Spg034994 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg034994
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionpolygalacturonase-like
Locationscaffold3: 25361284 .. 25363693 (-)
RNA-Seq ExpressionSpg034994
SyntenySpg034994
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCCATTTTCCCATCGCTTCCAATCAAACCACTTTGTAAAATTCAGTTTTCAAATGGCTCTTCTTGTTAGCTTACAGCTTCTTCTTTCCCTTGTGCTGCTGTTTTCGAACCATGCCTTGGCTGCGGGATTGAAGTTTGATATCGTTAGTCTCGGCGCCAAGCCCGACGGCGAGACCGACGCCTCGCATGCCTTGCAAGCTGCATGGGCCAGTGCCTGTGGCTCATCTGAGGCGGCCACCATTTACGTGCCCAAAGGCAAATTCTATGTTCAAAGTGGAGACTTCAATGGGCCTTGTAAGAACGATGCTATTACTGTGGTCATCGATGGCACTCTCGTGGCTTCGTCAGACATTCAAGTTCTTGCGAAATCGGAATCCTGGATTGCGTTCAGACACCTAAATGGGCTTTCCATTTCTGGCGGTGTTCTTGACGGCCAAGGAATTGGCGTATGGAACTGCAAACACTCGGGGAAGTCTTGCCCTGTTGGAGCCACGGTATGCAATTTTAAGTTGACTAGATTTTAAAGGGGTTGATTTTCTTTCAATTTTCTCGGCGGAAATTATAATAAAAATGGAAGATTTCTTTTTAAATTTGTTTATTGAAATTCTTTAAAGAAATTAGAATGTCCCTGCAAATCAAGAACGAGTGTATAAGAGCCCGTTTGATAACGTTCTCGCTTCCCGTTTCTTGTTTTTTAAGAATTAAAAACAAAAACATGTTTGATAGCTATTTTTGTTTTTTGTTTCTTTAAGAAAAAGAAATAGAAACAAAAAATGCGTTTGATAACTGTTTTTTGTTTATTGATTTTTAGTTAAATAAAAATTTAGTACACATGATATAAAAATATTTATAACATACATTAAAATAAATATAAAACTAGTATATTGAGTTTATCGTATTTATTCAGTTCCAACAAAAAATGAAAAAAAAAATAATTTTTATCCATCAAATATTGTTCAATTTGATTTGCATGTATTTTTTTACTTGAATAATAAAATTTACTTTTTTTTTTCCTTTAAAAATGCATGTCTTTCTTACTAGGGTATGATCCCAACACGTAAGAATAGAAAGAGAGAGAAAAAAATATATATTCTTTTGACAATTAATGACAAACGTGAAATATTTATTAATTAATTGATTTAATAAATAAAATTAAAAACCTACTAAAATTAATGAGAAATAAGAGTTTTAAGTAGAAAATAATAACTAAAAGCACATTAATGAAAAATGGATAAAAAGATTAAAAAAATAAATAACAGAAAAAAGAAACAAAAAGATTAAAAATGAAATAGAAAGAAATAACAGAAAAAAGAAAAAAAATTAAAAAAGAAAAGAGAAAAAAATGTAAAAAAAACAAGTAATAAGAAAAAATAGAGAGAGGAAGAGAAAAGATGCGTTTAGGAGAGGGAAAGAGAGAGAGGGTACATGCATGAAAAATGGGAAAAGAAAAATAGGAACAAAATTAGTTTCTCAAATTTTGCCTCTTTTATAGAAACGTTTCTAGAAATTAAGAAACGAGAAACAAGATGGGTTATCAAATATGTATGTTTCTCAAAAAATGAGAAACGGAAACAAAAAAACGGGAAACGAGAACATTATCAAATCAACCCTTAAGTATATACTCCAGTTAGGTTATGGCATGTGTATGCATATGTATGTGTATATATATATATATGAAGCAACAATATTAATTTAAATTAATACATGCTCTTGATTGCGTTGTACTAATTGTTGCTCTCAATCCTTTTGAATTTGCAGAATTTAGAAGTCTTAGATTCACAAAACGTAATGATCAGCGGATTATCATCAATCAATAGCCAAATGTTTAACATTGTGGTGAATGGTTGTCAAAATGTGAAAATGCAAGGAGTGAAGGTCTCCGCCGCTGGCGATAGTCCAAACACCGATGGCATCCATGTGCAGAAATCATCCAATGTCACCATCCTCAGCTCAAGCATTGGCACTGGCGATGACTGCATCTCAATCGGCCCGGGGACCTCCAACTTATGGATGGAAAGTATTGCATGTGGACCTGGACATGGAATCAGGTAAGGATAGATTATGAAGTAGCTACATACATATTGTAATAAGAAACATGTACTCATAAAAGTATTCGTTTGTTGTAGCATTGGGAGCTTAGGGAAGGGGTTGGAAGAGGCTGGAGTGGAAAACGTCACAGTGAAAACAGCTACTTTCACGGGGACTCAAAATGGAGTGAGGATTAAGTCATGGGGGAGGCCAAGCAATGGGTTCGTTAGAAATGTTATCTTTCAACACATTGTTTTCAACAATGTGAATAATCCTATCATTATTGATCAAGATTACTGTCCAGATGACAAAGGTTGCCCTGGCCAGGTGCATTTCCTCATTAATCGTCCCACTGAAGAAATAATCTTTGAGAAATTATAG

