Spg038622 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg038622
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionFumarate hydratase
Locationscaffold12: 4146809 .. 4152365 (-)
RNA-Seq ExpressionSpg038622
SyntenySpg038622
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATCAAACACTCTCTTCTTATAAAGTCAGAGTCTCAAACGATTGGCTTAGTTGACATCTAGCAAGTCTGAGCATAACTTAATGTATAAAATCACATATTACTTTTCAAGATCGAAGGTTTGATCCTTTGCCAAGATGAGCAAAGTTTAGTGGTAATTGGTATCTTATCTTGATCAAGAGGTCAAATGTTTAAATCTCACGTCCACACGAGTCGAAATAAAAAAAATAATAAAAAAAATAAAAATCAGTTAGGCGCTTTGGTGGGCCGTCACAATTGGGCCTAAAGGAAGAAAAGTCCGATCTCAGGCAGCCGCAGCCATGAAAGCCACTTCCACTTTCACTTTCCAAACATTTGTTTCGTCAAAATAAATCGCCGTATTCTCTCTCAACACCTCTGATCCTCTGACGTCACCCGCCATTTCTCCGCCGCCTTATCTCCGGCCGTTCCTTCCATCGAGGATTCCACCTTCCCAACCTCCATTGTCACTCGCTTTCCTCTTCTTCAACACCACTCTCTCATTCTTCTTCCCCGAACTGATATCCGCTTCTAGCTCAGAAGAATGGCGGCCCTCTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTTGGATTCGCGCGCTGTGCTTCTACGTCTTTCAGATCTTACTCCACGGCTTTTCGCGAGGAAAGAGATACGTTTGGACCGATCAACGTTCCCTCCGACAGGTTCGTCTCTCTCGTTCATCTGCTTTTTGGATTTACTTGAGTATTAATTGCTCTGTGTTTAATTTTTCCGGAGGAAACTGTGATAAGTGAAGCGAAATGAGTTTTCATGAACTGTGTTGTTTGCCTTTTAGATTGTGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTTGAGATCGGAGGAGACCGTGAGCGAATGCCTGAACCTATTATTCGCGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTTCATTCGTTTTCAAATTAGGCGTAGTTTGATGTTTGATTCTTTGTGCTACAGTTCGTGTTTTACGATTGAGATAAACATGGAATGGAATACGGTTTTGGAAATGGTTCTACTGTTCATCGTGAGAATGTTTTTGTAGGTAAACATGGAATACGGTCTTGATCCGACCATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCTGAGGGAAAGTTGAACGATCATTTTCCACTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCGAATGAGGTTAGGACAACTCTGTTTTATTTTGATCGTCAGGTGTTGAGAGCGAAGGTTTATTTATTAAGTATCGTATTGCAAACGTACGTACTTCGCATATTTTATTATTTGGCGTCTATCTCTTTCTTTGATCGTGTTGTCTGGTTACTATGGAAATTATCCAAGGAAGCATGAAAGGCTAAGTCGCTGCTTCTATATTATGTATGGGTTCTTACAAGCGGCAGACATGATGACAGATGGTATTGAATCCCCTATGATCCTCCAGTCCGGAGGGAGAGATTTGCCTCCCATGGATAATTAGCTTCAGTCAGTAGCTTGAATCTCTGGCAATGTCCAGATCAAATTTCCCCTGAACTCCATGGCTAGTAGAGTTTATAGTACCATGTAACACGAAGTAGGCAGCTTTGAAACTAGTGGTTATTGATGGTTGAATTTAAGATGCTGTAAGATGACAACATTCTTCTTTCCCCACCTCCCTTTCCTACGTTGAGTCAAATTCACCTAGTTTTCTTTAAGTATAATCTCTTGTATAAGAAAAGCATTTCCTCCATAAAATATATATTGTCTTCCTTAATATTTGAACTGTCCCATAAGAATGTTATCTGCACATGATAGCAGAACATAGGGCAGTCTATTAAGTAATTTTCTTAGAATGTCTCTTAGGGTATATTTTGTTAGTTGATGACTCAAATGGTAGAAAATACGGACCTCTACTCTTTACAATTACTGAACATCTTATTCCTGTGGCAGGTCATTGCTAACAGAGCATCTGAGATCCTTGGTCATAAACGTGGTGAGAAGTTTGTTCATCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTGAGCTTTTCCTTTCTTAAGTTATTTTGTCTATTGCTATTCAATTCTAACTCTGTTTTTGTAACTAACCGATAAACTTCAGCAGTTTTCCGTGGCCAAGCTTCTTCATTTTTCCATTGCAATTTCAATAGCCAAACTTTATATTTTACTATTTAGACCATGAAAAAACTACTCTCATTGTCTTAACTGACAGGATTTTTTTCTCCACACTTCTGATTTCTACTGTTTTCCTTTTATAGGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCGTACCAAAATTGAAAACTTTACATTCATCACTATACTCAAAGGTTTGATATTTAGGAGAGCTATGTTTCCCAGAATTTCAATGTTATTATTTGCACCTTCACATGCTGTCGATGATCCGAGCTTAAGTTATTTACACAAACCTCTTTGAGTCGTAATGTTAGGACATGTTTGTTTGATTGCATGGACTAAGCTAAATGTGTAAAAATCGGAAAACATCAGGAGATCTTAACATGGTTTGGATCTATTGTACCACCTGTTAGATTCAGCATTTTTTAACTGAGTTGTGTGGTAGTAATATATGTGTTTATTTACACTCCCAGCTCGTACCTTGCATTCTATTTTCCATTTGCACTTTCATAAGTTTGTAATTCCTTATATTACTCTGTTATGATCCAAACAGTCTATAGAATTCAAAGATATTGTTAAGATTGGGCGCACTCACACTCAAGACGCAACACCATTAACTCTTGGGCAGGAGTTCAGTGGCTATACCACACAAGTATGCCCTCTCTCTTTGTGCTTGTGGAAGTTTTGGGTTCCTAATGTCTATTGAAAAGAAGAAAGAGGGTATGATGCATATGAATAATTTTCTGATTGGTGTGCACTTGGACTACAGGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGGTCACAACATGTGTAACGTTTCCAGGTATTTTGTCGGTTCTTTTGGCTATTTATGTTTTAACACTTCAGAATCCCATTTGTGCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGGTATATTTTCTTCAGAATTAGTTTGAATAAGAAAAGTAATCTAGTTTTCGGATATTGTACTTGACGATCAGAATGATGAACACATGTTATTATTTTGAGATTGGCGCTGTCTGTTGTTCAGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAAATTGCCTTTTGTGACAGCAGAAAACAAGTTTGAAGCCTTGGTTAGTCATTGAATGAGAATCATCTCCACTCTCTTTTAATTTTTATTCCACAGCTTGGTGTTGCATACTTTCACGCTTGGAACCACTTTTTTATGCTAAAATGTTTTGTTTCTTACCAGGCTGCACACGATGCCTTTGTTGAAACCAGTGGGGCTCTTAACACAGTTGCTACTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGGTTTATATGCATTAATTTTGTGAATCTTGTCTCTTTATTTTTTATAGTTCTCTAGCTAATATTTTCCCCTTCATTTCATGTATACAGTGGTCCACGTTGTGGTCTTGGAGAACTTATTCTTCCCGAAAATGAGCCTGGCAGTAGTATTATGCCAGTAATACTACTCTCTCTCTCTCTGTATTTGGGGACGTGTGCATGTATGTCTGGGCTTGTGTAACCTAAACAATTTTTTTGGTTTCAGGGGAAGGTGAACCCTACTCAATGCGAGGCTCTCACTATGGTCTGTGCTCAGGTAAAGTACTTTCTACTTTCTAGCATAGTTTATTATGGGGTGCTGAAAGAGGAATTAGATAGTATAGATCCAATCACCCGACTCATTATGTTAGTTTCGTTTTGTACCCCCAAAAAAGTTTGGTCAATAAGCCTACGCATATTCAGATTCTCAGTGATTAGTTTCATATATGTATATATTGTCATTCTAGAACTTGAAGGATGATATGAAAATTCTTCTTGTTTTACAGGTCATGGGAAACCATGTGGCAGTAACTGTTGGTGGGTCGAATGGTCACTTTGAACTTAATGTATTTAAACCAATGATCGCCAATGCTCTTTTGCATGTAAGTTCTATTATTATCTCTCCACCGTCTGCTATGAGATTCATATCATATTGTCGACCTGTTTTTGGTCTTCAAATGACGTGTGTCTATATATTATATATATATGTGGAGAGAAAGTGAGAGAGAGAGTTGGAAAAAAAACTTCTGGATTCATCAAAACCTTCATCTCCAATGCTGGCTCTTTTTATTGGCAGTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAAAAGAACTGTGTGAGGGGAATTCAAGCTAACAAGGAAAGAATTTCGAAATTACTCCGTGAGGTAGTTTTATATTAATATTTGAAAAATACTTCTATACTTCACTGTTTGCATACAAGGTTTCGGCTGATGATTCTTGTTGTTATTTCTGCACGTAAATTCGACCACATTGCAGTCATTGATGCTTGTCACAGCTTTGAATCCTGTAAGTATTCGTCAACTAGAAAGCTCTCATTTTCCATAGGCTTGGTACTTTGTTTAATAATATGTCCCTGCATATTGTCTCCAGAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCTCACAAGGAAGGTTCCACTTTGAAGGTATGAATCGTATTTATATTTTAGATTCAAATCTTATTTCACCATCATGTCACTAATTTCAAATAAATCGTTTTTAACTTATAGATGATATAGGTGAATAAGATGTCTACCTAACAAGTGTTTGAAGTCAGATATTGTTGGTTTTAGTGTCAAGTCTTCAGACGTATGATTAAAGAAAAATTAGCAAGGATTGAAATAGTTTTTAACGTTAGTTAAAAGGACACAAAACATTTGAAAAATTGGAGACCGAAATGGAATAAATATGAGAATACATAGACCAAAATTAGGATTCAAACTTGAATTTTATTGCCTTACGATTATGCAGGAAGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTCGATAAATATGTGGTGCCTGAGAAAATGATTGGTCCATCTGATTGATAAAAGCTAAAGCTTCTTCTGTGATTGGATCATTGTTTCATCAAAATCATTTTTATAGGTACTCTCATTTTCCCTTGGGAGGAAGAAGAAATCCATTTTTATGAGGATTGAACTGGATAATAAAAAGAGACCACAGAGAAGAGGAAATTGTGGTCATTCCTCTTCTACTTTCAAAAGATTTTCTTAGCGGAAGTAATTAATAATGTATTTGAGACATTGGTTCTTGTGTACGGCCATTATTGGCTCTTTGACGATTGAGCCTTCTTTTTTCATTCAAACGGAACGATATAACATTAATCAATAGGCATTTGAGCCAACTCATTTGTTGCCATCTTACAGT

mRNA sequence

ATGGCGGCCCTCTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTTGGATTCGCGCGCTGTGCTTCTACGTCTTTCAGATCTTACTCCACGGCTTTTCGCGAGGAAAGAGATACGTTTGGACCGATCAACGTTCCCTCCGACAGATTGTGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTTGAGATCGGAGGAGACCGTGAGCGAATGCCTGAACCTATTATTCGCGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATACGGTCTTGATCCGACCATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCTGAGGGAAAGTTGAACGATCATTTTCCACTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCGAATGAGGTTAGGACAACTCTGTTTTATTTTGATCGTCAGGTCATTGCTAACAGAGCATCTGAGATCCTTGGTCATAAACGTGGTGAGAAGTTTGTTCATCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCGTACCAAAATTGAAAACTTTACATTCATCACTATACTCAAAGTCTATAGAATTCAAAGATATTGTTAAGATTGGGCGCACTCACACTCAAGACGCAACACCATTAACTCTTGGGCAGGAGTTCAGTGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAAATTGCCTTTTGTGACAGCAGAAAACAAGTTTGAAGCCTTGGCTGCACACGATGCCTTTGTTGAAACCAGTGGGGCTCTTAACACAGTTGCTACTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGAGAACTTATTCTTCCCGAAAATGAGCCTGGCAGTAGTATTATGCCAGGGAAGGTGAACCCTACTCAATGCGAGGCTCTCACTATGGTCTGTGCTCAGGTCATGGGAAACCATGTGGCAGTAACTGTTGGTGGGTCGAATGGTCACTTTGAACTTAATGTATTTAAACCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAAAAGAACTGTGTGAGGGGAATTCAAGCTAACAAGGAAAGAATTTCGAAATTACTCCGTGAGTCATTGATGCTTGTCACAGCTTTGAATCCTAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCTCACAAGGAAGGTTCCACTTTGAAGGAAGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTCGATAAATATGTGGTGCCTGAGAAAATGATTGGTCCATCTGATTGA

Coding sequence (CDS)

ATGGCGGCCCTCTATGTTCTGTCCCGGCGATCTTCGCCTTCGCACCTGTTTGGATTCGCGCGCTGTGCTTCTACGTCTTTCAGATCTTACTCCACGGCTTTTCGCGAGGAAAGAGATACGTTTGGACCGATCAACGTTCCCTCCGACAGATTGTGGGGAGCTCAAACGCAGAGATCGTTGCAGAACTTTGAGATCGGAGGAGACCGTGAGCGAATGCCTGAACCTATTATTCGCGCCTTTGGCGTACTTAAAAAGTGTGCTGCCAAGGTAAACATGGAATACGGTCTTGATCCGACCATTGGGAAAGCCATAATGCAAGCAGCCCAAGAAGTAGCTGAGGGAAAGTTGAACGATCATTTTCCACTAGTTGTATGGCAAACTGGAAGTGGCACTCAGAGTAACATGAATGCGAATGAGGTTAGGACAACTCTGTTTTATTTTGATCGTCAGGTCATTGCTAACAGAGCATCTGAGATCCTTGGTCATAAACGTGGTGAGAAGTTTGTTCATCCGAATGACCATGTTAACCGGTCACAATCTTCTAATGATACATTCCCAACTGTAATGCACATAGCAGCTGCAACAGAAATTAATTCCAGATTCGTACCAAAATTGAAAACTTTACATTCATCACTATACTCAAAGTCTATAGAATTCAAAGATATTGTTAAGATTGGGCGCACTCACACTCAAGACGCAACACCATTAACTCTTGGGCAGGAGTTCAGTGGCTATACCACACAAGTGAAGTATGGTATTGACAGAGTCTTGTGCACTCTACCTCGAATGTATCAGCTTGCCCAAGGTGGAACTGCTGTTGGAACTGGATTGAACACAAAGAAAGGATTTGATGTGAAGATAGCTGCAGCAGTAGCTGAAGAAACAAAATTGCCTTTTGTGACAGCAGAAAACAAGTTTGAAGCCTTGGCTGCACACGATGCCTTTGTTGAAACCAGTGGGGCTCTTAACACAGTTGCTACTTCTTTGATGAAGATTGCCAATGACATACGCCTGTTGGGAAGTGGTCCACGTTGTGGTCTTGGAGAACTTATTCTTCCCGAAAATGAGCCTGGCAGTAGTATTATGCCAGGGAAGGTGAACCCTACTCAATGCGAGGCTCTCACTATGGTCTGTGCTCAGGTCATGGGAAACCATGTGGCAGTAACTGTTGGTGGGTCGAATGGTCACTTTGAACTTAATGTATTTAAACCAATGATCGCCAATGCTCTTTTGCATTCATTGAGATTATTAGGGGATGCTTCTGCTTCATTTGAAAAGAACTGTGTGAGGGGAATTCAAGCTAACAAGGAAAGAATTTCGAAATTACTCCGTGAGTCATTGATGCTTGTCACAGCTTTGAATCCTAAAATTGGTTACGACAATGCTGCTGCAGTTGCAAAGACAGCTCACAAGGAAGGTTCCACTTTGAAGGAAGCTGCATTGAAGCTTGGAGTTCTAAGCGAGGAAGATTTCGATAAATATGTGGTGCCTGAGAAAATGATTGGTCCATCTGATTGA

Protein sequence

MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDFDKYVVPEKMIGPSD
Homology
BLAST of Spg038622 vs. NCBI nr
Match: XP_022155265.1 (fumarate hydratase 1, mitochondrial [Momordica charantia])

HSP 1 Score: 942.6 bits (2435), Expect = 1.4e-270
Identity = 479/505 (94.85%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG ARCA+ S RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINSRF+PKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET  PFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN ERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLK+AA
Sbjct: 421 SASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEE+FDK+VVPEKMIGPSD
Sbjct: 481 LKLGVLSEEEFDKHVVPEKMIGPSD 494

BLAST of Spg038622 vs. NCBI nr
Match: XP_022984048.1 (fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 937.2 bits (2421), Expect = 6.0e-269
Identity = 478/505 (94.65%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAALYVLSRRSSPSHLFG AR A+TS RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINS+F+PKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET  PF+
Sbjct: 241 EFSGYTTQVKYGIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFL 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN+ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDF+KYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFNKYVVPEKMIGPSD 494

BLAST of Spg038622 vs. NCBI nr
Match: XP_023525966.1 (fumarate hydratase 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 481/505 (95.25%), Postives = 484/505 (95.84%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAALYVLSRRSS S LFG AR A+   RSYSTAFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSSSQLFGMARYAT---RSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMP+PIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPDPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINSRF+PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSRFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEE  LPFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEEANLPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN ERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANSERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDFDKYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 491

BLAST of Spg038622 vs. NCBI nr
Match: XP_023528894.1 (fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 477/505 (94.46%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA+YVLSRRSSPSHLFG AR A+TS RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINS+F+PKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPF+
Sbjct: 241 EFSGYTTQVKYGIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFL 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           S SFEKNCVRGIQAN+ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SGSFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDF+KYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFNKYVVPEKMIGPSD 494

BLAST of Spg038622 vs. NCBI nr
Match: XP_004152237.1 (fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical protein Csa_014636 [Cucumis sativus])

HSP 1 Score: 934.9 bits (2415), Expect = 3.0e-268
Identity = 474/505 (93.86%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA+YVLSRRSS SHLFG AR A+TS RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRG+KFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGDKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINS+F+PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN+ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDFDK+VVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFDKHVVPEKMIGPSD 494

BLAST of Spg038622 vs. ExPASy Swiss-Prot
Match: P93033 (Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1 SV=2)

HSP 1 Score: 852.4 bits (2201), Expect = 2.5e-246
Identity = 434/503 (86.28%), Postives = 458/503 (91.05%), Query Frame = 0

Query: 3   ALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        R A TS RSYST+FREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSN 182
           VVWQTGSGTQSNMNANE           VIANRA+EILG KRGEK VHPNDHVNRSQSSN
Sbjct: 122 VVWQTGSGTQSNMNANE-----------VIANRAAEILGRKRGEKCVHPNDHVNRSQSSN 181

Query: 183 DTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEF 242
           DTFPTVMHIAAATEINSR +P LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF
Sbjct: 182 DTFPTVMHIAAATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEF 241

Query: 243 SGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTA 302
            GY TQVKYG++RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTA
Sbjct: 242 GGYATQVKYGLNRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTA 301

Query: 303 ENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIM 362
           ENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIM
Sbjct: 302 ENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIM 361

Query: 363 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASA 422
           PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASA
Sbjct: 362 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASA 421

Query: 423 SFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALK 482
           SFEKNCVRGI+AN+ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLKEAAL 
Sbjct: 422 SFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALN 481

Query: 483 LGVLSEEDFDKYVVPEKMIGPSD 506
           LGVL+ E+FD  VVPEKMIGPSD
Sbjct: 482 LGVLTAEEFDTLVVPEKMIGPSD 492

BLAST of Spg038622 vs. ExPASy Swiss-Prot
Match: Q9FI53 (Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 8.5e-242
Identity = 419/478 (87.66%), Postives = 442/478 (92.47%), Query Frame = 0

Query: 28  RSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVRTTLFYF 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE        
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE-------- 152

Query: 148 DRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFVPKLKT 207
              VIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR +P LK 
Sbjct: 153 ---VIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKN 212

Query: 208 LHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLA 267
           LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLA
Sbjct: 213 LHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLA 272

Query: 268 QGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVETSGALNTVAT 327
           QGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTAENKFEALAAHDA VETSG+LNT+AT
Sbjct: 273 QGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIAT 332

Query: 328 SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 387
           SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA
Sbjct: 333 SLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 392

Query: 388 VTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKERISKLLRESL 447
           VT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN+ERISKLL ESL
Sbjct: 393 VTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESL 452

Query: 448 MLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDFDKYVVPEKMIGPSD 506
           MLVT+LNPKIGYDNAAAVAK AHKEG TLK AA+KLGVL+ E+FD  VVPEKMIGPSD
Sbjct: 453 MLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of Spg038622 vs. ExPASy Swiss-Prot
Match: P55250 (Fumarate hydratase, mitochondrial OS=Rhizopus oryzae OX=64495 GN=FUMR PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 2.2e-189
Identity = 342/503 (67.99%), Postives = 392/503 (77.93%), Query Frame = 0

Query: 10  RSSPSHLFGFARCASTSFRSYSTA------FREERDTFGPINVPSDRLWGAQTQRSLQNF 69
           R+S +     A+  + S R +S+A      FR ERDTFG + VP+DR WGAQTQRSLQNF
Sbjct: 3   RASATRFLSQAKNMNNSPRLFSSASAALQKFRAERDTFGDLQVPADRYWGAQTQRSLQNF 62

Query: 70  EIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLV 129
           +IGG  ERMPEP+IRAFGVLKK AA VNM YGLDP +G+AI +AA EV +G L DHFPLV
Sbjct: 63  DIGGPTERMPEPLIRAFGVLKKAAATVNMTYGLDPKVGEAIQKAADEVIDGSLIDHFPLV 122

Query: 130 VWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKF-VHPNDHVNRSQSSN 189
           VWQTGSGTQ+ MN NE           VI+NRA E+LG + G K  VHPNDHVN SQSSN
Sbjct: 123 VWQTGSGTQTKMNVNE-----------VISNRAIELLGGELGSKAPVHPNDHVNMSQSSN 182

Query: 190 DTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEF 249
           DTFPT MH+AA  EI+ R +P L TL  +L +KS EF+ I+KIGRTH QDATPLTLGQEF
Sbjct: 183 DTFPTAMHVAAVVEIHGRLIPALTTLRDALQAKSAEFEHIIKIGRTHLQDATPLTLGQEF 242

Query: 250 SGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTA 309
           SGYT Q+ YGI RV  TL R+Y LAQGGTAVGTGLNT+KGFD K+A A+A  T LPF TA
Sbjct: 243 SGYTQQLTYGIARVQGTLERLYNLAQGGTAVGTGLNTRKGFDAKVAEAIASITGLPFKTA 302

Query: 310 ENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIM 369
            NKFEALAAHDA VE  GALNTVA SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIM
Sbjct: 303 PNKFEALAAHDALVEAHGALNTVACSLMKIANDIRYLGSGPRCGLGELSLPENEPGSSIM 362

Query: 370 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASA 429
           PGKVNPTQCEA+TMVCAQVMGN+ A++V GSNG FELNVFKP++   L+ S+RL+ DAS 
Sbjct: 363 PGKVNPTQCEAMTMVCAQVMGNNTAISVAGSNGQFELNVFKPVMIKNLIQSIRLISDASI 422

Query: 430 SFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALK 489
           SF KNCV GI+AN+++IS ++ ESLMLVTALNP IGYD AA  AK AHKEG+TLKEAAL 
Sbjct: 423 SFTKNCVVGIEANEKKISSIMNESLMLVTALNPHIGYDKAAKCAKKAHKEGTTLKEAALS 482

Query: 490 LGVLSEEDFDKYVVPEKMIGPSD 506
           LG L+ E+FD++V PE MI   D
Sbjct: 483 LGYLTSEEFDQWVRPEDMISAKD 494

BLAST of Spg038622 vs. ExPASy Swiss-Prot
Match: P14408 (Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2)

HSP 1 Score: 662.5 bits (1708), Expect = 3.7e-189
Identity = 339/485 (69.90%), Postives = 389/485 (80.21%), Query Frame = 0

Query: 21  RCASTSFRSYS-TAFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRA 80
           RCA    R  S  +FR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+I+A
Sbjct: 33  RCAPNVVRMASQNSFRIEYDTFGELKVPTDKYYGAQTVRSTMNFKIGGATERMPIPVIKA 92

Query: 81  FGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE 140
           FG+LK+ AA+VN EYGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NE
Sbjct: 93  FGILKRAAAEVNQEYGLDPKIASAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNE 152

Query: 141 VRTTLFYFDRQVIANRASEILGHKRG-EKFVHPNDHVNRSQSSNDTFPTVMHIAAATEIN 200
                      VI+NRA E+LG + G +K VHPNDHVN+SQSSNDTFPT MHIAAA E++
Sbjct: 153 -----------VISNRAIEMLGGELGSKKPVHPNDHVNKSQSSNDTFPTAMHIAAALEVH 212

Query: 201 SRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLC 260
              +P L+ LH +L +KS EF  ++KIGRTHTQDA PLTLGQEFSGY  QV+Y ++R+  
Sbjct: 213 QVLLPGLQKLHDALSAKSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMERIKA 272

Query: 261 TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVET 320
            +PR+Y+LA GGTAVGTGLNT+ GF  K+AA VA  T LPFVTA NKFEALAAHDA VE 
Sbjct: 273 AMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVEL 332

Query: 321 SGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVC 380
           SGA+NT A SLMKIANDIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV 
Sbjct: 333 SGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVA 392

Query: 381 AQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKER 440
           AQVMGNHVAVTVGGSNGHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQAN ER
Sbjct: 393 AQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTER 452

Query: 441 ISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDFDKYVVPE 500
           I+KL+ ESLMLVTALNP IGYD AA +AKTAHK GSTLKE A++LG L+ E FD++V P+
Sbjct: 453 INKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPK 506

Query: 501 KMIGP 504
            M+GP
Sbjct: 513 DMLGP 506

BLAST of Spg038622 vs. ExPASy Swiss-Prot
Match: Q60HF9 (Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 8.3e-189
Identity = 335/472 (70.97%), Postives = 382/472 (80.93%), Query Frame = 0

Query: 33  AFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM 92
           +FR E DTFG + VP+D+ +GAQT RS  NF+IGG  ERMP P+I+AFG+LK+ AA+VN 
Sbjct: 49  SFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQ 108

Query: 93  EYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVRTTLFYFDRQVI 152
           +YGLDP I  AIM+AA EVAEGKLNDHFPLVVWQTGSGTQ+NMN NE           VI
Sbjct: 109 DYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNE-----------VI 168

Query: 153 ANRASEILGHKRGEKF-VHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFVPKLKTLHSS 212
           +NRA E+LG + G K  VHPNDHVN+SQSSNDTFPT MHIAAA E++   +P L+ LH +
Sbjct: 169 SNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDA 228

Query: 213 LYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLAQGGT 272
           L +KS EF  I+KIGRTHTQDA PLTLGQEFSGY  QVKY + R+   +PR+Y+LA GGT
Sbjct: 229 LDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAVTRIKAAMPRIYELAAGGT 288

Query: 273 AVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVETSGALNTVATSLMK 332
           AVGTGLNT+ GF  K+AA VA  T LPFVTA NKFEALAAHDA VE SGA+NT A SLMK
Sbjct: 289 AVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMK 348

Query: 333 IANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAVTVG 392
           IANDIR LGSGPR GLGELILPENEPGSSIMPGKVNPTQCEA+TMV AQVMGNHVAVTVG
Sbjct: 349 IANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVG 408

Query: 393 GSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKERISKLLRESLMLVT 452
           GSNGHFELNVFKPM+   +LHS RLLGDAS SF +NCV GIQAN ERI+KL+ ESLMLVT
Sbjct: 409 GSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVT 468

Query: 453 ALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDFDKYVVPEKMIGP 504
           ALNP IGYD AA +AKTAHK GSTLKE A++LG L+ E FD++V P+ M+GP
Sbjct: 469 ALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGP 509

BLAST of Spg038622 vs. ExPASy TrEMBL
Match: A0A6J1DLZ0 (Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 6.9e-271
Identity = 479/505 (94.85%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA YVLSRRSSPSHLFG ARCA+ S RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAAYVLSRRSSPSHLFGMARCATASLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINSRF+PKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSRFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET  PFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN ERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLK+AA
Sbjct: 421 SASFEKNCVRGIQANTERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKDAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEE+FDK+VVPEKMIGPSD
Sbjct: 481 LKLGVLSEEEFDKHVVPEKMIGPSD 494

BLAST of Spg038622 vs. ExPASy TrEMBL
Match: A0A6J1J9G0 (Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.9e-269
Identity = 478/505 (94.65%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAALYVLSRRSSPSHLFG AR A+TS RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSPSHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINS+F+PKLKTLHSSL SKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSKFIPKLKTLHSSLNSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVL TLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET  PF+
Sbjct: 241 EFSGYTTQVKYGIDRVLRTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNFPFL 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVA+TVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN+ERISKLL ESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDF+KYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFNKYVVPEKMIGPSD 494

BLAST of Spg038622 vs. ExPASy TrEMBL
Match: A0A6J1KEJ4 (Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493132 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.4e-268
Identity = 480/505 (95.05%), Postives = 484/505 (95.84%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAALYVLSRRSS S LFG AR A+   RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSSSQLFGMARYAT---RSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMP+PIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPDPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINSRF+PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSRFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEE  LPFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEEANLPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN ERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANNERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDFDKYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 491

BLAST of Spg038622 vs. ExPASy TrEMBL
Match: A0A0A0KWM0 (Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.4e-268
Identity = 474/505 (93.86%), Postives = 486/505 (96.24%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAA+YVLSRRSS SHLFG AR A+TS RSYST+FREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAAVYVLSRRSSASHLFGMARYATTSLRSYSTSFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNM+YGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRG+KFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGDKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINS+F+PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSKFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSG+LNTVA SLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGSLNTVAASLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVA+TVGGSNGHFELNV+KPMIANALLHSLRLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVYKPMIANALLHSLRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN+ERISKLL ESLMLVTALNPKIGYDNAAAVAK AHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANRERISKLLHESLMLVTALNPKIGYDNAAAVAKKAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDFDK+VVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFDKHVVPEKMIGPSD 494

BLAST of Spg038622 vs. ExPASy TrEMBL
Match: A0A6J1GD43 (Fumarate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452856 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.4e-268
Identity = 480/505 (95.05%), Postives = 484/505 (95.84%), Query Frame = 0

Query: 1   MAALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60
           MAALYVLSRRSS S LFG AR A+   RSYSTAFREERDTFGPINVPSDRLWGAQTQRSL
Sbjct: 1   MAALYVLSRRSSSSQLFGMARYAT---RSYSTAFREERDTFGPINVPSDRLWGAQTQRSL 60

Query: 61  QNFEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120
           QNFEIGGDRERMP+PIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF
Sbjct: 61  QNFEIGGDRERMPDPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHF 120

Query: 121 PLVVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQS 180
           PLVVWQTGSGTQSNMNANE           VIANRASEILGHKRGEKFVHPNDHVNRSQS
Sbjct: 121 PLVVWQTGSGTQSNMNANE-----------VIANRASEILGHKRGEKFVHPNDHVNRSQS 180

Query: 181 SNDTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240
           SNDTFPTVMHIAAATEINSRF+PKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ
Sbjct: 181 SNDTFPTVMHIAAATEINSRFIPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQ 240

Query: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFV 300
           EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEE  LPFV
Sbjct: 241 EFSGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEEANLPFV 300

Query: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360
           TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS
Sbjct: 301 TAENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSS 360

Query: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDA 420
           IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHS+RLLGDA
Sbjct: 361 IMPGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSVRLLGDA 420

Query: 421 SASFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480
           SASFEKNCVRGIQAN ERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA
Sbjct: 421 SASFEKNCVRGIQANNERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAA 480

Query: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 506
           LKLGVLSEEDFDKYVVPEKMIGPSD
Sbjct: 481 LKLGVLSEEDFDKYVVPEKMIGPSD 491

BLAST of Spg038622 vs. TAIR 10
Match: AT2G47510.1 (fumarase 1 )

HSP 1 Score: 852.4 bits (2201), Expect = 1.8e-247
Identity = 434/503 (86.28%), Postives = 458/503 (91.05%), Query Frame = 0

Query: 3   ALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        R A TS RSYST+FREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSN 182
           VVWQTGSGTQSNMNANE           VIANRA+EILG KRGEK VHPNDHVNRSQSSN
Sbjct: 122 VVWQTGSGTQSNMNANE-----------VIANRAAEILGRKRGEKCVHPNDHVNRSQSSN 181

Query: 183 DTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEF 242
           DTFPTVMHIAAATEINSR +P LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF
Sbjct: 182 DTFPTVMHIAAATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEF 241

Query: 243 SGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTA 302
            GY TQVKYG++RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTA
Sbjct: 242 GGYATQVKYGLNRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTA 301

Query: 303 ENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIM 362
           ENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIM
Sbjct: 302 ENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIM 361

Query: 363 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASA 422
           PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASA
Sbjct: 362 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASA 421

Query: 423 SFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALK 482
           SFEKNCVRGI+AN+ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLKEAAL 
Sbjct: 422 SFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALN 481

Query: 483 LGVLSEEDFDKYVVPEKMIGPSD 506
           LGVL+ E+FD  VVPEKMIGPSD
Sbjct: 482 LGVLTAEEFDTLVVPEKMIGPSD 492

BLAST of Spg038622 vs. TAIR 10
Match: AT2G47510.2 (fumarase 1 )

HSP 1 Score: 852.4 bits (2201), Expect = 1.8e-247
Identity = 434/503 (86.28%), Postives = 458/503 (91.05%), Query Frame = 0

Query: 3   ALYVLSRRSSPSHLFGFARCASTSFRSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQN 62
           ++YV SRR S        R A TS RSYST+FREERDTFGPI VPSD+LWGAQTQRSLQN
Sbjct: 2   SIYVASRRLSGGTTVTALRYA-TSLRSYSTSFREERDTFGPIQVPSDKLWGAQTQRSLQN 61

Query: 63  FEIGGDRERMPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 122
           FEIGG+RERMPEPI+RAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL
Sbjct: 62  FEIGGERERMPEPIVRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPL 121

Query: 123 VVWQTGSGTQSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSN 182
           VVWQTGSGTQSNMNANE           VIANRA+EILG KRGEK VHPNDHVNRSQSSN
Sbjct: 122 VVWQTGSGTQSNMNANE-----------VIANRAAEILGRKRGEKCVHPNDHVNRSQSSN 181

Query: 183 DTFPTVMHIAAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEF 242
           DTFPTVMHIAAATEINSR +P LKTLHS+L SKS EFKDIVKIGRTHTQDATPLTLGQEF
Sbjct: 182 DTFPTVMHIAAATEINSRLIPSLKTLHSTLESKSFEFKDIVKIGRTHTQDATPLTLGQEF 241

Query: 243 SGYTTQVKYGIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTA 302
            GY TQVKYG++RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTA
Sbjct: 242 GGYATQVKYGLNRVTCTLPRLYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTA 301

Query: 303 ENKFEALAAHDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIM 362
           ENKFEALAAHDA VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL+LPENEPGSSIM
Sbjct: 302 ENKFEALAAHDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIM 361

Query: 363 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASA 422
           PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKP+IA+ALLHS+RL+ DASA
Sbjct: 362 PGKVNPTQCEALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPVIASALLHSVRLIADASA 421

Query: 423 SFEKNCVRGIQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALK 482
           SFEKNCVRGI+AN+ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLKEAAL 
Sbjct: 422 SFEKNCVRGIEANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKKAHKEGCTLKEAALN 481

Query: 483 LGVLSEEDFDKYVVPEKMIGPSD 506
           LGVL+ E+FD  VVPEKMIGPSD
Sbjct: 482 LGVLTAEEFDTLVVPEKMIGPSD 492

BLAST of Spg038622 vs. TAIR 10
Match: AT5G50950.2 (FUMARASE 2 )

HSP 1 Score: 837.4 bits (2162), Expect = 6.0e-243
Identity = 419/478 (87.66%), Postives = 442/478 (92.47%), Query Frame = 0

Query: 28  RSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVRTTLFYF 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE        
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE-------- 152

Query: 148 DRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFVPKLKT 207
              VIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR +P LK 
Sbjct: 153 ---VIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKN 212

Query: 208 LHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLA 267
           LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLA
Sbjct: 213 LHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLA 272

Query: 268 QGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVETSGALNTVAT 327
           QGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTAENKFEALAAHDA VETSG+LNT+AT
Sbjct: 273 QGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIAT 332

Query: 328 SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 387
           SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA
Sbjct: 333 SLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 392

Query: 388 VTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKERISKLLRESL 447
           VT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN+ERISKLL ESL
Sbjct: 393 VTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESL 452

Query: 448 MLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDFDKYVVPEKMIGPSD 506
           MLVT+LNPKIGYDNAAAVAK AHKEG TLK AA+KLGVL+ E+FD  VVPEKMIGPSD
Sbjct: 453 MLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEFDTLVVPEKMIGPSD 499

BLAST of Spg038622 vs. TAIR 10
Match: AT5G50950.1 (FUMARASE 2 )

HSP 1 Score: 796.2 bits (2055), Expect = 1.5e-230
Identity = 398/450 (88.44%), Postives = 418/450 (92.89%), Query Frame = 0

Query: 28  RSYSTAFREERDTFGPINVPSDRLWGAQTQRSLQNFEIGGDRERMPEPIIRAFGVLKKCA 87
           RSYST FREERDTFGPI VPSD+LWGAQTQRSLQNFEIGGDRERMPEPI+RAFGVLKKCA
Sbjct: 33  RSYSTPFREERDTFGPIQVPSDKLWGAQTQRSLQNFEIGGDRERMPEPIVRAFGVLKKCA 92

Query: 88  AKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANEVRTTLFYF 147
           AKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE        
Sbjct: 93  AKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGTQSNMNANE-------- 152

Query: 148 DRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHIAAATEINSRFVPKLKT 207
              VIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHIAAATEI SR +P LK 
Sbjct: 153 ---VIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHIAAATEITSRLIPSLKN 212

Query: 208 LHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKYGIDRVLCTLPRMYQLA 267
           LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+YG++RV CTLPR+YQLA
Sbjct: 213 LHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLPRIYQLA 272

Query: 268 QGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAAHDAFVETSGALNTVAT 327
           QGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTAENKFEALAAHDA VETSG+LNT+AT
Sbjct: 273 QGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAAHDACVETSGSLNTIAT 332

Query: 328 SLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 387
           SLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA
Sbjct: 333 SLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 392

Query: 388 VTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRGIQANKERISKLLRESL 447
           VT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRGI+AN+ERISKLL ESL
Sbjct: 393 VTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESL 452

Query: 448 MLVTALNPKIGYDNAAAVAKTAHKEGSTLK 478
           MLVT+LNPKIGYDNAAAVAK AHKEG TLK
Sbjct: 453 MLVTSLNPKIGYDNAAAVAKRAHKEGCTLK 471

BLAST of Spg038622 vs. TAIR 10
Match: AT5G50950.3 (FUMARASE 2 )

HSP 1 Score: 752.3 bits (1941), Expect = 2.5e-217
Identity = 378/434 (87.10%), Postives = 400/434 (92.17%), Query Frame = 0

Query: 72  MPEPIIRAFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNDHFPLVVWQTGSGT 131
           MPEPI+RAFGVLKKCAAKVNMEYGLDP IG+AIM+AAQEVAEGKLNDHFPLVVWQTGSGT
Sbjct: 1   MPEPIVRAFGVLKKCAAKVNMEYGLDPMIGEAIMEAAQEVAEGKLNDHFPLVVWQTGSGT 60

Query: 132 QSNMNANEVRTTLFYFDRQVIANRASEILGHKRGEKFVHPNDHVNRSQSSNDTFPTVMHI 191
           QSNMNANE           VIANRA+EILGHKRGEK VHPNDHVNRSQSSNDTFPTVMHI
Sbjct: 61  QSNMNANE-----------VIANRAAEILGHKRGEKIVHPNDHVNRSQSSNDTFPTVMHI 120

Query: 192 AAATEINSRFVPKLKTLHSSLYSKSIEFKDIVKIGRTHTQDATPLTLGQEFSGYTTQVKY 251
           AAATEI SR +P LK LHSSL SKS EFKDIVKIGRTHTQDATPLTLGQEF GY TQV+Y
Sbjct: 121 AAATEITSRLIPSLKNLHSSLESKSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEY 180

Query: 252 GIDRVLCTLPRMYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETKLPFVTAENKFEALAA 311
           G++RV CTLPR+YQLAQGGTAVGTGLNTKKGFDVKIAAAVAEET LPFVTAENKFEALAA
Sbjct: 181 GLNRVACTLPRIYQLAQGGTAVGTGLNTKKGFDVKIAAAVAEETNLPFVTAENKFEALAA 240

Query: 312 HDAFVETSGALNTVATSLMKIANDIRLLGSGPRCGLGELILPENEPGSSIMPGKVNPTQC 371
           HDA VETSG+LNT+ATSLMKIANDIR LGSGPRCGLGEL LPENEPGSSIMPGKVNPTQC
Sbjct: 241 HDACVETSGSLNTIATSLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQC 300

Query: 372 EALTMVCAQVMGNHVAVTVGGSNGHFELNVFKPMIANALLHSLRLLGDASASFEKNCVRG 431
           EALTMVCAQVMGNHVAVT+GGSNGHFELNVFKP+IA+ALLHS+RL+ DASASFEKNCVRG
Sbjct: 301 EALTMVCAQVMGNHVAVTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRG 360

Query: 432 IQANKERISKLLRESLMLVTALNPKIGYDNAAAVAKTAHKEGSTLKEAALKLGVLSEEDF 491
           I+AN+ERISKLL ESLMLVT+LNPKIGYDNAAAVAK AHKEG TLK AA+KLGVL+ E+F
Sbjct: 361 IEANRERISKLLHESLMLVTSLNPKIGYDNAAAVAKRAHKEGCTLKHAAMKLGVLTSEEF 420

Query: 492 DKYVVPEKMIGPSD 506
           D  VVPEKMIGPSD
Sbjct: 421 DTLVVPEKMIGPSD 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155265.11.4e-27094.85fumarate hydratase 1, mitochondrial [Momordica charantia][more]
XP_022984048.16.0e-26994.65fumarate hydratase 1, mitochondrial-like isoform X2 [Cucurbita maxima][more]
XP_023525966.11.3e-26895.25fumarate hydratase 1, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_023528894.11.3e-26894.46fumarate hydratase 1, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_004152237.13.0e-26893.86fumarate hydratase 1, mitochondrial [Cucumis sativus] >KGN52832.1 hypothetical p... [more]
Match NameE-valueIdentityDescription
P930332.5e-24686.28Fumarate hydratase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FUM1 PE=1... [more]
Q9FI538.5e-24287.66Fumarate hydratase 2 OS=Arabidopsis thaliana OX=3702 GN=FUM2 PE=1 SV=1[more]
P552502.2e-18967.99Fumarate hydratase, mitochondrial OS=Rhizopus oryzae OX=64495 GN=FUMR PE=3 SV=1[more]
P144083.7e-18969.90Fumarate hydratase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Fh PE=1 SV=2[more]
Q60HF98.3e-18970.97Fumarate hydratase, mitochondrial OS=Macaca fascicularis OX=9541 GN=FH PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DLZ06.9e-27194.85Fumarate hydratase OS=Momordica charantia OX=3673 GN=LOC111022407 PE=3 SV=1[more]
A0A6J1J9G02.9e-26994.65Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111482474 PE=3 SV=1[more]
A0A6J1KEJ41.4e-26895.05Fumarate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493132 PE=3 SV=1[more]
A0A0A0KWM01.4e-26893.86Fumarate hydratase OS=Cucumis sativus OX=3659 GN=Csa_4G001990 PE=3 SV=1[more]
A0A6J1GD431.4e-26895.05Fumarate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452856 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47510.11.8e-24786.28fumarase 1 [more]
AT2G47510.21.8e-24786.28fumarase 1 [more]
AT5G50950.26.0e-24387.66FUMARASE 2 [more]
AT5G50950.11.5e-23088.44FUMARASE 2 [more]
AT5G50950.32.5e-21787.10FUMARASE 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 358..374
score: 66.75
coord: 175..193
score: 38.81
coord: 312..339
score: 38.17
coord: 221..239
score: 55.26
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 181..447
e-value: 2.9E-106
score: 356.6
NoneNo IPR availableGENE3D1.10.40.30coord: 448..505
e-value: 9.1E-28
score: 98.3
NoneNo IPR availablePANTHERPTHR11444:SF24FUMARATE HYDRATASE, MITOCHONDRIALcoord: 17..505
IPR018951Fumarase C, C-terminalPFAMPF10415FumaraseC_Ccoord: 449..502
e-value: 1.1E-24
score: 86.6
IPR005677Fumarate hydratase, class IITIGRFAMTIGR00979TIGR00979coord: 34..502
e-value: 1.9E-224
score: 743.5
IPR005677Fumarate hydratase, class IIPANTHERPTHR11444ASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASEcoord: 17..505
IPR005677Fumarate hydratase, class IIHAMAPMF_00743FumaraseCcoord: 34..503
score: 87.342262
IPR005677Fumarate hydratase, class IICDDcd01362Fumarase_classIIcoord: 35..500
e-value: 0.0
score: 894.549
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 35..180
e-value: 3.4E-58
score: 197.7
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 42..383
e-value: 5.4E-113
score: 377.5
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 358..367
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 34..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg038622.1Spg038622.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006106 fumarate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion
cellular_component GO:0045239 tricarboxylic acid cycle enzyme complex
molecular_function GO:0004333 fumarate hydratase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016829 lyase activity