Spg035950 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg035950
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationscaffold5: 40430954 .. 40433976 (+)
RNA-Seq ExpressionSpg035950
SyntenySpg035950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGATGCAACCTCTTTCCTCACCGTCCGATCCCCGTCTAGATCTCCAAATCTACGATCCAACGGTGAACAGCCATCGATCAAAACTGATCTCGTAGAAGGCGCCGCAGTGGATTTTGCCCCTCTGTAGTGGCATTACGAGATTGTCATTTGTCTGTGAAGCCATGGCAGGGAGAAGCTCCATTGAGTGGGAGATCAGTGAGTGAAGAATCAGAGAAGAAACGAAGAATTTTTGTGTAATGGGTTTCACAATGGGCTTGAATTTGCTTCTTCTCGTTGCTATGGTGGCCACCAATATTCTTTCTCTTTACCATCTCTCCTCCACGCTTCAATCCACTAAATCCCCTGTTTCTCAGCCGGTTCCCGATCACCTAATCCGGCAGCTTCAGACCATACGCGCCACCATCAATCACCTCACGCGCCTCCACCCGACGGCGGCGGCCTCCGCATCCAAAACCAAACGCTCCATCCCTTCAGATCTCGTTCTGTACTCCCAATTTTCCCCGATTGCCTCGTCTTGCCATGGTAATCCCGAGCTTCTTCATAGGTTTATGAATTACACTCCATTTTCGAGTTGCCCTTCTGATTCTGATCTCGCCGAGGCTTTGATTCTTCGTGGGTGTCATCCACTTCCTCGTCGGCGGTGCTTTGCCAAAACCCCACAAAAACCCTCTTCTTCTTTGCCCCAAAATCCCTTTGCTTCTTCGATTCCGGAGTCCAACATTATTTGGGGGAAATATTCGTGTAAGGGTTTTGGCTGTTTGAATCGATTGAATCCGAATCTGGGGTTCGACCCTTCTCATGAAATCACCAAATTTATGACCTACAAGACGGAATTGGACCTTCCAATCCCTCAATTGCTGCAGATTGCGAAGGCGGCTAACTCTGTTCTTCGTCTTGGCCTTGACATTGGCGGTGGAACTGCCACTTTTGCTGCTAGATTGAAGCTTTACAATGTCACTATGGTCACAACGACTATGAACCTTGGCGCGCCGTACAATGAGGTTGCGGCGCTTAGAGGGTTGGTTCCTTTGCACGTGCCGCTGCAGCAGCGGTTGCCTATTTTTGATGGAGTGATGGATCTGGTTCGGTGTGGCCATGCTGTGAACCGATGGATTCCTGTGAAATCGATGGAGTTTTTGTTCTATGATGTGGACAGAGTGTTGAGAGTTGGGGGCTATCTTTGGTTTGATCATTTCTTCAGTAAAGGGGTGGATCTTGATAAGGTTTACTCTCCTTTGATCACCAAGTTGGGGTACCGGAAGGTCAAGTGGGCGACGGCGAATAAGACCGATTCCGGCGGGTTGAAGAACGGAGAGGTTTACTTGACTGCATTGCTGCAAAAGCCAGTGCCAACATGAGGTAATGTTGATTTCTCTAGTAGGGATGTTGTTTGTTGTTCTGCATTTTGGTTTTGAAGTGGATTAGACTTGATTTGGTTTCATAGCCTGCAAAGGAAGTGATTCTGGTTGTGTTGTAAGCTATGTTAATGGATTTGGAAATGTGGCACTTGAGAGAAGTCGAAGAAATGAGATAGATCTTGATTTAACATCTTAGGAAAACTTAGGATATGATAGAAAAGTTGAATGATTTAGGCTAATCCGTTCGTTTCTAGGCATGATTATTTGTGTCATAACATGTTTTGGAGCATGATTTTGGTCAAAGATGAAGAAACTTGTATGAAGTTTTGGCTACAATTGTTTTCGGTTGACTAAAAACGCCATTTTAGGAGCAGGGTTTAAGCTGATCCTATCTTCTCAGTAGCATTCCTATGTTTTTGTGTTTGTTTTTTTGGTTGTGTTCTGGCTTGCATTTGAGTTTTTGAATAGATTGATGAATCTTGTTAAGTGAGGAAGATGCATAGGAAAACTGTTACTTGGCTATGTTTTCAAAATCATCGATATCACTCTAGATCGCCCCGATCAATCACCAAAGATCGAATTCATTAGCCAGCTACTGATCTCTTCCATTAGTCAAGATAAAAGTGCCCCTCTTTTTTCTATTATTCATAGTGCTTTGGCAAAACCATCTTGAAGACCTAGACTAAGGCATAGGCAGGCTCTGATTGGTAATGTTTCCATTGCTTCAAATTTCCCTTTTGAAGAACATGTGTGTCAATGATGATTCCTTTTACCTCATCTGAAGATTATAACTCATTATTTGGGATTTTTTCTTCTTGTTCTTGATTTTTCAAAACATTAAGGATATTATCATTTTTATTGTTTTTAGCTCATAAGATTTATATATAAAATTAAAAAAAAACTAAATTAATCACTGAACACATTTAAAAATAAAAAGTATGTAGTTATGAAATACCATTTAATTTCTTATTCTAAAGAAAGAGAAATGAAAATATTACCAAATAAGCTAATTGATTCTTGGATTGACTTTGTCTAATGAGTTGAAAACATTTTAATTGGAGAAGAAACTAGCTCAAGTGGTTTCTTCTCACTTATTTGTTGCTATGAGATAAAAACTTATTTGATTATTCCAAACTTCTGATCTCATAAATTGACATAAAACCAGAAATTTTCAAATCATTGTCACAGGCAGTAACAGAGAACCTGCGGCTTGGATTATACGAGGAAACAATTATTTAATGAAATAGTCAAATTCTTGTGTAAGAAGATGGGATTTTACATCAAAACTCAAATATTTATCCACCACCATGGCCTGCTCATCTGGCAAAGCTACTGAGTGTTTGAAGAAGAAGAAACTAATGGTGGTTGAAGAAAGGCATCCAGAACAGATTTGTGGAGATTTATGTTTCAAATGGAAGTTGTTACAGTTAGAAATGGTTGCTTTATTGTAGGTTCTGTCGGATTTATATATGGTTTTGTTTTATTGCTCTTGAATTGTTGGTTGTATTGTAGAAGTTAGGAACTGACTATTCAAAATTGTAATAGTCCAAGTTCAGGAGCAGAAGTGATTAGACCTTCTTAATTTAGCATTTTGTTTTCCCTTTTTTTGGTAAAATTTGTTATATTAAATTGATATTTCAGTAAATTATATTTGAATTTGGAAC

mRNA sequence

ATGGGTTTCACAATGGGCTTGAATTTGCTTCTTCTCGTTGCTATGGTGGCCACCAATATTCTTTCTCTTTACCATCTCTCCTCCACGCTTCAATCCACTAAATCCCCTGTTTCTCAGCCGGTTCCCGATCACCTAATCCGGCAGCTTCAGACCATACGCGCCACCATCAATCACCTCACGCGCCTCCACCCGACGGCGGCGGCCTCCGCATCCAAAACCAAACGCTCCATCCCTTCAGATCTCGTTCTGTACTCCCAATTTTCCCCGATTGCCTCGTCTTGCCATGGTAATCCCGAGCTTCTTCATAGGTTTATGAATTACACTCCATTTTCGAGTTGCCCTTCTGATTCTGATCTCGCCGAGGCTTTGATTCTTCGTGGGTGTCATCCACTTCCTCGTCGGCGGTGCTTTGCCAAAACCCCACAAAAACCCTCTTCTTCTTTGCCCCAAAATCCCTTTGCTTCTTCGATTCCGGAGTCCAACATTATTTGGGGGAAATATTCGTGTAAGGGTTTTGGCTGTTTGAATCGATTGAATCCGAATCTGGGGTTCGACCCTTCTCATGAAATCACCAAATTTATGACCTACAAGACGGAATTGGACCTTCCAATCCCTCAATTGCTGCAGATTGCGAAGGCGGCTAACTCTGTTCTTCGTCTTGGCCTTGACATTGGCGGTGGAACTGCCACTTTTGCTGCTAGATTGAAGCTTTACAATGTCACTATGGTCACAACGACTATGAACCTTGGCGCGCCGTACAATGAGGTTGCGGCGCTTAGAGGGTTGGTTCCTTTGCACGTGCCGCTGCAGCAGCGGTTGCCTATTTTTGATGGAGTGATGGATCTGGTTCGGTGTGGCCATGCTGTGAACCGATGGATTCCTGTGAAATCGATGGAGTTTTTGTTCTATGATGTGGACAGAGTGTTGAGAGTTGGGGGCTATCTTTGGTTTGATCATTTCTTCAGTAAAGGGGTGGATCTTGATAAGGTTTACTCTCCTTTGATCACCAAGTTGGGGTACCGGAAGGTCAAGTGGGCGACGGCGAATAAGACCGATTCCGGCGGGTTGAAGAACGGAGAGGTTTACTTGACTGCATTGCTGCAAAAGCCAGTGCCAACATGA

Coding sequence (CDS)

