Spg033529 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033529 vs. NCBI nr
Match: KAG7017258.1 (hypothetical protein SDJN02_19121 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.8 bits (1514), Expect = 6.8e-164
Identity = 304/388 (78.35%), Postives = 330/388 (85.05%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADDDDQKS---CVGNKNTDQNRLGSTTFQSGRASFPLEFGTFG 60
           MADSLDDGEFWLPPQFLADDD+  S   C  NKN+D N L ST F   R SFP EFGTFG
Sbjct: 1   MADSLDDGEFWLPPQFLADDDNMPSPNTCATNKNSDLNCLASTPFLPRRPSFPFEFGTFG 60

Query: 61  GFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTL 120
           GFSDFSSPGESLKGSSETESDEED +AG+TLRMA+STIDDGFD+ N K  + +GSPQSTL
Sbjct: 61  GFSDFSSPGESLKGSSETESDEEDCLAGITLRMAQSTIDDGFDSYNSKTSLLSGSPQSTL 120

Query: 121 CDMGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAP 180
           CDMGSGSGCSQ SSRGSPK N KVPSPPAT DLLHAAAGEVARMRMNES GV+  NRG  
Sbjct: 121 CDMGSGSGCSQVSSRGSPKSNCKVPSPPATCDLLHAAAGEVARMRMNESRGVISQNRGTS 180

Query: 181 QVSVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMV 240
           QVSVPV NSSTGTGFYQQLQAMQ  HL QKE+MQRQN  VGEQ+NSPAGYQ+ QQ+H MV
Sbjct: 181 QVSVPVKNSSTGTGFYQQLQAMQVTHLNQKEIMQRQNFTVGEQMNSPAGYQR-QQVHVMV 240

Query: 241 QNGMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPV 300
           QNG+RG RGLSSS+W+PPPQGSG RALFLG  G KRECAGTGVFLPRH+  QSE R+KP 
Sbjct: 241 QNGVRGCRGLSSSAWIPPPQGSGTRALFLGTQGSKRECAGTGVFLPRHSSTQSEQRRKP- 300

Query: 301 KANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQIN 360
            ANHEF+   TACSTVLVPARVMQALNLNL+DICSQPH++P+ GGR NSENDVLLRLQIN
Sbjct: 301 -ANHEFVVLLTACSTVLVPARVMQALNLNLEDICSQPHVEPMGGGRLNSENDVLLRLQIN 360

Query: 361 RGAHHQKRNSRRQPPTDHEIKLPQEWTY 386
           RG +++KRN R Q PTDHEIKLPQEWTY
Sbjct: 361 RGGNYEKRNGRGQLPTDHEIKLPQEWTY 385

BLAST of Spg033529 vs. NCBI nr
Match: XP_022971330.1 (uncharacterized protein LOC111470084 [Cucurbita maxima])

HSP 1 Score: 585.1 bits (1507), Expect = 4.4e-163
Identity = 303/386 (78.50%), Postives = 325/386 (84.20%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADD-DDQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFGGF 60
           MA+SLDDGEFWLPPQFL+DD DDQ+SC  NKN  +N L ST FQSGR SFPLEFGTFGGF
Sbjct: 1   MAESLDDGEFWLPPQFLSDDHDDQESCATNKNNGRNSLCSTAFQSGRPSFPLEFGTFGGF 60

Query: 61  SDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCD 120
           SDFSS  ESLKGSSETESD ED VAGLTL MARST+DDG D+DN K  V AGSPQSTLCD
Sbjct: 61  SDFSSTTESLKGSSETESDLEDLVAGLTLHMARSTVDDGLDSDNAKEIVLAGSPQSTLCD 120

Query: 121 MGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQV 180
           MGSGSGCSQGSSRGSPKGN KVPSPPATWDLLHAAAG+VARMRM E+HGVVH NRGAPQV
Sbjct: 121 MGSGSGCSQGSSRGSPKGNCKVPSPPATWDLLHAAAGQVARMRMTENHGVVHQNRGAPQV 180

Query: 181 SVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQN 240
           S+PV NSS+  GFYQQLQAMQFQHLQQKE+MQRQN+ V EQIN  AGYQQ QQIHQ+V N
Sbjct: 181 SLPVKNSSSDNGFYQQLQAMQFQHLQQKEIMQRQNLTVAEQINYHAGYQQ-QQIHQIVHN 240

Query: 241 GMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPVKA 300
           GM   RGLSSS W  PPQGSG R+LF+G PGGKRECAGTGVFLPRHT   S+ R+KP   
Sbjct: 241 GMMSGRGLSSSPWRQPPQGSGTRSLFVGTPGGKRECAGTGVFLPRHTATHSQQRRKP--- 300

