Spg035314 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035314 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 768.8 bits (1984), Expect = 3.3e-218
Identity = 401/586 (68.43%), Postives = 469/586 (80.03%), Query Frame = 0

Query: 12  VVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLT 71
           VV+Y   PIGRQL YLFFY RNIKDLEKQL++L+  RERVQ LV E RSN+Y+IYD V T
Sbjct: 11  VVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSNAYEIYDEVST 70

Query: 72  W-LDEVDVELLETLRQNE------ASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKF 131
           W L EVDVEL    RQ E      +SN+ C N +Q H+L+RK+KKR++ I +LI++G KF
Sbjct: 71  WLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDILQLIEEGKKF 130

Query: 132 HQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTK 191
            +  VG  APLP T   TLPADYQ+LESRTLMAE+IKDAL++PNVNKVGVCGM GVGKTK
Sbjct: 131 EK--VGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCGMAGVGKTK 190

Query: 192 LLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAALIQT 251
           LL E+KKLVLEN LF RVI V VG S GV+EIQD+IGG LNM L+  A +KE RA  ++ 
Sbjct: 191 LLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKEGRAPFLRN 250

Query: 252 KIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTER 311
           K+VE+KD ILIMLDDLW EYDLEKE GIPCR SES KE CKILMT RSRDI TN+MNT++
Sbjct: 251 KLVEMKDKILIMLDDLWNEYDLEKEVGIPCR-SESSKEGCKILMTSRSRDILTNKMNTKK 310

Query: 312 LFEVNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGKKVQV 371
            F+VN LS++ESWKFF+AIVGEF +   + IA++VVK+CGGLPIALKI+AKALKGK++ +
Sbjct: 311 CFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKALKGKEIHI 370

Query: 372 WKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISVEDL 431
           WKDA +K + HVL+ I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I V+DL
Sbjct: 371 WKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHNICVKDL 430

Query: 432 QMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIA 491
           QMYAM MGLLNDINNWE  KNRVI LVDDLKSSYLLLESN  DNYV MHDVVRDVA YIA
Sbjct: 431 QMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIA 490

Query: 492 SKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQR 551
           S  +KM SL+YGCGQ+EW EEDR GSYNAIFV+C DF +LP   EFP LQLL+L   +  
Sbjct: 491 SNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFF 550

Query: 552 KE-KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
            E +AIQIP  FFEGME LRVLDMT MCFEPS+TS+THSL+NL+TL
Sbjct: 551 GEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTL 593

BLAST of Spg035314 vs. NCBI nr
Match: XP_022155050.1 (disease resistance protein RPS5-like [Momordica charantia])

HSP 1 Score: 752.7 bits (1942), Expect = 2.4e-213
Identity = 390/590 (66.10%), Postives = 470/590 (79.66%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           IV  V +Y   PIGRQLGYLFFY +NIKD+EKQL++L+  RE+VQ LV E RSN+Y+IYD
Sbjct: 7   IVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAYEIYD 66

Query: 68  HVLTW-LDEVDVELLETLRQN------EASNMWCFNCIQWHRLARKTKKRLEYIHKLIDD 127
            V TW L EVDVEL     +N      + SN  C N +Q H+L+R++KKR++ I ++I+ 
Sbjct: 67  EVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILEIIEK 126

Query: 128 GNKFHQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGV 187
           G KF +  VG  APLP T   TLPADYQILESRTL+AE+IKD+LADPNVNKVGVCGMGGV
Sbjct: 127 GKKFDK--VGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMGGV 186

Query: 188 GKTKLLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAA 247
           GKTKLL EVKKLVLEN LF RVI V +G S GV+EIQD+IGG LNM LN  A +KE RA 
Sbjct: 187 GKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGRAP 246

Query: 248 LIQTKIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQM 307
            ++ K+VE+KD+ILIMLDDLW EYDL+KE GIPCR S S KE CKILMT RSRDI TN+M
Sbjct: 247 FLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCR-SRSSKEGCKILMTSRSRDILTNRM 306

Query: 308 NTERLFEVNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGK 367
           + E+ FEVN LS+EESWKFF+AIVGEF ++  + +AR+VVK+CGGLPIALKI+AKALKGK
Sbjct: 307 DAEKCFEVNSLSEEESWKFFIAIVGEFDKNRIEHLARNVVKKCGGLPIALKIIAKALKGK 366

Query: 368 KVQVWKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYIS 427
            V++WKDAL+K +  V + I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I 
Sbjct: 367 DVRIWKDALEKLEKRVSVKIKGVSDQLYSCLKLSYDRIEDEETRLLFLLCSVFPDDHKIH 426

Query: 428 VEDLQMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVA 487
           V+DLQMYAM MGLLNDI+NWE ++N V++LVD LKSS LLLESN  DNYV MHDVVRDVA
Sbjct: 427 VKDLQMYAMGMGLLNDIDNWETSRNMVVELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 486

Query: 488 IYIASKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRN 547
            YIA+K NKM SL+YG GQ+EWRE++R GSYN IF++C DF NLPP +EFP LQ+L+LR 
Sbjct: 487 KYIATKENKMSSLSYGRGQSEWREKERCGSYNVIFIDCVDFPNLPPNMEFPDLQMLILRI 546

Query: 548 ETQRKE-KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           ++   E +AIQIP+  FEGM+ LRVLDMT MCFEPSKTS THSL+NL+TL
Sbjct: 547 QSFFGEGRAIQIPEALFEGMKKLRVLDMTKMCFEPSKTSFTHSLKNLQTL 593

BLAST of Spg035314 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 679.9 bits (1753), Expect = 2.0e-191
Identity = 365/583 (62.61%), Postives = 429/583 (73.58%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           +V  +  Y   PI  QL YLF Y+ NIK++EK+L  L      VQ LV + +     I+D
Sbjct: 76  VVGVLADYTIKPILHQLDYLFHYKGNIKEVEKKLGILRTTERTVQILVNQDKGKGNAIFD 135

Query: 68  HVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQY 127
            V  WLD V   +L+  +QNEA N  CFNC+Q H+L+RK KKRLE I++LI++G +F++ 
Sbjct: 136 GVTNWLDNVK-NVLDMAQQNEAPNPSCFNCVQRHQLSRKAKKRLEDINELINEGRQFNKD 195

Query: 128 NVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLG 187
           NVG   P P T+  TLPADYQ  ESRT MAE+IKDALA+PNVNKVGVCGMGGVGKT LL 
Sbjct: 196 NVGYPVPSPDTSSSTLPADYQFFESRTSMAEKIKDALANPNVNKVGVCGMGGVGKTTLLK 255

Query: 188 EVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMK-LNANSKEVRAALIQTKIVE 247
           EVKKLVLEN LF RVI V VG SK VI IQ+EI   LNM+ LN   +EVR + +QT I E
Sbjct: 256 EVKKLVLENKLFDRVIQVEVGQSKSVIHIQEEIRAKLNMEYLNMEFEEVRTSRLQTHIAE 315

Query: 248 IKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEV 307
            K+NIL MLDDLWKEYD+EKEFGIP R S+SR E CKILMT R RD+FTNQMN E+LFEV
Sbjct: 316 KKENILFMLDDLWKEYDVEKEFGIPYR-SDSRIEGCKILMTSRRRDLFTNQMNIEKLFEV 375

Query: 308 NPLSDEESWKFFVAIVG-EFVQDG-TKQIARDVVKECGGLPIALKILAKALKGKKVQVWK 367
           N L+ EESWKFF  I+G EFV+DG  KQIA+ V+ ECGGLPIALKIL K LKG++V++WK
Sbjct: 376 NSLTKEESWKFFSNIIGDEFVEDGYMKQIAKYVINECGGLPIALKILGKTLKGRRVEIWK 435

