTGNM
Sbjct: 61  RSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNM 120

Query: 156 RVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKPASYR 215
           RVCAYLDTSE  HSK+KNF+TDILRRSS+IWI ELESNLST+WD +E E+AKN K + +R
Sbjct: 121 RVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKN-KQSGFR 180

Query: 216 IPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIFLH 275
             LQ  LF+FFSKTLAGAD AKSPEVAKSGY+D+IIWLGLQLVPTIH+G+LQPLEEIFLH
Sbjct: 181 NFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLH 240

Query: 276 SFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYGGFSL 335
           SFRLPFFPIA RY+RL DF +KEG EVV+RGVT+FGL KEEAIHN++FTMGFNAYGGFSL
Sbjct: 241 SFRLPFFPIASRYQRLYDFFQKEGAEVVERGVTEFGLTKEEAIHNLIFTMGFNAYGGFSL 300

Query: 336 FIPVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDPPVPLQ 395
           F PVLLDR+LNDKTGLQQRI++EVR+KS SGLTFESVKEM L+YS+VYE LRLDPPVP Q
Sbjct: 301 FFPVLLDRILNDKTGLQQRILKEVRSKSSSGLTFESVKEMDLIYSIVYETLRLDPPVPSQ 360

Query: 396 YARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAALLD 455
           YARARKDFKL+S++S +NIKKGELLCGYQPLVMRDP+VF+EP+ F P+RF GEKGAALLD
Sbjct: 361 YARARKDFKLTSYNSTYNIKKGELLCGYQPLVMRDPEVFDEPEAFNPDRFRGEKGAALLD 420

Query: 456 YLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGTITALQK 512
           YLFWSNGPQTG PSE+NKQCAGKD+VVLT VVFVAYIF+RYDSIAGEGG+ITA Q+
Sbjct: 421 YLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQR 475

BLAST of Tan0001824 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 456.8 bits (1174), Expect = 2.3e-128
Identity = 217/383 (56.66%), Postives = 292/383 (76.24%), Query Frame = 0

Query: 132 MEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDTSESQHSKIKNFLTDILRRSSKIWIPELE 191
           M++V+K++VL+GDF PS  F G +RV  YLDT+E +H+KIK F  + L+RSSK+W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 192 SNLSTLWDTVESEIAKNEKPASYRIPLQQTLFSFFSKTLAGADPAKSPEVAKSGYLDIII 251
           SNL+  W T+ESEI+KN   ASY  PLQ+ +FSF   +LAG D + SP++A++G+  I  
Sbjct: 61  SNLNIFWGTIESEISKN-GAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINT 120

Query: 252 WLGLQLVPTIHLGIL-QPLEEIFLHSFRLPFFPIAGRYRRLSDFIEKEGKEVVQRGVTDF 311
           WL LQ++PT  LG++ QPLEEI LH++  P   IAG Y++L +FI++   + ++ G  +F
Sbjct: 121 WLALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEF 180

Query: 312 GLKKEEAIHNILFTMGFNAYGGFSLFIPVLLDRLLNDKTGLQQRIVEEVRAKSGSG--LT 371
           GL ++EAI N+LF +GFNAYGGFS+F+P L+ R+  D +GLQ+RI  EVR   GSG  L 
Sbjct: 181 GLTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLN 240

Query: 372 FESVKEMKLVYSVVYEALRLDPPVPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVM 431
           F++V EM+LV SVVYE LR  PPVPLQ+ARARKDF++SSHD+VF +KKGELLCGYQPLVM
Sbjct: 241 FKTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVM 300

Query: 432 RDPKVFEEPDEFRPERFMGEKGAALLDYLFWSNGPQTGMPSEQNKQCAGKDVVVLTAVVF 491
           RD  VF+EP+EF+P+R++GE G+ LL+YL+WSNGPQTG PS  NKQCA KD+V LTA + 
Sbjct: 301 RDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLL 360

Query: 492 VAYIFRRYDSIAGEGGTITALQK 512
           VA +F RYD+I G+ G+I A+ K
Sbjct: 361 VADLFLRYDTITGDSGSIKAVVK 382

BLAST of Tan0001824 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 389.4 bits (999), Expect = 4.4e-108
Identity = 202/455 (44.40%), Postives = 288/455 (63.30%), Query Frame = 0

Query: 55  LPVRNIPGGYGWPLFGSIADRLDYFWFQGPEKFFRTRMEKNRSTVFRTNVPPSFPFFLTD 114
           LP+RNIPG YG P+ G I DR DYF+ QG E+FF++R+ K  STV+R N+PP   F   +
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPG-AFIAEN 111

Query: 115 PRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSFTGNMRVCAYLDTSESQHSKIKNF 174
           P+V+A+LD KSF  LFD++ VEKK++  G +MPST  TG  R+ +YLD SE +H K+KN 
Sbjct: 112 PQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNL 171

Query: 175 LTDILRRSSKIWIPELESNLSTLWDTVESEIAKNEKPASYRIPLQQTLFSFFSKTLAGAD 234
           L  +L+ S     PE ++  S L+D++E E++   K A +      T F+F ++   G +
Sbjct: 172 LFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAFYGTN 231

