Spg039685 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039685
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPlasma membrane intrinsic protein
Locationscaffold10: 42030570 .. 42034985 (+)
RNA-Seq ExpressionSpg039685
SyntenySpg039685
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGCCGTAAGAGTAAAGATGCAAATGCTATCACTTCTAAGCTACGTCCTTCTATTAAACTGTAAATACATACACAGGGGAAAGGAGTTCAAAAGGTTACCGCCATTGATTATTTATTTGAAGCATGAAAAGGAATTTGTCGTTACTGTAGTAAGTGGTATGCACAGTGAAACCACTGTTCTGAGTGGGTCAGAGCGACAAAGGTACGGAGCAGAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCAGAAAATCACTGCTCCTCTTCTCTTCTTTGCCTTCAATTTCCTCTCATTAAATGCCGCTCTTCGCTCCCCAACTTCGCACCATATTCATTCACAAATCCTCGCCGGAAATTGCTTCTCTCTCCTAGGGTTTCGATTTCTCTGTCTGTCTTTATGGCGAAGAATGCGGCGGAGCGCGGCTCGTTCTCGGCGAAGGACTACCAGGATCCGCCACCGGCGCCGCTCATCGACGCCGTGGAGATTACGAAGTGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTTTGCTTTTCCTTTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAATGTGGATCCTTGCGGTGGCGTTGGGATTCTGGGAATTGCTTGGGCATTTGGAGGAATGATCTTCGTTCTTGTTTACTGTACTGCTGGAATCTCAGGTTCTTCTTCCTCTCTTCCTTCAGCTTAAAGTTGAAGCGTTGAACTTGAACTTCTTTCTTCCATTTTCTTTCATTTTCTCGGCTTCCAAACATAACATATTGCTACGTTGCGGCTATAATATTATCATGCAGTTATTTTATTATCATTATTTTGAAATAATGCACAATTTTTTTTTTTTTTTTTTTTTGGAACAGAATTTGAAATAATGTACTTATTATATCTTTTCTTTTATTACATTTTCGTCGTGTAAATTTGTAATATGTTTCAAAAATTTTTTTTAAAAAAATCGTCTTGTAATTTATGACTTTATTTCATTAAAAAGAAAATTATGATTTTATTTCCATTTGGCGCTTAATCCTTTTTTTCTTTATTTCCATAATTTTATTATTTTTTATCTCCCTATCCGTTGAAATAGTTGCCTTCAGAAGTTCGTCAAAAAAAAAAAAAAAAAAAAAAAGTTGCCTTAAGAACTGGTAGATAAATATTAATATATAACTTTTTCTAGTACATATTAATTTATAATTCTTATAGTACATGTTAACTTATAATTTATAATATTATTACTATTAAAAAATAAACATCTGCAAAATGATGCAAATTTGCACTCCGCTTATCATATCAACACATATATGGTGAGCTATAGATTTAATATTATTATTTTCAAAACGCTATTTATAAATTAATAGGTTGAGTCAAAGCGAGCATGACTTAGCGGTAATTGGCATTTATTCTCTGACCTGAGTATTTATCATCGACCAAGAGGTCCGAGGTTCGAATTTCCCCACCCTAAATGTTGACATATTCAGAAAATAATAATAATAATAATAATAGGTTGAACTATACTAAGTCAAATGAATCACAAATTTGAATAAAAAAACGCGGAAGTAAATATCCCTTTTTTTTAGCTTGGATGTCTTAATTAGTTAATCTGTGTTTATCAATCTCAACTCTACTCTTTATGTATAAACCTAATAATAATAGTATTTATGTACTGTATAATAAAACTAAACCAAAATGATAGATGAATCAGATTTATGTAGTCGATCAATTTACTCCTTCGCATGTAATATTTAACTTTTCTTTTTGACAATATGTGAAATGGAGAGATTCGAACTACAAACTTCTTGTTTATTAGTACAAGTTTTATGTCAGTTAAGCTGTGGTCATAAAGCTAAGGTTAATCATTGGTCGTCGCCAACCTAAATTCTCATTTCTACTCCTTTATATTTCATATTTTTATCTGTGATGTTGCATTACTATTATTATTATTAAAAATTATATATATATAAAGAAAAGAAAAGAAAAAGCTAAACAGTTGAGAATGTGCAGGGGGCCACATAAACCCAGCAGTGACATTTGGGCTGCTGTTGGCCCGTAAAGTGTCACTGGTGCGGGCCGTGATGTACATGGTGGCCCAATGCTTGGGGGCCATAAGCGGAGTTGGGCTCGTAAAGGCCTTCCAGAAGGCCCACTTCCAGAAGCACGGCGGCGGGGCCAATGGGCTCGCCGACGGCTACACTGTCGGCGTCGGGCTGGCCGCCGAGATTGTCGGCACGTTTGTGTTGGTGTACACAGTCTTTTCTGCTACTGATCCCAAACGAAACGCCAGAGATTCTCATGTTCCTGTATGCATATCTTAACCCTTCTCTCTCTCTCTTTTAAATTTTAATTTTATAAATGATTTTTCAAAAATTCATATATTAAACTATTAGAATCCTCTCAAGATATGTCTACAAAATTAAATCAATGTAATTCAAATCATAAAATTATAATTTTAATTCTCTACACATTACACCTTAGATAAATAAGGTTGTTGCCAACGTGAGTATCGTCATTTATTTATTTCTTGAACGTTGTAGATTCAAACACTCACCCCATATTTCCTAAAAAAAAAAAAAAAAAAACACTCACCCCATATTTGTAGGTTTCAAATTTCCATCCGTGAATGATACAACATTCTCAAATAACTAGAAAGAAACTATTAAGATATTTTTACTTCTAAAAAACGTTGAGGCCTTACCCTTGAAAAAAAATGATATTTTTTTTTTCTCTTTCCTAGTTGTCGACATTTTAACAAAAACAAAAAAACAAAAATAGTGGATTTTACTTGGCAAGTAGTGTTAGGTAGTGGATTTGAGAATCACCCAACTTTTTGTTTGTTTCCTAGGAAAACTGGATTCCTTCTGGGAAAATCTTTTCCTCATCTACATGTCATAGTGGTTTTCCAAGAGTTCTAATTCTAAATAGTCACAAAAGATGACCTTTTCTTTTTCTCTTTAACCAAGCTAGCAAAATATTATTTTTAATGGTCTCTTAATTTTCTTTTTATTGAAAGAATTACAAAGTGGGTTCATGTGTTAGTCACCAACTCCCCAACCCTAGATATGCCTATTTCAGAACTTACTTTAATAAAAATAAAGTTTTCATTCTATCTTTTTTGGTCAGCATGACCTCTTAATCAAAGTTATCAACTTTATTTTATTTGGCTTCACATGCATACAAATCCTTTTCTTTTGTTCAGTTGCTTTTGTTGCAAAACATAATTTTTTTCATCCTGTTTTCATAAACTATGTGAAAATTTTGGGTTAATAATAATATAATTTTTATTTTTCCCTTTTTGGTTGAAGGTTTTGGCTCCACTTCCAATTGGGTTTGCAGTGTTCATGGTTCACTTGGCCACCATTCCCATCACTGGCACTGGAATCAACCCAGCAAGGAGCTTGGGATCTGCTGTTATTTTGAATGAGCAAAAGGCTTGGAATAATCATGTTAGTCCTCATTTTTTTACACTTCTTCAATGATCTTGTTTCAATAAGAATATCATAAAGCATCTCGACGTTTCAGATAAAGACACATTAATGTCGACATTATTAGATAAATATAGGGTTCCATCTCAAAATCAATGACAATGAATAGAATAACCTATGTATCTTATAGATTGTAAGGTCTCTATTCATCTTTTCAATGTGAGATCCTCAACCTACCCCTCAAGATGGTACCTCTTTGAGTTTACCATTCTTGGACCGAATATCCGTTTGAATTTTATGGACTCTGATATCATATAAACATGAGTTTTATCTCAAAAGTCAAAACCAACTGGCAATGAGTAGAATAACTCATGTATCTTATAAATTGTAAGGTCTCTTGGTCTCTCTTCTTCTTTTCGATGTAGGATCCTCAACGAACATTGATGGCTCAAATTTACAAATACATCGAAACCAAATATATTATCTGAAACTATAGAAATCATTATTGAACACTTGAAATATTTGTCTTGGGAACTAAATTTTATGGATTTTTGTTTGCAGTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCATTCTACCACCAATTCATCTTGAGAGCAGGTGCTGTTAAAGCTCTTGGGTCATTCAGAAGCAACCCACATGTGTGATCATATTTCTGTTTTTAATTAGTTCTTGGAGATTAAGAACCAAATTAAAACTAATAATTTGCCTTTTAACTTTTCTAAATTCCTATGCTTGCTTGTGACTTTCATGAATCTAATAGCTCTTTCTTTTGCCATTGGAATGGTTAAAAATAGTTTGTTTGTTTACATGACTTCCAGAGGTTAGGAGAAGGGTTATTTCTTAGGAATGGCAAAGTTTGATATTGTTATTTCTTTTCTAATCTTGATTTATGTGGAGCTTTCTATA