Query: 368 DALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMY 427
           DALKK KN V++ I GV+DQLY+CL+ SYDS+E EAK L LLC VFPDDH I V+DLQ Y
Sbjct: 436 DALKKLKNPVVVDIHGVSDQLYSCLQFSYDSIENEAKLLLLLCSVFPDDHRIYVDDLQKY 495

Query: 428 AMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQW-DNYVLMHDVVRDVAIYIASK 487
           AM MGLLN  N WEDAKNRVIKLVDDLKSSYLLL   +W DNYV MHDVV D A YIASK
Sbjct: 496 AMGMGLLNQTNTWEDAKNRVIKLVDDLKSSYLLLAPPEWDDNYVKMHDVVHDFAKYIASK 555

Query: 488 INKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKE 547
           I+KM S TYG GQ  W E++R GSYNAI+V+   FLNLPPKLEFP LQL +L +      
Sbjct: 556 IDKMTSFTYGNGQRAWEEDNRHGSYNAIYVDPTTFLNLPPKLEFPNLQLFILISNGLGPR 615

Query: 548 KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
             IQ  +  FEGM+ LRVLD+    FEP   SL +SL+NLR L
Sbjct: 616 PTIQNLETVFEGMKELRVLDVRMWYFEP---SLIYSLKNLRVL 653

BLAST of Spg035314 vs. NCBI nr
Match: XP_038887227.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 678.7 bits (1750), Expect = 4.4e-191
Identity = 354/583 (60.72%), Postives = 435/583 (74.61%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           +V  VV+Y   PI  QLGYLF Y  N+K L+++ K LE  R+ VQ LV++ +     I+D
Sbjct: 4   VVGVVVEYTIRPILHQLGYLFRYNGNVKKLQEKHKTLETTRDTVQILVSQDKGKGNAIFD 63

Query: 68  HVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQY 127
            V  WLD V  E+    +QN+  N  CFN ++ HRL+RK+KK +E I +LI++G +F++ 
Sbjct: 64  EVTKWLDNVK-EVSNMTKQNKELNPSCFNFVKRHRLSRKSKKMVEDIAELINEGRRFNKD 123

Query: 128 NVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLG 187
            +G   P   TNCPT+P DYQI+ESRTL+ E IKDALA+PNVNKVGVCGMGGVGKT LL 
Sbjct: 124 RIGYPVPSSDTNCPTIPTDYQIIESRTLLVENIKDALANPNVNKVGVCGMGGVGKTSLLN 183

Query: 188 EVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLNANSKEVRAALIQTKIVEI 247
           EVKKLVLENNLF RVI V VG SK VI+IQ+EI G LNM+      ++ A+L+QT I+E 
Sbjct: 184 EVKKLVLENNLFDRVIQVEVGQSKSVIDIQEEIRGALNMQCG----KITASLLQTHIIER 243

Query: 248 KDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVN 307
           K+NIL MLDDLWK+YDLEKEFGIPCR SESR E CKILMT RSR   TNQMN E+LFEVN
Sbjct: 244 KENILFMLDDLWKDYDLEKEFGIPCR-SESRTEGCKILMTSRSRHTLTNQMNIEKLFEVN 303

Query: 308 PLSDEESWKFFVAIVGEFVQDG-TKQIARDVVKECGGLPIALKILAKALKGKKVQVWKDA 367
           P++ +ESW FF AIVGE+V+DG  +QIA+DVV ECGGLPIALK++AKALKGK+V++WKDA
Sbjct: 304 PVTKKESWMFFTAIVGEYVEDGYIQQIAKDVVNECGGLPIALKVIAKALKGKRVEIWKDA 363

Query: 368 LKKFKNHVLMGIPGVNDQLYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMYAM 427
           LKK KN V++ I GV+D+LY+CL+ SYDS+E EAK + LLC VFPDDH I V+DLQMYA+
Sbjct: 364 LKKLKNPVVVNISGVSDRLYSCLQFSYDSIEDEAKLVLLLCSVFPDDHRIYVKDLQMYAV 423

Query: 428 SMGLLNDINNWEDAKNRVIKLVDDLKSSYLLL--ESNQWDNYVLMHDVVRDVAIYIASKI 487
            MG LN IN WEDA+NRVIKLVDDLKSS LLL  +  + + YV +HDV+ D   YIASK 
Sbjct: 424 GMGFLNHINTWEDARNRVIKLVDDLKSSNLLLAPDEEEGEEYVKIHDVIHDFGKYIASKT 483

Query: 488 NKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEK 547
           +KM SLTY  GQ E +E DR G+YNAI++NC +FLN P KLE P LQLL+LRN      K
Sbjct: 484 DKMSSLTYRSGQKELQENDRHGTYNAIYLNCSEFLNYPQKLELPNLQLLILRN-VLLDYK 543

Query: 548 AIQIPDGFFEGMENLRVLDM-TNMCFEPSKTSLTHSLRNLRTL 587
            I IP  FFEGM  +RVLD+      +P  TSLT SL+NL+TL
Sbjct: 544 VIPIPVAFFEGMNGVRVLDVRVAHLGQPQLTSLTQSLKNLQTL 579

BLAST of Spg035314 vs. NCBI nr
Match: XP_022155051.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 582.8 bits (1501), Expect = 3.3e-162
Identity = 326/583 (55.92%), Postives = 392/583 (67.24%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           +V  + +Y   PIGRQLGYLFFY RNIKDLEKQL++L+   E+VQ LV E+         
Sbjct: 7   VVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTGEKVQILVNEE--------- 66

Query: 68  HVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQY 127
                                                       E IH          + 
Sbjct: 67  --------------------------------------------EVIHM-----KSMTKS 126

Query: 128 NVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLG 187
             GC    P++                             NVNKVGV GMGGVGKTK L 
Sbjct: 127 RHGC---WPKS-----------------------------NVNKVGVYGMGGVGKTKWLD 186

Query: 188 EVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAALIQTKIV 247
           EVKKLVLEN LF RVI V +G S GV+EIQD+IGG LNM LN  A +KE RA  +Q K+V
Sbjct: 187 EVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLV 246

Query: 248 EIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFE 307
           E+++NILI+LDDLW EYDL+KE GIPCR SES KE CKILMT RSR I TN MNTE+ F+
Sbjct: 247 EMRENILILLDDLWNEYDLQKEVGIPCR-SESSKEGCKILMTSRSRHILTNNMNTEKCFK 306

Query: 308 VNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGKKVQVWKD 367
           VN LS+EESWKFF+ IVGEF ++  + +A++VVK+CGGLPIALKI+AKALKGK++++WK+
Sbjct: 307 VNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGKELRIWKN 366

Query: 368 ALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISVEDLQMY 427
           AL+K +  V + I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I  +DLQMY
Sbjct: 367 ALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMY 426

Query: 428 AMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKI 487
           AM MGLLN+++NWE  +N VI+LVD LKSS LLLESN  DNYV MHDVVRDVA YIAS  
Sbjct: 427 AMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNE 486

Query: 488 NKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKE- 547
           N M SL+YGCGQ+EWRE+DR GSYNAIFV+C DF NLP  LEFP LQ+L+LR +T   E 
Sbjct: 487 NNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEG 498

Query: 548 KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           +AIQIPD FFEGM+ LRVLDMT M FE S+TSLT+SL+NL+TL
Sbjct: 547 QAIQIPDTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTL 498

BLAST of Spg035314 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.4e-54
Identity = 175/564 (31.03%), Postives = 282/564 (50.00%), Query Frame = 0

Query: 35  KDLEKQLKD-LELIRERVQ--SLVTEQRSNSYKIYDHVLTWLDEVDVELLETLRQNEASN 94
           K LE++L+  L  + E V    L+ E+RS+          WL + DVE+LE +++ E   
Sbjct: 35  KSLERKLRIWLRKVEENVPLGELILEKRSS-------CAIWLSDKDVEILEKVKRLEEQG 94

Query: 95  MWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQTNCPTLPADYQILE 154
                 I  ++ +R+  +R+         G  FH          PQ             +
Sbjct: 95  QDLIKKISVNKSSREIVERVL--------GPSFH----------PQ-------------K 154

Query: 155 SRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLE---NNLFHRVIVVIVG 214
           +   M +++KD L   NV K+GV GMGGVGKT L+  +   +L+      F  VI V V 
Sbjct: 155 TALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVS 214

Query: 215 TSKGVIEIQDEIGGILNMKLNANSKEVRAALIQTKIVEIKDNILIMLDDLWKEYDLEKEF 274
               +  +Q +I   L  +            I  +++++K N L++LDD+W   DL+ + 
Sbjct: 215 KDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLK-NFLLILDDVWHPIDLD-QL 274

Query: 275 GIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWKFFVAIVGEFV-Q 334
           GIP   +  R +  K+++T R  ++   QM T    +V  L ++E+W+ F   VGE    
Sbjct: 275 GIPL--ALERSKDSKVVLTSRRLEV-CQQMMTNENIKVACLQEKEAWELFCHNVGEVANS 334

Query: 335 DGTKQIARDVVKECGGLPIALKILAKALKGK-KVQVWKDALKKFKNHVLMGIPGVN--DQ 394
           D  K IA+DV  EC GLP+A+  + + L+GK +V+VWK  L   K       P ++  ++
Sbjct: 335 DNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR----SAPSIDTEEK 394

Query: 395 LYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMYAMSMGLLNDINNWEDAKNRV 454
           ++  LK+SYD ++   KS FL C +FP+D+ I V +L MY ++ GLL+  +++ED  N  
Sbjct: 395 IFGTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEG 454

Query: 455 IKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAI-YIASKINKMPSLTY-GCGQNEWREE 514
           + LV+ LK S LL + +  D  V MHDVVRD AI +++S+     SL   G G  E+ ++
Sbjct: 455 VTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQD 514

Query: 515 DRRGSYNAIFVNCEDFLNLPPK-LEFPTLQLLVLRNETQRKEKAIQIPDGFFEGMENLRV 574
               S   + +       LP   +E     +L+L+  +  KE    +P+GF +   NLR+
Sbjct: 515 KFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKE----VPNGFLQAFPNLRI 546

Query: 575 LDMTNMCFE--PSKTSLTHSLRNL 584
           LD++ +     P   S  HSLR+L
Sbjct: 575 LDLSGVRIRTLPDSFSNLHSLRSL 546

BLAST of Spg035314 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 3.2e-51
Identity = 163/549 (29.69%), Postives = 277/549 (50.46%), Query Frame = 0

Query: 30  YQRNIKDLEKQLKDLELIR----ERVQSLVTEQRSNSYKIYDHVLTWLDEVDVELLETLR 89
           ++ N+K L + L+ L  ++    E  ++L+T+ +    K+    + W  E + E++   R
Sbjct: 29  FKSNVKALNESLERLTELKGNMSEDHETLLTKDKPLRLKL----MRWQREAE-EVISKAR 88

Query: 90  QNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQTNCPTLPA 149
                 + C   ++  R++RK  K L+ +  L  DG +F       S P    + P +  
Sbjct: 89  LKLEERVSCGMSLR-PRMSRKLVKILDEVKMLEKDGIEFVDMLSVESTPERVEHVPGVSV 148

Query: 150 DYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLENNL---FHRV 209
            +Q + S   M  +I+D L      K+GV GMGGVGKT L+  +   + E      F  V
Sbjct: 149 VHQTMASN--MLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLV 208

Query: 210 IVVIVGTSKGVIEIQDEIGGILNMKLNANSKEVRAALIQTKIVEIKDNILIMLDDLWKEY 269
           I VIV       E+Q +I   L++       E + A      +  +   L++LDD+WK  
Sbjct: 209 IFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLARRIYVGLMKERKFLLILDDVWKPI 268

Query: 270 DLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWKFFVAIV 329
           DL+   GIP R  E++    K+++T R  ++    M T+    V+ L +E++W+ F    
Sbjct: 269 DLDL-LGIP-RTEENKGS--KVILTSRFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNA 328

Query: 330 GEFVQ-DGTKQIARDVVKECGGLPIALKILAKALKGKK-VQVWKDALKKFKNHVLMGIPG 389
           G+ V+ D  ++IA+ V +ECGGLP+A+  +  A++GKK V++W   L K    V   I  
Sbjct: 329 GDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSV-PWIKS 388

Query: 390 VNDQLYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMYAMSMGLLNDINNWEDA 449
           + ++++  LK+SYD +E +AK  FLLC +FP+D+ I V ++  Y M+ G + ++ + ED+
Sbjct: 389 IEEKIFQPLKLSYDFLEDKAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDS 448

Query: 450 KNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYI--ASKINKMPSLTYGCGQNE 509
            N  I  V+ LK  Y LLE     + V MHDVVRD AI+I  +S+ +    +  G G  +
Sbjct: 449 MNEGITTVESLK-DYCLLEDGDRRDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQD 508

Query: 510 WREEDRRGSYNAIFVNCEDFLNLPPKLE--FPTLQLLVLRNETQRKEKAIQIPDGFFEGM 566
            R++    S   + +      +LP  +E       +L+L+     KE    +P GF +  
Sbjct: 509 IRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGNFLLKE----VPIGFLQAF 558

BLAST of Spg035314 vs. ExPASy Swiss-Prot
Match: Q940K0 (Disease resistance protein UNI OS=Arabidopsis thaliana OX=3702 GN=UNI PE=1 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 1.1e-43
Identity = 156/561 (27.81%), Postives = 269/561 (47.95%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLTWLD---EVDVE--- 85
           Y+   ++N++ L+++++DL  I+  VQ+ V    +   +  + V  WLD    VD+E   
Sbjct: 26  YIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEAVQVWLDRVNSVDIECKD 85

Query: 86  LLETLRQNEASNMWCFNCIQW----HRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPL 145
           LL            C  C ++    ++  +K    LE + KL  +GN F +     S P 
Sbjct: 86  LLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLNSEGN-FDE----VSQPP 145

Query: 146 PQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLE 205
           P++     P    I   +  M E+  + L +  V  +G+ GMGGVGKT L  ++     E
Sbjct: 146 PRSEVEERPTQPTI--GQEDMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAE 205

Query: 206 -NNLFHRVIVVIVGTSKGVIEIQDEIGGILNM--KLNANSKEVRAALIQTKIVEIKDNIL 265
               F  VI ++V     + ++Q++I   L++   L  N  E   A    ++++ K   +
Sbjct: 206 IGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGK-RFV 265

Query: 266 IMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDE 325
           +MLDD+W++ DLE   GIP     S   +CK+  T RSR++   +M   +  +VN L  E
Sbjct: 266 LMLDDIWEKVDLE-AIGIP---YPSEVNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPE 325

Query: 326 ESWKFFVAIVGEFVQDGTK---QIARDVVKECGGLPIALKILAKALKGK-KVQVWKDALK 385
           ++W+ F   VG+          ++AR+V ++C GLP+AL ++ + +  K  VQ W+ A+ 
Sbjct: 326 DAWELFKNKVGDNTLSSDPVIVELAREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIH 385

Query: 386 KFKNHVLMGIPGVNDQLYTCLKISYDSVEGE-AKSLFLLCGVFPDDHYISVEDLQMYAMS 445
            F N        + +++   LK SYDS+  E  KS FL C +FP+D  I  E L  Y + 
Sbjct: 386 VF-NTSAAEFSDMQNKILPILKYSYDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWIC 445

