Tan0001219 (gene) Snake gourd v1

BLAST of Tan0001219 vs. ExPASy Swiss-Prot
Match: Q9LQV2 (RNA-dependent RNA polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=RDR1 PE=2 SV=1)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 721/1133 (63.64%), Postives = 859/1133 (75.82%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+T+ + GFP+ V+A+ VK FLE  TG GTVYAIKVR PK+GG RVYAIVQFT      
Sbjct: 1    MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             II+ A  RL+YG SYLKA   + DIVPKPR  +HT+  L + FGCQVS ++F  LW   
Sbjct: 61   LIITAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWSAQ 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
               V+FGIGMRK++F       +YRLELSYENIWQI LH PQ +S K+L+IQ+ GAP+I+
Sbjct: 121  DVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPKIF 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            +K       +F    ++F+ +  D+QW+RT DFT S  IGQS++ CL+LP    +P+FR+
Sbjct: 181  EKEDQPINLLFG--IMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRE 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKD-LVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGP 300
            NFA Y E      L++ G  +S + + LVP+VD  P   LP+EI+FK+N LVQN C+ GP
Sbjct: 241  NFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGP 300

Query: 301  LLDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKY--SRHPPNSPAI 360
             LD  FYRL++  +     ++H LEKLFHL EC YEP+ +L ++Y+K+      P SP I
Sbjct: 301  ALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTI 360

Query: 361  SLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSID 420
            SLD GLVY+ RVQ+TP +VYF GPEVNVSNRVLR + + I+NFLRV+FVDE+ +K+RS+D
Sbjct: 361  SLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMD 420

Query: 421  LLPRMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLD 480
            L PR S+     +T +Y RI SVLR+GIVIG K FEFLAFSSSQLR+NS WMFA    + 
Sbjct: 421  LSPRSST---QRRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRIT 480

Query: 481  AAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFS 540
            AA IR WMGDF HI+N AKYAARLGQSF SS ETL+V   E E+IPD+E+     +YVFS
Sbjct: 481  AAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFS 540

Query: 541  DGIGKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFES 600
            DGIGKIS++FA++VA KC L  F+PSAFQIRYGGYKGVVAVDP SS KLSLR SM KFES
Sbjct: 541  DGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFES 600

Query: 601  DNTKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEA 660
            +NTKLDVL +SKYQPC++NRQ+ITLLSTLGV D VFEKKQRE V++LDAILT PL A EA
Sbjct: 601  ENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEA 660

Query: 661  LELMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMG 720
            L LM+PGENTNILK ++ CGYKPD EP+LSMMLQ FRASKLLELRTK+RIFI  GR++MG
Sbjct: 661  LGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMG 720

Query: 721  CLDETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVR 780
            CLDET TLEYGQV VQ S              MR   R  +I G V+VAKNPCLHPGDVR
Sbjct: 721  CLDETRTLEYGQVVVQYSDP------------MRPGRR-FIITGPVVVAKNPCLHPGDVR 780

Query: 781  VLRAVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDY 840
            VL+AVN+P L HMVDCVVFPQKG RPHPNECSGSDLDGDIYFVCWD EL+PPR   PMDY
Sbjct: 781  VLQAVNVPALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDY 840

Query: 841  TPAPPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLF 900
            TP P   LD DV IE+V+EYF NY+VNDSLGIIANAHTAFADKE  KA S PC+ELAK F
Sbjct: 841  TPEPTQILDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKF 900

Query: 901  SIAVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIR 960
            S AVDFPKTGV A+IP HL VKE+PDFMEKPD+P+YESKNVIGKLFR VK+ AP +  I+
Sbjct: 901  STAVDFPKTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIK 960

Query: 961  SFTRDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMR 1020
            SFT DVA + YD DMEV+GFE+YV++AFY K NYD+KLGNL+DYYGIK+EAEILSG IMR
Sbjct: 961  SFTLDVASKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMR 1020

Query: 1021 MSKSFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYW 1080
            MSKSFT+RRDAE+I  AVR+LRKE  + FNA E       ++  AKASAWYHVTYH SYW
Sbjct: 1021 MSKSFTKRRDAESIGRAVRALRKETLSLFNASEE-----EENESAKASAWYHVTYHSSYW 1080

Query: 1081 GCYNEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLG 1131
            G YNEG  RDH+LSF WCVYDKL++IK+ NL RR+   R  TL+R   VL  G
Sbjct: 1081 GLYNEGLNRDHFLSFAWCVYDKLVRIKKTNLGRRQ---RQETLERLDHVLRFG 1107

BLAST of Tan0001219 vs. ExPASy Swiss-Prot
Match: Q0DXS3 (Probable RNA-dependent RNA polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR1 PE=2 SV=2)

HSP 1 Score: 972.6 bits (2513), Expect = 3.8e-282
Identity = 482/731 (65.94%), Postives = 581/731 (79.48%), Query Frame = 0

Query: 376  VYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLPRMSSTIKDGKTDIYS 435
            VYF GPE+NVSNRV+R F  DI+NFLR++FVDE+ +K+R+ DL PR +S     +T +Y 
Sbjct: 9    VYFYGPEINVSNRVVRNFSSDIENFLRISFVDEDCEKLRATDLSPRSASGHDANRTALYK 68

Query: 436  RILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAAIREWMGDFRHIKNPA 495
            R+LSVL +GI IG KNFEFLAFSSSQLRDNS WMFASR GL A+ IR WMGDFR+I+N A
Sbjct: 69   RVLSVLSDGITIGGKNFEFLAFSSSQLRDNSAWMFASRQGLAASDIRTWMGDFRNIRNVA 128

Query: 496  KYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGIGKISSDFAKRVAAKC 555
            KYAARLGQSF SSTETL V ++E E I DI+      ++VFSDGIGKISS FA  VA KC
Sbjct: 129  KYAARLGQSFSSSTETLKVQKYEVEEISDIK---NGTQHVFSDGIGKISSAFANEVAMKC 188

Query: 556  SLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNTKLDVLGYSKYQPCFL 615
            +L+ F PSAFQIRYGGYKGVVAVDP S  KLSLR SM KF+SDN  +DVL YSKYQP FL
Sbjct: 189  NLKRFAPSAFQIRYGGYKGVVAVDPTSRWKLSLRKSMLKFQSDNITVDVLAYSKYQPGFL 248

Query: 616  NRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALELMSPGENTNILKEMLK 675
            NRQ+ITLLSTLGV+D VFE+KQ EAV QL+ ++TDP  A EA+ELM  GE TN +KE+L 
Sbjct: 249  NRQLITLLSTLGVRDSVFEQKQEEAVNQLNKMVTDPQAAIEAIELMPMGEITNAVKELLL 308

Query: 676  CGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLDETGTLEYGQVFVQIS 735
            CGY+PD EPYLSM+LQTFRASKLLEL+TKSRI IP GRA+MGCLDET TL+YGQVF++ +
Sbjct: 309  CGYQPDDEPYLSMLLQTFRASKLLELKTKSRILIPKGRAMMGCLDETRTLKYGQVFIRAT 368

Query: 736  SARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLRAVNIPRLYHMVDCVV 795
            S  + N  D F            + G V++AKNPCLHPGD+R+L AV++P L+HM +CVV
Sbjct: 369  SGVNDN--DRF-----------TVTGKVVIAKNPCLHPGDIRILHAVDVPVLHHMFNCVV 428

Query: 796  FPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPAPPIQLDRDVRIEDVQ 855
            FPQ+G RPHPNECSGSDLDGDIYFV WD  LIPPR V PMDYTPAP   LD DV IE+V+
Sbjct: 429  FPQQGPRPHPNECSGSDLDGDIYFVSWDPSLIPPRMVTPMDYTPAPTETLDHDVTIEEVE 488

Query: 856  EYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIAVDFPKTGVPAIIPSH 915
            EYF NY+VN+SLG+IANAH  FADKE  KA SSPC+ELAKLFSIAVDFPKTGVPA+IP  
Sbjct: 489  EYFTNYIVNESLGMIANAHVVFADKEDLKAESSPCIELAKLFSIAVDFPKTGVPALIPPE 548

Query: 916  LNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFTRDVAMQCYDFDMEVE 975
            L+VKE+PDFMEK D+ +YESK VIGKL+R +K   P   +I+ FTR+VA + YD DM V+
Sbjct: 549  LHVKEYPDFMEKLDKVTYESKGVIGKLYREIKKHTP---HIKHFTREVARRSYDTDMIVD 608

Query: 976  GFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSKSFTRRRDAEAINLAV 1035
            G+EDY+ +A   K+ YD+KLGNL+D+YGIKSEAEI+SG I++M+K+FT++ DA+AI LAV
Sbjct: 609  GYEDYITEAMALKDEYDFKLGNLMDHYGIKSEAEIISGCILKMAKNFTKKSDADAIRLAV 668

Query: 1036 RSLRKEARTWFNAKESGPDS-GSDDLFAKASAWYHVTYHHSYWGCYNEGTKRDHYLSFPW 1095
            RSLRKEAR+ F+      +  G D   AKASAWYHVTYH  +WGCYNEG +R H++SFPW
Sbjct: 669  RSLRKEARSRFSEMSLDDNGHGHDASEAKASAWYHVTYHPEFWGCYNEGYERPHFISFPW 720

Query: 1096 CVYDKLMQIKE 1106
            C+Y+KL++IK+
Sbjct: 729  CIYEKLLRIKQ 720

BLAST of Tan0001219 vs. ExPASy Swiss-Prot
Match: O82504 (RNA-dependent RNA polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=RDR2 PE=1 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 1.7e-218
Identity = 450/1133 (39.72%), Postives = 661/1133 (58.34%), Query Frame = 0

Query: 4    TVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGG--TRVYAIVQFTG-AAQAE 63
            TV I+  P  + AD +  FLE H G  TV+A+++ P  R     R +A VQFT    ++ 
Sbjct: 11   TVKISNVPQTIVADELLRFLELHLGEDTVFALEI-PTTRDNWKPRDFARVQFTTLEVKSR 70

Query: 64   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGE-RFHVL--W 123
            + +  +  +L + +  L+   A  DI+P+P      L+++ L  G   S E RF  L  W
Sbjct: 71   AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKW 130