mRNA sequence

ATGGCAATGGCCGTAAGAAGCGACAAAGGTACGGAGCAGAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCAGAAAATCACTGCTCCTCTTCTCTTCTTTGCCTTCAATTTCCTCTCATTAAATGCCGCTCTTCGCTCCCCAACTTCGCACCATATTCATTCACAAATCCTCGCCGGAAATTGCTTCTCTCTCCTAGGGTTTCGATTTCTCTGTCTGTCTTTATGGCGAAGAATGCGGCGGAGCGCGGCTCGTTCTCGGCGAAGGACTACCAGGATCCGCCACCGGCGCCGCTCATCGACGCCGTGGAGATTACGAAGTGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTTTGCTTTTCCTTTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAATGTGGATCCTTGCGGTGGCGTTGGGATTCTGGGAATTGCTTGGGCATTTGGAGGAATGATCTTCGTTCTTGTTTACTGTACTGCTGGAATCTCAGGGGGCCACATAAACCCAGCAGTGACATTTGGGCTGCTGTTGGCCCGTAAAGTGTCACTGGTGCGGGCCGTGATGTACATGGTGGCCCAATGCTTGGGGGCCATAAGCGGAGTTGGGCTCGTAAAGGCCTTCCAGAAGGCCCACTTCCAGAAGCACGGCGGCGGGGCCAATGGGCTCGCCGACGGCTACACTGTCGGCGTCGGGCTGGCCGCCGAGATTGTCGGCACGTTTGTGTTGGTGTACACAGTCTTTTCTGCTACTGATCCCAAACGAAACGCCAGAGATTCTCATGTTCCTGTTTTGGCTCCACTTCCAATTGGGTTTGCAGTGTTCATGGTTCACTTGGCCACCATTCCCATCACTGGCACTGGAATCAACCCAGCAAGGAGCTTGGGATCTGCTGTTATTTTGAATGAGCAAAAGGCTTGGAATAATCATTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCATTCTACCACCAATTCATCTTGAGAGCAGGTGCTGTTAAAGCTCTTGGGTCATTCAGAAGCAACCCACATGTGTGA

Coding sequence (CDS)

ATGGCAATGGCCGTAAGAAGCGACAAAGGTACGGAGCAGAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCAGAAAATCACTGCTCCTCTTCTCTTCTTTGCCTTCAATTTCCTCTCATTAAATGCCGCTCTTCGCTCCCCAACTTCGCACCATATTCATTCACAAATCCTCGCCGGAAATTGCTTCTCTCTCCTAGGGTTTCGATTTCTCTGTCTGTCTTTATGGCGAAGAATGCGGCGGAGCGCGGCTCGTTCTCGGCGAAGGACTACCAGGATCCGCCACCGGCGCCGCTCATCGACGCCGTGGAGATTACGAAGTGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTTTGCTTTTCCTTTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATATCGGCAACAAGAATGTGGATCCTTGCGGTGGCGTTGGGATTCTGGGAATTGCTTGGGCATTTGGAGGAATGATCTTCGTTCTTGTTTACTGTACTGCTGGAATCTCAGGGGGCCACATAAACCCAGCAGTGACATTTGGGCTGCTGTTGGCCCGTAAAGTGTCACTGGTGCGGGCCGTGATGTACATGGTGGCCCAATGCTTGGGGGCCATAAGCGGAGTTGGGCTCGTAAAGGCCTTCCAGAAGGCCCACTTCCAGAAGCACGGCGGCGGGGCCAATGGGCTCGCCGACGGCTACACTGTCGGCGTCGGGCTGGCCGCCGAGATTGTCGGCACGTTTGTGTTGGTGTACACAGTCTTTTCTGCTACTGATCCCAAACGAAACGCCAGAGATTCTCATGTTCCTGTTTTGGCTCCACTTCCAATTGGGTTTGCAGTGTTCATGGTTCACTTGGCCACCATTCCCATCACTGGCACTGGAATCAACCCAGCAAGGAGCTTGGGATCTGCTGTTATTTTGAATGAGCAAAAGGCTTGGAATAATCATTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCATTCTACCACCAATTCATCTTGAGAGCAGGTGCTGTTAAAGCTCTTGGGTCATTCAGAAGCAACCCACATGTGTGA

Protein sequence

MAMAVRSDKGTEQSNGKWVGGVPNSAGAAENHCSSSLLCLQFPLIKCRSSLPNFAPYSFTNPRRKLLLSPRVSISLSVFMAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV
Homology
BLAST of Spg039685 vs. NCBI nr
Match: XP_022134214.1 (probable aquaporin PIP2-5 [Momordica charantia])

HSP 1 Score: 552.4 bits (1422), Expect = 3.0e-153
Identity = 275/287 (95.82%), Postives = 280/287 (97.56%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFS KDY DPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDH  NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQK+GGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAV MVHLATIPITGTGINPARSLGSAVILN
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           +QK WN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of Spg039685 vs. NCBI nr
Match: XP_022954319.1 (probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquaporin PIP2-5 [Cucurbita maxima] >KAG6602317.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033000.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 551.2 bits (1419), Expect = 6.7e-153
Identity = 275/287 (95.82%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFSAKDY DPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQK+GGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            Q  WNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Spg039685 vs. NCBI nr
Match: XP_023540772.1 (probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.5 bits (1399), Expect = 1.4e-150
Identity = 272/287 (94.77%), Postives = 278/287 (96.86%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFSAKDY DPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDH GNKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQK+GGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            Q  WNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Spg039685 vs. NCBI nr
Match: XP_038886208.1 (probable aquaporin PIP2-5 [Benincasa hispida])

HSP 1 Score: 520.4 bits (1339), Expect = 1.3e-143
Identity = 265/303 (87.46%), Postives = 275/303 (90.76%), Query Frame = 0

Query: 64  RKLLLSPRVSISLSVFMAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFI 123
           R + +SPRV +S+SVF          F AKDY DPPP PLID  EI KWSFYRALIAEF+
Sbjct: 15  RSIEVSPRVLVSVSVFCV--------FMAKDYYDPPPVPLIDGEEIRKWSFYRALIAEFM 74

Query: 124 ATLLFLYVTVLTVIGYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGG 183
           ATLLFLYVTVLTVIGYKS+TD +G KNVD CG VGILGIAWAFGGMIFVLVYCTAGISGG
Sbjct: 75  ATLLFLYVTVLTVIGYKSETDGMGKKNVDSCGEVGILGIAWAFGGMIFVLVYCTAGISGG 134

Query: 184 HINPAVTFGLLLARKVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADG 243
           HINPAVTFGLLL RKVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQK+GGGANGL+D 
Sbjct: 135 HINPAVTFGLLLGRKVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLSDD 194

Query: 244 YTVGVGLAAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITG 303
           YT  VGLAAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITG
Sbjct: 195 YTASVGLAAEIVGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITG 254

Query: 304 TGINPARSLGSAVILNEQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 363
           TGINPARSLGSAVI N+QKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN
Sbjct: 255 TGINPARSLGSAVIFNDQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 309

Query: 364 PHV 367
           PHV
Sbjct: 315 PHV 309

BLAST of Spg039685 vs. NCBI nr
Match: XP_008456476.1 (PREDICTED: probable aquaporin PIP2-5 [Cucumis melo])

HSP 1 Score: 515.0 bits (1325), Expect = 5.3e-142
Identity = 255/275 (92.73%), Postives = 264/275 (96.00%), Query Frame = 0

Query: 92  AKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNKNV 151
           AKDYQDPPP PLID VEI KWSFYRALIAEF+ATLLFLYVTVLTVIGYK++T  +G KNV
Sbjct: 2   AKDYQDPPPVPLIDGVEIRKWSFYRALIAEFMATLLFLYVTVLTVIGYKAETHGMGKKNV 61

Query: 152 DPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQ 211
           D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLL RKVSLVRAVMYMVAQ
Sbjct: 62  DSCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLGRKVSLVRAVMYMVAQ 121

Query: 212 CLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPK 271
           CLGAISGVGLVKAFQKAHFQKHGGGANG++DGYT+G GL AEIVGTFVLVYTVFSATDPK
Sbjct: 122 CLGAISGVGLVKAFQKAHFQKHGGGANGVSDGYTIGTGLVAEIVGTFVLVYTVFSATDPK 181