ATGGGTTTCACAATGGGCTTGAATTTGCTTCTTCTCGTTGCTATGGTGGCCACCAATATTCTTTCTCTTTACCATCTCTCCTCCACGCTTCAATCCACTAAATCCCCTGTTTCTCAGCCGGTTCCCGATCACCTAATCCGGCAGCTTCAGACCATACGCGCCACCATCAATCACCTCACGCGCCTCCACCCGACGGCGGCGGCCTCCGCATCCAAAACCAAACGCTCCATCCCTTCAGATCTCGTTCTGTACTCCCAATTTTCCCCGATTGCCTCGTCTTGCCATGGTAATCCCGAGCTTCTTCATAGGTTTATGAATTACACTCCATTTTCGAGTTGCCCTTCTGATTCTGATCTCGCCGAGGCTTTGATTCTTCGTGGGTGTCATCCACTTCCTCGTCGGCGGTGCTTTGCCAAAACCCCACAAAAACCCTCTTCTTCTTTGCCCCAAAATCCCTTTGCTTCTTCGATTCCGGAGTCCAACATTATTTGGGGGAAATATTCGTGTAAGGGTTTTGGCTGTTTGAATCGATTGAATCCGAATCTGGGGTTCGACCCTTCTCATGAAATCACCAAATTTATGACCTACAAGACGGAATTGGACCTTCCAATCCCTCAATTGCTGCAGATTGCGAAGGCGGCTAACTCTGTTCTTCGTCTTGGCCTTGACATTGGCGGTGGAACTGCCACTTTTGCTGCTAGATTGAAGCTTTACAATGTCACTATGGTCACAACGACTATGAACCTTGGCGCGCCGTACAATGAGGTTGCGGCGCTTAGAGGGTTGGTTCCTTTGCACGTGCCGCTGCAGCAGCGGTTGCCTATTTTTGATGGAGTGATGGATCTGGTTCGGTGTGGCCATGCTGTGAACCGATGGATTCCTGTGAAATCGATGGAGTTTTTGTTCTATGATGTGGACAGAGTGTTGAGAGTTGGGGGCTATCTTTGGTTTGATCATTTCTTCAGTAAAGGGGTGGATCTTGATAAGGTTTACTCTCCTTTGATCACCAAGTTGGGGTACCGGAAGGTCAAGTGGGCGACGGCGAATAAGACCGATTCCGGCGGGTTGAAGAACGGAGAGGTTTACTTGACTGCATTGCTGCAAAAGCCAGTGCCAACATGA

Protein sequence

MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLTRLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNPNLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGEVYLTALLQKPVPT
Homology
BLAST of Spg035950 vs. NCBI nr
Match: XP_008443998.1 (PREDICTED: uncharacterized protein LOC103487447 [Cucumis melo] >XP_008444006.1 PREDICTED: uncharacterized protein LOC103487447 [Cucumis melo] >KAA0057105.1 Methyltransf_29 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 740.7 bits (1911), Expect = 6.1e-210
Identity = 363/373 (97.32%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAASASKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPTAAASASKTKLSIPSDLVLYSQFSPIASSCHSNPELLHKFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNI+WGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNILWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYD+DRVLRVGGYLWFDHFFSKGVDLDK+YSPLITKLGYRKVKWATANKTDSGGLKNGE
Sbjct: 301 LFYDLDRVLRVGGYLWFDHFFSKGVDLDKLYSPLITKLGYRKVKWATANKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVP+
Sbjct: 361 VYLTALLQKPVPS 373

BLAST of Spg035950 vs. NCBI nr
Match: XP_038895531.1 (uncharacterized protein LOC120083747 [Benincasa hispida] >XP_038895532.1 uncharacterized protein LOC120083747 [Benincasa hispida])

HSP 1 Score: 740.3 bits (1910), Expect = 7.9e-210
Identity = 363/373 (97.32%), Postives = 370/373 (99.20%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAAS SKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSD+A
Sbjct: 61  RLHPTAAASTSKTKLSIPSDLVLYSQFSPIASSCHSNPELLHKFMNYTPFSSCPSDSDVA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNIIWGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNIIWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSV+RLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVVRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYDVDRVLRVGGYLWFDHFFSKG+DLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE
Sbjct: 301 LFYDVDRVLRVGGYLWFDHFFSKGLDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVPT
Sbjct: 361 VYLTALLQKPVPT 373

BLAST of Spg035950 vs. NCBI nr
Match: XP_004147405.1 (uncharacterized protein LOC101208739 [Cucumis sativus] >XP_004147406.1 uncharacterized protein LOC101208739 [Cucumis sativus] >KGN65567.1 hypothetical protein Csa_019837 [Cucumis sativus])

HSP 1 Score: 733.4 bits (1892), Expect = 9.7e-208
Identity = 361/373 (96.78%), Postives = 369/373 (98.93%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAASASKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPTAAASASKTKLSIPSDLVLYSQFSPIASSCHTNPELLHKFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNIIW KYSCKG GCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNIIWEKYSCKGLGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYD+DRVLRVGGYLWFDHFFSKGVDLDK+YSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDLDRVLRVGGYLWFDHFFSKGVDLDKLYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVP+
Sbjct: 361 VYLTALLQKPVPS 373

BLAST of Spg035950 vs. NCBI nr
Match: XP_023520344.1 (uncharacterized protein LOC111783659 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 730.7 bits (1885), Expect = 6.3e-207
Identity = 359/372 (96.51%), Postives = 366/372 (98.39%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQS KSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSQKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHP AAAS SKTK SIPSDLVLYSQFSPIASSCH NPELLHRFMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPKAAASISKTKVSIPSDLVLYSQFSPIASSCHSNPELLHRFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPF SS+PESNIIWGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFGSSLPESNIIWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFM++KTELDLPIPQLLQIAKAANSV+RLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMSFKTELDLPIPQLLQIAKAANSVIRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVP 373
           VYLTALLQKP+P
Sbjct: 361 VYLTALLQKPMP 372

BLAST of Spg035950 vs. NCBI nr
Match: XP_022927546.1 (uncharacterized protein LOC111434341 [Cucurbita moschata])

HSP 1 Score: 730.7 bits (1885), Expect = 6.3e-207
Identity = 359/372 (96.51%), Postives = 366/372 (98.39%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQS KSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSQKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHP AAAS SKTK SIPSDLVLYSQFSPIASSCH NPELLHRFMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPKAAASVSKTKVSIPSDLVLYSQFSPIASSCHSNPELLHRFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPF SS+PESNIIWGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFGSSLPESNIIWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFM++KTELDLPIPQLLQIAKAANSV+RLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMSFKTELDLPIPQLLQIAKAANSVVRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVP 373
           VYLTALLQKP+P
Sbjct: 361 VYLTALLQKPMP 372

BLAST of Spg035950 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 2.1e-136
Identity = 249/386 (64.51%), Postives = 292/386 (75.65%), Query Frame = 0

Query: 2   GFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQ------PVPDHLIRQLQTIRAT 61
           GFTM LNLLLLVAMVATNILSLYHLSST    +S V         VPDHL+RQL TIRA 
Sbjct: 3   GFTMSLNLLLLVAMVATNILSLYHLSSTTNFFQSTVKSSQSSVPTVPDHLLRQLHTIRAA 62

Query: 62  INHLTRLHPTAAASASKTKRSI--------PSDLVLYSQFSPIASSCHGNPELLHRFMNY 121
           INHLT   P  + S S ++ ++        P +L++YS+ SPIAS+CH  P+LLH +MNY
Sbjct: 63  INHLTTHQPDKSTSTSTSRAAVSSSSSSTAPKELLIYSKLSPIASACHNYPDLLHEYMNY 122

Query: 122 TPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKY 181
           TPFS CPSD+DL E LILRGCHPLPRRRCF++TP+ PS S          PESN++W  Y
Sbjct: 123 TPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRTPRNPSDSK---------PESNVLWSYY 182

Query: 182 SCKGFGCLNRLNPNLGFDPSHEITK--FMTYKTELDLPIPQLLQIAKAANSVLRLGLDIG 241
           SCK F CL     +LGFD S E +K  F  YK+ELDLPI QLLQIAK+ANSVLRLG+D+G
Sbjct: 183 SCKSFDCLITKFSDLGFDLSLEKSKSQFSAYKSELDLPISQLLQIAKSANSVLRLGIDVG 242

Query: 242 GGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRC 301
           GGT +FAA +K  NVT++TTTMN  APY+E  A+RGLVPLHVPLQQRLP+FDGV+DLVRC
Sbjct: 243 GGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMRGLVPLHVPLQQRLPVFDGVVDLVRC 302

Query: 302 GHAVNRWIPVKSMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKW 361
           G AVNRWIPV  MEF F+D+DR+LR GGYLW D FFSK VDL+ VY+P+I KLGY+KVKW
Sbjct: 303 GRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRFFSKKVDLENVYAPMIGKLGYKKVKW 362

Query: 362 ATANKTDSGGLKNGEVYLTALLQKPV 372
           A ANK DS   K+GEV+LTALLQKPV
Sbjct: 363 AVANKADS---KHGEVFLTALLQKPV 376

BLAST of Spg035950 vs. ExPASy TrEMBL
Match: A0A1S3BA66 (uncharacterized protein LOC103487447 OS=Cucumis melo OX=3656 GN=LOC103487447 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 2.9e-210
Identity = 363/373 (97.32%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAASASKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPTAAASASKTKLSIPSDLVLYSQFSPIASSCHSNPELLHKFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNI+WGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNILWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYD+DRVLRVGGYLWFDHFFSKGVDLDK+YSPLITKLGYRKVKWATANKTDSGGLKNGE
Sbjct: 301 LFYDLDRVLRVGGYLWFDHFFSKGVDLDKLYSPLITKLGYRKVKWATANKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVP+
Sbjct: 361 VYLTALLQKPVPS 373

BLAST of Spg035950 vs. ExPASy TrEMBL
Match: A0A5A7UU83 (Methyltransf_29 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002990 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 2.9e-210
Identity = 363/373 (97.32%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAASASKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPTAAASASKTKLSIPSDLVLYSQFSPIASSCHSNPELLHKFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNI+WGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNILWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYD+DRVLRVGGYLWFDHFFSKGVDLDK+YSPLITKLGYRKVKWATANKTDSGGLKNGE
Sbjct: 301 LFYDLDRVLRVGGYLWFDHFFSKGVDLDKLYSPLITKLGYRKVKWATANKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVP+
Sbjct: 361 VYLTALLQKPVPS 373

BLAST of Spg035950 vs. ExPASy TrEMBL
Match: A0A0A0LXM4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G448980 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 4.7e-208
Identity = 361/373 (96.78%), Postives = 369/373 (98.93%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHPTAAASASKTK SIPSDLVLYSQFSPIASSCH NPELLH+FMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPTAAASASKTKLSIPSDLVLYSQFSPIASSCHTNPELLHKFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASS+PESNIIW KYSCKG GCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSLPESNIIWEKYSCKGLGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFMT+KTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMTFKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYD+DRVLRVGGYLWFDHFFSKGVDLDK+YSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDLDRVLRVGGYLWFDHFFSKGVDLDKLYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVPT 374
           VYLTALLQKPVP+
Sbjct: 361 VYLTALLQKPVPS 373

BLAST of Spg035950 vs. ExPASy TrEMBL
Match: A0A6J1EHZ7 (uncharacterized protein LOC111434341 OS=Cucurbita moschata OX=3662 GN=LOC111434341 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.0e-207
Identity = 359/372 (96.51%), Postives = 366/372 (98.39%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQS KSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSQKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHP AAAS SKTK SIPSDLVLYSQFSPIASSCH NPELLHRFMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPKAAASVSKTKVSIPSDLVLYSQFSPIASSCHSNPELLHRFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPF SS+PESNIIWGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFGSSLPESNIIWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFM++KTELDLPIPQLLQIAKAANSV+RLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMSFKTELDLPIPQLLQIAKAANSVVRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVP 373
           VYLTALLQKP+P
Sbjct: 361 VYLTALLQKPMP 372

BLAST of Spg035950 vs. ExPASy TrEMBL
Match: A0A6J1KL69 (uncharacterized protein LOC111495570 OS=Cucurbita maxima OX=3661 GN=LOC111495570 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.2e-206
Identity = 358/372 (96.24%), Postives = 365/372 (98.12%), Query Frame = 0

Query: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQPVPDHLIRQLQTIRATINHLT 60
           MGFTMGLNLLLLVAMVATNILSLYHLSSTLQS KSPVSQPVPDHLIRQLQTIRATINHLT
Sbjct: 1   MGFTMGLNLLLLVAMVATNILSLYHLSSTLQSQKSPVSQPVPDHLIRQLQTIRATINHLT 60

Query: 61  RLHPTAAASASKTKRSIPSDLVLYSQFSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLA 120
           RLHP AAAS SKTK SIPSDLVLYSQFSPIASSCH NPELLHRFMNYTPFSSCPSDSDLA
Sbjct: 61  RLHPKAAASISKTKVSIPSDLVLYSQFSPIASSCHSNPELLHRFMNYTPFSSCPSDSDLA 120

Query: 121 EALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKYSCKGFGCLNRLNP 180
           EALILRGCHPLPRRRCFA TPQKPSSSLPQNPF SS+PESNIIWGKYSCKGFGCLNRLNP
Sbjct: 121 EALILRGCHPLPRRRCFANTPQKPSSSLPQNPFGSSLPESNIIWGKYSCKGFGCLNRLNP 180

Query: 181 NLGFDPSHEITKFMTYKTELDLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNV 240
           NLGFDPSHEITKFM++KTELDLPIPQLLQIAKAANSV+RLGLDIGGGTATFAAR+KLYNV
Sbjct: 181 NLGFDPSHEITKFMSFKTELDLPIPQLLQIAKAANSVIRLGLDIGGGTATFAARMKLYNV 240

Query: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300
           TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF
Sbjct: 241 TMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEF 300

Query: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGE 360
           LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATA+KTDSGGLKNGE
Sbjct: 301 LFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKWATASKTDSGGLKNGE 360

Query: 361 VYLTALLQKPVP 373
           VYLTALLQKP+P
Sbjct: 361 VYLTALLQKPMP 372

BLAST of Spg035950 vs. TAIR 10
Match: AT1G29790.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 486.9 bits (1252), Expect = 1.5e-137
Identity = 249/386 (64.51%), Postives = 292/386 (75.65%), Query Frame = 0

Query: 2   GFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQ------PVPDHLIRQLQTIRAT 61
           GFTM LNLLLLVAMVATNILSLYHLSST    +S V         VPDHL+RQL TIRA 
Sbjct: 3   GFTMSLNLLLLVAMVATNILSLYHLSSTTNFFQSTVKSSQSSVPTVPDHLLRQLHTIRAA 62

Query: 62  INHLTRLHPTAAASASKTKRSI--------PSDLVLYSQFSPIASSCHGNPELLHRFMNY 121
           INHLT   P  + S S ++ ++        P +L++YS+ SPIAS+CH  P+LLH +MNY
Sbjct: 63  INHLTTHQPDKSTSTSTSRAAVSSSSSSTAPKELLIYSKLSPIASACHNYPDLLHEYMNY 122

Query: 122 TPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKY 181
           TPFS CPSD+DL E LILRGCHPLPRRRCF++TP+ PS S          PESN++W  Y
Sbjct: 123 TPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRTPRNPSDSK---------PESNVLWSYY 182

Query: 182 SCKGFGCLNRLNPNLGFDPSHEITK--FMTYKTELDLPIPQLLQIAKAANSVLRLGLDIG 241
           SCK F CL     +LGFD S E +K  F  YK+ELDLPI QLLQIAK+ANSVLRLG+D+G
Sbjct: 183 SCKSFDCLITKFSDLGFDLSLEKSKSQFSAYKSELDLPISQLLQIAKSANSVLRLGIDVG 242

Query: 242 GGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRC 301
           GGT +FAA +K  NVT++TTTMN  APY+E  A+RGLVPLHVPLQQRLP+FDGV+DLVRC
Sbjct: 243 GGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMRGLVPLHVPLQQRLPVFDGVVDLVRC 302

Query: 302 GHAVNRWIPVKSMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKW 361
           G AVNRWIPV  MEF F+D+DR+LR GGYLW D FFSK VDL+ VY+P+I KLGY+KVKW
Sbjct: 303 GRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRFFSKKVDLENVYAPMIGKLGYKKVKW 362

Query: 362 ATANKTDSGGLKNGEVYLTALLQKPV 372
           A ANK DS   K+GEV+LTALLQKPV
Sbjct: 363 AVANKADS---KHGEVFLTALLQKPV 376

BLAST of Spg035950 vs. TAIR 10
Match: AT1G29790.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 486.9 bits (1252), Expect = 1.5e-137
Identity = 249/386 (64.51%), Postives = 292/386 (75.65%), Query Frame = 0

Query: 2   GFTMGLNLLLLVAMVATNILSLYHLSSTLQSTKSPVSQ------PVPDHLIRQLQTIRAT 61
           GFTM LNLLLLVAMVATNILSLYHLSST    +S V         VPDHL+RQL TIRA 
Sbjct: 3   GFTMSLNLLLLVAMVATNILSLYHLSSTTNFFQSTVKSSQSSVPTVPDHLLRQLHTIRAA 62

Query: 62  INHLTRLHPTAAASASKTKRSI--------PSDLVLYSQFSPIASSCHGNPELLHRFMNY 121
           INHLT   P  + S S ++ ++        P +L++YS+ SPIAS+CH  P+LLH +MNY
Sbjct: 63  INHLTTHQPDKSTSTSTSRAAVSSSSSSTAPKELLIYSKLSPIASACHNYPDLLHEYMNY 122

Query: 122 TPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIPESNIIWGKY 181
           TPFS CPSD+DL E LILRGCHPLPRRRCF++TP+ PS S          PESN++W  Y
Sbjct: 123 TPFSLCPSDTDLVEKLILRGCHPLPRRRCFSRTPRNPSDSK---------PESNVLWSYY 182

Query: 182 SCKGFGCLNRLNPNLGFDPSHEITK--FMTYKTELDLPIPQLLQIAKAANSVLRLGLDIG 241
           SCK F CL     +LGFD S E +K  F  YK+ELDLPI QLLQIAK+ANSVLRLG+D+G
Sbjct: 183 SCKSFDCLITKFSDLGFDLSLEKSKSQFSAYKSELDLPISQLLQIAKSANSVLRLGIDVG 242

Query: 242 GGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQRLPIFDGVMDLVRC 301
           GGT +FAA +K  NVT++TTTMN  APY+E  A+RGLVPLHVPLQQRLP+FDGV+DLVRC
Sbjct: 243 GGTGSFAAAMKARNVTVLTTTMNFNAPYSEAVAMRGLVPLHVPLQQRLPVFDGVVDLVRC 302

Query: 302 GHAVNRWIPVKSMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKVYSPLITKLGYRKVKW 361
           G AVNRWIPV  MEF F+D+DR+LR GGYLW D FFSK VDL+ VY+P+I KLGY+KVKW
Sbjct: 303 GRAVNRWIPVTVMEFFFFDLDRILRGGGYLWLDRFFSKKVDLENVYAPMIGKLGYKKVKW 362

Query: 362 ATANKTDSGGLKNGEVYLTALLQKPV 372
           A ANK DS   K+GEV+LTALLQKPV
Sbjct: 363 AVANKADS---KHGEVFLTALLQKPV 376

BLAST of Spg035950 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 136/400 (34.00%), Postives = 214/400 (53.50%), Query Frame = 0

Query: 3   FTMGLNLLLLVAMVATNILSLYHLSSTLQS-TKSPVSQP----VPDHLIRQLQTIRATIN 62
           F+  +NLL+L ++V TN+ +LY  SS  QS T  P+       V  HL   L+ I ++ +
Sbjct: 21  FSSAINLLMLFSIVTTNLFALYAFSSRSQSHTPHPLHSNNVSLVSQHLSLILREIDSSHH 80

Query: 63  HLTRLHPTAAA--SASKTKRSIPSDLVLYSQ-----------------FSPIASSCHGNP 122
            LT++        S   +++ +P +L L+ Q                  + +  SC  + 
Sbjct: 81  TLTQMEKQIIGYESLDLSQQEVPQELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSL 140

Query: 123 ELLHRFMNYTPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIP 182
           +LL ++M+Y  F  CP D  LA+ LILR C PLPRRRC AKT  KP  +L  +     + 
Sbjct: 141 DLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCLAKTVHKPGLALFPDSLWRPVG 200

Query: 183 ESNIIWGKYSCKGFGCL--NRLNPN----LGFDPSHEITKFMTYKTELDLPIPQLLQIAK 242
            S++ W    CK F CL   +L+ +         SHE  +F+    + D  I  +L +  
Sbjct: 201 NSSVNWSGLGCKSFECLKGKKLSRDCVGCFDLATSHEKDRFVKVNGKTDFLIDDVLDL-- 260

Query: 243 AANSVLRLGLDIGGGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQR 302
             +  +R+G DI  G+ TFAAR+   NV +++ T+N+ AP++E  A RG+ PL + L QR
Sbjct: 261 -GDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQR 320

Query: 303 LPIFDGVMDLVRCGHAVNRWIPVK--SMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKV 362
           LP +D V DL+   + ++  +  K   +EFL +D+DR+L+ GG  W D+F+    +  +V
Sbjct: 321 LPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRV 380

Query: 363 YSPLITKLGYRKVKWATANKTDSGGLKNGEVYLTALLQKP 371
            + LI + GY+K+KW    KTD+      EV+L+A+LQKP
Sbjct: 381 LTRLIERFGYKKLKWVVGEKTDA------EVFLSAVLQKP 411

BLAST of Spg035950 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 136/400 (34.00%), Postives = 214/400 (53.50%), Query Frame = 0

Query: 3   FTMGLNLLLLVAMVATNILSLYHLSSTLQS-TKSPVSQP----VPDHLIRQLQTIRATIN 62
           F+  +NLL+L ++V TN+ +LY  SS  QS T  P+       V  HL   L+ I ++ +
Sbjct: 21  FSSAINLLMLFSIVTTNLFALYAFSSRSQSHTPHPLHSNNVSLVSQHLSLILREIDSSHH 80

Query: 63  HLTRLHPTAAA--SASKTKRSIPSDLVLYSQ-----------------FSPIASSCHGNP 122
            LT++        S   +++ +P +L L+ Q                  + +  SC  + 
Sbjct: 81  TLTQMEKQIIGYESLDLSQQEVPQELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSL 140

Query: 123 ELLHRFMNYTPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSSSLPQNPFASSIP 182
           +LL ++M+Y  F  CP D  LA+ LILR C PLPRRRC AKT  KP  +L  +     + 
Sbjct: 141 DLLSQYMSYNVFEKCPDDWSLAQKLILRACEPLPRRRCLAKTVHKPGLALFPDSLWRPVG 200

Query: 183 ESNIIWGKYSCKGFGCL--NRLNPN----LGFDPSHEITKFMTYKTELDLPIPQLLQIAK 242
            S++ W    CK F CL   +L+ +         SHE  +F+    + D  I  +L +  
Sbjct: 201 NSSVNWSGLGCKSFECLKGKKLSRDCVGCFDLATSHEKDRFVKVNGKTDFLIDDVLDL-- 260

Query: 243 AANSVLRLGLDIGGGTATFAARLKLYNVTMVTTTMNLGAPYNEVAALRGLVPLHVPLQQR 302
             +  +R+G DI  G+ TFAAR+   NV +++ T+N+ AP++E  A RG+ PL + L QR
Sbjct: 261 -GDGKIRIGFDISSGSGTFAARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQR 320

Query: 303 LPIFDGVMDLVRCGHAVNRWIPVK--SMEFLFYDVDRVLRVGGYLWFDHFFSKGVDLDKV 362
           LP +D V DL+   + ++  +  K   +EFL +D+DR+L+ GG  W D+F+    +  +V
Sbjct: 321 LPFYDNVFDLIHASNGLDLAVSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRV 380

Query: 363 YSPLITKLGYRKVKWATANKTDSGGLKNGEVYLTALLQKP 371
            + LI + GY+K+KW    KTD+      EV+L+A+LQKP
Sbjct: 381 LTRLIERFGYKKLKWVVGEKTDA------EVFLSAVLQKP 411

BLAST of Spg035950 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-57
Identity = 121/293 (41.30%), Postives = 169/293 (57.68%), Query Frame = 0

Query: 87  FSPIASSCHGNPELLHRFMNYTPFSSCPSDSDLAEALILRGCHPLPRRRCFAKTPQKPSS 146
           ++ I  +C      L ++M+Y   + C  D +LA+ L+L GC PLPRRRC  +       
Sbjct: 177 YNTIGHACALMKMELEKYMDYDVGAYCDDDWNLAQKLMLNGCDPLPRRRCLTRASMTYQK 236

Query: 147 SLPQNPFASSIPES-NIIWGKYSCKGFGCLNRLNPNLG-------FDPSHEITKFMTYKT 206
             P N     +P+  N+ WG Y C+ F CL+  NP  G       F+   E  K++   +
Sbjct: 237 PYPINESLWKLPDDRNVRWGNYQCRNFACLSSKNPKRGYTKCSGCFEMEKEKDKWVKNSS 296

Query: 207 EL-DLPIPQLLQIAKAANSVLRLGLDIGGGTATFAARLKLYNVTMVTTTMNLGAPYNEVA 266
            L D  I  +L++     S +R+GLD G GT TFAAR++  NVT+VTT +NLGAP+NE+ 
Sbjct: 297 LLVDFMIEDVLRVKP---SEIRIGLDYGVGTGTFAARMREKNVTIVTTALNLGAPFNEMI 356

Query: 267 ALRGLVPLHVPLQQRLPIFDGVMDLVRCGHAVNRWIPVKSMEFLFYDVDRVLRVGGYLWF 326
           ALRGL+PL++ L QRLP FD  MD++     ++ WI +  M+F+ YD DRVLR GG LW 
Sbjct: 357 ALRGLIPLYISLNQRLPFFDNTMDMIHTTGLMDGWIDLLLMDFVLYDWDRVLRPGGLLWI 416

Query: 327 DHFFSKGVDLDKVYSPLITKLGYRKVKWATANKTDSGGLKNGEVYLTALLQKP 371
           D FF K  DLD  Y  +  +  Y+K KWA + K+        EVYL+ALL+KP
Sbjct: 417 DRFFCKKKDLDD-YMYMFLQFRYKKHKWAISPKS------KDEVYLSALLEKP 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008443998.16.1e-21097.32PREDICTED: uncharacterized protein LOC103487447 [Cucumis melo] >XP_008444006.1 P... [more]
XP_038895531.17.9e-21097.32uncharacterized protein LOC120083747 [Benincasa hispida] >XP_038895532.1 unchara... [more]
XP_004147405.19.7e-20896.78uncharacterized protein LOC101208739 [Cucumis sativus] >XP_004147406.1 uncharact... [more]
XP_023520344.16.3e-20796.51uncharacterized protein LOC111783659 [Cucurbita pepo subsp. pepo][more]
XP_022927546.16.3e-20796.51uncharacterized protein LOC111434341 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8RWB72.1e-13664.51Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE... [more]
Match NameE-valueIdentityDescription
A0A1S3BA662.9e-21097.32uncharacterized protein LOC103487447 OS=Cucumis melo OX=3656 GN=LOC103487447 PE=... [more]
A0A5A7UU832.9e-21097.32Methyltransf_29 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0LXM44.7e-20896.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G448980 PE=3 SV=1[more]
A0A6J1EHZ73.0e-20796.51uncharacterized protein LOC111434341 OS=Cucurbita moschata OX=3662 GN=LOC1114343... [more]
A0A6J1KL691.2e-20696.24uncharacterized protein LOC111495570 OS=Cucurbita maxima OX=3661 GN=LOC111495570... [more]
Match NameE-valueIdentityDescription
AT1G29790.11.5e-13764.51S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G29790.21.5e-13764.51S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.11.0e-5834.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.21.0e-5834.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G05390.11.2e-5741.30FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 191..359
e-value: 2.3E-5
score: 25.7
NoneNo IPR availablePANTHERPTHR44067:SF5EXPRESSED PROTEINcoord: 1..371
NoneNo IPR availablePANTHERPTHR44067S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATEDcoord: 1..371
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 206..316
e-value: 3.4E-6
score: 25.9
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 222..328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg035950.1Spg035950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity