Tan0000376 (gene) Snake gourd v1

Overview


Name	Tan0000376
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Allene oxide synthase
Location	LG02: 9374226 .. 9377283 (-)
RNA-Seq Expression	Tan0000376
Synteny	Tan0000376

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTGTCATGTTTTTATTATTTTTATTATCTTGGTTTTTAATTGTATCATAGATTCATGAATATCACCATCCACCCCTCCCCACAAAAATATATTGTTTTTTTTAGCATAAAAACTGGGAAAAAACTTTGTTTTTGTTTGAACAAGCATCGACCAAAAAAACTCTCCCCCCAAAAAAGAAAAATGGCCTCTTCTTCCTCTGAGCTTGCTCTGAAATCCATTCCCGGCGGCTATGGTTTCTCCGTCTTCGGAGCGATCAAAGATCGATACGACTACTTCTACATCCAAGGAAAAGACGAATTTTTCCGATCCCGAATGACCAAATATCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCATCGTCCTTCTCGACGCCGTCAGCTTTCAGATCCTCTTTGACACCTCTAAGATCGAAAAACGCAATGTTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGTGACATTCGCACCTGCGCCTATTTGGACCCATCGGAAACAGAGCACTCTGTTCTCAAGAAGCTCTTCCTCTCTTTTCTCGCCTCTCGTCACGACAGGTTTATCCCTCTCTTACGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAACTGGCGGAAAGGAAGAAGGTCGCCGATTTCAACCCGATTAGTGATTCCATGTCGTTTGATTATATTTTCCGGCTACTCTCCGACGGGACCCCTGACTCTAAACTGGCCGCCAGTGGACCTGGATTGTTCGATAAATGGCTCACCTTCCAGCTCGCACCACTGGCTTCCATTGGCTTGCCGAAGATCTTTTCAGTTTTTGAAGATCTCATCATCCACACCATTCCGCTGCCTTTCTTTCTCGTCAAAAGTGGTTACAGGTAGAAGAAGATCGAGCGATTTCTGTTCTTTTGGGGTTTTTTTGGGGGGGGGTTAAATTTTGAAGTTCATTTTTTGAATTTGGTTTTACAGGAAGCTCTACGATGCGTTTTACTCCTCCTCTGGTTCGTTTCTGGCTGAAGCGGAGAAACAGGGGATAGACAGAGAAAAAGCGTGTCACAATTTAGTGTTTCTGGCCGGATTCAACGCGTACGGCGGAATGAAAGTACTTTTCCCGATTTTGCTGAAGTGGGTCGGCACCGCCGGCGATGGTCTCCACCGGAAGCTCGCCGATGAGGTCCGAGCCGCCGTGAAGGAAGAAGGGGGATTGACTTTTGGCGCGATAGAGAAGATGAGTCTGTTGAAATCCGTCGTGTACGAAGCTCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGGGAAGGCGAAGGAGGACATCGTGATTCATAGCCACGATTCCGCTTTCAAGATCAAGAAAGGGGAGACGATTTTTGGGTATCAGCCGTTTGCCACTAATGATCCGAAGATCTTTGAGAATCCGGAGAAGTTCGTCGGCGATCGGTTCGTCGGAGAAGGGGAGAAGCTTCTGAAGTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGCCGGAGGACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGTTGATGGCCAGGGTCCTTCTGGTGGAATTCTTCCTCCGTTATGACACGTTCTCCGTCGACGTCGCTAACTTGCCGCTCGGTCCGTCGGTGAAAATCAAATCCGTGACCAAAGCCCCTGCCAAAGTTTGAAGCTACTTATGCAAATTGGTCCCTTTTCCTTTTCAGGTAAATTTTTTAAAATCTCTGTTTAAAATTTTTGAAAGCTTTCTAAATAGATTATAATAAGACGTTTATAATAATAGTACGGGAAATTATATAGTTAAAATGTGGAGTTCTTTGATCTGTTTTTGAGTTCTATTACAAGTTTTTCGAGAGGGTAATAGCTAGGAATCTTTAAGTCCCTAACTTTCCTTAGGTAGAGAGATTTTAACATATTTACTAGTATGCAATCAAGTATTATTCTTTTAATAAATAGTTGTTAACCAGAAGTTGATATTTCTTAAATTATCTTATTGTTTTATAAATATATTTAGACACATGAAATTGAATATATTAATAAACAAAATTTGCTTCTTTTAGTATTAGGTTCACACGTTCAAATAGGTGAAAAACAGTTAAAATATAGTTTAATTGGATAATGTATTATAATCCGACTAAAAAGGTTAAAAACAATATTTTTTTTTTACATTTAATAAATAAATGAATATTTTTAATATTTTTAACTAATAAAATATGTCACGTTAGAAAGCAAGTAGGAACTCATACTTTTTTTTAGCTCAATAATTGCAAAAGTTAAGAATCAAATTATTTATTTTTAGAATCGTAATTGATATCTTATCTAATAAATTATGCTCGAATTGATATAACAACTCTTAGGGATTGTTTGAGACACTAATGAGTTATAATAATAGCGATTATAATAGTATATAGGTTATTATAATCTTTGAAATCATATAATATTATTTAAAGTGTAAAGTAGTATAGTCTGAAATTATAATAGTTCATGTTTGGGGTGCAGAATTTTCTACAAATTATAATAACATGTGCTCCAAATAAACTCATACTAACTAGTAAGTATCATTTATATGTCAATTAAATAATAATTTAATAACATGTGAAATGTTCTTTCGCTTAAAATTTTGAGTGCTACTGGTCAACTTTTCATTATTGAATTTTTTATATCTGATTAAGAACTAAGAATTTAAAAAGATAAAAGGGCAAATTCATAATTATCTAAAAAGCTAGGGACTATTCAGCATATTTAGACAAAGTTTGTTGATGATTGTCAGAAATGAGTGAAGGAAGTTCATGATTGGTAGATCGGATGCACGCGGGCTGTCGTGGACTTGAGCACACATGATTGAGCTGTGGGGTTAAAGAGTACGTTCCTCGTGGTCCACGTGCTTGTATCTTAACAGCGATTTCTGTAAGCTTTAATTATGTGAGTGTTGTAGAGGATCTTATTTCTAATAGTTTGTTTTGTGAATTGTATACTCTAATAAATAAATAAATAAATCTATCTTGTTTATTTTCATTAAA

