Spg039730 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg039730 vs. NCBI nr
Match: XP_038890434.1 (phytolongin Phyl2.1-like [Benincasa hispida])

HSP 1 Score: 434.5 bits (1116), Expect = 6.4e-118
Identity = 222/258 (86.05%), Postives = 235/258 (91.09%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV YACIARGT+ILAEF RESNLD LAFRCIEKAPP+HSIFSHTVR R YTFLI
Sbjct: 1   MVSNPDLVFYACIARGTIILAEFTRESNLDDLAFRCIEKAPPHHSIFSHTVRNRAYTFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDL KSE+CGFL+R RRVFE+F+ESESLK FENFT YCFQSQFDST R+
Sbjct: 61  EGPFVYFGIFHEDLNKSEICGFLNRSRRVFEEFLESESLKGFENFTSYCFQSQFDSTLRY 120

Query: 121 L-MGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGK 180
           L +GSN+NKTSS  L G QRSSLDSSKRKQIPLISKFSNPGSLMKKKKKV+GD N EGGK
Sbjct: 121 LVLGSNLNKTSSNALKGTQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVIGDVNSEGGK 180

Query: 181 YATTMDDSPDLYDDNNGLGSRDITLLMQKN-SH-VERQKAKQIWRKHVWVVLSIDLFVCL 240
           YA  MDDS DLYDDNNGL SRDITLLMQKN SH +ERQKAKQ+WRKHVWVVLSIDLFVCL
Sbjct: 181 YA--MDDSVDLYDDNNGLCSRDITLLMQKNGSHLIERQKAKQVWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFKCIES 256
           VLFAIWLWVCRGF+CIES
Sbjct: 241 VLFAIWLWVCRGFQCIES 256

BLAST of Spg039730 vs. NCBI nr
Match: XP_008458710.1 (PREDICTED: phytolongin Phyl2.1-like [Cucumis melo])

HSP 1 Score: 411.4 bits (1056), Expect = 5.8e-111
Identity = 209/257 (81.32%), Postives = 223/257 (86.77%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV Y+CIARGT+ILAEF RE NLD L  RCIEKAPP+HSIFSHT+R R YTFLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EG FVYFGIFHEDL KSEV GFL+RL+R FE F+ESESLK FENFT YCFQSQFDS+FRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKY 180
           L+G N+NKTSS  L G QRSSLDSS RKQIPL+SKFSNPGSLMKKKKKV GD N EGGKY
Sbjct: 121 LLGLNLNKTSSNGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGKY 180

Query: 181 ATTMDDSPDLYDDNNGLGSRDITLLMQKNS--HVERQKAKQIWRKHVWVVLSIDLFVCLV 240
              MDDS DLYDDNNGL SRDITLLMQKN   + ERQKAKQ+WRKHVWVVLSIDLFVCLV
Sbjct: 181 G--MDDSVDLYDDNNGLCSRDITLLMQKNGSHNSERQKAKQVWRKHVWVVLSIDLFVCLV 240

Query: 241 LFAIWLWVCRGFKCIES 256
           LF IWLWVCRGF+CIES
Sbjct: 241 LFGIWLWVCRGFQCIES 255

BLAST of Spg039730 vs. NCBI nr
Match: XP_023549951.1 (phytolongin Phyl2.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 411.0 bits (1055), Expect = 7.5e-111
Identity = 215/259 (83.01%), Postives = 227/259 (87.64%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLVLYACIA GTVILAEF RE NLD LAFRCIEKAPP+HSIFSHTVRKRVY FLI
Sbjct: 1   MVSNPDLVLYACIASGTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDLYKSE+CGFL+RLR VFE+F+E+ES K FENFT YCFQSQFDSTFRH
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLRCVFEEFLETESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLM--KKKKKVVGDANGEGG 180
           L+G N+N TS +VL   QRSSLDSSKRKQIPLISKFSNP SLM  KKKKKVVGDAN E G
Sbjct: 121 LLGLNLNNTSCSVLKEAQRSSLDSSKRKQIPLISKFSNPSSLMKKKKKKKVVGDANEEAG 180

Query: 181 KYATTMDDSPDLYDDNNGLGSRDITLLMQKN-SH-VERQKAKQIWRKHVWVVLSIDLFVC 240
           KYA  M DS DL D NNGL SRD +LL QKN SH +ERQK KQIWRKHVWVVLSIDLFVC
Sbjct: 181 KYA--MVDSADLNDHNNGLCSRDSSLLKQKNGSHMIERQKVKQIWRKHVWVVLSIDLFVC 240

Query: 241 LVLFAIWLWVCRGFKCIES 256
           LVLFAIWLWVCRGF+CIES
Sbjct: 241 LVLFAIWLWVCRGFRCIES 257

BLAST of Spg039730 vs. NCBI nr
Match: XP_022938537.1 (phytolongin Phyl2.2-like [Cucurbita moschata])

HSP 1 Score: 409.8 bits (1052), Expect = 1.7e-110
Identity = 215/258 (83.33%), Postives = 226/258 (87.60%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLVLYACIA GTVILAEF RE NLD LAFRCIEKAPP+HSIFSHTVRKRVY FLI
Sbjct: 1   MVSNPDLVLYACIASGTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDLYKSE+CGFL+RL  VFE+F+ESES K FENFT YCFQSQFDSTFRH
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLSCVFEEFLESESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLM-KKKKKVVGDANGEGGK 180
           L+G N+N TS +VL   QRSSLDSSKRKQIPLISKFSNP SLM KKKKKVVGDAN E GK
Sbjct: 121 LLGLNLNNTSYSVLKEAQRSSLDSSKRKQIPLISKFSNPSSLMKKKKKKVVGDANEEAGK 180

Query: 181 YATTMDDSPDLYDDNNGLGSRDITLLMQKN-SH-VERQKAKQIWRKHVWVVLSIDLFVCL 240
           YA  M DS DL D NNGL SRD +LL QKN SH +ERQK KQIWRKHVWVVLSIDLFVCL
Sbjct: 181 YA--MVDSADLNDHNNGLCSRDSSLLKQKNGSHMIERQKVKQIWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFKCIES 256
           VLFAIWLWVCRGF+CIES
Sbjct: 241 VLFAIWLWVCRGFRCIES 256

BLAST of Spg039730 vs. NCBI nr
Match: XP_022133479.1 (phytolongin Phyl2.2-like [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 2.2e-110
Identity = 209/258 (81.01%), Postives = 226/258 (87.60%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV YACIARGTVILAEF RESNLD LAFRCIEKAPPNHS FSHTVR R Y FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           E  FVYFGIFHEDLYKSEV GFL+R++RVF DFIESE L  F+NFTPYCFQ+QFDS FR 
Sbjct: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120

Query: 121 LMGSNMNKTSS-TVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGK 180
           ++GSN+N + + +  +G  R S+DSS RKQIP ISKFSNP +LMKKKKKVVGDANGEGGK
Sbjct: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180

Query: 181 YATTMDDSPDLYDDNNGLGSRDITLLMQKN-SHV-ERQKAKQIWRKHVWVVLSIDLFVCL 240
           YATTMDDS D YDD NGLGSRDITLLMQKN SH+ ERQKAKQIW+KHVWVVLSIDLFVCL
Sbjct: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFKCIES 256
           VLFAIWLWVCRGF+CIES
Sbjct: 241 VLFAIWLWVCRGFQCIES 258

BLAST of Spg039730 vs. ExPASy Swiss-Prot
Match: Q9LVV3 (Phytolongin Phyl2.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.2 PE=2 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.6e-45
Identity = 108/272 (39.71%), Postives = 156/272 (57.35%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFA--RESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTF 60
           M+SNP L+ Y CIA+GTV+LAEF   +E  ++ +A RCIE  PP+HS+FSHTV K+ YTF
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIE--SESLKDFENFTPYCFQSQFD 120
            I +  FVYF I  E + K E    L+RLR   ED I+     ++   N   +C Q + D
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKDGGSDVETLINPVSHCLQLKLD 120

Query: 121 STFRHLMG--------SNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKK 180
             F  ++G         ++  +  +V    +  S+DSSK ++  L+     P   +KKKK
Sbjct: 121 PVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALKKKK 180

Query: 181 KVVGDANG----EGGKYATTMDDSPDLY-DDNNGLGSRDITLLMQKNSHVERQKAKQIWR 240
           ++  +A G    E G      + + DL  + NNG+  +++   +  + H  RQKAKQIW+
Sbjct: 181 RLHNEAKGGDSCEVGSIQEISEKNVDLCGNGNNGVLRKELRNGLLSDHH-HRQKAKQIWK 240

Query: 241 KHVWVVLSIDLFVCLVLFAIWLWVCRGFKCIE 255
           KHVWVVL  D  +C VLF IWLW+C GF+CI+
Sbjct: 241 KHVWVVLMFDFCICAVLFGIWLWICEGFQCIQ 271

BLAST of Spg039730 vs. ExPASy Swiss-Prot
Match: Q9STP2 (Phytolongin Phyl2.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL2.1 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 4.4e-45
Identity = 117/270 (43.33%), Postives = 149/270 (55.19%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFA--RESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTF 60
           M SN  ++ Y+CIA+GTVILAEFA   E  ++ LA RCIE  PP+HS+ SHTV KR Y  
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKD-FENFTPYCFQSQFDST 120
           +I+G F YF I  E + KSE     +RL+   E  +E  S  D  +N T +C QS+ D  
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRHL--MGSNMNKTSSTVLNGGQRS----------SLDSSK-RKQIPLISKFSNPGSLMK 180
           F  +  +G N NK       G  RS          SLDSSK RK   L+     P  ++K
Sbjct: 121 FAEIAAIGGNHNKDLELEF-GSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLK 180

Query: 181 KKKKVVGDANGEGGKYATTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKH 240
            KK++  +A  EG      MD         NGL           + H  RQKAKQIWRKH
Sbjct: 181 NKKRLQTEAKSEGHWNEKKMDLGGGGKGVRNGL----------IHDHHHRQKAKQIWRKH 240

Query: 241 VWVVLSIDLFVCLVLFAIWLWVCRGFKCIE 255
           VW+VL  DL +CLVLF IWLW+C+GF+CI+
Sbjct: 241 VWIVLMFDLCICLVLFGIWLWICQGFQCID 259

BLAST of Spg039730 vs. ExPASy Swiss-Prot
Match: Q9SN26 (Phytolongin Phyl1.1 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.3e-17
Identity = 78/254 (30.71%), Postives = 118/254 (46.46%), Query Frame = 0

Query: 8   VLYACIARGTVILAEF-ARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLIEGPFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+ KR + FLI   FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFT-PYCFQSQFDSTFRHLMGSN 127
           F I  E L +S V  FL  LR  F+      S   F         + Q       L+ S 
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLRDEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLIASL 127

Query: 128 MNKTSSTVLNGGQRSSL----DSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKYA 187
                S+  N  + S+L    + S   + PL+      G L K++KK   D   E  ++ 
Sbjct: 128 ERVAESSSNNELKSSNLGEQSEGSNSTKAPLL------GRLSKQEKKKGKDHVIELEEHR 187

Query: 188 TTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSIDLFVCLVLFA 247
            + +D  ++ DD+ G G+      +  +     Q  +  WR+ V +VL+ID  +CL LF 
Sbjct: 188 KS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWKWRRLVQIVLAIDAAICLTLFG 247

Query: 248 IWLWVCRGFKCIES 256
           IWL +CRG +C  S
Sbjct: 248 IWLAICRGIECTRS 254

BLAST of Spg039730 vs. ExPASy Swiss-Prot
Match: Q84WF5 (Phytolongin Phyl1.2 OS=Arabidopsis thaliana OX=3702 GN=PHYL1.2 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.1e-15
Identity = 71/263 (27.00%), Postives = 118/263 (44.87%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEF---ARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYT 60
           M S  + V Y C++R   I+  +       N + LA  C+EK PP H  +  T  K+ + 
Sbjct: 1   MGSIQNTVHYCCVSRDNQIMYAYNNAGDHRNNESLAALCLEKTPPFHKWYFETRGKKTFG 60

Query: 61  FLIEGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDST 120
           FL++  FVYF I  +   KS V  FL +LR   ++  +  S   F       F +  D  
Sbjct: 61  FLMKDDFVYFAIVDDVFKKSSVLDFLEKLRDELKEANKKNSRGSFSG--SISFSNVQDQI 120

Query: 121 FRHLMGS-----NMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGD 180
            R L+ S          SS  ++G ++S   +SK    PL+ + +      KK +     
Sbjct: 121 VRRLIASLEFDHTCLPLSSPSIDGAEQSYASNSK---APLLGRSNKQDK--KKGRDHAHS 180

Query: 181 ANGEGGKYATTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSID 240
             G   +     +D  ++ + +N   S   T + ++      Q  ++ WR+ V +VL+ID
Sbjct: 181 LRGIEIEEHRKSNDRGNVTECSNA-SSESATYVPRRGRSGGSQSIERKWRRQVKIVLAID 240

Query: 241 LFVCLVLFAIWLWVCRGFKCIES 256
           + +CL L  +WL +C G +C  S
Sbjct: 241 IAICLTLLGVWLAICHGIECTRS 255

BLAST of Spg039730 vs. ExPASy Swiss-Prot
Match: Q9M376 (Vesicle-associated membrane protein 727 OS=Arabidopsis thaliana OX=3702 GN=VAMP727 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.6e-07
Identity = 58/258 (22.48%), Postives = 113/258 (43.80%), Query Frame = 0

Query: 8   VLYACIARGTVILAEFARES-NLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLIEGPFVY 67
           ++Y+ +A+GTV+LAE    S N   +A +C++K P N S ++++     + FL++  FV+
Sbjct: 6   LIYSFVAKGTVVLAEHTPYSGNFSTIAVQCLQKLPTNSSKYTYSCDGHTFNFLVDNGFVF 65

Query: 68  FGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTF--RHLMGS 127
             +  E   +S    FL R++  F+   E+ S+K+ E   P   + + D  F  R  +  
Sbjct: 66  LVVADESTGRSVPFVFLERVKEDFKKRYEA-SIKNDERH-PLADEDEDDDLFGDRFSVAY 125

Query: 128 NMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKYATTM 187
           N+++    +L            ++ +          S + K K  + +  G        M
Sbjct: 126 NLDREFGPIL------------KEHMQYCMSHPEEMSKLSKLKAQITEVKG------IMM 185

Query: 188 DDSPDLYDDNNGLGSRDITLLMQKNSHVE----------RQKAKQIWRKHVWVVLSIDLF 247
           D+   + D         I LL+ K  +++          RQ  +++W + + + L +   
Sbjct: 186 DNIEKVLD-----RGEKIELLVDKTENLQFQADSFQRQGRQLRRKMWLQSLQMKLMVAGA 238

Query: 248 VCLVLFAIWLWVCRGFKC 253
           V   +  +W+  C GFKC
Sbjct: 246 VFSFILIVWVVACGGFKC 238

BLAST of Spg039730 vs. ExPASy TrEMBL
Match: A0A1S3C809 (phytolongin Phyl2.1-like OS=Cucumis melo OX=3656 GN=LOC103498036 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.8e-111
Identity = 209/257 (81.32%), Postives = 223/257 (86.77%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV Y+CIARGT+ILAEF RE NLD L  RCIEKAPP+HSIFSHT+R R YTFLI
Sbjct: 1   MVSNPDLVFYSCIARGTIILAEFTREPNLDDLPLRCIEKAPPHHSIFSHTIRNRAYTFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EG FVYFGIFHEDL KSEV GFL+RL+R FE F+ESESLK FENFT YCFQSQFDS+FRH
Sbjct: 61  EGSFVYFGIFHEDLNKSEVSGFLNRLKRDFEVFLESESLKGFENFTSYCFQSQFDSSFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKY 180
           L+G N+NKTSS  L G QRSSLDSS RKQIPL+SKFSNPGSLMKKKKKV GD N EGGKY
Sbjct: 121 LLGLNLNKTSSNGLMGSQRSSLDSSTRKQIPLVSKFSNPGSLMKKKKKVTGDGNWEGGKY 180

Query: 181 ATTMDDSPDLYDDNNGLGSRDITLLMQKNS--HVERQKAKQIWRKHVWVVLSIDLFVCLV 240
              MDDS DLYDDNNGL SRDITLLMQKN   + ERQKAKQ+WRKHVWVVLSIDLFVCLV
Sbjct: 181 G--MDDSVDLYDDNNGLCSRDITLLMQKNGSHNSERQKAKQVWRKHVWVVLSIDLFVCLV 240

Query: 241 LFAIWLWVCRGFKCIES 256
           LF IWLWVCRGF+CIES
Sbjct: 241 LFGIWLWVCRGFQCIES 255

BLAST of Spg039730 vs. ExPASy TrEMBL
Match: A0A6J1FEB5 (phytolongin Phyl2.2-like OS=Cucurbita moschata OX=3662 GN=LOC111444745 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 8.1e-111
Identity = 215/258 (83.33%), Postives = 226/258 (87.60%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLVLYACIA GTVILAEF RE NLD LAFRCIEKAPP+HSIFSHTVRKRVY FLI
Sbjct: 1   MVSNPDLVLYACIASGTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDLYKSE+CGFL+RL  VFE+F+ESES K FENFT YCFQSQFDSTFRH
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLSCVFEEFLESESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLM-KKKKKVVGDANGEGGK 180
           L+G N+N TS +VL   QRSSLDSSKRKQIPLISKFSNP SLM KKKKKVVGDAN E GK
Sbjct: 121 LLGLNLNNTSYSVLKEAQRSSLDSSKRKQIPLISKFSNPSSLMKKKKKKVVGDANEEAGK 180

Query: 181 YATTMDDSPDLYDDNNGLGSRDITLLMQKN-SH-VERQKAKQIWRKHVWVVLSIDLFVCL 240
           YA  M DS DL D NNGL SRD +LL QKN SH +ERQK KQIWRKHVWVVLSIDLFVCL
Sbjct: 181 YA--MVDSADLNDHNNGLCSRDSSLLKQKNGSHMIERQKVKQIWRKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFKCIES 256
           VLFAIWLWVCRGF+CIES
Sbjct: 241 VLFAIWLWVCRGFRCIES 256

BLAST of Spg039730 vs. ExPASy TrEMBL
Match: A0A6J1BW35 (phytolongin Phyl2.2-like OS=Momordica charantia OX=3673 GN=LOC111006049 PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 209/258 (81.01%), Postives = 226/258 (87.60%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV YACIARGTVILAEF RESNLD LAFRCIEKAPPNHS FSHTVR R Y FLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFTRESNLDDLAFRCIEKAPPNHSTFSHTVRNRAYAFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           E  FVYFGIFHEDLYKSEV GFL+R++RVF DFIESE L  F+NFTPYCFQ+QFDS FR 
Sbjct: 61  EESFVYFGIFHEDLYKSEVSGFLNRVKRVFTDFIESELLNGFDNFTPYCFQAQFDSIFRQ 120

Query: 121 LMGSNMNKTSS-TVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGK 180
           ++GSN+N + + +  +G  R S+DSS RKQIP ISKFSNP +LMKKKKKVVGDANGEGGK
Sbjct: 121 ILGSNLNTSGNLSTSSGVLRPSVDSSIRKQIPFISKFSNPSNLMKKKKKVVGDANGEGGK 180

Query: 181 YATTMDDSPDLYDDNNGLGSRDITLLMQKN-SHV-ERQKAKQIWRKHVWVVLSIDLFVCL 240
           YATTMDDS D YDD NGLGSRDITLLMQKN SH+ ERQKAKQIW+KHVWVVLSIDLFVCL
Sbjct: 181 YATTMDDSADPYDDMNGLGSRDITLLMQKNGSHIAERQKAKQIWQKHVWVVLSIDLFVCL 240

Query: 241 VLFAIWLWVCRGFKCIES 256
           VLFAIWLWVCRGF+CIES
Sbjct: 241 VLFAIWLWVCRGFQCIES 258

BLAST of Spg039730 vs. ExPASy TrEMBL
Match: A0A6J1JWY4 (phytolongin Phyl2.2-like OS=Cucurbita maxima OX=3661 GN=LOC111489585 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 213/259 (82.24%), Postives = 225/259 (86.87%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLVLYACIA  TVILAEF RE NLD LAFRCIEKAPP+HSIFSHTVRKRVY FLI
Sbjct: 1   MVSNPDLVLYACIASRTVILAEFTRELNLDDLAFRCIEKAPPHHSIFSHTVRKRVYMFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDLYKSE+CGFL+RLR VFE+F+E+ES K FENFT YCFQSQFDSTFRH
Sbjct: 61  EGPFVYFGIFHEDLYKSEICGFLNRLRCVFEEFLETESFKGFENFTSYCFQSQFDSTFRH 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLM--KKKKKVVGDANGEGG 180
           L+G N+N TS +V    QRSSLDSSKRKQIPLISKFSNP SLM  KKKKKVVGDAN E G
Sbjct: 121 LLGLNLNNTSYSVSKEAQRSSLDSSKRKQIPLISKFSNPSSLMKKKKKKKVVGDANEEAG 180

Query: 181 KYATTMDDSPDLYDDNNGLGSRDITLLMQKN-SH-VERQKAKQIWRKHVWVVLSIDLFVC 240
           KYA  M DS DL D NNGL SRD +LL QKN SH +ERQK KQIWRKHVWVVLSIDLFVC
Sbjct: 181 KYA--MVDSADLNDHNNGLCSRDSSLLKQKNGSHMIERQKVKQIWRKHVWVVLSIDLFVC 240

Query: 241 LVLFAIWLWVCRGFKCIES 256
           LVLFAIWLWVCRGF+CIES
Sbjct: 241 LVLFAIWLWVCRGFRCIES 257

BLAST of Spg039730 vs. ExPASy TrEMBL
Match: A0A6J1JQM5 (phytolongin Phyl2.1-like OS=Cucurbita maxima OX=3661 GN=LOC111487464 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 9.3e-107
Identity = 203/255 (79.61%), Postives = 216/255 (84.71%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLI 60
           MVSNPDLV YACIARGTVILAEF RE NL+ LAFRCIEKAPP+HS FSHTVR RVYTFLI
Sbjct: 1   MVSNPDLVFYACIARGTVILAEFNREPNLNDLAFRCIEKAPPHHSTFSHTVRNRVYTFLI 60

Query: 61  EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDSTFRH 120
           EGPFVYFGIFHEDL+KSEVCGFLSRLRR FE+FIESE  K FENFT  CFQSQFDS FR 
Sbjct: 61  EGPFVYFGIFHEDLFKSEVCGFLSRLRRAFEEFIESELRKGFENFTSNCFQSQFDSIFRR 120

Query: 121 LMGSNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKY 180
           ++ SN ++TS         S LDS KRK+  LISKFS P SLMKKKKKVVGDAN E GKY
Sbjct: 121 ILTSNQDQTS---------SGLDSDKRKKTSLISKFSKPCSLMKKKKKVVGDANSEEGKY 180

Query: 181 ATTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSIDLFVCLVLF 240
           A+T+DDS DLYDDNNGL SRD+TLLMQKN   ERQKAKQIWRKHVWVVLSIDLFVCLVLF
Sbjct: 181 ASTVDDSVDLYDDNNGLCSRDVTLLMQKNGSYERQKAKQIWRKHVWVVLSIDLFVCLVLF 240

Query: 241 AIWLWVCRGFKCIES 256
           AIWLWVC GF+CIES
Sbjct: 241 AIWLWVCSGFQCIES 246

BLAST of Spg039730 vs. TAIR 10
Match: AT5G52990.1 (SNARE-like superfamily protein )

HSP 1 Score: 183.7 bits (465), Expect = 1.8e-46
Identity = 108/272 (39.71%), Postives = 156/272 (57.35%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFA--RESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTF 60
           M+SNP L+ Y CIA+GTV+LAEF   +E  ++ +A RCIE  PP+HS+FSHTV K+ YTF
Sbjct: 1   MISNPSLLSYTCIAKGTVVLAEFVSRQEPGIEAIALRCIENTPPHHSMFSHTVHKKTYTF 60

