Spg034662 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg034662 vs. NCBI nr
Match: XP_016652391.1 (PREDICTED: NADH-ubiquinone oxidoreductase chain 2 [Prunus mume])

HSP 1 Score: 509.2 bits (1310), Expect = 3.3e-140
Identity = 287/409 (70.17%), Postives = 290/409 (70.90%), Query Frame = 0

Query: 1   ESLCEPYAVRVARTVTRGVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEIT 60
           ESLCEPYAVRVARTVTRGVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEIT
Sbjct: 87  ESLCEPYAVRVARTVTRGVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEIT 146

Query: 61  GARSSGMFMGILSIAVGFLFKITAVSFRAAVGRTTAYRWKGSTKGGEGKRLRGSRLDFFF 120
           GARSSG+FMGILSIAVGFLFKITAV F                                 
Sbjct: 147 GARSSGIFMGILSIAVGFLFKITAVPF--------------------------------- 206

Query: 121 IKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRWEQQKGRGHRRIRNLWGPIYSISQAPK 180
                                                                       
Sbjct: 207 ------------------------------------------------------------ 266

Query: 181 KTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQ 240
                         +MWAPDIYEGSPTPVTAF SI PKISI ANISRVSIYGSYGATLQQ
Sbjct: 267 --------------HMWAPDIYEGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQ 326

Query: 241 IFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIY 300
           IFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTG SCGTIEGIQSLLIGIFIY
Sbjct: 327 IFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIY 386

Query: 301 VLMTIDAFAIVIALRQTRVTYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFY 360
            LMTIDAFAIV+ALRQTRV YIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFY
Sbjct: 387 ALMTIDAFAIVLALRQTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFY 388

Query: 361 LFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFGRPKAVLHAPDT 410
           LFF ALGCGAYFLAPVGVVTSVIGRWAA RLPRVS FG PKAVL APDT
Sbjct: 447 LFFAALGCGAYFLAPVGVVTSVIGRWAAGRLPRVSQFGGPKAVLRAPDT 388

BLAST of Spg034662 vs. NCBI nr
Match: BBN69459.1 (NADH-Ubiquinone/plastoquinone complex I protein [Prunus dulcis])

HSP 1 Score: 493.0 bits (1268), Expect = 2.4e-135
Identity = 285/428 (66.59%), Postives = 301/428 (70.33%), Query Frame = 0

Query: 26  VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMG-----ILSIAVGFLF 85
           VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG   G     +  I + F+F
Sbjct: 3   VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGSTKGGGRKTLSEIEILFVF 62

Query: 86  KITAVSFRAAVGRTTAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPA 145
                  R A G   AY  +G+     G  + G         +     WP   +   L  
Sbjct: 63  HRKRRRPRMAYG---AYYLRGTRFFDRGSMIAG--------PSPRSARWPIGIAAFGLCL 122

Query: 146 LSHKEFRLGPGKRWEQQKGRG-----HRRIRNLWG------------------------- 205
              K+FRLGPGKRW+QQKGRG      R + + WG                         
Sbjct: 123 PLKKKFRLGPGKRWQQQKGRGPCSCCARPLLSQWGSRVELREQPSDGKLLSRSVQRALFS 182

Query: 206 -----------------------PIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPT 265
                                   IYS+S+APKKTPSPSDS SLL+LYMWAPDIYEGSPT
Sbjct: 183 LRIPRSLSCKFPCNELKTCGALLSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDIYEGSPT 242

Query: 266 PVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL 325
           PVTAF SI PKISI ANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL
Sbjct: 243 PVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL 302

Query: 326 AHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGA 385
           AHSSIGHVGYIRTG SCGTIEGIQSLLIGIFIY LMTIDAFAIV+ALRQTRV YIADLGA
Sbjct: 303 AHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYALMTIDAFAIVLALRQTRVKYIADLGA 362

Query: 386 LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWA 396
           LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSVIGRWA
Sbjct: 363 LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWA 419

BLAST of Spg034662 vs. NCBI nr
Match: CBI23503.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 481.9 bits (1239), Expect = 5.6e-132
Identity = 279/414 (67.39%), Postives = 294/414 (71.01%), Query Frame = 0

Query: 18  GVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVG 77
           GVRVYT SV VGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGILSI VG
Sbjct: 30  GVRVYTYSVRVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGLFMGILSIPVG 89

Query: 78  FLFKITAVSFRAAVGRTTAYRWKGSTKGGEGK-----------------RLRGSRLD--- 137
           FL KITA +     G    Y           K                 R R   LD   
Sbjct: 90  FLLKITAKNRLEVRGPLVPYPTLELAAFAPSKTALVPFLSPFRLLLCFFRFRVYGLDSVS 149

Query: 138 ---------------FFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRWEQQKGRG- 197
                          F    ++  +       LRALPAL +KEFRLGPGKRW+QQKGRG 
Sbjct: 150 PFVALPNERKVVVDHFICFSSKTKKAEDGLRCLRALPALYNKEFRLGPGKRWQQQKGRGP 209

Query: 198 ----HRRIRNLWGPIYSI---------------SQAPKKTPSPSDSISLLSLYMWAPDIY 257
                R + + WG  + I                   +K PSPSDSISLL+LYMWAPDIY
Sbjct: 210 CSCCARPLLSQWGSRFGIYYKRENPLQITTPLLGSPRRKAPSPSDSISLLTLYMWAPDIY 269

Query: 258 EGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 317
           EGSPTPVTAF  I PKISI ANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK
Sbjct: 270 EGSPTPVTAFLFIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 329

Query: 318 VKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYI 377
           VKRPLAHSSIGHVGYIRTG SCGTIEGIQSLLIGIFIY  MTIDAFAIV+AL+QT V YI
Sbjct: 330 VKRPLAHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYASMTIDAFAIVLALQQTCVKYI 389

BLAST of Spg034662 vs. NCBI nr
Match: KAG8362758.1 (hypothetical protein BUALT_BualtUnG0039300 [Buddleja alternifolia] >KAG8363176.1 hypothetical protein BUALT_BualtMtG0005900 [Buddleja alternifolia])

HSP 1 Score: 468.0 bits (1203), Expect = 8.3e-128
Identity = 305/581 (52.50%), Postives = 326/581 (56.11%), Query Frame = 0

Query: 12  ARTVTRGVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGI 71
           AR   R +      VV+GPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGI
Sbjct: 213 ARDGFRSLCASRMRVVLGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGI 272

Query: 72  LSIAVGFLFKITAVSFRAAVGRTTAYR----------------------WKGSTKGGEGK 131
           L IAVGFLFKITAV FRAAVGRT AY                       W G +K   G+
Sbjct: 273 LFIAVGFLFKITAVPFRAAVGRTAAYSCPPRRATRSVVLSHKISTPPACWPGESKFSSGQ 332

Query: 132 ------------------------------------------------------------ 191
                                                                       
Sbjct: 333 LSTQSSAKNTVESRNARCWCASCSQGKKSDKVQFRFDSAQKKNRLEVRGPLVLYSTPEPA 392

Query: 192 -----------------------------------------------RLRGSRLDFFFIK 251
                                                          R R   LD F  +
Sbjct: 393 AFAPSKTALVPFLSPFRLLLCFVPIESKAKWFVCLLYLLDERERTFFRFRVYGLDRFCQR 452

Query: 252 NEEGR-------GWPTD--------SSLRALPALSHKE----------FRLGPGKR---- 311
           N+ G          P+D         S R     + KE          F +   ++    
Sbjct: 453 NQGGARSECFSDPEPSDFACPLQDSGSARESAGNNRKEGVHVAAAPAPFLVNGAQQSRVE 512

Query: 312 WEQQKGRGHRRIRNLWGPIYSIS-------------------------QAPKKTPSPSDS 371
             +Q   G    R++   ++S+                          +  +K PSPSDS
Sbjct: 513 LREQPSDGKLLSRSVQRALFSLRIPRSLSCEFPSGEFKTCGALSIPSLEPRRKPPSPSDS 572

Query: 372 ISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIA 410
           ISLL+LYMWAPDIYEGSPTPVTAF SI PKISI ANISRVSIYGSYGATLQQIFFFCSIA
Sbjct: 573 ISLLTLYMWAPDIYEGSPTPVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIA 632

BLAST of Spg034662 vs. NCBI nr
Match: AZB86935.1 (NADH dehydrogenase subunit 2 [Haberlea rhodopensis])

HSP 1 Score: 466.1 bits (1198), Expect = 3.2e-127
Identity = 266/386 (68.91%), Postives = 269/386 (69.69%), Query Frame = 0

Query: 22  YTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFK 81
           YTCSVV+GPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGIL IAVGFLFK
Sbjct: 27  YTCSVVLGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFK 86

Query: 82  ITAVSFRAAVGRTTAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPAL 141
           ITAV FR                                                     
Sbjct: 87  ITAVPFR----------------------------------------------------- 146

Query: 142 SHKEFRLGPGKRWEQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDI 201
                                                          ISLL+LYMWAPDI
Sbjct: 147 -----------------------------------------------ISLLTLYMWAPDI 206

Query: 202 YEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 261
           YEGSPTPVTAF SI PKISI ANISRVSIY SYGATLQQIFFFCSIASMILGALAAMAQT
Sbjct: 207 YEGSPTPVTAFLSIAPKISISANISRVSIYSSYGATLQQIFFFCSIASMILGALAAMAQT 266

Query: 262 KVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTY 321
           KVKR LAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIY  MTIDAFAIV ALRQTRV Y
Sbjct: 267 KVKRLLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYASMTIDAFAIVSALRQTRVKY 312

Query: 322 IADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTS 381
           IADLGALAKTNPI AITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTS
Sbjct: 327 IADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 312

Query: 382 VIGRWAAERLPRVSPFGRPKAVLHAP 408
           VIGRWAA RLPRVS FG PKAVL AP
Sbjct: 387 VIGRWAAGRLPRVSQFGGPKAVLRAP 312

BLAST of Spg034662 vs. ExPASy Swiss-Prot
Match: O05000 (NADH-ubiquinone oxidoreductase chain 2 OS=Arabidopsis thaliana OX=3702 GN=ND2 PE=1 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 3.5e-105
Identity = 230/374 (61.50%), Postives = 240/374 (64.17%), Query Frame = 0

Query: 35  CSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAVSFRAAVGRT 94
           CSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGIL IAVGFLFKITAV F       
Sbjct: 194 CSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAVPF------- 253

Query: 95  TAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRW 154
                                                                       
Sbjct: 254 ------------------------------------------------------------ 313

Query: 155 EQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFS 214
                                                   +MWAPDIYEGSPTPVTAF S
Sbjct: 314 ----------------------------------------HMWAPDIYEGSPTPVTAFLS 373

Query: 215 IEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 274
           I PKISIFANI RV IYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKR LA+SSIGH
Sbjct: 374 IAPKISIFANILRVFIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRLLAYSSIGH 433

Query: 275 VGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPI 334
           VGYI  GFSCGTIEGIQSLLIGIFIY LMT+DAFAIV+ALRQTRV YIADLGALAKTNPI
Sbjct: 434 VGYICIGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVLALRQTRVKYIADLGALAKTNPI 460

Query: 335 SAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRV 394
            AITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLA VGVVTSVIG +   RL + 
Sbjct: 494 LAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLALVGVVTSVIGCFYYIRLVKR 460

Query: 395 SPFGRPKA-VLHAP 408
             F  P+  +L+ P
Sbjct: 554 MFFDTPRTWILYEP 460

BLAST of Spg034662 vs. ExPASy Swiss-Prot
Match: P93401 (NADH-ubiquinone oxidoreductase chain 2 OS=Oenothera berteroana OX=3950 GN=ND2 PE=2 SV=2)

HSP 1 Score: 380.2 bits (975), Expect = 3.0e-104
Identity = 228/374 (60.96%), Postives = 239/374 (63.90%), Query Frame = 0

Query: 35  CSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAVSFRAAVGRT 94
           CSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGIL IAVGFLFKITAV F       
Sbjct: 183 CSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAVPF------- 242

Query: 95  TAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRW 154
                                                                       
Sbjct: 243 ------------------------------------------------------------ 302

Query: 155 EQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFS 214
                                                   +MWAPDIYEGSPTPVTAF S
Sbjct: 303 ----------------------------------------HMWAPDIYEGSPTPVTAFLS 362

Query: 215 IEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 274
           I PKISIFANI RV IYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKR LA+SSIGH
Sbjct: 363 IAPKISIFANILRVFIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRLLAYSSIGH 422

Query: 275 VGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPI 334
           VGYI  GFSCGTIEGIQSLLIG+FIY L TI+AFAIV+ALRQTRV YIADLGALAKTNPI
Sbjct: 423 VGYICIGFSCGTIEGIQSLLIGLFIYALTTINAFAIVLALRQTRVKYIADLGALAKTNPI 449

Query: 335 SAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRV 394
            AITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLA VGVVTSVIG +   RL + 
Sbjct: 483 LAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLASVGVVTSVIGCFYYIRLVKR 449

Query: 395 SPFGRPKA-VLHAP 408
             F  P+  +L+ P
Sbjct: 543 MFFDTPRTWILYEP 449

BLAST of Spg034662 vs. ExPASy Swiss-Prot
Match: P26846 (NADH-ubiquinone oxidoreductase chain 2 OS=Marchantia polymorpha OX=3197 GN=ND2 PE=3 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 5.0e-91
Identity = 206/376 (54.79%), Postives = 225/376 (59.84%), Query Frame = 0

Query: 35  CSMIYGSTGATHFDQLAKILTGYEIT--GARSSGMFMGILSIAVGFLFKITAVSFRAAVG 94
           CSMIYG TG T+F++LAKI TGYEIT  GA+SSG+FMGIL IAVGFLFKITAV F     
Sbjct: 182 CSMIYGFTGVTNFEELAKIFTGYEITLFGAQSSGIFMGILFIAVGFLFKITAVPF----- 241

Query: 95  RTTAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGK 154
                                                                       
Sbjct: 242 ------------------------------------------------------------ 301

Query: 155 RWEQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAF 214
                                                     +MWAPD+YEGSPT VTAF
Sbjct: 302 ------------------------------------------HMWAPDVYEGSPTIVTAF 361

Query: 215 FSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSI 274
           FSI PKISI AN+ RV IY  Y  T QQ+FFFCSIASMILGALAAMAQ KVKR LA+SSI
Sbjct: 362 FSIAPKISILANMLRVFIYSFYDPTWQQLFFFCSIASMILGALAAMAQNKVKRLLAYSSI 421

Query: 275 GHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTN 334
           GHVGY+  GFSCGTIEGIQSLLIGIFIYVLMT++ FAIV+ALRQ R  YIADLGALAKTN
Sbjct: 422 GHVGYLLIGFSCGTIEGIQSLLIGIFIYVLMTVNVFAIVLALRQNRFKYIADLGALAKTN 450

Query: 335 PISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLP 394
           PI AIT SITMFSYAGIPPLAGFCSKFYLFF ALGCGAY LA +GVVTSVI  +   R  
Sbjct: 482 PILAITLSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYLLALIGVVTSVISCFYYIRFV 450

Query: 395 RVSPFGRPKA-VLHAP 408
           ++  F  PK  VL+ P
Sbjct: 542 KIMYFDTPKTWVLYKP 450

BLAST of Spg034662 vs. ExPASy Swiss-Prot
Match: P15688 (NADH-ubiquinone oxidoreductase chain 2 OS=Beta vulgaris OX=161934 GN=ND2 PE=3 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 9.1e-77
Identity = 169/238 (71.01%), Postives = 177/238 (74.37%), Query Frame = 0

Query: 164 RIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFA 223
           RI+N WGP    S +P KTP P         YMWAPDIYEGSPTPVTAFFSI P+ SI A
Sbjct: 187 RIQNFWGPSIP-SSSPAKTPLPFGLNIFFDSYMWAPDIYEGSPTPVTAFFSIAPERSISA 246

Query: 224 NISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFS 283
           NI RV IYGSYGATLQQIFFFCSIA + L +  AMA        A SSIG + Y    F 
Sbjct: 247 NILRVFIYGSYGATLQQIFFFCSIA-LRLRSTGAMA----NEGKASSSIGQLDYGGLYFV 306

Query: 284 CGTI---EGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPISAITFS 343
              +   EGIQSLLIG+FIY  M    FAIV ALRQTRV YIADLGALAKTNPISAITFS
Sbjct: 307 LVLMWNREGIQSLLIGLFIYASMDDRCFAIVSALRQTRVKYIADLGALAKTNPISAITFS 366

Query: 344 ITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFG 399
           ITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSVIG WAA RLPRVS FG
Sbjct: 367 ITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGCWAAGRLPRVSQFG 418

BLAST of Spg034662 vs. ExPASy Swiss-Prot
Match: A8I421 (NADH-quinone oxidoreductase subunit N OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=nuoN PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.9e-44
Identity = 99/216 (45.83%), Postives = 140/216 (64.81%), Query Frame = 0

Query: 189 ISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGAT--LQQIFFFCS 248
           +S +  +MW PD+YEG+PTPVTAFF+  PK++  A   RV++      T   QQI  F S
Sbjct: 217 VSAVPFHMWTPDVYEGAPTPVTAFFATAPKVAAMAVFVRVAVEALPHVTHSWQQIVTFVS 276

Query: 249 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTID 308
           IASM LGA AA+ Q  +KR LA+SSIGH+G+   G + GT +G+  +L+ + IYV+MT+ 
Sbjct: 277 IASMALGAFAAIGQRNIKRLLAYSSIGHMGFALVGLAAGTEQGVTGVLLYMAIYVVMTLG 336

Query: 309 AFAIVIALRQ--TRVTYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFT 368
           +F  V+A+R+    V  I DL  LA+T P+ A+  +  MFS AGIPPLAGF +K+Y+F  
Sbjct: 337 SFTCVLAMRRDGRSVETIEDLAGLARTKPLMALALAALMFSLAGIPPLAGFVAKYYVFLA 396

Query: 369 ALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFGRP 401
           A+  G Y LA +GVV SV+G +   R+ ++  F  P
Sbjct: 397 AIQAGLYGLAVIGVVASVVGAYYYLRVVKIMYFDEP 432

BLAST of Spg034662 vs. ExPASy TrEMBL
Match: F6H891 (Proton_antipo_M domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_00s0505g00030 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 2.2e-142
Identity = 297/444 (66.89%), Postives = 315/444 (70.95%), Query Frame = 0

Query: 18  GVRVYTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVG 77
           GVRVYT SV VGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGILSI VG
Sbjct: 30  GVRVYTYSVRVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGLFMGILSIPVG 89

Query: 78  FLFKITAVSFRAAVGRTTAYRWKGSTKGGEGK-----------------RLRGSRLD--- 137
           FL KITA +     G    Y           K                 R R   LD   
Sbjct: 90  FLLKITAKNRLEVRGPLVPYPTLELAAFAPSKTALVPFLSPFRLLLCFFRFRVYGLDSVS 149

Query: 138 ---------------FFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRWEQQKGRG- 197
                          F    ++  +       LRALPAL +KEFRLGPGKRW+QQKGRG 
Sbjct: 150 PFVALPNERKVVVDHFICFSSKTKKAEDGLRCLRALPALYNKEFRLGPGKRWQQQKGRGP 209

Query: 198 ----HRRIRNLWGPIYSI---------------SQAPKKTPSPSDSISLLSLYMWAPDIY 257
                R + + WG  + I                   +K PSPSDSISLL+LYMWAPDIY
Sbjct: 210 CSCCARPLLSQWGSRFGIYYKRENPLQITTPLLGSPRRKAPSPSDSISLLTLYMWAPDIY 269

Query: 258 EGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 317
           EGSPTPVTAF  I PKISI ANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK
Sbjct: 270 EGSPTPVTAFLFIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTK 329

Query: 318 VKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYI 377
           VKRPLAHSSIGHVGYIRTG SCGTIEGIQSLLIGIFIY  MTIDAFAIV+AL+QT V YI
Sbjct: 330 VKRPLAHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYASMTIDAFAIVLALQQTCVKYI 389

Query: 378 ADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSV 407
           ADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSV
Sbjct: 390 ADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSV 449

BLAST of Spg034662 vs. ExPASy TrEMBL
Match: A0A5H2XRW6 (NADH-Ubiquinone/plastoquinone complex I protein OS=Prunus dulcis OX=3755 GN=Prudu_950S000900 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.2e-135
Identity = 285/428 (66.59%), Postives = 301/428 (70.33%), Query Frame = 0

Query: 26  VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMG-----ILSIAVGFLF 85
           VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG   G     +  I + F+F
Sbjct: 3   VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGSTKGGGRKTLSEIEILFVF 62

Query: 86  KITAVSFRAAVGRTTAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPA 145
                  R A G   AY  +G+     G  + G         +     WP   +   L  
Sbjct: 63  HRKRRRPRMAYG---AYYLRGTRFFDRGSMIAG--------PSPRSARWPIGIAAFGLCL 122

Query: 146 LSHKEFRLGPGKRWEQQKGRG-----HRRIRNLWG------------------------- 205
              K+FRLGPGKRW+QQKGRG      R + + WG                         
Sbjct: 123 PLKKKFRLGPGKRWQQQKGRGPCSCCARPLLSQWGSRVELREQPSDGKLLSRSVQRALFS 182

Query: 206 -----------------------PIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPT 265
                                   IYS+S+APKKTPSPSDS SLL+LYMWAPDIYEGSPT
Sbjct: 183 LRIPRSLSCKFPCNELKTCGALLSIYSLSRAPKKTPSPSDSTSLLTLYMWAPDIYEGSPT 242

Query: 266 PVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL 325
           PVTAF SI PKISI ANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL
Sbjct: 243 PVTAFLSIAPKISISANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPL 302

Query: 326 AHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGA 385
           AHSSIGHVGYIRTG SCGTIEGIQSLLIGIFIY LMTIDAFAIV+ALRQTRV YIADLGA
Sbjct: 303 AHSSIGHVGYIRTGLSCGTIEGIQSLLIGIFIYALMTIDAFAIVLALRQTRVKYIADLGA 362

Query: 386 LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWA 396
           LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSVIGRWA
Sbjct: 363 LAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRWA 419

BLAST of Spg034662 vs. ExPASy TrEMBL
Match: A0A4D8YAJ0 (NADH-quinone oxidoreductase subunit N OS=Salvia splendens OX=180675 GN=nuoN PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.9e-130
Identity = 293/488 (60.04%), Postives = 309/488 (63.32%), Query Frame = 0

Query: 26  VVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAV 85
           VV+GPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGIL IAVGFLFKITAV
Sbjct: 3   VVLGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFKITAV 62

Query: 86  SFRAAVGRTTAYR--------------------------------------WKGSTKGGE 145
            FRAAVGRT AYR                                      W G +K   
Sbjct: 63  PFRAAVGRTAAYRCMLDESAAFSVVLSHSCPPRRATRSVVLSHKISTPPACWPGESKFSS 122

Query: 146 G-------------------------KRLRGSRLDFFFIKNEEGRGWPTDS--------- 205
           G                         +  +  ++ F F   + G   P  S         
Sbjct: 123 GQLSTQSSAKNTVESRNARCWCASCSQGKKSDKVQFRFDCLQHGSRLPKKSLGTMKKKDI 182

Query: 206 --SLRALPALSHKEFRLGPGKRWEQ------------QKGRGHRRIRNLWGPIYSISQAP 265
             S  A P       R   G   ++              G   R + +L  P     + P
Sbjct: 183 SPSGFACPLQDSGSARESAGNNRKEGVHVAAAPAPFLVNGAQQRALFSLRIPRSLSCEFP 242

Query: 266 ------------------KKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISI 325
                             +K PSPSDSISLL+LYMWAPDIYEGSPTPVTAF SI PKISI
Sbjct: 243 SGEFKTCGALSIPSLEPRRKPPSPSDSISLLTLYMWAPDIYEGSPTPVTAFLSIAPKISI 302

Query: 326 FANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTG 385
            ANISR+SIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKR LAHSSIGHVGYIR G
Sbjct: 303 SANISRLSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRLLAHSSIGHVGYIRIG 362

Query: 386 FSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPISAITFSI 410
           FSCGTIEGIQSLLIGIFIY  MTIDAFAIV ALRQTRV YIADLGALAKTNPI AITFSI
Sbjct: 363 FSCGTIEGIQSLLIGIFIYASMTIDAFAIVSALRQTRVKYIADLGALAKTNPILAITFSI 422

BLAST of Spg034662 vs. ExPASy TrEMBL
Match: A0A251UAH0 (Putative NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit OS=Helianthus annuus OX=4232 GN=HannXRQ_Chr07g0188931 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 3.1e-128
Identity = 267/362 (73.76%), Postives = 283/362 (78.18%), Query Frame = 0

Query: 47  FDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAVSFRAAVGRTTAYRWKGSTKGG 106
           F QLAKILTGYEITGA+SSG+FMGILSIAVG LFKITAV FR AVGRT+AYR  G+++  
Sbjct: 9   FYQLAKILTGYEITGAQSSGIFMGILSIAVGSLFKITAVPFRTAVGRTSAYRCCGNSRLM 68

Query: 107 EGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRWEQQKGRGHRRIR 166
           E         DF     E+        SLR   +LS  +                 RRI+
Sbjct: 69  EN--------DFHIWFGEQ------FFSLRIPRSLSIDQ-----------------RRIQ 128

Query: 167 NLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANIS 226
           NLWGPIYSIS+APK TPS SDSISLL+LYMWAPDIYEGSPTPVTAF SI PKISI ANIS
Sbjct: 129 NLWGPIYSISRAPKITPSSSDSISLLTLYMWAPDIYEGSPTPVTAFLSIAPKISISANIS 188

Query: 227 RVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGT 286
           R+ IYGSYGATLQQIFFFCSIASMILGALAAMA TKVKR LAHSSIGHVGYIRT FSCGT
Sbjct: 189 RLYIYGSYGATLQQIFFFCSIASMILGALAAMAPTKVKRLLAHSSIGHVGYIRTCFSCGT 248

Query: 287 IEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPISAITFSITMFSY 346
           IEGIQSLLIGIFIY  MTIDAFAIV ALRQ+ V YIADL ALAKTNPISAITFS TMFSY
Sbjct: 249 IEGIQSLLIGIFIYASMTIDAFAIVSALRQSLVKYIADLDALAKTNPISAITFSNTMFSY 308

Query: 347 AGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFGRPKAVLHA 406
           AG P L+GFCSKFYLFF ALGCGAYFLA VG+VT VIGRWA  RLPRVS FG PKAVL  
Sbjct: 309 AGTPSLSGFCSKFYLFFAALGCGAYFLALVGIVTRVIGRWAVGRLPRVSQFGGPKAVLRG 339

Query: 407 PD 409
           PD
Sbjct: 369 PD 339

BLAST of Spg034662 vs. ExPASy TrEMBL
Match: A0A3G6XMH9 (NADH dehydrogenase subunit 2 OS=Haberlea rhodopensis OX=121482 GN=nad2 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 1.5e-127
Identity = 266/386 (68.91%), Postives = 269/386 (69.69%), Query Frame = 0

Query: 22  YTCSVVVGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFK 81
           YTCSVV+GPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGIL IAVGFLFK
Sbjct: 27  YTCSVVLGPTHPICSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILFIAVGFLFK 86

Query: 82  ITAVSFRAAVGRTTAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPAL 141
           ITAV FR                                                     
Sbjct: 87  ITAVPFR----------------------------------------------------- 146

Query: 142 SHKEFRLGPGKRWEQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDI 201
                                                          ISLL+LYMWAPDI
Sbjct: 147 -----------------------------------------------ISLLTLYMWAPDI 206

Query: 202 YEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQT 261
           YEGSPTPVTAF SI PKISI ANISRVSIY SYGATLQQIFFFCSIASMILGALAAMAQT
Sbjct: 207 YEGSPTPVTAFLSIAPKISISANISRVSIYSSYGATLQQIFFFCSIASMILGALAAMAQT 266

Query: 262 KVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTY 321
           KVKR LAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIY  MTIDAFAIV ALRQTRV Y
Sbjct: 267 KVKRLLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYASMTIDAFAIVSALRQTRVKY 312

Query: 322 IADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTS 381
           IADLGALAKTNPI AITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTS
Sbjct: 327 IADLGALAKTNPILAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTS 312

Query: 382 VIGRWAAERLPRVSPFGRPKAVLHAP 408
           VIGRWAA RLPRVS FG PKAVL AP
Sbjct: 387 VIGRWAAGRLPRVSQFGGPKAVLRAP 312

BLAST of Spg034662 vs. TAIR 10
Match: ATMG00285.1 (NADH dehydrogenase 2A )

HSP 1 Score: 404.1 bits (1037), Expect = 1.4e-112
Identity = 238/374 (63.64%), Postives = 246/374 (65.78%), Query Frame = 0

Query: 35  CSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAVSFRAAVGRT 94
           CSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGILSIAVGFLFKITAV F       
Sbjct: 194 CSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILSIAVGFLFKITAVPF------- 253

Query: 95  TAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRW 154
                                                                       
Sbjct: 254 ------------------------------------------------------------ 313

Query: 155 EQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFS 214
                                                   +MWAPDIYEGSPTPVTAF S
Sbjct: 314 ----------------------------------------HMWAPDIYEGSPTPVTAFLS 373

Query: 215 IEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 274
           I PKISI ANI RVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH
Sbjct: 374 IAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 433

Query: 275 VGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPI 334
           VGYIRTGFSCGTIEGIQSLLIGIFIY LMT+DAFAIV ALRQTRV YIADLGALAKTNPI
Sbjct: 434 VGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALRQTRVKYIADLGALAKTNPI 460

Query: 335 SAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRV 394
           SAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSVIGR+   RL + 
Sbjct: 494 SAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRFYYIRLVKR 460

Query: 395 SPFGRPKA-VLHAP 408
             F  P+  +L+ P
Sbjct: 554 MFFDTPRTWILYEP 460

BLAST of Spg034662 vs. TAIR 10
Match: ATMG01320.1 (NADH dehydrogenase 2B )

HSP 1 Score: 404.1 bits (1037), Expect = 1.4e-112
Identity = 238/374 (63.64%), Postives = 246/374 (65.78%), Query Frame = 0

Query: 35  CSMIYGSTGATHFDQLAKILTGYEITGARSSGMFMGILSIAVGFLFKITAVSFRAAVGRT 94
           CSMIYGSTGATHFDQLAKILTGYEITGARSSG+FMGILSIAVGFLFKITAV F       
Sbjct: 194 CSMIYGSTGATHFDQLAKILTGYEITGARSSGIFMGILSIAVGFLFKITAVPF------- 253

Query: 95  TAYRWKGSTKGGEGKRLRGSRLDFFFIKNEEGRGWPTDSSLRALPALSHKEFRLGPGKRW 154
                                                                       
Sbjct: 254 ------------------------------------------------------------ 313

Query: 155 EQQKGRGHRRIRNLWGPIYSISQAPKKTPSPSDSISLLSLYMWAPDIYEGSPTPVTAFFS 214
                                                   +MWAPDIYEGSPTPVTAF S
Sbjct: 314 ----------------------------------------HMWAPDIYEGSPTPVTAFLS 373

Query: 215 IEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 274
           I PKISI ANI RVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH
Sbjct: 374 IAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGALAAMAQTKVKRPLAHSSIGH 433

Query: 275 VGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALRQTRVTYIADLGALAKTNPI 334
           VGYIRTGFSCGTIEGIQSLLIGIFIY LMT+DAFAIV ALRQTRV YIADLGALAKTNPI
Sbjct: 434 VGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALRQTRVKYIADLGALAKTNPI 460

Query: 335 SAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAPVGVVTSVIGRWAAERLPRV 394
           SAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAPVGVVTSVIGR+   RL + 
Sbjct: 494 SAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAPVGVVTSVIGRFYYIRLVKR 460

Query: 395 SPFGRPKA-VLHAP 408
             F  P+  +L+ P
Sbjct: 554 MFFDTPRTWILYEP 460

BLAST of Spg034662 vs. TAIR 10
Match: AT2G07689.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 385.6 bits (989), Expect = 5.1e-107
Identity = 200/214 (93.46%), Postives = 202/214 (94.39%), Query Frame = 0

Query: 196 MWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQQIFFFCSIASMILGAL 255
           MWAPDIYEGSPTPVTAF SI PKISI ANI RVSIYGSYGATLQQIFFFCSIASMILGAL
Sbjct: 1   MWAPDIYEGSPTPVTAFLSIAPKISISANILRVSIYGSYGATLQQIFFFCSIASMILGAL 60

Query: 256 AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYVLMTIDAFAIVIALR 315
           AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIY LMT+DAFAIV ALR
Sbjct: 61  AAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIEGIQSLLIGIFIYALMTMDAFAIVSALR 120

Query: 316 QTRVTYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFTALGCGAYFLAP 375
           QTRV YIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFF ALGCGAYFLAP
Sbjct: 121 QTRVKYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFAALGCGAYFLAP 180

Query: 376 VGVVTSVIGRWAAERLPRVSPFGRPKAVLHAPDT 410
           VGVVTSVIGRWAA RLPR+S FG PKAVL APDT
Sbjct: 181 VGVVTSVIGRWAAGRLPRISKFGGPKAVLRAPDT 214

BLAST of Spg034662 vs. TAIR 10
Match: ATCG00890.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.7e-28
Identity = 75/219 (34.25%), Postives = 121/219 (55.25%), Query Frame = 0

Query: 189 ISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQ--QIFFFCS 248
           +SL   + W PD+YEGSPTPV AF S+  K++  A+ +R+     Y ++ +   +    +
Sbjct: 121 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDIPFYFSSNEWHLLLEILA 180

Query: 249 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIE-GIQSLLIGIFIYVLMTI 308
           I SMI G L A+ QT +KR LA+SSIG +GY+  G   G    G  S++  +  Y+ M +
Sbjct: 181 ILSMIFGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNGGYASMITYMLFYIAMNL 240

Query: 309 DAFAIVIAL-RQTRVTYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFT 368
             FA +I    +T    I D   L   +P  A++ ++ + S  G+PPLAGF  K +LF+ 
Sbjct: 241 GTFACIILFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWC 300

Query: 369 ALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFGRPKAV 404
               G YFL  +G++TSV+  +   ++ ++   GR + +
Sbjct: 301 GWQAGLYFLVSIGLLTSVLSIYYYLKIIKLLMTGRNQEI 339

BLAST of Spg034662 vs. TAIR 10
Match: ATCG01250.1 (NADH-Ubiquinone/plastoquinone (complex I) protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.7e-28
Identity = 75/219 (34.25%), Postives = 121/219 (55.25%), Query Frame = 0

Query: 189 ISLLSLYMWAPDIYEGSPTPVTAFFSIEPKISIFANISRVSIYGSYGATLQ--QIFFFCS 248
           +SL   + W PD+YEGSPTPV AF S+  K++  A+ +R+     Y ++ +   +    +
Sbjct: 121 LSLAPSHQWTPDVYEGSPTPVVAFLSVTSKVAASASATRIFDIPFYFSSNEWHLLLEILA 180

Query: 249 IASMILGALAAMAQTKVKRPLAHSSIGHVGYIRTGFSCGTIE-GIQSLLIGIFIYVLMTI 308
           I SMI G L A+ QT +KR LA+SSIG +GY+  G   G    G  S++  +  Y+ M +
Sbjct: 181 ILSMIFGNLIAITQTSMKRMLAYSSIGQIGYVIIGIIVGDSNGGYASMITYMLFYIAMNL 240

Query: 309 DAFAIVIAL-RQTRVTYIADLGALAKTNPISAITFSITMFSYAGIPPLAGFCSKFYLFFT 368
             FA +I    +T    I D   L   +P  A++ ++ + S  G+PPLAGF  K +LF+ 
Sbjct: 241 GTFACIILFGLRTGTDNIRDYAGLYTKDPFLALSLALCLLSLGGLPPLAGFFGKLHLFWC 300

Query: 369 ALGCGAYFLAPVGVVTSVIGRWAAERLPRVSPFGRPKAV 404
               G YFL  +G++TSV+  +   ++ ++   GR + +
Sbjct: 301 GWQAGLYFLVSIGLLTSVLSIYYYLKIIKLLMTGRNQEI 339