mRNA sequence

ATTTTGTCATGTTTTTATTATTTTTATTATCTTGGTTTTTAATTGTATCATAGATTCATGAATATCACCATCCACCCCTCCCCACAAAAATATATTGTTTTTTTTAGCATAAAAACTGGGAAAAAACTTTGTTTTTGTTTGAACAAGCATCGACCAAAAAAACTCTCCCCCCAAAAAAGAAAAATGGCCTCTTCTTCCTCTGAGCTTGCTCTGAAATCCATTCCCGGCGGCTATGGTTTCTCCGTCTTCGGAGCGATCAAAGATCGATACGACTACTTCTACATCCAAGGAAAAGACGAATTTTTCCGATCCCGAATGACCAAATATCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCATCGTCCTTCTCGACGCCGTCAGCTTTCAGATCCTCTTTGACACCTCTAAGATCGAAAAACGCAATGTTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGTGACATTCGCACCTGCGCCTATTTGGACCCATCGGAAACAGAGCACTCTGTTCTCAAGAAGCTCTTCCTCTCTTTTCTCGCCTCTCGTCACGACAGGTTTATCCCTCTCTTACGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAACTGGCGGAAAGGAAGAAGGTCGCCGATTTCAACCCGATTAGTGATTCCATGTCGTTTGATTATATTTTCCGGCTACTCTCCGACGGGACCCCTGACTCTAAACTGGCCGCCAGTGGACCTGGATTGTTCGATAAATGGCTCACCTTCCAGCTCGCACCACTGGCTTCCATTGGCTTGCCGAAGATCTTTTCAGTTTTTGAAGATCTCATCATCCACACCATTCCGCTGCCTTTCTTTCTCGTCAAAAGTGGTTACAGGAAGCTCTACGATGCGTTTTACTCCTCCTCTGGTTCGTTTCTGGCTGAAGCGGAGAAACAGGGGATAGACAGAGAAAAAGCGTGTCACAATTTAGTGTTTCTGGCCGGATTCAACGCGTACGGCGGAATGAAAGTACTTTTCCCGATTTTGCTGAAGTGGGTCGGCACCGCCGGCGATGGTCTCCACCGGAAGCTCGCCGATGAGGTCCGAGCCGCCGTGAAGGAAGAAGGGGGATTGACTTTTGGCGCGATAGAGAAGATGAGTCTGTTGAAATCCGTCGTGTACGAAGCTCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGGGAAGGCGAAGGAGGACATCGTGATTCATAGCCACGATTCCGCTTTCAAGATCAAGAAAGGGGAGACGATTTTTGGGTATCAGCCGTTTGCCACTAATGATCCGAAGATCTTTGAGAATCCGGAGAAGTTCGTCGGCGATCGGTTCGTCGGAGAAGGGGAGAAGCTTCTGAAGTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGCCGGAGGACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGTTGATGGCCAGGGTCCTTCTGGTGGAATTCTTCCTCCGTTATGACACGTTCTCCGTCGACGTCGCTAACTTGCCGCTCGGTCCGTCGGTGAAAATCAAATCCGTGACCAAAGCCCCTGCCAAAGTTTGAAGCTACTTATGCAAATTGGTCCCTTTTCCTTTTCAGAAATGAGTGAAGGAAGTTCATGATTGGTAGATCGGATGCACGCGGGCTGTCGTGGACTTGAGCACACATGATTGAGCTGTGGGGTTAAAGAGTACGTTCCTCGTGGTCCACGTGCTTGTATCTTAACAGCGATTTCTGTAAGCTTTAATTATGTGAGTGTTGTAGAGGATCTTATTTCTAATAGTTTGTTTTGTGAATTGTATACTCTAATAAATAAATAAATAAATCTATCTTGTTTATTTTCATTAAA

Coding sequence (CDS)

ATGAATATCACCATCCACCCCTCCCCACAAAAATATATTGTTTTTTTTAGCATAAAAACTGGGAAAAAACTTTGTTTTTGTTTGAACAAGCATCGACCAAAAAAACTCTCCCCCCAAAAAAGAAAAATGGCCTCTTCTTCCTCTGAGCTTGCTCTGAAATCCATTCCCGGCGGCTATGGTTTCTCCGTCTTCGGAGCGATCAAAGATCGATACGACTACTTCTACATCCAAGGAAAAGACGAATTTTTCCGATCCCGAATGACCAAATATCGCTCCACCGTCTTCCGCGCCAACATGCCGCCGGGCCCCTTCATCTCCTCCGACTCCAGAGTCATCGTCCTTCTCGACGCCGTCAGCTTTCAGATCCTCTTTGACACCTCTAAGATCGAAAAACGCAATGTTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGTGACATTCGCACCTGCGCCTATTTGGACCCATCGGAAACAGAGCACTCTGTTCTCAAGAAGCTCTTCCTCTCTTTTCTCGCCTCTCGTCACGACAGGTTTATCCCTCTCTTACGAAGCTCCTTGTCGGAGATGTTTATTAAACTTGAAGATAAACTGGCGGAAAGGAAGAAGGTCGCCGATTTCAACCCGATTAGTGATTCCATGTCGTTTGATTATATTTTCCGGCTACTCTCCGACGGGACCCCTGACTCTAAACTGGCCGCCAGTGGACCTGGATTGTTCGATAAATGGCTCACCTTCCAGCTCGCACCACTGGCTTCCATTGGCTTGCCGAAGATCTTTTCAGTTTTTGAAGATCTCATCATCCACACCATTCCGCTGCCTTTCTTTCTCGTCAAAAGTGGTTACAGGAAGCTCTACGATGCGTTTTACTCCTCCTCTGGTTCGTTTCTGGCTGAAGCGGAGAAACAGGGGATAGACAGAGAAAAAGCGTGTCACAATTTAGTGTTTCTGGCCGGATTCAACGCGTACGGCGGAATGAAAGTACTTTTCCCGATTTTGCTGAAGTGGGTCGGCACCGCCGGCGATGGTCTCCACCGGAAGCTCGCCGATGAGGTCCGAGCCGCCGTGAAGGAAGAAGGGGGATTGACTTTTGGCGCGATAGAGAAGATGAGTCTGTTGAAATCCGTCGTGTACGAAGCTCTGAGGATCGAGCCGCCGGTGCCGTTCCAGTACGGGAAGGCGAAGGAGGACATCGTGATTCATAGCCACGATTCCGCTTTCAAGATCAAGAAAGGGGAGACGATTTTTGGGTATCAGCCGTTTGCCACTAATGATCCGAAGATCTTTGAGAATCCGGAGAAGTTCGTCGGCGATCGGTTCGTCGGAGAAGGGGAGAAGCTTCTGAAGTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGCCGGAGGACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGTTGATGGCCAGGGTCCTTCTGGTGGAATTCTTCCTCCGTTATGACACGTTCTCCGTCGACGTCGCTAACTTGCCGCTCGGTCCGTCGGTGAAAATCAAATCCGTGACCAAAGCCCCTGCCAAAGTTTGA