Query: 124  EGGVDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQ--LHHPQHQSMKYLLIQLYG 183
            +G    +      R++ F +  +   Y++E+ +E+I +      +     +   L++L  
Sbjct: 131  DGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKY 190

Query: 184  APRIYKKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQL 243
             P+++K+V       F      F KE  D  W+RT DF+ S SIG S+  CL++ NG  +
Sbjct: 191  GPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTM 250

Query: 244  PNFRQNFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSR-PNVFLPYEIIFKVNVLVQNG 303
             +      YY E       VD G  F+ +  +VP++++    +  PYEI+F++N LV   
Sbjct: 251  LDIFSGLPYYREDTLSLTYVD-GKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQ 310

Query: 304  CIPGPLLDTGFYRLVDPSR-IRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPN 363
             I   L       L+   R + +E     L+KL       Y+P  F+  Q +   +   +
Sbjct: 311  KI--SLFAASDMELIKILRGMSLETALVILKKLHQQSSICYDPVFFVKTQMQSVVKKMKH 370

Query: 364  SPAIS----LDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEE 423
            SPA +     +  ++  +R  +TP K+Y  GPE+  +N V++ F + + +F+RVTFV+E+
Sbjct: 371  SPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEED 430

Query: 424  WDKM--RSIDLLPRMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSL 483
            W K+   ++ +  +    +K  +T+IY+R+LS+L  GI +G K FEFLAFS+SQLR NS+
Sbjct: 431  WSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGNSV 490

Query: 484  WMFASRPGLDAAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEV 543
            WMFAS   + A  IREWMG FR I++ +K AAR+GQ F +S +TL V   + E IPDIEV
Sbjct: 491  WMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDIEV 550

Query: 544  QHGEVKYVFSDGIGKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLS 603
                  Y FSDGIGKIS  FAK+VA KC L +  PSAFQIRYGGYKGV+AVD  S  KLS
Sbjct: 551  TTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLS 610

Query: 604  LRNSMCKFESDNTKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAI 663
            LR+SM KF+S+N  L+V  +++  PCFLNR++I LLSTLG++D +FE  Q   +  L  +
Sbjct: 611  LRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNM 670

Query: 664  LTDPLRAQEALELMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRI 723
            L D   A   L+ +S   + N+L +ML  GY P  EPYLSMML+    S+L EL+++ RI
Sbjct: 671  LEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRCRI 730

Query: 724  FIPDGRAVMGCLDETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAK 783
             +P GR ++GC+DE G LEYGQV+V+++  +    S   ++  +  E   V+ G V+V K
Sbjct: 731  LVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVVTK 790

Query: 784  NPCLHPGDVRVLRAVNIPRLYH--MVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTE 843
            NPCLHPGD+RVL A+          +DC++FPQKG RPHPNECSG DLDGD +FV WD +
Sbjct: 791  NPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWDEK 850

Query: 844  LIPPRQVLPMDYTPAPPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKA 903
            +IP     PMDY  + P  +D DV +E++ ++FV+YM++D+LG+I+ AH   AD++  KA
Sbjct: 851  IIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPEKA 910

Query: 904  RSSPCLELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRA 963
            RS  CLELA L S AVDF KTG PA +P  L  +EFPDF+E+ ++P+Y S++V GKL+RA
Sbjct: 911  RSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLYRA 970

Query: 964  VKDIAPTVSYIRSFTRDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIK 1023
            VK    +++  +          YD  +E  GFE ++E A  H++ Y  KL +L+ YYG  
Sbjct: 971  VKS---SLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYGAA 1030

Query: 1024 SEAEILSGSI----MRMSKSFTRRRD-AEAINLAVRSLRKEARTWFNAKESGPDSGSDDL 1083
            +E EIL+G +    M +++   R  D  + I L+V+ L KEA  WF        S  D+ 
Sbjct: 1031 NEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFE------KSCEDEQ 1090

Query: 1084 FAK--ASAWYHVTYHHSYWGCYNEGTKRDHYLSFPWCVYDKLMQIKENNLRRR 1112
              K  ASAWY+VTY+       N   ++  +LSFPW V D L+ IK  N +R+
Sbjct: 1091 QKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIKAENAQRQ 1121

BLAST of Tan0001219 vs. ExPASy Swiss-Prot
Match: Q7XM31 (Probable RNA-dependent RNA polymerase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=RDR2 PE=2 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 8.9e-215
Identity = 443/1131 (39.17%), Postives = 650/1131 (57.47%), Query Frame = 0

Query: 11   PSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGG-TRVYAIVQFTGAAQAESIISLAN-- 70
            PS V A+ +  F       G  +A ++    RG  +R +  VQF  AA A +   LA+  
Sbjct: 19   PSAVAAELLAFFDSAVAVAGGAFACEIAAAHRGWLSRGHGTVQFGSAAAAAAAAGLASSG 78

Query: 71   RRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHV--LWEG-GVDLV 130
            R   +  + L    + VD++P+            L  G +V+   F     W+G   +++
Sbjct: 79   RLPRFLGALLSVSPSPVDLLPRASDLSLRAAGAGLVVGDRVAERVFEAADAWDGVRAEVI 138

Query: 131  TFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIYKKVA 190
                G R+++  L H+   Y+LE+ +E++            M  +L+QL  APRI+  ++
Sbjct: 139  P---GKRRVDLYLEHDSQRYKLEVLFEDMKDCL--GCTLDGMGAILLQLNYAPRIHTAIS 198

Query: 191  -PSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQNFA 250
             P+    F D   +  KE     WVR  DFTP+ S G+ S+L LKL     + +  ++  
Sbjct: 199  GPAVNSRFMDDRFHACKEDAKFSWVRALDFTPNYSFGRCSTLVLKLGKSALVSDILKSLP 258

Query: 251  YYEEFENEFEL-VDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPLLD 310
            +     N  EL ++  DG   S ++VP+V    +  +PYE++F++N L+  G I    ++
Sbjct: 259  F---SGNLGELTMNSMDGVGASSNVVPLVHCPRDYSVPYEVLFRLNSLMHMGKIVAKHVN 318

Query: 311  TGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLDA- 370
               ++ +    + ++      EK+  L+   Y P  F+  Q   YS    ++  +S +  
Sbjct: 319  ADLFKAL--QELPVDVSRRIFEKMHKLESTCYGPLQFI--QQEAYSMKRSHNVLLSNEGE 378

Query: 371  -----GLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSI 430
                  L+   RV ITP K++  GPE  V+N V++       +F+RVTFVDE+W K+ S 
Sbjct: 379  GEGERKLMKCYRVNITPSKIFCFGPEEEVTNYVVKHHSAYASDFVRVTFVDEDWSKLSSN 438

Query: 431  DLLPRMSSTI--KDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRP 490
             +  R+      K  KT +Y RILS+L+ G  IG KNFEFLAFS+SQLR NS+WMFAS  
Sbjct: 439  AISARIEQGFFSKPFKTGLYYRILSILKKGFSIGPKNFEFLAFSASQLRGNSVWMFASNA 498

Query: 491  GLDAAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKY 550
             L+A  IR WMG F +I++ +K AAR+GQ F SS +T  V R + E+IPDIE+     KY
Sbjct: 499  SLNAGGIRRWMGHFENIRSVSKCAARMGQLFSSSRQTFEVLRWDVEVIPDIEITTDGSKY 558

Query: 551  VFSDGIGKISSDFAKRVAAKCSLQAFT-PSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMC 610
            +FSDGIGKIS  FAKRVA    L     PSAFQIRYGGYKGV+A+DP+SSI LSLR SM 
Sbjct: 559  IFSDGIGKISLRFAKRVAHHVGLDPTNLPSAFQIRYGGYKGVIAIDPMSSIDLSLRPSMK 618

Query: 611  KFESDNTKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLR 670
            KFES++  L++  +SK QPC++NR++I+LLSTLG++DE+F   Q++ + + + +LT+   
Sbjct: 619  KFESESRMLNITSWSKSQPCYVNREIISLLSTLGIRDEIFVAMQQDEMRETEEMLTNKEV 678

Query: 671  AQEALELMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGR 730
            A   L  +  G  T    +ML  GY+P  EPYLSM+L+  + ++L ++RT+ +I +P GR
Sbjct: 679  ALSVLGKLG-GSETKTAVKMLLQGYEPSSEPYLSMILKAHQENRLTDIRTRCKIHVPKGR 738

Query: 731  AVMGCLDETGTLEYGQVFVQI--SSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCL 790
             ++GCLDETG LEYGQV+++I  +S   ++ + ++ +N  G    +V  G V + KNPCL
Sbjct: 739  VLIGCLDETGVLEYGQVYIRITKNSKEQKDSNQSYFYNDDGKTATVV--GKVAITKNPCL 798

Query: 791  HPGDVRVLRAVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ 850
            HPGD+RVL A+  P L  MVDC+VFPQ+G RPHPNECSG DLDGD+YF+ WD +LIP + 
Sbjct: 799  HPGDIRVLEAIYDPDLVGMVDCLVFPQRGERPHPNECSGGDLDGDLYFITWDDKLIPEKV 858

Query: 851  VLPMDYTPAPPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCL 910
              PMDYT   P  +D  V +E++Q++FV+YM+NDSLG I+ AH   AD+   KARS  CL
Sbjct: 859  DTPMDYTATRPRIMDHVVTLEEIQKHFVDYMINDSLGAISTAHLIHADRSPLKARSPECL 918

Query: 911  ELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAP 970
            +LA L S+AVDF KTG PA +P  L  +E+PDFME+ ++P Y S  V+GKL+R+      
Sbjct: 919  QLATLHSMAVDFAKTGAPAEMPRTLRPREYPDFMERWEKPMYISNGVLGKLYRSAMGHME 978

Query: 971  TVSYIRSFTRDVAM--QCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAE 1030
                  + +   A     YD D+EV G +++++ A  +   Y+ KL  L++YY  + E E
Sbjct: 979  KSGDSGALSSSSAQPSPTYDPDLEVPGSDEFLQAAEEYYELYEEKLTTLMNYYRAELEDE 1038

Query: 1031 ILSGSIMRMSKSFTRRRD-------AEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFA 1090
            IL+G+I   +K    +RD        + I  AV +L +EAR W  +         +D   
Sbjct: 1039 ILTGNI--RNKMLYLKRDNKRYFEMKDRIVAAVDALHREARGWLLSSRK-----EEDASR 1098

