Tan0000427 (gene) Snake gourd v1

Overview
NameTan0000427
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description1-acylglycerol-3-phosphate O-acyltransferase
LocationLG02: 8966396 .. 8970140 (-)
RNA-Seq ExpressionTan0000427
SyntenyTan0000427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTTCTCCAGACACTGTTATCCGTCACAACGTCGAAGCACCACGAAGGGAAGGCAGTGGCAAGATCGTAGTTTCAAACTCTCCATTGTCCTTTTTTGTAAATTCACAAAACCAACCTGACACAACCAACGCTCCAGAGAATTCAGCGAGTACCGGAGCACCAATAAATCATCTCCTCCATCTCCAAGAAAAGAATCTAACTGTGTCAGTCCAGAAATTTCTTCGCCTCGTTCGTTTCTTCACTTCACCTCGCTATGAAGATCACCCTCAAATCCTGAGGCTGTCAATGCATTTGAGGTAAGCCACTTTCTTATTGCCATTTCGTCTTTTTTCTCTTTCCATATTGACAGTTTCTGTTTGGTTCCTGAGAAAATGCCGCGAATACTGTAGTAAATCTAGTCCTCTTTGTCTCACTTCTCACTGTGCTGAACCTACCGGAGAACATGCATAAATGTTCGACTGTTTTCCGCCGATTACTTCGGTGTTGTGATGTGATCTGATACGTGGTGATTTGATTGCTCGACTTCTCCCGCGGATTTTTGTGCGAGTGAACAAGATTTGGAATAGTTATTTCTTTGGCCGAATCTGATTTATATCCATTGCTTGATATTTGTTTTGTAGTATATTTATTAAGCTTTCGTTCTGTGATTTTCAAGATCATGCACATTGTGGTGTGAATGTATGATTTGAGTATATTCGGATTTCTGGATTCGCGTTAAGCTGTGTGATCTATTGTGTAGGCTGTGGACTTGTGATCGATTAAGGAACAAAATATGAAGTGCATCTCTTAGTGACCAATAGCAGGTGAGAATATATTTGCTTGTGCCATTGCTCGTTCGTCTATACCAAATTATACGAAACCTATGTAATTGTAGTATTCTATTGTATTCATTGATTTTGGTCTTTTCCGATAGGAATGTTGCAATAGCTACATGGATGAGTGTCTGCTTTAAATCGTGTAATTTTCTCTCTCACTGGGAACAATTGTTGCTCCTCTTCGAGACACAATGTGCAAGTCAAGTACCAAAAGTAATTAGATAGAGGGAAAATGTGGAATGTTTTGGATTTCTTTCTTTTCTGGGACATTGAACTCCATTCTCCTAACCAGTAACTATGTATATAGCAAGTTTCTTCAGTTTTTGAAGCTTGAAAGGAAATGCTGTGTGTTCTATCCTCCTTCCTATGGGAGGATTTTTTTTTGTCTCTGACAAAAGTGAATTGAGAGGCATATTATATGGAAAAGGGAGTAATTGCCTTTGAGGTTGGTGGGATCAACAATGGCACTTCATGCTGCATCACTAGGCACTGGCTCTTGACTGATGCGGCATTTATTACTTTCTTGCTCCCTTCTTTCTTTCCGTAAAGTTCTGTATGTTTTCAATTCTTGCTCTCGTGTAATCTTTTAGATGAACTTTCCATTCTTATTTAATTGTTTAGTTTCATAACAGAGCATTTTAGAGGAATTCATCTGCAGCTAAATCGTTCTGTGTTAGAACCATATCACTTTCACGAGCCACAACTGCATTCCATTGGACACTACCTTGCTGAAAAGCAAGAAGTTCATCTTATACTATTAATTTAGTCGATGAGATATACCTTCATGTCCTAATCACTCTATGTTGCCTTCTTAACCAGGAAACCCACTAATCTCTGGTGATAGTGGATTGGATATACGTTTGTGCTGGATATTGTGAATGATTCTGAGAGGGTATGGAAGTCAAGGATGTTGAAATGAGGGATCACACCTTAACTCCAGTTAGGCTGTTCAGGGGTCTATTGTGCTTGCTGGTGCTTCTCTTAACAGCATTTATGATGTTAGTATACTACGGTTTTATCAGTGCCATTTTTGTTCGAGTATTCAGCATACATTATAGCCGAAAATCCACTTCCTTCTTCTTTGGTTCTTGGTTAGCACTGTGGCCTTTTCTATTTGAGAAAATTAACAAAACGAAAGTTATTTTTTCTGGAGATATGGTCCCAGCAAGGGAACGTGTCTTGCTTATTGCAAACCATAGAACTGAAGTTGATTGGATGTACCTGTGGGATCTTGCAATACGAAAGGGACAACTAGGGTACATAAAGTATATACTTAAGAGCAGTTTGATGAAGCTACCAGTATTTGGTTGGGGATTTCACATATTAGAGTTCATCTCTGTGGAAAGGAAATGGGAGGCTGATGAATCAACAATGCGCCAAATGCTTTCAACTTTCAAGGATTATCATGATCCCTTATGGCTTGCCCTTTTCCCCGAAGGCACAGATTTCACGTAATTACTCGTATATCCCAGTTCCATTTCTCTTCCTTTCTTGGAAAGATAGCACTATTGTTTACATACTTGAGATAATCAACAATTCCCAGAAAAATTCTCTTGAACATTGATTCAGTTACAATTTCGATATGTAATGTTATTTTGTGCTTAGCATGTTCCATCATTCAAGCCGTACTTTTCTTGTTCAATCTGATAGAGACTGTCTAGTATAATAAGCTTCATGTCACTCTTTTCTGTTTTGTGTAGTGAACAGAAGTGCATTCGAAGTCAAAAGTATGCAGCCGAAAATGGTTTGCCCATTTTAAGAAATGTTTTGCTTCCCAAAACAAAGGGCTTCCATGCGTGCGTACAGGATTTGAGGAAATGTCTGGATGCAGGTAGTCTCCATCTTCTGTGTTTTGCTGCTTAAAATGTTTCTTCTATCTCCTTATCTAGTTTAGAATATTGAAGTGGTTGAGCTAAACATGGTGGCTAAAATTTAGATACCTCAAAGGAACCGATTTATTGTTATGGTCCTTGGATTGCGTCACCAGTTTCAAAAATGGAAACGAAAGAGATGTTAGGTTGTAACCATTTTTAGGGAAGAAATGGACAACTTGTTCAAACCATATTTGACAAGTACAAATCTGAAGATGTAAAGATGCCTTGAATCTGTTATCCGTAACTTTGGACTTATTGGACTTATTTTGTATTAGGGTTTAGGAGGGCACCATATATAAATCCTCTAAACTCCCATCTGAAAAACTCTTTGTAATTTCGTATCCTGTTTTCTCTTGAAGGTAATGAAATACTCTCTTCTTCGGCCGGTGGATGTAGCTAACACACCGTTAGTAAACTACGTAAATCTGTGTGTTGATTTTCTTCTTGCTTTGTTTACTGCTTTCCGTTTTAAAAAAAGAGTTTCATTTTTTTTCCGTGTAGTTTACGATGTGACCATCGGTTACCAACATCGGTGCCCCTCTTTATTGGACAATGTCTTTGGATTGGAACCTTCTGAAGTTCATATACACATCCAACGCATTCCCCTCGACAACATACCAACCACAGAAAATGAAGTTGCCAATTGGCTAATGGATACATTCAGCCTCAAGGACCAATTACTTGACAAATTTCATTCTCAAGGCCATTTTCCCCATGAAGGAACTGAAGGGGATCTCTCAACCATGAAATGCCTTGTTACCATCATCACTGTAATGGTCCTAACCTCCTTATCTACATACTTGACTCTTTTTTCCTCCATCTGGTTCAAAATCTATGTATCATTGGCCTGTTCCTTTCTGGCATTCGCTACCCGTTTCAACATTCGACCAATGCCAATTTTCGGCCATAAAAAATCGTCTTGAAACTTGACAGAAGGAGAGGATGTGTAGTGTTTGAAAATGTATTACAAAACTATATAGACCTACCATTAAAATATGTAATATCAAAAGATGTCATCTTCAATTTTGCGAGTTTGTAGAACATCAGTATCAGTG