Protein sequence

MNITIHPSPQKYIVFFSIKTGKKLCFCLNKHRPKKLSPQKRKMASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPFISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLLSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYRKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDGLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKLLKYVYWSNGRETEEPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV

Homology

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BLAST of Tan0000376 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.1e-159
Identity = 270/473 (57.08%), Postives = 353/473 (74.63%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           SS   L +K IPG YG   FGAIKDRYD+ Y QG DEFFRSRM KY STVFR N+PPGPF
Sbjct: 18  SSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGPF 77

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
            + +S+V+VL+DAVS+ ILFD S+++K N  +GT+M S SF G  + C +L  S+ +H+ 
Sbjct: 78  NARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHTT 137

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LK LFLS L   HD+FIP+  +S++ MF  LE +L+E K  + FNPI D++SF+++FRL 
Sbjct: 138 LKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSE-KGTSYFNPIGDNLSFEFLFRLF 197

Query: 225 SDGTP--DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSG 284
            +G    D+ +  +GP + DKW+  QLAPL S+GL  + +  EDL++HT PLP+ LVK  
Sbjct: 198 CEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRD 257

Query: 285 YRKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAG 344
           ++KLY+AFY+S    L EAEK G+ R++ACHN VFLAGFN+YGG+KV FP L+KW+GT+G
Sbjct: 258 HQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSG 317

Query: 345 DGLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIH 404
             LH +L  E+R AVKE GG+T  AI+KM L+KSVVYE LR++PPVPFQ  KA+++I+I 
Sbjct: 318 PSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIIT 377

Query: 405 SHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKLLKYVYWSNGRETEE 464
           +H+S+F IKK E IFGYQP AT D K+F+N E+F  DRFVG GEKLLKYVYWSNG+E + 
Sbjct: 378 NHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVGGGEKLLKYVYWSNGKEIDN 437

Query: 465 PTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
           P+  DKQCPGK+L+VLM R+L+VEFF+RYDTF V+   L LG  V  KS+TKA
Sbjct: 438 PSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKA 489

[Close]

BLAST of Tan0000376 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.5e-142
Identity = 257/487 (52.77%), Postives = 344/487 (70.64%), Query Frame = 0


Query: 37  SPQKRKMASSS-----SELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYR 96
           SP +   +SSS     ++L  + +PG YG  + G  KDR DYFY QGK+EFF+SR+ K++
Sbjct: 50  SPSQSPSSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQ 109

Query: 97  STVFRANMPPGPFISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRT 156
           STVFR NMPPGPFIS +  V+VLLD  SF +LFD SK+EK+++  GT+MPS   TG  R 
Sbjct: 110 STVFRTNMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRV 169

Query: 157 CAYLDPSETEHSVLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPI 216
            +YLDPSE  H+ LKKL    L+SR +  IP   +S SE+F  LE++L+ + K A  N  
Sbjct: 170 LSYLDPSEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGK-AGLNAA 229

Query: 217 SDSMSFDYIFRLLSDGTP-DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIH 276
           +D  +F+++ R L    P D++L   GP L  KW+ FQL PL  +GLPK   V EDL++H
Sbjct: 230 NDQAAFNFLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPK---VLEDLVMH 289

Query: 277 TIPLPFFLVKSGYRKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVL 336
           T  LP  LVK  Y++LY+ FY +S S L EAEK GI RE+ACHNL+F   FN++GG+K+ 
Sbjct: 290 TFRLPPALVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIF 349

Query: 337 FPILLKWVGTAGDGLHRKLADEVRAAVKEEGG-LTFGAIEKMSLLKSVVYEALRIEPPVP 396
           FP +LKW+G AG  LH +LA E+R+ +    G +T  A+EKM L+KSVVYE+LRIEPPV 
Sbjct: 350 FPNMLKWIGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVA 409

Query: 397 FQYGKAKEDIVIHSHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKL 456
            QYG+AK D+VI SHD++F+IK+GE ++GYQPFAT DPKIF+  E+FV DRF+G EGEKL
Sbjct: 410 SQYGRAKHDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKL 469

Query: 457 LKYVYWSNGRETEEPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVK 516
           LK+V WSNG ETE  +  +KQC GK+ VVL++R+LLVE FLRYD+F ++V   PLG ++ 
Sbjct: 470 LKHVLWSNGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAIT 529

[Close]

