Tan0000219 (gene) Snake gourd v1

Overview
NameTan0000219
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionfructokinase-like 1, chloroplastic
LocationLG08: 72773274 .. 72774865 (+)
RNA-Seq ExpressionTan0000219
SyntenyTan0000219
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCATTTCAGCTCCTTCCTGCAACCTTTTCTCTTCCACATCTTCAATTTTCCCGTTTTCTTCCTTTCTACCCATCGAATTGCAGAGCTAAAACTCTGATTTTCACCTCAAATTATTTGCAGAACTCGTGTAAATCCCTGAAACTAAACCCACCAAAAGCTTCCGTAAATGGCGACAGCCCCACTCGCCGAGGGAGAAAAAAGGGGACAAAATCTCCATCTTCTTCTACAACTGCAACTCAAAAGAAAACAAAGCGCACTAAGAAAACCCTAATCGAAGACAAAACCACAGAAGGGAACCCGTTTGATCAAGTCTCAGAAGACGAAGACGAGCCAATCGATTCAATAGCAGACGATTACGACGACGGAATGGACTTCCCATACGAAGATCCACCATTGATCTGCTGCTTTGGCGCCGCACAGAAAGAATTTGTGCCCTCAATTCGTGTACACGATAACCAGATGCATCCAGATAAGTACTCGGAGTGGAAAATGCTCCAATGGGATCCGCCGGAGTTCGCTAGGGCACCGGGCGGGTCACCGTCGAATGTCGCTATATCTCACGTCAGGCTTGGCGGACGAGCTGCGTTTATGGGCAAAGTTGGGAAGGACGATTTTGGGGACGAGCTCGTGTTGATGATGAACAAAGAAAAGGTACAGACACGAGCTGTGAAGTTTGATTTGAATTCTAGAACGGCCTGTACTTACATGAAGATCAAATTTGAAGACGGAAAGATGAAGATGGAAACGGTGAAAGAACCAGCCGAGGATTCTCTGGTTAGCTCTGAACTGAACCTTGCAGTGCTCAAAGAGGTAAAATAGCCATTCACTCAAACTGGTTGTTTCATTAGAGATTTCATGAAAAGAGAAGAGAAATTGTGTTTGTTGCATTGCAGGCAAGGATTTTCCATTTCAACTCAGAAGCTTTGATGTCCACTGCAATGGAAGCAACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGGCTCATCTTCTTCGATTTAAATTTGCCGTTGCCTTTGTGGAGATCGCGCAGCGAAACTCGAGAATACATCAAGAAAGCCTGGAATGAAGCTGACATTATTGAGGTTTCAAGGCAAGAATTGGAGTTCCTGTTAGATGAAGAATACTATGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTATTGAACAAACCAAGAATCGACGAGACTATTACCATTACATGCCAGAGGAGATATCGCCCTTGTGGCACGATCGCCTCAAGTTGTTGTTCGTGACAGATGGAACGTTGCGGATTCATTACTATTCTCCTTCGTTTCATGGTGTGGTTGTCGGTACAGAAGATGTGCTCATCACACCTTTCACCTGTGACAGAACTGGTTCTGGTGATGCTGTTGTGGCTGGGATCATGAGAAAGCTGACTACTTTCCCGGAGATGTTCGAAGACCAAGATGTTTTGGAAAGGCAGATTCGATTCGCAATTGCGGCCGGGATTATTTCTCAATGGACGATTGGTGCAGTGAGAGGATTTCCTAGTGAAAGTGCAGCACAGAATCTGAAAGAGCAGGTTTATGTACCATCAATGTGGTAG