mRNA sequence

TCCTTCTCCAGACACTGTTATCCGTCACAACGTCGAAGCACCACGAAGGGAAGGCAGTGGCAAGATCGTAGTTTCAAACTCTCCATTGTCCTTTTTTGTAAATTCACAAAACCAACCTGACACAACCAACGCTCCAGAGAATTCAGCGAGTACCGGAGCACCAATAAATCATCTCCTCCATCTCCAAGAAAAGAATCTAACTGTGTCAGTCCAGAAATTTCTTCGCCTCGTTCGTTTCTTCACTTCACCTCGCTATGAAGATCACCCTCAAATCCTGAGGCTGTCAATGCATTTGAGGCTGTGGACTTGTGATCGATTAAGGAACAAAATATGAAGTGCATCTCTTAGTGACCAATAGCAGGAAACCCACTAATCTCTGGTGATAGTGGATTGGATATACGTTTGTGCTGGATATTGTGAATGATTCTGAGAGGGTATGGAAGTCAAGGATGTTGAAATGAGGGATCACACCTTAACTCCAGTTAGGCTGTTCAGGGGTCTATTGTGCTTGCTGGTGCTTCTCTTAACAGCATTTATGATGTTAGTATACTACGGTTTTATCAGTGCCATTTTTGTTCGAGTATTCAGCATACATTATAGCCGAAAATCCACTTCCTTCTTCTTTGGTTCTTGGTTAGCACTGTGGCCTTTTCTATTTGAGAAAATTAACAAAACGAAAGTTATTTTTTCTGGAGATATGGTCCCAGCAAGGGAACGTGTCTTGCTTATTGCAAACCATAGAACTGAAGTTGATTGGATGTACCTGTGGGATCTTGCAATACGAAAGGGACAACTAGGGTACATAAAGTATATACTTAAGAGCAGTTTGATGAAGCTACCAGTATTTGGTTGGGGATTTCACATATTAGAGTTCATCTCTGTGGAAAGGAAATGGGAGGCTGATGAATCAACAATGCGCCAAATGCTTTCAACTTTCAAGGATTATCATGATCCCTTATGGCTTGCCCTTTTCCCCGAAGGCACAGATTTCACTGAACAGAAGTGCATTCGAAGTCAAAAGTATGCAGCCGAAAATGGTTTGCCCATTTTAAGAAATGTTTTGCTTCCCAAAACAAAGGGCTTCCATGCGTGCGTACAGGATTTGAGGAAATGTCTGGATGCAGTTTACGATGTGACCATCGGTTACCAACATCGGTGCCCCTCTTTATTGGACAATGTCTTTGGATTGGAACCTTCTGAAGTTCATATACACATCCAACGCATTCCCCTCGACAACATACCAACCACAGAAAATGAAGTTGCCAATTGGCTAATGGATACATTCAGCCTCAAGGACCAATTACTTGACAAATTTCATTCTCAAGGCCATTTTCCCCATGAAGGAACTGAAGGGGATCTCTCAACCATGAAATGCCTTGTTACCATCATCACTGTAATGGTCCTAACCTCCTTATCTACATACTTGACTCTTTTTTCCTCCATCTGGTTCAAAATCTATGTATCATTGGCCTGTTCCTTTCTGGCATTCGCTACCCGTTTCAACATTCGACCAATGCCAATTTTCGGCCATAAAAAATCGTCTTGAAACTTGACAGAAGGAGAGGATGTGTAGTGTTTGAAAATGTATTACAAAACTATATAGACCTACCATTAAAATATGTAATATCAAAAGATGTCATCTTCAATTTTGCGAGTTTGTAGAACATCAGTATCAGTG

Coding sequence (CDS)

ATGGAAGTCAAGGATGTTGAAATGAGGGATCACACCTTAACTCCAGTTAGGCTGTTCAGGGGTCTATTGTGCTTGCTGGTGCTTCTCTTAACAGCATTTATGATGTTAGTATACTACGGTTTTATCAGTGCCATTTTTGTTCGAGTATTCAGCATACATTATAGCCGAAAATCCACTTCCTTCTTCTTTGGTTCTTGGTTAGCACTGTGGCCTTTTCTATTTGAGAAAATTAACAAAACGAAAGTTATTTTTTCTGGAGATATGGTCCCAGCAAGGGAACGTGTCTTGCTTATTGCAAACCATAGAACTGAAGTTGATTGGATGTACCTGTGGGATCTTGCAATACGAAAGGGACAACTAGGGTACATAAAGTATATACTTAAGAGCAGTTTGATGAAGCTACCAGTATTTGGTTGGGGATTTCACATATTAGAGTTCATCTCTGTGGAAAGGAAATGGGAGGCTGATGAATCAACAATGCGCCAAATGCTTTCAACTTTCAAGGATTATCATGATCCCTTATGGCTTGCCCTTTTCCCCGAAGGCACAGATTTCACTGAACAGAAGTGCATTCGAAGTCAAAAGTATGCAGCCGAAAATGGTTTGCCCATTTTAAGAAATGTTTTGCTTCCCAAAACAAAGGGCTTCCATGCGTGCGTACAGGATTTGAGGAAATGTCTGGATGCAGTTTACGATGTGACCATCGGTTACCAACATCGGTGCCCCTCTTTATTGGACAATGTCTTTGGATTGGAACCTTCTGAAGTTCATATACACATCCAACGCATTCCCCTCGACAACATACCAACCACAGAAAATGAAGTTGCCAATTGGCTAATGGATACATTCAGCCTCAAGGACCAATTACTTGACAAATTTCATTCTCAAGGCCATTTTCCCCATGAAGGAACTGAAGGGGATCTCTCAACCATGAAATGCCTTGTTACCATCATCACTGTAATGGTCCTAACCTCCTTATCTACATACTTGACTCTTTTTTCCTCCATCTGGTTCAAAATCTATGTATCATTGGCCTGTTCCTTTCTGGCATTCGCTACCCGTTTCAACATTCGACCAATGCCAATTTTCGGCCATAAAAAATCGTCTTGA