Query: 235 PAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIFLHSFRLPFFPIAGRYRRLSD- 294
           PA +   A +  L I  W+   L P + +G+ + +EE  +H+F LP   +   Y+RL + 
Sbjct: 232 PADTKLKADAPGL-ITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 295 FIEKEGKEVVQRGVTDFGLKKEEAIHNILFTMGFNAYGGFSLFIPVLLDRLLNDKTGLQQ 354
           F+E  G+ +V+      G+ +EEA HN+LF   FN +GG  +  P ++ R+      +  
Sbjct: 292 FLESAGEILVE--ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHN 351

Query: 355 RIVEEVRA---KSGSGLTFESVKEMKLVYSVVYEALRLDPPVPLQYARARKDFKLSSHDS 414
           R+ EE+R+    +G  LT  ++++M+L  SVVYE LR +PPV  QY RA+KD  + SHD+
Sbjct: 352 RLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDA 411

Query: 415 VFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAALLDYLFWSNGPQTGMPSE 474
            F +K GE+L GYQPL  RDPK+F+  DEF PERF+GE+G  LL ++ WSNGP+T  P+ 
Sbjct: 412 AFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTV 471

Query: 475 QNKQCAGKDVVVLTAVVFVAYIFRRYDSIAGEGGT 506
            NKQCAGKD VVL A +FV  IFRRYDS   E GT
Sbjct: 472 GNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGT 501

BLAST of Tan0001824 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 64.3 bits (155), Expect = 3.3e-10
Identity = 36/142 (25.35%), Postives = 65/142 (45.77%), Query Frame = 0

Query: 361 AKSGSGLTFESVKEMKLVYSVVYEALRLDPPVPLQYARARKDFKLSSHD-SVFNIKKGEL 420
           AK G  +  + + EM ++Y  + EALRL PP+ +    +  DF +++ D   ++I KG +
Sbjct: 323 AKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHI 382

Query: 421 LCGYQPLVMRDPKVFEEPDEFRPERFM----GEKGAALLDYLFWSNGPQTGMPSEQNKQC 480
           +        R P +F++PD + PERF      +K A    Y+ +  G            C
Sbjct: 383 VATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGG---------RHGC 442

Query: 481 AGKDVVVLTAVVFVAYIFRRYD 498
            G+    L      +++ R ++
Sbjct: 443 LGEPFAYLQIKAIWSHLLRNFE 455

BLAST of Tan0001824 vs. TAIR 10
Match: AT3G20110.1 (cytochrome P450, family 705, subfamily A, polypeptide 20 )

HSP 1 Score: 61.2 bits (147), Expect = 2.8e-09
Identity = 76/298 (25.50%), Postives = 121/298 (40.60%), Query Frame = 0

Query: 179 LRRSSKIWIPELESNLSTLWDTV----ESEIAKNEKPASYRIPLQQTLFSFFSKTLAGAD 238
           L RS  I   ELE    +L D        EI K     S     + ++   FS+    A+
Sbjct: 149 LERSRSIRADELERFYRSLLDKAMKKESVEIGKEATKLSINSICRMSMGRSFSEESGEAE 208

Query: 239 PAKSPEVAKSGYLDIIIWLGLQLVPTIHLGILQPLEEIFLHSFRLPFFPIAGRYRRLSD- 298
             +       G    ++ + +         +  PLE++ +  F+     ++  +  L + 
Sbjct: 209 RVRGLVTELDGLTKKVLLVNI---------LRWPLEKLRISLFKKEIMYVSNSFDELLER 268

Query: 299 -FIEKEGKEVVQRG-----VTDFGLKKEEAIHNILFTMGFNAYGGFSLFIPVLLD----- 358
             +E+E K    +G     V     + E+A H I            SLF+ +LL      
Sbjct: 269 IIVEREKKPNEHQGTYLMDVLLEAYEDEKAEHKI------TRNHIKSLFVELLLGGTDTS 328

Query: 359 ---------RLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVY--SVVYEALRLDPP 418
                     L+N++  L +R+ EE+ +  G     +     KL Y  SVV E LRL PP
Sbjct: 329 AQTIQWTMAELINNRNVL-KRLREEIDSVVGETRLIQEKDLPKLPYLQSVVKEGLRLHPP 388

Query: 419 VPLQYARARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEK 450
           +PL      + F+ S     F I +   L      VMRDP  +E+PDEF+PERF+ ++
Sbjct: 389 LPLMV----RTFQRSCEMKGFYIAEKTTLVVNAYAVMRDPTTWEDPDEFKPERFLRQE 426

BLAST of Tan0001824 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 57.8 bits (138), Expect = 3.0e-08
Identity = 42/159 (26.42%), Postives = 71/159 (44.65%), Query Frame = 0

Query: 338 PVLLDRLLNDKTGLQQRIVEEVRAKSGSGLTFESVKEMKLVYSVVYEALRLDPPVPLQYA 397
           P +  R+L +    Q+ I++E + K   GL +E +++M+  ++V  E +R+ PP+   + 
Sbjct: 307 PHVYQRVLQE----QKEILKEKKEK--EGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFR 366

Query: 398 RARKDFKLSSHDSVFNIKKGELLCGYQPLVMRDPKVFEEPDEFRPERFMGEKGAALLDYL 457
            A   F        F I KG  L         +P  F EP+ F P RF G  G     Y+
Sbjct: 367 EAIDHFSFKG----FYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEG-SGPKPYTYV 426

Query: 458 FWSNGPQTGMPSEQNKQCAGKDVVVLTAVVFVAYIFRRY 497
            +  GP         + C GK+   L  ++F+  +  R+
Sbjct: 427 PFGGGP---------RMCPGKEYARLEILIFMHNLVNRF 445