mRNA sequence

ATGGCTTCATTTCAGCTCCTTCCTGCAACCTTTTCTCTTCCACATCTTCAATTTTCCCGTTTTCTTCCTTTCTACCCATCGAATTGCAGAGCTAAAACTCTGATTTTCACCTCAAATTATTTGCAGAACTCGTGTAAATCCCTGAAACTAAACCCACCAAAAGCTTCCGTAAATGGCGACAGCCCCACTCGCCGAGGGAGAAAAAAGGGGACAAAATCTCCATCTTCTTCTACAACTGCAACTCAAAAGAAAACAAAGCGCACTAAGAAAACCCTAATCGAAGACAAAACCACAGAAGGGAACCCGTTTGATCAAGTCTCAGAAGACGAAGACGAGCCAATCGATTCAATAGCAGACGATTACGACGACGGAATGGACTTCCCATACGAAGATCCACCATTGATCTGCTGCTTTGGCGCCGCACAGAAAGAATTTGTGCCCTCAATTCGTGTACACGATAACCAGATGCATCCAGATAAGTACTCGGAGTGGAAAATGCTCCAATGGGATCCGCCGGAGTTCGCTAGGGCACCGGGCGGGTCACCGTCGAATGTCGCTATATCTCACGTCAGGCTTGGCGGACGAGCTGCGTTTATGGGCAAAGTTGGGAAGGACGATTTTGGGGACGAGCTCGTGTTGATGATGAACAAAGAAAAGGTACAGACACGAGCTGTGAAGTTTGATTTGAATTCTAGAACGGCCTGTACTTACATGAAGATCAAATTTGAAGACGGAAAGATGAAGATGGAAACGGTGAAAGAACCAGCCGAGGATTCTCTGGTTAGCTCTGAACTGAACCTTGCAGTGCTCAAAGAGGCAAGGATTTTCCATTTCAACTCAGAAGCTTTGATGTCCACTGCAATGGAAGCAACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGGCTCATCTTCTTCGATTTAAATTTGCCGTTGCCTTTGTGGAGATCGCGCAGCGAAACTCGAGAATACATCAAGAAAGCCTGGAATGAAGCTGACATTATTGAGGTTTCAAGGCAAGAATTGGAGTTCCTGTTAGATGAAGAATACTATGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTATTGAACAAACCAAGAATCGACGAGACTATTACCATTACATGCCAGAGGAGATATCGCCCTTGTGGCACGATCGCCTCAAGTTGTTGTTCGTGACAGATGGAACGTTGCGGATTCATTACTATTCTCCTTCGTTTCATGGTGTGGTTGTCGGTACAGAAGATGTGCTCATCACACCTTTCACCTGTGACAGAACTGGTTCTGGTGATGCTGTTGTGGCTGGGATCATGAGAAAGCTGACTACTTTCCCGGAGATGTTCGAAGACCAAGATGTTTTGGAAAGGCAGATTCGATTCGCAATTGCGGCCGGGATTATTTCTCAATGGACGATTGGTGCAGTGAGAGGATTTCCTAGTGAAAGTGCAGCACAGAATCTGAAAGAGCAGGTTTATGTACCATCAATGTGGTAG

Coding sequence (CDS)

ATGGCTTCATTTCAGCTCCTTCCTGCAACCTTTTCTCTTCCACATCTTCAATTTTCCCGTTTTCTTCCTTTCTACCCATCGAATTGCAGAGCTAAAACTCTGATTTTCACCTCAAATTATTTGCAGAACTCGTGTAAATCCCTGAAACTAAACCCACCAAAAGCTTCCGTAAATGGCGACAGCCCCACTCGCCGAGGGAGAAAAAAGGGGACAAAATCTCCATCTTCTTCTACAACTGCAACTCAAAAGAAAACAAAGCGCACTAAGAAAACCCTAATCGAAGACAAAACCACAGAAGGGAACCCGTTTGATCAAGTCTCAGAAGACGAAGACGAGCCAATCGATTCAATAGCAGACGATTACGACGACGGAATGGACTTCCCATACGAAGATCCACCATTGATCTGCTGCTTTGGCGCCGCACAGAAAGAATTTGTGCCCTCAATTCGTGTACACGATAACCAGATGCATCCAGATAAGTACTCGGAGTGGAAAATGCTCCAATGGGATCCGCCGGAGTTCGCTAGGGCACCGGGCGGGTCACCGTCGAATGTCGCTATATCTCACGTCAGGCTTGGCGGACGAGCTGCGTTTATGGGCAAAGTTGGGAAGGACGATTTTGGGGACGAGCTCGTGTTGATGATGAACAAAGAAAAGGTACAGACACGAGCTGTGAAGTTTGATTTGAATTCTAGAACGGCCTGTACTTACATGAAGATCAAATTTGAAGACGGAAAGATGAAGATGGAAACGGTGAAAGAACCAGCCGAGGATTCTCTGGTTAGCTCTGAACTGAACCTTGCAGTGCTCAAAGAGGCAAGGATTTTCCATTTCAACTCAGAAGCTTTGATGTCCACTGCAATGGAAGCAACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGGCTCATCTTCTTCGATTTAAATTTGCCGTTGCCTTTGTGGAGATCGCGCAGCGAAACTCGAGAATACATCAAGAAAGCCTGGAATGAAGCTGACATTATTGAGGTTTCAAGGCAAGAATTGGAGTTCCTGTTAGATGAAGAATACTATGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTATTGAACAAACCAAGAATCGACGAGACTATTACCATTACATGCCAGAGGAGATATCGCCCTTGTGGCACGATCGCCTCAAGTTGTTGTTCGTGACAGATGGAACGTTGCGGATTCATTACTATTCTCCTTCGTTTCATGGTGTGGTTGTCGGTACAGAAGATGTGCTCATCACACCTTTCACCTGTGACAGAACTGGTTCTGGTGATGCTGTTGTGGCTGGGATCATGAGAAAGCTGACTACTTTCCCGGAGATGTTCGAAGACCAAGATGTTTTGGAAAGGCAGATTCGATTCGCAATTGCGGCCGGGATTATTTCTCAATGGACGATTGGTGCAGTGAGAGGATTTCCTAGTGAAAGTGCAGCACAGAATCTGAAAGAGCAGGTTTATGTACCATCAATGTGGTAG

Protein sequence

MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGDSPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW
Homology
BLAST of Tan0000219 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 2.2e-194
Identity = 338/462 (73.16%), Postives = 386/462 (83.55%), Query Frame = 0

Query: 41  LQNSCKSLKLNPPKASVNGDSPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEG 100
           +++S  S +   PKAS+NG   T     + T  PS      + + K+   T+IE   TE 
Sbjct: 25  VRHSQASRRFLTPKASINGSGITNGAAAETTSKPS-----RKGRKKKQTSTVIEKDNTET 84

Query: 101 NPFDQVSEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDK 160
           +P           ++    DYDDG++FPY+DPPL+CCFGA QKEFVP +RVHDN MHPD 
Sbjct: 85  DP----------ELNPELADYDDGIEFPYDDPPLVCCFGAVQKEFVPVVRVHDNPMHPDM 144

Query: 161 YSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKV 220
           YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG+DDFGDELVLMMN+E+V
Sbjct: 145 YSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGEDDFGDELVLMMNQERV 204

Query: 221 QTRAVKFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNS 280
           QTRAVKFD NS+TACT +KIKF+DGKM  ETVKEP EDSL +SELNLAVLKEARIFHFNS
Sbjct: 205 QTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFASELNLAVLKEARIFHFNS 264

Query: 281 EALMSTAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVS 340
           E L S  M++TLF AIQ SKKFGGLIFFDLNLPLPLWRSR+ETR+ IKKAWNEA+IIEVS
Sbjct: 265 EVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKAWNEANIIEVS 324

Query: 341 RQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTD 400
           +QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHY PEEI  LWHD+LKLL VTD
Sbjct: 325 QQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWHDKLKLLVVTD 384

Query: 401 GTLRIHYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLE 460
           GTLR+HYY+P+F GVV+GTEDVLITPFTCDRTGSGDAVVAGIMRKLTT PEMFEDQDV+E
Sbjct: 385 GTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVME 444

Query: 461 RQIRFAIAAGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           RQ+RFA+AAGII+QWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 445 RQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Tan0000219 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 319.7 bits (818), Expect = 5.9e-86
Identity = 182/439 (41.46%), Postives = 266/439 (60.59%), Query Frame = 0

Query: 63  TRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQV---------------- 122
           TR  +K    S       T+KK +R K+T+ +DK  E +    +                
Sbjct: 120 TRTRKKAAAASSDVEEVKTEKKVRR-KRTVKKDKDVEDDLATIMDAEVSDVEEALAVEST 179

Query: 123 -SEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWK 182
            +E E+E ID    + +D +   Y  PPL+CCFG+AQ  FVPS R  +  +  + +   +
Sbjct: 180 DTESEEEEIDLSKHEGED-ISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYELHERMR 239

Query: 183 MLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 242
             +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++  +N  KVQTR+V
Sbjct: 240 DAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLYYLNVCKVQTRSV 299

Query: 243 KFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMS 302
           K D    TAC+ MKI  + G++K   +K  AEDSL  SE+N+ VLKEA++F+F++ +L+ 
Sbjct: 300 KIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEAKMFYFSTHSLLD 359

Query: 303 TAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELE 362
             M +T  +AI++SK+ G +IF+DLNLPLPLW S  ET+ +I++ WN AD+IE+++QELE
Sbjct: 360 KKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQELE 419

Query: 363 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRI 422
           FL   E  E       ++  +  + +K    + HY PE +  LWH+ LK+LFVT+GT +I
Sbjct: 420 FLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENLKVLFVTNGTSKI 479

Query: 423 HYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRF 482
           HYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++  ++  LER  R+
Sbjct: 480 HYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKGYLERTARY 539

Query: 483 AIAAGIISQWTIGAVRGFP 485
           AI  GII QW +   RG+P
Sbjct: 540 AIECGIIDQWLLAQTRGYP 544

BLAST of Tan0000219 vs. ExPASy Swiss-Prot
Match: Q42896 (Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2)

HSP 1 Score: 195.3 bits (495), Expect = 1.7e-48
Identity = 115/324 (35.49%), Postives = 176/324 (54.32%), Query Frame = 0

Query: 170 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 229
           + P F +APGG+P+NVAI+  RLGG++AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDK 92

Query: 230 NSRTACTYMKIKFEDGKMKMETVKEPAEDSLVS-SELNLAVLKEARIFHFNSEALMSTAM 289
            +RTA  ++ ++  DG+ +    + P+ D L++ +ELNL +++ A++FH+ S +L+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 290 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 349
            A   KA++++K+ G L+ +D NL LPLW S  E ++ IK  W+ AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 350 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 409
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 410 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 469
           +  FHG V G         T D TG+GD+ V  ++ K+     + ED+  L+  +RF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILEDEARLKEVLRFSCA 323

Query: 470 AGIISQWTIGAVRGFPSESAAQNL 493
            G I+    GA+   P+ S A  L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Tan0000219 vs. ExPASy Swiss-Prot
Match: Q7XJ81 (Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 6.4e-48
Identity = 115/324 (35.49%), Postives = 175/324 (54.01%), Query Frame = 0

Query: 170 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 229
           + P F +APGG+P+NVAI+  RLGGR+AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDK 92

Query: 230 NSRTACTYMKIKFEDGKMKMETVKEPAEDSLVS-SELNLAVLKEARIFHFNSEALMSTAM 289
            +RTA  ++ ++  DG+ +    + P+ D L++ +ELNL +++ A++FH+ S +L+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 290 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 349
            A   KA++++K+ G L+ +D NL LPLW S  E ++ IK  W+ AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 350 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 409
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 410 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 469
           +  FHG V G         T D TG+GD+ V  ++ K+     +  D+  L+  +RF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILADEARLKEVLRFSCA 323

Query: 470 AGIISQWTIGAVRGFPSESAAQNL 493
            G I+    GA+   P+ S A  L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Tan0000219 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.5e-46
Identity = 113/327 (34.56%), Postives = 173/327 (52.91%), Query Frame = 0

Query: 170 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 229
           D P F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ +  V    + FD 
Sbjct: 32  DAPGFIKAPGGAPANVAIAISRLGGRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDT 91

Query: 230 NSRTACTYMKIKFEDGKMKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAM 289
            +RTA  ++ ++  DG+ +    + P+ D L+   ELNL V++ A++FH+ S +L+    
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPC 151

Query: 290 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 349
            +   KA++++K+ G L+ +D NL LPLW S+ E ++ I   W++A++I+VS +EL FL 
Sbjct: 152 RSAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLT 211

Query: 350 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 409
             +  +                            E    LWH  LKLL VT G     YY
Sbjct: 212 GSDKVD---------------------------DETALSLWHSNLKLLLVTLGEKGCRYY 271

Query: 410 SPSFHGVVVGTEDVLITPF---TCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRF 469
           + SF G V         PF     D TG+GD+ V  ++ K+     + ED+  L   +R 
Sbjct: 272 TKSFRGSV--------DPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRL 322

Query: 470 AIAAGIISQWTIGAVRGFPSESAAQNL 493
           A A G I+    GA+   P+ES  Q+L
Sbjct: 332 ANACGAITTTKKGAIPALPTESEVQSL 322

BLAST of Tan0000219 vs. NCBI nr
Match: KAG7027918.1 (Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.3 bits (2294), Expect = 3.2e-254
Identity = 450/510 (88.24%), Postives = 473/510 (92.75%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFSLPHLQFSRFL FYPS  R KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKTLIFNSRYLQSSCKSLKLSPPGAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KTLIED+ T+GN FDQ+S DEDE
Sbjct: 61  GNEDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDEPTQGNLFDQMSVDEDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           F AIQLSKKFGGLIFFDLNLPLPLWRSR ETR+YI+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FNAIQLSKKFGGLIFFDLNLPLPLWRSRDETRKYIEKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL +TDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLITDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE+QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Tan0000219 vs. NCBI nr
Match: XP_023540008.1 (fructokinase-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 887.1 bits (2291), Expect = 7.1e-254
Identity = 448/510 (87.84%), Postives = 472/510 (92.55%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFSLPHLQFSRFL FYPS CR KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSICREKTLIFNSRYLQSSCKSLKLSPPSAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KT+IED+ T GN FDQ+S D+DE
Sbjct: 61  GNEDAGTPTPTRRGRKKGTKSPSSS-------TKRSRKTIIEDEPTHGNLFDQMSVDDDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           FK IQLSKKFGGLIFFDLNL LPLWRSR ETR+YI+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FKTIQLSKKFGGLIFFDLNLSLPLWRSRDETRKYIQKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL VTDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFP+MFE+QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPDMFENQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Tan0000219 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 886.7 bits (2290), Expect = 9.2e-254
Identity = 452/514 (87.94%), Postives = 476/514 (92.61%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKT----LIFTSNYLQNSCKSLKLNPPKAS 60
           MASFQLLP+T SLPH+QFS FLPFY SN RAKT    LIF S++LQ+S +SLK N P+AS
Sbjct: 1   MASFQLLPSTLSLPHVQFSCFLPFYSSNYRAKTLDLSLIFKSHHLQSSSQSLKQNTPRAS 60

Query: 61  VNGD--------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSE 120
           VNGD        SPTRRGRKKGT SPSSS  +TQKKTKR+KK  I D+TTEGNPFD  S 
Sbjct: 61  VNGDGNGVVETPSPTRRGRKKGTTSPSSS--STQKKTKRSKKPQITDETTEGNPFDHAS- 120

Query: 121 DEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 180
            EDEPIDSIAD+YDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ
Sbjct: 121 -EDEPIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 180

Query: 181 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240
           WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDE+VLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDEVVLMMNKEKVQTRAVKFD 240

Query: 241 LNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAM 300
           LNSRTACTYMKIKFEDG++KMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL+STAM
Sbjct: 241 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 300

Query: 301 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 360
           ++TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 KSTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 420
           DEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHDRLKLLFVTDGTLRIHYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRIHYY 420

Query: 421 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 480
           SPSFHGV +GTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPE+  +QD LERQ+RFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQDALERQLRFAIA 480

Query: 481 AGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           AGIISQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 510

BLAST of Tan0000219 vs. NCBI nr
Match: XP_022971326.1 (fructokinase-like 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 885.6 bits (2287), Expect = 2.1e-253
Identity = 448/510 (87.84%), Postives = 473/510 (92.75%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFS+PHLQFSRFL FYPS CR KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSIPHLQFSRFLHFYPSICREKTLIFNSRYLQSSCKSLKLSPPSAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KTLIED+ T+GN FDQ+S D+DE
Sbjct: 61  GNEDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDELTQGNLFDQMSVDDDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           FKAIQLSKKFGGLIFFDLNL LPLWRSR ETR+ I+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FKAIQLSKKFGGLIFFDLNLSLPLWRSRDETRKCIEKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL VTDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE+QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQV+VPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVFVPSMW 502

BLAST of Tan0000219 vs. NCBI nr
Match: XP_022935790.1 (fructokinase-like 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 882.1 bits (2278), Expect = 2.3e-252
Identity = 449/510 (88.04%), Postives = 471/510 (92.35%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFSLPHLQFSRFL FYPS  R KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKTLIFNSRYLQSSCKSLKLSPPGAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KTLIED+ T+ N FDQ+S DEDE
Sbjct: 61  GNEDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDEPTQVNLFDQMSVDEDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           FKAIQLSKKFGGLIFFDLNL LPLWRSR +TR+YI+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FKAIQLSKKFGGLIFFDLNLSLPLWRSRDKTRKYIEKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL VTDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFESQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Tan0000219 vs. ExPASy TrEMBL
Match: A0A6J1I311 (fructokinase-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470081 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.9e-254
Identity = 448/510 (87.84%), Postives = 473/510 (92.75%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFS+PHLQFSRFL FYPS CR KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSIPHLQFSRFLHFYPSICREKTLIFNSRYLQSSCKSLKLSPPSAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KTLIED+ T+GN FDQ+S D+DE
Sbjct: 61  GNEDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDELTQGNLFDQMSVDDDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           FKAIQLSKKFGGLIFFDLNL LPLWRSR ETR+ I+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FKAIQLSKKFGGLIFFDLNLSLPLWRSRDETRKCIEKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL VTDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE+QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQV+VPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVFVPSMW 502

BLAST of Tan0000219 vs. ExPASy TrEMBL
Match: A0A6J1FBN1 (fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595 PE=4 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.1e-252
Identity = 449/510 (88.04%), Postives = 471/510 (92.35%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKTLIFTSNYLQNSCKSLKLNPPKASVNGD 60
           MASFQLLPATFSLPHLQFSRFL FYPS  R KTLIF S YLQ+SCKSLKL+PP AS N D
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKTLIFNSRYLQSSCKSLKLSPPGAS-NAD 60

Query: 61  --------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSEDEDE 120
                   +PTRRGRKKGTKSPSSS       TKR++KTLIED+ T+ N FDQ+S DEDE
Sbjct: 61  GNEDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDEPTQVNLFDQMSVDEDE 120

Query: 121 PIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180
            IDSIA++YDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP
Sbjct: 121 AIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQWDPP 180

Query: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSR 240
           EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VKFDLNSR
Sbjct: 181 EFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVKFDLNSR 240

Query: 241 TACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAMEATL 300
           TACTYMKIKF+DGK+KMETVKE AEDSLVSSELNLAVLKEARIFHFNSEAL+ST MEATL
Sbjct: 241 TACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLSTTMEATL 300

Query: 301 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 360
           FKAIQLSKKFGGLIFFDLNL LPLWRSR +TR+YI+KAWN+ADIIEVSRQELEFLLDEEY
Sbjct: 301 FKAIQLSKKFGGLIFFDLNLSLPLWRSRDKTRKYIEKAWNQADIIEVSRQELEFLLDEEY 360

Query: 361 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 420
           YEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLL VTDGTLRIHYYSPSF
Sbjct: 361 YEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIHYYSPSF 420

Query: 421 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 480
           HGVV+GTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE QDVLERQ+RFAIAAGII
Sbjct: 421 HGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFESQDVLERQLRFAIAAGII 480

Query: 481 SQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           SQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 SQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Tan0000219 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.4e-252
Identity = 451/520 (86.73%), Postives = 474/520 (91.15%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAKT----------LIFTSNYLQNSCKSLKL 60
           MASFQLLPAT SLPHL FSRFL   PS+CR +T          LIF S Y+QNS KS +L
Sbjct: 1   MASFQLLPATISLPHLHFSRFLS-NPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRL 60

Query: 61  NPPKASVNGD--------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNP 120
           NP +ASV  D        SPTRRGRKKG    SSS+T TQKKTKR+KKTL+ED+TTEGNP
Sbjct: 61  NPARASVGDDGNGVVETASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGNP 120

Query: 121 FDQVSEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYS 180
           F+QVS  EDEPIDSIA+ YDDGMDFPYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYS
Sbjct: 121 FEQVS--EDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYS 180

Query: 181 EWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240
           EWKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT
Sbjct: 181 EWKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQT 240