Protein sequence

MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTSFFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQLGYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHRCPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFPHEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPMPIFGHKKSS
Homology
BLAST of Tan0000427 vs. ExPASy Swiss-Prot
Match: Q9LHN4 (Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=LPAT5 PE=2 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.3e-143
Identity = 245/370 (66.22%), Postives = 302/370 (81.62%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           ME K V   D  L+ +R+ RG++CL+VL+ TAFMML+++GF+SA+ +R+FSI YSRK  S
Sbjct: 1   MEKKSVPNSD-KLSLIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANHRTEVDWMY WDLA+RKGQ+
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANHRTEVDWMYFWDLALRKGQI 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +RQ++S+FKD  D LWLALFP
Sbjct: 121 GNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLRQIVSSFKDPRDALWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q+L   LDAVYDVTIGY+ R
Sbjct: 181 EGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQELSCSLDAVYDVTIGYKTR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+TF LKDQLL+ F+S GHFP
Sbjct: 241 CPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMNTFQLKDQLLNDFYSNGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKIYVSLACSFLAFATRFNIRP 360
           +EGTE + +T K L+  + V+  T++ T+LT FSS IWF+IYVSLAC +L  AT FN+R 
Sbjct: 301 NEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRIYVSLACVYLTSATHFNLRS 360

Query: 361 MPIFGHKKSS 370
           +P+    K+S
Sbjct: 361 VPLVETAKNS 369

BLAST of Tan0000427 vs. ExPASy Swiss-Prot
Match: Q8L4Y2 (Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=LPAT4 PE=2 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 7.3e-126
Identity = 215/360 (59.72%), Postives = 276/360 (76.67%), Query Frame = 0

Query: 2   EVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTSF 61
           ++K   +++  LTP+R+ RGL+ LLV L TAFM L+Y+  I+A+ +R+ S+  SRK  S 
Sbjct: 6   DLKSDNLKNRPLTPLRILRGLMILLVFLSTAFMFLLYFAPIAALGLRLLSVQQSRKVVSL 65

Query: 62  FFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQLG 121
            FG WLALWP+LFE +N T V+FSGD++P  +RVLLIANHRTEVDWMYLW++A+RKG LG
Sbjct: 66  IFGLWLALWPYLFETVNGTTVVFSGDIIPVEKRVLLIANHRTEVDWMYLWNIALRKGCLG 125

Query: 122 YIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPE 181
           YIKY+LKSSLMKLP+FGWGFH+LEFI VERK E DE  + QMLS+FKD  +PLWLALFPE
Sbjct: 126 YIKYVLKSSLMKLPIFGWGFHVLEFIPVERKREVDEPVLLQMLSSFKDPQEPLWLALFPE 185

Query: 182 GTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHRC 241
           GTDFTE+KC RSQK+AAE GLP L NVLLPKT+GF  C++ L   LDAVYD+TI Y+ RC
Sbjct: 186 GTDFTEEKCKRSQKFAAEVGLPALSNVLLPKTRGFGVCLEVLHNSLDAVYDLTIAYKPRC 245

Query: 242 PSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFPH 301
           PS +DNVFG +PSEVHIH++R+ L  IP  E E + WLMD+F LKD+LL  F++QG FP+
Sbjct: 246 PSFMDNVFGTDPSEVHIHVRRVLLKEIPANEAESSAWLMDSFKLKDKLLSDFNAQGKFPN 305

Query: 302 EGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPMP 361
           +  E +LS +KC+ T   V+ LT +  YLTL+S   FK+Y  L+ ++L FAT +  +P P
Sbjct: 306 QRPEEELSVLKCIATFAGVISLTVVFIYLTLYSHSCFKVYACLSGTYLTFATYYKFQPSP 365

BLAST of Tan0000427 vs. ExPASy Swiss-Prot
Match: Q5F3X0 (Lysocardiolipin acyltransferase 1 OS=Gallus gallus OX=9031 GN=LCLAT1 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-46
Identity = 110/320 (34.38%), Postives = 171/320 (53.44%), Query Frame = 0

Query: 19  FRGLLCLLVLLLTAFMMLVYY--GFISAIFVRVFSIHYSRKSTSFFFGSWLALWPFLFEK 78
           ++G+  ++ L L +F   ++    F+  +F+   S  + R  T     +WL L   L E 
Sbjct: 4   WKGIYFVVALFLGSFFGSIFMLGPFLPLMFI---SPAWYRWITDRIVATWLTLPVALLEM 63

Query: 79  INKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQLGYIKYILKSSLMKLPV 138
           +   KV+ +GD     ER ++I NHRT +DWM+LW+  +R   L   K  LKSSL  +P 
Sbjct: 64  VFGAKVVVTGDGFIPGERSVIIMNHRTRMDWMFLWNCLLRYSYLRLEKICLKSSLKSIPG 123

Query: 139 FGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEGTDFTEQKCIRSQKY 198
           FGW   +  FI ++RKWE D+S    ML  F D H+PL L +FPEGTD T     RS  +
Sbjct: 124 FGWAMQVAAFIFIQRKWEDDKSHFENMLHYFCDIHEPLQLLIFPEGTDLTANTKARSNDF 183

Query: 199 AAENGLPILRNVLLPKTKGFHACVQDLRK--CLDAVYDVTIGYQHRCPSLLDNVF-GLEP 258
           A +NGL     VL P+T GF   V+ LR+   LDA++D+T+ Y    P    ++  G  P
Sbjct: 184 AEKNGLRKYEYVLHPRTTGFTFVVECLREGNNLDAIHDITVAYPQNIPQTEKHLLNGNFP 243

Query: 259 SEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFPHEGTEGDLSTMKC 318
            E+H H+QR P++ +PT++ E+  W    +  K++ L +F+  G    E  +  +   K 
Sbjct: 244 KEIHFHVQRYPIETVPTSKEELQLWCQKRWEEKEERLRRFYEGGKCFDETGQSIIPPCKS 303

Query: 319 LVTIITVMVLTSLSTYLTLF 334
            + ++ V  ++ L  Y T+F
Sbjct: 304 ELRVLAVKCISLL--YWTVF 318

BLAST of Tan0000427 vs. ExPASy Swiss-Prot
Match: Q9XFW4 (1-acyl-sn-glycerol-3-phosphate acyltransferase 2 OS=Brassica napus OX=3708 GN=LPAT2 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-46
Identity = 97/257 (37.74%), Postives = 145/257 (56.42%), Query Frame = 0

Query: 92  RERVLLIANHRTEVDWMYLWDLAIRKGQLGYIKYILKSSLMKLPVFGWGFHILEFISVER 151
           +E  L++ NHR+++DW+  W LA R G LG    ++K S   LPV GW     E++ +ER
Sbjct: 82  KEHALVVCNHRSDIDWLVGWILAQRSGCLGSALAVMKKSSKFLPVIGWSMWFSEYLFLER 141

Query: 152 KWEADESTMRQMLSTFKDYHDPLWLALFPEGTDFTEQKCIRSQKYAAENGLPILRNVLLP 211
            W  DEST++  L    D+  P WLALF EGT FTE K   +Q+YAA + LP+ RNVL+P
Sbjct: 142 NWAKDESTLQSGLQRLNDFPRPFWLALFVEGTRFTEAKLKAAQEYAASSELPVPRNVLIP 201

Query: 212 KTKGFHACVQDLRKCLDAVYDVTIGYQHRC-PSLLDNVFGLEPSEVHIHIQRIPLDNIPT 271
           +TKGF + V ++R  + A+YD+T+       P  +  +F  +PS VH+HI+   + ++P 
Sbjct: 202 RTKGFVSAVSNMRSFVPAIYDMTVAIPKTSPPPTMLRLFKGQPSVVHVHIKCHSMKDLPE 261

Query: 272 TENEVANWLMDTFSLKDQLLDKFHSQGHFPHEGTEGDLSTMKCLVTIITVMVLTSLSTYL 331
            E+E+A W  D F  KD LLDK  +   FP +  +     +K L  +++   L +L    
Sbjct: 262 PEDEIAQWCRDQFVAKDALLDKHIAADTFPGQKEQNIGRPIKSLAVVVSWACLLTLGAMK 321

Query: 332 TLFSSIWFKIYVSLACS 348
            L  S  F  +  +A S
Sbjct: 322 FLHWSNLFSSWKGIALS 338

BLAST of Tan0000427 vs. ExPASy Swiss-Prot
Match: Q6IWY1 (1-acyl-sn-glycerol-3-phosphate acyltransferase 2 OS=Brassica oleracea OX=3712 GN=LPAT2 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-46
Identity = 98/259 (37.84%), Postives = 146/259 (56.37%), Query Frame = 0

Query: 92  RERVLLIANHRTEVDWMYLWDLAIRKGQLGYIKYILKSSLMKLPVFGWGFHILEFISVER 151
           +E  L++ NHR+++DW+  W LA R G LG    ++K S   LPV GW     E++ +ER
Sbjct: 83  KEHALVVCNHRSDIDWLVGWILAQRSGCLGSALAVMKKSSKFLPVIGWSMWFSEYLFLER 142

Query: 152 KWEADESTMRQMLSTFKDYHDPLWLALFPEGTDFTEQKCIRSQKYAAENGLPILRNVLLP 211
            W  DEST++  L    D+  P WLALF EGT FTE K   +Q+YAA + LP+ RNVL+P
Sbjct: 143 NWAKDESTLKSGLQRLNDFPRPFWLALFVEGTRFTEAKLKAAQEYAATSQLPVPRNVLIP 202

Query: 212 KTKGFHACVQDLRKCLDAVYDVTIGYQHRC-PSLLDNVFGLEPSEVHIHIQRIPLDNIPT 271
           +TKGF + V ++R  + A+YD+T+       P  +  +F  +PS VH+HI+   + ++P 
Sbjct: 203 RTKGFVSAVSNMRSFVPAIYDMTVAIPKTSPPPTMLRLFKGQPSVVHVHIKCHSMKDLPE 262

Query: 272 TENEVANWLMDTFSLKDQLLDKFHSQGHFPHEGTEGDLSTMKCLVTIITVMVLTSLSTYL 331
           +E+E+A W  D F  KD LLDK  +   FP +  +     +K L  +++   L +L    
Sbjct: 263 SEDEIAQWCRDQFVAKDALLDKHIAADTFPGQKEQNIDRPIKSLAVVVSWACLLTLGAMK 322

Query: 332 TLFSSIWFKIYVSLACSFL 350
            L  S  F     +A S L
Sbjct: 323 FLHWSNLFSSLKGIALSAL 341

BLAST of Tan0000427 vs. NCBI nr
Match: XP_023530104.1 (probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 713.0 bits (1839), Expect = 1.3e-201
Identity = 346/368 (94.02%), Postives = 357/368 (97.01%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVEMRD TLTP RLFRGLLCLLVLLLTAFMMLV+YGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVEMRDRTLTPARLFRGLLCLLVLLLTAFMMLVFYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLA+WPFLFEKINKTKVIFSG+MVPARERVL+IANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLAVWPFLFEKINKTKVIFSGEMVPARERVLIIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGWGFHILEFISVERKWE DESTMRQMLSTFKDYHDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEVDESTMRQMLSTFKDYHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQKCIRSQKYAAENGLPIL+N+LLPKTKGFH CVQDLRKCLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKCIRSQKYAAENGLPILKNLLLPKTKGFHTCVQDLRKCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFS KDQLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSNKDQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HEGTEGDLSTMKCLV+I T+MVLTSLSTYLT FSSIWFKIYV  ACS+LAFATRFNIRPM
Sbjct: 301 HEGTEGDLSTMKCLVSITTLMVLTSLSTYLTFFSSIWFKIYVLFACSYLAFATRFNIRPM 360

Query: 361 PIFGHKKS 369
           PIF  KKS
Sbjct: 361 PIFHRKKS 368

BLAST of Tan0000427 vs. NCBI nr
Match: KAG7022423.1 (putative 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 706.8 bits (1823), Expect = 9.6e-200
Identity = 344/369 (93.22%), Postives = 355/369 (96.21%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVEMRD TLTP RLFRGLLCLLVLLLTAFMMLV+YGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVEMRDRTLTPARLFRGLLCLLVLLLTAFMMLVFYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLA+WPFLFEKINKTKVIFSG+MVPARERVL+IANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLAVWPFLFEKINKTKVIFSGEMVPARERVLIIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGWGFHILEFISVERKWE DESTMRQMLSTFKD HDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEVDESTMRQMLSTFKDDHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQKCIRSQKYAAENGLPIL+N+LLPKTKGFH CVQDLRKCLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKCIRSQKYAAENGLPILKNLLLPKTKGFHTCVQDLRKCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFS KDQLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSSKDQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HEGTEGDLSTM CLV+I T+MVLTSLSTYLT FSSIWFKIYV  ACS+LAFATRFNIRP 
Sbjct: 301 HEGTEGDLSTMNCLVSITTLMVLTSLSTYLTFFSSIWFKIYVLFACSYLAFATRFNIRPT 360

Query: 361 PIFGHKKSS 370
           PIF  KKSS
Sbjct: 361 PIFRRKKSS 369

BLAST of Tan0000427 vs. NCBI nr
Match: XP_022931644.1 (probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931645.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931646.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931648.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931649.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931650.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931651.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931652.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931653.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931654.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931655.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931656.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931657.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931659.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931660.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931661.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931662.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata] >XP_022931663.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita moschata])

HSP 1 Score: 700.7 bits (1807), Expect = 6.9e-198
Identity = 341/367 (92.92%), Postives = 354/367 (96.46%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVEMRD TLTP RLFRGLLCLLVLLLTAFMMLV+YGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVEMRDRTLTPARLFRGLLCLLVLLLTAFMMLVFYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLA+WPFLFEKINKTKVIFSG+MVPARERVL+IANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLAVWPFLFEKINKTKVIFSGEMVPARERVLIIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGWGFHILEFISVERKWE DESTMRQMLSTFKD HDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEVDESTMRQMLSTFKDDHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQK IRSQKYAAENGLPIL+N+LLPKTKGFH CVQDLR+CLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKRIRSQKYAAENGLPILKNLLLPKTKGFHTCVQDLRQCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIP+DNIPTTENEVANWLMDTFS KDQLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPIDNIPTTENEVANWLMDTFSNKDQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HEGTEGDLSTMKCLV+I T+MVLTSLSTYLT FSSIWFKIYV  ACS+LAFATRFNIRPM
Sbjct: 301 HEGTEGDLSTMKCLVSITTLMVLTSLSTYLTFFSSIWFKIYVLFACSYLAFATRFNIRPM 360

Query: 361 PIFGHKK 368
           PIF  KK
Sbjct: 361 PIFRRKK 367

BLAST of Tan0000427 vs. NCBI nr
Match: XP_022136204.1 (probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Momordica charantia])

HSP 1 Score: 696.0 bits (1795), Expect = 1.7e-196
Identity = 335/367 (91.28%), Postives = 354/367 (96.46%), Query Frame = 0

Query: 3   VKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTSFF 62
           VKDVE R H LTP RL RGLLCLLVL+LTAFMML+YYGFISAI VRVFSIHYSRK+TSFF
Sbjct: 8   VKDVETRHHALTPARLLRGLLCLLVLILTAFMMLIYYGFISAIIVRVFSIHYSRKATSFF 67

Query: 63  FGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQLGY 122
           FGSWLALWPFLFEKINKTKVIFSG+MVPA+ERVLLIANHRTEVDWMYLWDLA+RKGQLGY
Sbjct: 68  FGSWLALWPFLFEKINKTKVIFSGEMVPAKERVLLIANHRTEVDWMYLWDLAMRKGQLGY 127

Query: 123 IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG 182
           IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG
Sbjct: 128 IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG 187

Query: 183 TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHRCP 242
           TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGY+HRCP
Sbjct: 188 TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYKHRCP 247

Query: 243 SLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFPHE 302
           SL+DNVFGLEPSEVHIHIQRIPL+NIPTTENEV NWLMD+FS+KDQLL+ F SQGHFP+E
Sbjct: 248 SLMDNVFGLEPSEVHIHIQRIPLNNIPTTENEVTNWLMDSFSVKDQLLENFSSQGHFPNE 307

Query: 303 GTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPMPI 362
           GTEGDLST+KC V+IITVMVL ++STYLT FSSIWFKIYVSLACS+LAFATRFNIRPMPI
Sbjct: 308 GTEGDLSTIKCFVSIITVMVLITISTYLTFFSSIWFKIYVSLACSYLAFATRFNIRPMPI 367

Query: 363 FGHKKSS 370
           F H+KSS
Sbjct: 368 FSHRKSS 374

BLAST of Tan0000427 vs. NCBI nr
Match: XP_038887315.1 (probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida] >XP_038887316.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida] >XP_038887317.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida] >XP_038887318.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida] >XP_038887319.1 probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida])

HSP 1 Score: 694.9 bits (1792), Expect = 3.8e-196
Identity = 336/369 (91.06%), Postives = 352/369 (95.39%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVE RDHTLTP +LFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVETRDHTLTPAKLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSG++VPARERVLLIANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGEVVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGW FHILEFISVERKWE DE TM QMLSTFKDY+DP WLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWAFHILEFISVERKWEVDELTMHQMLSTFKDYYDPFWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFT+QKCIRSQKYAAE GLPIL+NVLLPKTKGF  C+ DLRKCLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTDQKCIRSQKYAAEKGLPILKNVLLPKTKGFRTCLHDLRKCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPL +IPTTENEVANWLM+TF  KDQLL+KFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLHHIPTTENEVANWLMNTFRHKDQLLEKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HEGTEGDLST+ CLV+IITVM+LTS+STY T FSSIWFKIYVSLACSFLAFATRFNIRPM
Sbjct: 301 HEGTEGDLSTLNCLVSIITVMLLTSISTYFTFFSSIWFKIYVSLACSFLAFATRFNIRPM 360

Query: 361 PIFGHKKSS 370
           PIFGHKKSS
Sbjct: 361 PIFGHKKSS 369

BLAST of Tan0000427 vs. ExPASy TrEMBL
Match: A0A6J1EU85 (1-acylglycerol-3-phosphate O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111437804 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.3e-198
Identity = 341/367 (92.92%), Postives = 354/367 (96.46%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVEMRD TLTP RLFRGLLCLLVLLLTAFMMLV+YGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVEMRDRTLTPARLFRGLLCLLVLLLTAFMMLVFYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLA+WPFLFEKINKTKVIFSG+MVPARERVL+IANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLAVWPFLFEKINKTKVIFSGEMVPARERVLIIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGWGFHILEFISVERKWE DESTMRQMLSTFKD HDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEVDESTMRQMLSTFKDDHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQK IRSQKYAAENGLPIL+N+LLPKTKGFH CVQDLR+CLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKRIRSQKYAAENGLPILKNLLLPKTKGFHTCVQDLRQCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIP+DNIPTTENEVANWLMDTFS KDQLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPIDNIPTTENEVANWLMDTFSNKDQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HEGTEGDLSTMKCLV+I T+MVLTSLSTYLT FSSIWFKIYV  ACS+LAFATRFNIRPM
Sbjct: 301 HEGTEGDLSTMKCLVSITTLMVLTSLSTYLTFFSSIWFKIYVLFACSYLAFATRFNIRPM 360

Query: 361 PIFGHKK 368
           PIF  KK
Sbjct: 361 PIFRRKK 367

BLAST of Tan0000427 vs. ExPASy TrEMBL
Match: A0A6J1C3M9 (1-acylglycerol-3-phosphate O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111007956 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 8.2e-197
Identity = 335/367 (91.28%), Postives = 354/367 (96.46%), Query Frame = 0

Query: 3   VKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTSFF 62
           VKDVE R H LTP RL RGLLCLLVL+LTAFMML+YYGFISAI VRVFSIHYSRK+TSFF
Sbjct: 8   VKDVETRHHALTPARLLRGLLCLLVLILTAFMMLIYYGFISAIIVRVFSIHYSRKATSFF 67

Query: 63  FGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQLGY 122
           FGSWLALWPFLFEKINKTKVIFSG+MVPA+ERVLLIANHRTEVDWMYLWDLA+RKGQLGY
Sbjct: 68  FGSWLALWPFLFEKINKTKVIFSGEMVPAKERVLLIANHRTEVDWMYLWDLAMRKGQLGY 127

Query: 123 IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG 182
           IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG
Sbjct: 128 IKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFPEG 187

Query: 183 TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHRCP 242
           TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGY+HRCP
Sbjct: 188 TDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYKHRCP 247

Query: 243 SLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFPHE 302
           SL+DNVFGLEPSEVHIHIQRIPL+NIPTTENEV NWLMD+FS+KDQLL+ F SQGHFP+E
Sbjct: 248 SLMDNVFGLEPSEVHIHIQRIPLNNIPTTENEVTNWLMDSFSVKDQLLENFSSQGHFPNE 307

Query: 303 GTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPMPI 362
           GTEGDLST+KC V+IITVMVL ++STYLT FSSIWFKIYVSLACS+LAFATRFNIRPMPI
Sbjct: 308 GTEGDLSTIKCFVSIITVMVLITISTYLTFFSSIWFKIYVSLACSYLAFATRFNIRPMPI 367

Query: 363 FGHKKSS 370
           F H+KSS
Sbjct: 368 FSHRKSS 374

BLAST of Tan0000427 vs. ExPASy TrEMBL
Match: A0A0A0K3N6 (1-acylglycerol-3-phosphate O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G253210 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.4e-190
Identity = 324/368 (88.04%), Postives = 348/368 (94.57%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKD++ RDHTLTP RLFRGLLCLLVLLLT FM+LVYYGFISAIFVRVFSIHYSRK+T+
Sbjct: 1   MEVKDIKTRDHTLTPARLFRGLLCLLVLLLTTFMILVYYGFISAIFVRVFSIHYSRKATA 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSG++VPARERVLLIANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGEVVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGW FHILEFISVERKWEADESTM QMLSTFKDYHDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWAFHILEFISVERKWEADESTMHQMLSTFKDYHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQKCIRSQK+AAE GLPIL+NVLLPKTKGFH CVQDLR+CLDAVYDVTIGY+H+
Sbjct: 181 EGTDFTEQKCIRSQKHAAEKGLPILKNVLLPKTKGFHMCVQDLRQCLDAVYDVTIGYKHQ 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPL +IPTTEN+V NWLM+TFS K+QLLDKF+SQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLHHIPTTENQVTNWLMNTFSRKNQLLDKFYSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSSIWFKIYVSLACSFLAFATRFNIRPM 360
           HE TEGDLST+  LV+IITV++LT++STY T FSSIWFKIYV LACSFLA+ T FNIRP 
Sbjct: 301 HERTEGDLSTLNFLVSIITVILLTTISTYFTFFSSIWFKIYVLLACSFLAYITHFNIRPT 360

Query: 361 PIFGHKKS 369
           P FGHKKS
Sbjct: 361 PFFGHKKS 368

BLAST of Tan0000427 vs. ExPASy TrEMBL
Match: A0A6J1JNF9 (1-acylglycerol-3-phosphate O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486054 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 4.0e-183
Identity = 314/334 (94.01%), Postives = 325/334 (97.31%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVEMRD T TP RLFRGLLCLLVLLLTAFMMLV+YGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVEMRDRTQTPARLFRGLLCLLVLLLTAFMMLVFYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLA+WPFLFEKINKTKVIFSG+MVPARERVL+IANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLAVWPFLFEKINKTKVIFSGEMVPARERVLIIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGWGFHILEFISVERKWE DESTMRQMLSTFKDYHDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEVDESTMRQMLSTFKDYHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQKCIRSQKYAAENGLPIL+N+LLPKTKGFH CVQDLRKCLDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKCIRSQKYAAENGLPILKNLLLPKTKGFHTCVQDLRKCLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFS KDQLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSNKDQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFS 335
           HEGTEGDLSTMKCLV+I T++VLTSLSTY T FS
Sbjct: 301 HEGTEGDLSTMKCLVSITTLLVLTSLSTYFTFFS 334

BLAST of Tan0000427 vs. ExPASy TrEMBL
Match: A0A1S3BGI2 (1-acylglycerol-3-phosphate O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC103489613 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 302/333 (90.69%), Postives = 320/333 (96.10%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           MEVKDVE RDHTL+P RLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRK+TS
Sbjct: 1   MEVKDVETRDHTLSPARLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKATS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSG+MVPARERVLLIANHRTEVDWMYLWDLAIRKGQL
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGEMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           GYIKYILKSSLMKLPVFGW FHILEFISVERKWEADESTM QMLS+FKDYHDPLWLALFP
Sbjct: 121 GYIKYILKSSLMKLPVFGWAFHILEFISVERKWEADESTMHQMLSSFKDYHDPLWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTDFTEQKCI+SQK+AAE GLPIL NVLLPKTKGFH CVQDLRK LDAVYDVTIGY+HR
Sbjct: 181 EGTDFTEQKCIQSQKHAAEKGLPILENVLLPKTKGFHLCVQDLRKSLDAVYDVTIGYKHR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPSLLDNVFGLEPSEVHIHIQRIPL +IPTTEN+VANWLM+TF+LK+QLLDKFHSQGHFP
Sbjct: 241 CPSLLDNVFGLEPSEVHIHIQRIPLHHIPTTENQVANWLMNTFTLKNQLLDKFHSQGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLF 334
            EGTEGDLST+ CLV+I TV++LT++STYLT F
Sbjct: 301 QEGTEGDLSTLNCLVSITTVILLTTISTYLTFF 333

BLAST of Tan0000427 vs. TAIR 10
Match: AT3G18850.1 (lysophosphatidyl acyltransferase 5 )

HSP 1 Score: 510.8 bits (1314), Expect = 9.4e-145
Identity = 245/370 (66.22%), Postives = 302/370 (81.62%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           ME K V   D  L+ +R+ RG++CL+VL+ TAFMML+++GF+SA+ +R+FSI YSRK  S
Sbjct: 1   MEKKSVPNSD-KLSLIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANHRTEVDWMY WDLA+RKGQ+
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANHRTEVDWMYFWDLALRKGQI 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +RQ++S+FKD  D LWLALFP
Sbjct: 121 GNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLRQIVSSFKDPRDALWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q+L   LDAVYDVTIGY+ R
Sbjct: 181 EGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQELSCSLDAVYDVTIGYKTR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+TF LKDQLL+ F+S GHFP
Sbjct: 241 CPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMNTFQLKDQLLNDFYSNGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKIYVSLACSFLAFATRFNIRP 360
           +EGTE + +T K L+  + V+  T++ T+LT FSS IWF+IYVSLAC +L  AT FN+R 
Sbjct: 301 NEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRIYVSLACVYLTSATHFNLRS 360

Query: 361 MPIFGHKKSS 370
           +P+    K+S
Sbjct: 361 VPLVETAKNS 369

BLAST of Tan0000427 vs. TAIR 10
Match: AT3G18850.2 (lysophosphatidyl acyltransferase 5 )

HSP 1 Score: 510.8 bits (1314), Expect = 9.4e-145
Identity = 245/370 (66.22%), Postives = 302/370 (81.62%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           ME K V   D  L+ +R+ RG++CL+VL+ TAFMML+++GF+SA+ +R+FSI YSRK  S
Sbjct: 1   MEKKSVPNSD-KLSLIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANHRTEVDWMY WDLA+RKGQ+
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANHRTEVDWMYFWDLALRKGQI 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +RQ++S+FKD  D LWLALFP
Sbjct: 121 GNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLRQIVSSFKDPRDALWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q+L   LDAVYDVTIGY+ R
Sbjct: 181 EGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQELSCSLDAVYDVTIGYKTR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+TF LKDQLL+ F+S GHFP
Sbjct: 241 CPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMNTFQLKDQLLNDFYSNGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKIYVSLACSFLAFATRFNIRP 360
           +EGTE + +T K L+  + V+  T++ T+LT FSS IWF+IYVSLAC +L  AT FN+R 
Sbjct: 301 NEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRIYVSLACVYLTSATHFNLRS 360

Query: 361 MPIFGHKKSS 370
           +P+    K+S
Sbjct: 361 VPLVETAKNS 369

BLAST of Tan0000427 vs. TAIR 10
Match: AT3G18850.3 (lysophosphatidyl acyltransferase 5 )

HSP 1 Score: 510.8 bits (1314), Expect = 9.4e-145
Identity = 245/370 (66.22%), Postives = 302/370 (81.62%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           ME K V   D  L+ +R+ RG++CL+VL+ TAFMML+++GF+SA+ +R+FSI YSRK  S
Sbjct: 1   MEKKSVPNSD-KLSLIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANHRTEVDWMY WDLA+RKGQ+
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANHRTEVDWMYFWDLALRKGQI 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +RQ++S+FKD  D LWLALFP
Sbjct: 121 GNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLRQIVSSFKDPRDALWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q+L   LDAVYDVTIGY+ R
Sbjct: 181 EGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQELSCSLDAVYDVTIGYKTR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+TF LKDQLL+ F+S GHFP
Sbjct: 241 CPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMNTFQLKDQLLNDFYSNGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKIYVSLACSFLAFATRFNIRP 360
           +EGTE + +T K L+  + V+  T++ T+LT FSS IWF+IYVSLAC +L  AT FN+R 
Sbjct: 301 NEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRIYVSLACVYLTSATHFNLRS 360

Query: 361 MPIFGHKKSS 370
           +P+    K+S
Sbjct: 361 VPLVETAKNS 369

BLAST of Tan0000427 vs. TAIR 10
Match: AT3G18850.4 (lysophosphatidyl acyltransferase 5 )

HSP 1 Score: 510.8 bits (1314), Expect = 9.4e-145
Identity = 245/370 (66.22%), Postives = 302/370 (81.62%), Query Frame = 0

Query: 1   MEVKDVEMRDHTLTPVRLFRGLLCLLVLLLTAFMMLVYYGFISAIFVRVFSIHYSRKSTS 60
           ME K V   D  L+ +R+ RG++CL+VL+ TAFMML+++GF+SA+ +R+FSI YSRK  S
Sbjct: 1   MEKKSVPNSD-KLSLIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVS 60

Query: 61  FFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANHRTEVDWMYLWDLAIRKGQL 120
           FFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANHRTEVDWMY WDLA+RKGQ+
Sbjct: 61  FFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANHRTEVDWMYFWDLALRKGQI 120

Query: 121 GYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMRQMLSTFKDYHDPLWLALFP 180
           G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +RQ++S+FKD  D LWLALFP
Sbjct: 121 GNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLRQIVSSFKDPRDALWLALFP 180

Query: 181 EGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQDLRKCLDAVYDVTIGYQHR 240
           EGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q+L   LDAVYDVTIGY+ R
Sbjct: 181 EGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQELSCSLDAVYDVTIGYKTR 240

Query: 241 CPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMDTFSLKDQLLDKFHSQGHFP 300
           CPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+TF LKDQLL+ F+S GHFP
Sbjct: 241 CPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMNTFQLKDQLLNDFYSNGHFP 300

Query: 301 HEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKIYVSLACSFLAFATRFNIRP 360
           +EGTE + +T K L+  + V+  T++ T+LT FSS IWF+IYVSLAC +L  AT FN+R 
Sbjct: 301 NEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRIYVSLACVYLTSATHFNLRS 360

Query: 361 MPIFGHKKSS 370
           +P+    K+S
Sbjct: 361 VPLVETAKNS 369

BLAST of Tan0000427 vs. TAIR 10
Match: AT3G18850.5 (lysophosphatidyl acyltransferase 5 )

HSP 1 Score: 475.3 bits (1222), Expect = 4.4e-134
Identity = 223/329 (67.78%), Postives = 270/329 (82.07%), Query Frame = 0

Query: 42  ISAIFVRVFSIHYSRKSTSFFFGSWLALWPFLFEKINKTKVIFSGDMVPARERVLLIANH 101
           + A+ +R+FSI YSRK  SFFFGSWLALWPFLFEKINKTKVIFSGD VP  +RVLLIANH
Sbjct: 18  LRAVVLRLFSIRYSRKCVSFFFGSWLALWPFLFEKINKTKVIFSGDKVPCEDRVLLIANH 77

Query: 102 RTEVDWMYLWDLAIRKGQLGYIKYILKSSLMKLPVFGWGFHILEFISVERKWEADESTMR 161
           RTEVDWMY WDLA+RKGQ+G IKY+LKSSLMKLP+FGW FH+ EFI VER+WE DE+ +R
Sbjct: 78  RTEVDWMYFWDLALRKGQIGNIKYVLKSSLMKLPLFGWAFHLFEFIPVERRWEVDEANLR 137

Query: 162 QMLSTFKDYHDPLWLALFPEGTDFTEQKCIRSQKYAAENGLPILRNVLLPKTKGFHACVQ 221
           Q++S+FKD  D LWLALFPEGTD+TE KC RS+K+AAENGLPIL NVLLP+TKGF +C+Q
Sbjct: 138 QIVSSFKDPRDALWLALFPEGTDYTEAKCQRSKKFAAENGLPILNNVLLPRTKGFVSCLQ 197

Query: 222 DLRKCLDAVYDVTIGYQHRCPSLLDNVFGLEPSEVHIHIQRIPLDNIPTTENEVANWLMD 281
           +L   LDAVYDVTIGY+ RCPS LDNV+G+EPSEVHIHI+RI L  IP  E ++  WLM+
Sbjct: 198 ELSCSLDAVYDVTIGYKTRCPSFLDNVYGIEPSEVHIHIRRINLTQIPNQEKDINAWLMN 257

Query: 282 TFSLKDQLLDKFHSQGHFPHEGTEGDLSTMKCLVTIITVMVLTSLSTYLTLFSS-IWFKI 341
           TF LKDQLL+ F+S GHFP+EGTE + +T K L+  + V+  T++ T+LT FSS IWF+I
Sbjct: 258 TFQLKDQLLNDFYSNGHFPNEGTEKEFNTKKYLINCLAVIAFTTICTHLTFFSSMIWFRI 317

Query: 342 YVSLACSFLAFATRFNIRPMPIFGHKKSS 370
           YVSLAC +L  AT FN+R +P+    K+S
Sbjct: 318 YVSLACVYLTSATHFNLRSVPLVETAKNS 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN41.3e-14366.22Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 OS=Arabidopsis thalian... [more]
Q8L4Y27.3e-12659.72Probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 4 OS=Arabidopsis thalian... [more]
Q5F3X04.4e-4634.38Lysocardiolipin acyltransferase 1 OS=Gallus gallus OX=9031 GN=LCLAT1 PE=2 SV=1[more]
Q9XFW44.4e-4637.741-acyl-sn-glycerol-3-phosphate acyltransferase 2 OS=Brassica napus OX=3708 GN=LP... [more]
Q6IWY14.4e-4637.841-acyl-sn-glycerol-3-phosphate acyltransferase 2 OS=Brassica oleracea OX=3712 GN... [more]
Match NameE-valueIdentityDescription
XP_023530104.11.3e-20194.02probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita ... [more]
KAG7022423.19.6e-20093.22putative 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Cucurbita argyrosperm... [more]
XP_022931644.16.9e-19892.92probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 isoform X1 [Cucurbita ... [more]
XP_022136204.11.7e-19691.28probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Momordica charantia][more]
XP_038887315.13.8e-19691.06probable 1-acyl-sn-glycerol-3-phosphate acyltransferase 5 [Benincasa hispida] >X... [more]
Match NameE-valueIdentityDescription
A0A6J1EU853.3e-19892.921-acylglycerol-3-phosphate O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1C3M98.2e-19791.281-acylglycerol-3-phosphate O-acyltransferase OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0K3N64.4e-19088.041-acylglycerol-3-phosphate O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7... [more]
A0A6J1JNF94.0e-18394.011-acylglycerol-3-phosphate O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3BGI22.3e-17590.691-acylglycerol-3-phosphate O-acyltransferase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT3G18850.19.4e-14566.22lysophosphatidyl acyltransferase 5 [more]
AT3G18850.29.4e-14566.22lysophosphatidyl acyltransferase 5 [more]
AT3G18850.39.4e-14566.22lysophosphatidyl acyltransferase 5 [more]
AT3G18850.49.4e-14566.22lysophosphatidyl acyltransferase 5 [more]
AT3G18850.54.4e-13467.78lysophosphatidyl acyltransferase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002123Phospholipid/glycerol acyltransferaseSMARTSM00563plsc_2coord: 95..217
e-value: 5.0E-26
score: 102.5
IPR002123Phospholipid/glycerol acyltransferasePFAMPF01553Acyltransferasecoord: 84..237
e-value: 4.7E-21
score: 74.8
IPR032098Acyltransferase, C-terminal domainPFAMPF16076Acyltransf_Ccoord: 249..310
e-value: 3.1E-18
score: 65.6
NoneNo IPR availablePANTHERPTHR10983:SF571-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 5-RELATEDcoord: 6..368
NoneNo IPR availablePANTHERPTHR109831-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATEDcoord: 6..368
NoneNo IPR availableCDDcd07990LPLAT_LCLAT1-likecoord: 71..261
e-value: 6.31966E-84
score: 251.389
NoneNo IPR availableSUPERFAMILY69593Glycerol-3-phosphate (1)-acyltransferasecoord: 28..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000427.1Tan0000427.1mRNA
Tan0000427.2Tan0000427.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016024 CDP-diacylglycerol biosynthetic process
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003841 1-acylglycerol-3-phosphate O-acyltransferase activity
molecular_function GO:0016746 acyltransferase activity