mRNA sequence

CTTCCATTTTCCCATCGCTTCCAATCAAACCACTTTGTAAAATTCAGTTTTCAAATGGCTCTTCTTGTTAGCTTACAGCTTCTTCTTTCCCTTGTGCTGCTGTTTTCGAACCATGCCTTGGCTGCGGGATTGAAGTTTGATATCGTTAGTCTCGGCGCCAAGCCCGACGGCGAGACCGACGCCTCGCATGCCTTGCAAGCTGCATGGGCCAGTGCCTGTGGCTCATCTGAGGCGGCCACCATTTACGTGCCCAAAGGCAAATTCTATGTTCAAAGTGGAGACTTCAATGGGCCTTGTAAGAACGATGCTATTACTGTGGTCATCGATGGCACTCTCGTGGCTTCGTCAGACATTCAAGTTCTTGCGAAATCGGAATCCTGGATTGCGTTCAGACACCTAAATGGGCTTTCCATTTCTGGCGGTGTTCTTGACGGCCAAGGAATTGGCGTATGGAACTGCAAACACTCGGGGAAGTCTTGCCCTGTTGGAGCCACGAATTTAGAAGTCTTAGATTCACAAAACGTAATGATCAGCGGATTATCATCAATCAATAGCCAAATGTTTAACATTGTGGTGAATGGTTGTCAAAATGTGAAAATGCAAGGAGTGAAGGTCTCCGCCGCTGGCGATAGTCCAAACACCGATGGCATCCATGTGCAGAAATCATCCAATGTCACCATCCTCAGCTCAAGCATTGGCACTGGCGATGACTGCATCTCAATCGGCCCGGGGACCTCCAACTTATGGATGGAAAGTATTGCATGTGGACCTGGACATGGAATCAGCATTGGGAGCTTAGGGAAGGGGTTGGAAGAGGCTGGAGTGGAAAACGTCACAGTGAAAACAGCTACTTTCACGGGGACTCAAAATGGAGTGAGGATTAAGTCATGGGGGAGGCCAAGCAATGGGTTCGTTAGAAATGTTATCTTTCAACACATTGTTTTCAACAATGTGAATAATCCTATCATTATTGATCAAGATTACTGTCCAGATGACAAAGGTTGCCCTGGCCAGGTGCATTTCCTCATTAATCGTCCCACTGAAGAAATAATCTTTGAGAAATTATAG

Coding sequence (CDS)

CTTCCATTTTCCCATCGCTTCCAATCAAACCACTTTGTAAAATTCAGTTTTCAAATGGCTCTTCTTGTTAGCTTACAGCTTCTTCTTTCCCTTGTGCTGCTGTTTTCGAACCATGCCTTGGCTGCGGGATTGAAGTTTGATATCGTTAGTCTCGGCGCCAAGCCCGACGGCGAGACCGACGCCTCGCATGCCTTGCAAGCTGCATGGGCCAGTGCCTGTGGCTCATCTGAGGCGGCCACCATTTACGTGCCCAAAGGCAAATTCTATGTTCAAAGTGGAGACTTCAATGGGCCTTGTAAGAACGATGCTATTACTGTGGTCATCGATGGCACTCTCGTGGCTTCGTCAGACATTCAAGTTCTTGCGAAATCGGAATCCTGGATTGCGTTCAGACACCTAAATGGGCTTTCCATTTCTGGCGGTGTTCTTGACGGCCAAGGAATTGGCGTATGGAACTGCAAACACTCGGGGAAGTCTTGCCCTGTTGGAGCCACGAATTTAGAAGTCTTAGATTCACAAAACGTAATGATCAGCGGATTATCATCAATCAATAGCCAAATGTTTAACATTGTGGTGAATGGTTGTCAAAATGTGAAAATGCAAGGAGTGAAGGTCTCCGCCGCTGGCGATAGTCCAAACACCGATGGCATCCATGTGCAGAAATCATCCAATGTCACCATCCTCAGCTCAAGCATTGGCACTGGCGATGACTGCATCTCAATCGGCCCGGGGACCTCCAACTTATGGATGGAAAGTATTGCATGTGGACCTGGACATGGAATCAGCATTGGGAGCTTAGGGAAGGGGTTGGAAGAGGCTGGAGTGGAAAACGTCACAGTGAAAACAGCTACTTTCACGGGGACTCAAAATGGAGTGAGGATTAAGTCATGGGGGAGGCCAAGCAATGGGTTCGTTAGAAATGTTATCTTTCAACACATTGTTTTCAACAATGTGAATAATCCTATCATTATTGATCAAGATTACTGTCCAGATGACAAAGGTTGCCCTGGCCAGGTGCATTTCCTCATTAATCGTCCCACTGAAGAAATAATCTTTGAGAAATTATAG

Protein sequence

LPFSHRFQSNHFVKFSFQMALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQDYCPDDKGCPGQVHFLINRPTEEIIFEKL
Homology
BLAST of Spg034994 vs. NCBI nr
Match: XP_038887074.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 5.9e-138
Identity = 243/320 (75.94%), Postives = 284/320 (88.75%), Query Frame = 0

Query: 19  MALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEA 78
           M +L +LQLLLSL+LLF  H LA GL FDIVSLGAKP+G+TD+S A Q AW +AC SS  
Sbjct: 1   MPVLGTLQLLLSLLLLFFTHDLATGLTFDIVSLGAKPNGKTDSSPAFQTAWTNACSSSVP 60

Query: 79  ATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSI 138
           ATIYVP G FYVQSG+FNGPCKN+AIT++IDGTL+ASSDIQVLA++ESWI+F+ +NGLSI
Sbjct: 61  ATIYVPNGVFYVQSGNFNGPCKNNAITILIDGTLMASSDIQVLAQTESWISFKDINGLSI 120

Query: 139 SGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 198
           SGGVLDGQGIG+WNCKHSG+SCP GATNL + ++QNV +SGLSS+NSQMFNIVV+GCQNV
Sbjct: 121 SGGVLDGQGIGLWNCKHSGQSCPDGATNLLISNAQNVKVSGLSSVNSQMFNIVVHGCQNV 180

Query: 199 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 258
            +QG+ VS AGDSPNTDGIHV++S NVTIL+SSIGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 LVQGMNVSDAGDSPNTDGIHVEQSLNVTILNSSIGTGDDCISIGPGTTNLWMENITCGPG 240

Query: 259 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 318
           HG+SIGSLGK  EEAGVENVTV TATF GT+NGVRIKSWGRPS+GF  NV+FQHIVF +V
Sbjct: 241 HGVSIGSLGKEAEEAGVENVTVSTATFRGTENGVRIKSWGRPSDGFATNVLFQHIVFYDV 300

Query: 319 NNPIIIDQDYCPDDKGCPGQ 339
            NP+IIDQ+YCP+ + CPGQ
Sbjct: 301 KNPLIIDQNYCPNHESCPGQ 320

BLAST of Spg034994 vs. NCBI nr
Match: XP_004140371.1 (polygalacturonase [Cucumis sativus])

HSP 1 Score: 501.5 bits (1290), Expect = 5.9e-138
Identity = 240/311 (77.17%), Postives = 279/311 (89.71%), Query Frame = 0

Query: 28  LLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 87
           LLSL+ LF NH LAAG  F+IVSLGA  DG+TDASHA Q AWA+ACGSSE ATIYVP G 
Sbjct: 9   LLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGA 68

Query: 88  FYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQG 147
           FY+QSG+F+GPCKN+AIT+VI+GTL+ASS+IQVLA+S++WIAFR +NGLSI GGV+DGQG
Sbjct: 69  FYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQG 128

Query: 148 IGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSA 207
           IG+WNCKHSGKSCP GATNLE+  +QNV ++GLSSINSQMFNIVV GC+NV++QGV VS+
Sbjct: 129 IGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSS 188

Query: 208 AGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLG 267
           AGDSPNTDGIHVQ+S NV I S+SIGTGDDCISIGPGT+NLWME+I CGPGHGISIGSLG
Sbjct: 189 AGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLG 248

Query: 268 KGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQD 327
           + +EEAGVENVTV TATFTGTQNGVRIKSWGRPSN F   ++FQHI F NVNNPI+IDQ+
Sbjct: 249 RQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNVNNPILIDQN 308

Query: 328 YCPDDKGCPGQ 339
           YCP+++GCPGQ
Sbjct: 309 YCPNNQGCPGQ 319

BLAST of Spg034994 vs. NCBI nr
Match: XP_008460550.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 484.2 bits (1245), Expect = 9.7e-133
Identity = 236/320 (73.75%), Postives = 277/320 (86.56%), Query Frame = 0

Query: 19  MALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEA 78
           M L+ +L LL  L+ LF NH LAAG  F+IVSLGAK DG TDAS A Q AWA+ACGSSE 
Sbjct: 1   MPLVGNLHLLF-LLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 79  ATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSI 138
           ATIYVP G FYV SG+F GPCKN+AIT++I+GTL+ASS+IQVLA+S++WIAFRH+NGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 139 SGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 198
            GGV+DGQG+G+WNCKHSG+SCP GAT+LE+ DSQNV ++GLSS+NSQMFNIVV GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 199 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 258
           ++QGV VS+A DSPNTDGIHVQ+S NV I +S+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 259 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 318
           HGISIGSLGK  EEAGV+NVTV TATF GTQNGVRIKSWGRPS+GF   ++FQHI+F NV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 319 NNPIIIDQDYCPDDKGCPGQ 339
           +NPI IDQ+YCP  + CPGQ
Sbjct: 301 SNPIFIDQNYCPHQQSCPGQ 319

BLAST of Spg034994 vs. NCBI nr
Match: KAA0062404.1 (polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-132
Identity = 236/320 (73.75%), Postives = 277/320 (86.56%), Query Frame = 0

Query: 19  MALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEA 78
           M L+ +L LL  L+ LF NH LAAG  F+IVSLGAK DG TDAS A Q AWA+ACGSSE 
Sbjct: 1   MPLVGNLHLLF-LLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 79  ATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSI 138
           ATIYVP G FYV SG+F GPCKN+AITV+I+GTL+ASS+IQVLA+S++WIAFRH+NGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 139 SGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 198
            GGV+DGQG+G+WNCKHSG+SCP GAT+LE+ DSQNV ++GLSS+NSQMFNIVV GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 199 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 258
           ++QGV VS+A DSPNTDGIHVQ+S NV I +S+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 259 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 318
           HGISIGSLGK  EEAGV+NVTV TATF GTQNGVRIKSWGRPS+GF   ++FQHI+F NV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 319 NNPIIIDQDYCPDDKGCPGQ 339
           +NPI IDQ+YCP  + CPG+
Sbjct: 301 SNPIFIDQNYCPHQQSCPGR 319

BLAST of Spg034994 vs. NCBI nr
Match: XP_022149030.1 (polygalacturonase-like [Momordica charantia])

HSP 1 Score: 481.9 bits (1239), Expect = 4.8e-132
Identity = 247/322 (76.71%), Postives = 272/322 (84.47%), Query Frame = 0

Query: 19  MALLVSLQLL-LSLVLLFSNHALA-AGLKFDIVSLGAKPDGETDASHALQAAWASACGSS 78
           MALL   QL+ LSL+LL  + AL   GL F+IVSLGAK DG TDAS ALQ AW  ACGS 
Sbjct: 1   MALLGCSQLVRLSLLLLLLDLALGHPGLTFNIVSLGAKADGRTDASFALQTAWGHACGSP 60

Query: 79  EAATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGL 138
             ATIYVP G FYVQSG F+GPCKN+AITV IDGTLVASSDI+VLA + SW+AFRHLNGL
Sbjct: 61  VPATIYVPNGTFYVQSGTFSGPCKNNAITVRIDGTLVASSDIEVLANTPSWMAFRHLNGL 120

Query: 139 SISGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQ 198
           SI GGVLDGQG G+W CK S   CPVGAT LEV D+QNVMISGLSS+NSQMF+IVV GC+
Sbjct: 121 SIYGGVLDGQGKGLWACKKSRSGCPVGATTLEVSDTQNVMISGLSSLNSQMFHIVVRGCE 180

Query: 199 NVKMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACG 258
           NVK+QG KV A GDSPNTDGIHVQ+SSNV ILSS+IGTGDDCISIGPGTS+LWME IACG
Sbjct: 181 NVKIQGAKVLAPGDSPNTDGIHVQQSSNVAILSSTIGTGDDCISIGPGTSHLWMEKIACG 240

Query: 259 PGHGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFN 318
           PGHGISIGSLGKG  EAGVENVTVKTA+FTGT NGVRIKSWGRPS+GF RNV F+HIVF+
Sbjct: 241 PGHGISIGSLGKGAVEAGVENVTVKTASFTGTMNGVRIKSWGRPSSGFARNVHFEHIVFD 300

Query: 319 NVNNPIIIDQDYCPDDKGCPGQ 339
           NV NP+I+DQ+YCP   GCPGQ
Sbjct: 301 NVENPVIVDQNYCPGGHGCPGQ 322

BLAST of Spg034994 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.1e-115
Identity = 201/313 (64.22%), Postives = 249/313 (79.55%), Query Frame = 0

Query: 29  LSLVLLFS-NHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 88
           LSL+ +F  N A+A+ L +++ SLGAK DG+TD++ A  +AWA AC S     IYVP G 
Sbjct: 10  LSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGT 69

Query: 89  FYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQG 148
           F+++   F+GPCKN+AIT  I GTLVA SD +V+  + +WI F H+NG++ISGG+LDGQG
Sbjct: 70  FFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQG 129

Query: 149 IGVWNCKH-SGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVS 208
             +W CK   G+SCP GAT L   DS N+++SGL+S+NSQMF+IV+N  QNV+MQGV+VS
Sbjct: 130 TALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVS 189

Query: 209 AAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSL 268
            +G+SPNTDGIHVQ SS VTIL+S I TGDDC+SIGPGTSNLW+E +ACGPGHGISIGSL
Sbjct: 190 RSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSL 249

Query: 269 GKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQ 328
           GK  EEAGV+NVTVKT TF+GTQNG+RIKSWGRPS GF RN++FQH    NV NPI+IDQ
Sbjct: 250 GKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQ 309

Query: 329 DYCPDDKGCPGQV 340
            YCPD+KGCPGQV
Sbjct: 310 HYCPDNKGCPGQV 322

BLAST of Spg034994 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.7e-85
Identity = 166/320 (51.88%), Postives = 219/320 (68.44%), Query Frame = 0

Query: 21  LLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAAT 80
           L++ L L++    L S+ A       +++S GAKPDG  D++ A  AAW  AC S+   T
Sbjct: 8   LILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTT 67

Query: 81  IYVPKGKFYVQSGDFNG-PCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSIS 140
           I VPKG+F V +  F+G  CK   I++ I G++VA  D +++A S+ WI F  +  +SI 
Sbjct: 68  IIVPKGRFLVGNLVFHGNECKQAPISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIY 127

Query: 141 GGVLDGQGIGVWNCKHS-GKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 200
           GG+LD QG  +W CK++ G +CP GA +L    S N+ ISGL+SINSQ F+IV++   NV
Sbjct: 128 GGILDAQGTSLWKCKNNGGHNCPTGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNV 187

Query: 201 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 260
            + GVKVSA  +SPNTDGIHV+ S +V I +S IGTGDDCISIGPG++N+++++I CGPG
Sbjct: 188 NIDGVKVSADENSPNTDGIHVESSHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPG 247

Query: 261 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 320
           HGISIGSLG+  EE GV+NVTV    F GT NGVRIK+WG+ SN F RN++FQHI    V
Sbjct: 248 HGISIGSLGRAEEEQGVDNVTVSNVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMV 307

Query: 321 NNPIIIDQDYCPDDKGCPGQ 339
            NPIIIDQ YC   K CP Q
Sbjct: 308 KNPIIIDQHYCL-HKPCPKQ 326

BLAST of Spg034994 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.0e-63
Identity = 130/294 (44.22%), Postives = 180/294 (61.22%), Query Frame = 0

Query: 41  AAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGKFYVQSGDFNGPCK 100
           ++G  F++   GAK  G  D S A+  AW +AC S   +T+ +PKG + +      GPCK
Sbjct: 5   SSGSVFNVNDYGAK--GAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCK 64

Query: 101 NDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISG-GVLDGQGIGVW---NCKHS 160
              I   IDG + A +D     KS+ W++F  ++GL++SG G LDGQG   W   NC   
Sbjct: 65  GSKIGFQIDGVVKAPADPSKF-KSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNC-DK 124

Query: 161 GKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSAAGDSPNTDG 220
             +C   A NL     ++ M+  ++S+NS+MF+I V  C+++  Q V V+A G S NTDG
Sbjct: 125 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 184

Query: 221 IHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLGKGLEEAGVE 280
           IHV  S  VTI ++ I TGDDCISIGPG+ N+ +  + CGPGHGISIGSLG+   E  V 
Sbjct: 185 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 244

Query: 281 NVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQDYCP 331
            +TVK  TF+GT NGVR+K+W     G   ++ FQ +  NNV NP+I+DQ+YCP
Sbjct: 245 GITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCP 294

BLAST of Spg034994 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 3.0e-60
Identity = 128/297 (43.10%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 39  ALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGKFYVQSGDFNGP 98
           A   G  FDI  LGA  +G+TD++ A+Q AWASACG +   TI +PKG F V   +F GP
Sbjct: 34  ASGPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGP 93

Query: 99  CKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISG-GVLDGQGIGVWNCKHSG 158
           CK D +T+ +DG L+A++D+    +  +WI    ++ L I+G G LDGQG  VW+     
Sbjct: 94  CKGD-VTIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCT 153

Query: 159 K--SCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSAAGDSPNTD 218
           K   C +   +L +    N  +SG++ +NS+ F++ +  C+N+ ++ V V+A GDSPNTD
Sbjct: 154 KKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTD 213

Query: 219 GIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLGKGLEEAGV 278
           GIH+  SS +TI ++ IG GDDCISIGPGTS + +  + CGPGHGISIGSLG+  +E  V
Sbjct: 214 GIHMGDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDV 273

Query: 279 ENVTVKTATFTGTQNGVRIKSWGRPSNGF-VRNVIFQHIVFNNVNNPIIIDQDYCPD 332
            ++ VK  T   T  GVRIK++   ++   V  + +++I   +  NPI ID  YCP+
Sbjct: 274 TDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPN 329

BLAST of Spg034994 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 128/297 (43.10%), Postives = 186/297 (62.63%), Query Frame = 0

Query: 39  ALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGKFYVQSGDFNGP 98
           A   G  FDI  LGA  +G+TD++ A+Q AWASACG +   TI +PKG F V   +F GP
Sbjct: 34  ASGPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGP 93

Query: 99  CKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISG-GVLDGQGIGVWNCKHSG 158
           CK D +T+ ++G L+A++D+       +WI    ++ L I+G G LDGQG  VW+     
Sbjct: 94  CKGD-VTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCV 153

Query: 159 K--SCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSAAGDSPNTD 218
           K   C +   +L +    N  +SG++ +NS+ F++ +  C+++ ++ V V+A GDSPNTD
Sbjct: 154 KKYDCKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTD 213

Query: 219 GIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLGKGLEEAGV 278
           GIH+  SS VTI ++ IG GDDCISIGPGTS + +  + CGPGHGISIGSLG+  +E  V
Sbjct: 214 GIHMGDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDV 273

Query: 279 ENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVI-FQHIVFNNVNNPIIIDQDYCPD 332
            ++ VK  T   T NGVRIK++   ++    + I +++I   +   PIIID  YCP+
Sbjct: 274 TDINVKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPN 329

BLAST of Spg034994 vs. ExPASy TrEMBL
Match: A0A0A0KN91 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.9e-138
Identity = 240/311 (77.17%), Postives = 279/311 (89.71%), Query Frame = 0

Query: 28  LLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 87
           LLSL+ LF NH LAAG  F+IVSLGA  DG+TDASHA Q AWA+ACGSSE ATIYVP G 
Sbjct: 9   LLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGA 68

Query: 88  FYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQG 147
           FY+QSG+F+GPCKN+AIT+VI+GTL+ASS+IQVLA+S++WIAFR +NGLSI GGV+DGQG
Sbjct: 69  FYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQG 128

Query: 148 IGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSA 207
           IG+WNCKHSGKSCP GATNLE+  +QNV ++GLSSINSQMFNIVV GC+NV++QGV VS+
Sbjct: 129 IGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSS 188

Query: 208 AGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLG 267
           AGDSPNTDGIHVQ+S NV I S+SIGTGDDCISIGPGT+NLWME+I CGPGHGISIGSLG
Sbjct: 189 AGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLG 248

Query: 268 KGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQD 327
           + +EEAGVENVTV TATFTGTQNGVRIKSWGRPSN F   ++FQHI F NVNNPI+IDQ+
Sbjct: 249 RQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNVNNPILIDQN 308

Query: 328 YCPDDKGCPGQ 339
           YCP+++GCPGQ
Sbjct: 309 YCPNNQGCPGQ 319

BLAST of Spg034994 vs. ExPASy TrEMBL
Match: A0A1S3CCA2 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.7e-133
Identity = 236/320 (73.75%), Postives = 277/320 (86.56%), Query Frame = 0

Query: 19  MALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEA 78
           M L+ +L LL  L+ LF NH LAAG  F+IVSLGAK DG TDAS A Q AWA+ACGSSE 
Sbjct: 1   MPLVGNLHLLF-LLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 79  ATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSI 138
           ATIYVP G FYV SG+F GPCKN+AIT++I+GTL+ASS+IQVLA+S++WIAFRH+NGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 139 SGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 198
            GGV+DGQG+G+WNCKHSG+SCP GAT+LE+ DSQNV ++GLSS+NSQMFNIVV GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 199 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 258
           ++QGV VS+A DSPNTDGIHVQ+S NV I +S+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 259 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 318
           HGISIGSLGK  EEAGV+NVTV TATF GTQNGVRIKSWGRPS+GF   ++FQHI+F NV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 319 NNPIIIDQDYCPDDKGCPGQ 339
           +NPI IDQ+YCP  + CPGQ
Sbjct: 301 SNPIFIDQNYCPHQQSCPGQ 319

BLAST of Spg034994 vs. ExPASy TrEMBL
Match: A0A5D3DS41 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001940 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.1e-132
Identity = 236/320 (73.75%), Postives = 277/320 (86.56%), Query Frame = 0

Query: 19  MALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEA 78
           M L+ +L LL  L+ LF NH LAAG  F+IVSLGAK DG TDAS A Q AWA+ACGSSE 
Sbjct: 1   MPLVGNLHLLF-LLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEP 60

Query: 79  ATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSI 138
           ATIYVP G FYV SG+F GPCKN+AITV+I+GTL+ASS+IQVLA+S++WIAFRH+NGLSI
Sbjct: 61  ATIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSI 120

Query: 139 SGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNV 198
            GGV+DGQG+G+WNCKHSG+SCP GAT+LE+ DSQNV ++GLSS+NSQMFNIVV GCQNV
Sbjct: 121 YGGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNV 180

Query: 199 KMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPG 258
           ++QGV VS+A DSPNTDGIHVQ+S NV I +S+IGTGDDCISIGPGT+NLWME+I CGPG
Sbjct: 181 QIQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPG 240

Query: 259 HGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNV 318
           HGISIGSLGK  EEAGV+NVTV TATF GTQNGVRIKSWGRPS+GF   ++FQHI+F NV
Sbjct: 241 HGISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNV 300

Query: 319 NNPIIIDQDYCPDDKGCPGQ 339
           +NPI IDQ+YCP  + CPG+
Sbjct: 301 SNPIFIDQNYCPHQQSCPGR 319

BLAST of Spg034994 vs. ExPASy TrEMBL
Match: A0A6J1D4L9 (polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111017549 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.3e-132
Identity = 247/322 (76.71%), Postives = 272/322 (84.47%), Query Frame = 0

Query: 19  MALLVSLQLL-LSLVLLFSNHALA-AGLKFDIVSLGAKPDGETDASHALQAAWASACGSS 78
           MALL   QL+ LSL+LL  + AL   GL F+IVSLGAK DG TDAS ALQ AW  ACGS 
Sbjct: 1   MALLGCSQLVRLSLLLLLLDLALGHPGLTFNIVSLGAKADGRTDASFALQTAWGHACGSP 60

Query: 79  EAATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGL 138
             ATIYVP G FYVQSG F+GPCKN+AITV IDGTLVASSDI+VLA + SW+AFRHLNGL
Sbjct: 61  VPATIYVPNGTFYVQSGTFSGPCKNNAITVRIDGTLVASSDIEVLANTPSWMAFRHLNGL 120

Query: 139 SISGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQ 198
           SI GGVLDGQG G+W CK S   CPVGAT LEV D+QNVMISGLSS+NSQMF+IVV GC+
Sbjct: 121 SIYGGVLDGQGKGLWACKKSRSGCPVGATTLEVSDTQNVMISGLSSLNSQMFHIVVRGCE 180

Query: 199 NVKMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACG 258
           NVK+QG KV A GDSPNTDGIHVQ+SSNV ILSS+IGTGDDCISIGPGTS+LWME IACG
Sbjct: 181 NVKIQGAKVLAPGDSPNTDGIHVQQSSNVAILSSTIGTGDDCISIGPGTSHLWMEKIACG 240

Query: 259 PGHGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFN 318
           PGHGISIGSLGKG  EAGVENVTVKTA+FTGT NGVRIKSWGRPS+GF RNV F+HIVF+
Sbjct: 241 PGHGISIGSLGKGAVEAGVENVTVKTASFTGTMNGVRIKSWGRPSSGFARNVHFEHIVFD 300

Query: 319 NVNNPIIIDQDYCPDDKGCPGQ 339
           NV NP+I+DQ+YCP   GCPGQ
Sbjct: 301 NVENPVIVDQNYCPGGHGCPGQ 322

BLAST of Spg034994 vs. ExPASy TrEMBL
Match: A0A6J1F489 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440267 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 4.0e-132
Identity = 239/336 (71.13%), Postives = 280/336 (83.33%), Query Frame = 0

Query: 9   SNHFVKFSFQMALLVSLQLLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAA 68
           +N  +KFS  ++LLV L + LSL+L + NHA AA L F+I++LGAKPD +TDAS  LQ A
Sbjct: 2   NNCIIKFS--LSLLVILHIFLSLLLWYFNHASAAELTFNIITLGAKPDEKTDASPVLQTA 61

Query: 69  WASACGSSEAATIYVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWI 128
           WA AC SSE ATIYVP+G F+V+S  FNGPCKN+AITV+IDGTLVASSDI VLA S SWI
Sbjct: 62  WAKACDSSEPATIYVPEGIFFVRSVRFNGPCKNNAITVLIDGTLVASSDIHVLANSSSWI 121

Query: 129 AFRHLNGLSISGGVLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMF 188
            F H+NGLSI+GG++DGQG  +WNCKHSGKSCP+GA +L++ D +NV I+GLSSINSQ+F
Sbjct: 122 LFNHINGLSITGGIIDGQGTPLWNCKHSGKSCPIGARSLQINDGKNVNINGLSSINSQLF 181

Query: 189 NIVVNGCQNVKMQGVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNL 248
            I +N CQNV M  V V AAGDSPNTDGIHVQ+SS+VTIL+S+IG GDDCISIGPGTSN+
Sbjct: 182 GIGMNRCQNVHMTRVNVLAAGDSPNTDGIHVQQSSDVTILNSNIGNGDDCISIGPGTSNM 241

Query: 249 WMESIACGPGHGISIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNV 308
           W+E+I CGPGHGISIGSLG G EE GV NVTVK++TFTGTQNGVRIKSWGRPSNGF  NV
Sbjct: 242 WIETITCGPGHGISIGSLGIGAEEDGVHNVTVKSSTFTGTQNGVRIKSWGRPSNGFATNV 301

Query: 309 IFQHIVFNNVNNPIIIDQDYCPDDKGCPGQVHFLIN 345
            FQHI  +NV NPIIIDQ+YCP  K CPGQV+FLIN
Sbjct: 302 HFQHITMDNVKNPIIIDQNYCPHLKDCPGQVYFLIN 335

BLAST of Spg034994 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.5e-110
Identity = 188/313 (60.06%), Postives = 243/313 (77.64%), Query Frame = 0

Query: 28  LLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 87
           LL ++L   + + ++   ++I+S GAKPDG+TD++ A    WA AC S +  TI VPKG+
Sbjct: 5   LLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGR 64

Query: 88  FYVQSGDFNG-PCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQ 147
           F ++S  F+G  CK  ++T  I GTLVA SD +V+ K   WI F+HL+G+S+ GGVLD Q
Sbjct: 65  FLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQ 124

Query: 148 GIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVS 207
           G  +W+CK SGK+CP GAT++    S+NV+ISGL+S+NSQMF++ +NGC NVK+ GVKVS
Sbjct: 125 GASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVS 184

Query: 208 AAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSL 267
           A G+SPNTDGIHVQ SS V+IL+S I TGDDC+SIGPGT+ LW+E++ACGPGHGISIGSL
Sbjct: 185 ADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 244

Query: 268 GKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQ 327
           GK   E GV+N+TVKTATFTGT+NGVRIKSW RPSNGF +N+ FQH V NNV NPI+IDQ
Sbjct: 245 GKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQ 304

Query: 328 DYCPDDKGCPGQV 340
           +YCP ++ CP QV
Sbjct: 305 NYCPGNENCPNQV 317

BLAST of Spg034994 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 388.7 bits (997), Expect = 5.2e-108
Identity = 188/314 (59.87%), Postives = 240/314 (76.43%), Query Frame = 0

Query: 27  LLLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKG 86
           LL+  V  F +   A    ++++S GAKPDG+TDA+ A  A W +AC SS   TI VPKG
Sbjct: 4   LLVLFVFFFISSCSAQ--SYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKG 63

Query: 87  KFYVQSGDFNG-PCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDG 146
           +F ++S  F+G  CK   +T  IDGTLVA +D +V+   + WI F+HL+G+++ GGVLD 
Sbjct: 64  RFLLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDA 123

Query: 147 QGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKV 206
           +G  +W+CK SGK+CP GAT +    S NV++SGL+S+NSQMF++V+NGC NVK+QGVKV
Sbjct: 124 RGASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKV 183

Query: 207 SAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGS 266
            AAG+SPNTDGIHVQ SS+V+I ++ I TGDDC+SIGPGT+ LW+E++ACGPGHGISIGS
Sbjct: 184 LAAGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGS 243

Query: 267 LGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIID 326
           LGK   E+GV+NVTVKT TFTGT NGVRIKSW RPS+GF +N+ FQH V NNV NPIIID
Sbjct: 244 LGKDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIID 303

Query: 327 QDYCPDDKGCPGQV 340
           Q+YCP D  CP QV
Sbjct: 304 QNYCP-DHDCPRQV 314

BLAST of Spg034994 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 332.0 bits (850), Expect = 5.8e-91
Identity = 160/311 (51.45%), Postives = 218/311 (70.10%), Query Frame = 0

Query: 28  LLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 87
           LL  +L F + +++A   F++VS GAKPDG TD++ A   AW  AC S+ +AT+ VPKG 
Sbjct: 10  LLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGT 69

Query: 88  FYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQG 147
           F ++   F GPCK+  IT  + GT++A  D +    S  WI F  +N  S+ GG  D + 
Sbjct: 70  FLLKVITFGGPCKS-KITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 129

Query: 148 IGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSA 207
            G W+C+ SG++CP G  ++    +++V+ISG+ S+NSQ+ ++ +NGC NV ++ VK+ A
Sbjct: 130 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 189

Query: 208 AGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLG 267
            G+SPNTDG HVQ S+ VT   S++ TGDDC++IGPGT NL +  +ACGPGHG+SIGSL 
Sbjct: 190 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 249

Query: 268 KGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQD 327
           K L+E GVENVTV ++ FTG+QNGVRIKSW RPSNGFVR V FQ +V  NV NPIIIDQ+
Sbjct: 250 KELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQN 309

Query: 328 YCPDDKGCPGQ 339
           YCP  +GCP +
Sbjct: 310 YCPTHEGCPNE 319

BLAST of Spg034994 vs. TAIR 10
Match: AT2G43890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.5e-91
Identity = 163/317 (51.42%), Postives = 222/317 (70.03%), Query Frame = 0

Query: 26  QLLLSLVLLFSNHAL----AAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATI 85
           +L+  L++ FS+  L     A   +++VS GAKPDG TD++ A   AW +AC S+ A T+
Sbjct: 5   KLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAAAVTV 64

Query: 86  YVPKGKFYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGG 145
            VP+G F ++  +F GPC++  IT  I GT+VA SD + L  S  WI F  +N +SI GG
Sbjct: 65  TVPRGSFLLKPVEFRGPCRS-RITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISIIGG 124

Query: 146 VLDGQGIGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQ 205
            LD +G   W C+ SGKSCPVGA ++    + +V++SGL+SINSQ  ++V+N C NV ++
Sbjct: 125 TLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNVIVR 184

Query: 206 GVKVSAAGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGI 265
            VK+ A   SPNTDG+HVQ S+ VT+   +  TGDDCISIGPGT NL+M  + CGPGHGI
Sbjct: 185 KVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPGHGI 244

Query: 266 SIGSLGKGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNP 325
           SIGSLG+   EAGVEN+T+  + F+G+ NGVRIK+W R S GFVRNV+FQ+++  NV NP
Sbjct: 245 SIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNVQNP 304

Query: 326 IIIDQDYCPDDKGCPGQ 339
           II+DQ+YCP ++GCP Q
Sbjct: 305 IIVDQNYCPSNQGCPKQ 320

BLAST of Spg034994 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.3e-90
Identity = 157/309 (50.81%), Postives = 218/309 (70.55%), Query Frame = 0

Query: 28  LLSLVLLFSNHALAAGLKFDIVSLGAKPDGETDASHALQAAWASACGSSEAATIYVPKGK 87
           L+  +L F + + +A + F++VS GAKPDG TD++ A   AW  ACGS+ +AT+ VP G 
Sbjct: 10  LIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTGT 69

Query: 88  FYVQSGDFNGPCKNDAITVVIDGTLVASSDIQVLAKSESWIAFRHLNGLSISGGVLDGQG 147
           F ++   F GPCK+  IT  + GT+VA  D +    S SWI F  +N  S+ GG  D +G
Sbjct: 70  FLLKVITFGGPCKS-KITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDARG 129

Query: 148 IGVWNCKHSGKSCPVGATNLEVLDSQNVMISGLSSINSQMFNIVVNGCQNVKMQGVKVSA 207
            G W+C+ SG++CP G  ++    +++V+ISG+ S+NSQ+ ++ +NGC NV ++ +++ A
Sbjct: 130 SGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRLVA 189

Query: 208 AGDSPNTDGIHVQKSSNVTILSSSIGTGDDCISIGPGTSNLWMESIACGPGHGISIGSLG 267
            GDSPNTDG  VQ S+ VT+  S++ TGDDC++IG GT N  +  +ACGPGHG+SIGSL 
Sbjct: 190 PGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGSLA 249

Query: 268 KGLEEAGVENVTVKTATFTGTQNGVRIKSWGRPSNGFVRNVIFQHIVFNNVNNPIIIDQD 327
           K L E GVENVTV ++ FTG+QNGVRIKSW RPS GFVRNV FQ+++  NV NPIIIDQ+
Sbjct: 250 KQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIIDQN 309

Query: 328 YCPDDKGCP 337
           YCP ++GCP
Sbjct: 310 YCPSNQGCP 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887074.15.9e-13875.94polygalacturonase-like [Benincasa hispida][more]
XP_004140371.15.9e-13877.17polygalacturonase [Cucumis sativus][more]
XP_008460550.19.7e-13373.75PREDICTED: polygalacturonase-like [Cucumis melo][more]
KAA0062404.12.2e-13273.75polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-... [more]
XP_022149030.14.8e-13276.71polygalacturonase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P489791.1e-11564.22Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228182.7e-8551.88Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
Q6H9K05.0e-6344.22Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
P353383.0e-6043.10Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1[more]
P353391.1e-5943.10Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN912.9e-13877.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1[more]
A0A1S3CCA24.7e-13373.75polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1[more]
A0A5D3DS411.1e-13273.75Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A6J1D4L92.3e-13276.71polygalacturonase-like OS=Momordica charantia OX=3673 GN=LOC111017549 PE=3 SV=1[more]
A0A6J1F4894.0e-13271.13polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111440267 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G59850.12.5e-11060.06Pectin lyase-like superfamily protein [more]
AT2G43870.15.2e-10859.87Pectin lyase-like superfamily protein [more]
AT1G05660.15.8e-9151.45Pectin lyase-like superfamily protein [more]
AT2G43890.17.5e-9151.42Pectin lyase-like superfamily protein [more]
AT1G05650.11.3e-9050.81Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 222..243
e-value: 1600.0
score: 5.7
coord: 275..296
e-value: 120.0
score: 11.5
coord: 172..194
e-value: 10000.0
score: 0.0
coord: 305..326
e-value: 3300.0
score: 3.5
coord: 195..221
e-value: 180.0
score: 10.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 34..344
e-value: 5.4E-92
score: 310.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 73..334
e-value: 2.6E-69
score: 233.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 252..265
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 22..339
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 22..339
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 27..340

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg034994.1Spg034994.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098656 anion transmembrane transport
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0015698 inorganic anion transport
cellular_component GO:0005741 mitochondrial outer membrane
molecular_function GO:0004650 polygalacturonase activity
molecular_function GO:0008308 voltage-gated anion channel activity