Query: 241 RAVKFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEA 300
           RAVKFDLNSRTACTYMKIKFEDGK+KMETVKE AEDSL+SSELNL+VLKEARIFHFNSEA
Sbjct: 241 RAVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEA 300

Query: 301 LMSTAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQ 360
           LMSTAME+TLFKAIQLSKKFGGLIFFDLNL LPLWRSR ETREYIKKAWNEADIIEVSRQ
Sbjct: 301 LMSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQ 360

Query: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGT 420
           ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHY PEEISPLWHDRL+LLFVTDGT
Sbjct: 361 ELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGT 420

Query: 421 LRIHYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQ 480
           +RIHYYSPSFHGVV+GTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFE+QD LERQ
Sbjct: 421 MRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQ 480

Query: 481 IRFAIAAGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           +RFAIAAGIISQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 LRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of Tan0000219 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.1e-248
Identity = 443/514 (86.19%), Postives = 467/514 (90.86%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAK----TLIFTSNYLQNSCKSLKLNPPKAS 60
           MASF LLP+T  LPH+QFS FLPFY S  RAK    +LIF   + Q+S K+LKLNPP+AS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  VNGD--------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSE 120
            NG+        SPTRRGRKKGT SPSSST+A QKKTKR+KK  I DKTT  NPFD +SE
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSA-QKKTKRSKKPQITDKTTVANPFDHISE 120

Query: 121 DEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 180
           DE   IDSIAD+YDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQ
Sbjct: 121 DES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQ 180

Query: 181 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 241 LNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAM 300
           LNS+TACTYMKIKFEDGK+KMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEAL+ST +
Sbjct: 241 LNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVI 300

Query: 301 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 360
           E TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 420
           DEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLLFVTDGTLRIHYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYY 420

Query: 421 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 480
           SPSFHGV +GTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM E+QDVLERQ+RFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 481 AGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           AGIISQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Tan0000219 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.1e-248
Identity = 443/514 (86.19%), Postives = 467/514 (90.86%), Query Frame = 0

Query: 1   MASFQLLPATFSLPHLQFSRFLPFYPSNCRAK----TLIFTSNYLQNSCKSLKLNPPKAS 60
           MASF LLP+T  LPH+QFS FLPFY S  RAK    +LIF   + Q+S K+LKLNPP+AS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 61  VNGD--------SPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQVSE 120
            NG+        SPTRRGRKKGT SPSSST+A QKKTKR+KK  I DKTT  NPFD +SE
Sbjct: 61  TNGEGNGVVETPSPTRRGRKKGTASPSSSTSA-QKKTKRSKKPQITDKTTVANPFDHISE 120

Query: 121 DEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 180
           DE   IDSIAD+YDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQ
Sbjct: 121 DES--IDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQ 180

Query: 181 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 241 LNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMSTAM 300
           LNS+TACTYMKIKFEDGK+KMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEAL+ST +
Sbjct: 241 LNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVI 300

Query: 301 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 360
           E TLFKAIQLSKKFGGLIFFDLNLPLPLW+SR ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 420
           DEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHY PEEISPLWHD LKLLFVTDGTLRIHYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYY 420

Query: 421 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 480
           SPSFHGV +GTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM E+QDVLERQ+RFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 481 AGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           AGIISQWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Tan0000219 vs. TAIR 10
Match: AT3G54090.1 (fructokinase-like 1 )

HSP 1 Score: 679.9 bits (1753), Expect = 1.6e-195
Identity = 338/462 (73.16%), Postives = 386/462 (83.55%), Query Frame = 0

Query: 41  LQNSCKSLKLNPPKASVNGDSPTRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEG 100
           +++S  S +   PKAS+NG   T     + T  PS      + + K+   T+IE   TE 
Sbjct: 25  VRHSQASRRFLTPKASINGSGITNGAAAETTSKPS-----RKGRKKKQTSTVIEKDNTET 84

Query: 101 NPFDQVSEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDK 160
           +P           ++    DYDDG++FPY+DPPL+CCFGA QKEFVP +RVHDN MHPD 
Sbjct: 85  DP----------ELNPELADYDDGIEFPYDDPPLVCCFGAVQKEFVPVVRVHDNPMHPDM 144

Query: 161 YSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKV 220
           YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG+DDFGDELVLMMN+E+V
Sbjct: 145 YSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGEDDFGDELVLMMNQERV 204

Query: 221 QTRAVKFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNS 280
           QTRAVKFD NS+TACT +KIKF+DGKM  ETVKEP EDSL +SELNLAVLKEARIFHFNS
Sbjct: 205 QTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFASELNLAVLKEARIFHFNS 264

Query: 281 EALMSTAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVS 340
           E L S  M++TLF AIQ SKKFGGLIFFDLNLPLPLWRSR+ETR+ IKKAWNEA+IIEVS
Sbjct: 265 EVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKAWNEANIIEVS 324

Query: 341 RQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTD 400
           +QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRDYYHY PEEI  LWHD+LKLL VTD
Sbjct: 325 QQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWHDKLKLLVVTD 384

Query: 401 GTLRIHYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLE 460
           GTLR+HYY+P+F GVV+GTEDVLITPFTCDRTGSGDAVVAGIMRKLTT PEMFEDQDV+E
Sbjct: 385 GTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVME 444

Query: 461 RQIRFAIAAGIISQWTIGAVRGFPSESAAQNLKEQVYVPSMW 503
           RQ+RFA+AAGII+QWTIGAVRGFP+ESA QNLKEQVYVPSMW
Sbjct: 445 RQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Tan0000219 vs. TAIR 10
Match: AT1G69200.1 (fructokinase-like 2 )

HSP 1 Score: 319.7 bits (818), Expect = 4.2e-87
Identity = 182/439 (41.46%), Postives = 266/439 (60.59%), Query Frame = 0

Query: 63  TRRGRKKGTKSPSSSTTATQKKTKRTKKTLIEDKTTEGNPFDQV---------------- 122
           TR  +K    S       T+KK +R K+T+ +DK  E +    +                
Sbjct: 122 TRTRKKAAAASSDVEEVKTEKKVRR-KRTVKKDKDVEDDLATIMDAEVSDVEEALAVEST 181

Query: 123 -SEDEDEPIDSIADDYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWK 182
            +E E+E ID    + +D +   Y  PPL+CCFG+AQ  FVPS R  +  +  + +   +
Sbjct: 182 DTESEEEEIDLSKHEGED-ISHTYGWPPLVCCFGSAQHAFVPSGRPANRLLDYELHERMR 241

Query: 183 MLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAV 242
             +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++  +N  KVQTR+V
Sbjct: 242 DAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAMLYYLNVCKVQTRSV 301

Query: 243 KFDLNSRTACTYMKIKFEDGKMKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALMS 302
           K D    TAC+ MKI  + G++K   +K  AEDSL  SE+N+ VLKEA++F+F++ +L+ 
Sbjct: 302 KIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKEAKMFYFSTHSLLD 361

Query: 303 TAMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELE 362
             M +T  +AI++SK+ G +IF+DLNLPLPLW S  ET+ +I++ WN AD+IE+++QELE
Sbjct: 362 KKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQELE 421

Query: 363 FLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRI 422
           FL   E  E       ++  +  + +K    + HY PE +  LWH+ LK+LFVT+GT +I
Sbjct: 422 FLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHENLKVLFVTNGTSKI 481

Query: 423 HYYSPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRF 482
           HYY+   +G V G EDV ITPFT D + SGD +VAG++R LT  P++  ++  LER  R+
Sbjct: 482 HYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKGYLERTARY 541

Query: 483 AIAAGIISQWTIGAVRGFP 485
           AI  GII QW +   RG+P
Sbjct: 542 AIECGIIDQWLLAQTRGYP 546

BLAST of Tan0000219 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-47
Identity = 113/327 (34.56%), Postives = 173/327 (52.91%), Query Frame = 0

Query: 170 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 229
           D P F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ +  V    + FD 
Sbjct: 32  DAPGFIKAPGGAPANVAIAISRLGGRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDT 91

Query: 230 NSRTACTYMKIKFEDGKMKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAM 289
            +RTA  ++ ++  DG+ +    + P+ D L+   ELNL V++ A++FH+ S +L+    
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPC 151

Query: 290 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 349
            +   KA++++K+ G L+ +D NL LPLW S+ E ++ I   W++A++I+VS +EL FL 
Sbjct: 152 RSAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLT 211

Query: 350 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 409
             +  +                            E    LWH  LKLL VT G     YY
Sbjct: 212 GSDKVD---------------------------DETALSLWHSNLKLLLVTLGEKGCRYY 271

Query: 410 SPSFHGVVVGTEDVLITPF---TCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRF 469
           + SF G V         PF     D TG+GD+ V  ++ K+     + ED+  L   +R 
Sbjct: 272 TKSFRGSV--------DPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRL 322

Query: 470 AIAAGIISQWTIGAVRGFPSESAAQNL 493
           A A G I+    GA+   P+ES  Q+L
Sbjct: 332 ANACGAITTTKKGAIPALPTESEVQSL 322

BLAST of Tan0000219 vs. TAIR 10
Match: AT1G06030.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 186.4 bits (472), Expect = 5.5e-47
Identity = 115/325 (35.38%), Postives = 178/325 (54.77%), Query Frame = 0

Query: 174 FARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRT 233
           F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ K  V  + + FD  +RT
Sbjct: 37  FLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDEFGHMLAGILRKNDVDDQGINFDKGART 96

Query: 234 ACTYMKIKFEDGKMKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAMEATL 293
           A  ++ ++  DG+ +    + P+ D L+   ELNL +++ A++FH+ S +L++    +  
Sbjct: 97  ALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAH 156

Query: 294 FKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLLDEEY 353
            KA++++K+ G L+ +D NL  PLW S  E R+ I   W++ADII+VS  ELEFL     
Sbjct: 157 MKAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIKVSDVELEFLTGN-- 216

Query: 354 YEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYYSPSF 413
                        +TI+             E    LWH  LKLL VT G     YY+  F
Sbjct: 217 -------------KTIDD------------ETAMSLWHPNLKLLLVTLGENGCRYYTKDF 276

Query: 414 HGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIAAGII 473
           HG V   E   +     D TG+GD+ V  ++ ++     + E+++ L + +RFA A G I
Sbjct: 277 HGSV---ETFHVD--AVDTTGAGDSFVGALLNQIVDDQSVLEEEERLRKVLRFANACGAI 328

Query: 474 SQWTIGAVRGFPSESAAQN-LKEQV 497
           +    GA+   P++  A + LK QV
Sbjct: 337 TTTKKGAIPALPTDCEALSFLKIQV 328

BLAST of Tan0000219 vs. TAIR 10
Match: AT1G06020.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 186.4 bits (472), Expect = 5.5e-47
Identity = 114/328 (34.76%), Postives = 177/328 (53.96%), Query Frame = 0

Query: 170 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 229
           + P F +APGG+P+NVAI+  RLGGRAAF+GK+G DDFG  L  ++ K  V  + + FD 
Sbjct: 32  ESPGFLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDDFGHMLAGILRKNGVDDQGINFDE 91

Query: 230 NSRTACTYMKIKFEDGKMKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALMSTAM 289
            +RTA  ++ ++  DG+ +    + P+ D L+   ELNL +++ A++FH+ S +L++   
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPC 151

Query: 290 EATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRSETREYIKKAWNEADIIEVSRQELEFLL 349
            +   KA++++K+ G L+ +D NL  PLW S  E R  I   W++ADII+VS  ELEFL 
Sbjct: 152 RSAHMKAMEVAKEAGALLSYDPNLREPLWPSPEEARTQIMSIWDKADIIKVSDVELEFLT 211

Query: 350 DEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYMPEEISPLWHDRLKLLFVTDGTLRIHYY 409
           + +  + K                               LWH  LKLL VT G     Y+
Sbjct: 212 ENKTMDDK---------------------------TAMSLWHPNLKLLLVTLGEKGCTYF 271

Query: 410 SPSFHGVVVGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFEDQDVLERQIRFAIA 469
           +  FHG V   E   +     D TG+GD+ V  +++++     + ED+  L + +RFA A
Sbjct: 272 TKKFHGSV---ETFHVD--AVDTTGAGDSFVGALLQQIVDDQSVLEDEARLRKVLRFANA 326

Query: 470 AGIISQWTIGAVRGFPSE-SAAQNLKEQ 496
            G I+    GA+   P++  A   LK+Q
Sbjct: 332 CGAITTTKKGAIPALPTDIEALSFLKDQ 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3942.2e-19473.16Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 ... [more]
F4I0K25.9e-8641.46Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 ... [more]
Q428961.7e-4835.49Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2[more]
Q7XJ816.4e-4835.49Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1[more]
Q9M1B93.5e-4634.56Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG7027918.13.2e-25488.24Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023540008.17.1e-25487.84fructokinase-like 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_038906230.19.2e-25487.94fructokinase-like 1, chloroplastic [Benincasa hispida][more]
XP_022971326.12.1e-25387.84fructokinase-like 1, chloroplastic [Cucurbita maxima][more]
XP_022935790.12.3e-25288.04fructokinase-like 1, chloroplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1I3119.9e-25487.84fructokinase-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470081 P... [more]
A0A6J1FBN11.1e-25288.04fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595... [more]
A0A6J1CW731.4e-25286.73fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101513... [more]
A0A5A7TRC72.1e-24886.19Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G... [more]
A0A1S4DUM62.1e-24886.19fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G54090.11.6e-19573.16fructokinase-like 1 [more]
AT1G69200.14.2e-8741.46fructokinase-like 2 [more]
AT3G59480.12.5e-4734.56pfkB-like carbohydrate kinase family protein [more]
AT1G06030.15.5e-4735.38pfkB-like carbohydrate kinase family protein [more]
AT1G06020.15.5e-4734.76pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011611Carbohydrate kinase PfkBPFAMPF00294PfkBcoord: 162..473
e-value: 1.4E-30
score: 106.6
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 135..497
e-value: 9.1E-56
score: 191.5
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 169..487
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..103
NoneNo IPR availablePANTHERPTHR43085:SF10FRUCTOKINASE-LIKE 1, CHLOROPLASTICcoord: 87..500
NoneNo IPR availablePANTHERPTHR43085HEXOKINASE FAMILY MEMBERcoord: 87..500
NoneNo IPR availableCDDcd01167bac_FRKcoord: 134..480
e-value: 1.75974E-106
score: 317.656
IPR002173Carbohydrate/purine kinase, PfkB, conserved sitePROSITEPS00583PFKB_KINASES_1coord: 179..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000219.1Tan0000219.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0016310 phosphorylation
biological_process GO:0042793 plastid transcription
cellular_component GO:0042644 chloroplast nucleoid
molecular_function GO:0016301 kinase activity
molecular_function GO:0016773 phosphotransferase activity, alcohol group as acceptor
molecular_function GO:0043621 protein self-association