Spg036456 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036456
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Epoxide hydrolase-like
Location	scaffold5: 41826238 .. 41828326 (-)
RNA-Seq Expression	Spg036456
Synteny	Spg036456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAAACTTAAACCAGTTCTTTGTTACCATTACCAGAGGGCAGAGGAGCTGTTCAACGTGGCTCCCACAACAATTCTTCTCTCTTTCAAATCTCCAACATAAACCAAACAAGTCAAAGAATCTCTCTCTCTCGATCATGGATCAAATTCAACACAAGTTCATTAAAATCAGAGAATCGAAGCTTCACTTTGCTGAAATTGGAACTGGTTTCGCCTCTGTTTTTTTTTTTCACAGATCAATTGGGTTTTTCACATGATCTTCAATCAATTCACCATTTCTTCTTTGCAGGGTCAGCAGCCGCCATCGTGTTCATTCATGGGTTCCCTGAGATTTGGTATTCGTGGCGGCACCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCACCGGATTTAAGAGGCTACGGTCTTTCGGATCCACACCCACGGCCTGAAAACGCAACGTTCGAGGACTTTGCTGAAGACATTCTTGGAATCCTCGATTTTCTTCAGATTTCCAAGGCTTTTCTGGTCGGAAAGGATTTTGGGTCTTGGGTTGCTTACTTGTTTTCTCTGTTGTTTCCGGCGAGGGTCGCCGGAATCGTGTCTCTCGGTGTCCCCTATTTGCCCCCGAATCCGACACGGTACCGGGACCTCCCTGAGGGTTTCTATATTTTCCGGTGGAAGGTATTTTCCGGCCGGTCTTATTTTCCGGTGGATCTCCCTGAGGGTTTCTGAGTTTCTTTTCAACCTTTTTAATTTTGTATTTAAGAAATCAGCTCATTATCATTTTTTTAAAAAAAAAAAAAAAATTATATTTGTTTTCTGACACATTTGAATTTTTAGCAAAAATTTAAAAAAAAAAAAAAAAACAGTTTTTAAAAACATTTTTTTTAGTTTCTAAAACTTAACTTGTTTTTTTTTTTTTTAAGAAGAAAAAATTTATAATTATTTCGCCACTTGGGCAGAAACGGGTTGGTCATTCTCGACCACAAAAACAATCCACTCAGGGATGACGTATGTCCAGCTCTCATTTAACACATTGGAAGAAAATAAATAACAAAAAAAAAAAAAAAAAACTTATAGGTGAAAGTAGTGTTTGTTTAATTTTCAAAATCAGTGGTTATCAATCACGATCTGATTAAAGTGTTTTTTTTTTTATGTAATAGGTTTATTTCCGATGAGGTAAATAATATGCTTGTCATATGCCATTTAAACTAATAACATCGATGATCGAATCTATTTGATGATTAGAGATCTAAAAATTTATTACAAACTTTTTAAAAATACATTGACTAAATAGATACCACTAAAAAATTACATGAACTAAATAGACACTGTTCTAGAACTTGAAGATCAAATTTTTATAGTTCTAAAATGATTGAGTTTCTTTATTGCTCTTTCCATTATTTTCCAGGAAAGTGGAAGGGCTGAAGCAGACTTTGGGAGATTTGATGTGAAGACTGTAGTGAGTAGAATATACACTCTCTTCTCCAGAAGTGAAATACCCATTGCTGAAAAAGATCAAGAGATCATGGACATGGTGGACGAATCAACCCCTCGACCGCCATGGCTGACCGACGAAGATCTCGCCGCCTATGCAGCTTTATATGAACATTCTGGGTTTGGATCTACCTTGCAAGTTCCTTACAGGTATGGAGTTTTTCTCATCAATCTAAATTCTGAACAAAAATTGGTACCATGAACTGAGAAACAAAGTAAAATTGACTGAATTTTAGGAGAAAACATAAGGAGTTGGGCATGAGCAATCCAAGGGTTGAAGTTCCAGTTTTGTTGATCATTGGTGGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATTTAAAGAGTGACAAATTAAGGGAATTTGTGGCTGATTTGGAGGTTGCAGATTTGGCTGATGGAACCCATTTCATGCAGGAGCAGTTCCCAGCCCAAGTCAATCAGTTTCTCATTAGCTTTCTAAGTAAACATAATTATTGAAGTATCGAATATTTGGCTGCATACCGATAAATATCTCTCTACGCACCACTATTAAAATGTTGTATATAGCCAAGTATTCTTCATATTTGAATAACTAAAACTAATTATAACGTGAG

mRNA sequence

ATGGATCAAATTCAACACAAGTTCATTAAAATCAGAGAATCGAAGCTTCACTTTGCTGAAATTGGAACTGGGTCAGCAGCCGCCATCGTGTTCATTCATGGGTTCCCTGAGATTTGGTATTCGTGGCGGCACCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCACCGGATTTAAGAGGCTACGGTCTTTCGGATCCACACCCACGGCCTGAAAACGCAACGTTCGAGGACTTTGCTGAAGACATTCTTGGAATCCTCGATTTTCTTCAGATTTCCAAGGCTTTTCTGGTCGGAAAGGATTTTGGGTCTTGGGTTGCTTACTTGTTTTCTCTGTTGTTTCCGGCGAGGGTCGCCGGAATCGTGTCTCTCGGTGTCCCCTATTTGCCCCCGAATCCGACACGGTACCGGGACCTCCCTGAGGGTTTCTATATTTTCCGGTGGAAGGAAAGTGGAAGGGCTGAAGCAGACTTTGGGAGATTTGATGTGAAGACTGTAGTGAGTAGAATATACACTCTCTTCTCCAGAAGTGAAATACCCATTGCTGAAAAAGATCAAGAGATCATGGACATGGTGGACGAATCAACCCCTCGACCGCCATGGCTGACCGACGAAGATCTCGCCGCCTATGCAGCTTTATATGAACATTCTGGGTTTGGATCTACCTTGCAAGTTCCTTACAGGAGAAAACATAAGGAGTTGGGCATGAGCAATCCAAGGGTTGAAGTTCCAGTTTTGTTGATCATTGGTGGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATTTAAAGAGTGACAAATTAAGGGAATTTGTGGCTGATTTGGAGGTTGCAGATTTGGCTGATGGAACCCATTTCATGCAGGAGCAGTTCCCAGCCCAAGTCAATCAGTTTCTCATTAGCTTTCTAAGTAAACATAATTATTGA

