Spg039659 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg039659
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	vacuolar protein sorting-associated protein 25
Location	scaffold10: 45456681 .. 45459372 (+)
RNA-Seq Expression	Spg039659
Synteny	Spg039659

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATTTTCCTGTAGGCAACAGCATAGTTATGAAGTTAGTGTTTCTTCGACAGTTCTGTTTAGTCATTATCTTTGTGAAGTTTTCATGCCGTATGGTATTTTATAGCTATAGCTTCGCCATGTCTAGTTTAGTTTTCAGCTATCGGCATTGATCTGATTCATAAAATACAGAAACTTGATTCTACTTTTACTTGCTCATCATAGAACAATTTGTATTTCAGATAAGTTTATATATTTTTGTAACGACAAGCTTGTATTAGTTTGCAAGTCTCTAGTCTCATCAAATCATTGACACCTCAAAAATAGTTATGCATCTGACACAAAATGGCTAGGTCTGTCATGTTTATGAACTCTGCATTATTTTGTTTATGTATTTTATTGATGTTAAATTTCTTCCTTTTCATCTGATATTTCTCAGTTCTAGAGATAAGATGCATCTGTAGGATTCACTTACAGAGGAAGCAATCATAAAAATGCAGAAATTGGGGGATTTCAAGTTACCGCATTTCTTCAATTACCCACCATATTTCACGTAATTTTCCTTCTGTCTGAGTTGCCATTGCTTTACATCATTTTAAGCTGCAACAAGTAACTTGGAAGCTGTCTAAGTCGATTTCTGTTGATAATGATATATTGGTTATAATGGCTGTTTTTTGAGTGAATGAAACTAACAGTGCATTGCTCAACTAGTATTTACATAACACTAATTAAGATTAATAGGATATTGGATCACTGTACTTTCCCCATAAGCCTCAGAAATATGAATGTAATAAATACTAGCTTACTGCAATTTCTGCAATTATTATTGATAACTCTAAATTGTTTCATTTTTTGTCAAGTTTGTCTTTTGTCTTGAGTTGCACATATAACCCTTTATTGCATGCCTTCTCAGTTTGCAACCAGTAAGAGAAACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTGTTCTCGACTACTGCCGACAACAAAAGATATTTGTCATTGGACTCGAAGAGGAATTTCCTCTATTTTCAAATTCTGTCATCCAGAGTAAATTTCATGAACTTGTTTGGTCCTAATTGGTTGTTAAAACCAATTTCTACATGCATATATAAATATCTTTCTTAAAATGGTTAATTACTGTAGCATCTTGAACAGTGTGAAAAAGATTATGATATTGTCCTCATTCTATGCAAACCCTTTCACTCTATGGAGCATAATATGTAATTGAGAATTGGTGTTTAAGACTCAGTCATGAAGCTATGTATCTCTATTTTAGTTGTTTTATGTGTTTTACAATGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTTAGGCCCTGCTAGTTTAGTTATATAATTCTATCTCTTTACTGTTTCCATTCATTCCCATGGGGAAAAATTGGCAATTAGGTTACTTTTCATTGTCCAATAGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGACTAAATACTTTATTAACTGTAGTGAAAATTAGATGGGTGTAAGATGAGTTTGGAATGACTTTTGAAAGGGTGAAAATTGCTATTATCCCATTAAAAGCAGTTTTCAAATTTCCTATAGTATTTAGTTAGATAATCGAAAGTGCTTTTGAAAAATTCTTGTTCTAAACTTACTGCCATATTAAATAGGTGAAGAATAATGGACTTGAAGATAGCGTTATGACAGTGGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGGTACTTCCCTTGCTTCTACTGAAGTGATTTAGATCTTCGAATGATTTCTAAATCTATTTTTCGATATGTTACATTGATTTTGATTTTAAACTTTCAAGTTTCAAACTTTCATTTTTGATTTTTAAACTTGCTCTTATATTTCTAAATGTTCTACTCTGATACAGTCTTTGGTCCTTGTCAAATCAGTAGATAATATTTAACAAATTTGATGCTGAATGGAATGCAAGAGACTTGAAACATGAATTCAATTTATGTGATGTCATGTATGAATTTTGTTAAATGCTCTAAAAAGTTGCACAACAAAGACTAAAATAACTTTTTATGCAAAATTCATGAACCAAATTGGAGTGTTTTGTCTGAGGACCAAAACAGATTGATGTAAATATTCCTGAATCACATTTATTTGTTCTGTGCTTAAATACTACATCCTGGAATAACATTCAAACCTTATTTTGACAAACACCACTAAACTTATTTTTTTGTTTTATGCATCAGAGCTTCAAGGGATTGACCGTACAATTTTAATGAGAGCATTGAAGCTACTCGAACAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGCATTAAATTCTCTGTATAACTATAATATTTATTCACATTACCACTCAGTTTTTGAACAGTCTCAATGTGAGTTTTCTAGTTCAATCTTCTAAATGATACTCTTCTTTAGTTTCCCTGCCAATTCAATCTGGGACTATGGAGAGTTCTTTTATTCTTGGGAAGATTTGGTGCAGAGTTCAGTCTAATGTAACCAGAAGTCTTCAAATAAAACTTAAGAAACTATGCTTGGGACGGCCTAGTTTATATGCACCTTGATTAATCTCATGAGATAGTTGTTC

mRNA sequence

ATGCCATTTTCCTGTAGGCAACAGCATAGTTATGAAGTTAGTGTTTCTTCGACAGTTCTGTTTAGTCATTATCTTTGTGAAGTTTTCATGCCGTATGGATTCACTTACAGAGGAAGCAATCATAAAAATGCAGAAATTGGGGGATTTCAAGTTACCGCATTTCTTCAATTACCCACCATATTTCACGTAATTTTCCTTCTGTCTGAGTTGCCATTGCTTTACATCATTTTAAGCTGCAACAATTTGCAACCAGTAAGAGAAACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTGTTCTCGACTACTGCCGACAACAAAAGATATTTGTCATTGGACTCGAAGAGGAATTTCCTCTATTTTCAAATTCTGTCATCCAGAGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAGAATAATGGACTTGAAGATAGCGTTATGACAGTGGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGACCGTACAATTTTAATGAGAGCATTGAAGCTACTCGAACAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGCATTAAATTCTCTGTATAA

Coding sequence (CDS)

ATGCCATTTTCCTGTAGGCAACAGCATAGTTATGAAGTTAGTGTTTCTTCGACAGTTCTGTTTAGTCATTATCTTTGTGAAGTTTTCATGCCGTATGGATTCACTTACAGAGGAAGCAATCATAAAAATGCAGAAATTGGGGGATTTCAAGTTACCGCATTTCTTCAATTACCCACCATATTTCACGTAATTTTCCTTCTGTCTGAGTTGCCATTGCTTTACATCATTTTAAGCTGCAACAATTTGCAACCAGTAAGAGAAACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTGTTCTCGACTACTGCCGACAACAAAAGATATTTGTCATTGGACTCGAAGAGGAATTTCCTCTATTTTCAAATTCTGTCATCCAGAGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTGAAGAATAATGGACTTGAAGATAGCGTTATGACAGTGGAGGAAATACGTTCAGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGACCGTACAATTTTAATGAGAGCATTGAAGCTACTCGAACAGAAGGGGAAGCTTGCAATTTTCAAGGGTTCTTCAACAGATGACGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MPFSCRQQHSYEVSVSSTVLFSHYLCEVFMPYGFTYRGSNHKNAEIGGFQVTAFLQLPTIFHVIFLLSELPLLYIILSCNNLQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV

Homology

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BLAST of Spg039659 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 311.2 bits (796), Expect = 7.6e-81
Identity = 153/159 (96.23%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNSVI+RSLSHEAR+AFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 2.9e-80
Identity = 151/159 (94.97%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNSVI+RSLSHEAR+AF+SA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFISA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           L+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. NCBI nr
Match: XP_038891553.1 (vacuolar protein sorting-associated protein 25 [Benincasa hispida])

HSP 1 Score: 309.3 bits (791), Expect = 2.9e-80
Identity = 151/159 (94.97%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNS I+RSLSHEAREAFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSEIERSLSHEAREAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMT+EEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTIEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRT+LMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTVLMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 307.8 bits (787), Expect = 8.4e-80
Identity = 150/159 (94.34%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEEEFP+F+N+ I+RSLSHEAREAFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIFTNTAIERSLSHEAREAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEE+RSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEMRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 307.4 bits (786), Expect = 1.1e-79
Identity = 152/159 (95.60%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPV++TREKQIQLWKEL+LDYCR QKIFVIGLEEEFPLFSNSVI+RSLSHEAR AFLSA
Sbjct: 21  LQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLFSNSVIERSLSHEARVAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-75
Identity = 135/159 (84.91%), Postives = 149/159 (93.71%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYC+ QKIF+IG+EE+FPLFSNS I RSLSHEARE FLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLFSNSAIDRSLSHEARETFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           +V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGLEDSVMTVEEIRSG ES GTE
Sbjct: 81  IVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGLEDSVMTVEEIRSGTESLGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 141 LQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 4.1e-29
Identity = 68/172 (39.53%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 69  ELPLLYIILSCNNLQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEE--EFPLFSNSVI 128
           E P  Y       LQP  +TR+KQ+  W  LVL YCRQ K++ + L E  E PLF+N  I
Sbjct: 4   EWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKKI 63

Query: 129 QRSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVM 188
           QR LS E+ +  L  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV 
Sbjct: 64  QRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSVF 123

Query: 189 TVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 239
           T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 124 TLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]

BLAST of Spg039659 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 122.5 bits (306), Expect = 6.6e-27
Identity = 64/172 (37.21%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 69  ELPLLYIILSCNNLQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEE--EFPLFSNSVI 128
           E P  Y       LQP  +TR+KQ+  W  LVL YCR +K++ + + E  E P+F+N  I
Sbjct: 4   EWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKKI 63

Query: 129 QRSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVM 188
           +R LS EA +     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV 
Sbjct: 64  ERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSVF 123

Query: 189 TVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 239
           T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 124 TLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of Spg039659 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.3e-26
Identity = 62/174 (35.63%), Postives = 105/174 (60.34%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFP----LFSNSVIQRSLSHEAREA 141
           +QP+  TR+KQ Q+W++L+L YCR  KI+ + + E       LF+N  I R LS EA ++
Sbjct: 20  IQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNNEKINRKLSREALKS 79

Query: 142 FLSALVSDGRAEWLDK-------------GHRKCLILWHRIQDWADIILHFVKNNGLEDS 201
            +  ++ +G AEW+DK              + + LI+W +  +WA +I  +V + GL ++
Sbjct: 80  IIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWASLIYKWVADCGLLNT 139

Query: 202 VMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 239
           V+TV EI++G +S+  E   ++ TILM++LK+LE++ K   F  S  ++ G+KF
Sbjct: 140 VLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQDENVGVKF 191

[Close]

BLAST of Spg039659 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 6.8e-24
Identity = 63/172 (36.63%), Postives = 93/172 (54.07%), Query Frame = 0


Query: 69  ELPLLYIILSCNNLQPVRETREKQIQLWKELVLDYCR--QQKIFVIGLEEEFPLFSNSVI 128
           E P  Y       LQP  +TR+KQ+  W  LVL +CR  +Q    +   +E PLF+N  +
Sbjct: 6   EWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKL 65

Query: 129 QRSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVM 188
           QR L  E+ +  L  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV 
Sbjct: 66  QRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVF 125

Query: 189 TVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKF 239
           T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 126 TLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]

BLAST of Spg039659 vs. ExPASy TrEMBL
Match: A0A5A7UHJ5 (Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001240 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.7e-81
Identity = 153/159 (96.23%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNSVI+RSLSHEAR+AFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy TrEMBL
Match: A0A1S3BKR8 (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.7e-81
Identity = 153/159 (96.23%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNSVI+RSLSHEAR+AFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy TrEMBL
Match: A0A0A0L1R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.4e-80
Identity = 151/159 (94.97%), Postives = 158/159 (99.37%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEE+FPLFSNSVI+RSLSHEAR+AF+SA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSVIERSLSHEARQAFISA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           L+GIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy TrEMBL
Match: A0A6J1CUP8 (vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN=LOC111014756 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 4.1e-80
Identity = 150/159 (94.34%), Postives = 157/159 (98.74%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYCR QKIFVIGLEEEFP+F+N+ I+RSLSHEAREAFLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIFTNTAIERSLSHEAREAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEE+RSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEMRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. ExPASy TrEMBL
Match: A0A6J1L2J9 (vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111498518 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 5.3e-80
Identity = 152/159 (95.60%), Postives = 156/159 (98.11%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPV++TREKQIQLWKEL+LDYCR QKIFVIGLEEEFPLFSNSVI+RSLSHEAR AFLSA
Sbjct: 21  LQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLFSNSVIERSLSHEARVAFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE
Sbjct: 81  LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 141 LQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Spg039659 vs. TAIR 10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 284.6 bits (727), Expect = 7.1e-77
Identity = 135/159 (84.91%), Postives = 149/159 (93.71%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYC+ QKIF+IG+EE+FPLFSNS I RSLSHEARE FLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLFSNSAIDRSLSHEARETFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           +V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGLEDSVMTVEEIRSG ES GTE
Sbjct: 81  IVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGLEDSVMTVEEIRSGTESLGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 141 LQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Spg039659 vs. TAIR 10
Match: AT4G19003.1 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 284.6 bits (727), Expect = 7.1e-77
Identity = 135/159 (84.91%), Postives = 149/159 (93.71%), Query Frame = 0


Query: 82  LQPVRETREKQIQLWKELVLDYCRQQKIFVIGLEEEFPLFSNSVIQRSLSHEAREAFLSA 141
           LQPVR+TREKQIQLWKEL+LDYC+ QKIF+IG+EE+FPLFSNS I RSLSHEARE FLSA
Sbjct: 21  LQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLFSNSAIDRSLSHEARETFLSA 80

Query: 142 LVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTE 201
           +V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGLEDSVMTVEEIRSG ES GTE
Sbjct: 81  IVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGLEDSVMTVEEIRSGTESLGTE 140

Query: 202 LQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 241
           LQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 141 LQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

The following BLAST results are available for this feature:

BLAST of Spg039659 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008448627.1	7.6e-81	96.23	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
XP_004146105.1	2.9e-80	94.97	vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp...	[more]
XP_038891553.1	2.9e-80	94.97	vacuolar protein sorting-associated protein 25 [Benincasa hispida]	[more]
XP_022145259.1	8.4e-80	94.34	vacuolar protein sorting-associated protein 25 [Momordica charantia]	[more]
XP_022949655.1	1.1e-79	95.60	vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557...	[more]

BLAST of Spg039659 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZC9	1.0e-75	84.91	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6AX45	4.1e-29	39.53	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2...	[more]
Q6NWF4	6.6e-27	37.21	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P...	[more]
Q55GD9	3.3e-26	35.63	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44...	[more]
Q9CQ80	6.8e-24	36.63	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25...	[more]

BLAST of Spg039659 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UHJ5	3.7e-81	96.23	Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. m...	[more]
A0A1S3BKR8	3.7e-81	96.23	vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365...	[more]
A0A0A0L1R7	1.4e-80	94.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1	[more]
A0A6J1CUP8	4.1e-80	94.34	vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1L2J9	5.3e-80	95.60	vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Spg039659 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19003.2	7.1e-77	84.91	E2F/DP family winged-helix DNA-binding domain	[more]
AT4G19003.1	7.1e-77	84.91	E2F/DP family winged-helix DNA-binding domain	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 164..239 e-value: 2.2E-25 score: 90.1
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	1.10.10.570		coord: 51..162 e-value: 1.5E-23 score: 85.0
IPR008570	ESCRT-II complex, Vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 82..206 e-value: 3.8E-37 score: 127.7
IPR008570	ESCRT-II complex, Vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 42..239
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 81..168
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 163..238

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg039659.1	Spg039659.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071985	multivesicular body sorting pathway
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005198	structural molecule activity