BLAST of Tan0000376 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 502.7 bits (1293), Expect = 5.1e-141
Identity = 251/474 (52.95%), Postives = 331/474 (69.83%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           + S +L +++IPG YG  + G IKDR+DYFY QG +EFF+SR+ KY STV+R NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           I+ + +V+ LLD  SF +LFD  K+EK+++  GTYMPS   TG  R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LK L    L S  +R  P  +++ SE+F  LE +L+ + K ADF   SD  +F+++ R  
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 225 SDGTP-DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 284
               P D+KL A  PGL  KW+ F L PL SIGLP+   V E+ +IHT  LP  LVKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 285 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 344
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMK+LFP ++K +G AG 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 345 GLHRKLADEVRAAVKEEGG-LTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIH 404
            +H +LA+E+R+ +K  GG LT GAIEKM L KSVVYE LR EPPV  QYG+AK+D+VI 
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 405 SHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETE 464
           SHD+AFK+K GE ++GYQP AT DPKIF+  ++FV +RFVG EGEKLL++V WSNG ETE
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 465 EPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
            PT  +KQC GK+ VVL+AR+ ++E F RYD+F ++V   PLG SV   S+ KA
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

[Close]

BLAST of Tan0000376 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.9e-140
Identity = 248/470 (52.77%), Postives = 330/470 (70.21%), Query Frame = 0


Query: 49  ELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPFISSD 108
           +L+ ++IPG YG    G  KDR DYFY QGK++FF SR+ KY+ST+FR NMPPGPFI+S+
Sbjct: 45  KLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSN 104

Query: 109 SRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKL 168
            +VIVLLD  SF +LFD SK+EK+++  GT++PS   TG  R  +YLDPSE  H  LKKL
Sbjct: 105 PKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKL 164

Query: 169 FLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLLSDGT 228
               L+SR D  IP    + +E+F  L DK  E K    FN  SD  +F+++ R L    
Sbjct: 165 MFFLLSSRRDHVIPEFHETYTELFETL-DKEMEEKGTVGFNSGSDQAAFNFLARSLFGVN 224

Query: 229 P-DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYRKLY 288
           P ++KL   GP L  KW+  QL P+ ++GLPK     +D+++HT  LP  LVK  Y++LY
Sbjct: 225 PVETKLGTDGPALIGKWILLQLHPVITLGLPKF---LDDVLLHTFRLPPILVKKDYQRLY 284

Query: 289 DAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDGLHR 348
           D FY++S +   EAEK GI +++ACHNL+F   FN++GGMK+ FP +LK +  AG  +H 
Sbjct: 285 DFFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHT 344

Query: 349 KLADEVRAAVKEEGG-LTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSHDS 408
           +LA+E+R+ VK  GG +T  A+EKM L+KSVVYEALR++PPV  QYG+AK+D+ I SHD+
Sbjct: 345 RLANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDA 404

Query: 409 AFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEPTP 468
            F++KKGE +FGYQPFAT DPKIF+ P +FV DRFVG EGEKLLK+V WSNG ETE PT 
Sbjct: 405 VFEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTV 464

Query: 469 EDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
            +KQC GK+ VV+++R+ + EFFLRY T +VDV    LG S+ I S+ KA
Sbjct: 465 GNKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

[Close]

BLAST of Tan0000376 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.6e-140
Identity = 242/475 (50.95%), Postives = 337/475 (70.95%), Query Frame = 0


Query: 44  ASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGP 103
           +S  + L ++ IPG YG    G I+DR DYFY QG++EFF+SR+ KY+STV+RANMPPGP
Sbjct: 64  SSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGP 123

Query: 104 FISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHS 163
           FI+S+ RVIVLLDA SF +LFD SK+EK+++  GTYMPS   TG  R  +YLDPSE  H+
Sbjct: 124 FIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHT 183

Query: 164 VLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRL 223
            LK+L  + + +R D  IP   SS +++   +E  LA + K A FN  ++  +F+++ R 
Sbjct: 184 KLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAA-FNDPAEQAAFNFLSRA 243

Query: 224 LSDGTP-DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSG 283
                P D+ L    P L  KW+ F LAP+ S+GLPK     E+  +H++ LP  LV++ 
Sbjct: 244 FFGVKPIDTPLGKDAPSLISKWVLFNLAPILSVGLPK---EVEEATLHSVRLPPLLVQND 303

Query: 284 YRKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAG 343
           Y +LY+ F S++GS L EAE+ GI R++ACHN++F   FN++GG K+LFP L+KW+G AG
Sbjct: 304 YHRLYEFFTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGRAG 363

Query: 344 DGLHRKLADEVRAAVKEEGG--LTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIV 403
             LH KLA E+R+A++  GG  +T  A+E+M L+KSVVYE LRIEPPV  QYGKAK+D +
Sbjct: 364 LELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFI 423

Query: 404 IHSHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKLLKYVYWSNGRET 463
           + SH++A+++K+GE +FGYQPFAT DPKIF+ PE+FV DRFVGEG KL++YV WSNG ET
Sbjct: 424 LESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGEGVKLMEYVMWSNGPET 483

Query: 464 EEPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
           E P+  +KQC GK+ VV+ AR+ +VE F RYD+F ++V    LG S+ + S+ ++
Sbjct: 484 ETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRS 534

[Close]

BLAST of Tan0000376 vs. NCBI nr
Match: XP_022988758.1 (allene oxide synthase 3-like [Cucurbita maxima] >XP_022988759.1 allene oxide synthase 3-like [Cucurbita maxima])

HSP 1 Score: 859.8 bits (2220), Expect = 1.2e-245
Identity = 424/476 (89.08%), Postives = 449/476 (94.33%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           SSSSEL LK IPGGYGF   GAIKDRYDYFY QGKDEFFRSR+TKYRSTVFRANMPPGPF
Sbjct: 5   SSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVTKYRSTVFRANMPPGPF 64

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           ISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETEHSV
Sbjct: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LKK FL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IFRLL
Sbjct: 125 LKKHFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184

Query: 225 SDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYR 284
           SDGTPDSKLAA GPG+FDKWLTFQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKSGYR
Sbjct: 185 SDGTPDSKLAADGPGMFDKWLTFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYR 244

Query: 285 KLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDG 344
           KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAG+ 
Sbjct: 245 KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGEK 304

Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+ SH
Sbjct: 305 LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSH 364

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEP 464
           DSAFKIKKGETIFGYQPFAT D ++FENPEKF+GDRFVG EGEKLLKYVYWSNGRETEEP
Sbjct: 365 DSAFKIKKGETIFGYQPFATKDGRVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEP 424