Query: 61  LI-EGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIE--SESLKDFENFTPYCFQSQFD 120
            I +  FVYF I  E + K E    L+RLR   ED I+     ++   N   +C Q + D
Sbjct: 61  AIDDDSFVYFSISDESMEKPESFWVLNRLRSAIEDLIKDGGSDVETLINPVSHCLQLKLD 120

Query: 121 STFRHLMG--------SNMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKK 180
             F  ++G         ++  +  +V    +  S+DSSK ++  L+     P   +KKKK
Sbjct: 121 PVFAEIVGVVDLELLDMDLVGSPRSVARESRNPSIDSSKGRRAALMPLLGKPLKALKKKK 180

Query: 181 KVVGDANG----EGGKYATTMDDSPDLY-DDNNGLGSRDITLLMQKNSHVERQKAKQIWR 240
           ++  +A G    E G      + + DL  + NNG+  +++   +  + H  RQKAKQIW+
Sbjct: 181 RLHNEAKGGDSCEVGSIQEISEKNVDLCGNGNNGVLRKELRNGLLSDHH-HRQKAKQIWK 240

Query: 241 KHVWVVLSIDLFVCLVLFAIWLWVCRGFKCIE 255
           KHVWVVL  D  +C VLF IWLW+C GF+CI+
Sbjct: 241 KHVWVVLMFDFCICAVLFGIWLWICEGFQCIQ 271

BLAST of Spg039730 vs. TAIR 10
Match: AT4G27840.1 (SNARE-like superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 3.1e-46
Identity = 117/270 (43.33%), Postives = 149/270 (55.19%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEFA--RESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTF 60
           M SN  ++ Y+CIA+GTVILAEFA   E  ++ LA RCIE  PP+HS+ SHTV KR Y  
Sbjct: 1   MTSNQRMLFYSCIAKGTVILAEFASKEEPGIEDLALRCIENVPPHHSMISHTVHKRTYAL 60

Query: 61  LIEGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKD-FENFTPYCFQSQFDST 120
           +I+G F YF I  E + KSE     +RL+   E  +E  S  D  +N T +C QS+ D  
Sbjct: 61  IIDGLFSYFAILDEVVAKSESVWLFNRLKSATESLMEDGSTADSLDNPTQHCLQSKLDPV 120

Query: 121 FRHL--MGSNMNKTSSTVLNGGQRS----------SLDSSK-RKQIPLISKFSNPGSLMK 180
           F  +  +G N NK       G  RS          SLDSSK RK   L+     P  ++K
Sbjct: 121 FAEIAAIGGNHNKDLELEF-GSPRSIAREIKSNNQSLDSSKGRKGGALMPLLGKPLRVLK 180

Query: 181 KKKKVVGDANGEGGKYATTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKH 240
            KK++  +A  EG      MD         NGL           + H  RQKAKQIWRKH
Sbjct: 181 NKKRLQTEAKSEGHWNEKKMDLGGGGKGVRNGL----------IHDHHHRQKAKQIWRKH 240

Query: 241 VWVVLSIDLFVCLVLFAIWLWVCRGFKCIE 255
           VW+VL  DL +CLVLF IWLW+C+GF+CI+
Sbjct: 241 VWIVLMFDLCICLVLFGIWLWICQGFQCID 259

BLAST of Spg039730 vs. TAIR 10
Match: AT4G10170.1 (SNARE-like superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-18
Identity = 78/254 (30.71%), Postives = 118/254 (46.46%), Query Frame = 0

Query: 8   VLYACIARGTVILAEF-ARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLIEGPFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+ KR + FLI   FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFT-PYCFQSQFDSTFRHLMGSN 127
           F I  E L +S V  FL  LR  F+      S   F         + Q       L+ S 
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLRDEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLIASL 127

Query: 128 MNKTSSTVLNGGQRSSL----DSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKYA 187
                S+  N  + S+L    + S   + PL+      G L K++KK   D   E  ++ 
Sbjct: 128 ERVAESSSNNELKSSNLGEQSEGSNSTKAPLL------GRLSKQEKKKGKDHVIELEEHR 187

Query: 188 TTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSIDLFVCLVLFA 247
            + +D  ++ DD+ G G+      +  +     Q  +  WR+ V +VL+ID  +CL LF 
Sbjct: 188 KS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWKWRRLVQIVLAIDAAICLTLFG 247

Query: 248 IWLWVCRGFKCIES 256
           IWL +CRG +C  S
Sbjct: 248 IWLAICRGIECTRS 254

BLAST of Spg039730 vs. TAIR 10
Match: AT4G10170.2 (SNARE-like superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-18
Identity = 78/254 (30.71%), Postives = 118/254 (46.46%), Query Frame = 0

Query: 8   VLYACIARGTVILAEF-ARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYTFLIEGPFVY 67
           V Y C++R   IL  +   +   + LA  C+EK+PP H+ +  T+ KR + FLI   FVY
Sbjct: 8   VHYCCVSRDNQILYSYNGGDQTNESLAALCLEKSPPFHTWYFETIGKRRFGFLIGDGFVY 67

Query: 68  FGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFT-PYCFQSQFDSTFRHLMGSN 127
           F I  E L +S V  FL  LR  F+      S   F         + Q       L+ S 
Sbjct: 68  FAIVDEVLKRSSVLKFLEHLRDEFKKAARENSRGSFTAMIGSINVEDQLVPVVTRLIASL 127

Query: 128 MNKTSSTVLNGGQRSSL----DSSKRKQIPLISKFSNPGSLMKKKKKVVGDANGEGGKYA 187
                S+  N  + S+L    + S   + PL+      G L K++KK   D   E  ++ 
Sbjct: 128 ERVAESSSNNELKSSNLGEQSEGSNSTKAPLL------GRLSKQEKKKGKDHVIELEEHR 187

Query: 188 TTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSIDLFVCLVLFA 247
            + +D  ++ DD+ G G+      +  +     Q  +  WR+ V +VL+ID  +CL LF 
Sbjct: 188 KS-NDRGNITDDSAGAGTSLEKECVSSSGRSVTQSFEWKWRRLVQIVLAIDAAICLTLFG 247

Query: 248 IWLWVCRGFKCIES 256
           IWL +CRG +C  S
Sbjct: 248 IWLAICRGIECTRS 254

BLAST of Spg039730 vs. TAIR 10
Match: AT1G33475.1 (SNARE-like superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-16
Identity = 71/263 (27.00%), Postives = 118/263 (44.87%), Query Frame = 0

Query: 1   MVSNPDLVLYACIARGTVILAEF---ARESNLDVLAFRCIEKAPPNHSIFSHTVRKRVYT 60
           M S  + V Y C++R   I+  +       N + LA  C+EK PP H  +  T  K+ + 
Sbjct: 1   MGSIQNTVHYCCVSRDNQIMYAYNNAGDHRNNESLAALCLEKTPPFHKWYFETRGKKTFG 60

Query: 61  FLIEGPFVYFGIFHEDLYKSEVCGFLSRLRRVFEDFIESESLKDFENFTPYCFQSQFDST 120
           FL++  FVYF I  +   KS V  FL +LR   ++  +  S   F       F +  D  
Sbjct: 61  FLMKDDFVYFAIVDDVFKKSSVLDFLEKLRDELKEANKKNSRGSFSG--SISFSNVQDQI 120

Query: 121 FRHLMGS-----NMNKTSSTVLNGGQRSSLDSSKRKQIPLISKFSNPGSLMKKKKKVVGD 180
            R L+ S          SS  ++G ++S   +SK    PL+ + +      KK +     
Sbjct: 121 VRRLIASLEFDHTCLPLSSPSIDGAEQSYASNSK---APLLGRSNKQDK--KKGRDHAHS 180

Query: 181 ANGEGGKYATTMDDSPDLYDDNNGLGSRDITLLMQKNSHVERQKAKQIWRKHVWVVLSID 240
             G   +     +D  ++ + +N   S   T + ++      Q  ++ WR+ V +VL+ID
Sbjct: 181 LRGIEIEEHRKSNDRGNVTECSNA-SSESATYVPRRGRSGGSQSIERKWRRQVKIVLAID 240

Query: 241 LFVCLVLFAIWLWVCRGFKCIES 256
           + +CL L  +WL +C G +C  S
Sbjct: 241 IAICLTLLGVWLAICHGIECTRS 255