Tan0001461 (gene) Snake gourd v1

BLAST of Tan0001461 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 3.7e-240
Identity = 418/530 (78.87%), Postives = 461/530 (86.98%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRR--NSFLPFTIACSSATSDPAASPVLNSSPPLGVQ 60
           MA+S    +S +SP    L  +   +  N    FT   ++AT+D  +            +
Sbjct: 1   MAASCMLRSSFISPGLPQLHHQSTSKPNNGIHFFTPIKATATNDAISQQ----------K 60

Query: 61  NHQLAGANIQDESIL-CRSVRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQH 120
           + + A  NI++E+   C S  +NFSA+YVPF +G +S EWYSLDEIVYRS+SGGLLDVQH
Sbjct: 61  HRRPADENIREEARRHCSS--HNFSARYVPFNAGPNSDEWYSLDEIVYRSRSGGLLDVQH 120

Query: 121 DMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLFW 180
           DM ALKKFDG+YWR+LFDSRVGKTTWPYGSGVWSKKEWVLPEI+SDDIVSAFEGNSNLFW
Sbjct: 121 DMDALKKFDGQYWRSLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSDDIVSAFEGNSNLFW 180

Query: 181 AERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDT 240
           AERFGKQFLGM DLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM++PVVGVGCASTGDT
Sbjct: 181 AERFGKQFLGMTDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMHKPVVGVGCASTGDT 240

Query: 241 SAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAEL 300
           SAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCM+LIREVTAEL
Sbjct: 241 SAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMQLIREVTAEL 300

Query: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELGL 360
           PIYLANSLNSLRLEGQKTAAIEILQQFDWEVP+WVI+PGGNLGNIYAFYKGFQMC+ELGL
Sbjct: 301 PIYLANSLNSLRLEGQKTAAIEILQQFDWEVPEWVIVPGGNLGNIYAFYKGFQMCKELGL 360

Query: 361 VDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKNT 420
           VD+IPRLVCAQAANANPLYL+YKSGWK F P+KA+TTFASAIQIGDPVSIDRAVFAL+  
Sbjct: 361 VDRIPRLVCAQAANANPLYLHYKSGWKDFKPVKANTTFASAIQIGDPVSIDRAVFALQQC 420

Query: 421 GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAHG 480
            GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRNSGVI   DRTVVVSTAHG
Sbjct: 421 NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNSGVIAPTDRTVVVSTAHG 480

Query: 481 LKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPK 528
           LKFTQSKIDYHS+ I  M+CRFANPPVEVKADFG+VMDVLK +LL +  K
Sbjct: 481 LKFTQSKIDYHSKEIKDMECRFANPPVEVKADFGSVMDVLKSYLLSQNSK 518

BLAST of Tan0001461 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 1.5e-236
Identity = 418/528 (79.17%), Postives = 455/528 (86.17%), Query Frame = 0

Query: 1   MASSYFSFN---STLSPNSDSLFRRPVRRNSFL---PFTIACSSATSDPAASPVLNSSPP 60
           MASS   FN   S+L+P  D + R   R  S L   P  I+C++  ++  A       PP
Sbjct: 1   MASSCL-FNASVSSLNPKQDPIRRH--RSTSLLRHRPVVISCTADGNNIKAPIETAVKPP 60

Query: 61  LGVQNHQLAGANIQDESILCRS-VRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLL 120
              ++      NI+DE+   RS   N FSAKYVPF +   S+E YSLDEIVYRS+SGGLL
Sbjct: 61  HRTED------NIRDEARRNRSNAVNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 121 DVQHDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNS 180
           DV+HDM ALK+FDG YWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEI+ DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCAS 240
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 241 TGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREV 300
           TGDTSAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCR 360
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 361 ELGLVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFA 420
           ELGLVD+IPR+VCAQAANANPLYL+YKSGWK F P+ ASTTFASAIQIGDPVSIDRAV+A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 421 LKNTGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVS 480
           LK   GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN GVI   DRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 481 TAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHL 522
           TAHGLKFTQSKIDYHS AIP M CRF+NPPV+VKADFGAVMDVLK +L
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Tan0001461 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 2.2e-232
Identity = 406/522 (77.78%), Postives = 449/522 (86.02%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MAS     ++T  P+      +P   +S   FT+ C+SA+  PA  P     P       
Sbjct: 1   MASFSLPHSATYFPSHSETSLKP---HSAASFTVRCTSAS--PAVPPQTPQKP------R 60

Query: 61  QLAGANIQDESILCRSVRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHDMG 120
           +    NI+DE+        N SA+YVPF +   S+E YSLDEIVYRSQSG LLDVQHD  
Sbjct: 61  RSPDENIRDEARRRPHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFA 120

Query: 121 ALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLFWAER 180
           ALK++DGE+WRNLFDSRVGKT WPYGSGVWSKKEWVLPEI+ DDIVSAFEGNSNLFWAER
Sbjct: 121 ALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAER 180

Query: 181 FGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAA 240
           FGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRKMN+PV+GVGCASTGDTSAA
Sbjct: 181 FGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAA 240

Query: 241 LSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 300
           LSAYCA+AGIPSIVFLPADKIS+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIY
Sbjct: 241 LSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIY 300

Query: 301 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELGLVDK 360
           LANSLNSLRLEGQKTAAIEILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MC+ELGLVD+
Sbjct: 301 LANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDR 360

Query: 361 IPRLVCAQAANANPLYLYYKSGWKQ-FSPLKASTTFASAIQIGDPVSIDRAVFALKNTGG 420
           IPRLVCAQAANANPLYL+YKSG+K+ F+PLKA+TTFASAIQIGDPVSIDRAV+ALK + G
Sbjct: 361 IPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNG 420

Query: 421 IVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAHGLK 480
           IVEEATEEELMDATA ADSTGMFICPHTGVALTAL+KLR SGVIGANDRTVVVSTAHGLK
Sbjct: 421 IVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLK 480

Query: 481 FTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHL 522
           FTQSKIDYHS+ I  M CR ANPPV+VKA FG+VMDVLK++L
Sbjct: 481 FTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

BLAST of Tan0001461 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 9.6e-71
Identity = 155/423 (36.64%), Postives = 242/423 (57.21%), Query Frame = 0

Query: 98  YSLDEIVYRSQSGGLLDVQHDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 157
           Y +DEI+Y  + GGLL++ +D   +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 158 PEIESDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 217
           P  +   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 218 RLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVL 277
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 278 SMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGG 337
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W+VPD VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 338 NLGNIYAFYKGFQMCRELGLVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFAS 397
           N GNI A +KGF+     G++D++P++   QA  A P+   ++   K   P K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 398 AIQIGDPVSIDRAVFALKNTGGIVEEATEEELMDA-TAQADSTGMFICPHTGVALTALIK 457
           AI+IG+PV+  +A+ A+ ++GG  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 458 LRNSGVIGANDRTVVVSTAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDV 517
           L   G+I  ++R V ++T HGLK             P    R +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 518 LKD 520
           LK+
Sbjct: 432 LKE 404

BLAST of Tan0001461 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.4e-66
Identity = 155/391 (39.64%), Postives = 232/391 (59.34%), Query Frame = 0

Query: 98  YSLDEIVYR-SQSGGLLDVQHDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWV 157
           YS DE++Y  S+  GLLDV +D  ++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 158 LPEIESDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 217
             E E    V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V++ 
Sbjct: 73  PVEREP---VTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVTKA 132

Query: 218 NRLRKMNRPVVGVGCASTGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFV 277
             L  MN     V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 LEL-GMNT----VACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 278 LSMDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPG 337
           LS+  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D++VPD +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 338 GNLGNIYAFYKGFQMCRELGLVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFA 397
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG    +P +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 398 SAIQIGDPVSIDRAVFALKNTGGIVEEATEEELMDATAQ-ADSTGMFICPHTGVALTALI 457
           +AI+IG+PV+  +A+ A++ +GG  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 458 KLRNSGVIGANDRTVVVSTAHGLKFTQSKID 487
           KL + GVIG ++  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

BLAST of Tan0001461 vs. NCBI nr
Match: XP_022946725.1 (threonine synthase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 968.0 bits (2501), Expect = 3.3e-278
Identity = 480/533 (90.06%), Postives = 500/533 (93.81%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++LSP   SLFRRP+RRNS  PFTIAC SATSD AASPVL+SS PLG+QN 
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSAASPVLDSSLPLGLQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSVAADGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. NCBI nr
Match: XP_038891002.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 967.2 bits (2499), Expect = 5.7e-278
Identity = 479/534 (89.70%), Postives = 501/534 (93.82%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++ S   DSLF R  RRNS  PFT+ CSSATSD AASPVL+SSPPLG   H
Sbjct: 1   MASSLFSFNTSFSTKPDSLFHRSARRNSSSPFTVVCSSATSDSAASPVLHSSPPLG---H 60

Query: 61  QLAGANIQDES----ILCRSVRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q A ANIQD+S     +   V +NFSAKYVPFGSG+DSSEWYSLDEIVYRS+SGGLLDVQ
Sbjct: 61  QFASANIQDDSHRRCSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDEIVYRSRSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALKKFDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPV GVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVFGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKA+TTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVIGA+DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIGASDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQS 531
           GLKFTQSKIDYHSRAIP M+CR+ANPPVEVKA+ GAVMDVLK+HLL+KTPKF+S
Sbjct: 481 GLKFTQSKIDYHSRAIPGMECRYANPPVEVKAEVGAVMDVLKEHLLKKTPKFES 531

BLAST of Tan0001461 vs. NCBI nr
Match: XP_023521399.1 (threonine synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 964.5 bits (2492), Expect = 3.7e-277
Identity = 478/533 (89.68%), Postives = 499/533 (93.62%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++LSP   SLFRRP+RRN+  PFTIAC SATSD AASPVL+SS PLGVQN 
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNTSPPFTIACPSATSDSAASPVLDSSLPLGVQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
             A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  HFASVDIQDDSHRSRSVAAGGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGGYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFG+VMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGSVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. NCBI nr
Match: XP_022999212.1 (threonine synthase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 964.1 bits (2491), Expect = 4.8e-277
Identity = 479/533 (89.87%), Postives = 498/533 (93.43%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           M SS FSFN++LSP   SLFRRP+RRNS  PFTIAC SATSD  ASPVL+SS PLGVQN 
Sbjct: 1   MDSSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  QFACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. NCBI nr
Match: XP_023521397.1 (threonine synthase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 963.8 bits (2490), Expect = 6.3e-277
Identity = 477/533 (89.49%), Postives = 499/533 (93.62%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++LSP   SLFRRP+RRN+  PFTIAC SATSD AASPVL+SS P+GVQN 
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNTSPPFTIACPSATSDSAASPVLDSSLPIGVQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
             A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  HFASVDIQDDSHRSRSVAAGGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGGYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFG+VMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGSVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. ExPASy TrEMBL
Match: A0A6J1G4S3 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111450711 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.6e-278
Identity = 480/533 (90.06%), Postives = 500/533 (93.81%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++LSP   SLFRRP+RRNS  PFTIAC SATSD AASPVL+SS PLG+QN 
Sbjct: 1   MASSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSAASPVLDSSLPLGLQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  QFASVDIQDDSHRSRSVAADGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. ExPASy TrEMBL
Match: A0A6J1KA83 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111493658 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 479/533 (89.87%), Postives = 498/533 (93.43%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           M SS FSFN++LSP   SLFRRP+RRNS  PFTIAC SATSD  ASPVL+SS PLGVQN 
Sbjct: 1   MDSSLFSFNTSLSPKPSSLFRRPLRRNSSPPFTIACPSATSDSTASPVLDSSLPLGVQNP 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q A  +IQD+S   RSV      NFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ
Sbjct: 61  QFACVDIQDDSHRSRSVAGDGGRNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALK+FDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMEALKEFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGK+FLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKEFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREITAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKASTTFASAIQIGDPVSIDRAVFALKN
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           +GGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN GVI  +DRTVVVSTAH
Sbjct: 421 SGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGVIKGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKADFGAVMDVLK+HL RK PK +
Sbjct: 481 GLKFTQSKIDYHSRAIQGMECRFANPPVEVKADFGAVMDVLKEHLSRKNPKME 533

BLAST of Tan0001461 vs. ExPASy TrEMBL
Match: A0A0A0LHR0 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_2G000280 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.7e-273
Identity = 471/533 (88.37%), Postives = 494/533 (92.68%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS FSFN++LSP  DSLFRRP RRNS  PFT+ CSSATSD A S V++SS PLGV  H
Sbjct: 1   MASSLFSFNTSLSPKPDSLFRRPTRRNSSSPFTLVCSSATSDSAPSHVIDSSLPLGVHTH 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q      QD+S   RSV      NFSAKYVPFGSG+DSSEWYSLD+IVYRS+SGGLLDVQ
Sbjct: 61  Q-----FQDDSHRHRSVAVDADRNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALKKFDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGNSNLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNSNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKA+TTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           + GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN G+IG NDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGNDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKFQ 530
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKA+FGAVMDVLK+HL  K PKF+
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSGKNPKFE 528

BLAST of Tan0001461 vs. ExPASy TrEMBL
Match: A0A5A7V3M7 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G00370 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.9e-273
Identity = 469/532 (88.16%), Postives = 495/532 (93.05%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS F FN++LSP  DSL RRP RRNS  PFT+ CSSATSD A SPV++SS PLGV  H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q      QD+S   RSV     +NFSAKYVPFGSG+DSSEWYSLD+IVYRS+SGGLLDVQ
Sbjct: 61  Q-----FQDDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALKKFDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKA+TTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           + GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN G+IG +DRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKF 529
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKA+FGAVMDVLK+HL RK+PKF
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPKF 527

BLAST of Tan0001461 vs. ExPASy TrEMBL
Match: A0A1S3CB26 (Threonine synthase OS=Cucumis melo OX=3656 GN=LOC103498677 PE=3 SV=1)

HSP 1 Score: 949.5 bits (2453), Expect = 5.9e-273
Identity = 469/532 (88.16%), Postives = 495/532 (93.05%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MASS F FN++LSP  DSL RRP RRNS  PFT+ CSSATSD A SPV++SS PLGV  H
Sbjct: 1   MASSLFPFNTSLSPKPDSLLRRPTRRNSSSPFTLVCSSATSDSAPSPVIDSSLPLGVHTH 60

Query: 61  QLAGANIQDESILCRSVR----NNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQ 120
           Q      QD+S   RSV     +NFSAKYVPFGSG+DSSEWYSLD+IVYRS+SGGLLDVQ
Sbjct: 61  Q-----FQDDSHRHRSVAVDVGHNFSAKYVPFGSGYDSSEWYSLDDIVYRSRSGGLLDVQ 120

Query: 121 HDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLF 180
           H+M ALKKFDG YWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEI+S DIVSAFEGN+NLF
Sbjct: 121 HNMDALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDSSDIVSAFEGNTNLF 180

Query: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGD 240

Query: 241 TSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300
           TSAALSAYCAAAGIPSIVFLPADKIS+AQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCAAAGIPSIVFLPADKISMAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAE 300

Query: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELG 360
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGF+MC+ELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFEMCKELG 360

Query: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFALKN 420
           LVDKIPRLVCAQAANANPLYLYYKSGWK+FSPLKA+TTFASAIQIGDPVSIDRAVFAL+N
Sbjct: 361 LVDKIPRLVCAQAANANPLYLYYKSGWKEFSPLKATTTFASAIQIGDPVSIDRAVFALQN 420

Query: 421 TGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAH 480
           + GIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRN G+IG +DRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNRGIIGGSDRTVVVSTAH 480

Query: 481 GLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHLLRKTPKF 529
           GLKFTQSKIDYHSRAI  M+CRFANPPVEVKA+FGAVMDVLK+HL RK+PKF
Sbjct: 481 GLKFTQSKIDYHSRAISGMECRFANPPVEVKAEFGAVMDVLKEHLSRKSPKF 527

BLAST of Tan0001461 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 820.1 bits (2117), Expect = 1.0e-237
Identity = 418/528 (79.17%), Postives = 455/528 (86.17%), Query Frame = 0

Query: 1   MASSYFSFN---STLSPNSDSLFRRPVRRNSFL---PFTIACSSATSDPAASPVLNSSPP 60
           MASS   FN   S+L+P  D + R   R  S L   P  I+C++  ++  A       PP
Sbjct: 1   MASSCL-FNASVSSLNPKQDPIRRH--RSTSLLRHRPVVISCTADGNNIKAPIETAVKPP 60

Query: 61  LGVQNHQLAGANIQDESILCRS-VRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLL 120
              ++      NI+DE+   RS   N FSAKYVPF +   S+E YSLDEIVYRS+SGGLL
Sbjct: 61  HRTED------NIRDEARRNRSNAVNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLL 120

Query: 121 DVQHDMGALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNS 180
           DV+HDM ALK+FDG YWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEI+ DDIVSAFEGNS
Sbjct: 121 DVEHDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNS 180

Query: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCAS 240
           NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKM RPVVGVGCAS
Sbjct: 181 NLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCAS 240

Query: 241 TGDTSAALSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREV 300
           TGDTSAALSAYCA+AGIPSIVFLPA+KIS+AQLVQPIANGAFVLS+DTDFDGCMKLIRE+
Sbjct: 241 TGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREI 300

Query: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCR 360
           TAELPIYLANSLNSLRLEGQKTAAIEILQQFDW+VPDWVI+PGGNLGNIYAFYKGF+MC+
Sbjct: 301 TAELPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQ 360

Query: 361 ELGLVDKIPRLVCAQAANANPLYLYYKSGWKQFSPLKASTTFASAIQIGDPVSIDRAVFA 420
           ELGLVD+IPR+VCAQAANANPLYL+YKSGWK F P+ ASTTFASAIQIGDPVSIDRAV+A
Sbjct: 361 ELGLVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYA 420

Query: 421 LKNTGGIVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVS 480
           LK   GIVEEATEEELMDA AQADSTGMFICPHTGVALTAL KLRN GVI   DRTVVVS
Sbjct: 421 LKKCNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVS 480

Query: 481 TAHGLKFTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHL 522
           TAHGLKFTQSKIDYHS AIP M CRF+NPPV+VKADFGAVMDVLK +L
Sbjct: 481 TAHGLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

BLAST of Tan0001461 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 806.2 bits (2081), Expect = 1.6e-233
Identity = 406/522 (77.78%), Postives = 449/522 (86.02%), Query Frame = 0

Query: 1   MASSYFSFNSTLSPNSDSLFRRPVRRNSFLPFTIACSSATSDPAASPVLNSSPPLGVQNH 60
           MAS     ++T  P+      +P   +S   FT+ C+SA+  PA  P     P       
Sbjct: 1   MASFSLPHSATYFPSHSETSLKP---HSAASFTVRCTSAS--PAVPPQTPQKP------R 60

Query: 61  QLAGANIQDESILCRSVRNNFSAKYVPFGSGFDSSEWYSLDEIVYRSQSGGLLDVQHDMG 120
           +    NI+DE+        N SA+YVPF +   S+E YSLDEIVYRSQSG LLDVQHD  
Sbjct: 61  RSPDENIRDEARRRPHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGALLDVQHDFA 120

Query: 121 ALKKFDGEYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIESDDIVSAFEGNSNLFWAER 180
           ALK++DGE+WRNLFDSRVGKT WPYGSGVWSKKEWVLPEI+ DDIVSAFEGNSNLFWAER
Sbjct: 121 ALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLFWAER 180

Query: 181 FGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMNRPVVGVGCASTGDTSAA 240
           FGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRKMN+PV+GVGCASTGDTSAA
Sbjct: 181 FGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGCASTGDTSAA 240

Query: 241 LSAYCAAAGIPSIVFLPADKISIAQLVQPIANGAFVLSMDTDFDGCMKLIREVTAELPIY 300
           LSAYCA+AGIPSIVFLPADKIS+AQLVQPIANGAFVLS+DTDFDGCM LIREVTAELPIY
Sbjct: 241 LSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIREVTAELPIY 300

Query: 301 LANSLNSLRLEGQKTAAIEILQQFDWEVPDWVIIPGGNLGNIYAFYKGFQMCRELGLVDK 360
           LANSLNSLRLEGQKTAAIEILQQF+W+VPDWVI+PGGNLGNIYAFYKGF MC+ELGLVD+
Sbjct: 301 LANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHMCKELGLVDR 360

Query: 361 IPRLVCAQAANANPLYLYYKSGWKQ-FSPLKASTTFASAIQIGDPVSIDRAVFALKNTGG 420
           IPRLVCAQAANANPLYL+YKSG+K+ F+PLKA+TTFASAIQIGDPVSIDRAV+ALK + G
Sbjct: 361 IPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRAVYALKKSNG 420

Query: 421 IVEEATEEELMDATAQADSTGMFICPHTGVALTALIKLRNSGVIGANDRTVVVSTAHGLK 480
           IVEEATEEELMDATA ADSTGMFICPHTGVALTAL+KLR SGVIGANDRTVVVSTAHGLK
Sbjct: 421 IVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTVVVSTAHGLK 480

Query: 481 FTQSKIDYHSRAIPSMDCRFANPPVEVKADFGAVMDVLKDHL 522
           FTQSKIDYHS+ I  M CR ANPPV+VKA FG+VMDVLK++L
Sbjct: 481 FTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511