Query: 465 TPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           TPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA AKV
Sbjct: 425 TPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAAKV 480

[Close]

BLAST of Tan0000376 vs. NCBI nr
Match: KAG6588631.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022428.1 Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854.4 bits (2206), Expect = 5.2e-244
Identity = 424/479 (88.52%), Postives = 448/479 (93.53%), Query Frame = 0


Query: 43  MAS-SSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPP 102
           MAS SSSEL LKSIPGGYGF   GAIKDRYDYFY QGKDEFFRSR+ KYRSTVFRANMPP
Sbjct: 1   MASPSSSELPLKSIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPP 60

Query: 103 GPFISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETE 162
           GPFISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETE
Sbjct: 61  GPFISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETE 120

Query: 163 HSVLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIF 222
           HSVLKKLFL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IF
Sbjct: 121 HSVLKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIF 180

Query: 223 RLLSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKS 282
           RLLSDGTPDSKLAA GPG+FDKWL FQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKS
Sbjct: 181 RLLSDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKS 240

Query: 283 GYRKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTA 342
           GY KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKWVGTA
Sbjct: 241 GYMKLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTA 300

Query: 343 GDGLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI 402
           G+ LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+
Sbjct: 301 GEELHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVV 360

Query: 403 HSHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRET 462
            SHDSAFKIKKGE IFGYQPFAT D K+FENPEKF+GDRFVG EGEKLLKYVYWSNGRET
Sbjct: 361 QSHDSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRET 420

Query: 463 EEPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           EEPTPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA  KV
Sbjct: 421 EEPTPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 479

[Close]

BLAST of Tan0000376 vs. NCBI nr
Match: XP_022931572.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 853.2 bits (2203), Expect = 1.2e-243
Identity = 420/476 (88.24%), Postives = 445/476 (93.49%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           SSSSEL LK IPGGYGF   GAIKDRYDYFY QGKDEFFRSR+ KYRSTVFRANMPPGPF
Sbjct: 5   SSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPF 64

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           ISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETEHSV
Sbjct: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LKKLFL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IFRLL
Sbjct: 125 LKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184

Query: 225 SDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYR 284
           SDGTPDSKLAA GPG+FDKWL FQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKSGY 
Sbjct: 185 SDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYM 244

Query: 285 KLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDG 344
           KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKW+GTAG+ 
Sbjct: 245 KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAGEE 304

Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+ SH
Sbjct: 305 LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSH 364

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEP 464
           DSAFKIKKGE IFGYQPFAT D K+FENPEKF+GDRFVG EGEKLLKYVYWSNGRETEEP
Sbjct: 365 DSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEP 424

Query: 465 TPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           TPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA  KV
Sbjct: 425 TPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480

[Close]

BLAST of Tan0000376 vs. NCBI nr
Match: XP_023530097.1 (allene oxide synthase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.6e-243
Identity = 420/476 (88.24%), Postives = 445/476 (93.49%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           +SSSEL LKSIPGGYGF   GAIKDRYDYFY QGKDEFFRSR+ KYRSTVFRANMPPGPF
Sbjct: 5   TSSSELPLKSIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPF 64

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           ISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETEHSV
Sbjct: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LKKLFL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IFRLL
Sbjct: 125 LKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184

Query: 225 SDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYR 284
           SDGTPDSKLAA GPG+FDKWL FQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKSGY 
Sbjct: 185 SDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYM 244

Query: 285 KLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDG 344
           KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAG+ 
Sbjct: 245 KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGEE 304

Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+ SH
Sbjct: 305 LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSH 364

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEP 464
           DSAFKIKKGE IFGYQPFAT D K+FENPEKF+GDRFVG EGEKLLKYVYWSNGRETEEP
Sbjct: 365 DSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEP 424

Query: 465 TPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           TPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA   V
Sbjct: 425 TPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVTV 480

[Close]

BLAST of Tan0000376 vs. NCBI nr
Match: XP_038886895.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 839.0 bits (2166), Expect = 2.3e-239
Identity = 414/477 (86.79%), Postives = 439/477 (92.03%), Query Frame = 0


Query: 44  ASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGP 103
           +SSS EL LK IPGGYGFS  G IKDRYDYFY QGKDEFFRSR+TKY STVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFSFLGPIKDRYDYFYFQGKDEFFRSRVTKYGSTVFRANMPPGP 64

Query: 104 FISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHS 163
           FISSDSRV+VLLDAVSF ILFDT+K+EKRN+LDGTYMPSLSFTG IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDAVSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 124

Query: 164 VLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRL 223
           VLK LFLSFLASRHDR IPL RSSLSEMF+KLEDKLAE+KK+ADFNPISDSMSFDY+FRL
Sbjct: 125 VLKGLFLSFLASRHDRIIPLFRSSLSEMFVKLEDKLAEKKKIADFNPISDSMSFDYVFRL 184

Query: 224 LSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 283
            SDG PD KLAA GPG+FD WL FQLAPLASIG+P IFS+FEDLIIHTIPLPFF VKSGY
Sbjct: 185 FSDGNPDPKLAADGPGMFDLWLAFQLAPLASIGVPNIFSIFEDLIIHTIPLPFFPVKSGY 244

Query: 284 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 343
           RKLY AFYSSSGSFL EAEKQGIDREKACHNLVFLAGFNAYGGMKVLFP +LKWVGTAG+
Sbjct: 245 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTAGE 304

Query: 344 GLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 403
            LHRKLADEVR  VKEEGGLTFGA+EKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS
Sbjct: 305 DLHRKLADEVRTTVKEEGGLTFGALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 364

Query: 404 HDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEE 463
           HDSAFKIKKGETIFGYQPFAT DPKIF++ EKFVGDRF G EGEKLLKYVYWSN RET E
Sbjct: 365 HDSAFKIKKGETIFGYQPFATKDPKIFKDAEKFVGDRFEGEEGEKLLKYVYWSNERETVE 424

Query: 464 PTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           PT E+KQCPGKNLVVLM R++LVEFFLRYDTF+VDVA+LPLGP+VK KS+TKA  KV
Sbjct: 425 PTAENKQCPGKNLVVLMGRIILVEFFLRYDTFTVDVADLPLGPAVKFKSLTKATVKV 481

[Close]

BLAST of Tan0000376 vs. ExPASy TrEMBL
Match: A0A6J1JN86 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486003 PE=4 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.0e-246
Identity = 424/476 (89.08%), Postives = 449/476 (94.33%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           SSSSEL LK IPGGYGF   GAIKDRYDYFY QGKDEFFRSR+TKYRSTVFRANMPPGPF
Sbjct: 5   SSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVTKYRSTVFRANMPPGPF 64

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           ISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETEHSV
Sbjct: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LKK FL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IFRLL
Sbjct: 125 LKKHFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184

Query: 225 SDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYR 284
           SDGTPDSKLAA GPG+FDKWLTFQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKSGYR
Sbjct: 185 SDGTPDSKLAADGPGMFDKWLTFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYR 244

Query: 285 KLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDG 344
           KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAG+ 
Sbjct: 245 KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGEK 304

Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+ SH
Sbjct: 305 LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSH 364

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEP 464
           DSAFKIKKGETIFGYQPFAT D ++FENPEKF+GDRFVG EGEKLLKYVYWSNGRETEEP
Sbjct: 365 DSAFKIKKGETIFGYQPFATKDGRVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEP 424

Query: 465 TPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           TPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA AKV
Sbjct: 425 TPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAAKV 480

[Close]

BLAST of Tan0000376 vs. ExPASy TrEMBL
Match: A0A6J1EU14 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111437746 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.7e-244
Identity = 420/476 (88.24%), Postives = 445/476 (93.49%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           SSSSEL LK IPGGYGF   GAIKDRYDYFY QGKDEFFRSR+ KYRSTVFRANMPPGPF
Sbjct: 5   SSSSELPLKPIPGGYGFPFLGAIKDRYDYFYFQGKDEFFRSRVAKYRSTVFRANMPPGPF 64

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           ISSDSRV+VLLDA++FQILFDTSK+EKRN+LDGTYMPSLSFTGDIRTCAYLDPSETEHSV
Sbjct: 65  ISSDSRVVVLLDALTFQILFDTSKVEKRNLLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 124

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LKKLFL FLASRHDRFIPL RSSLSEMFIKLEDK+AE+ KV DFNPISDSMSFD+IFRLL
Sbjct: 125 LKKLFLCFLASRHDRFIPLFRSSLSEMFIKLEDKIAEKNKVVDFNPISDSMSFDFIFRLL 184

Query: 225 SDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYR 284
           SDGTPDSKLAA GPG+FDKWL FQLAPLASIGLPKIFS+ EDL+IHT+PLPFFLVKSGY 
Sbjct: 185 SDGTPDSKLAADGPGMFDKWLAFQLAPLASIGLPKIFSILEDLVIHTMPLPFFLVKSGYM 244

Query: 285 KLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDG 344
           KLY+AFYS S SFLAEAEKQGI+REKACHNLVFLAGFNAYGGMKVLFPILLKW+GTAG+ 
Sbjct: 245 KLYNAFYSCSDSFLAEAEKQGINREKACHNLVFLAGFNAYGGMKVLFPILLKWIGTAGEE 304

Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           LHR LADE+RAAVKEEGGLTF AIEKMSLLKSVVYEALRIEPPVPFQY KAKEDIV+ SH
Sbjct: 305 LHRNLADEIRAAVKEEGGLTFSAIEKMSLLKSVVYEALRIEPPVPFQYAKAKEDIVVQSH 364

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEEP 464
           DSAFKIKKGE IFGYQPFAT D K+FENPEKF+GDRFVG EGEKLLKYVYWSNGRETEEP
Sbjct: 365 DSAFKIKKGEIIFGYQPFATKDGKVFENPEKFIGDRFVGEEGEKLLKYVYWSNGRETEEP 424

Query: 465 TPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKAPAKV 520
           TPEDK CPGKNLVVLMARV++VEFFLRYDTFSV+V +LPLGPSVKI SVTKA  KV
Sbjct: 425 TPEDKMCPGKNLVVLMARVIVVEFFLRYDTFSVEVNDLPLGPSVKITSVTKAAVKV 480

[Close]

BLAST of Tan0000376 vs. ExPASy TrEMBL
Match: Q93XR3 (Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.3e-239
Identity = 408/473 (86.26%), Postives = 443/473 (93.66%), Query Frame = 0


Query: 44  ASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGP 103
           +SSS EL LK IPGGYGF   G IKDRYDYFY QG+DEFFRSR+TKY STVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 104 FISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHS 163
           FISSDSRV+VLLDA+SF ILFDT+K+EKRN+LDGTYMPSLSFTG+IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 164 VLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRL 223
           VLK+LFLSFLASRHDRFIPL RSSLSEMF+KLEDKL+E+KK+ADFN ISDSMSFDY+FRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 224 LSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 283
           LSDGTPDSKLAA GPG+FD WL FQLAPLASIGLPKIFSVFEDL+IHTIPLPFF VKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 284 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 343
           RKLY+AFYSSSGSFL EAEKQGIDREKACHNLVFLAGFNAYGGMKVLFP LLKWVGTAG+
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 344 GLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 403
            LHRKLA+EVR  VKEEGGLTF A+EKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI S
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 404 HDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEE 463
           HDS+FKIKKGETIFGYQPFAT DPKIF++ EKFVGDRFVG EGEKLLKYVYWSN RET E
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 464 PTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
           PTPE+KQCPGKNLVVL+ R+++VEFFLRYDTF+V+VA+LPLGP+VK KS+T+A
Sbjct: 425 PTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

[Close]

BLAST of Tan0000376 vs. ExPASy TrEMBL
Match: A0A5D3C5U2 (Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00110 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.9e-238
Identity = 407/473 (86.05%), Postives = 442/473 (93.45%), Query Frame = 0


Query: 44  ASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGP 103
           +SSS EL LK IPGGYGF   G IKDRYDYFY QG+DEFFRSR+TKY STVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 104 FISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHS 163
           FISSDSRV+VLLDA+SF ILFDT+K+EKRN+LDGTYMPSLSFTG+IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 164 VLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRL 223
           VLK+LFLSFLASRHDRFIPL RSSLSEMF+KLEDKL+E+KK+ADFN ISDSMSFDY+FRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 224 LSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 283
           LSDGTPDSKLAA GPG+FD WL FQLAPLASIGLPKIFSVFEDL+IHTIPLPFF VKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 284 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 343
           RKLY+AFYSSSGSFL EAEKQGIDREKACHNLVFLAGFNAYGGMKVLFP LLKWVGTAG+
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 344 GLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 403
            LHRKLA+EVR  VKEEGGLTF A+EKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI S
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 404 HDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEE 463
           HDS+FKIKKGETIFGYQPFAT DPKIF++ EKFVGDRFVG EGEKLLKYVYWSN RET E
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 464 PTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
           PT E+KQCPGKNLVVL+ R+++VEFFLRYDTF+V+VA+LPLGP+VK KS+T+A
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

[Close]

BLAST of Tan0000376 vs. ExPASy TrEMBL
Match: A0A1S3BZK9 (allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.9e-238
Identity = 407/473 (86.05%), Postives = 442/473 (93.45%), Query Frame = 0


Query: 44  ASSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGP 103
           +SSS EL LK IPGGYGF   G IKDRYDYFY QG+DEFFRSR+TKY STVFRANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 104 FISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHS 163
           FISSDSRV+VLLDA+SF ILFDT+K+EKRN+LDGTYMPSLSFTG+IRTCAYLDPSETEHS
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 164 VLKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRL 223
           VLK+LFLSFLASRHDRFIPL RSSLSEMF+KLEDKL+E+KK+ADFN ISDSMSFDY+FRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 224 LSDGTPDSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 283
           LSDGTPDSKLAA GPG+FD WL FQLAPLASIGLPKIFSVFEDL+IHTIPLPFF VKSGY
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 284 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 343
           RKLY+AFYSSSGSFL EAEKQGIDREKACHNLVFLAGFNAYGGMKVLFP LLKWVGTAG+
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 344 GLHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 403
            LHRKLA+EVR  VKEEGGLTF A+EKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI S
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 404 HDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETEE 463
           HDS+FKIKKGETIFGYQPFAT DPKIF++ EKFVGDRFVG EGEKLLKYVYWSN RET E
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 464 PTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
           PT E+KQCPGKNLVVL+ R+++VEFFLRYDTF+V+VA+LPLGP+VK KS+T+A
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRA 477

[Close]

BLAST of Tan0000376 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 502.7 bits (1293), Expect = 3.6e-142
Identity = 251/474 (52.95%), Postives = 331/474 (69.83%), Query Frame = 0


Query: 45  SSSSELALKSIPGGYGFSVFGAIKDRYDYFYIQGKDEFFRSRMTKYRSTVFRANMPPGPF 104
           + S +L +++IPG YG  + G IKDR+DYFY QG +EFF+SR+ KY STV+R NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 105 ISSDSRVIVLLDAVSFQILFDTSKIEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSV 164
           I+ + +V+ LLD  SF +LFD  K+EK+++  GTYMPS   TG  R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 165 LKKLFLSFLASRHDRFIPLLRSSLSEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLL 224
           LK L    L S  +R  P  +++ SE+F  LE +L+ + K ADF   SD  +F+++ R  
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 225 SDGTP-DSKLAASGPGLFDKWLTFQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGY 284
               P D+KL A  PGL  KW+ F L PL SIGLP+   V E+ +IHT  LP  LVKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 285 RKLYDAFYSSSGSFLAEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGD 344
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMK+LFP ++K +G AG 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 345 GLHRKLADEVRAAVKEEGG-LTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIH 404
            +H +LA+E+R+ +K  GG LT GAIEKM L KSVVYE LR EPPV  QYG+AK+D+VI 
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 405 SHDSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVG-EGEKLLKYVYWSNGRETE 464
           SHD+AFK+K GE ++GYQP AT DPKIF+  ++FV +RFVG EGEKLL++V WSNG ETE
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 465 EPTPEDKQCPGKNLVVLMARVLLVEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
            PT  +KQC GK+ VVL+AR+ ++E F RYD+F ++V   PLG SV   S+ KA
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

[Close]

BLAST of Tan0000376 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 266.2 bits (679), Expect = 5.7e-71
Identity = 142/391 (36.32%), Postives = 224/391 (57.29%), Query Frame = 0


Query: 129 IEKRNVLDGTYMPSLSFTGDIRTCAYLDPSETEHSVLKKLFLSFLASRHDRFIPLLRSSL 188
           ++KR+VL G + PSL F G +R   YLD +E +H+ +K   +  L      ++  LRS+L
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 189 SEMFIKLEDKLAERKKVADFNPISDSMSFDYIFRLLS--DGTPDSKLAASGPGLFDKWLT 248
           +  +  +E ++++    +   P+   + F ++   L+  D +    +A +G    + WL 
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCI-FSFLCASLAGVDASVSPDIAENGWKTINTWLA 123

Query: 249 FQLAPLASIGLPKIFSVFEDLIIHTIPLPFFLVKSGYRKLYDAFYSSSGSFLAEAEKQ-G 308
            Q+ P A +G+  +    E++++HT P P  L+   Y+KLY+    ++G  L   +++ G
Sbjct: 124 LQVIPTAKLGV--VPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 183

Query: 309 IDREKACHNLVFLAGFNAYGGMKVLFPILLKWVGTAGDGLHRKLADEVRAAVKEEGGLTF 368
           + R++A  NL+F+ GFNAYGG  V  P L+  +     GL  ++  EVR        L F
Sbjct: 184 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 243

Query: 369 GAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSHDSAFKIKKGETIFGYQPFATN 428
             + +M L+KSVVYE LR  PPVP Q+ +A++D  I SHD+ F++KKGE + GYQP    
Sbjct: 244 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 303

Query: 429 DPKIFENPEKFVGDRFVGE-GEKLLKYVYWSNGRETEEPTPEDKQCPGKNLVVLMARVLL 488
           D  +F+ PE+F  DR+VGE G +LL Y+YWSNG +T  P+  +KQC  K++V L A +L+
Sbjct: 304 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 363

Query: 489 VEFFLRYDTFSVDVANLPLGPSVKIKSVTKA 516
            + FLRYDT +        G S  IK+V KA
Sbjct: 364 ADLFLRYDTIT--------GDSGSIKAVVKA 383

[Close]

BLAST of Tan0000376 vs. TAIR 10
Match: AT5G42590.1 (cytochrome P450, family 71, subfamily A, polypeptide 16 )

HSP 1 Score: 57.8 bits (138), Expect = 3.1e-08
Identity = 42/156 (26.92%), Postives = 70/156 (44.87%), Query Frame = 0


Query: 347 RKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGK-AKEDIVIHSHD 406
           +KL DE+R+  K    ++   +E M+ LK+V+ E LR+ PP+P    +   ED+ +  +D
Sbjct: 323 KKLQDEIRSVSKMNSYVSGKEVENMNYLKAVIKEVLRLHPPLPLLVPRLLSEDVKLKGYD 382

Query: 407 ---------SAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKLLKYVYWSN 466
                    +A+ I++    +G         + F++   FVG  F        KY+ +  
Sbjct: 383 ITAGTQVIINAWAIQRDTATWGSDAQEFRPERHFDSTWDFVGRNF--------KYIPFGA 442

Query: 467 GRETEEPTPEDKQCPGKNLVVLMARVLLVEFFLRYD 493
           GR         + CPG  L  +MA V L     R+D
Sbjct: 443 GR---------RLCPGIGLGSVMASVTLANLVKRFD 461

[Close]

BLAST of Tan0000376 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 56.2 bits (134), Expect = 8.9e-08
Identity = 40/140 (28.57%), Postives = 59/140 (42.14%), Query Frame = 0


Query: 352 EVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSHDSAFKIK 411
           E+    KE+ GL +  IEKM    +V  E +RI PP+   + +A    + H     F I 
Sbjct: 319 EILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREA----IDHFSFKGFYIP 378

Query: 412 KGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKLLKYVYWSNGRETEEPTPEDKQCP 471
           KG  ++        +P  F  PE+F  +RF G G K   YV +  G          + CP
Sbjct: 379 KGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFGGG---------PRMCP 438

Query: 472 GKNLVVLMARVLLVEFFLRY 492
           GK    L   + +     R+
Sbjct: 439 GKEYARLEILIFMHNLVNRF 445

[Close]

BLAST of Tan0000376 vs. TAIR 10
Match: AT5G04630.1 (cytochrome P450, family 77, subfamily A, polypeptide 9 )

HSP 1 Score: 56.2 bits (134), Expect = 8.9e-08
Identity = 39/147 (26.53%), Postives = 67/147 (45.58%), Query Frame = 0


Query: 345 LHRKLADEVRAAVKEEGGLTFGAIEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHSH 404
           +  +L DE+++ V ++  +    + KM  L++ V E LR  PP    Y      +   ++
Sbjct: 333 IQSRLYDEIKSTVGDDRTVEEKDLNKMVFLQAFVKELLRRHPPT---YFTLTHGVTEPTN 392

Query: 405 DSAFKIKKGETIFGYQPFATNDPKIFENPEKFVGDRFVGEGEKL-------LKYVYWSNG 464
            + + I  G  +  Y P  + DPKI+  PEKF  DRF+  GE         +K + +  G
Sbjct: 393 LAGYDIPVGANVEFYLPGISEDPKIWSKPEKFDPDRFITGGEDADLTGVAGVKMMPFGIG 452

Query: 465 RETEEPTPEDKQCPGKNLVVLMARVLL 485
           R         + CPG  + V+   ++L
Sbjct: 453 R---------RICPGLGMAVVHVELML 467

The following BLAST results are available for this feature:

BLAST of Tan0000376 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GZP5	4.1e-159	57.08	Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1	[more]
K4BV52	3.5e-142	52.77	Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P...	[more]
Q96242	5.1e-141	52.95	Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P...	[more]
Q9LLB0	1.9e-140	52.77	Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P...	[more]
P48417	5.6e-140	50.95	Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE...	[more]

BLAST of Tan0000376 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988758.1	1.2e-245	89.08	allene oxide synthase 3-like [Cucurbita maxima] >XP_022988759.1 allene oxide syn...	[more]
KAG6588631.1	5.2e-244	88.52	Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG702...	[more]
XP_022931572.1	1.2e-243	88.24	allene oxide synthase 3-like [Cucurbita moschata]	[more]
XP_023530097.1	2.6e-243	88.24	allene oxide synthase 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_038886895.1	2.3e-239	86.79	allene oxide synthase 3-like [Benincasa hispida]	[more]

BLAST of Tan0000376 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JN86	6.0e-246	89.08	allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111486003 PE=4 SV...	[more]
A0A6J1EU14	5.7e-244	88.24	allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111437746 PE=4 ...	[more]
Q93XR3	9.3e-239	86.26	Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1	[more]
A0A5D3C5U2	7.9e-238	86.05	Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BZK9	7.9e-238	86.05	allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1	[more]

BLAST of Tan0000376 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42650.1	3.6e-142	52.95	allene oxide synthase	[more]
AT4G15440.1	5.7e-71	36.32	hydroperoxide lyase 1	[more]
AT5G42590.1	3.1e-08	26.92	cytochrome P450, family 71, subfamily A, polypeptide 16	[more]
AT5G36110.1	8.9e-08	28.57	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT5G04630.1	8.9e-08	26.53	cytochrome P450, family 77, subfamily A, polypeptide 9	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 424..442 score: 33.2 coord: 371..387 score: 37.78 coord: 470..488 score: 21.86
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 46..517 e-value: 1.7E-108 score: 365.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 52..497
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 347..492 e-value: 1.0E-15 score: 57.5
None	No IPR available	PANTHER	PTHR24286:SF302	HYDROPEROXIDE LYASE	coord: 50..496
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 50..496

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0000376.1	Tan0000376.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen