Spg034147 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034147
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	vignain-like
Location	scaffold13: 36319791 .. 36321221 (-)
RNA-Seq Expression	Spg034147
Synteny	Spg034147

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATTCCCAAATTTCTTTTGGTTTTTCTTCTTTTGATTGTTCTGGTTTCAGGATTGGCCAAGAGCTTTGAATTTGATGAGAAGGAGTTAGCAACGGAAGAAAGTCTATGGAAGTTGTATGAGAGGTGGAGTCACCACCACACAATATCAAGAGACCTAAAGGAGAAGCATAAGCGTTTCGACGTATTCAAAGAGAACGTGAACCATGTATTCACAGTGAATCAAATGAACAAACCGTACAAGTTGAAGTTGAACAAGTTTGCAGATATGTCCAACTATGAGTTTGTGAACTTATATGCTCGGTCCAATATTAGCCACTACAGGAAGTTACATGGTAGGAAAAGAGGAGGTGTCGGTGGATTCATGTATGAACAGGTTACGGATCTTCCAGCAGCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGGCATCAAGGAGCAAGGCAGATGCGGTAAATAATGTTTAATTTTGGCGTTTGTATTGATTGAGGGTAAAAAAAGTTTATAACTAAAAACAACATTTAATAGGTTTGAGTGTTCTATCCTACTAGATTAGGACGAATTACTGCTTACAATGTCAATGAGAAGCACTAGAATATACTTTAATTTTGAAATGACGATTAATCAATAACTTTGAAATATATTCTAGGTAGCTGTTGGGCATTTTCAACGGTGGCGGCAGTTGAAGGAATCAACAAAATCAAAACCAAACAGCTATTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATACAAGGAACAGTGGCTGTAACGGAGGATTCATGGAGATTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTGCCACTGAGAACAACTATCCTTACCACGGTGCAAGAGGATTTTGCCGCTCATCGAGAGTTAGTTTTAGAATTCATCTAACTTCATGAAGCAATTCAAATCTAAAATATCATTCATCATTTCGGAAATTATCAAACTAAACACATATTTATTTGATTTAGCAGATCACTTCACCGATAGTTACAATTGATGGATACGAAAACGTACCTATAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCGCCATCGACGCCGCAGGAAAAGATTTCCAATTCTACTCAGAGGTTAGCTCCTAGATTCAGTTTCACAGTTGATCGCTTTCACTGTGAACTTGCATCATATCCTAATCGAAAATTTGTGGCTAAATTGCAGGGGGTATTCGATGGAGAATGTGGAACAGAGCTTAATCACGGAGTGGTGGCGATTGGCTATGGAACAACTGAAGACGGAACAGATTACTGGTTGGTGAGGAATTCATGGGGAGTCGGTTGGGGAGAGGAAGGTTACGTGAGGATGAAAAAAGGAGTGGAGGATCCCGAAGGAATGTGTGGAATAGCCATGGAAGCCTCTTATCCAATCAAGTTCTAA

mRNA sequence

ATGGCCATTCCCAAATTTCTTTTGGTTTTTCTTCTTTTGATTGTTCTGGTTTCAGGATTGGCCAAGAGCTTTGAATTTGATGAGAAGGAGTTAGCAACGGAAGAAAGTCTATGGAAGTTGTATGAGAGGTGGAGTCACCACCACACAATATCAAGAGACCTAAAGGAGAAGCATAAGCGTTTCGACGTATTCAAAGAGAACGTGAACCATGTATTCACAGTGAATCAAATGAACAAACCGTACAAGTTGAAGTTGAACAAGTTTGCAGATATGTCCAACTATGAGTTTGTGAACTTATATGCTCGGTCCAATATTAGCCACTACAGGAAGTTACATGGTAGGAAAAGAGGAGGTGTCGGTGGATTCATGTATGAACAGGTTACGGATCTTCCAGCAGCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGGCATCAAGGAGCAAGGCAGATGCGGTAGCTGTTGGGCATTTTCAACGGTGGCGGCAGTTGAAGGAATCAACAAAATCAAAACCAAACAGCTATTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATACAAGGAACAGTGGCTGTAACGGAGGATTCATGGAGATTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTGCCACTGAGAACAACTATCCTTACCACGGTGCAAGAGGATTTTGCCGCTCATCGAGAGTTATTACAATTGATGGATACGAAAACGTACCTATAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCGCCATCGACGCCGCAGGAAAAGATTTCCAATTCTACTCAGAGGGGGTATTCGATGGAGAATGTGGAACAGAGCTTAATCACGGAGTGGTGGCGATTGGCTATGGAACAACTGAAGACGGAACAGATTACTGGTTGGTGAGGAATTCATGGGGAGTCGGTTGGGGAGAGGAAGGTTACGTGAGGATGAAAAAAGGAGTGGAGGATCCCGAAGGAATGTGTGGAATAGCCATGGAAGCCTCTTATCCAATCAAGTTCTAA