Coding sequence (CDS)

ATGGATCAAATTCAACACAAGTTCATTAAAATCAGAGAATCGAAGCTTCACTTTGCTGAAATTGGAACTGGGTCAGCAGCCGCCATCGTGTTCATTCATGGGTTCCCTGAGATTTGGTATTCGTGGCGGCACCAGATGGTTGCGGCGGCGGCCGCCGGCTATCGTGCAATTTCACCGGATTTAAGAGGCTACGGTCTTTCGGATCCACACCCACGGCCTGAAAACGCAACGTTCGAGGACTTTGCTGAAGACATTCTTGGAATCCTCGATTTTCTTCAGATTTCCAAGGCTTTTCTGGTCGGAAAGGATTTTGGGTCTTGGGTTGCTTACTTGTTTTCTCTGTTGTTTCCGGCGAGGGTCGCCGGAATCGTGTCTCTCGGTGTCCCCTATTTGCCCCCGAATCCGACACGGTACCGGGACCTCCCTGAGGGTTTCTATATTTTCCGGTGGAAGGAAAGTGGAAGGGCTGAAGCAGACTTTGGGAGATTTGATGTGAAGACTGTAGTGAGTAGAATATACACTCTCTTCTCCAGAAGTGAAATACCCATTGCTGAAAAAGATCAAGAGATCATGGACATGGTGGACGAATCAACCCCTCGACCGCCATGGCTGACCGACGAAGATCTCGCCGCCTATGCAGCTTTATATGAACATTCTGGGTTTGGATCTACCTTGCAAGTTCCTTACAGGAGAAAACATAAGGAGTTGGGCATGAGCAATCCAAGGGTTGAAGTTCCAGTTTTGTTGATCATTGGTGGAAAGGACTATTTTCTGAAATTTCCAGGGATTGAAGACTATTTAAAGAGTGACAAATTAAGGGAATTTGTGGCTGATTTGGAGGTTGCAGATTTGGCTGATGGAACCCATTTCATGCAGGAGCAGTTCCCAGCCCAAGTCAATCAGTTTCTCATTAGCTTTCTAAGTAAACATAATTATTGA

Protein sequence

MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMSNPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQVNQFLISFLSKHNY

Homology

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BLAST of Spg036456 vs. NCBI nr
Match: XP_038905360.1 (bifunctional epoxide hydrolase 2-like [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 266/311 (85.53%), Postives = 286/311 (91.96%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQI H F+ I+E KLH AEIGTGS   +VF+HGFPEIWYSWRHQMVAAAAAGYRAI PD
Sbjct: 1   MDQIHHNFVTIKEVKLHIAEIGTGS-TVVVFLHGFPEIWYSWRHQMVAAAAAGYRAICPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYG SDPHP P+NA+FEDF EDI+GILDFL I KAFLVGKDFGSWV YLFSL +PARV
Sbjct: 61  LRGYGFSDPHPHPQNASFEDFVEDIVGILDFLHIPKAFLVGKDFGSWVVYLFSLFYPARV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
           AG+VSLGVP+LPPNP RYRDLPEGFYIFRWKESGRAEADFGRF+VKTV+SRIYTLFSRSE
Sbjct: 121 AGVVSLGVPFLPPNPMRYRDLPEGFYIFRWKESGRAEADFGRFNVKTVLSRIYTLFSRSE 180

Query: 181 IPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMSN 240
           IPIAEKD+EIMDMVDESTPRPPWLTDEDL  Y  LYEHSGF S LQVPYRRKHKELG+SN
Sbjct: 181 IPIAEKDKEIMDMVDESTPRPPWLTDEDLTVYTTLYEHSGFESALQVPYRRKHKELGISN 240

Query: 241 PRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQVN 300
           PRVEVPVLLIIGGKDYFLKFPG+EDY+KS+KLREF+ADLEVADLADGTHFMQEQFPAQVN
Sbjct: 241 PRVEVPVLLIIGGKDYFLKFPGVEDYIKSEKLREFMADLEVADLADGTHFMQEQFPAQVN 300

Query: 301 QFLISFLSKHN 312
             LI+FL+KHN
Sbjct: 301 HLLINFLTKHN 310

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BLAST of Spg036456 vs. NCBI nr
Match: XP_022140163.1 (uncharacterized protein LOC111010895, partial [Momordica charantia])

HSP 1 Score: 505.8 bits (1301), Expect = 2.7e-139
Identity = 237/289 (82.01%), Postives = 265/289 (91.70%), Query Frame = 0


Query: 23  TGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGLSDPHPRPENATFEDFA 82
           TGS   +VF+HGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYG SDPHP+P+NA+F+DF 
Sbjct: 1   TGS-TVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFV 60

Query: 83  EDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAGIVSLGVPYLPPNPTRYRDLP 142
           +D L ILDFL I KAFLVGKDFGSWV YLFSL+FPARVAGIVSLGVP+  P+P RY++LP
Sbjct: 61  QDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLP 120

Query: 143 EGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSEIPIAEKDQEIMDMVDESTPRPP 202
           E FYI+RWKE+GRAEADFGRFDVKTV+ RIYTLF+RSEIPIAEKDQEIMDMVDESTP PP
Sbjct: 121 EDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPP 180

Query: 203 WLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMSNPRVEVPVLLIIGGKDYFLKFPG 262
           WL +EDLAAYA  Y++SGF STLQVPYRRKHKELG++NP+VEVPVLLIIG KDYFLKFPG
Sbjct: 181 WLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPG 240

Query: 263 IEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQVNQFLISFLSKHN 312
           IEDY+KS+K+REFVADLEVADLADGTHFMQEQFPAQ+N  LI+FLS H+
Sbjct: 241 IEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHH 288

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BLAST of Spg036456 vs. NCBI nr
Match: XP_015890165.1 (bifunctional epoxide hydrolase 2-like [Ziziphus jujuba])

HSP 1 Score: 476.5 bits (1225), Expect = 1.8e-130
Identity = 227/312 (72.76%), Postives = 258/312 (82.69%), Query Frame = 0


Query: 1   MDQIQHKFIKIRES--KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAIS 60
           MDQIQHK+I       KLH AEIGTGS   +VFIHGFPEIWYSWRHQM+A A AGYRAIS
Sbjct: 1   MDQIQHKYIYSDSGGVKLHIAEIGTGS-KVVVFIHGFPEIWYSWRHQMIAVAKAGYRAIS 60

Query: 61  PDLRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPA 120
           PDLRGYGLS PHP  +NA+F DF +DIL ILD L I KAFLVGKDFGSW  YLFSL  P 
Sbjct: 61  PDLRGYGLSHPHPNLQNASFNDFVQDILAILDSLNIHKAFLVGKDFGSWPVYLFSLTHPE 120

Query: 121 RVAGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSR 180
           RV+GI+SLGVP+LPPNP RY+DLPEGFYI RWKE+GRAEADF RFDVKT+V  IY LFSR
Sbjct: 121 RVSGIISLGVPFLPPNPQRYKDLPEGFYISRWKEAGRAEADFSRFDVKTIVRNIYILFSR 180

Query: 181 SEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGM 240
           SEIPIA+KD+EIMDMVD STP PPW T+EDLA YA LYE+SGF S +QVPY+   KE  +
Sbjct: 181 SEIPIAKKDEEIMDMVDSSTPLPPWFTNEDLATYATLYENSGFDSPMQVPYKGLRKESVI 240

Query: 241 SNPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQ 300
           +N ++E PVLL++GGKDYFLKFPGIE+Y+KS K+RE+V DLEV  LADGTHFMQEQ P Q
Sbjct: 241 TNLKIEAPVLLVMGGKDYFLKFPGIEEYVKSGKMREYVGDLEVKFLADGTHFMQEQLPEQ 300

Query: 301 VNQFLISFLSKH 311
           VNQ ++SFLS+H
Sbjct: 301 VNQLIVSFLSQH 311

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BLAST of Spg036456 vs. NCBI nr
Match: PON96090.1 (Epoxide hydrolase-like [Trema orientale])

HSP 1 Score: 465.7 bits (1197), Expect = 3.2e-127
Identity = 212/311 (68.17%), Postives = 257/311 (82.64%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQIQHK+I  R  KLH AEIGTGS  A+VF+HGFPEIWYSWRHQM+A A AGYRAI+PD
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGS-TAVVFLHGFPEIWYSWRHQMIAVAQAGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYGLS+PHP P+ A+F DF ED L ILDF  I KAFLVGKDFGSW  YLF L +P RV
Sbjct: 61  LRGYGLSEPHPEPQKASFNDFVEDTLSILDFFNIEKAFLVGKDFGSWPVYLFCLTYPTRV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
           AG++SLGVP+ PP+P RY+++PEGFYIFRWKE GRAEADFGRFDVKTV+  IY LFSRSE
Sbjct: 121 AGVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDVKTVLRNIYILFSRSE 180

Query: 181 IPIAEKDQEIMDMVD-ESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMS 240
           IPIAEKD+E MD+VD ++TP PPW T+EDL+ YA LY+ SGF S +QVPY+   +E  ++
Sbjct: 181 IPIAEKDKETMDLVDSDNTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFRIA 240

Query: 241 NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQV 300
            P++EVP ++I+GGKDYFLKFPGIE+Y K+ K+R++  + E+  LA+GTHFMQEQFPAQV
Sbjct: 241 KPKIEVPAMVIMGGKDYFLKFPGIEEYTKNGKMRDYAPNSEMKFLAEGTHFMQEQFPAQV 300

Query: 301 NQFLISFLSKH 311
           NQ +I+FL+ H
Sbjct: 301 NQLIITFLNDH 310

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BLAST of Spg036456 vs. NCBI nr
Match: PON36798.1 (Epoxide hydrolase-like [Parasponia andersonii])

HSP 1 Score: 463.4 bits (1191), Expect = 1.6e-126
Identity = 211/311 (67.85%), Postives = 255/311 (81.99%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQIQHK+I  R  KLH AEIGTGS  A+VF+HGFPEIWYSWRHQM+A A AGYRAI+PD
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGS-TAVVFLHGFPEIWYSWRHQMIAIAQAGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYGLS+PHP P+ A+F DF ED L ILDF +I KAFLVGKDFGSW  YLF L +P RV
Sbjct: 61  LRGYGLSEPHPEPQKASFNDFVEDTLSILDFFKIEKAFLVGKDFGSWPVYLFCLTYPTRV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
           AG++SLGVP+ PP+P RY+++PEGFYIFRWKE GRAEADFGRFD++TV+  IY LFSRSE
Sbjct: 121 AGVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDMRTVLRNIYILFSRSE 180

Query: 181 IPIAEKDQEIMDMVD-ESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMS 240
           IPIAE D+EIMD+VD   TP PPW T+EDL+ YA LY+ SGF S +QVPY+   +E G++
Sbjct: 181 IPIAETDKEIMDLVDSHDTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFGIA 240

Query: 241 NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQV 300
            P++EVP +LI+GGKDYFLKFPGIE+Y K+  +R++V + E   LA+GTHFMQEQFP QV
Sbjct: 241 KPKIEVPAMLIMGGKDYFLKFPGIEEYTKNGNMRDYVPNSETKFLAEGTHFMQEQFPEQV 300

Query: 301 NQFLISFLSKH 311
           NQ +I+FL+ H
Sbjct: 301 NQLIITFLNDH 310

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BLAST of Spg036456 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-32
Identity = 96/329 (29.18%), Postives = 162/329 (49.24%), Query Frame = 0


Query: 4   IQHKFIKIR-ESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLR 63
           + H ++ ++   +LHF E+G+G   A+   HGFPE WYSWR+Q+ A A AGYR ++ D++
Sbjct: 237 MSHGYVTVKPRVRLHFVELGSG--PAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMK 296

Query: 64  GYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAG 123
           GYG S   P  E    E   ++++  LD L +S+A  +G D+G  + +  +L +P RV  
Sbjct: 297 GYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRA 356

Query: 124 IVSLGVPYLPPNPT-----RYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFS 183
           + SL  P++P NP        +  P   Y   ++E G AEA     +++  +SR +    
Sbjct: 357 VASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEA-----ELEQNLSRTFKSLF 416

Query: 184 RSEIPIAEKDQEIMDM--------VDESTPRPPWL----TDEDLAAYAALYEHSGFGSTL 243
           R+       D+ ++ M        +  ++P  P L    T+E++  Y   ++ SGF   L
Sbjct: 417 RA------SDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPL 476

Query: 244 QVPYRRKH-------KELGMSNPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVAD 303
              YR          K LG    ++ +P L++   KD+ L  P +     S  + +++  
Sbjct: 477 NW-YRNMERNWKWACKSLGR---KILIPALMVTAEKDFVL-VPQM-----SQHMEDWIPH 536

Query: 304 LEVADLADGTHFMQEQFPAQVNQFLISFL 308
           L+   + D  H+ Q   P +VNQ LI +L
Sbjct: 537 LKRGHIEDCGHWTQMDKPTEVNQILIKWL 542

[Close]

BLAST of Spg036456 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.2e-32
Identity = 95/320 (29.69%), Postives = 157/320 (49.06%), Query Frame = 0


Query: 2   DQIQHKFIKIRES-KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 61
           + + H ++ ++   +LHF E+G+G   AI   HGFPE W+SWR+Q+ A A AG+R ++ D
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSG--PAICLCHGFPESWFSWRYQIPALAQAGFRVLAID 292

Query: 62  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 121
           ++GYG S   P  E    E   E+++  L+ L I +A  +G D+   + +  +L  P RV
Sbjct: 293 MKGYGDSSSPPEIEEYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERV 352

Query: 122 AGIVSLGVPYLPPNP-----TRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTL 181
             + SL  P +PPNP        R +P   Y   ++E G AEA+  +   +T  S   T 
Sbjct: 353 RAVASLNTPLMPPNPEVSPMEVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKSFFRTS 412

Query: 182 FSRSEIPIAEKDQEIMDMVDESTPRPPWL----TDEDLAAYAALYEHSGFGSTLQVPYRR 241
                + + +  +  M  +   TP  P +    T+E++  Y   ++ SGF   L   YR 
Sbjct: 413 DDMGLLTVNKATE--MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNW-YRN 472

Query: 242 KHKELGMS----NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADG 301
             +    S      ++ VP L++   KD  L+ P +     S  +  ++  L+   + D 
Sbjct: 473 TERNWKWSCKALGRKILVPALMVTAEKDIVLR-PEM-----SKNMENWIPFLKRGHIEDC 532

Query: 302 THFMQEQFPAQVNQFLISFL 308
            H+ Q + PA+VNQ LI +L
Sbjct: 533 GHWTQIEKPAEVNQILIKWL 541

[Close]

BLAST of Spg036456 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 138.7 bits (348), Expect = 1.2e-31
Identity = 93/320 (29.06%), Postives = 160/320 (50.00%), Query Frame = 0


Query: 2   DQIQHKFIKIRES-KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 61
           + + H ++ ++   +LHF E+G+G   A+   HGFPE W+SWR+Q+ A A AG+R ++ D
Sbjct: 233 NDVSHGYVTVKPGIRLHFVEMGSG--PALCLCHGFPESWFSWRYQIPALAQAGFRVLAID 292

Query: 62  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 121
           ++GYG S   P  E    E   ++++  LD L I +A  +G D+   + +  +L +P RV
Sbjct: 293 MKGYGDSSSPPEIEEYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERV 352

Query: 122 AGIVSLGVPYLPPNP-----TRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTL 181
             + SL  P++PP+P        R +P   Y   ++E G AEA+  +   +T  S  +  
Sbjct: 353 RAVASLNTPFMPPDPDVSPMKVIRSIPVFNYQLYFQEPGVAEAELEKNMSRTFKS-FFRA 412

Query: 182 FSRSEIPIAEKDQEIMDMVDESTPRPPWL----TDEDLAAYAALYEHSGFGSTLQVPYRR 241
              +      K  EI  ++  +TP  P L    T+E++  Y   ++ +GF   L   YR 
Sbjct: 413 SDETGFIAVHKATEIGGIL-VNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNW-YRN 472

Query: 242 KHKELGMS----NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADG 301
             +    S      ++ VP L++   KD  L+ P +     S  + +++  L+   + D 
Sbjct: 473 TERNWKWSCKGLGRKILVPALMVTAEKDIVLR-PEM-----SKNMEKWIPFLKRGHIEDC 532

Query: 302 THFMQEQFPAQVNQFLISFL 308
            H+ Q + P +VNQ LI +L
Sbjct: 533 GHWTQIEKPTEVNQILIKWL 541

[Close]

BLAST of Spg036456 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.7e-30
Identity = 90/320 (28.12%), Postives = 159/320 (49.69%), Query Frame = 0


Query: 4   IQHKFIKIRES-KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLR 63
           + H ++ I+   +LHF E+G+G   A+   HGFPE W+SWR+Q+ A A AG+R ++ D++
Sbjct: 237 LSHGYVLIKPGVRLHFVEMGSG--PAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMK 296

Query: 64  GYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAG 123
           GYG S   P  E  + E   +D++  L+ L +S+A  +G D+G  + +  +L +P RV  
Sbjct: 297 GYGESSAPPEIEEYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRA 356

Query: 124 IVSLGVPYLPPNPT-----RYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFS 183
           + SL  P++P NP        +  P   Y   ++E G AEA     +++  + R +  F 
Sbjct: 357 VASLNTPFMPSNPNVSPMEIIKANPVFDYQLYFQEPGVAEA-----ELEQNLDRTFKNFF 416

Query: 184 RSEIPIAEKDQEIMDM--VDESTPRPPWL----TDEDLAAYAALYEHSGFGSTLQVPYRR 243
           R+          + ++  +   TP  P L    T+ED+  Y   ++ SGF   L   YR 
Sbjct: 417 RAHDETFLTTNRVRELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNW-YRN 476

Query: 244 KHKELGM----SNPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADG 303
             +        S  ++ +P L++    D  L  P +  ++++     ++  L+   + D 
Sbjct: 477 MERNWQWGCKGSGRKILIPALMVTAENDLVL-HPKMSKHMEN-----WIPHLKRGHIKDC 536

Query: 304 THFMQEQFPAQVNQFLISFL 308
            H+ Q   PA++N+ LI +L
Sbjct: 537 GHWTQIDKPAELNRILIEWL 542

[Close]

BLAST of Spg036456 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.4e-29
Identity = 96/310 (30.97%), Postives = 144/310 (46.45%), Query Frame = 0


Query: 15  KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGLSDPHPRPE 74
           +L   E G   A  ++  HGFPE+ YSWRHQ+ A A AGY  ++PD RGYG S      E
Sbjct: 15  RLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSSRPEAIE 74

Query: 75  NATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAGIVSLGVPYLP-- 134
                    D++G+LD +   +A  VG D+G+ V +   LL   RVA + +L VP LP  
Sbjct: 75  AYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSVPALPRA 134

Query: 135 -PNPT---RYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTL-----FSRSEIPI 194
              PT   R R     FYI  ++E G A+A+      +T+   I  L      S +   +
Sbjct: 135 QVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQSAAMRML 194

Query: 195 AEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKE----LGMS 254
           A      +D + E    P W++ E+L  Y   +  +GF   L   YR   +       ++
Sbjct: 195 APGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNW-YRNFDRNWETTADLA 254

Query: 255 NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGT-HFMQEQFPAQ 309
              + VP L I G  D  L F       ++D+  E ++      L DG  H++Q++ P +
Sbjct: 255 GKTISVPSLFIAGTADPVLTF------TRTDRAAEVISGPYREVLIDGAGHWLQQERPGE 314

[Close]

BLAST of Spg036456 vs. ExPASy TrEMBL
Match: A0A6J1CHD9 (uncharacterized protein LOC111010895 OS=Momordica charantia OX=3673 GN=LOC111010895 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.3e-139
Identity = 237/289 (82.01%), Postives = 265/289 (91.70%), Query Frame = 0


Query: 23  TGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGLSDPHPRPENATFEDFA 82
           TGS   +VF+HGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYG SDPHP+P+NA+F+DF 
Sbjct: 1   TGS-TVVVFVHGFPEIWYSWRHQMVAAAAAGYRAISPDLRGYGFSDPHPQPQNASFDDFV 60

Query: 83  EDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAGIVSLGVPYLPPNPTRYRDLP 142
           +D L ILDFL I KAFLVGKDFGSWV YLFSL+FPARVAGIVSLGVP+  P+P RY++LP
Sbjct: 61  QDTLAILDFLHIPKAFLVGKDFGSWVVYLFSLIFPARVAGIVSLGVPFFAPDPKRYQNLP 120

Query: 143 EGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSEIPIAEKDQEIMDMVDESTPRPP 202
           E FYI+RWKE+GRAEADFGRFDVKTV+ RIYTLF+RSEIPIAEKDQEIMDMVDESTP PP
Sbjct: 121 EDFYIYRWKETGRAEADFGRFDVKTVLRRIYTLFARSEIPIAEKDQEIMDMVDESTPLPP 180

Query: 203 WLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMSNPRVEVPVLLIIGGKDYFLKFPG 262
           WL +EDLAAYA  Y++SGF STLQVPYRRKHKELG++NP+VEVPVLLIIG KDYFLKFPG
Sbjct: 181 WLNEEDLAAYATAYQNSGFQSTLQVPYRRKHKELGITNPKVEVPVLLIIGRKDYFLKFPG 240

Query: 263 IEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQVNQFLISFLSKHN 312
           IEDY+KS+K+REFVADLEVADLADGTHFMQEQFPAQ+N  LI+FLS H+
Sbjct: 241 IEDYIKSEKMREFVADLEVADLADGTHFMQEQFPAQLNHLLIAFLSNHH 288

[Close]

BLAST of Spg036456 vs. ExPASy TrEMBL
Match: A0A6P4AMV9 (bifunctional epoxide hydrolase 2-like OS=Ziziphus jujuba OX=326968 GN=LOC107424812 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 8.6e-131
Identity = 227/312 (72.76%), Postives = 258/312 (82.69%), Query Frame = 0


Query: 1   MDQIQHKFIKIRES--KLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAIS 60
           MDQIQHK+I       KLH AEIGTGS   +VFIHGFPEIWYSWRHQM+A A AGYRAIS
Sbjct: 1   MDQIQHKYIYSDSGGVKLHIAEIGTGS-KVVVFIHGFPEIWYSWRHQMIAVAKAGYRAIS 60

Query: 61  PDLRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPA 120
           PDLRGYGLS PHP  +NA+F DF +DIL ILD L I KAFLVGKDFGSW  YLFSL  P 
Sbjct: 61  PDLRGYGLSHPHPNLQNASFNDFVQDILAILDSLNIHKAFLVGKDFGSWPVYLFSLTHPE 120

Query: 121 RVAGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSR 180
           RV+GI+SLGVP+LPPNP RY+DLPEGFYI RWKE+GRAEADF RFDVKT+V  IY LFSR
Sbjct: 121 RVSGIISLGVPFLPPNPQRYKDLPEGFYISRWKEAGRAEADFSRFDVKTIVRNIYILFSR 180

Query: 181 SEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGM 240
           SEIPIA+KD+EIMDMVD STP PPW T+EDLA YA LYE+SGF S +QVPY+   KE  +
Sbjct: 181 SEIPIAKKDEEIMDMVDSSTPLPPWFTNEDLATYATLYENSGFDSPMQVPYKGLRKESVI 240

Query: 241 SNPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQ 300
           +N ++E PVLL++GGKDYFLKFPGIE+Y+KS K+RE+V DLEV  LADGTHFMQEQ P Q
Sbjct: 241 TNLKIEAPVLLVMGGKDYFLKFPGIEEYVKSGKMREYVGDLEVKFLADGTHFMQEQLPEQ 300

Query: 301 VNQFLISFLSKH 311
           VNQ ++SFLS+H
Sbjct: 301 VNQLIVSFLSQH 311

[Close]

BLAST of Spg036456 vs. ExPASy TrEMBL
Match: A0A2P5FE57 (Epoxide hydrolase-like OS=Trema orientale OX=63057 GN=TorRG33x02_080200 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.5e-127
Identity = 212/311 (68.17%), Postives = 257/311 (82.64%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQIQHK+I  R  KLH AEIGTGS  A+VF+HGFPEIWYSWRHQM+A A AGYRAI+PD
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGS-TAVVFLHGFPEIWYSWRHQMIAVAQAGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYGLS+PHP P+ A+F DF ED L ILDF  I KAFLVGKDFGSW  YLF L +P RV
Sbjct: 61  LRGYGLSEPHPEPQKASFNDFVEDTLSILDFFNIEKAFLVGKDFGSWPVYLFCLTYPTRV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
           AG++SLGVP+ PP+P RY+++PEGFYIFRWKE GRAEADFGRFDVKTV+  IY LFSRSE
Sbjct: 121 AGVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDVKTVLRNIYILFSRSE 180

Query: 181 IPIAEKDQEIMDMVD-ESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMS 240
           IPIAEKD+E MD+VD ++TP PPW T+EDL+ YA LY+ SGF S +QVPY+   +E  ++
Sbjct: 181 IPIAEKDKETMDLVDSDNTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFRIA 240

Query: 241 NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQV 300
            P++EVP ++I+GGKDYFLKFPGIE+Y K+ K+R++  + E+  LA+GTHFMQEQFPAQV
Sbjct: 241 KPKIEVPAMVIMGGKDYFLKFPGIEEYTKNGKMRDYAPNSEMKFLAEGTHFMQEQFPAQV 300

Query: 301 NQFLISFLSKH 311
           NQ +I+FL+ H
Sbjct: 301 NQLIITFLNDH 310

[Close]

BLAST of Spg036456 vs. ExPASy TrEMBL
Match: A0A2P5AJS1 (Epoxide hydrolase-like OS=Parasponia andersonii OX=3476 GN=PanWU01x14_325590 PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 7.6e-127
Identity = 211/311 (67.85%), Postives = 255/311 (81.99%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQIQHK+I  R  KLH AEIGTGS  A+VF+HGFPEIWYSWRHQM+A A AGYRAI+PD
Sbjct: 1   MDQIQHKYIDARGVKLHIAEIGTGS-TAVVFLHGFPEIWYSWRHQMIAIAQAGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYGLS+PHP P+ A+F DF ED L ILDF +I KAFLVGKDFGSW  YLF L +P RV
Sbjct: 61  LRGYGLSEPHPEPQKASFNDFVEDTLSILDFFKIEKAFLVGKDFGSWPVYLFCLTYPTRV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
           AG++SLGVP+ PP+P RY+++PEGFYIFRWKE GRAEADFGRFD++TV+  IY LFSRSE
Sbjct: 121 AGVISLGVPFFPPDPQRYKNMPEGFYIFRWKEPGRAEADFGRFDMRTVLRNIYILFSRSE 180

Query: 181 IPIAEKDQEIMDMVD-ESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMS 240
           IPIAE D+EIMD+VD   TP PPW T+EDL+ YA LY+ SGF S +QVPY+   +E G++
Sbjct: 181 IPIAETDKEIMDLVDSHDTPLPPWFTEEDLSFYATLYQKSGFDSPMQVPYKNPREEFGIA 240

Query: 241 NPRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQV 300
            P++EVP +LI+GGKDYFLKFPGIE+Y K+  +R++V + E   LA+GTHFMQEQFP QV
Sbjct: 241 KPKIEVPAMLIMGGKDYFLKFPGIEEYTKNGNMRDYVPNSETKFLAEGTHFMQEQFPEQV 300

Query: 301 NQFLISFLSKH 311
           NQ +I+FL+ H
Sbjct: 301 NQLIITFLNDH 310

[Close]

BLAST of Spg036456 vs. ExPASy TrEMBL
Match: A0A061GFG9 (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_029912 PE=4 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.0e-123
Identity = 211/309 (68.28%), Postives = 245/309 (79.29%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           MDQIQHK I +R   LH AEIGTGS + +VF+HGFPEIWYSWRHQM+A A AGYRAI+PD
Sbjct: 1   MDQIQHKHIDVRGVNLHVAEIGTGS-SVVVFLHGFPEIWYSWRHQMIAIAGAGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYGLS  HP PE A+F DF +D + ILD   I KAFL+GKDFGSW  YL SL  P RV
Sbjct: 61  LRGYGLSGNHPEPEKASFNDFVDDTIAILDSFHIDKAFLIGKDFGSWPVYLLSLFHPTRV 120

Query: 121 AGIVSLGVPYLPPNPTRYRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSRSE 180
            G+VSLGVP+  P P ++RDLPEGFYI RWKE GRAEADF RFDVKTV   IY LFSRSE
Sbjct: 121 IGVVSLGVPFFVPRPQKFRDLPEGFYISRWKEPGRAEADFSRFDVKTVWRNIYILFSRSE 180

Query: 181 IPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGMSN 240
           IPIAEK +EIMD+VD S P PPWLT+EDL  YA  YE SGF S +QVPY R  +E  + N
Sbjct: 181 IPIAEKGKEIMDLVDPSNPFPPWLTNEDLTTYAKAYEKSGFSSPMQVPYTRLPEEFTIEN 240

Query: 241 PRVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGTHFMQEQFPAQVN 300
           P+VEVPVLLI+GGKDYFLKFPGIEDY+ S+K+R++VA+LE+  L +GTHFMQEQFP +VN
Sbjct: 241 PKVEVPVLLIVGGKDYFLKFPGIEDYITSEKVRDYVAELEIEFLPEGTHFMQEQFPDKVN 300

Query: 301 QFLISFLSK 310
           Q +++FL K
Sbjct: 301 QLIVNFLDK 308

[Close]

BLAST of Spg036456 vs. TAIR 10
Match: AT3G51000.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-52
Identity = 109/316 (34.49%), Postives = 177/316 (56.01%), Query Frame = 0


Query: 4   IQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 63
           ++ K IK     L+ AE G      ++ +HGFPE WYSWRHQ+   ++ GY  ++PDLRG
Sbjct: 5   VREKKIKTNGIWLNVAEKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRG 64

Query: 64  YGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARVAGI 123
           YG SD  P  E+ T      D++G+LD    ++AF+ G D+G+ + +   L  P RV G 
Sbjct: 65  YGDSDSLPSHESYTVSHLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGF 124

Query: 124 VSLGVPYLPPNPTR-----YRDLPEGFYIFRWKESGRAEADFGRFDVKTVVSRIYTLFSR 183
           +SL VPY P +P       ++   +G YI ++++ GRAEA F + D  +V+ + + L +R
Sbjct: 125 ISLSVPYFPRDPKLKPSDFFKIFGDGLYITQFQKPGRAEAAFAKHDCLSVMKK-FLLITR 184

Query: 184 SEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHKELGM 243
           ++  +A  D EI+D ++  +  P W+T+E++  YA  ++ SGF   L   YR       +
Sbjct: 185 TDYLVAPPDTEIIDHLEIPSTIPDWITEEEIQVYAEKFQRSGFTGPLNY-YRSMDMNWEI 244

Query: 244 SNP----RVEVPVLLIIGGKDYFLKFP-GIEDYLKSDKLREFVADLEVADLADGTHFMQE 303
             P    ++ VP   I G KD   + P G  +Y+K +  +  V +LE+  +  G HF+Q+
Sbjct: 245 LAPWQDSKIVVPTKFIAGDKDIGYEGPNGTMEYVKGEVFKIVVPNLEIVVIEGGHHFIQQ 304

Query: 304 QFPAQVNQFLISFLSK 310
           +   QV+Q ++SFL+K
Sbjct: 305 EKSEQVSQEILSFLNK 318

[Close]

BLAST of Spg036456 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.6 bits (501), Expect = 1.5e-50
Identity = 111/320 (34.69%), Postives = 175/320 (54.69%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPD 60
           M++I+H  I      +H A IG+G    I+F+HGFP++WYSWRHQ+V+ AA GYRAI+PD
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSG--PVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPD 60

Query: 61  LRGYGLSDPHPRPENATFEDFAEDILGILDFLQISKAFLVGKDFGSWVAYLFSLLFPARV 120
           LRGYG SD  P  E+ T      D++G+LD L + + FLVG D+G+ VA+   ++ P RV
Sbjct: 61  LRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRV 120

Query: 121 AGIVSLGVPYLPPNPT-----RYRDL-PEGFYIFRWKESGRAEADFGRFDVKTVVSRIYT 180
             +V+  V + P NP+      +R L  + +YI R++E G  E DF + D K +++R +T
Sbjct: 121 NALVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFT 180

Query: 181 LFSRSEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKHK 240
             SR+  P           + +    P WLT++D+  Y   +   GF   L   YR  + 
Sbjct: 181 --SRNPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNY-YRALNL 240

Query: 241 ELGMSNP----RVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADGT-H 300
              ++ P    +++VPV  I+G  D     PG ++Y+    L++ V  L+   + +G  H
Sbjct: 241 SWELTAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGH 300

Query: 301 FMQEQFPAQVNQFLISFLSK 310
           F+ ++ P +V   +  F  K
Sbjct: 301 FLHQEKPDEVTDHIYGFFKK 315

[Close]

BLAST of Spg036456 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 182.6 bits (462), Expect = 5.0e-46
Identity = 102/322 (31.68%), Postives = 172/322 (53.42%), Query Frame = 0


Query: 4   IQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 63
           ++H+ ++     +H A  G      ++ +HGFPE+WYSWRHQ+   AA GYRA++PDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGLSDPHPRPENATFEDFAEDILGILDFLQIS---KAFLVGKDFGSWVAYLFSLLFPARV 123
           YG SD      + T  +   D++ ++  L  S   K F+VG D+G+ +A+   L  P RV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 AGIVSLGVP--YLPPNPT-----RYRDL-PEGFYIFRWKESGRAEADFGRFDVKTVVSRI 183
             +V+L VP  + P +P+     R R    + +YI R++E G  EA+      + V+ R+
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 YTLFSRSEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRK 243
            T  +   + I  KD+       E+ P P WLT+ED+A + + +E  GF   +   YR  
Sbjct: 181 LTYRTPGPV-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNY-YRNF 240

Query: 244 HKELGMSNP----RVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADG- 303
           ++   +  P    +++VP   +IG  D     PG+++Y+   + +E V  LE   + +G 
Sbjct: 241 NRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGV 300

Query: 304 THFMQEQFPAQVNQFLISFLSK 310
            HF+ ++ P ++ Q ++ F+SK
Sbjct: 301 AHFINQEKPQEILQIILDFISK 320

[Close]

BLAST of Spg036456 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 9.4e-45
Identity = 99/325 (30.46%), Postives = 171/325 (52.62%), Query Frame = 0


Query: 1   MDQIQHKFIKIRESKLHFAE---IGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAI 60
           +D ++HK +K+    +H AE    G+G    I+F+HGFPE+WY+WRHQMVA ++ GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  SPDLRGYGLSDPHPRPENATFEDFAEDILGILDFLQ--ISKAFLVGKDFGSWVAYLFSLL 120
           +PDLRGYG ++   + E+ T+ +   D++ ++D +        +VG D+G+ +A+     
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 FPARVAGIVSLGVPYLPPNPTRY------RDLPEGFYIFRWKESGRAEADFGRFDVKTVV 180
            P +V  +V++ V + P NP R           + +Y+ R++++G  E +F +   + V+
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 SRIYTLFSRSEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPY 240
               T  +   + +  KD+      + ++  P WLT EDL  Y   YE+ GF   +   Y
Sbjct: 231 KEFLTYKTPGPLNL-PKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINY-Y 290

Query: 241 RRKHKELGMSNP----RVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLA 300
           R   +   ++ P    ++ VPV  IIG +D    FPG ++Y+     +  V  L+   + 
Sbjct: 291 RNIDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVL 350

Query: 301 DGT-HFMQEQFPAQVNQFLISFLSK 310
            G  HF+ E+ P  +NQ + +F  K
Sbjct: 351 KGLGHFLHEENPDVINQHIHNFFHK 373

[Close]

BLAST of Spg036456 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.8e-43
Identity = 96/320 (30.00%), Postives = 171/320 (53.44%), Query Frame = 0


Query: 4   IQHKFIKIRESKLHFAEIGTGSAAAIVFIHGFPEIWYSWRHQMVAAAAAGYRAISPDLRG 63
           ++H+ ++     +H A  G      ++ +HGFPE+WYSWRHQ+   AA GYRA++PDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGLSDPHPRPENATFEDFAEDILGILDFL--QISKAFLVGKDFGSWVAYLFSLLFPARVA 123
           YG SD      + T  +   D++ ++  L  +  K F+VG D+G+ +A+   L  P +V 
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 GIVSLGVP--YLPPNPT-----RYRDL-PEGFYIFRWKESGRAEADFGRFDVKTVVSRIY 183
            +V+L VP  + P +P+     R R +    +Y+ R++E G  EA+      + V+ R+ 
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TLFSRSEIPIAEKDQEIMDMVDESTPRPPWLTDEDLAAYAALYEHSGFGSTLQVPYRRKH 243
           T  +   + I  KD+       E+ P P WLT+ED+A + + ++  GF   +   YR  +
Sbjct: 181 TYRTPGPL-IIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNY-YRNFN 240

Query: 244 KELGMSNP----RVEVPVLLIIGGKDYFLKFPGIEDYLKSDKLREFVADLEVADLADG-T 303
           +   +  P    +++VP   +IG  D     PG+++Y+   + +E V  +E   + +G  
Sbjct: 241 RNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVA 300

Query: 304 HFMQEQFPAQVNQFLISFLS 309
           HF+ ++ P ++ Q ++ F+S
Sbjct: 301 HFLNQEKPQEILQIILDFIS 318

The following BLAST results are available for this feature:

BLAST of Spg036456 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905360.1	1.7e-157	85.53	bifunctional epoxide hydrolase 2-like [Benincasa hispida]	[more]
XP_022140163.1	2.7e-139	82.01	uncharacterized protein LOC111010895, partial [Momordica charantia]	[more]
XP_015890165.1	1.8e-130	72.76	bifunctional epoxide hydrolase 2-like [Ziziphus jujuba]	[more]
PON96090.1	3.2e-127	68.17	Epoxide hydrolase-like [Trema orientale]	[more]
PON36798.1	1.6e-126	67.85	Epoxide hydrolase-like [Parasponia andersonii]	[more]

BLAST of Spg036456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34913	1.8e-32	29.18	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
P80299	5.2e-32	29.69	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=...	[more]
P34914	1.2e-31	29.06	Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2	[more]
Q6Q2C2	1.7e-30	28.13	Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1	[more]
I6YGS0	2.4e-29	30.97	Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX...	[more]

BLAST of Spg036456 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHD9	1.3e-139	82.01	uncharacterized protein LOC111010895 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6P4AMV9	8.6e-131	72.76	bifunctional epoxide hydrolase 2-like OS=Ziziphus jujuba OX=326968 GN=LOC1074248...	[more]
A0A2P5FE57	1.5e-127	68.17	Epoxide hydrolase-like OS=Trema orientale OX=63057 GN=TorRG33x02_080200 PE=4 SV=...	[more]
A0A2P5AJS1	7.6e-127	67.85	Epoxide hydrolase-like OS=Parasponia andersonii OX=3476 GN=PanWU01x14_325590 PE=...	[more]
A0A061GFG9	3.0e-123	68.28	Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao OX=3641 GN=TCM_0299...	[more]

BLAST of Spg036456 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51000.1	3.5e-52	34.49	alpha/beta-Hydrolases superfamily protein	[more]
AT4G02340.1	1.5e-50	34.69	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	5.0e-46	31.68	soluble epoxide hydrolase	[more]
AT4G15960.1	9.4e-45	30.46	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26750.1	1.8e-43	30.00	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 33..51 score: 52.87 coord: 99..112 score: 28.9 coord: 113..126 score: 32.79 coord: 284..306 score: 26.28 coord: 245..261 score: 28.61 coord: 53..68 score: 55.97
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 99..112 score: 33.74 coord: 53..68 score: 43.53 coord: 113..126 score: 23.89
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 28..255 e-value: 2.4E-20 score: 73.2
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 1..311 e-value: 1.0E-79 score: 270.6
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 2..309
None	No IPR available	PANTHER	PTHR43329:SF93	BIFUNCTIONAL EPOXIDE HYDROLASE 2-LIKE	coord: 2..310
None	No IPR available	PANTHER	PTHR43329	EPOXIDE HYDROLASE	coord: 2..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036456.1	Spg036456.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004715	non-membrane spanning protein tyrosine kinase activity
molecular_function	GO:0003824	catalytic activity