Query: 446 MGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKINKM 505
            G + +    + A+N+   ++  L  + LL +   +  Y +MHDVVR++A++IAS   K 
Sbjct: 446 EGFIGEDQVIKRARNKGYAMLGTLTRANLLTKVGTY--YCVMHDVVREMALWIASDFGKQ 505

Query: 506 PS---LTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEK 565
                +  G G +E  +    G+   + +   D   +  + +   L  L L++      K
Sbjct: 506 KENFVVQAGVGLHEIPKVKDWGAVRKMSLMDNDIEEITCESKCSELTTLFLQS-----NK 565

BLAST of Spg035314 vs. ExPASy Swiss-Prot
Match: O64973 (Disease resistance protein RPS5 OS=Arabidopsis thaliana OX=3702 GN=RPS5 PE=1 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 2.7e-42
Identity = 154/554 (27.80%), Postives = 267/554 (48.19%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIR-ERVQSLVTEQRSNSYKIYDHVLTWLDEVDV---ELL 85
           Y+    +N+  L+K ++ L+  + + ++ L TE+ +   +    V  WL  V +   +  
Sbjct: 28  YIHNLSKNLASLQKAMRMLKARQYDVIRRLETEEFTGRQQRLSQVQVWLTSVLIIQNQFN 87

Query: 86  ETLRQNEASNM---WCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQT 145
           + LR NE        C  C +  +L+ +  KR+  + K ++  +    ++V  S   P  
Sbjct: 88  DLLRSNEVELQRLCLCGFCSKDLKLSYRYGKRVIMMLKEVESLSSQGFFDV-VSEATPFA 147

Query: 146 NCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEV-KKLVLENN 205
           +   +P    I+  + +M E+  + L +     +G+ GMGGVGKT LL ++  K    ++
Sbjct: 148 DVDEIPFQPTIV-GQEIMLEKAWNRLMEDGSGILGLYGMGGVGKTTLLTKINNKFSKIDD 207

Query: 206 LFHRVIVVIVGTSKGVIEIQDEIGGILNM-KLNANSKEVRAALIQTKIVEIKDNILIMLD 265
            F  VI V+V  S  V +IQ +I   + +  +  + K      +    V  +   +++LD
Sbjct: 208 RFDVVIWVVVSRSSTVRKIQRDIAEKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVLLLD 267

Query: 266 DLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWK 325
           D+W++ +L K  G+P     S+   CK+  T RSRD+   +M  +   EV+ L  EESW 
Sbjct: 268 DIWEKVNL-KAVGVP---YPSKDNGCKVAFTTRSRDV-CGRMGVDDPMEVSCLQPEESWD 327

Query: 326 FFVAIVGEFVQDGTKQI---ARDVVKECGGLPIALKILAKALKGKK-VQVWKDALKKFKN 385
            F   VG+        I   AR V ++C GLP+AL ++ +A+  K+ V  W  A+    +
Sbjct: 328 LFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDVLTS 387

Query: 386 HVLMGIPGVNDQLYTCLKISYDSVEGE-AKSLFLLCGVFPDDHYISVEDLQMYAMSMGLL 445
             +    G+ D++   LK SYD++ GE  KS FL C +FP+D+ I  E L  Y +S G +
Sbjct: 388 SAI-DFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISEGFI 447

Query: 446 NDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKINKMPSLT 505
           N+    E   N+  +++  L  + LLLE  +  + V MHDVVR++A++I+S + K     
Sbjct: 448 NEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQKEKC 507

Query: 506 YGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEKAIQIPDG 565
                   RE  +   +N   V     +N   +  F + +   L     +K   ++I   
Sbjct: 508 IVRAGVGLREVPKVKDWNT--VRKISLMNNEIEEIFDSHECAALTTLFLQKNDVVKISAE 567

BLAST of Spg035314 vs. ExPASy Swiss-Prot
Match: Q9FG91 (Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN=At5g43730 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 1.1e-40
Identity = 164/567 (28.92%), Postives = 267/567 (47.09%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLTWLDEVDV------E 85
           Y+   + N+  L+K +++L+  R+ + + V+ +     +    V  WL  V +      +
Sbjct: 27  YIHLMESNLDALQKTMEELKNGRDDLLARVSIEEDKGLQRLALVNGWLSRVQIVESEFKD 86

Query: 86  LLETLRQNEASNMWCF-----NCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAP 145
           LLE +   E   +  F     +CI  +    K  K LE + +L+   N    + V     
Sbjct: 87  LLEAM-SIETGRLCLFGYCSEDCISSYNYGGKVMKNLEEVKELLSKKN----FEVVAQKI 146

Query: 146 LPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEV-KKLV 205
           +P+          Q       M     ++L D  +  +G+ GMGG+GKT LL  +  K V
Sbjct: 147 IPKAE----KKHIQTTVGLDTMVGIAWESLIDDEIRTLGLYGMGGIGKTTLLESLNNKFV 206

Query: 206 LENNLFHRVIVVIVGTSKGVIEIQDEIGGIL--NMKLNANSKEVRAALIQTKIVEIKDNI 265
              + F  VI V+V     +  IQD+I G L  + +    ++  +A+LI   +   K   
Sbjct: 207 ELESEFDVVIWVVVSKDFQLEGIQDQILGRLRPDKEWERETESKKASLINNNLKRKK--F 266

Query: 266 LIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSD 325
           +++LDDLW E DL K  G+P     SR+   KI+ T RS+++    M  ++  +V+ LS 
Sbjct: 267 VLLLDDLWSEVDLIK-IGVP---PPSRENGSKIVFTTRSKEV-CKHMKADKQIKVDCLSP 326

Query: 326 EESWKFFVAIVGEFVQDGTKQI---ARDVVKECGGLPIALKILAKALKGKK-VQVWKDAL 385
           +E+W+ F   VG+ +    + I   AR V  +C GLP+AL ++ KA+  K+ VQ W+ A+
Sbjct: 327 DEAWELFRLTVGDIILRSHQDIPALARIVAAKCHGLPLALNVIGKAMVCKETVQEWRHAI 386

Query: 386 KKFKNHVLMGIPGVNDQLYTCLKISYDSVE-GEAKSLFLLCGVFPDDHYISVEDLQMYAM 445
               N      PG+ +++   LK SYDS++ GE K  FL C +FP+D  I  + L  Y +
Sbjct: 387 NVL-NSPGHKFPGMEERILPILKFSYDSLKNGEIKLCFLYCSLFPEDFEIEKDKLIEYWI 446

Query: 446 SMGLLNDINNWED-AKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKI- 505
             G +N  N +ED   N+   ++  L  ++LL+E    D  V MHDV+R++A++I S   
Sbjct: 447 CEGYINP-NRYEDGGTNQGYDIIGLLVRAHLLIECELTDK-VKMHDVIREMALWINSDFG 506

Query: 506 NKMPSLTYGCG------QNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNE 565
           N+  ++    G       N+   E  R   + I    E     P     P L  L+L   
Sbjct: 507 NQQETICVKSGAHVRLIPNDISWEIVR-QMSLISTQVEKIACSP---NCPNLSTLLL--- 565

BLAST of Spg035314 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.6e-218
Identity = 401/586 (68.43%), Postives = 469/586 (80.03%), Query Frame = 0

Query: 12  VVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLT 71
           VV+Y   PIGRQL YLFFY RNIKDLEKQL++L+  RERVQ LV E RSN+Y+IYD V T
Sbjct: 11  VVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSNAYEIYDEVST 70

Query: 72  W-LDEVDVELLETLRQNE------ASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKF 131
           W L EVDVEL    RQ E      +SN+ C N +Q H+L+RK+KKR++ I +LI++G KF
Sbjct: 71  WLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDILQLIEEGKKF 130