Query: 301 NHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQINRG 360
                     C TVLVPARVMQALNLNL+DICSQPHLQPVA GRFNS+NDVLLRLQ NRG
Sbjct: 301 ---------DCPTVLVPARVMQALNLNLEDICSQPHLQPVAAGRFNSDNDVLLRLQFNRG 360

Query: 361 AHHQKRNSRRQPPTDHEIKLPQEWTY 386
           A++QKRNSRRQ  T+HEIKLPQEWTY
Sbjct: 361 ANYQKRNSRRQAATEHEIKLPQEWTY 373

BLAST of Spg033529 vs. NCBI nr
Match: XP_023540064.1 (uncharacterized protein LOC111800546 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.3e-162
Identity = 302/386 (78.24%), Postives = 324/386 (83.94%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLA-DDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFGGF 60
           MA+SLDDGEFWLPPQFL+ DDDDQ+S   NKN  +N  GST FQSGR SFPLEFGTFGGF
Sbjct: 1   MAESLDDGEFWLPPQFLSDDDDDQESSATNKNNGRNSFGSTAFQSGRPSFPLEFGTFGGF 60

Query: 61  SDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCD 120
           SDFSS  ESLKGSSETESD ED VAGLTL MARST+DDG D+DN K RV AGSPQSTLCD
Sbjct: 61  SDFSSTAESLKGSSETESDLEDIVAGLTLHMARSTVDDGLDSDNAKERVLAGSPQSTLCD 120

Query: 121 MGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQV 180
           MGSGSGCSQGSSRGSPK N KVPSPPATWDLLHAAAG+VARMRM E+HGVVH NRG PQV
Sbjct: 121 MGSGSGCSQGSSRGSPKSNCKVPSPPATWDLLHAAAGQVARMRMTENHGVVHQNRGTPQV 180

Query: 181 SVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQN 240
           SVPV NSS+ TGFYQQLQAMQFQHLQQKE+MQRQN+ V EQIN  AGYQQ QQIHQ+V N
Sbjct: 181 SVPVKNSSSDTGFYQQLQAMQFQHLQQKEIMQRQNLTVAEQINYHAGYQQ-QQIHQIVHN 240

Query: 241 GMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPVKA 300
           GMR  RGLSSS W  PPQG+G RALF+G PGGKRECAGTGVFLPRHT   S+ R+KP   
Sbjct: 241 GMRSGRGLSSSPWRQPPQGAGARALFVGTPGGKRECAGTGVFLPRHTATHSQQRRKP--- 300

Query: 301 NHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQINRG 360
                     C TVLVPARVMQALNLNL+DICSQPHLQPVA  RFNS+NDVLLRLQ NRG
Sbjct: 301 ---------DCPTVLVPARVMQALNLNLEDICSQPHLQPVAAARFNSDNDVLLRLQFNRG 360

Query: 361 AHHQKRNSRRQPPTDHEIKLPQEWTY 386
           A++QKRNSRRQ  T++EIKLPQEWTY
Sbjct: 361 ANYQKRNSRRQAATENEIKLPQEWTY 373

BLAST of Spg033529 vs. NCBI nr
Match: XP_022145688.1 (uncharacterized protein LOC111015081 [Momordica charantia])

HSP 1 Score: 581.3 bits (1497), Expect = 6.4e-162
Identity = 313/403 (77.67%), Postives = 335/403 (83.13%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADDD---DQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFG 60
           MA+SLDDGEFWLPPQFLADDD   D KSC   K++D+N L     Q GRASFPLEFGTFG
Sbjct: 2   MAESLDDGEFWLPPQFLADDDNMLDHKSCNTRKSSDRNCLS----QFGRASFPLEFGTFG 61

Query: 61  GFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTL 120
           GFSDFSSPGESLKGSSETESDEEDY+AGLTLRMA S++DD FD DN KGRV +GSPQSTL
Sbjct: 62  GFSDFSSPGESLKGSSETESDEEDYMAGLTLRMAHSSVDDAFDPDNAKGRVVSGSPQSTL 121

Query: 121 CDMGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNE-SHGVVHNNRGA 180
           C MGSGSGCSQGSSRGSPKGN KV SPPATWDLLHAAAGEVARMR N+ +HGV H+NRG 
Sbjct: 122 CGMGSGSGCSQGSSRGSPKGNCKVSSPPATWDLLHAAAGEVARMRTNDCAHGVHHHNRGT 181

Query: 181 PQVSVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQM 240
           PQVSVPVNNS+ GTGFYQQL AMQF    Q+E+MQRQN+A GEQ+NS AGYQQ QQIHQM
Sbjct: 182 PQVSVPVNNSNAGTGFYQQLHAMQF----QQEIMQRQNLAAGEQMNSHAGYQQ-QQIHQM 241