Query: 1091 KASAWYHVTYHHSYWGCYNEGTKRDHYLSFPWCVYDKLMQIKENNLRRRER 1114
             ASAWY VTYH        +  +   + SFPW   D L+ IK ++  RR R
Sbjct: 1099 MASAWYRVTYH-------PDRRRGKRFWSFPWIACDNLLAIKASSQLRRRR 1120

BLAST of Tan0001219 vs. ExPASy Swiss-Prot
Match: Q9SG02 (RNA-dependent RNA polymerase 6 OS=Arabidopsis thaliana OX=3702 GN=RDR6 PE=1 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 1.2e-166
Identity = 401/1102 (36.39%), Postives = 574/1102 (52.09%), Query Frame = 0

Query: 92   TYVHTLENLTLCFGCQVSGERFHVLWEG-GVDLV------TFGIGMRK-----MNFCLIH 151
            T  + L  +TL  G  VS + F V W   GVD +      T     RK         ++H
Sbjct: 127  TVPYKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMH 186

Query: 152  NFV--EYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIYKKVAPSS------GQVF 211
              +  +Y+LEL   +I  ++ +   H  +  L++QL  +PR++ + A         G + 
Sbjct: 187  AVINCDYKLELLVRDIQTVRQYKTLHGFV--LILQLASSPRVWYRTADDDIYDTVPGDLL 246

Query: 212  DDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKL---------------------- 271
            DD          DD W+RT DFT   +IG+  S  + +                      
Sbjct: 247  DD----------DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQE 306

Query: 272  PNGRQLPNFRQNFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNV 331
               R  P  R    + E   + F  +   +G S                  +EI+F VN 
Sbjct: 307  ERVRWPPRIRNEPCFGEPVSDHFFCIHHKEGIS------------------FEIMFLVNS 366

Query: 332  LVQNGCIPGPLLDTGFYRLV--DPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRK- 391
            ++  G      L   F+ L+   P  + I  ++H          C+Y+   F  + Y++ 
Sbjct: 367  VLHRGVFNQFQLTERFFDLLRNQPKDVNIASLKHL---------CTYKRPVF--DAYKRL 426

Query: 392  ------YSRHPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNF 451
                    ++P    +      +  +RR+ ITP + Y   PEV +SNRVLR++    + F
Sbjct: 427  KLVQEWIQKNPKLLGSHEQSEDISEIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERF 486

Query: 452  LRVTFVDEEWDKMRSIDLLPRMSSTIKD-------GKTDIYSRILSVLRNGIVIGAKNFE 511
            LRVTF+DE    + S  L   ++  +KD        KT ++ R+ S+L +G  +  + + 
Sbjct: 487  LRVTFMDESMQTINSNVLSYFVAPIVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYS 546

Query: 512  FLAFSSSQLRDNSLWMFASRPGLDAAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLS 571
            FLAFS++QLRD S W FA       + I+ WMG F+  KN AK AAR+G  F S+  T+ 
Sbjct: 547  FLAFSANQLRDRSAWFFAEDGKTRVSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVD 606

Query: 572  VARHEREI-IPDIEVQHGEVKYVFSDGIGKISSDFAKRVAAKCSLQA-FTPSAFQIRYGG 631
            V  HE +  +PDIE ++G   YVFSDGIG I+ D A  V  K  L   ++P A+QIRY G
Sbjct: 607  VMPHEVDTEVPDIE-RNG---YVFSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAG 666

Query: 632  YKGVVAVDPLSS--IKLSLRNSMCKFESDNTKLDVLGYSKYQPCFLNRQVITLLSTLGVK 691
            +KGVVA  P  S  I+L+LR+SM KF S +T L++  ++++QP FLNRQ+ITLLS LGV 
Sbjct: 667  FKGVVARWPSKSDGIRLALRDSMKKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVP 726

Query: 692  DEVFEKKQREAVEQLDAILTDPLRAQEALELMSPGENTNILKEMLKCGYKPDIEPYLSMM 751
            DE+F   Q   + +L+ IL D   A E L   S  E  N    ML  G+KP  EP+L  M
Sbjct: 727  DEIFWDMQESMLYKLNRILDDTDVAFEVL-TASCAEQGNTAAIMLSAGFKPKTEPHLRGM 786

Query: 752  LQTFRASKLLELRTKSRIFIPDGRAVMGCLDETGTLEYGQVFVQISSARHRNLSDTFAFN 811
            L + R ++L  LR KSRIF+  GR +MGCLDE G LE+GQ F+Q+S     N        
Sbjct: 787  LSSVRIAQLWGLREKSRIFVTSGRWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSR 846

Query: 812  MRGSERCL-VIEGNVIVAKNPCLHPGDVRVLRAVNIPRLYHMVDCVVFPQKGSRPHPNEC 871
             + ++  L V++G V +AKNPCLHPGDVR+L AV++P+L+HM DC++FPQKG RPH NE 
Sbjct: 847  FKETKTDLEVVKGYVAIAKNPCLHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEA 906

Query: 872  SGSDLDGDIYFVCWDTELIPP-RQVLP-MDYTPAPPIQLDRDVRIEDVQEYFVNYMVNDS 931
            SGSDLDGD+YFV WD +LIPP R+  P M Y  A    L R V  +D+ ++F   + N+ 
Sbjct: 907  SGSDLDGDLYFVAWDQKLIPPNRKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQ 966

Query: 932  LGIIANAHTAFADKESFKARSSPCLELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDFME 991
            LG I NAH   AD+  + A    CL LA+L + AVDFPKTG    +P HL  K +PDFM 
Sbjct: 967  LGTICNAHVVHADRSEYGAMDEECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMG 1026

Query: 992  KPDRPSYESKNVIGKLFRAVKDIAPTVSYIRS-FTRDVAMQCYDFDMEVEGFEDYVEDAF 1051
            K D  +Y+S  ++G+L+R VK++    +   S  + D +   YD  +E+ GFED + +A+
Sbjct: 1027 KEDYQTYKSNKILGRLYRRVKEVYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAW 1086

Query: 1052 YHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSKSFTRRRD--AEAINLAVRSLRKEAR 1111
             HK  YD +L  LL  Y ++ E EI++G I  M K  ++++    E +  +  SL+KE R
Sbjct: 1087 GHKCLYDGQLIGLLGQYKVQKEEEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFR 1146

Query: 1112 TWFNAKESGPDSGSDD-----LFAKASAWYHVTYHHSYWGCYNEGTKRDH-----YLSFP 1116
              F       ++ S++        KASAWYHVTYH  +     E    D       LSF 
Sbjct: 1147 KVFEETIPDHENLSEEEKNILYEKKASAWYHVTYHPEWVKKSLELQDPDESSHAAMLSFA 1178

BLAST of Tan0001219 vs. NCBI nr
Match: KAG6599933.1 (RNA-dependent RNA polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2072.7 bits (5369), Expect = 0.0e+00
Identity = 1004/1132 (88.69%), Postives = 1066/1132 (94.17%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+G+VYAIKVRPPK GG+RVYAIVQF GAAQAE
Sbjct: 988  MGKTVHISGFPSHATADSVKSFLELRTGLGSVYAIKVRPPKGGGSRVYAIVQFIGAAQAE 1047

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLE+LTLCFGCQ+S E+FHVLWEG 
Sbjct: 1048 LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQISSEKFHVLWEGD 1107

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 1108 VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 1167

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQWVRT DFTPS SIGQSSSLCLKLPNGRQLPNFR+
Sbjct: 1168 KKVAPSSGQIFDDPLFNFFKEVPDDQWVRTADFTPSNSIGQSSSLCLKLPNGRQLPNFRR 1227

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+GFSCS DLVP+VDSRPNV LPYEIIFKVNVLVQNGCIPGP 
Sbjct: 1228 HFAYYEEFEDEFKLVDGGNGFSCSDDLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIPGPS 1287

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSR+R+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 1288 LDTGFYRLVDPSRMRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 1347

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
            AGLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 1348 AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 1407

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 1408 RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 1467

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDF+HIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 1468 IREWMGDFQHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 1527

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 1528 GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 1587

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 1588 KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 1647

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 1648 MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 1707

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQISSARHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 1708 ETGTLEYGQVFVQISSARHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 1767

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 1768 AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 1827

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 1828 PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 1887

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 1888 VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFT 1947

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 1948 RDVAMRCYDSDMETEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 2007

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESGPDSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 2008 SFTRRRDAEAINLAARSLRKEARTWFNTKESGPDSGSDDLFAKASAWYHVTYHHSYWGSY 2067

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLRRRE+ASR+ATLDRF  VLNL GR
Sbjct: 2068 NDGMKRDHYLSFPWCVYHKLMQVKENNLRRREKASRLATLDRFSRVLNLDGR 2118

BLAST of Tan0001219 vs. NCBI nr
Match: XP_022943173.1 (RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943174.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata] >XP_022943175.1 RNA-dependent RNA polymerase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1004/1132 (88.69%), Postives = 1063/1132 (93.90%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQF GAAQAE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLE+LTLCFGCQVS E+FHVLWEG 
Sbjct: 61   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLWEGD 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 121  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQWVRT DFTPS S+GQSSSLCLKLPNGRQLPNFR+
Sbjct: 181  KKVAPSSGQIFDDPLFNFFKEVPDDQWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRR 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+ FSCS DLVP+VDSRPNV LPYEIIFKVNVLVQNGCI GP 
Sbjct: 241  HFAYYEEFEDEFKLVDGGNSFSCSDDLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPS 300

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 301  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 360

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
             GLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 361  DGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 420

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 421  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 480

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 541  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 600

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 601  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 660

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 661  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 720

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQISS RHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 721  ETGTLEYGQVFVQISSVRHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 780

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 781  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 840

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 841  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 900

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFT 960

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 961  RDVAMRCYDSDMETEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1020

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESGPDSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1021 SFTRRRDAEAINLAARSLRKEARTWFNTKESGPDSGSDDLFAKASAWYHVTYHHSYWGSY 1080

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLRRRE+ASR+ATLDRF  VLNL GR
Sbjct: 1081 NDGMKRDHYLSFPWCVYHKLMQVKENNLRRREKASRLATLDRFSRVLNLDGR 1131

BLAST of Tan0001219 vs. NCBI nr
Match: XP_022943172.1 (RNA-dependent RNA polymerase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1004/1132 (88.69%), Postives = 1063/1132 (93.90%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQF GAAQAE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLE+LTLCFGCQVS E+FHVLWEG 
Sbjct: 75   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLWEGD 134

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 135  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 194

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQWVRT DFTPS S+GQSSSLCLKLPNGRQLPNFR+
Sbjct: 195  KKVAPSSGQIFDDPLFNFFKEVPDDQWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRR 254

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+ FSCS DLVP+VDSRPNV LPYEIIFKVNVLVQNGCI GP 
Sbjct: 255  HFAYYEEFEDEFKLVDGGNSFSCSDDLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPS 314

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 315  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 374

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
             GLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 375  DGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 434

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 435  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 494

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 495  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 554

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 555  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 614

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 615  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 674

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 675  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 734

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQISS RHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 735  ETGTLEYGQVFVQISSVRHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 794

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 795  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 854

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 855  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 914

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 915  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFT 974

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 975  RDVAMRCYDSDMETEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1034

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESGPDSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1035 SFTRRRDAEAINLAARSLRKEARTWFNTKESGPDSGSDDLFAKASAWYHVTYHHSYWGSY 1094

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLRRRE+ASR+ATLDRF  VLNL GR
Sbjct: 1095 NDGMKRDHYLSFPWCVYHKLMQVKENNLRRREKASRLATLDRFSRVLNLDGR 1145

BLAST of Tan0001219 vs. NCBI nr
Match: XP_022994928.1 (probable RNA-dependent RNA polymerase 1 isoform X2 [Cucurbita maxima] >XP_022994935.1 probable RNA-dependent RNA polymerase 1 isoform X2 [Cucurbita maxima] >XP_022994937.1 probable RNA-dependent RNA polymerase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1005/1132 (88.78%), Postives = 1064/1132 (93.99%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQFTGAAQAE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLENLTLCFGCQVS E+FHVLWEG 
Sbjct: 61   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLWEGD 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 121  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQ VRT DFTPS SIGQSSSLCLKLPNGRQLPNFR+
Sbjct: 181  KKVAPSSGQIFDDPLFNFFKEVPDDQCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRR 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+GFSCS DLVP+VDS PNV LPYEIIFKVNVLVQNGCIPGP 
Sbjct: 241  HFAYYEEFEDEFKLVDGGNGFSCSDDLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPS 300

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 301  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 360

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
            AGLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 420

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 421  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 480

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDF+HIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 481  IREWMGDFQHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 541  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 600

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 601  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 660

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 661  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 720

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQIS+ARHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 721  ETGTLEYGQVFVQISNARHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 780

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 781  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 840

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 841  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 900

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDR SYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFT 960

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 961  RDVAMRCYDSDMEAEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1020

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESG DSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1021 SFTRRRDAEAINLAARSLRKEARTWFNTKESGLDSGSDDLFAKASAWYHVTYHHSYWGSY 1080

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLR+RERASR+ATLDRF  VLNL GR
Sbjct: 1081 NDGMKRDHYLSFPWCVYHKLMQVKENNLRKRERASRLATLDRFSRVLNLDGR 1131

BLAST of Tan0001219 vs. NCBI nr
Match: XP_022994920.1 (probable RNA-dependent RNA polymerase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1005/1132 (88.78%), Postives = 1064/1132 (93.99%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQFTGAAQAE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 74

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLENLTLCFGCQVS E+FHVLWEG 
Sbjct: 75   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLWEGD 134

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 135  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 194

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQ VRT DFTPS SIGQSSSLCLKLPNGRQLPNFR+
Sbjct: 195  KKVAPSSGQIFDDPLFNFFKEVPDDQCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRR 254

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+GFSCS DLVP+VDS PNV LPYEIIFKVNVLVQNGCIPGP 
Sbjct: 255  HFAYYEEFEDEFKLVDGGNGFSCSDDLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPS 314

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 315  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 374

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
            AGLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 375  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 434

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 435  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 494

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDF+HIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 495  IREWMGDFQHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 554

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 555  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 614

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 615  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 674

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 675  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 734

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQIS+ARHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 735  ETGTLEYGQVFVQISNARHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 794

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 795  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 854

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 855  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 914

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDR SYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 915  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFT 974

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 975  RDVAMRCYDSDMEAEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1034

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESG DSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1035 SFTRRRDAEAINLAARSLRKEARTWFNTKESGLDSGSDDLFAKASAWYHVTYHHSYWGSY 1094

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLR+RERASR+ATLDRF  VLNL GR
Sbjct: 1095 NDGMKRDHYLSFPWCVYHKLMQVKENNLRKRERASRLATLDRFSRVLNLDGR 1145

BLAST of Tan0001219 vs. ExPASy TrEMBL
Match: A0A6J1FXH5 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1004/1132 (88.69%), Postives = 1063/1132 (93.90%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQF GAAQAE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLE+LTLCFGCQVS E+FHVLWEG 
Sbjct: 61   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLWEGD 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 121  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQWVRT DFTPS S+GQSSSLCLKLPNGRQLPNFR+
Sbjct: 181  KKVAPSSGQIFDDPLFNFFKEVPDDQWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRR 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+ FSCS DLVP+VDSRPNV LPYEIIFKVNVLVQNGCI GP 
Sbjct: 241  HFAYYEEFEDEFKLVDGGNSFSCSDDLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPS 300

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 301  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 360

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
             GLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 361  DGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 420

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 421  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 480

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 541  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 600

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 601  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 660

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 661  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 720

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQISS RHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 721  ETGTLEYGQVFVQISSVRHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 780

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 781  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 840

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 841  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 900

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFT 960

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 961  RDVAMRCYDSDMETEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1020

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESGPDSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1021 SFTRRRDAEAINLAARSLRKEARTWFNTKESGPDSGSDDLFAKASAWYHVTYHHSYWGSY 1080

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLRRRE+ASR+ATLDRF  VLNL GR
Sbjct: 1081 NDGMKRDHYLSFPWCVYHKLMQVKENNLRRREKASRLATLDRFSRVLNLDGR 1131

BLAST of Tan0001219 vs. ExPASy TrEMBL
Match: A0A6J1FSA2 (RNA-dependent RNA polymerase OS=Cucurbita moschata OX=3662 GN=LOC111447985 PE=3 SV=1)

HSP 1 Score: 2070.4 bits (5363), Expect = 0.0e+00
Identity = 1004/1132 (88.69%), Postives = 1063/1132 (93.90%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQF GAAQAE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFIGAAQAE 74

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLE+LTLCFGCQVS E+FHVLWEG 
Sbjct: 75   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLEDLTLCFGCQVSSEKFHVLWEGD 134

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 135  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 194

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQWVRT DFTPS S+GQSSSLCLKLPNGRQLPNFR+
Sbjct: 195  KKVAPSSGQIFDDPLFNFFKEVPDDQWVRTADFTPSNSVGQSSSLCLKLPNGRQLPNFRR 254

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+ FSCS DLVP+VDSRPNV LPYEIIFKVNVLVQNGCI GP 
Sbjct: 255  HFAYYEEFEDEFKLVDGGNSFSCSDDLVPVVDSRPNVLLPYEIIFKVNVLVQNGCIAGPS 314

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 315  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 374

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
             GLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 375  DGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 434

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 435  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 494

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 495  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 554

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 555  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 614

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 615  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 674

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 675  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 734

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQISS RHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 735  ETGTLEYGQVFVQISSVRHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 794

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 795  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 854

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 855  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 914

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 915  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKGIAPNISYIRSFT 974

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 975  RDVAMRCYDSDMETEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1034

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESGPDSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1035 SFTRRRDAEAINLAARSLRKEARTWFNTKESGPDSGSDDLFAKASAWYHVTYHHSYWGSY 1094

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLRRRE+ASR+ATLDRF  VLNL GR
Sbjct: 1095 NDGMKRDHYLSFPWCVYHKLMQVKENNLRRREKASRLATLDRFSRVLNLDGR 1145

BLAST of Tan0001219 vs. ExPASy TrEMBL
Match: A0A6J1K2P9 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV=1)

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1005/1132 (88.78%), Postives = 1064/1132 (93.99%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQFTGAAQAE
Sbjct: 1    MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLENLTLCFGCQVS E+FHVLWEG 
Sbjct: 61   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLWEGD 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 121  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQ VRT DFTPS SIGQSSSLCLKLPNGRQLPNFR+
Sbjct: 181  KKVAPSSGQIFDDPLFNFFKEVPDDQCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRR 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+GFSCS DLVP+VDS PNV LPYEIIFKVNVLVQNGCIPGP 
Sbjct: 241  HFAYYEEFEDEFKLVDGGNGFSCSDDLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPS 300

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 301  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 360

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
            AGLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 420

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 421  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 480

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDF+HIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 481  IREWMGDFQHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 541  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 600

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 601  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 660

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 661  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 720

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQIS+ARHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 721  ETGTLEYGQVFVQISNARHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 780

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 781  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 840

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 841  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 900

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDR SYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFT 960

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 961  RDVAMRCYDSDMEAEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1020

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESG DSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1021 SFTRRRDAEAINLAARSLRKEARTWFNTKESGLDSGSDDLFAKASAWYHVTYHHSYWGSY 1080

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLR+RERASR+ATLDRF  VLNL GR
Sbjct: 1081 NDGMKRDHYLSFPWCVYHKLMQVKENNLRKRERASRLATLDRFSRVLNLDGR 1131

BLAST of Tan0001219 vs. ExPASy TrEMBL
Match: A0A6J1K2N6 (RNA-dependent RNA polymerase OS=Cucurbita maxima OX=3661 GN=LOC111490498 PE=3 SV=1)

HSP 1 Score: 2067.7 bits (5356), Expect = 0.0e+00
Identity = 1005/1132 (88.78%), Postives = 1064/1132 (93.99%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+TVHI+GFPSH TAD+VK FLE  TG+GTVYAIKVRPPKRGG+RVYAIVQFTGAAQAE
Sbjct: 15   MGKTVHISGFPSHATADSVKSFLELRTGLGTVYAIKVRPPKRGGSRVYAIVQFTGAAQAE 74

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             IISLAN+RLWYGSSYLKAR ADVDIVPKPRTYVHTLENLTLCFGCQVS E+FHVLWEG 
Sbjct: 75   LIISLANQRLWYGSSYLKARAADVDIVPKPRTYVHTLENLTLCFGCQVSSEKFHVLWEGD 134

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            V+LVTFG+ MRKMNF LIHNFVEYR +LSYENIWQIQLH  QHQSMK LLIQLYGAPRIY
Sbjct: 135  VELVTFGVEMRKMNFHLIHNFVEYRFQLSYENIWQIQLHRLQHQSMKCLLIQLYGAPRIY 194

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FDDP  NFFKEVPDDQ VRT DFTPS SIGQSSSLCLKLPNGRQLPNFR+
Sbjct: 195  KKVAPSSGQIFDDPLFNFFKEVPDDQCVRTADFTPSYSIGQSSSLCLKLPNGRQLPNFRR 254

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            +FAYYEEFE+EF+LVDGG+GFSCS DLVP+VDS PNV LPYEIIFKVNVLVQNGCIPGP 
Sbjct: 255  HFAYYEEFEDEFKLVDGGNGFSCSDDLVPVVDSHPNVLLPYEIIFKVNVLVQNGCIPGPS 314

Query: 301  LDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISLD 360
            LDTGFYRLVDPSRIR+E+VE+ALEKLFHLKEC YEPSSFLTEQY+KY++HP NSPAI+LD
Sbjct: 315  LDTGFYRLVDPSRIRVEYVENALEKLFHLKECCYEPSSFLTEQYKKYAKHPSNSPAIALD 374

Query: 361  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLLP 420
            AGLVYVRRVQITPCKVYFCGPEVNVSNRVLR F +D+DNFLRV+FVDEEWDKMRS DLLP
Sbjct: 375  AGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSRDLDNFLRVSFVDEEWDKMRSTDLLP 434

Query: 421  RMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAAA 480
            RM+S+I++GKTDIY RILSVL+NGI IG K F+FLAFSSSQLR+NSLWMFASRPGLDAA 
Sbjct: 435  RMASSIENGKTDIYRRILSVLKNGIAIGDKKFKFLAFSSSQLRENSLWMFASRPGLDAAV 494

Query: 481  IREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 540
            IREWMGDF+HIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI
Sbjct: 495  IREWMGDFQHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDGI 554

Query: 541  GKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDNT 600
            GKISSDFAKRVA+KC L AFTPSAFQIRYGGYKGVVAVDP SS+KLSLR SMCKFESDNT
Sbjct: 555  GKISSDFAKRVASKCVL-AFTPSAFQIRYGGYKGVVAVDPRSSVKLSLRKSMCKFESDNT 614

Query: 601  KLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALEL 660
            KLDVLGYSKYQPCFLNRQ+ITLLSTLGVKDEVFEKKQ EA+EQLDAILTDPL+AQEALEL
Sbjct: 615  KLDVLGYSKYQPCFLNRQLITLLSTLGVKDEVFEKKQSEALEQLDAILTDPLKAQEALEL 674

Query: 661  MSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLD 720
            MSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFR SKLLELRTKSRIFIP+GRA+MGCLD
Sbjct: 675  MSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRESKLLELRTKSRIFIPNGRAMMGCLD 734

Query: 721  ETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLR 780
            ETGTLEYGQVFVQIS+ARHR++SDT AF+M GSE  LVI GNV VAKNPCLHPGDVRVL 
Sbjct: 735  ETGTLEYGQVFVQISNARHRHISDTSAFDMSGSEDHLVIVGNVTVAKNPCLHPGDVRVLN 794

Query: 781  AVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTPA 840
            AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTPA
Sbjct: 795  AVNVPLLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQIPPMDYTPA 854

Query: 841  PPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSIA 900
            PPIQLDRDV IEDVQEYFVNYMVNDSLGIIANAHTAFADKE FKARS+PC+ELAKLFSIA
Sbjct: 855  PPIQLDRDVTIEDVQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSAPCIELAKLFSIA 914

Query: 901  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSFT 960
            VDFPKTGVPAIIPSHLNVKEFPDFMEKPDR SYESKNVIGKLFRAVK IAP +SYIRSFT
Sbjct: 915  VDFPKTGVPAIIPSHLNVKEFPDFMEKPDRHSYESKNVIGKLFRAVKGIAPNISYIRSFT 974

Query: 961  RDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSK 1020
            RDVAM+CYD DME EGFEDYVEDA YHK  YDYKLGNLLDYYGIKSE+EI SG+IMRMSK
Sbjct: 975  RDVAMRCYDSDMEAEGFEDYVEDAIYHKTIYDYKLGNLLDYYGIKSESEIFSGNIMRMSK 1034

Query: 1021 SFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGCY 1080
            SFTRRRDAEAINLA RSLRKEARTWFN KESG DSGSDDLFAKASAWYHVTYHHSYWG Y
Sbjct: 1035 SFTRRRDAEAINLAARSLRKEARTWFNTKESGLDSGSDDLFAKASAWYHVTYHHSYWGSY 1094

Query: 1081 NEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            N+G KRDHYLSFPWCVY KLMQ+KENNLR+RERASR+ATLDRF  VLNL GR
Sbjct: 1095 NDGMKRDHYLSFPWCVYHKLMQVKENNLRKRERASRLATLDRFSRVLNLDGR 1145

BLAST of Tan0001219 vs. ExPASy TrEMBL
Match: A0A1S3CPY1 (RNA-dependent RNA polymerase OS=Cucumis melo OX=3656 GN=LOC103502906 PE=3 SV=1)

HSP 1 Score: 1976.8 bits (5120), Expect = 0.0e+00
Identity = 969/1133 (85.53%), Postives = 1035/1133 (91.35%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+T+HI+GFPSHVTADAVK FLEGHTG GTVYAIKVRPPKRGG R+YAIVQFT A QAE
Sbjct: 1    MGKTIHISGFPSHVTADAVKNFLEGHTGPGTVYAIKVRPPKRGGGRLYAIVQFTSATQAE 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             I+SLAN+RLWYGSSYLKAR  +VDIVPKP+TY++ L++L LCFGCQVS E+F VLWEG 
Sbjct: 61   LIVSLANQRLWYGSSYLKARSTEVDIVPKPKTYMYMLKDLLLCFGCQVSSEKFRVLWEGN 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
            VDLVTFGIGMRKMNF L +N VEYRLELSYENIWQIQLH PQ QSMKYLLIQLYGAPRIY
Sbjct: 121  VDLVTFGIGMRKMNFHLKYNSVEYRLELSYENIWQIQLHSPQCQSMKYLLIQLYGAPRIY 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            KKVAPSSGQ+FD+P LNFF EVPDDQWVRT DFT SCSIGQSSSLCLKLPNG +LP F+Q
Sbjct: 181  KKVAPSSGQIFDNPILNFFMEVPDDQWVRTTDFTSSCSIGQSSSLCLKLPNGLELPTFKQ 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGPL 300
            NFAYYEEFE+EF L+D    FS  +DL P+VDSRP+V LPYEIIFK+N LVQ+GCIP  L
Sbjct: 241  NFAYYEEFEHEFRLIDEDASFSFCRDLAPIVDSRPHV-LPYEIIFKINALVQHGCIPWSL 300

Query: 301  LDTGFYRLVDP-SRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPNSPAISL 360
            LDT FYRLV+    IRIEFVEHALEKLFHLKEC+Y+PS+FLTEQ+R+YSRHPPNSP ISL
Sbjct: 301  LDTSFYRLVERIITIRIEFVEHALEKLFHLKECNYDPSNFLTEQFRRYSRHPPNSPVISL 360

Query: 361  DAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSIDLL 420
            D GLVYVRRVQITPCKVYFCGPEVNVSNRVLR F + IDNFLRV+FVDEEWDKMRS DLL
Sbjct: 361  DDGLVYVRRVQITPCKVYFCGPEVNVSNRVLRHFSKYIDNFLRVSFVDEEWDKMRSTDLL 420

Query: 421  PRMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLDAA 480
            PRMSS  +D KTDIY RILSVL+NGIVIG K F+FLAFSSSQLRDNSLWMFAS P +DAA
Sbjct: 421  PRMSSKSEDSKTDIYRRILSVLKNGIVIGDKTFQFLAFSSSQLRDNSLWMFASGPDIDAA 480

Query: 481  AIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFSDG 540
             IR WMGDFRHIKNPAKYAARLGQSFGSSTE LSVA +EREIIPDIEVQHGEVKYVFSDG
Sbjct: 481  YIRAWMGDFRHIKNPAKYAARLGQSFGSSTEALSVASNEREIIPDIEVQHGEVKYVFSDG 540

Query: 541  IGKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFESDN 600
            IGKISS FAK VA KC  QA  PSAFQIRYGGYKGVVAVDP S+IKLSLR SMCKFESDN
Sbjct: 541  IGKISSKFAKEVATKCGFQA-VPSAFQIRYGGYKGVVAVDPYSTIKLSLRKSMCKFESDN 600

Query: 601  TKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALE 660
             KLDVLG+SKYQPCFLNRQ+ITLLSTLGV+DE+FEKKQ EAVEQLDAILTDPL+AQEALE
Sbjct: 601  IKLDVLGHSKYQPCFLNRQLITLLSTLGVRDEIFEKKQSEAVEQLDAILTDPLKAQEALE 660

Query: 661  LMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCL 720
            LMSPGENTNILKEMLKCGYKPD+EPYLSMMLQTFRASKLLELRTKSRIFIP+GRA+MGCL
Sbjct: 661  LMSPGENTNILKEMLKCGYKPDVEPYLSMMLQTFRASKLLELRTKSRIFIPNGRAMMGCL 720

Query: 721  DETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVL 780
            DET TLEYGQVFVQIS  RHRNLS++FAFN  G E CLVIEG V VAKNPCLHPGDVRVL
Sbjct: 721  DETMTLEYGQVFVQISGGRHRNLSESFAFN-SGQEHCLVIEGKVTVAKNPCLHPGDVRVL 780

Query: 781  RAVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDYTP 840
            +AVN+P LYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQ+ PMDYTP
Sbjct: 781  KAVNVPGLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQITPMDYTP 840

Query: 841  APPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLFSI 900
            A PI+LDRDV  ED+QEYFVNYMVNDSLGIIANAHTAFADKE FKARSSPC+ELAK FSI
Sbjct: 841  ALPIELDRDVTTEDIQEYFVNYMVNDSLGIIANAHTAFADKEPFKARSSPCVELAKQFSI 900

Query: 901  AVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIRSF 960
            AVDFPKTGVPAIIPSHL VKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPT+S+I+ F
Sbjct: 901  AVDFPKTGVPAIIPSHLYVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTLSHIQPF 960

Query: 961  TRDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMS 1020
            TRDVA +CYD DMEVEGFEDY EDAFYHK+NYD KLGNLLDYYGIKSEAEILSGSIMRMS
Sbjct: 961  TRDVARRCYDCDMEVEGFEDYFEDAFYHKSNYDDKLGNLLDYYGIKSEAEILSGSIMRMS 1020

Query: 1021 KSFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYWGC 1080
            KSFTRRRDAEAINLAVRSLRKEARTWFNA+E G DS SDDLFAKASAWY+VTYHHSYWGC
Sbjct: 1021 KSFTRRRDAEAINLAVRSLRKEARTWFNARE-GADSNSDDLFAKASAWYYVTYHHSYWGC 1080

Query: 1081 YNEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLGGR 1133
            YNEG KRDHYLSFPWC+YDKLMQIKENNLR+RERA+R+AT DRFG VLNLGGR
Sbjct: 1081 YNEGMKRDHYLSFPWCIYDKLMQIKENNLRKRERAARLATFDRFGHVLNLGGR 1129

BLAST of Tan0001219 vs. TAIR 10
Match: AT1G14790.1 (RNA-dependent RNA polymerase 1 )

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 721/1133 (63.64%), Postives = 859/1133 (75.82%), Query Frame = 0

Query: 1    MGRTVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGGTRVYAIVQFTGAAQAE 60
            MG+T+ + GFP+ V+A+ VK FLE  TG GTVYAIKVR PK+GG RVYAIVQFT      
Sbjct: 1    MGKTIQVFGFPNGVSAEEVKKFLERLTGSGTVYAIKVRQPKKGGPRVYAIVQFTSERHTR 60

Query: 61   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGERFHVLWEGG 120
             II+ A  RL+YG SYLKA   + DIVPKPR  +HT+  L + FGCQVS ++F  LW   
Sbjct: 61   LIITAAAERLYYGRSYLKAFEVEQDIVPKPRASLHTISGLKMFFGCQVSTKKFLTLWSAQ 120

Query: 121  VDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIY 180
               V+FGIGMRK++F       +YRLELSYENIWQI LH PQ +S K+L+IQ+ GAP+I+
Sbjct: 121  DVCVSFGIGMRKLHFSFSWYQKDYRLELSYENIWQIDLHSPQGRSSKFLVIQVIGAPKIF 180

Query: 181  KKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQLPNFRQ 240
            +K       +F    ++F+ +  D+QW+RT DFT S  IGQS++ CL+LP    +P+FR+
Sbjct: 181  EKEDQPINLLFG--IMDFYSDGSDEQWIRTTDFTSSSCIGQSTAFCLELPVHLNVPDFRE 240

Query: 241  NFAYYEEFENEFELVDGGDGFSCSKD-LVPMVDSRPNVFLPYEIIFKVNVLVQNGCIPGP 300
            NFA Y E      L++ G  +S + + LVP+VD  P   LP+EI+FK+N LVQN C+ GP
Sbjct: 241  NFANYAEHRASSFLIESGSSYSSNANTLVPVVDPPPGFSLPFEILFKLNTLVQNACLSGP 300

Query: 301  LLDTGFYRLVDPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKY--SRHPPNSPAI 360
             LD  FYRL++  +     ++H LEKLFHL EC YEP+ +L ++Y+K+      P SP I
Sbjct: 301  ALDLDFYRLLNQKKYDRALIDHCLEKLFHLGECCYEPAHWLRDEYKKWISKGKLPLSPTI 360

Query: 361  SLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEEWDKMRSID 420
            SLD GLVY+ RVQ+TP +VYF GPEVNVSNRVLR + + I+NFLRV+FVDE+ +K+RS+D
Sbjct: 361  SLDDGLVYMYRVQVTPARVYFSGPEVNVSNRVLRHYSKYINNFLRVSFVDEDLEKVRSMD 420

Query: 421  LLPRMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSLWMFASRPGLD 480
            L PR S+     +T +Y RI SVLR+GIVIG K FEFLAFSSSQLR+NS WMFA    + 
Sbjct: 421  LSPRSST---QRRTKLYDRIYSVLRDGIVIGDKKFEFLAFSSSQLRENSAWMFAPIDRIT 480

Query: 481  AAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEVQHGEVKYVFS 540
            AA IR WMGDF HI+N AKYAARLGQSF SS ETL+V   E E+IPD+E+     +YVFS
Sbjct: 481  AAHIRAWMGDFDHIRNVAKYAARLGQSFSSSRETLNVRSDEIEVIPDVEIISLGTRYVFS 540

Query: 541  DGIGKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLSLRNSMCKFES 600
            DGIGKIS++FA++VA KC L  F+PSAFQIRYGGYKGVVAVDP SS KLSLR SM KFES
Sbjct: 541  DGIGKISAEFARKVARKCGLTEFSPSAFQIRYGGYKGVVAVDPNSSKKLSLRKSMSKFES 600

Query: 601  DNTKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEA 660
            +NTKLDVL +SKYQPC++NRQ+ITLLSTLGV D VFEKKQRE V++LDAILT PL A EA
Sbjct: 601  ENTKLDVLAWSKYQPCYMNRQLITLLSTLGVTDSVFEKKQREVVDRLDAILTHPLEAHEA 660

Query: 661  LELMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMG 720
            L LM+PGENTNILK ++ CGYKPD EP+LSMMLQ FRASKLLELRTK+RIFI  GR++MG
Sbjct: 661  LGLMAPGENTNILKALILCGYKPDAEPFLSMMLQNFRASKLLELRTKTRIFISGGRSMMG 720

Query: 721  CLDETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVR 780
            CLDET TLEYGQV VQ S              MR   R  +I G V+VAKNPCLHPGDVR
Sbjct: 721  CLDETRTLEYGQVVVQYSDP------------MRPGRR-FIITGPVVVAKNPCLHPGDVR 780

Query: 781  VLRAVNIPRLYHMVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELIPPRQVLPMDY 840
            VL+AVN+P L HMVDCVVFPQKG RPHPNECSGSDLDGDIYFVCWD EL+PPR   PMDY
Sbjct: 781  VLQAVNVPALNHMVDCVVFPQKGLRPHPNECSGSDLDGDIYFVCWDQELVPPRTSEPMDY 840

Query: 841  TPAPPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKARSSPCLELAKLF 900
            TP P   LD DV IE+V+EYF NY+VNDSLGIIANAHTAFADKE  KA S PC+ELAK F
Sbjct: 841  TPEPTQILDHDVTIEEVEEYFANYIVNDSLGIIANAHTAFADKEPLKAFSDPCIELAKKF 900

Query: 901  SIAVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRAVKDIAPTVSYIR 960
            S AVDFPKTGV A+IP HL VKE+PDFMEKPD+P+YESKNVIGKLFR VK+ AP +  I+
Sbjct: 901  STAVDFPKTGVAAVIPQHLYVKEYPDFMEKPDKPTYESKNVIGKLFREVKERAPPLISIK 960

Query: 961  SFTRDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIKSEAEILSGSIMR 1020
            SFT DVA + YD DMEV+GFE+YV++AFY K NYD+KLGNL+DYYGIK+EAEILSG IMR
Sbjct: 961  SFTLDVASKSYDKDMEVDGFEEYVDEAFYQKANYDFKLGNLMDYYGIKTEAEILSGGIMR 1020

Query: 1021 MSKSFTRRRDAEAINLAVRSLRKEARTWFNAKESGPDSGSDDLFAKASAWYHVTYHHSYW 1080
            MSKSFT+RRDAE+I  AVR+LRKE  + FNA E       ++  AKASAWYHVTYH SYW
Sbjct: 1021 MSKSFTKRRDAESIGRAVRALRKETLSLFNASEE-----EENESAKASAWYHVTYHSSYW 1080

Query: 1081 GCYNEGTKRDHYLSFPWCVYDKLMQIKENNLRRRERASRMATLDRFGSVLNLG 1131
            G YNEG  RDH+LSF WCVYDKL++IK+ NL RR+   R  TL+R   VL  G
Sbjct: 1081 GLYNEGLNRDHFLSFAWCVYDKLVRIKKTNLGRRQ---RQETLERLDHVLRFG 1107

BLAST of Tan0001219 vs. TAIR 10
Match: AT4G11130.1 (RNA-dependent RNA polymerase 2 )

HSP 1 Score: 761.1 bits (1964), Expect = 1.2e-219
Identity = 450/1133 (39.72%), Postives = 661/1133 (58.34%), Query Frame = 0

Query: 4    TVHINGFPSHVTADAVKIFLEGHTGVGTVYAIKVRPPKRGG--TRVYAIVQFTG-AAQAE 63
            TV I+  P  + AD +  FLE H G  TV+A+++ P  R     R +A VQFT    ++ 
Sbjct: 11   TVKISNVPQTIVADELLRFLELHLGEDTVFALEI-PTTRDNWKPRDFARVQFTTLEVKSR 70

Query: 64   SIISLANRRLWYGSSYLKARVADVDIVPKPRTYVHTLENLTLCFGCQVSGE-RFHVL--W 123
            + +  +  +L + +  L+   A  DI+P+P      L+++ L  G   S E RF  L  W
Sbjct: 71   AQLLSSQSKLLFKTHNLRLSEAYDDIIPRPVDPRKRLDDIVLTVGFPESDEKRFCALEKW 130

Query: 124  EGGVDLVTFGIGMRKMNFCLIHNFVEYRLELSYENIWQIQ--LHHPQHQSMKYLLIQLYG 183
            +G    +      R++ F +  +   Y++E+ +E+I +      +     +   L++L  
Sbjct: 131  DGVRCWIL--TEKRRVEFWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKY 190

Query: 184  APRIYKKVAPSSGQVFDDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKLPNGRQL 243
             P+++K+V       F      F KE  D  W+RT DF+ S SIG S+  CL++ NG  +
Sbjct: 191  GPKVFKRVTVHIATKFKSDRYRFCKEDFDFMWIRTTDFSGSKSIGTSTCFCLEVHNGSTM 250

Query: 244  PNFRQNFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSR-PNVFLPYEIIFKVNVLVQNG 303
             +      YY E       VD G  F+ +  +VP++++    +  PYEI+F++N LV   
Sbjct: 251  LDIFSGLPYYREDTLSLTYVD-GKTFASAAQIVPLLNAAILGLEFPYEILFQLNALVHAQ 310

Query: 304  CIPGPLLDTGFYRLVDPSR-IRIEFVEHALEKLFHLKECSYEPSSFLTEQYRKYSRHPPN 363
             I   L       L+   R + +E     L+KL       Y+P  F+  Q +   +   +
Sbjct: 311  KI--SLFAASDMELIKILRGMSLETALVILKKLHQQSSICYDPVFFVKTQMQSVVKKMKH 370

Query: 364  SPAIS----LDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNFLRVTFVDEE 423
            SPA +     +  ++  +R  +TP K+Y  GPE+  +N V++ F + + +F+RVTFV+E+
Sbjct: 371  SPASAYKRLTEQNIMSCQRAYVTPSKIYLLGPELETANYVVKNFAEHVSDFMRVTFVEED 430

Query: 424  WDKM--RSIDLLPRMSSTIKDGKTDIYSRILSVLRNGIVIGAKNFEFLAFSSSQLRDNSL 483
            W K+   ++ +  +    +K  +T+IY+R+LS+L  GI +G K FEFLAFS+SQLR NS+
Sbjct: 431  WSKLPANALSVNSKEGYFVKPSRTNIYNRVLSILGEGITVGPKRFEFLAFSASQLRGNSV 490

Query: 484  WMFASRPGLDAAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLSVARHEREIIPDIEV 543
            WMFAS   + A  IREWMG FR I++ +K AAR+GQ F +S +TL V   + E IPDIEV
Sbjct: 491  WMFASNEKVKAEDIREWMGCFRKIRSISKCAARMGQLFSASRQTLIVRAQDVEQIPDIEV 550

Query: 544  QHGEVKYVFSDGIGKISSDFAKRVAAKCSLQAFTPSAFQIRYGGYKGVVAVDPLSSIKLS 603
                  Y FSDGIGKIS  FAK+VA KC L +  PSAFQIRYGGYKGV+AVD  S  KLS
Sbjct: 551  TTDGADYCFSDGIGKISLAFAKQVAQKCGL-SHVPSAFQIRYGGYKGVIAVDRSSFRKLS 610

Query: 604  LRNSMCKFESDNTKLDVLGYSKYQPCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAI 663
            LR+SM KF+S+N  L+V  +++  PCFLNR++I LLSTLG++D +FE  Q   +  L  +
Sbjct: 611  LRDSMLKFDSNNRMLNVTRWTESMPCFLNREIICLLSTLGIEDAMFEAMQAVHLSMLGNM 670

Query: 664  LTDPLRAQEALELMSPGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRI 723
            L D   A   L+ +S   + N+L +ML  GY P  EPYLSMML+    S+L EL+++ RI
Sbjct: 671  LEDRDAALNVLQKLSGENSKNLLVKMLLQGYAPSSEPYLSMMLRVHHESQLSELKSRCRI 730

Query: 724  FIPDGRAVMGCLDETGTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAK 783
             +P GR ++GC+DE G LEYGQV+V+++  +    S   ++  +  E   V+ G V+V K
Sbjct: 731  LVPKGRILIGCMDEMGILEYGQVYVRVTLTKAELKSRDQSYFRKIDEETSVVIGKVVVTK 790

Query: 784  NPCLHPGDVRVLRAVNIPRLYH--MVDCVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTE 843
            NPCLHPGD+RVL A+          +DC++FPQKG RPHPNECSG DLDGD +FV WD +
Sbjct: 791  NPCLHPGDIRVLDAIYEVHFEEKGYLDCIIFPQKGERPHPNECSGGDLDGDQFFVSWDEK 850

Query: 844  LIPPRQVLPMDYTPAPPIQLDRDVRIEDVQEYFVNYMVNDSLGIIANAHTAFADKESFKA 903
            +IP     PMDY  + P  +D DV +E++ ++FV+YM++D+LG+I+ AH   AD++  KA
Sbjct: 851  IIPSEMDPPMDYAGSRPRLMDHDVTLEEIHKFFVDYMISDTLGVISTAHLVHADRDPEKA 910

Query: 904  RSSPCLELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDFMEKPDRPSYESKNVIGKLFRA 963
            RS  CLELA L S AVDF KTG PA +P  L  +EFPDF+E+ ++P+Y S++V GKL+RA
Sbjct: 911  RSQKCLELANLHSRAVDFAKTGAPAEMPYALKPREFPDFLERFEKPTYISESVFGKLYRA 970

Query: 964  VKDIAPTVSYIRSFTRDVAMQCYDFDMEVEGFEDYVEDAFYHKNNYDYKLGNLLDYYGIK 1023
            VK    +++  +          YD  +E  GFE ++E A  H++ Y  KL +L+ YYG  
Sbjct: 971  VKS---SLAQRKPEAESEDTVAYDVTLEEAGFESFIETAKAHRDMYGEKLTSLMIYYGAA 1030

Query: 1024 SEAEILSGSI----MRMSKSFTRRRD-AEAINLAVRSLRKEARTWFNAKESGPDSGSDDL 1083
            +E EIL+G +    M +++   R  D  + I L+V+ L KEA  WF        S  D+ 
Sbjct: 1031 NEEEILTGILKTKEMYLARDNRRYGDMKDRITLSVKDLHKEAMGWFE------KSCEDEQ 1090

Query: 1084 FAK--ASAWYHVTYHHSYWGCYNEGTKRDHYLSFPWCVYDKLMQIKENNLRRR 1112
              K  ASAWY+VTY+       N   ++  +LSFPW V D L+ IK  N +R+
Sbjct: 1091 QKKKLASAWYYVTYNP------NHRDEKLTFLSFPWIVGDVLLDIKAENAQRQ 1121

BLAST of Tan0001219 vs. TAIR 10
Match: AT3G49500.1 (RNA-dependent RNA polymerase 6 )

HSP 1 Score: 589.0 bits (1517), Expect = 8.3e-168
Identity = 401/1102 (36.39%), Postives = 574/1102 (52.09%), Query Frame = 0

Query: 92   TYVHTLENLTLCFGCQVSGERFHVLWEG-GVDLV------TFGIGMRK-----MNFCLIH 151
            T  + L  +TL  G  VS + F V W   GVD +      T     RK         ++H
Sbjct: 127  TVPYKLAGITLEIGTLVSRDDFFVSWRAEGVDFLVDPFDNTCKFCFRKSTAFSFKDAVMH 186

Query: 152  NFV--EYRLELSYENIWQIQLHHPQHQSMKYLLIQLYGAPRIYKKVAPSS------GQVF 211
              +  +Y+LEL   +I  ++ +   H  +  L++QL  +PR++ + A         G + 
Sbjct: 187  AVINCDYKLELLVRDIQTVRQYKTLHGFV--LILQLASSPRVWYRTADDDIYDTVPGDLL 246

Query: 212  DDPHLNFFKEVPDDQWVRTDDFTPSCSIGQSSSLCLKL---------------------- 271
            DD          DD W+RT DFT   +IG+  S  + +                      
Sbjct: 247  DD----------DDPWIRTTDFTQVGAIGRCHSYRVLISPRYENKLRTALDYFRMRRVQE 306

Query: 272  PNGRQLPNFRQNFAYYEEFENEFELVDGGDGFSCSKDLVPMVDSRPNVFLPYEIIFKVNV 331
               R  P  R    + E   + F  +   +G S                  +EI+F VN 
Sbjct: 307  ERVRWPPRIRNEPCFGEPVSDHFFCIHHKEGIS------------------FEIMFLVNS 366

Query: 332  LVQNGCIPGPLLDTGFYRLV--DPSRIRIEFVEHALEKLFHLKECSYEPSSFLTEQYRK- 391
            ++  G      L   F+ L+   P  + I  ++H          C+Y+   F  + Y++ 
Sbjct: 367  VLHRGVFNQFQLTERFFDLLRNQPKDVNIASLKHL---------CTYKRPVF--DAYKRL 426

Query: 392  ------YSRHPPNSPAISLDAGLVYVRRVQITPCKVYFCGPEVNVSNRVLRQFPQDIDNF 451
                    ++P    +      +  +RR+ ITP + Y   PEV +SNRVLR++    + F
Sbjct: 427  KLVQEWIQKNPKLLGSHEQSEDISEIRRLVITPTRAYCLPPEVELSNRVLRRYKAVAERF 486

Query: 452  LRVTFVDEEWDKMRSIDLLPRMSSTIKD-------GKTDIYSRILSVLRNGIVIGAKNFE 511
            LRVTF+DE    + S  L   ++  +KD        KT ++ R+ S+L +G  +  + + 
Sbjct: 487  LRVTFMDESMQTINSNVLSYFVAPIVKDLTSSSFSQKTYVFKRVKSILTDGFKLCGRKYS 546

Query: 512  FLAFSSSQLRDNSLWMFASRPGLDAAAIREWMGDFRHIKNPAKYAARLGQSFGSSTETLS 571
            FLAFS++QLRD S W FA       + I+ WMG F+  KN AK AAR+G  F S+  T+ 
Sbjct: 547  FLAFSANQLRDRSAWFFAEDGKTRVSDIKTWMGKFKD-KNVAKCAARMGLCFSSTYATVD 606

Query: 572  VARHEREI-IPDIEVQHGEVKYVFSDGIGKISSDFAKRVAAKCSLQA-FTPSAFQIRYGG 631
            V  HE +  +PDIE ++G   YVFSDGIG I+ D A  V  K  L   ++P A+QIRY G
Sbjct: 607  VMPHEVDTEVPDIE-RNG---YVFSDGIGTITPDLADEVMEKLKLDVHYSPCAYQIRYAG 666

Query: 632  YKGVVAVDPLSS--IKLSLRNSMCKFESDNTKLDVLGYSKYQPCFLNRQVITLLSTLGVK 691
            +KGVVA  P  S  I+L+LR+SM KF S +T L++  ++++QP FLNRQ+ITLLS LGV 
Sbjct: 667  FKGVVARWPSKSDGIRLALRDSMKKFFSKHTILEICSWTRFQPGFLNRQIITLLSVLGVP 726

Query: 692  DEVFEKKQREAVEQLDAILTDPLRAQEALELMSPGENTNILKEMLKCGYKPDIEPYLSMM 751
            DE+F   Q   + +L+ IL D   A E L   S  E  N    ML  G+KP  EP+L  M
Sbjct: 727  DEIFWDMQESMLYKLNRILDDTDVAFEVL-TASCAEQGNTAAIMLSAGFKPKTEPHLRGM 786

Query: 752  LQTFRASKLLELRTKSRIFIPDGRAVMGCLDETGTLEYGQVFVQISSARHRNLSDTFAFN 811
            L + R ++L  LR KSRIF+  GR +MGCLDE G LE+GQ F+Q+S     N        
Sbjct: 787  LSSVRIAQLWGLREKSRIFVTSGRWLMGCLDEAGILEHGQCFIQVSKPSIENCFSKHGSR 846

Query: 812  MRGSERCL-VIEGNVIVAKNPCLHPGDVRVLRAVNIPRLYHMVDCVVFPQKGSRPHPNEC 871
             + ++  L V++G V +AKNPCLHPGDVR+L AV++P+L+HM DC++FPQKG RPH NE 
Sbjct: 847  FKETKTDLEVVKGYVAIAKNPCLHPGDVRILEAVDVPQLHHMYDCLIFPQKGDRPHTNEA 906

Query: 872  SGSDLDGDIYFVCWDTELIPP-RQVLP-MDYTPAPPIQLDRDVRIEDVQEYFVNYMVNDS 931
            SGSDLDGD+YFV WD +LIPP R+  P M Y  A    L R V  +D+ ++F   + N+ 
Sbjct: 907  SGSDLDGDLYFVAWDQKLIPPNRKSYPAMHYDAAEEKSLGRAVNHQDIIDFFARNLANEQ 966

Query: 932  LGIIANAHTAFADKESFKARSSPCLELAKLFSIAVDFPKTGVPAIIPSHLNVKEFPDFME 991
            LG I NAH   AD+  + A    CL LA+L + AVDFPKTG    +P HL  K +PDFM 
Sbjct: 967  LGTICNAHVVHADRSEYGAMDEECLLLAELAATAVDFPKTGKIVSMPFHLKPKLYPDFMG 1026

Query: 992  KPDRPSYESKNVIGKLFRAVKDIAPTVSYIRS-FTRDVAMQCYDFDMEVEGFEDYVEDAF 1051
            K D  +Y+S  ++G+L+R VK++    +   S  + D +   YD  +E+ GFED + +A+
Sbjct: 1027 KEDYQTYKSNKILGRLYRRVKEVYDEDAEASSEESTDPSAIPYDAVLEIPGFEDLIPEAW 1086

Query: 1052 YHKNNYDYKLGNLLDYYGIKSEAEILSGSIMRMSKSFTRRRD--AEAINLAVRSLRKEAR 1111
             HK  YD +L  LL  Y ++ E EI++G I  M K  ++++    E +  +  SL+KE R
Sbjct: 1087 GHKCLYDGQLIGLLGQYKVQKEEEIVTGHIWSMPKYTSKKQGELKERLKHSYNSLKKEFR 1146

Query: 1112 TWFNAKESGPDSGSDD-----LFAKASAWYHVTYHHSYWGCYNEGTKRDH-----YLSFP 1116
              F       ++ S++        KASAWYHVTYH  +     E    D       LSF 
Sbjct: 1147 KVFEETIPDHENLSEEEKNILYEKKASAWYHVTYHPEWVKKSLELQDPDESSHAAMLSFA 1178

BLAST of Tan0001219 vs. TAIR 10
Match: AT2G19910.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 124.4 bits (311), Expect = 5.8e-28
Identity = 147/609 (24.14%), Postives = 241/609 (39.57%), Query Frame = 0

Query: 432 DIYSRILSVLRNGIVIGAKNFEFLAFSSSQLR------------------DNSLWMFASR 491
           D+YS    + +NGI++G + + F  F                        D++  +    
Sbjct: 266 DLYSTYKGIAKNGIMVGLRRYRFFVFKDGGKEEKKKDVSTKGVKCYFIRTDSTASIDMQN 325

Query: 492 PGLDAA-AIREWMGDFRHIKNPA---KYAARLGQSFGSSTETLSV--ARHEREIIPDIEV 551
           P + A  ++ E    F H+   +    Y +R      S T+TL V       E I DI  
Sbjct: 326 PYIFAGKSMHEARMHFMHVNTLSSLPNYMSRFSLIL-SKTKTLEVDMTGITFEQIDDIHC 385

Query: 552 QHGEVKYVF---------SDGIGKISSDFAK---------------RVAAK--------- 611
              + K V          SDG G IS D A+                + +K         
Sbjct: 386 HDQDDKDVLDKNGKPCIHSDGTGYISEDLARMCPVNIFKGKSMRSNNIQSKNLNFEGQGP 445

Query: 612 CSLQAFTPSAFQIRYGGY--KGVVAVD-PLSSIKLSLRNSMCKFESDNTKLDVLGYSKYQ 671
           C  +      F+I Y GY  KG    +  L    + +R SM K   D T  ++  ++  +
Sbjct: 446 CGQEPPLLIQFRIFYNGYAVKGTFLTNKKLPPRTVQVRPSMIKVYEDRTLSNLSTFNSLE 505

Query: 672 ---------PCFLNRQVITLLSTLGVKDEVFEKKQREAVEQLDAILTDPLRAQEALELMS 731
                       L+R ++ LLS  GV ++ F    R  +E+   I      A +A     
Sbjct: 506 VVTTSNPPRKARLSRNLVALLSYGGVPNDFFLNILRNTLEESKTIFYSERAAFKAAINYG 565

Query: 732 PGENTNILKEMLKCGYKPDIEPYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLDET 791
             + T    +M+  G   D EPYL   L     ++   L+   R  I +   +MG +D T
Sbjct: 566 DDQYT---ADMILVGIPLD-EPYLKDRLSYLLKTERNALKA-GRFPIDESYYIMGTVDPT 625

Query: 792 GTLEYGQVFVQISSARHRNLSDTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLRAV 851
           G L+  ++ V + S +                    I G+V+V +NP LH GD+ VL+A 
Sbjct: 626 GELKENEICVILHSGQ--------------------ISGDVLVYRNPGLHFGDIHVLKAT 685

Query: 852 NIPRLYHMVD----CVVFPQKGSRPHPNECSGSDLDGDIYFVCWDTELI---PPRQVLPM 911
            +  L   V      V FPQKG R   +E +G D DGD+YF+  + +L+    P +    
Sbjct: 686 YVKALEDYVGNAKFAVFFPQKGPRSLGDEIAGGDFDGDMYFISRNPKLLEHFKPSEPWVS 745

Query: 912 DYTPA------PPIQLDRDVRIEDVQEYFV--NYMVNDSLGIIANAHTAFAD-------- 947
              P+       P +L  +   E++ + F+   +   D +G+ A+      D        
Sbjct: 746 SSKPSKIYCGRKPSELSEEELEEELFKMFLKARFCKRDVIGMAADCWLGIMDPFLTLGDE 805

BLAST of Tan0001219 vs. TAIR 10
Match: AT2G19930.1 (RNA-dependent RNA polymerase family protein )

HSP 1 Score: 114.4 bits (285), Expect = 6.0e-25
Identity = 121/468 (25.85%), Postives = 203/468 (43.38%), Query Frame = 0

Query: 535 VFSDGIGKISSDFAKR-----VAAKCSLQAFTPSA----------FQIRYGGY--KG--- 594
           + SDG G IS D A+         KC        A          F++ Y GY  KG   
Sbjct: 397 IHSDGTGYISEDLARMCPLNIFKGKCLRSESIQEACYQDPPLLIQFRMFYDGYAVKGTFL 456

Query: 595 --------VVAVDPLSSIKLSLRNSMCKFESDNTKLDVLGYSKYQPCFLNRQVITLLSTL 654
                    V V P S IK+S   S+  F + N    V   +  +   L++ ++ LLS  
Sbjct: 457 LNKKLCPRTVQVRP-SMIKVSKDPSLSNFSTFNALEVVTTSNPPKRTKLSKNLVALLSYG 516

Query: 655 GVKDEVFEKKQREAVEQLDAILTDPLRAQEALELMSPGENTNILKEMLKCGY---KPDIE 714
           G+ +E F       +E+  +I  +   A  A       ++ N   +M+  G    +P ++
Sbjct: 517 GIPNEFFLDILLNTLEESKSIFYNKRAALNAALNYGEMDDQN-AAQMILVGIPLDEPHLK 576

Query: 715 PYLSMMLQTFRASKLLELRTKSRIFIPDGRAVMGCLDETGTLEYGQVFVQISSARHRNLS 774
            YLS++L+T +     +L+   ++ + +   +MG +D TG L+  +V V + S +     
Sbjct: 577 NYLSILLKTEKN----DLKA-GKLPVTESYYLMGTVDPTGALKEDEVCVILESGQ----- 636

Query: 775 DTFAFNMRGSERCLVIEGNVIVAKNPCLHPGDVRVLRAVNIPRLYHMVD----CVVFPQK 834
                          I G V+V +NP LH GD+ +L+A  +  L   V      V FPQK
Sbjct: 637 ---------------ISGEVLVYRNPGLHFGDIHILKATYVKALEEYVGNSKFAVFFPQK 696

Query: 835 GSRPHPNECSGSDLDGDIYFVCWDTELI-------PPRQVLPMDYTPA--PPIQLDRDVR 894
           G R   +E +G D DGD+YF+  + EL+       P   + P   + +   P QL  +  
Sbjct: 697 GPRSLGDEIAGGDFDGDMYFISRNPELLENFKPSEPWVSLTPPSKSNSGRAPSQLSPEEL 756

Query: 895 IEDVQEYFV--NYMVNDSLGIIANAHTAFADK---------ESFKARSSPCLELAKLFSI 948
            E++ E F+   +  ++ +GI A++     D+         E         LEL  ++  
Sbjct: 757 EEELFEMFLTAGFHASNVIGIAADSWLTIMDRFLILGDDRAEEKAEMKKKMLELIDIYYD 816