Query: 132 HQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTK 191
            +  VG  APLP T   TLPADYQ+LESRTLMAE+IKDAL++PNVNKVGVCGM GVGKTK
Sbjct: 131 EK--VGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCGMAGVGKTK 190

Query: 192 LLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAALIQT 251
           LL E+KKLVLEN LF RVI V VG S GV+EIQD+IGG LNM L+  A +KE RA  ++ 
Sbjct: 191 LLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKEGRAPFLRN 250

Query: 252 KIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTER 311
           K+VE+KD ILIMLDDLW EYDLEKE GIPCR SES KE CKILMT RSRDI TN+MNT++
Sbjct: 251 KLVEMKDKILIMLDDLWNEYDLEKEVGIPCR-SESSKEGCKILMTSRSRDILTNKMNTKK 310

Query: 312 LFEVNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGKKVQV 371
            F+VN LS++ESWKFF+AIVGEF +   + IA++VVK+CGGLPIALKI+AKALKGK++ +
Sbjct: 311 CFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKALKGKEIHI 370

Query: 372 WKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISVEDL 431
           WKDA +K + HVL+ I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I V+DL
Sbjct: 371 WKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHNICVKDL 430

Query: 432 QMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIA 491
           QMYAM MGLLNDINNWE  KNRVI LVDDLKSSYLLLESN  DNYV MHDVVRDVA YIA
Sbjct: 431 QMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIA 490

Query: 492 SKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQR 551
           S  +KM SL+YGCGQ+EW EEDR GSYNAIFV+C DF +LP   EFP LQLL+L   +  
Sbjct: 491 SNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFF 550

Query: 552 KE-KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
            E +AIQIP  FFEGME LRVLDMT MCFEPS+TS+THSL+NL+TL
Sbjct: 551 GEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTL 593

BLAST of Spg035314 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 1.2e-213
Identity = 390/590 (66.10%), Postives = 470/590 (79.66%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           IV  V +Y   PIGRQLGYLFFY +NIKD+EKQL++L+  RE+VQ LV E RSN+Y+IYD
Sbjct: 7   IVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAYEIYD 66

Query: 68  HVLTW-LDEVDVELLETLRQN------EASNMWCFNCIQWHRLARKTKKRLEYIHKLIDD 127
            V TW L EVDVEL     +N      + SN  C N +Q H+L+R++KKR++ I ++I+ 
Sbjct: 67  EVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILEIIEK 126

Query: 128 GNKFHQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGV 187
           G KF +  VG  APLP T   TLPADYQILESRTL+AE+IKD+LADPNVNKVGVCGMGGV
Sbjct: 127 GKKFDK--VGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMGGV 186

Query: 188 GKTKLLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAA 247
           GKTKLL EVKKLVLEN LF RVI V +G S GV+EIQD+IGG LNM LN  A +KE RA 
Sbjct: 187 GKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGRAP 246

Query: 248 LIQTKIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQM 307
            ++ K+VE+KD+ILIMLDDLW EYDL+KE GIPCR S S KE CKILMT RSRDI TN+M
Sbjct: 247 FLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCR-SRSSKEGCKILMTSRSRDILTNRM 306

Query: 308 NTERLFEVNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGK 367
           + E+ FEVN LS+EESWKFF+AIVGEF ++  + +AR+VVK+CGGLPIALKI+AKALKGK
Sbjct: 307 DAEKCFEVNSLSEEESWKFFIAIVGEFDKNRIEHLARNVVKKCGGLPIALKIIAKALKGK 366

Query: 368 KVQVWKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYIS 427
            V++WKDAL+K +  V + I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I 
Sbjct: 367 DVRIWKDALEKLEKRVSVKIKGVSDQLYSCLKLSYDRIEDEETRLLFLLCSVFPDDHKIH 426

Query: 428 VEDLQMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVA 487
           V+DLQMYAM MGLLNDI+NWE ++N V++LVD LKSS LLLESN  DNYV MHDVVRDVA
Sbjct: 427 VKDLQMYAMGMGLLNDIDNWETSRNMVVELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 486

Query: 488 IYIASKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRN 547
            YIA+K NKM SL+YG GQ+EWRE++R GSYN IF++C DF NLPP +EFP LQ+L+LR 
Sbjct: 487 KYIATKENKMSSLSYGRGQSEWREKERCGSYNVIFIDCVDFPNLPPNMEFPDLQMLILRI 546

Query: 548 ETQRKE-KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           ++   E +AIQIP+  FEGM+ LRVLDMT MCFEPSKTS THSL+NL+TL
Sbjct: 547 QSFFGEGRAIQIPEALFEGMKKLRVLDMTKMCFEPSKTSFTHSLKNLQTL 593

BLAST of Spg035314 vs. ExPASy TrEMBL
Match: A0A6J1DQJ3 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111022191 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.6e-162
Identity = 326/583 (55.92%), Postives = 392/583 (67.24%), Query Frame = 0

Query: 8   IVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYD 67
           +V  + +Y   PIGRQLGYLFFY RNIKDLEKQL++L+   E+VQ LV E+         
Sbjct: 7   VVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTGEKVQILVNEE--------- 66

Query: 68  HVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQY 127
                                                       E IH          + 
Sbjct: 67  --------------------------------------------EVIHM-----KSMTKS 126

Query: 128 NVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLG 187
             GC    P++                             NVNKVGV GMGGVGKTK L 
Sbjct: 127 RHGC---WPKS-----------------------------NVNKVGVYGMGGVGKTKWLD 186

Query: 188 EVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLN--ANSKEVRAALIQTKIV 247
           EVKKLVLEN LF RVI V +G S GV+EIQD+IGG LNM LN  A +KE RA  +Q K+V
Sbjct: 187 EVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLV 246

Query: 248 EIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFE 307
           E+++NILI+LDDLW EYDL+KE GIPCR SES KE CKILMT RSR I TN MNTE+ F+
Sbjct: 247 EMRENILILLDDLWNEYDLQKEVGIPCR-SESSKEGCKILMTSRSRHILTNNMNTEKCFK 306

Query: 308 VNPLSDEESWKFFVAIVGEFVQDGTKQIARDVVKECGGLPIALKILAKALKGKKVQVWKD 367
           VN LS+EESWKFF+ IVGEF ++  + +A++VVK+CGGLPIALKI+AKALKGK++++WK+
Sbjct: 307 VNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGKELRIWKN 366

Query: 368 ALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISVEDLQMY 427
           AL+K +  V + I GV+DQLY+CLK+SYD +E  E + LFLLC VFPDDH I  +DLQMY
Sbjct: 367 ALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMY 426

Query: 428 AMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKI 487
           AM MGLLN+++NWE  +N VI+LVD LKSS LLLESN  DNYV MHDVVRDVA YIAS  
Sbjct: 427 AMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNE 486

Query: 488 NKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKE- 547
           N M SL+YGCGQ+EWRE+DR GSYNAIFV+C DF NLP  LEFP LQ+L+LR +T   E 
Sbjct: 487 NNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEG 498

Query: 548 KAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           +AIQIPD FFEGM+ LRVLDMT M FE S+TSLT+SL+NL+TL
Sbjct: 547 QAIQIPDTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTL 498

BLAST of Spg035314 vs. ExPASy TrEMBL
Match: A0A6J1FY21 (probable disease resistance protein At4g27220 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448887 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 4.9e-151
Identity = 299/590 (50.68%), Postives = 399/590 (67.63%), Query Frame = 0

Query: 1   METLAAIIVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRS 60
           ME L ++    + +Y+  PIGRQL YL  Y RN   L++QL+ L+  R  VQ  V E RS
Sbjct: 1   MEILVSVAESIMGEYVIKPIGRQLSYLLCYNRNKNKLKEQLEALKTARRDVQGRVQEARS 60

Query: 61  NSYKIYDHVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDD 120
           N+Y I + V  WL + D  + + L  N +SN   FN +  ++L RKTKK++E +H+LI  
Sbjct: 61  NAYAIREEVSKWLTDADNAIHDELSSN-SSNPSAFNLVDRYQLGRKTKKKIEALHELI-- 120

Query: 121 GNKFHQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGV 180
             + +   VG  APL +T    +P  YQ+LES+  MA+QIK AL    V++VGV GMGGV
Sbjct: 121 AKRINFVEVGYPAPLLETKNIIVPGGYQVLESKISMAKQIKKALQKAEVSRVGVYGMGGV 180

Query: 181 GKTKLLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLNANSKEVRAALI 240
           GKT LL EVKK+VLE  +F RVI V VG S GVI+IQ++IG +LN++L   SKE RA+ I
Sbjct: 181 GKTYLLNEVKKMVLEEKVFDRVIEVCVGQSNGVIQIQEQIGDVLNVRL-PKSKEGRASFI 240

Query: 241 QTKIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNT 300
           +  +V+++ ++LI+LDDLWKEYDL KE GIPC         CK+LMT RS+DI  N MNT
Sbjct: 241 RNNLVKMEGSVLIILDDLWKEYDLVKEIGIPC-------SSCKVLMTSRSQDILMNNMNT 300

Query: 301 ERLFEVNPLSDEESWKFFVAIVG-EFVQDGTKQIARDVVKECGGLPIALKILAKALKGKK 360
              F+VN LS+EESWKFF  I+G EF     + +A+ VV+ECGGLP+AL  +AKALKGK 
Sbjct: 301 TMCFQVNSLSEEESWKFFTEIIGDEFDTPDMENLAKMVVRECGGLPLALDTVAKALKGKH 360

Query: 361 VQVWKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISV 420
           +  WKDAL K +N + M I GV+D++Y  L++SYD + G E K +FLLC VFPDD+ IS+
Sbjct: 361 MNHWKDALSKLRNSIGMDIKGVSDKVYASLRLSYDHLVGEEPKLMFLLCSVFPDDYKISL 420

Query: 421 EDLQMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQW--DNYVLMHDVVRDV 480
            DLQMYAM M +L  +N WED KNRV+KLV DLKS  LLL++++   D YV MHDVVRDV
Sbjct: 421 NDLQMYAMGMRILRKVNTWEDMKNRVMKLVHDLKSCSLLLDADEHSRDTYVKMHDVVRDV 480

Query: 481 AIYIASKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLR 540
           AI+IAS    M ++++G   ++W +E R G+Y AIFVNC++F  LP  L FP L+LL+LR
Sbjct: 481 AIHIASMEGIMTTMSFGSKLSDWEDEYRSGAYRAIFVNCDNFQKLPQNLNFPDLELLILR 540

Query: 541 NETQRKEKAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           +        ++IPDGFF GME ++ LD+T M F   +TS   SL+N+RTL
Sbjct: 541 SSNGFVGN-LEIPDGFFAGMEKIKALDITGMSF--LQTS-WRSLKNIRTL 575

BLAST of Spg035314 vs. ExPASy TrEMBL
Match: A0A6J1FYN5 (probable disease resistance protein At4g27220 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111448887 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 4.9e-151
Identity = 299/590 (50.68%), Postives = 399/590 (67.63%), Query Frame = 0

Query: 1   METLAAIIVEPVVKYITVPIGRQLGYLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRS 60
           ME L ++    + +Y+  PIGRQL YL  Y RN   L++QL+ L+  R  VQ  V E RS
Sbjct: 1   MEILVSVAESIMGEYVIKPIGRQLSYLLCYNRNKNKLKEQLEALKTARRDVQGRVQEARS 60

Query: 61  NSYKIYDHVLTWLDEVDVELLETLRQNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDD 120
           N+Y I + V  WL + D  + + L  N +SN   FN +  ++L RKTKK++E +H+LI  
Sbjct: 61  NAYAIREEVSKWLTDADNAIHDELSSN-SSNPSAFNLVDRYQLGRKTKKKIEALHELI-- 120

Query: 121 GNKFHQYNVGCSAPLPQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGV 180
             + +   VG  APL +T    +P  YQ+LES+  MA+QIK AL    V++VGV GMGGV
Sbjct: 121 AKRINFVEVGYPAPLLETKNIIVPGGYQVLESKISMAKQIKKALQKAEVSRVGVYGMGGV 180

Query: 181 GKTKLLGEVKKLVLENNLFHRVIVVIVGTSKGVIEIQDEIGGILNMKLNANSKEVRAALI 240
           GKT LL EVKK+VLE  +F RVI V VG S GVI+IQ++IG +LN++L   SKE RA+ I
Sbjct: 181 GKTYLLNEVKKMVLEEKVFDRVIEVCVGQSNGVIQIQEQIGDVLNVRL-PKSKEGRASFI 240

Query: 241 QTKIVEIKDNILIMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNT 300
           +  +V+++ ++LI+LDDLWKEYDL KE GIPC         CK+LMT RS+DI  N MNT
Sbjct: 241 RNNLVKMEGSVLIILDDLWKEYDLVKEIGIPC-------SSCKVLMTSRSQDILMNNMNT 300

Query: 301 ERLFEVNPLSDEESWKFFVAIVG-EFVQDGTKQIARDVVKECGGLPIALKILAKALKGKK 360
              F+VN LS+EESWKFF  I+G EF     + +A+ VV+ECGGLP+AL  +AKALKGK 
Sbjct: 301 TMCFQVNSLSEEESWKFFTEIIGDEFDTPDMENLAKMVVRECGGLPLALDTVAKALKGKH 360

Query: 361 VQVWKDALKKFKNHVLMGIPGVNDQLYTCLKISYDSVEG-EAKSLFLLCGVFPDDHYISV 420
           +  WKDAL K +N + M I GV+D++Y  L++SYD + G E K +FLLC VFPDD+ IS+
Sbjct: 361 MNHWKDALSKLRNSIGMDIKGVSDKVYASLRLSYDHLVGEEPKLMFLLCSVFPDDYKISL 420

Query: 421 EDLQMYAMSMGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQW--DNYVLMHDVVRDV 480
            DLQMYAM M +L  +N WED KNRV+KLV DLKS  LLL++++   D YV MHDVVRDV
Sbjct: 421 NDLQMYAMGMRILRKVNTWEDMKNRVMKLVHDLKSCSLLLDADEHSRDTYVKMHDVVRDV 480

Query: 481 AIYIASKINKMPSLTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLR 540
           AI+IAS    M ++++G   ++W +E R G+Y AIFVNC++F  LP  L FP L+LL+LR
Sbjct: 481 AIHIASMEGIMTTMSFGSKLSDWEDEYRSGAYRAIFVNCDNFQKLPQNLNFPDLELLILR 540

Query: 541 NETQRKEKAIQIPDGFFEGMENLRVLDMTNMCFEPSKTSLTHSLRNLRTL 587
           +        ++IPDGFF GME ++ LD+T M F   +TS   SL+N+RTL
Sbjct: 541 SSNGFVGN-LEIPDGFFAGMEKIKALDITGMSF--LQTS-WRSLKNIRTL 575

BLAST of Spg035314 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 214.9 bits (546), Expect = 1.7e-55
Identity = 175/564 (31.03%), Postives = 282/564 (50.00%), Query Frame = 0

Query: 35  KDLEKQLKD-LELIRERVQ--SLVTEQRSNSYKIYDHVLTWLDEVDVELLETLRQNEASN 94
           K LE++L+  L  + E V    L+ E+RS+          WL + DVE+LE +++ E   
Sbjct: 35  KSLERKLRIWLRKVEENVPLGELILEKRSS-------CAIWLSDKDVEILEKVKRLEEQG 94

Query: 95  MWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQTNCPTLPADYQILE 154
                 I  ++ +R+  +R+         G  FH          PQ             +
Sbjct: 95  QDLIKKISVNKSSREIVERVL--------GPSFH----------PQ-------------K 154

Query: 155 SRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLE---NNLFHRVIVVIVG 214
           +   M +++KD L   NV K+GV GMGGVGKT L+  +   +L+      F  VI V V 
Sbjct: 155 TALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVS 214

Query: 215 TSKGVIEIQDEIGGILNMKLNANSKEVRAALIQTKIVEIKDNILIMLDDLWKEYDLEKEF 274
               +  +Q +I   L  +            I  +++++K N L++LDD+W   DL+ + 
Sbjct: 215 KDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLK-NFLLILDDVWHPIDLD-QL 274

Query: 275 GIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWKFFVAIVGEFV-Q 334
           GIP   +  R +  K+++T R  ++   QM T    +V  L ++E+W+ F   VGE    
Sbjct: 275 GIPL--ALERSKDSKVVLTSRRLEV-CQQMMTNENIKVACLQEKEAWELFCHNVGEVANS 334

Query: 335 DGTKQIARDVVKECGGLPIALKILAKALKGK-KVQVWKDALKKFKNHVLMGIPGVN--DQ 394
           D  K IA+DV  EC GLP+A+  + + L+GK +V+VWK  L   K       P ++  ++
Sbjct: 335 DNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR----SAPSIDTEEK 394

Query: 395 LYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMYAMSMGLLNDINNWEDAKNRV 454
           ++  LK+SYD ++   KS FL C +FP+D+ I V +L MY ++ GLL+  +++ED  N  
Sbjct: 395 IFGTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEG 454

Query: 455 IKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAI-YIASKINKMPSLTY-GCGQNEWREE 514
           + LV+ LK S LL + +  D  V MHDVVRD AI +++S+     SL   G G  E+ ++
Sbjct: 455 VTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQD 514

Query: 515 DRRGSYNAIFVNCEDFLNLPPK-LEFPTLQLLVLRNETQRKEKAIQIPDGFFEGMENLRV 574
               S   + +       LP   +E     +L+L+  +  KE    +P+GF +   NLR+
Sbjct: 515 KFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKE----VPNGFLQAFPNLRI 546

Query: 575 LDMTNMCFE--PSKTSLTHSLRNL 584
           LD++ +     P   S  HSLR+L
Sbjct: 575 LDLSGVRIRTLPDSFSNLHSLRSL 546

BLAST of Spg035314 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 204.5 bits (519), Expect = 2.3e-52
Identity = 163/549 (29.69%), Postives = 277/549 (50.46%), Query Frame = 0

Query: 30  YQRNIKDLEKQLKDLELIR----ERVQSLVTEQRSNSYKIYDHVLTWLDEVDVELLETLR 89
           ++ N+K L + L+ L  ++    E  ++L+T+ +    K+    + W  E + E++   R
Sbjct: 29  FKSNVKALNESLERLTELKGNMSEDHETLLTKDKPLRLKL----MRWQREAE-EVISKAR 88

Query: 90  QNEASNMWCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQTNCPTLPA 149
                 + C   ++  R++RK  K L+ +  L  DG +F       S P    + P +  
Sbjct: 89  LKLEERVSCGMSLR-PRMSRKLVKILDEVKMLEKDGIEFVDMLSVESTPERVEHVPGVSV 148

Query: 150 DYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLENNL---FHRV 209
            +Q + S   M  +I+D L      K+GV GMGGVGKT L+  +   + E      F  V
Sbjct: 149 VHQTMASN--MLAKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLV 208

Query: 210 IVVIVGTSKGVIEIQDEIGGILNMKLNANSKEVRAALIQTKIVEIKDNILIMLDDLWKEY 269
           I VIV       E+Q +I   L++       E + A      +  +   L++LDD+WK  
Sbjct: 209 IFVIVSKEFDPREVQKQIAERLDIDTQMEESEEKLARRIYVGLMKERKFLLILDDVWKPI 268

Query: 270 DLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWKFFVAIV 329
           DL+   GIP R  E++    K+++T R  ++    M T+    V+ L +E++W+ F    
Sbjct: 269 DLDL-LGIP-RTEENKGS--KVILTSRFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNA 328

Query: 330 GEFVQ-DGTKQIARDVVKECGGLPIALKILAKALKGKK-VQVWKDALKKFKNHVLMGIPG 389
           G+ V+ D  ++IA+ V +ECGGLP+A+  +  A++GKK V++W   L K    V   I  
Sbjct: 329 GDVVRSDHVRKIAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSV-PWIKS 388

Query: 390 VNDQLYTCLKISYDSVEGEAKSLFLLCGVFPDDHYISVEDLQMYAMSMGLLNDINNWEDA 449
           + ++++  LK+SYD +E +AK  FLLC +FP+D+ I V ++  Y M+ G + ++ + ED+
Sbjct: 389 IEEKIFQPLKLSYDFLEDKAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDS 448

Query: 450 KNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYI--ASKINKMPSLTYGCGQNE 509
            N  I  V+ LK  Y LLE     + V MHDVVRD AI+I  +S+ +    +  G G  +
Sbjct: 449 MNEGITTVESLK-DYCLLEDGDRRDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQD 508

Query: 510 WREEDRRGSYNAIFVNCEDFLNLPPKLE--FPTLQLLVLRNETQRKEKAIQIPDGFFEGM 566
            R++    S   + +      +LP  +E       +L+L+     KE    +P GF +  
Sbjct: 509 IRQDKLAPSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGNFLLKE----VPIGFLQAF 558

BLAST of Spg035314 vs. TAIR 10
Match: AT1G61180.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 179.5 bits (454), Expect = 7.9e-45
Identity = 156/561 (27.81%), Postives = 269/561 (47.95%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLTWLD---EVDVE--- 85
           Y+   ++N++ L+++++DL  I+  VQ+ V    +   +  + V  WLD    VD+E   
Sbjct: 26  YIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEAVQVWLDRVNSVDIECKD 85

Query: 86  LLETLRQNEASNMWCFNCIQW----HRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPL 145
           LL            C  C ++    ++  +K    LE + KL  +GN F +     S P 
Sbjct: 86  LLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLNSEGN-FDE----VSQPP 145

Query: 146 PQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLE 205
           P++     P    I   +  M E+  + L +  V  +G+ GMGGVGKT L  ++     E
Sbjct: 146 PRSEVEERPTQPTI--GQEDMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAE 205

Query: 206 -NNLFHRVIVVIVGTSKGVIEIQDEIGGILNM--KLNANSKEVRAALIQTKIVEIKDNIL 265
               F  VI ++V     + ++Q++I   L++   L  N  E   A    ++++ K   +
Sbjct: 206 IGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGK-RFV 265

Query: 266 IMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDE 325
           +MLDD+W++ DLE   GIP     S   +CK+  T RSR++   +M   +  +VN L  E
Sbjct: 266 LMLDDIWEKVDLE-AIGIP---YPSEVNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPE 325

Query: 326 ESWKFFVAIVGEFVQDGTK---QIARDVVKECGGLPIALKILAKALKGK-KVQVWKDALK 385
           ++W+ F   VG+          ++AR+V ++C GLP+AL ++ + +  K  VQ W+ A+ 
Sbjct: 326 DAWELFKNKVGDNTLSSDPVIVELAREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIH 385

Query: 386 KFKNHVLMGIPGVNDQLYTCLKISYDSVEGE-AKSLFLLCGVFPDDHYISVEDLQMYAMS 445
            F N        + +++   LK SYDS+  E  KS FL C +FP+D  I  E L  Y + 
Sbjct: 386 VF-NTSAAEFSDMQNKILPILKYSYDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWIC 445

Query: 446 MGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKINKM 505
            G + +    + A+N+   ++  L  + LL +   +  Y +MHDVVR++A++IAS   K 
Sbjct: 446 EGFIGEDQVIKRARNKGYAMLGTLTRANLLTKVGTY--YCVMHDVVREMALWIASDFGKQ 505

Query: 506 PS---LTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEK 565
                +  G G +E  +    G+   + +   D   +  + +   L  L L++      K
Sbjct: 506 KENFVVQAGVGLHEIPKVKDWGAVRKMSLMDNDIEEITCESKCSELTTLFLQS-----NK 565

BLAST of Spg035314 vs. TAIR 10
Match: AT1G61180.2 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 179.5 bits (454), Expect = 7.9e-45
Identity = 156/561 (27.81%), Postives = 269/561 (47.95%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIRERVQSLVTEQRSNSYKIYDHVLTWLD---EVDVE--- 85
           Y+   ++N++ L+++++DL  I+  VQ+ V    +   +  + V  WLD    VD+E   
Sbjct: 26  YIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEAVQVWLDRVNSVDIECKD 85

Query: 86  LLETLRQNEASNMWCFNCIQW----HRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPL 145
           LL            C  C ++    ++  +K    LE + KL  +GN F +     S P 
Sbjct: 86  LLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLNSEGN-FDE----VSQPP 145

Query: 146 PQTNCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEVKKLVLE 205
           P++     P    I   +  M E+  + L +  V  +G+ GMGGVGKT L  ++     E
Sbjct: 146 PRSEVEERPTQPTI--GQEDMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAE 205

Query: 206 -NNLFHRVIVVIVGTSKGVIEIQDEIGGILNM--KLNANSKEVRAALIQTKIVEIKDNIL 265
               F  VI ++V     + ++Q++I   L++   L  N  E   A    ++++ K   +
Sbjct: 206 IGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGK-RFV 265

Query: 266 IMLDDLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDE 325
           +MLDD+W++ DLE   GIP     S   +CK+  T RSR++   +M   +  +VN L  E
Sbjct: 266 LMLDDIWEKVDLE-AIGIP---YPSEVNKCKVAFTTRSREV-CGEMGDHKPMQVNCLEPE 325

Query: 326 ESWKFFVAIVGEFVQDGTK---QIARDVVKECGGLPIALKILAKALKGK-KVQVWKDALK 385
           ++W+ F   VG+          ++AR+V ++C GLP+AL ++ + +  K  VQ W+ A+ 
Sbjct: 326 DAWELFKNKVGDNTLSSDPVIVELAREVAQKCRGLPLALNVIGETMSSKTMVQEWEHAIH 385

Query: 386 KFKNHVLMGIPGVNDQLYTCLKISYDSVEGE-AKSLFLLCGVFPDDHYISVEDLQMYAMS 445
            F N        + +++   LK SYDS+  E  KS FL C +FP+D  I  E L  Y + 
Sbjct: 386 VF-NTSAAEFSDMQNKILPILKYSYDSLGDEHIKSCFLYCALFPEDGEIYNEKLIDYWIC 445

Query: 446 MGLLNDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKINKM 505
            G + +    + A+N+   ++  L  + LL +   +  Y +MHDVVR++A++IAS   K 
Sbjct: 446 EGFIGEDQVIKRARNKGYAMLGTLTRANLLTKVGTY--YCVMHDVVREMALWIASDFGKQ 505

Query: 506 PS---LTYGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEK 565
                +  G G +E  +    G+   + +   D   +  + +   L  L L++      K
Sbjct: 506 KENFVVQAGVGLHEIPKVKDWGAVRKMSLMDNDIEEITCESKCSELTTLFLQS-----NK 565

BLAST of Spg035314 vs. TAIR 10
Match: AT1G12220.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 174.9 bits (442), Expect = 1.9e-43
Identity = 154/554 (27.80%), Postives = 267/554 (48.19%), Query Frame = 0

Query: 26  YLFFYQRNIKDLEKQLKDLELIR-ERVQSLVTEQRSNSYKIYDHVLTWLDEVDV---ELL 85
           Y+    +N+  L+K ++ L+  + + ++ L TE+ +   +    V  WL  V +   +  
Sbjct: 28  YIHNLSKNLASLQKAMRMLKARQYDVIRRLETEEFTGRQQRLSQVQVWLTSVLIIQNQFN 87

Query: 86  ETLRQNEASNM---WCFNCIQWHRLARKTKKRLEYIHKLIDDGNKFHQYNVGCSAPLPQT 145
           + LR NE        C  C +  +L+ +  KR+  + K ++  +    ++V  S   P  
Sbjct: 88  DLLRSNEVELQRLCLCGFCSKDLKLSYRYGKRVIMMLKEVESLSSQGFFDV-VSEATPFA 147

Query: 146 NCPTLPADYQILESRTLMAEQIKDALADPNVNKVGVCGMGGVGKTKLLGEV-KKLVLENN 205
           +   +P    I+  + +M E+  + L +     +G+ GMGGVGKT LL ++  K    ++
Sbjct: 148 DVDEIPFQPTIV-GQEIMLEKAWNRLMEDGSGILGLYGMGGVGKTTLLTKINNKFSKIDD 207

Query: 206 LFHRVIVVIVGTSKGVIEIQDEIGGILNM-KLNANSKEVRAALIQTKIVEIKDNILIMLD 265
            F  VI V+V  S  V +IQ +I   + +  +  + K      +    V  +   +++LD
Sbjct: 208 RFDVVIWVVVSRSSTVRKIQRDIAEKVGLGGMEWSEKNDNQIAVDIHNVLRRRKFVLLLD 267

Query: 266 DLWKEYDLEKEFGIPCRHSESRKERCKILMTGRSRDIFTNQMNTERLFEVNPLSDEESWK 325
           D+W++ +L K  G+P     S+   CK+  T RSRD+   +M  +   EV+ L  EESW 
Sbjct: 268 DIWEKVNL-KAVGVP---YPSKDNGCKVAFTTRSRDV-CGRMGVDDPMEVSCLQPEESWD 327

Query: 326 FFVAIVGEFVQDGTKQI---ARDVVKECGGLPIALKILAKALKGKK-VQVWKDALKKFKN 385
            F   VG+        I   AR V ++C GLP+AL ++ +A+  K+ V  W  A+    +
Sbjct: 328 LFQMKVGKNTLGSHPDIPGLARKVARKCRGLPLALNVIGEAMACKRTVHEWCHAIDVLTS 387

Query: 386 HVLMGIPGVNDQLYTCLKISYDSVEGE-AKSLFLLCGVFPDDHYISVEDLQMYAMSMGLL 445
             +    G+ D++   LK SYD++ GE  KS FL C +FP+D+ I  E L  Y +S G +
Sbjct: 388 SAI-DFSGMEDEILHVLKYSYDNLNGELMKSCFLYCSLFPEDYLIDKEGLVDYWISEGFI 447

Query: 446 NDINNWEDAKNRVIKLVDDLKSSYLLLESNQWDNYVLMHDVVRDVAIYIASKINKMPSLT 505
           N+    E   N+  +++  L  + LLLE  +  + V MHDVVR++A++I+S + K     
Sbjct: 448 NEKEGRERNINQGYEIIGTLVRACLLLEEERNKSNVKMHDVVREMALWISSDLGKQKEKC 507

Query: 506 YGCGQNEWREEDRRGSYNAIFVNCEDFLNLPPKLEFPTLQLLVLRNETQRKEKAIQIPDG 565
                   RE  +   +N   V     +N   +  F + +   L     +K   ++I   
Sbjct: 508 IVRAGVGLREVPKVKDWNT--VRKISLMNNEIEEIFDSHECAALTTLFLQKNDVVKISAE 567