Query: 241 VQNGMRG----------NRGLSSSSWLP---PPQGSGMRALFLG-PPGGKRECAGTGVFL 300
           V NG+RG          +RGLSSS+WLP   PPQGSGMRALFLG PP GKRECAGTGVFL
Sbjct: 242 VHNGVRGGEFVGNRDHRSRGLSSSAWLPPQQPPQGSGMRALFLGTPPAGKRECAGTGVFL 301

Query: 301 PRHTGAQSEPRKKPVKANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGG 360
           PRHTG QSEPRKKP             CSTVLVPARVMQALNLNLDDICSQPHLQPVA G
Sbjct: 302 PRHTGTQSEPRKKP------------GCSTVLVPARVMQALNLNLDDICSQPHLQPVAAG 361

Query: 361 RFNSENDVLLRLQINRGAHHQKRNSRRQPPTDHEIKLPQEWTY 386
           RFN+ENDVLLRLQINRGA+ QKRNSRRQPPTD E+KLPQEWTY
Sbjct: 362 RFNAENDVLLRLQINRGANQQKRNSRRQPPTDRELKLPQEWTY 383

BLAST of Spg033529 vs. NCBI nr
Match: XP_022941783.1 (uncharacterized protein LOC111447045 [Cucurbita moschata] >KAG6596379.1 hypothetical protein SDJN03_09559, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.5 bits (1495), Expect = 1.1e-161
Identity = 304/387 (78.55%), Postives = 324/387 (83.72%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLA-DDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFGGF 60
           MA+SLDDGEFWLPPQFL+ DDDDQ+S   NKN  +N  GST FQSGR SFPLEFGTFGGF
Sbjct: 1   MAESLDDGEFWLPPQFLSDDDDDQESSATNKNNGRNSFGSTAFQSGRPSFPLEFGTFGGF 60

Query: 61  SDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCD 120
           SDFSS  ESLKGSSETESD ED VAGLTL MARST+DDG D+DN K RV AGSPQSTLCD
Sbjct: 61  SDFSSTAESLKGSSETESDLEDLVAGLTLHMARSTVDDGLDSDNAKERVLAGSPQSTLCD 120

Query: 121 MGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQV 180
           MGSGSGCSQGSSRGSPKGN KVPSPPATWDLLHAAAG+VARMRM E+HGVVH NRGA QV
Sbjct: 121 MGSGSGCSQGSSRGSPKGNCKVPSPPATWDLLHAAAGQVARMRMTENHGVVHQNRGASQV 180

Query: 181 SVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQN 240
           SVPV NSS+ TGFYQQLQAMQFQHLQQKE+MQRQN  V EQIN  AGYQQ QQIHQ+VQN
Sbjct: 181 SVPVKNSSSDTGFYQQLQAMQFQHLQQKEIMQRQNPTVAEQINYHAGYQQ-QQIHQIVQN 240

Query: 241 GMRGNRGLSSSSWLPPPQGSGMRALFLGPP-GGKRECAGTGVFLPRHTGAQSEPRKKPVK 300
           GMR  RGLSSS W  PPQG+G RALF+G   GGKRECAGTGVFLPRHT   S+PR+KP  
Sbjct: 241 GMRSGRGLSSSPWRQPPQGAGTRALFVGTSGGGKRECAGTGVFLPRHTATHSQPRRKP-- 300

Query: 301 ANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQINR 360
                      C TVLVPARVMQALNLNL+DICSQPHLQPVA  RFNS+NDVLLRLQ NR
Sbjct: 301 ----------DCPTVLVPARVMQALNLNLEDICSQPHLQPVAAARFNSDNDVLLRLQFNR 360

Query: 361 GAHHQKRNSRRQPPTDHEIKLPQEWTY 386
            A++QKRNSRRQ  T+HEIKLPQEWTY
Sbjct: 361 SANYQKRNSRRQAATEHEIKLPQEWTY 374

BLAST of Spg033529 vs. ExPASy TrEMBL
Match: A0A6J1I5F7 (uncharacterized protein LOC111470084 OS=Cucurbita maxima OX=3661 GN=LOC111470084 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.1e-163
Identity = 303/386 (78.50%), Postives = 325/386 (84.20%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADD-DDQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFGGF 60
           MA+SLDDGEFWLPPQFL+DD DDQ+SC  NKN  +N L ST FQSGR SFPLEFGTFGGF
Sbjct: 1   MAESLDDGEFWLPPQFLSDDHDDQESCATNKNNGRNSLCSTAFQSGRPSFPLEFGTFGGF 60

Query: 61  SDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCD 120
           SDFSS  ESLKGSSETESD ED VAGLTL MARST+DDG D+DN K  V AGSPQSTLCD
Sbjct: 61  SDFSSTTESLKGSSETESDLEDLVAGLTLHMARSTVDDGLDSDNAKEIVLAGSPQSTLCD 120

Query: 121 MGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQV 180
           MGSGSGCSQGSSRGSPKGN KVPSPPATWDLLHAAAG+VARMRM E+HGVVH NRGAPQV
Sbjct: 121 MGSGSGCSQGSSRGSPKGNCKVPSPPATWDLLHAAAGQVARMRMTENHGVVHQNRGAPQV 180

Query: 181 SVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQN 240
           S+PV NSS+  GFYQQLQAMQFQHLQQKE+MQRQN+ V EQIN  AGYQQ QQIHQ+V N
Sbjct: 181 SLPVKNSSSDNGFYQQLQAMQFQHLQQKEIMQRQNLTVAEQINYHAGYQQ-QQIHQIVHN 240

Query: 241 GMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPVKA 300
           GM   RGLSSS W  PPQGSG R+LF+G PGGKRECAGTGVFLPRHT   S+ R+KP   
Sbjct: 241 GMMSGRGLSSSPWRQPPQGSGTRSLFVGTPGGKRECAGTGVFLPRHTATHSQQRRKP--- 300

Query: 301 NHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQINRG 360
                     C TVLVPARVMQALNLNL+DICSQPHLQPVA GRFNS+NDVLLRLQ NRG
Sbjct: 301 ---------DCPTVLVPARVMQALNLNLEDICSQPHLQPVAAGRFNSDNDVLLRLQFNRG 360

Query: 361 AHHQKRNSRRQPPTDHEIKLPQEWTY 386
           A++QKRNSRRQ  T+HEIKLPQEWTY
Sbjct: 361 ANYQKRNSRRQAATEHEIKLPQEWTY 373

BLAST of Spg033529 vs. ExPASy TrEMBL
Match: A0A6J1CWM5 (uncharacterized protein LOC111015081 OS=Momordica charantia OX=3673 GN=LOC111015081 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 3.1e-162
Identity = 313/403 (77.67%), Postives = 335/403 (83.13%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADDD---DQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFG 60
           MA+SLDDGEFWLPPQFLADDD   D KSC   K++D+N L     Q GRASFPLEFGTFG
Sbjct: 2   MAESLDDGEFWLPPQFLADDDNMLDHKSCNTRKSSDRNCLS----QFGRASFPLEFGTFG 61

Query: 61  GFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTL 120
           GFSDFSSPGESLKGSSETESDEEDY+AGLTLRMA S++DD FD DN KGRV +GSPQSTL
Sbjct: 62  GFSDFSSPGESLKGSSETESDEEDYMAGLTLRMAHSSVDDAFDPDNAKGRVVSGSPQSTL 121

Query: 121 CDMGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNE-SHGVVHNNRGA 180
           C MGSGSGCSQGSSRGSPKGN KV SPPATWDLLHAAAGEVARMR N+ +HGV H+NRG 
Sbjct: 122 CGMGSGSGCSQGSSRGSPKGNCKVSSPPATWDLLHAAAGEVARMRTNDCAHGVHHHNRGT 181

Query: 181 PQVSVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQM 240
           PQVSVPVNNS+ GTGFYQQL AMQF    Q+E+MQRQN+A GEQ+NS AGYQQ QQIHQM
Sbjct: 182 PQVSVPVNNSNAGTGFYQQLHAMQF----QQEIMQRQNLAAGEQMNSHAGYQQ-QQIHQM 241

Query: 241 VQNGMRG----------NRGLSSSSWLP---PPQGSGMRALFLG-PPGGKRECAGTGVFL 300
           V NG+RG          +RGLSSS+WLP   PPQGSGMRALFLG PP GKRECAGTGVFL
Sbjct: 242 VHNGVRGGEFVGNRDHRSRGLSSSAWLPPQQPPQGSGMRALFLGTPPAGKRECAGTGVFL 301

Query: 301 PRHTGAQSEPRKKPVKANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGG 360
           PRHTG QSEPRKKP             CSTVLVPARVMQALNLNLDDICSQPHLQPVA G
Sbjct: 302 PRHTGTQSEPRKKP------------GCSTVLVPARVMQALNLNLDDICSQPHLQPVAAG 361

Query: 361 RFNSENDVLLRLQINRGAHHQKRNSRRQPPTDHEIKLPQEWTY 386
           RFN+ENDVLLRLQINRGA+ QKRNSRRQPPTD E+KLPQEWTY
Sbjct: 362 RFNAENDVLLRLQINRGANQQKRNSRRQPPTDRELKLPQEWTY 383

BLAST of Spg033529 vs. ExPASy TrEMBL
Match: A0A6J1FT27 (uncharacterized protein LOC111447045 OS=Cucurbita moschata OX=3662 GN=LOC111447045 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 5.2e-162
Identity = 304/387 (78.55%), Postives = 324/387 (83.72%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLA-DDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFGGF 60
           MA+SLDDGEFWLPPQFL+ DDDDQ+S   NKN  +N  GST FQSGR SFPLEFGTFGGF
Sbjct: 1   MAESLDDGEFWLPPQFLSDDDDDQESSATNKNNGRNSFGSTAFQSGRPSFPLEFGTFGGF 60

Query: 61  SDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCD 120
           SDFSS  ESLKGSSETESD ED VAGLTL MARST+DDG D+DN K RV AGSPQSTLCD
Sbjct: 61  SDFSSTAESLKGSSETESDLEDLVAGLTLHMARSTVDDGLDSDNAKERVLAGSPQSTLCD 120

Query: 121 MGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQV 180
           MGSGSGCSQGSSRGSPKGN KVPSPPATWDLLHAAAG+VARMRM E+HGVVH NRGA QV
Sbjct: 121 MGSGSGCSQGSSRGSPKGNCKVPSPPATWDLLHAAAGQVARMRMTENHGVVHQNRGASQV 180

Query: 181 SVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQN 240
           SVPV NSS+ TGFYQQLQAMQFQHLQQKE+MQRQN  V EQIN  AGYQQ QQIHQ+VQN
Sbjct: 181 SVPVKNSSSDTGFYQQLQAMQFQHLQQKEIMQRQNPTVAEQINYHAGYQQ-QQIHQIVQN 240

Query: 241 GMRGNRGLSSSSWLPPPQGSGMRALFLGPP-GGKRECAGTGVFLPRHTGAQSEPRKKPVK 300
           GMR  RGLSSS W  PPQG+G RALF+G   GGKRECAGTGVFLPRHT   S+PR+KP  
Sbjct: 241 GMRSGRGLSSSPWRQPPQGAGTRALFVGTSGGGKRECAGTGVFLPRHTATHSQPRRKP-- 300

Query: 301 ANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQINR 360
                      C TVLVPARVMQALNLNL+DICSQPHLQPVA  RFNS+NDVLLRLQ NR
Sbjct: 301 ----------DCPTVLVPARVMQALNLNLEDICSQPHLQPVAAARFNSDNDVLLRLQFNR 360

Query: 361 GAHHQKRNSRRQPPTDHEIKLPQEWTY 386
            A++QKRNSRRQ  T+HEIKLPQEWTY
Sbjct: 361 SANYQKRNSRRQAATEHEIKLPQEWTY 374

BLAST of Spg033529 vs. ExPASy TrEMBL
Match: A0A6J1F0R5 (uncharacterized protein LOC111441333 OS=Cucurbita moschata OX=3662 GN=LOC111441333 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.3e-157
Identity = 297/389 (76.35%), Postives = 322/389 (82.78%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADDDDQKS---CVGNKNTDQNRLGSTTFQSGRASFPLEFGTFG 60
           MADSLDDGEFWLPPQFLADDD+  S   C  NKN+  N L ST F   R SFP EFGTFG
Sbjct: 1   MADSLDDGEFWLPPQFLADDDNMPSPNTCATNKNSALNCLASTPFPPPRPSFPFEFGTFG 60

Query: 61  GFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTL 120
           GFSDFSSPGESLKGSSETESDEED +AG TLRMA+STIDDGFD+ N K  + +GSPQSTL
Sbjct: 61  GFSDFSSPGESLKGSSETESDEEDCLAGFTLRMAQSTIDDGFDSYNSKTSLLSGSPQSTL 120

Query: 121 CDMGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAP 180
           CDMGSGSGCSQ SSRGSPK N KVPSPPAT DLLHAAAGEVARMRMNES GV+  NRG  
Sbjct: 121 CDMGSGSGCSQVSSRGSPKSNCKVPSPPATCDLLHAAAGEVARMRMNESRGVISQNRGTS 180

Query: 181 QVSVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMV 240
           QVSVPV NSSTGTGFYQQLQAMQ  HL QKE+MQRQN+ VGEQ+NSPAGYQ+ QQ+H MV
Sbjct: 181 QVSVPVKNSSTGTGFYQQLQAMQVTHLNQKEIMQRQNLTVGEQMNSPAGYQR-QQVHVMV 240

Query: 241 QNGMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQS-EPRKKP 300
           QNG+RG RGLSSS+W+PPPQGSG RALFLG  G KRECAGTGVFLPRH+  QS E R+KP
Sbjct: 241 QNGVRGCRGLSSSAWIPPPQGSGTRALFLGTQGSKRECAGTGVFLPRHSSTQSDEQRRKP 300

Query: 301 VKANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQI 360
                       ACSTVLVPARVMQALNLNL+DICSQPH+QP+ GGR NSENDV+LRLQI
Sbjct: 301 ------------ACSTVLVPARVMQALNLNLEDICSQPHVQPMGGGRLNSENDVVLRLQI 360

Query: 361 NRGAHHQKRNSRRQPPTDHEIKLPQEWTY 386
           NRG +++KRN R Q PTDHEIKLPQEWTY
Sbjct: 361 NRGGNYEKRNGRGQLPTDHEIKLPQEWTY 376

BLAST of Spg033529 vs. ExPASy TrEMBL
Match: A0A6J1J2V3 (uncharacterized protein LOC111482180 OS=Cucurbita maxima OX=3661 GN=LOC111482180 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.9e-156
Identity = 293/388 (75.52%), Postives = 321/388 (82.73%), Query Frame = 0

Query: 1   MADSLDDGEFWLPPQFLADDDD---QKSCVGNKNTDQNRLGSTTFQSGRASFPLEFGTFG 60
           MADSLDDGEFWLPPQFLADDD+   Q +C  NKN+  N LGST F   R  FP EFGT G
Sbjct: 1   MADSLDDGEFWLPPQFLADDDNMPSQNTCATNKNSHLNCLGSTPFLPQRPFFPFEFGTSG 60

Query: 61  GFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTL 120
           GFSDFSSPGESLKGSSETESDEED +A +TLRMA+STIDDGFD+ N K  + +GSPQSTL
Sbjct: 61  GFSDFSSPGESLKGSSETESDEEDCLAEITLRMAQSTIDDGFDSYNSKASLLSGSPQSTL 120

Query: 121 CDMGSGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAP 180
           CDMGSGSGCSQ SSRGSPK N KVPSPPAT DLLHAAAGEVARM+MNES GV+  NRG  
Sbjct: 121 CDMGSGSGCSQVSSRGSPKSNCKVPSPPATCDLLHAAAGEVARMQMNESRGVISQNRGTS 180

Query: 181 QVSVPVNNSSTGTGFYQQLQAMQFQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMV 240
           QVSVPV NSSTGTGFYQQLQA+Q  HL QKE+MQRQN+ VGEQ+NSPA YQ+ QQ+H MV
Sbjct: 181 QVSVPVKNSSTGTGFYQQLQAVQVSHLNQKEIMQRQNLTVGEQMNSPAAYQR-QQVHVMV 240

Query: 241 QNGMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPV 300
           QNG+RG RGLSSS+W+PPPQGSG RALFLG  GGKRECAGTGVFLPRH+  QSE R+KP 
Sbjct: 241 QNGVRGCRGLSSSAWIPPPQGSGTRALFLGARGGKRECAGTGVFLPRHSSTQSEQRRKP- 300

Query: 301 KANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQPHLQPVAGGRFNSENDVLLRLQIN 360
                      ACSTVLVPARVMQALNLNL+DICSQPH+QP+ GGR NSE DVLLRLQIN
Sbjct: 301 -----------ACSTVLVPARVMQALNLNLEDICSQPHVQPMGGGRLNSEKDVLLRLQIN 360

Query: 361 RGAHHQKRNSRRQPPTDHEIKLPQEWTY 386
           RG +++KRN R Q PTDHEIKLPQEWTY
Sbjct: 361 RGGNYEKRNGRGQLPTDHEIKLPQEWTY 375

BLAST of Spg033529 vs. TAIR 10
Match: AT3G54000.1 (CONTAINS InterPro DOMAIN/s: Uncharacterised conserved protein UCP022260 (InterPro:IPR016802); Has 94 Blast hits to 94 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 94; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 124.8 bits (312), Expect = 1.5e-28
Identity = 138/413 (33.41%), Postives = 189/413 (45.76%), Query Frame = 0

Query: 5   LDDGEFWLPPQFLADDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLE----FGTFGGFS 64
           +DD EFWLP +FL DDD     V  +N   N +G        + FP E    FGTF    
Sbjct: 8   VDDAEFWLPTEFLTDDD---FLVEKEN---NSVGIDD-----SLFPYEPRHGFGTF---- 67

Query: 65  DFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCDM 124
                G ++K ++  E DEE ++AGLT +M  S++ D F         +         +M
Sbjct: 68  -----GSTVKPNTVKEDDEESFLAGLTRQMVMSSLKDDFSGGVCGNHAFPAGNDHKAWEM 127

Query: 125 G------SGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNR 184
                  +G+GC   + R +   NS+V S    WDL  AA     RM +N+     H+ R
Sbjct: 128 NRSPPCVAGTGCCCLNQRFNQNLNSRVSS----WDLYCAA----ERMSINDE--PYHSGR 187

Query: 185 G-----APQVSVPVNNSSTGTGF--------YQQLQAMQFQHLQQKELMQRQNMAV---- 244
           G     A   +   N+S+ GTG+        YQ+LQA+QFQ L+Q++LM+ +   V    
Sbjct: 188 GLLGSPAKLSATVKNHSNNGTGYYNNHQSLQYQKLQAIQFQQLKQQQLMKHRRQLVRQNR 247

Query: 245 GEQINSPAGYQQPQQIHQMVQNGMRGNRGLSSSSWLPP-PQGSGMRALFLGPPGGKRECA 304
           G ++N                N   G   LSSS+W    P+   MRA+F+G   GKR   
Sbjct: 248 GVRVNG---------------NKNVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGST 307

Query: 305 GTGVFLPR---HTGAQSEPRKKPVKANHEFLSQSTACSTVLVPARVMQALNLNLDDICSQ 364
           GTGVFLPR   HT +++E R+KP              STVLVPAR+ Q LNLNL      
Sbjct: 308 GTGVFLPRSVNHT-SRTETREKP------------TISTVLVPARLAQVLNLNLG----- 352

Query: 365 PHLQPVAGGRFNSENDVLLRLQINRGA-HHQKRNSRRQPPTDHEIKLPQEWTY 386
              +PV      + NDV  R + N G    Q     R   +  E +LP EW Y
Sbjct: 368 ---EPVRSTA--TLNDVSWRQRSNNGGFSSQMVGGVRAEQSVQEPRLPSEWAY 352

BLAST of Spg033529 vs. TAIR 10
Match: AT3G54000.2 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 102.4 bits (254), Expect = 8.1e-22
Identity = 109/325 (33.54%), Postives = 154/325 (47.38%), Query Frame = 0

Query: 5   LDDGEFWLPPQFLADDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLE----FGTFGGFS 64
           +DD EFWLP +FL DDD     V  +N   N +G        + FP E    FGTF    
Sbjct: 8   VDDAEFWLPTEFLTDDD---FLVEKEN---NSVGIDD-----SLFPYEPRHGFGTF---- 67

Query: 65  DFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCDM 124
                G ++K ++  E DEE ++AGLT +M  S++ D F         +         +M
Sbjct: 68  -----GSTVKPNTVKEDDEESFLAGLTRQMVMSSLKDDFSGGVCGNHAFPAGNDHKAWEM 127

Query: 125 G------SGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNR 184
                  +G+GC   + R +   NS+V S    WDL  AA     RM +N+     H+ R
Sbjct: 128 NRSPPCVAGTGCCCLNQRFNQNLNSRVSS----WDLYCAA----ERMSINDE--PYHSGR 187

Query: 185 G-----APQVSVPVNNSSTGTGF--------YQQLQAMQFQHLQQKELMQRQNMAV---- 244
           G     A   +   N+S+ GTG+        YQ+LQA+QFQ L+Q++LM+ +   V    
Sbjct: 188 GLLGSPAKLSATVKNHSNNGTGYYNNHQSLQYQKLQAIQFQQLKQQQLMKHRRQLVRQNR 247

Query: 245 GEQINSPAGYQQPQQIHQMVQNGMRGNRGLSSSSWLPP-PQGSGMRALFLGPPGGKRECA 299
           G ++N                N   G   LSSS+W    P+   MRA+F+G   GKR   
Sbjct: 248 GVRVNG---------------NKNVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGST 286

BLAST of Spg033529 vs. TAIR 10
Match: AT3G54000.3 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 102.4 bits (254), Expect = 8.1e-22
Identity = 109/325 (33.54%), Postives = 154/325 (47.38%), Query Frame = 0

Query: 5   LDDGEFWLPPQFLADDDDQKSCVGNKNTDQNRLGSTTFQSGRASFPLE----FGTFGGFS 64
           +DD EFWLP +FL DDD     V  +N   N +G        + FP E    FGTF    
Sbjct: 8   VDDAEFWLPTEFLTDDD---FLVEKEN---NSVGIDD-----SLFPYEPRHGFGTF---- 67

Query: 65  DFSSPGESLKGSSETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCDM 124
                G ++K ++  E DEE ++AGLT +M  S++ D F         +         +M
Sbjct: 68  -----GSTVKPNTVKEDDEESFLAGLTRQMVMSSLKDDFSGGVCGNHAFPAGNDHKAWEM 127

Query: 125 G------SGSGCSQGSSRGSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNR 184
                  +G+GC   + R +   NS+V S    WDL  AA     RM +N+     H+ R
Sbjct: 128 NRSPPCVAGTGCCCLNQRFNQNLNSRVSS----WDLYCAA----ERMSINDE--PYHSGR 187

Query: 185 G-----APQVSVPVNNSSTGTGF--------YQQLQAMQFQHLQQKELMQRQNMAV---- 244
           G     A   +   N+S+ GTG+        YQ+LQA+QFQ L+Q++LM+ +   V    
Sbjct: 188 GLLGSPAKLSATVKNHSNNGTGYYNNHQSLQYQKLQAIQFQQLKQQQLMKHRRQLVRQNR 247

Query: 245 GEQINSPAGYQQPQQIHQMVQNGMRGNRGLSSSSWLPP-PQGSGMRALFLGPPGGKRECA 299
           G ++N                N   G   LSSS+W    P+   MRA+F+G   GKR   
Sbjct: 248 GVRVNG---------------NKNVGPVDLSSSAWSNQFPRRDVMRAVFIGDHTGKRGST 286

BLAST of Spg033529 vs. TAIR 10
Match: AT2G39870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55690.1); Has 73 Blast hits to 71 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 2; Plants - 69; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 82.0 bits (201), Expect = 1.1e-15
Identity = 83/263 (31.56%), Postives = 120/263 (45.63%), Query Frame = 0

Query: 49  FPLEFGTFGGFSDFSSPGESLKGSSETESDEEDYVAGLTLRMARST--IDDGFDTDNPKG 108
           FP EF +      F+SPG+S +   E+  DEED++AGLT R+A ST  +         K 
Sbjct: 35  FPYEFDSPSFSPGFTSPGDSTETEDESSDDEEDFLAGLTRRLAPSTQRLPSPLFKSEEKR 94

Query: 109 RVYAGSPQSTLCDMGSGSGCSQGSSRGSPKGNSKVPSPPA---------TWDLLHAAAGE 168
           +V A SPQSTL    SG G    S   SP     +PSPPA          WD++ AAAGE
Sbjct: 95  QVAATSPQSTL----SGLGSFSNSGSRSP----ILPSPPAPTSSFRRDNAWDVISAAAGE 154

Query: 169 VARMRMNESHGVVHNNRGAPQVSVPVNNSSTGTGF-YQQLQAMQFQHLQQKELMQRQNMA 228
           VAR+++  S+   H     P+  +   N++      +Q+L    +    Q      +N  
Sbjct: 155 VARLKLG-SYEPHHLPLQTPESLLRRQNAAIHAELQHQRLIEQMWLCSAQSRFKLSENRI 214

Query: 229 VGEQINSPAGYQQPQQIHQMVQNGMRGNRGLSSSSWLPPPQGSGMRALFLGPPGGKRECA 288
               +N    ++ P+ + +            ++ +WLPP Q +            KR  A
Sbjct: 215 PRRVVNEEGLFENPRYVRR------------NNPTWLPPQQAAAPL---------KRPSA 267

Query: 289 GTGVFLPRH--TGAQSEPRKKPV 298
           GTGVFLPR   + A S+  K PV
Sbjct: 275 GTGVFLPRRYPSAAPSDSLKTPV 267

BLAST of Spg033529 vs. TAIR 10
Match: AT5G59050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G54000.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 62.4 bits (150), Expect = 9.2e-10
Identity = 83/329 (25.23%), Postives = 136/329 (41.34%), Query Frame = 0

Query: 73  SETESDEEDYVAGLTLRMARSTIDDGFDTDNPKGRVYAGSPQSTLCDMGSGSGCSQGSSR 132
           ++ E +E++Y+  LT +M    + D        G   +GSPQSTL            S  
Sbjct: 46  AKNEDEEDEYITELTRQMTNYMLQDDEKHQKSCGS-GSGSPQSTLWS-------PFASGL 105

Query: 133 GSPKGNSKVPSPPATWDLLHAAAGEVARMRMNESHGVVHNNRGAPQVSVPVNNSSTGTGF 192
            SP G S+ PSPP T      A   V +         +        V++P  +       
Sbjct: 106 SSPIGPSREPSPPLT-----PATVPVEK---------IMTKIDTKPVTIPFQSKQALID- 165

Query: 193 YQQLQAMQ--FQHLQQKELMQRQNMAVGEQINSPAGYQQPQQIHQMVQNGMRGNRGLSSS 252
             Q++++Q  FQ +++++  +RQ        N+     + +  H + QN           
Sbjct: 166 -DQIRSIQANFQKIKKEKEKERQR-------NADVLGHKARNYHHLHQN----------- 225

Query: 253 SWLPPPQGSGMRALFLGPPGGKRECAGTGVFLPRHTGAQSEPRKKPVKANHEFLSQSTAC 312
                   SG++A+F+   G +    GTGVFLPR  G   E RKK            + C
Sbjct: 226 ----QRPRSGVKAVFVDGSGSRTGSGGTGVFLPRGHGTVVESRKK------------SGC 285

Query: 313 STVLVPARVMQALNLNLDDI----CSQPHLQPVAGGRFNSENDVLLR-------LQINRG 372
           STV++PARV++AL ++ D +         + P       S N+  ++        ++  G
Sbjct: 286 STVIIPARVVEALKVHFDKLGVPSTFSSDIPPFHDALLVSMNNKKIKSNKNTSLSRVQSG 312

Query: 373 AHHQ---KRNSRRQPPTDHEIKLPQEWTY 386
           + ++      S ++PP D    LPQEWTY
Sbjct: 346 SPYEMEMSAESHQEPPAD----LPQEWTY 312