Query: 272 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWNNHWIFW 331
           RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN+QKAWNNHWIFW
Sbjct: 182 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNDQKAWNNHWIFW 241

Query: 332 VGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           VGPF+GAAIAAFYHQFILRAGAVKALGSFRSN HV
Sbjct: 242 VGPFLGAAIAAFYHQFILRAGAVKALGSFRSNSHV 276

BLAST of Spg039685 vs. ExPASy Swiss-Prot
Match: Q9SV31 (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 2.7e-133
Identity = 233/281 (82.92%), Postives = 258/281 (91.81%), Query Frame = 0

Query: 86  ERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 145
           ++ SFS KDYQDPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 146 IGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 205
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 206 MYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 265
           MYMVAQCLGAI GV LVKAFQ A+F ++GGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 266 SATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWN 325
           SATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPITGTGINPARSLG+A+I N+ KAW+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 326 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS PHV
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of Spg039685 vs. ExPASy Swiss-Prot
Match: Q8H5N9 (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.8e-130
Identity = 228/276 (82.61%), Postives = 250/276 (90.58%), Query Frame = 0

Query: 88  GSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIG 147
           G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK QTD   
Sbjct: 14  GEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASA 73

Query: 148 NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMY 207
           +     CGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKVSLVRA++Y
Sbjct: 74  SGADAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILY 133

Query: 208 MVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSA 267
           +VAQCLGAI GVGLVKAFQ A+F ++GGGAN LA GY+ G GLAAEI+GTFVLVYTVFSA
Sbjct: 134 IVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSKGTGLAAEIIGTFVLVYTVFSA 193

Query: 268 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWNNH 327
           TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS+G+AVI N +KAW+NH
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSIGAAVIFNNEKAWHNH 253

Query: 328 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 364
           WIFWVGPF+GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 254 WIFWVGPFVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of Spg039685 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 459.1 bits (1180), Expect = 4.5e-128
Identity = 226/282 (80.14%), Postives = 250/282 (88.65%), Query Frame = 0

Query: 82  KNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKS 141
           ++ A  G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK 
Sbjct: 8   ESGAGGGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKH 67

Query: 142 QTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSL 201
           QTD   +     CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LARKVSL
Sbjct: 68  QTDASASGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSL 127

Query: 202 VRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLV 261
           VRA++Y+VAQCLGAI GVGLVKAFQ A+F ++GGGAN LA GY+ G GL AEI+GTFVLV
Sbjct: 128 VRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLV 187

Query: 262 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQ 321
           YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSLG+AVI N+ 
Sbjct: 188 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKD 247

Query: 322 KAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 364
           K W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 248 KPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of Spg039685 vs. ExPASy Swiss-Prot
Match: Q9ATM8 (Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 2.3e-127
Identity = 227/286 (79.37%), Postives = 251/286 (87.76%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           + ++ A  G F+AKDY DPPPAPL+DA E+  WS YRA+IAEFIATLLFLYVTV TVIGY
Sbjct: 6   VVQSGAGGGEFAAKDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFLYVTVATVIGY 65

Query: 140 KSQTD-HIGNKNVD-PCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 199
           K QTD        D  CGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LAR
Sbjct: 66  KHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLAR 125

Query: 200 KVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGT 259
           KVSLVRA++YMVAQCLGA+ GVGLVKAFQ A+F ++GGGAN LA GY+ G GL AEIVGT
Sbjct: 126 KVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSRGAGLGAEIVGT 185

Query: 260 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVI 319
           FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIP+TGTGINPARSLG+AV+
Sbjct: 186 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVV 245

Query: 320 LNEQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 364
            N+ K W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 246 YNKDKPWDDHWIFWVGPLLGAAIAAFYHQYILRAGAIKALGSFRSN 291

BLAST of Spg039685 vs. ExPASy Swiss-Prot
Match: P43287 (Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 449.9 bits (1156), Expect = 2.8e-125
Identity = 224/287 (78.05%), Postives = 248/287 (86.41%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAK+      F  +DY+DPPP P  DA E+TKWS YRA+IAEF+ATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SL+RAV+YMVAQCLGAI GVG VKAFQ +++ ++GGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G+AVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           + K W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  +V
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Spg039685 vs. ExPASy TrEMBL
Match: A0A6J1BY62 (probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.5e-153
Identity = 275/287 (95.82%), Postives = 280/287 (97.56%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFS KDY DPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDH  NKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQK+GGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAV MVHLATIPITGTGINPARSLGSAVILN
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           +QK WN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of Spg039685 vs. ExPASy TrEMBL
Match: A0A6J1JQ27 (probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.2e-153
Identity = 275/287 (95.82%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFSAKDY DPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQK+GGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            Q  WNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Spg039685 vs. ExPASy TrEMBL
Match: A0A6J1GSM7 (probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.2e-153
Identity = 275/287 (95.82%), Postives = 281/287 (97.91%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAKNAAERGSFSAKDY DPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQK+GGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            Q  WNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Spg039685 vs. ExPASy TrEMBL
Match: A0A1S3C4L6 (probable aquaporin PIP2-5 OS=Cucumis melo OX=3656 GN=LOC103496419 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.6e-142
Identity = 255/275 (92.73%), Postives = 264/275 (96.00%), Query Frame = 0

Query: 92  AKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNKNV 151
           AKDYQDPPP PLID VEI KWSFYRALIAEF+ATLLFLYVTVLTVIGYK++T  +G KNV
Sbjct: 2   AKDYQDPPPVPLIDGVEIRKWSFYRALIAEFMATLLFLYVTVLTVIGYKAETHGMGKKNV 61

Query: 152 DPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQ 211
           D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLL RKVSLVRAVMYMVAQ
Sbjct: 62  DSCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLGRKVSLVRAVMYMVAQ 121

Query: 212 CLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPK 271
           CLGAISGVGLVKAFQKAHFQKHGGGANG++DGYT+G GL AEIVGTFVLVYTVFSATDPK
Sbjct: 122 CLGAISGVGLVKAFQKAHFQKHGGGANGVSDGYTIGTGLVAEIVGTFVLVYTVFSATDPK 181

Query: 272 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWNNHWIFW 331
           RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN+QKAWNNHWIFW
Sbjct: 182 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNDQKAWNNHWIFW 241

Query: 332 VGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           VGPF+GAAIAAFYHQFILRAGAVKALGSFRSN HV
Sbjct: 242 VGPFLGAAIAAFYHQFILRAGAVKALGSFRSNSHV 276

BLAST of Spg039685 vs. ExPASy TrEMBL
Match: A0A0A0KG37 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G405320 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.5e-139
Identity = 248/275 (90.18%), Postives = 263/275 (95.64%), Query Frame = 0

Query: 92  AKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHIGNKNV 151
           AKDYQDPPP PLID VEI KWSFYRALIAEF+ATLLFLYVTVLTVIGYK++T  +G KNV
Sbjct: 2   AKDYQDPPPVPLIDGVEIRKWSFYRALIAEFMATLLFLYVTVLTVIGYKAETHGMGKKNV 61

Query: 152 DPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQ 211
           D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLL RKVSLVRAVMYMVAQ
Sbjct: 62  DSCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLGRKVSLVRAVMYMVAQ 121

Query: 212 CLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPK 271
           CLGAISGVG+VKAFQKAHF+KHGGGANG++D Y++G GLAAEIVG+FVLVYTVFSATDPK
Sbjct: 122 CLGAISGVGIVKAFQKAHFEKHGGGANGVSDEYSIGTGLAAEIVGSFVLVYTVFSATDPK 181

Query: 272 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWNNHWIFW 331
           RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN+QK WNNHW+FW
Sbjct: 182 RNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNDQKTWNNHWVFW 241

Query: 332 VGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           VGPF+GAAIAAFYHQFILRAGAVKALGSFRS+ HV
Sbjct: 242 VGPFLGAAIAAFYHQFILRAGAVKALGSFRSSSHV 276

BLAST of Spg039685 vs. TAIR 10
Match: AT3G54820.1 (plasma membrane intrinsic protein 2;5 )

HSP 1 Score: 476.5 bits (1225), Expect = 2.0e-134
Identity = 233/281 (82.92%), Postives = 258/281 (91.81%), Query Frame = 0