Coding sequence (CDS)

ATGGCCATTCCCAAATTTCTTTTGGTTTTTCTTCTTTTGATTGTTCTGGTTTCAGGATTGGCCAAGAGCTTTGAATTTGATGAGAAGGAGTTAGCAACGGAAGAAAGTCTATGGAAGTTGTATGAGAGGTGGAGTCACCACCACACAATATCAAGAGACCTAAAGGAGAAGCATAAGCGTTTCGACGTATTCAAAGAGAACGTGAACCATGTATTCACAGTGAATCAAATGAACAAACCGTACAAGTTGAAGTTGAACAAGTTTGCAGATATGTCCAACTATGAGTTTGTGAACTTATATGCTCGGTCCAATATTAGCCACTACAGGAAGTTACATGGTAGGAAAAGAGGAGGTGTCGGTGGATTCATGTATGAACAGGTTACGGATCTTCCAGCAGCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGGCATCAAGGAGCAAGGCAGATGCGGTAGCTGTTGGGCATTTTCAACGGTGGCGGCAGTTGAAGGAATCAACAAAATCAAAACCAAACAGCTATTATCTCTATCAGAGCAGGAGCTGCTCGACTGCAATACAAGGAACAGTGGCTGTAACGGAGGATTCATGGAGATTGCTTTTGATTTCATAAGGCAAAATGGTGGAATTGCCACTGAGAACAACTATCCTTACCACGGTGCAAGAGGATTTTGCCGCTCATCGAGAGTTATTACAATTGATGGATACGAAAACGTACCTATAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCGCCATCGACGCCGCAGGAAAAGATTTCCAATTCTACTCAGAGGGGGTATTCGATGGAGAATGTGGAACAGAGCTTAATCACGGAGTGGTGGCGATTGGCTATGGAACAACTGAAGACGGAACAGATTACTGGTTGGTGAGGAATTCATGGGGAGTCGGTTGGGGAGAGGAAGGTTACGTGAGGATGAAAAAAGGAGTGGAGGATCCCGAAGGAATGTGTGGAATAGCCATGGAAGCCTCTTATCCAATCAAGTTCTAA

Protein sequence

MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRVITIDGYENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF

Homology

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BLAST of Spg034147 vs. NCBI nr
Match: XP_038885064.1 (LOW QUALITY PROTEIN: vignain-like [Benincasa hispida])

HSP 1 Score: 625.9 bits (1613), Expect = 2.0e-175
Identity = 300/346 (86.71%), Postives = 322/346 (93.06%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLL+ LLLIVL+SG A+SFEFDEKELATEESLWKLYERW +HHTISRDLKEKHKR
Sbjct: 29  MAIIKFLLLPLLLIVLISGFAESFEFDEKELATEESLWKLYERWGNHHTISRDLKEKHKR 88

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F VFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVN YARSNISHYRKLHGR+R G  
Sbjct: 89  FKVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHGRRREGTS 148

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYE+ TDLP+ IDWRERGAVNGIKEQGRCGSCWAFS+VAAVEGINKI+T QLLSLSEQ
Sbjct: 149 GFMYEEATDLPSYIDWRERGAVNGIKEQGRCGSCWAFSSVAAVEGINKIRTNQLLSLSEQ 208

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFIR+NGGIATEN+YPYHG+RG CRSSR    ++ IDGY
Sbjct: 209 ELLDCNYRNEGCNGGFMEIAFDFIRRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 268

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E++P NE+ALMQAVANQPVSVAIDAAG+DFQFY +GVFDG CGTELNHGVVAIGYGTTED
Sbjct: 269 ESIPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 328

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYW+VRNSWGVGWGEEGYVRMK+GVE PEG+CGIAMEASYPIKF
Sbjct: 329 GTDYWIVRNSWGVGWGEEGYVRMKRGVEQPEGLCGIAMEASYPIKF 374

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BLAST of Spg034147 vs. NCBI nr
Match: XP_008444390.1 (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 616.7 bits (1589), Expect = 1.2e-172
Identity = 298/346 (86.13%), Postives = 322/346 (93.06%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLLV LLLIVLVSGLA+SFEFDEKELATEESLW+LYERW +HHTISR+LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F VFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVN YARSNISH+RKLHGR+R G G
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+++DWRERGAVN IKEQG CGSCWAFS+VAAVE INKIKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATEN+YPYHG+RG CRSSR    ++ IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NE+ALMQAVANQPVSVAIDAAG+DFQFY +GVFDG CGTELNHGVVAIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYW+VRNSWGVGWGE+GYVRMK+GVE PEG+CGIAMEASYPIKF
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

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BLAST of Spg034147 vs. NCBI nr
Match: XP_004142960.1 (vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis sativus])

HSP 1 Score: 615.9 bits (1587), Expect = 2.1e-172
Identity = 298/346 (86.13%), Postives = 322/346 (93.06%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLLV LLLIVLVSGLA+SFEFDEKELATEESLW+LYERW  HHTISR+LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F VFKENVNHVFTVNQM+KPYKLKLNKFADMSNYEFVN YARSNISHYRKLH R+R G G
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRR-GAG 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+++DWRERGAVN +KEQGRCGSCWAFS+VAAVEGINKIKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATEN+YPYHG+RG CRSSR    ++ IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NE+ALMQAVANQPVSVAIDAAG+DFQFYS+GVFDG CGTELNHGVVAIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYWLVRNSWGVGWGE+GYVRMK+GVE  EG+CGIAMEASYPIK+
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

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BLAST of Spg034147 vs. NCBI nr
Match: XP_022996765.1 (vignain-like [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 3.2e-165
Identity = 294/376 (78.19%), Postives = 317/376 (84.31%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KF+LV  LLIVLVSGL +SFEFDEKELATEESLW+LYERW +HHTISR LKEKHKR
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F+VFKENVNHVFTVNQMNKPYKLKLNKFADMSN EFV+ YARSNISHYRKLHG++    G
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRS---G 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+ IDWRERGAVN IKEQG+CGSCWAFSTVAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRV----ITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATENNYPYHGARG CRSSR+    +TIDGY
Sbjct: 181 ELLDCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYS-------------------------- 300
           E+VP NENALMQAVANQPVSVAIDA G+DFQFYS                          
Sbjct: 241 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKS 300

Query: 301 ----EGVFDGECGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDP 343
               +GVFDG CGTELNHGVVAIGYGTTE+GTDYW+VRNSWGVGWGEEGYVRMK+GVE  
Sbjct: 301 VGKVQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKA 360

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BLAST of Spg034147 vs. NCBI nr
Match: XP_023545499.1 (vignain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590.9 bits (1522), Expect = 7.1e-165
Identity = 294/375 (78.40%), Postives = 316/375 (84.27%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLLV  LLIVLVSGL +SFEFDEKELATEESLW+LYERW +HHTISR LKEKHKR
Sbjct: 28  MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 87

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F+VFKENVNHVFTVNQMNKPYKLKLNKFADMSN EFV+ YARSNISHYRKLHG++    G
Sbjct: 88  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRS---G 147

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+ IDWRERGAVN IKEQG+CGSCWAFS+VAAVEGIN+IKT QLLSLSEQ
Sbjct: 148 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSSVAAVEGINQIKTNQLLSLSEQ 207

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATENNYPYHGARG CRSSR     +TIDGY
Sbjct: 208 ELLDCNFRNKGCNGGFMEIAFDFIKKNGGIATENNYPYHGARGLCRSSRKSSPTVTIDGY 267

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYS-------------------------- 300
           E+VP NENALMQAVANQPVSVAIDA G+DFQFYS                          
Sbjct: 268 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLWLIRAIDCFTIISSGIVNLKTV 327

Query: 301 ---EGVFDGECGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDPE 343
              +GVFDG CGTELNHGVVAIGYGTTE+GTDYW+VRNSWGVGWGEEGYVRMK+GVE  E
Sbjct: 328 GKLQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEQSE 387

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BLAST of Spg034147 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 4.2e-128
Identity = 220/347 (63.40%), Postives = 275/347 (79.25%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MA+ K L V +L + LV G+A SF+F EK+L +EESLW LYERW  HHT+SR L EKHKR
Sbjct: 1   MAMKKLLWV-VLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F+VFK NV HV   N+M+KPYKLKLNKFADM+N+EF + YA S ++H++   G + G  G
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGS-G 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
            FMYE+V  +PA++DWR++GAV  +K+QG+CGSCWAFST+ AVEGIN+IKT +L+SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 181 ELLDCN-TRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRV----ITIDG 240
           EL+DC+   N GCNGG ME AF+FI+Q GGI TE+NYPY    G C  S+V    ++IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDG 240

Query: 241 YENVPIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTT 300
           +ENVP+N ENAL++AVANQPVSVAIDA G DFQFYSEGVF G+C T+LNHGV  +GYGTT
Sbjct: 241 HENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTT 300

Query: 301 EDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
            DGT+YW+VRNSWG  WGE+GY+RM++ +   EG+CGIAM ASYPIK
Sbjct: 301 VDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

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BLAST of Spg034147 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 2.7e-127
Identity = 220/343 (64.14%), Postives = 269/343 (78.43%), Query Frame = 0


Query: 5   KFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVF 64
           KF+L+ L L  LV  + +SF+F EKEL +EESLW LYERW  HHT+SR L EK KRF+VF
Sbjct: 3   KFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNVF 62

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMY 124
           K N  HV   N+M+KPYKLKLNKFADM+N+EF N Y+ S + H+R   G  RG  G FMY
Sbjct: 63  KHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTFMY 122

Query: 125 EQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLD 184
           E+V  +PA++DWR++GAV  +K+QG+CGSCWAFST+ AVEGIN+IKT +L+SLSEQEL+D
Sbjct: 123 EKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVD 182

Query: 185 CNT-RNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGYENV 244
           C+T +N GCNGG M+ AF+FI+Q GGI TE NYPY    G C  S+     ++IDG+ENV
Sbjct: 183 CDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHENV 242

Query: 245 PIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGT 304
           P N ENAL++AVANQPVSVAIDA G DFQFYSEGVF G CGTEL+HGV  +GYGTT DGT
Sbjct: 243 PENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTIDGT 302

Query: 305 DYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
            YW V+NSWG  WGE+GY+RM++G+ D EG+CGIAMEASYPIK
Sbjct: 303 KYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

[Close]

BLAST of Spg034147 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 3.7e-124
Identity = 214/343 (62.39%), Postives = 266/343 (77.55%), Query Frame = 0


Query: 5   KFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVF 64
           K LL  +L   LV G+A SF+F +K+LA+EESLW LYERW  HHT+SR L EKHKRF+VF
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMY 124
           K N+ HV   N+M+KPYKLKLNKFADM+N+EF + YA S ++H R   G      G FMY
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHEN-GAFMY 123

Query: 125 EQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLD 184
           E+V  +P ++DWR++GAV  +K+QG+CGSCWAFSTV AVEGIN+IKT +L++LSEQEL+D
Sbjct: 124 EKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVD 183

Query: 185 CN-TRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRV----ITIDGYENV 244
           C+   N GCNGG ME AF+FI+Q GGI TE+NYPY    G C +S+V    ++IDG+ENV
Sbjct: 184 CDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENV 243

Query: 245 PIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGT 304
           P N E+AL++AVANQPVSVAIDA G DFQFYSEGVF G+C T+LNHGV  +GYGTT DGT
Sbjct: 244 PANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTVDGT 303

Query: 305 DYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
           +YW+VRNSWG  WGE GY+RM++ +   EG+CGIAM  SYPIK
Sbjct: 304 NYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

[Close]

BLAST of Spg034147 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.6e-119
Identity = 212/343 (61.81%), Postives = 266/343 (77.55%), Query Frame = 0


Query: 8   LVFLLLIVLVSGL--AKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVFK 67
           L F++LI  +S L  +K F+FDEKEL TEE++WKLYERW  HH++SR   E  KRF+VF+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  ENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMYE 127
            NV HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G KRG  GGFMYE
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYE 122

Query: 128 QVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLDC 187
            VT +P+++DWRE+GAV  +K Q  CGSCWAFSTVAAVEGINKI+T +L+SLSEQEL+DC
Sbjct: 123 NVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDC 182

Query: 188 NT-RNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGAR-GFCRSSRV----ITIDGYENV 247
           +T  N GC GG ME AF+FI+ NGGI TE  YPY  +   FCR++ +    +TIDG+E+V
Sbjct: 183 DTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHV 242

Query: 248 PIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGT 307
           P N E  L++AVA+QPVSVAIDA   DFQ YSEGVF GECGT+LNHGVV +GYG T++GT
Sbjct: 243 PENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGT 302

Query: 308 DYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
            YW+VRNSWG  WGE GYVR+++G+ + EG CGIAMEASYP K
Sbjct: 303 KYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of Spg034147 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.0e-118
Identity = 211/345 (61.16%), Postives = 268/345 (77.68%), Query Frame = 0


Query: 5   KFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVF 64
           K LL+FL  +V++   A  F++D+KE+ +EE L  LY+RW  HH++ R L E+ KRF+VF
Sbjct: 3   KLLLIFLFSLVILQ-TACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 62

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMY 124
           + NV HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G KRG    FMY
Sbjct: 63  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGS-KQFMY 122

Query: 125 --EQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQEL 184
             E ++ LP+++DWR++GAV  IK QG+CGSCWAFSTVAAVEGINKIKT +L+SLSEQEL
Sbjct: 123 DHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQEL 182

Query: 185 LDCNTR-NSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGYE 244
           +DC+T+ N GCNGG MEIAF+FI++NGGI TE++YPY G  G C +S+    ++TIDG+E
Sbjct: 183 VDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHE 242

Query: 245 NVPIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 304
           +VP N ENAL++AVANQPVSVAIDA   DFQFYSEGVF G CGTELNHGV A+GYG +E 
Sbjct: 243 DVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SER 302

Query: 305 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
           G  YW+VRNSWG  WGE GY+++++ +++PEG CGIAMEASYPIK
Sbjct: 303 GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of Spg034147 vs. ExPASy TrEMBL
Match: A0A1S3BA70 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.9e-173
Identity = 298/346 (86.13%), Postives = 322/346 (93.06%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLLV LLLIVLVSGLA+SFEFDEKELATEESLW+LYERW +HHTISR+LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F VFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVN YARSNISH+RKLHGR+R G G
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+++DWRERGAVN IKEQG CGSCWAFS+VAAVE INKIKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATEN+YPYHG+RG CRSSR    ++ IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NE+ALMQAVANQPVSVAIDAAG+DFQFY +GVFDG CGTELNHGVVAIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYW+VRNSWGVGWGE+GYVRMK+GVE PEG+CGIAMEASYPIKF
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

[Close]

BLAST of Spg034147 vs. ExPASy TrEMBL
Match: A0A0A0LMU4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.0e-172
Identity = 298/346 (86.13%), Postives = 322/346 (93.06%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KFLLV LLLIVLVSGLA+SFEFDEKELATEESLW+LYERW  HHTISR+LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F VFKENVNHVFTVNQM+KPYKLKLNKFADMSNYEFVN YARSNISHYRKLH R+R G G
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRR-GAG 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+++DWRERGAVN +KEQGRCGSCWAFS+VAAVEGINKIKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATEN+YPYHG+RG CRSSR    ++ IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NE+ALMQAVANQPVSVAIDAAG+DFQFYS+GVFDG CGTELNHGVVAIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYWLVRNSWGVGWGE+GYVRMK+GVE  EG+CGIAMEASYPIK+
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

[Close]

BLAST of Spg034147 vs. ExPASy TrEMBL
Match: A0A6J1K7P4 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.5e-165
Identity = 294/376 (78.19%), Postives = 317/376 (84.31%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI KF+LV  LLIVLVSGL +SFEFDEKELATEESLW+LYERW +HHTISR LKEKHKR
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           F+VFKENVNHVFTVNQMNKPYKLKLNKFADMSN EFV+ YARSNISHYRKLHG++    G
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKRS---G 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYEQ TDLP+ IDWRERGAVN IKEQG+CGSCWAFSTVAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRV----ITIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI++NGGIATENNYPYHGARG CRSSR+    +TIDGY
Sbjct: 181 ELLDCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGY 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYS-------------------------- 300
           E+VP NENALMQAVANQPVSVAIDA G+DFQFYS                          
Sbjct: 241 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKS 300

Query: 301 ----EGVFDGECGTELNHGVVAIGYGTTEDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDP 343
               +GVFDG CGTELNHGVVAIGYGTTE+GTDYW+VRNSWGVGWGEEGYVRMK+GVE  
Sbjct: 301 VGKVQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKA 360

[Close]

BLAST of Spg034147 vs. ExPASy TrEMBL
Match: A0A6J1KIL0 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.0e-164
Identity = 282/346 (81.50%), Postives = 311/346 (89.88%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           M I K LLV   LIVLVSGLAKSFEFDE+ELAT+ SLWKLYERWSHHH ISR+LKEKHKR
Sbjct: 1   MPISKCLLV-PFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           ++VFKEN NHV TVNQMNKPYKLKLNKFADMSNYEFVNLYARSNI+HYR+LHGR+R G  
Sbjct: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYE+ TDLP+ IDWRERGAVN IK QGRCGSCWAFS VAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCNTRN GC GGFME A++FIR+NGGIA+ENNYPY GARG CRSSR    ++TIDG+
Sbjct: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NENALMQAVANQPVSV+I+A G+DFQFY +GVFDG CGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYW VRNSWGVGWGE+GY+RMK+GVEDPEG+CGIAMEASYP+KF
Sbjct: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 345

[Close]

BLAST of Spg034147 vs. ExPASy TrEMBL
Match: A0A6J1GHN5 (vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.1e-163
Identity = 278/346 (80.35%), Postives = 313/346 (90.46%), Query Frame = 0


Query: 1   MAIPKFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKR 60
           MAI K LLV   LIVL+SGLAKSFEFDE+ELAT+ SLWKLYERWSHHH ISR+LKEKHKR
Sbjct: 1   MAISKCLLV-PFLIVLISGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60

Query: 61  FDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVG 120
           ++VFKEN NHV TVNQMNKPYKLKLNKFADMSNYEFVN+YARSNI+HYR+LHG++R G  
Sbjct: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRREGAS 120

Query: 121 GFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQ 180
           GFMYE+ TDLP+ IDWRERGAV+ IK+QGRCGSCWAFS VAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGY 240
           ELLDCNTRN GC GGFME A++FIR+NGGIA+ENNYPY GARG CRSSR    ++TIDG+
Sbjct: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240

Query: 241 ENVPINENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 300
           E+VP NENALMQAVANQPVSV+I+A G+DFQFY +GVFDG+CGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDG 300

Query: 301 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIKF 343
           GTDYW VRNSWGVGWGE+GY+RMK+GVEDPEG+CGI MEASYP+KF
Sbjct: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

[Close]

BLAST of Spg034147 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.6e-120
Identity = 212/343 (61.81%), Postives = 266/343 (77.55%), Query Frame = 0


Query: 8   LVFLLLIVLVSGL--AKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVFK 67
           L F++LI  +S L  +K F+FDEKEL TEE++WKLYERW  HH++SR   E  KRF+VF+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  ENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMYE 127
            NV HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G KRG  GGFMYE
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGS-GGFMYE 122

Query: 128 QVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLDC 187
            VT +P+++DWRE+GAV  +K Q  CGSCWAFSTVAAVEGINKI+T +L+SLSEQEL+DC
Sbjct: 123 NVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDC 182

Query: 188 NT-RNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGAR-GFCRSSRV----ITIDGYENV 247
           +T  N GC GG ME AF+FI+ NGGI TE  YPY  +   FCR++ +    +TIDG+E+V
Sbjct: 183 DTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHV 242

Query: 248 PIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGT 307
           P N E  L++AVA+QPVSVAIDA   DFQ YSEGVF GECGT+LNHGVV +GYG T++GT
Sbjct: 243 PENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGT 302

Query: 308 DYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
            YW+VRNSWG  WGE GYVR+++G+ + EG CGIAMEASYP K
Sbjct: 303 KYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of Spg034147 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 427.9 bits (1099), Expect = 7.4e-120
Identity = 211/345 (61.16%), Postives = 268/345 (77.68%), Query Frame = 0


Query: 5   KFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVF 64
           K LL+FL  +V++   A  F++D+KE+ +EE L  LY+RW  HH++ R L E+ KRF+VF
Sbjct: 3   KLLLIFLFSLVILQ-TACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNVF 62

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMY 124
           + NV HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G KRG    FMY
Sbjct: 63  RHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGS-KQFMY 122

Query: 125 --EQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQEL 184
             E ++ LP+++DWR++GAV  IK QG+CGSCWAFSTVAAVEGINKIKT +L+SLSEQEL
Sbjct: 123 DHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQEL 182

Query: 185 LDCNTR-NSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGYE 244
           +DC+T+ N GCNGG MEIAF+FI++NGGI TE++YPY G  G C +S+    ++TIDG+E
Sbjct: 183 VDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGHE 242

Query: 245 NVPIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTED 304
           +VP N ENAL++AVANQPVSVAIDA   DFQFYSEGVF G CGTELNHGV A+GYG +E 
Sbjct: 243 DVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SER 302

Query: 305 GTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
           G  YW+VRNSWG  WGE GY+++++ +++PEG CGIAMEASYPIK
Sbjct: 303 GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of Spg034147 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 419.5 bits (1077), Expect = 2.6e-117
Identity = 199/343 (58.02%), Postives = 257/343 (74.93%), Query Frame = 0


Query: 5   KFLLVFLLLIVLVSGLAKSFEFDEKELATEESLWKLYERWSHHHTISRDLKEKHKRFDVF 64
           K  +V  L +++V    K  +F  K++ +E SLW+LYERW  HHT++R L+EK KRF+VF
Sbjct: 2   KRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNVF 61

Query: 65  KENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMY 124
           K NV H+   N+ +K YKLKLNKF DM++ EF   YA SNI H+R   G K+     FMY
Sbjct: 62  KHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKK-ATKSFMY 121

Query: 125 EQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLD 184
             V  LP ++DWR+ GAV  +K QG+CGSCWAFSTV AVEGIN+I+TK+L SLSEQEL+D
Sbjct: 122 ANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVD 181

Query: 185 CNT-RNSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSR----VITIDGYENV 244
           C+T +N GCNGG M++AF+FI++ GG+ +E  YPY  +   C +++    V++IDG+E+V
Sbjct: 182 CDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDV 241

Query: 245 PIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGT 304
           P N E+ LM+AVANQPVSVAIDA G DFQFYSEGVF G CGTELNHGV  +GYGTT DGT
Sbjct: 242 PKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGT 301

Query: 305 DYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
            YW+V+NSWG  WGE+GY+RM++G+   EG+CGIAMEASYP+K
Sbjct: 302 KYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

[Close]

BLAST of Spg034147 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.4e-88
Identity = 172/354 (48.59%), Postives = 236/354 (66.67%), Query Frame = 0


Query: 11  LLLIVLVSGLAKSFE-----FDEKELATEES------LWKLYERWSHHHTISRDLK---- 70
           ++L++ + G++ + +     +DE    T E+      + ++YE W   H   +  +    
Sbjct: 9   MILLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLG 68

Query: 71  -EKHKRFDVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSN-ISHYRKLHG 130
            EK +RF++FK+N+  +   N  N  YKL L +FAD++N E+ ++Y  +       K   
Sbjct: 69  AEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAKPTKRVLKTSD 128

Query: 131 RKRGGVGGFMYEQVTDLPAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQ 190
           R +  VG         LP ++DWR+ GAV  +K+QG CGSCWAFST+ AVEGINKI T  
Sbjct: 129 RYQARVG-------DALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGD 188

Query: 191 LLSLSEQELLDCNTR-NSGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFC----RSS 250
           L+SLSEQEL+DC+T  N GCNGG M+ AF+FI +NGGI TE +YPY  A G C    +++
Sbjct: 189 LISLSEQELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNA 248

Query: 251 RVITIDGYENVPIN-ENALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVV 310
           +V+TID YE+VP N E +L +A+A+QP+SVAI+A G+ FQ YS GVFDG CGTEL+HGVV
Sbjct: 249 KVVTIDSYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVV 308

Query: 311 AIGYGTTEDGTDYWLVRNSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
           A+GYG TE+G DYW+VRNSWG  WGE GY++M + +E P G CGIAMEASYPIK
Sbjct: 309 AVGYG-TENGKDYWIVRNSWGNRWGESGYIKMARNIEAPTGKCGIAMEASYPIK 354

[Close]

BLAST of Spg034147 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 321.6 bits (823), Expect = 7.5e-88
Identity = 163/337 (48.37%), Postives = 229/337 (67.95%), Query Frame = 0


Query: 15  VLVSGLAKSFE---FDEKELATEESLWKLYERW-SHHHTISRDLKEKHKRFDVFKENVNH 74
           +L    A+ F    +  + L   + L +L+E W S H    + ++EK  RF+VF+EN+ H
Sbjct: 22  LLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMH 81

Query: 75  VFTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNISHYRKLHGRKRGGVGGFMYEQVTDL 134
           +   N     Y L LN+FAD+++ EF   Y    +   +    RKR     F Y  +TDL
Sbjct: 82  IDQRNNEINSYWLGLNEFADLTHEEFKGRY----LGLAKPQFSRKRQPSANFRYRDITDL 141

Query: 135 PAAIDWRERGAVNGIKEQGRCGSCWAFSTVAAVEGINKIKTKQLLSLSEQELLDCNTR-N 194
           P ++DWR++GAV  +K+QG+CGSCWAFSTVAAVEGIN+I T  L SLSEQEL+DC+T  N
Sbjct: 142 PKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFN 201

Query: 195 SGCNGGFMEIAFDFIRQNGGIATENNYPYHGARGFCRSSRV----ITIDGYENVPINEN- 254
           SGCNGG M+ AF +I   GG+  E++YPY    G C+  +     +TI GYE+VP N++ 
Sbjct: 202 SGCNGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDE 261

Query: 255 ALMQAVANQPVSVAIDAAGKDFQFYSEGVFDGECGTELNHGVVAIGYGTTEDGTDYWLVR 314
           +L++A+A+QPVSVAI+A+G+DFQFY  GVF+G+CGT+L+HGV A+GYG+++ G+DY +V+
Sbjct: 262 SLVKALAHQPVSVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSK-GSDYVIVK 321

Query: 315 NSWGVGWGEEGYVRMKKGVEDPEGMCGIAMEASYPIK 342
           NSWG  WGE+G++RMK+    PEG+CGI   ASYP K
Sbjct: 322 NSWGPRWGEKGFIRMKRNTGKPEGLCGINKMASYPTK 353

The following BLAST results are available for this feature:

BLAST of Spg034147 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885064.1	2.0e-175	86.71	LOW QUALITY PROTEIN: vignain-like [Benincasa hispida]	[more]
XP_008444390.1	1.2e-172	86.13	PREDICTED: vignain-like [Cucumis melo]	[more]
XP_004142960.1	2.1e-172	86.13	vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis s...	[more]
XP_022996765.1	3.2e-165	78.19	vignain-like [Cucurbita maxima]	[more]
XP_023545499.1	7.1e-165	78.40	vignain-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg034147 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P12412	4.2e-128	63.40	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
O65039	2.7e-127	64.14	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P25803	3.7e-124	62.39	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL5	3.6e-119	61.81	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]
Q9STL4	1.0e-118	61.16	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]

BLAST of Spg034147 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BA70	5.9e-173	86.13	vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1	[more]
A0A0A0LMU4	1.0e-172	86.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1	[more]
A0A6J1K7P4	1.5e-165	78.19	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1	[more]
A0A6J1KIL0	5.0e-164	81.50	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1	[more]
A0A6J1GHN5	1.1e-163	80.35	vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1	[more]

BLAST of Spg034147 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48350.1	2.6e-120	61.81	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	7.4e-120	61.16	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	2.6e-117	58.02	Cysteine proteinases superfamily protein	[more]
AT5G43060.1	3.4e-88	48.59	Granulin repeat cysteine protease family protein	[more]
AT4G35350.1	7.5e-88	48.37	xylem cysteine peptidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 148..163 score: 65.4 coord: 284..294 score: 50.75 coord: 300..306 score: 69.74
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 130..340 e-value: 5.9E-116 score: 401.2
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 130..340 e-value: 1.1E-76 score: 257.8
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..96 e-value: 1.1E-9 score: 48.2
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..96 e-value: 4.0E-11 score: 43.2
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 16..342 e-value: 6.4E-117 score: 392.9
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 21..341
None	No IPR available	PANTHER	PTHR12411:SF706	KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1	coord: 21..341
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 300..319
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 148..159
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 282..292
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 131..339 e-value: 8.79655E-91 score: 268.723
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 33..340

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034147.1	Spg034147.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity