Tan0001149 (gene) Snake gourd v1

Overview


Name	Tan0001149
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
Location	LG08: 24917480 .. 24918560 (+)
RNA-Seq Expression	Tan0001149
Synteny	Tan0001149

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCAACAAACATTTTTCCATCCCCATTTAATGCTTCCTCTTTCTCTTTTTCTTTCCCTTCCCTTCCATTACAAATACTTCCATAACCCTAATTTCCTACTCCCATTCCAAACATAACCCTCCATCTTCATTTCCATGGCCATGCCTTCTTTGTTTCTCATAATTCTCCTATTCTTGTCAACTCTTTTGCATGCATGTGATGCTCGCCATCATCGATTATTCGAGGGTTCGAGAATTGAACTGAGTCCAATCTCTCAAGGGTTTCAGATGAATCAACGCGAGAGAAGTTTGGACAAAAACAAAAGTGACAAGGTTTCTTCTATGGAGATCATTGTTGGGATTGGAATACTAAAGGTATATTAAATATATTTCATATCTCTCGGTTTGTGCTTTCTAAAAATTGGCTTGTTTAGCTATTAACTTATGTAACTAAATGAGAGAATTAAAAGTGTATCGATACATGATCAATATTTTTATCGACATTTTTATATATTAAAAAAGTTCGACAAGATGCCAGAGATAAAACTTTTTTTAAATATCATAAAGGTATCAAAAATTATTATAATATATAAACTATTCGATTAAGCATGGTTGTCATGAGTATAAGAACTCAATATAATGATCAAAAACTTATTCTATTTAAAAAATCAAATCATTCTAACACGATAACTAATTATGATCATTTCCATGATTTTTGGTTGAAATATTCATCAATATATATATATATCATTATTATATTTCTATTTTACATTTTTATAAACTCGAAATTATCATTTCCATCAACATCAACATTTAACTCTCGGGTCAAGACAAGCCATTAATACAGACAAAAGAAAATGCATGGAAAAAGAGATTTGGAAATAGTGATTGCCATTAATGAACCTCGGAATAATTTTAGCAGGGAATGAAGAAGCACAGAAGGTTGGTAATGGAGCTTCCAGTGAAAGACAAAGACAAAGACAAAGACAGAGACAGAGAAAAAGTGGTGAACTTTGAGAAAAATGAGGTTGGAGAAGATATGGTAGTGATGGATTATGCACAACCCCATAGGAAACCTCCTATTCACAATGAAAAACCCTAG

mRNA sequence

CTTCAACAAACATTTTTCCATCCCCATTTAATGCTTCCTCTTTCTCTTTTTCTTTCCCTTCCCTTCCATTACAAATACTTCCATAACCCTAATTTCCTACTCCCATTCCAAACATAACCCTCCATCTTCATTTCCATGGCCATGCCTTCTTTGTTTCTCATAATTCTCCTATTCTTGTCAACTCTTTTGCATGCATGTGATGCTCGCCATCATCGATTATTCGAGGGTTCGAGAATTGAACTGAGTCCAATCTCTCAAGGGTTTCAGATGAATCAACGCGAGAGAAGTTTGGACAAAAACAAAAGTGACAAGGTTTCTTCTATGGAGATCATTGTTGGGATTGGAATACTAAAGGGAATGAAGAAGCACAGAAGGTTGGTAATGGAGCTTCCAGTGAAAGACAAAGACAAAGACAAAGACAGAGACAGAGAAAAAGTGGTGAACTTTGAGAAAAATGAGGTTGGAGAAGATATGGTAGTGATGGATTATGCACAACCCCATAGGAAACCTCCTATTCACAATGAAAAACCCTAG

Coding sequence (CDS)

ATGGCCATGCCTTCTTTGTTTCTCATAATTCTCCTATTCTTGTCAACTCTTTTGCATGCATGTGATGCTCGCCATCATCGATTATTCGAGGGTTCGAGAATTGAACTGAGTCCAATCTCTCAAGGGTTTCAGATGAATCAACGCGAGAGAAGTTTGGACAAAAACAAAAGTGACAAGGTTTCTTCTATGGAGATCATTGTTGGGATTGGAATACTAAAGGGAATGAAGAAGCACAGAAGGTTGGTAATGGAGCTTCCAGTGAAAGACAAAGACAAAGACAAAGACAGAGACAGAGAAAAAGTGGTGAACTTTGAGAAAAATGAGGTTGGAGAAGATATGGTAGTGATGGATTATGCACAACCCCATAGGAAACCTCCTATTCACAATGAAAAACCCTAG

Protein sequence

MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQRERSLDKNKSDKVSSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQPHRKPPIHNEKP

Homology

[Close]

BLAST of Tan0001149 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 6.7e-05
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 108 EVGEDMVVMDYAQPHRKPPIHNEK 132
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

[Close]

BLAST of Tan0001149 vs. NCBI nr
Match: XP_023005579.1 (uncharacterized protein LOC111498521 [Cucurbita maxima])

HSP 1 Score: 167.2 bits (422), Expect = 9.8e-38
Identity = 91/132 (68.94%), Postives = 108/132 (81.82%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQRERSLDKNKSDKV 60
           M MP+   + LLFLST LHACDAR  +L   SR+E   IS GF+M+Q +RSLDK+KS++V
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60

Query: 61  SSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQ 120
           SS+E  VGI ILKGMKK RRL MELPV+D+D+DKD+D +KVVN E+NEVGEDMVVMDYAQ
Sbjct: 61  SSVE-NVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQ 120

Query: 121 PHRKPPIHNEKP 133
           PHRKPPIHN KP
Sbjct: 121 PHRKPPIHNRKP 131

[Close]

BLAST of Tan0001149 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 164.1 bits (414), Expect = 8.3e-37
Identity = 93/132 (70.45%), Postives = 106/132 (80.30%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQRERSLDKNKSDKV 60
           M MP+ F + LLFLST LHACDAR  +L   SR+E   IS GF+ NQ ERSLD++KS+ V
Sbjct: 1   MPMPTSFFLALLFLSTFLHACDARLLKLCHSSRLESCLISPGFRTNQLERSLDESKSNDV 60

Query: 61  SSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQ 120
           SSME  VGI ILKGMKK RRL MELPV+D+D+DKD D  KVVN E+NEVGEDMVVMDYAQ
Sbjct: 61  SSME-NVGIEILKGMKKLRRLAMELPVRDRDRDKDID--KVVNSEENEVGEDMVVMDYAQ 120

Query: 121 PHRKPPIHNEKP 133
           PHRKPPIHN+KP
Sbjct: 121 PHRKPPIHNQKP 129

[Close]

BLAST of Tan0001149 vs. NCBI nr
Match: KAG7028383.1 (Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.1 bits (396), Expect = 1.0e-34
Identity = 90/132 (68.18%), Postives = 104/132 (78.79%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQRERSLDKNKSDKV 60
           M MP+   + LLFLST LHACDAR  +L   SR+E   IS GF+ NQ E+SLD+++S+ V
Sbjct: 1   MPMPTSLFLSLLFLSTFLHACDARLLKLCHSSRLEPCMISPGFRTNQLEKSLDESRSNDV 60

Query: 61  SSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQ 120
           SSME  VGI ILKGMKK RRL MELPV+D D+DKD D  KVVN E+NEVGEDMVVMDYAQ
Sbjct: 61  SSME-NVGIEILKGMKKLRRLAMELPVRDIDRDKDID--KVVNSEENEVGEDMVVMDYAQ 120

Query: 121 PHRKPPIHNEKP 133
           PHRKPPIHN+KP
Sbjct: 121 PHRKPPIHNQKP 129

[Close]

BLAST of Tan0001149 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 139.8 bits (351), Expect = 1.7e-29
Identity = 85/135 (62.96%), Postives = 98/135 (72.59%), Query Frame = 0


Query: 3   MPSLFLIILLFLSTLLHACDARHHRLFEG----SRIELSPISQGFQMNQRERSLDKNKSD 62
           MP LFLI+L+F ST LHAC+AR  R FE     SRIE   ISQG   +Q ERS +++ SD
Sbjct: 1   MPYLFLILLIFSST-LHACEARCLRFFESSSFVSRIEPHSISQGIWADQPERSFEESNSD 60

Query: 63  KVSSMEIIVGIGILKGMKKH-RRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMD 122
           K+S  EIIVGI +LKGMKKH  RLVME P+K          EKV+N ++NEVGEDMVVMD
Sbjct: 61  KLSYKEIIVGIEMLKGMKKHGSRLVMEFPMK----------EKVLNSKENEVGEDMVVMD 120

Query: 123 YAQPHRKPPIHNEKP 133
           YAQPHRKPPIHNEKP
Sbjct: 121 YAQPHRKPPIHNEKP 124

[Close]

BLAST of Tan0001149 vs. NCBI nr
Match: XP_038905996.1 (uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida])

HSP 1 Score: 135.2 bits (339), Expect = 4.1e-28
Identity = 85/136 (62.50%), Postives = 98/136 (72.06%), Query Frame = 0


Query: 3   MPSLFLIILLFLSTLLHACDARHHRLFEG----SRIELSPISQGFQMNQRERSLDKNKSD 62
           MP LFLI+L+F ST LHAC+AR  R FE     SRIE   ISQG   +Q ERS +++ SD
Sbjct: 1   MPYLFLILLIFSST-LHACEARCLRFFESSSFVSRIEPHSISQGIWADQPERSFEESNSD 60

Query: 63  KVSSMEIIVGIGILK-GMKKH-RRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVM 122
           K+S  EIIVGI +LK GMKKH  RLVME P+K          EKV+N ++NEVGEDMVVM
Sbjct: 61  KLSYKEIIVGIEMLKQGMKKHGSRLVMEFPMK----------EKVLNSKENEVGEDMVVM 120

Query: 123 DYAQPHRKPPIHNEKP 133
           DYAQPHRKPPIHNEKP
Sbjct: 121 DYAQPHRKPPIHNEKP 125

[Close]

BLAST of Tan0001149 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.7e-38
Identity = 91/132 (68.94%), Postives = 108/132 (81.82%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQRERSLDKNKSDKV 60
           M MP+   + LLFLST LHACDAR  +L   SR+E   IS GF+M+Q +RSLDK+KS++V
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60

Query: 61  SSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQ 120
           SS+E  VGI ILKGMKK RRL MELPV+D+D+DKD+D +KVVN E+NEVGEDMVVMDYAQ
Sbjct: 61  SSVE-NVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQ 120

Query: 121 PHRKPPIHNEKP 133
           PHRKPPIHN KP
Sbjct: 121 PHRKPPIHNRKP 131

[Close]

BLAST of Tan0001149 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-26
Identity = 80/138 (57.97%), Postives = 98/138 (71.01%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHH-RLFEGSRIE-----LSPISQGFQMNQRERSLDK 60
           MA PSL  I+LL   + LHACDA H  +LFE S        L+PI Q F+ +Q ERSL+K
Sbjct: 1   MATPSLLFILLL---STLHACDACHRLQLFEYSNPVSRLGLLAPIPQEFRTDQPERSLNK 60

Query: 61  NKSDKVSSMEIIVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMV 120
           N   KVSS  + +GIGILKGMKKH+RL ME  +K+K        EK++++E+NEVGEDMV
Sbjct: 61  NIIHKVSS-SVEIGIGILKGMKKHKRLAMEFSMKEK--------EKLMSYEENEVGEDMV 120

Query: 121 VMDYAQPHRKPPIHNEKP 133
           VMDYAQPHRKPPIHN+KP
Sbjct: 121 VMDYAQPHRKPPIHNKKP 126

[Close]

BLAST of Tan0001149 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.9e-23
Identity = 77/141 (54.61%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 1   MAMPSLFLIILLFLSTLLHACDARHHRLFEGSRI-----ELSPISQGFQM--NQRERSLD 60
           MAMP  FLI+L F ST LHACDA   R F+ S       EL  +SQG     + +E S D
Sbjct: 1   MAMPYFFLILLFFSST-LHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFD 60

Query: 61  KNKSDKV-SSMEIIVGIGILKGMKKH-RRLVMELPVKDKDKDKDRDREKVVNFEKNEVGE 120
           KN +D +  + E I+GI +LKGMK+   RLVME P+K          EKV+N ++NEVGE
Sbjct: 61  KNNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMK----------EKVMNSKENEVGE 120

Query: 121 DMVVMDYAQPHRKPPIHNEKP 133
           DMVVMDYAQPHRKPPIHNEKP
Sbjct: 121 DMVVMDYAQPHRKPPIHNEKP 130

[Close]

BLAST of Tan0001149 vs. ExPASy TrEMBL
Match: A0A438K320 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.5e-07
Identity = 45/127 (35.43%), Postives = 70/127 (55.12%), Query Frame = 0


Query: 8   LIILLFLSTLLHACDARHHRLFEGSRIELSPISQGFQMNQR--ERSLDKNKSDKVSSMEI 67
           L++LL LS  +HAC+ARH  +      E+  +S+  +   +  + SL       + + E 
Sbjct: 4   LLLLLLLSLSMHACNARHLGVIGKENREVHLLSKVVEEADQVHKISLPPRLIPSMETAEK 63

Query: 68  IVGIGILKGMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVVMDYAQPHRKP 127
           +    IL G K+H RL++             D E+ V+ ++ ++ ED+VVMDYAQPHRKP
Sbjct: 64  LQPQQILPGWKRHSRLML--------GSAPHDVEETVDSKEGDIVEDIVVMDYAQPHRKP 122

Query: 128 PIHNEKP 133
           PIHN+ P
Sbjct: 124 PIHNKDP 122

[Close]

BLAST of Tan0001149 vs. ExPASy TrEMBL
Match: A0A2J6MHI4 (Uncharacterized protein OS=Lactuca sativa OX=4236 GN=LSAT_6X91520 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 8.2e-06
Identity = 48/137 (35.04%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 18  LHACDARHHRLFE---GSRI--------ELSPISQGFQMNQ---------RERSLDKNKS 77
           +HACD+RH  +F    G  +        E+   ++G   N+          E   ++ K 
Sbjct: 1   MHACDSRHLGVFHKEPGKELHLITSKTPEIRAGNEGRNCNETNMEDKESKHEFPSEELKM 60

Query: 78  DKVSSMEIIVGIGILK--GMKKHRRLVMELPVKDKDKDKDRDREKVVNFEKNEVGEDMVV 133
            K + +E+ V     K  G K H RL ME   KD        +EK+ N + + V ED+ V
Sbjct: 61  HKGNDIELKVSQDGTKPQGWKNHARLTMESKQKD-------SKEKINNSKDSSVTEDVGV 120

[Close]

BLAST of Tan0001149 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 48.5 bits (114), Expect = 4.7e-06
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 108 EVGEDMVVMDYAQPHRKPPIHNEK 132
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:

BLAST of Tan0001149 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK8	6.7e-05	83.33	Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1	[more]

BLAST of Tan0001149 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023005579.1	9.8e-38	68.94	uncharacterized protein LOC111498521 [Cucurbita maxima]	[more]
XP_023540680.1	8.3e-37	70.45	uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo]	[more]
KAG7028383.1	1.0e-34	68.18	Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_038905997.1	1.7e-29	62.96	uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida]	[more]
XP_038905996.1	4.1e-28	62.50	uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida]	[more]

BLAST of Tan0001149 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KZM9	4.7e-38	68.94	uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521...	[more]
A0A6J1DKL1	1.1e-26	57.97	uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A0A0L298	1.9e-23	54.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1	[more]
A0A438K320	2.5e-07	35.43	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1	[more]
A0A2J6MHI4	8.2e-06	35.04	Uncharacterized protein OS=Lactuca sativa OX=4236 GN=LSAT_6X91520 PE=4 SV=1	[more]

BLAST of Tan0001149 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16515.1	4.7e-06	83.33	Encodes a root meristem growth factor (RGF). Belongs to a family of functionall...	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34961:SF7	SUBFAMILY NOT NAMED	coord: 3..132
None	No IPR available	PANTHER	PTHR34961	TRANSMEMBRANE PROTEIN	coord: 3..132
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 53..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0001149.1	Tan0001149.1	mRNA