Query: 86  ERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 145
           ++ SFS KDYQDPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 146 IGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 205
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 206 MYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 265
           MYMVAQCLGAI GV LVKAFQ A+F ++GGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 266 SATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILNEQKAWN 325
           SATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPITGTGINPARSLG+A+I N+ KAW+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 326 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS PHV
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of Spg039685 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 449.9 bits (1156), Expect = 2.0e-126
Identity = 224/287 (78.05%), Postives = 248/287 (86.41%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAK+      F  +DY+DPPP P  DA E+TKWS YRA+IAEF+ATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SL+RAV+YMVAQCLGAI GVG VKAFQ +++ ++GGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G+AVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           + K W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  +V
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Spg039685 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.6e-126
Identity = 225/287 (78.40%), Postives = 246/287 (85.71%), Query Frame = 0

Query: 80  MAKNAAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 139
           MAK+      F  +DY+DPPP P  DA E+TKWS YRA+IAEF+ATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 140 KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 199
           K Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 200 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGTFV 259
           SL+RAV+YMVAQCLGAI GVG VKAFQ +H+  +GGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFV 180

Query: 260 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVILN 319
           LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G+AVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240

Query: 320 EQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
           + K W++HWIFWVGPFIGA IAAFYHQF+LRA   K+LGSFRS  +V
Sbjct: 241 KSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Spg039685 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 444.1 bits (1141), Expect = 1.1e-124
Identity = 223/289 (77.16%), Postives = 250/289 (86.51%), Query Frame = 0

Query: 80  MAKN--AAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 139
           MAK+  A     F  +DYQDPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 140 GYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 199
           GYK Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSDTDAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 200 KVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGT 259
           KVSL RA++Y++AQCLGAI GVG VKAFQ +++ ++GGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 260 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVI 319
           FVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 320 LNEQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            N+ K W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  +V
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of Spg039685 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 444.1 bits (1141), Expect = 1.1e-124
Identity = 223/289 (77.16%), Postives = 250/289 (86.51%), Query Frame = 0

Query: 80  MAKN--AAERGSFSAKDYQDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 139
           MAK+  A     F  +DYQDPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 140 GYKSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 199
           GYK Q+D      VD CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSDTDAG-GVD-CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 200 KVSLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKHGGGANGLADGYTVGVGLAAEIVGT 259
           KVSL RA++Y++AQCLGAI GVG VKAFQ +++ ++GGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 260 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGSAVI 319
           FVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 320 LNEQKAWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 367
            N+ K W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  +V
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134214.13.0e-15395.82probable aquaporin PIP2-5 [Momordica charantia][more]
XP_022954319.16.7e-15395.82probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquapori... [more]
XP_023540772.11.4e-15094.77probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo][more]
XP_038886208.11.3e-14387.46probable aquaporin PIP2-5 [Benincasa hispida][more]
XP_008456476.15.3e-14292.73PREDICTED: probable aquaporin PIP2-5 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9SV312.7e-13382.92Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1[more]
Q8H5N94.8e-13082.61Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=... [more]
Q84RL74.5e-12880.14Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Q9ATM82.3e-12779.37Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1[more]
P432872.8e-12578.05Aquaporin PIP2-2 OS=Arabidopsis thaliana OX=3702 GN=PIP2-2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1BY621.5e-15395.82probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV... [more]
A0A6J1JQ273.2e-15395.82probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1[more]
A0A6J1GSM73.2e-15395.82probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=... [more]
A0A1S3C4L62.6e-14292.73probable aquaporin PIP2-5 OS=Cucumis melo OX=3656 GN=LOC103496419 PE=3 SV=1[more]
A0A0A0KG373.5e-13990.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G405320 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G54820.12.0e-13482.92plasma membrane intrinsic protein 2;5 [more]
AT2G37170.12.0e-12678.05plasma membrane intrinsic protein 2 [more]
AT2G37180.12.6e-12678.40Aquaporin-like superfamily protein [more]
AT3G53420.11.1e-12477.16plasma membrane intrinsic protein 2A [more]
AT3G53420.21.1e-12477.16plasma membrane intrinsic protein 2A [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 252..270
score: 53.95
coord: 328..348
score: 50.21
coord: 166..190
score: 62.61
coord: 203..222
score: 49.1
coord: 116..135
score: 45.9
coord: 288..310
score: 58.26
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 108..345
e-value: 2.8E-82
score: 275.8
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 120..345
e-value: 1.5E-63
score: 212.6
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 116..348
e-value: 2.93872E-77
score: 235.611
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 101..366
e-value: 6.8E-87
score: 292.7
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 108..350
NoneNo IPR availablePANTHERPTHR45687:SF66AQUAPORIN PIP2-1coord: 84..363
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 84..363
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 184..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039685.1Spg039685.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity