Spg037255 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg037255
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold13: 15001846 .. 15003500 (+)
RNA-Seq ExpressionSpg037255
SyntenySpg037255
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTTTCTCTTATTTGGTCAGATTCAGAGGCCATAGTTCTGTCTATTTGGAGCCTCTTACCTTTTTTTTAGGGATATCTCTTTCAATTTTGCTCCTAGATCTTGTAATAGGGTTGCCCATGAGATTGCTCGATATGGGAAATCTGTAAATAGCCATGACGTTTGGGTCTCTAATTTCTCAAACTGGATGTGTATGTGGGTCGAGTATGACCGTTCATTTTTAGCCCATGTGGCGTTATAATATGAGGTATTATTTTTCAAAAAAAAAAAAAAAAAAAAAAGCTTAAGGTTCCTTAAAGAGTGAAAGTTTTCCTCTAGACTTTTGCTTACAAGAATTCAAATTCTCGTTGATCACTTCTTCTTTTTTTTTTATAGAAAACCAGCTTTCATGGAGAAAACATGAAAGAATACAAGGGCATACAAAAAGACCAAGCCCATAGAAGGGAGTCCTCTTAAAGGAAGGGGCTCCAATCCAATAAGAAAAGACCTAAAGGATAATTACAAAATAAATTTGTAACCGAGGCCCAAAAGGAAGCATGGAATCTGGATAAAGACCATACATCGCCCGGGCTTCTAACTATCCCCCTAAACACTCTGTTATTTCTCTCCCCCCATAAGTTCCATAAGAGCGCACACATGTCGGCCTACCATAAAAATCTTCCTTTGCCATGGAGGGTCAGATTGAGGAGGAACTCTTAACACTCTCAGATCTAGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAATCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGGTATTAACCCTACCATGGAGAACTTTCCAGACAAAAATCCTTACCTTCTTCGACACTTTCACCTTCCACATGAAAGAAAAGATAGACTTGCTATAGGGAGGATGATTCAACAAAGAAGTGAAGTAGGATTTACATGAGAACCCGACAGAAGGATCAGGGCTTCAACAACGAAAATCTCTCCTCCCCAACCAAAAGATAGACTCGAATTTTTGGAATGCTCCTTGACTATATCTTCGAGCTCAAACTCTCGAAGATATAGTTGTTGATCACCCAAGTCTTTTCAAATTGTGGTATCAAGTTAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAATGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

mRNA sequence

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTTTCTCTTATTTGGGTTGCCCATGAGATTGCTCGATATGGGAAATCTGTAAATAGCCATGACGTTTGGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAATCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAATGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

Coding sequence (CDS)

ATGCGGATCAAAAGTAATGAGGATTCTTCTTCTTTGGACAGAAATCGACGGTGGTCTCCTCCGAATGGCGTATTTTGGAAGCTTAACTCTGATGCTGCATGTTCGAAAGAGGTCTCGATGACAAGTCTTGGGATGATCTGCAGAAATTTTGAGGGCAAAGTTACGGTAGCTGCGTCAAAGGCTTTGGACGTGAGGTTGGAGGCCCATATTGCTGAATTATGGGCCATCCTTGAAGGTTTAAAGCTGGTTATTTCATTGGGCTGCACTATGTTAGTTGTGGAGTCCGATTGTCAACTTGCTATCAATTTCATCAATAAGGTTTCTCTTATTTGGGTTGCCCATGAGATTGCTCGATATGGGAAATCTGTAAATAGCCATGACGTTTGGGTAGTTGATCCGTTGCTGCCCAAACGCATCAAAGAAAAGGTCCCAACGGGAGCGAACAAAATCACAGCTCCAAAGAATATGATCCAAATCTTCCTCCACCTTCTGACAAAGGATGGAACAAAAGGGGCCAAACAAATGGGCATTCCTTCTCAAGAGCCTACCAAAGTTGGTCCAGACTTTTGCAAAATCTTTTGGAATGTCTACGTGCACAATGATGTGGATATTGTCCTTCCTGAACTCTCATTAAGTGAGTGGATCAAGGGAAAGGCTAGGTTCACGTGGAAGAGTGCGGCTAGAACTATTCCTTTGGTCTCTTTGGATTGA

Protein sequence

MRIKSNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFINKVSLIWVAHEIARYGKSVNSHDVWVVDPLLPKRIKEKVPTGANKITAPKNMIQIFLHLLTKDGTKGAKQMGIPSQEPTKVGPDFCKIFWNVYVHNDVDIVLPELSLSEWIKGKARFTWKSAARTIPLVSLD
Homology
BLAST of Spg037255 vs. NCBI nr
Match: XP_040996092.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-10
Identity = 42/92 (45.65%), Postives = 58/92 (63.04%), Query Frame = 0

Query: 17  RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
           RWSPP+    KLN D A   E++ TSLG I RN  G V +A S+A  V+LE    E  A+
Sbjct: 317 RWSPPSQDSLKLNIDGALFFELNKTSLGAILRNHVGDVLMAVSRAKSVQLELEQVEAAAL 376

Query: 77  LEGLKLVISLGCTMLVVESDCQLAINFINKVS 109
           L GL+L +++G + LV+ESDC   +  +NKV+
Sbjct: 377 LRGLQLCLNIGISKLVIESDCLFLVEEVNKVA 408

BLAST of Spg037255 vs. NCBI nr
Match: OMO72757.1 (hypothetical protein CCACVL1_17615 [Corchorus capsularis])

HSP 1 Score: 70.9 bits (172), Expect = 1.7e-08
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V  +  K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFIN 106
              GL+L  S G    V+ESDC LAI  IN
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAITAIN 1117

BLAST of Spg037255 vs. NCBI nr
Match: XP_024033533.1 (uncharacterized protein LOC112095662 [Citrus clementina])

HSP 1 Score: 70.1 bits (170), Expect = 2.9e-08
Identity = 43/134 (32.09%), Postives = 66/134 (49.25%), Query Frame = 0

Query: 1   MRIKSNEDSSSLDRN--RRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAA 60
           M+ K  +  SS+     +RW+PP     K+N DAA   E  M  LG + RN +G+V VAA
Sbjct: 86  MQFKEQDSPSSIKEKEAQRWNPPPSSKLKINVDAAVHAESQMAGLGAVIRNSQGQVVVAA 145

Query: 61  SKALDVRLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFI-----NKVSLIWVA 120
            K+++ + +  I E  A+  GL++      T ++VE+DC +  N       NK  + W  
Sbjct: 146 VKSINFQGDVSIVEAKAVQWGLEVASKASFTNVIVETDCSMVANLANNKVSNKTEIWWTI 205

Query: 121 HEIARYGKSVNSHD 128
            EI    +   S D
Sbjct: 206 AEIQSSRQDFQSID 219

BLAST of Spg037255 vs. NCBI nr
Match: XP_015388012.1 (uncharacterized protein LOC107177940 [Citrus sinensis])

HSP 1 Score: 69.7 bits (169), Expect = 3.8e-08
Identity = 43/134 (32.09%), Postives = 67/134 (50.00%), Query Frame = 0

Query: 1   MRIKSNEDSSSLDRN--RRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAA 60
           M+ K  +  SS+     +RW+PP     K+N DAA   E  M  LG + RN +G+V VAA
Sbjct: 72  MQFKEQDSPSSIKEKEAQRWNPPPSSKLKINVDAAVHAESQMAGLGAVIRNSQGQVVVAA 131

Query: 61  SKALDVRLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFI-----NKVSLIWVA 120
            K+++ + +  IAE  A+  GL++      T ++VE+DC +  +       NK  + W  
Sbjct: 132 VKSINFQGDVSIAEAKAVQWGLEVASKASFTNVIVETDCSMVADLANNKVSNKTEIWWTI 191

Query: 121 HEIARYGKSVNSHD 128
            EI    +   S D
Sbjct: 192 AEIQSSRQDFQSID 205

BLAST of Spg037255 vs. NCBI nr
Match: XP_015386618.1 (uncharacterized protein LOC107177388 [Citrus sinensis])

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-07
Identity = 39/114 (34.21%), Postives = 62/114 (54.39%), Query Frame = 0

Query: 8   DSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLE 67
           +SS++++ ++W PP   + K+N DAA   +     LG++ RN +G V  AA K      +
Sbjct: 97  ESSNMEKPKQWKPPLENWCKVNVDAAIDHQSQRAGLGVVIRNDKGDVVAAAIKPSSFNGD 156

Query: 68  AHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFIN-----KVSLIWVAHEI 117
              AE+ AI  G+++    G T +++ESDCQ AI+  N     K  + WV  EI
Sbjct: 157 VPFAEVEAIEWGMQMAKRAGITTVILESDCQAAIDLANNKKGSKSEIFWVISEI 210

BLAST of Spg037255 vs. ExPASy TrEMBL
Match: A0A1R3HQR7 (Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 8.3e-09
Identity = 39/89 (43.82%), Postives = 53/89 (59.55%), Query Frame = 0

Query: 17   RWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAI 76
            RWSPP    WK+N+DAA  K++ +  LG + R+FEG+V  +  K +    +   A L+AI
Sbjct: 1029 RWSPPAVGSWKINTDAAFDKDLGVAGLGAVLRDFEGRVLCSIIKKMHFIQDPLFAVLYAI 1088

Query: 77   LEGLKLVISLGCTMLVVESDCQLAINFIN 106
              GL+L  S G    V+ESDC LAI  IN
Sbjct: 1089 QLGLQLARSEGFLTCVIESDCLLAITAIN 1117

BLAST of Spg037255 vs. ExPASy TrEMBL
Match: A0A1S8ABJ9 (Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 9.1e-08
Identity = 38/89 (42.70%), Postives = 51/89 (57.30%), Query Frame = 0

Query: 18  WSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAELWAIL 77
           W PP+   +KLN+DAA  KE  +T LG I RN +G V  A +       +  IAE  AIL
Sbjct: 148 WHPPDPPRYKLNTDAALDKEGGVTGLGCIIRNHQGAVMAAVTSKKPYFGDVEIAEALAIL 207

Query: 78  EGLKLVISLGCTMLVVESDCQLAINFINK 107
           EGLKL   +  +  ++ESD +  +NFI K
Sbjct: 208 EGLKLAAEVALSPHLIESDSKNVVNFILK 236

BLAST of Spg037255 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 9.1e-08
Identity = 45/100 (45.00%), Postives = 55/100 (55.00%), Query Frame = 0

Query: 5   SNEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDV 64
           S + SS    + RW PP     K+NSDAAC    + T LG+I R+  G + VA S  L +
Sbjct: 102 SPQQSSVPPPSCRWLPPRDSAMKVNSDAACRS--TSTGLGLIIRDHFGVLLVAKSMFLPM 161

Query: 65  RLEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFI 105
            L    AE+  ILE LKL  S   T LVVESDCQ AI  +
Sbjct: 162 PLNPLFAEIRGILEALKLAASRSYTRLVVESDCQEAIRLV 199

BLAST of Spg037255 vs. ExPASy TrEMBL
Match: A0A1R3GKQ5 (Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.7e-07
Identity = 36/99 (36.36%), Postives = 59/99 (59.60%), Query Frame = 0

Query: 14   RNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVRLEAHIAEL 73
            R   W  P+   WK+N+DA+ S       LG++ R++EGK+ V+ ++ L    ++  AE+
Sbjct: 1461 RELEWIAPSPGTWKINTDASFSSSTGDAGLGVVIRDYEGKILVSGARILFFVADSLHAEV 1520

Query: 74   WAILEGLKLVISLGCTMLVVESDCQLAINFIN-KVSLIW 112
             AIL G +L +  G T  ++ESD  LAI+ IN K +++W
Sbjct: 1521 HAILFGFELALEHGITRCIIESDSLLAIHEINKKETVLW 1559

BLAST of Spg037255 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 4.5e-07
Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 0

Query: 6   NEDSSSLDRNRRWSPPNGVFWKLNSDAACSKEVSMTSLGMICRNFEGKVTVAASKALDVR 65
           N + +  D N  W+PP+  + K+N D AC    S T +G++C+N +G++ VA  + LDV 
Sbjct: 117 NSNKAGKDVN-YWAPPSPDWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVF 176

Query: 66  LEAHIAELWAILEGLKLVISLGCTMLVVESDCQLAINFINK 107
            E    E  AILEGL L   L    + V+SD Q +IN I++
Sbjct: 177 YEPLQVETLAILEGLWLTQCLDLIFVQVDSDSQNSINLISQ 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_040996092.11.4e-1045.65LOW QUALITY PROTEIN: uncharacterized protein LOC121242268 [Juglans microcarpa x ... [more]
OMO72757.11.7e-0843.82hypothetical protein CCACVL1_17615 [Corchorus capsularis][more]
XP_024033533.12.9e-0832.09uncharacterized protein LOC112095662 [Citrus clementina][more]
XP_015388012.13.8e-0832.09uncharacterized protein LOC107177940 [Citrus sinensis][more]
XP_015386618.11.4e-0734.21uncharacterized protein LOC107177388 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1R3HQR78.3e-0943.82Ribonuclease H OS=Corchorus capsularis OX=210143 GN=CCACVL1_17615 PE=4 SV=1[more]
A0A1S8ABJ99.1e-0842.70Ribonuclease H-like superfamily protein (Fragment) OS=Citrus limon OX=2708 PE=4 ... [more]
A0A6J1BQ499.1e-0845.00uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A1R3GKQ52.7e-0736.36Reverse transcriptase OS=Corchorus capsularis OX=210143 GN=CCACVL1_25420 PE=4 SV... [more]
A0A6J1DJL64.5e-0738.61uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 25..149
e-value: 1.3E-10
score: 43.5
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 29..107
e-value: 3.5E-14
score: 52.7
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 13..114
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 28..106
e-value: 8.60433E-14
score: 63.8724
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 26..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg037255.1Spg037255.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity