Spg033351 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033351
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Steroid 5-alpha-reductase DET2
Location	scaffold5: 1471054 .. 1473947 (+)
RNA-Seq Expression	Spg033351
Synteny	Spg033351

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAATATCTGCTATAATGTAAATCAAGTTGAAGTTCAAGCATGTGCTGTTCTCTGCAGTGGTTTTCTGAGTTCAGCTAATCGATCTTTGCAGTTTTGTTCTTTGAAATTGAGGATCACTAGTTTGTGGTTTTTAAGAAAATGCACTATTAGCAAGTTTATTGATGGAGGATAGATCAACCAACTCTTTTACAAGCCATAAATGATAGGAGAGAACCTAATCTATTCTTACATATCACTTGAAGACTATGTCCTAGAATATATTCTCCTAGCCTACTTGATATGGGGCCGTCTTCTTCGAAGTTTTGGGAGCATCAGTTCATTTATTCTACACCATTTTCTATTGATAACAGATTAGTTTGTAGCCTATGTCCAATTTTCTAGCTTGGAAATATTAACATCAAGTTGAGAACTATAGTCATTTAATTCGAAGAAACTTTAGTTACTCCAGCAGTCTCTTTGTTTCAAAACAAATCTTGAGTAATTCCACTCTCAGGCATTGTTCAAATGGTAGAAGATTATCCACCCGCAGAATTGTTCTGCTCTCTGCTTGGTATTATCAAATTATGAATAAACTGATATGTTCGTCATTTTCATCTGACAGAAGCTCATCTGATAAGAATTACCACAATTGTTTTTGCATTCCTGTTGGAGAATCAGATCACCCCAGAATCTCTTTCCTCCACATGTACTGCTTCCTTCTTCATACAGGATCGTTCCATCCACAGTTGGCCGTTCAAGTCACTTCCAGCAGTTCTGCTTTGTGTTGAATGCATATTTTGCTGCTTTGGATGGTTTCAAGCCATTTTTCTTTCCCCCAAGCTTAAAATTCTCTGAAGACCATTTAGCATCCTCAGGTTCGGCTGCTTAACTTGGAAAATATAACCAGCAGAACTCATTCAAAGGCTCATTGATGCTCAGTTATGCAATCTACTTACAAATCTGATTCTCAAACTTGGCTTACAAACTACAGTGTAGTGCATCTAATGGTATTCTGTAAACTGATATTTATGTTTATTTTGTACAATGAACTGCATCAACAAAGTTATACTTCTTTCTTTTGTACGCCTCATCATTGAAATCTGACTATCTGAGTTAATTCTCCTTCGCCATATCCATCCCCATCCAATCCATGTTAAGTTGATTCATTCTGCAGCAGCTTAAATAGTTAGAGAAATTGTGACTTTAGAGTTTAGATATGAAAAGAAAGCATGAAATAGTTGGGAATGGGGAAAGGAGGAAGAAGATTGATGAAAACAAGTCAGTTCTGGTTCACAACCAGAAATCTTATCTGATTCTCTTGACACTTTATAGATTATCTCAATGGCAAGCGATTTAGAAACTCAAACAGGAGCACAAGGAAATACAGTGAGCAAGGGGAATTGAAAAGGGGGAAAGGAAAAAGTAGGAGATAAGGAAACATCAGCATGTGGGCATTTGATAGAAACAAAAGTTAGATGTTAAGAGGCCTGTATGCATTTATGGAGCCATATGAATATGTTTTCCTTTTGATGGTTCTTGAAGAATGATAGCAAAAGTTCTGTTACTTCCATGGGTGGAAGTTAGATTTCCCAGATGTGTACTGAGATCTAAGGTCACTGCCATTATTGAGGCGTGACAGGCAGGAGATGGACTTGCACACAAAGTCCAACCACAATTGTTTTTCTTATTAACTTTTTCAGTTTGGATCCTTTGCCTGATGGCAACTACGATATTAAATGCAGCCTAAGCAGGTGCAGGTCCACGTCTTAAGAAAAGTAATATGATTCAAAAAGTCCCACTTAACTTTTCAATTAGACCATAAATTTGCTAAAATTGTTTTGGTCCTTGAGTTTTGAGAAATAGAAACATTTGGTCTCTGAATGTTCAAAATAGATCTTTTTAGTCCAAGGTCCATTTTGAAAACTCATTTCAAAATGGACTTTTTAAACTTATTTCTTGAAAACTCAGGAACAAGCACTCAATTTTTTTTCCCTAAAATTATTCATTGGCAGTAGATATTGAGTAATCAGTATTGATTTTGTGCCAGATTTCCTCACTTCCAAGTTCCAACTCAATTTGAAACGAACCCACAACCAGAATCCTCGAATCCCATCACTTATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTTCTCGCCCTGTACTTCATCGGACCACCGACCGCCGTCGCCCTGAGGTTCCTCCAGGCGCCGTACGGGAAGCACAGCCGGCCTGGTTGGGGCCCGACCATGTCTGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACCCTATGGCTTACCCTCCTCCTCTTCCCCTCCGGCGATCACTCCTCCGATTCCAAGCCTCTCCTTCTCATCTCCCCCTTCCTTCTCCACTACCTCCACCGCACCTGCCTCTACCCGATTCGCCTTCTCCGGAGCGGACCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGCTGTGGCGCTCATGGCATTCGGGTTTAATCTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGAGATGGGTGGTTCTGGTGGCGGTTTTTGGGTGGAGGAGTGGTTTTTCTGTCGGGAATGGCGGTGAATCTCTGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGGCCGCCGCAGCCGCCGGCGGTGAGTATAAGGTGCCGAGAGGAGGGTGGTTTGAATTGGTAAGTTGCCCTAATTACATGGGGGAGATTGTGGAATGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCCGGGCTGGGGTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAACAAGTTTGGGGAGGATTATCCAAGAAATAGGAAGGCCGTTATTCCCTTCTTGTATTGA

mRNA sequence

ATGGATAATATCTGCTATAATGTAAATCAAGTTGAAGTTCAAGCATGTGCTGTTCTCTGCAGTGGTTTTCTGAGTTCAGCTAATCGATCTTTGCAGATCGTTCCATCCACAGTTGGCCGTTCAAGTCACTTCCAGCAGTTCTGCTTTGTGTTGAATGCATATTTTGCTGCTTTGGATGGTTTCAAGCCATTTTTCTTTCCCCCAAGCTTAAAATTCTCTGAAGACCATTTAGCATCCTCAGATTTCCTCACTTCCAAGTTCCAACTCAATTTGAAACGAACCCACAACCAGAATCCTCGAATCCCATCACTTATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTTCTCGCCCTGTACTTCATCGGACCACCGACCGCCGTCGCCCTGAGGTTCCTCCAGGCGCCGTACGGGAAGCACAGCCGGCCTGGTTGGGGCCCGACCATGTCTGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACCCTATGGCTTACCCTCCTCCTCTTCCCCTCCGGCGATCACTCCTCCGATTCCAAGCCTCTCCTTCTCATCTCCCCCTTCCTTCTCCACTACCTCCACCGCACCTGCCTCTACCCGATTCGCCTTCTCCGGAGCGGACCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGCTGTGGCGCTCATGGCATTCGGGTTTAATCTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGAGATGGGTGGTTCTGGTGGCGGTTTTTGGGTGGAGGAGTGGTTTTTCTGTCGGGAATGGCGGTGAATCTCTGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGGCCGCCGCAGCCGCCGGCGGTGAGTATAAGGTGCCGAGAGGAGGGTGGTTTGAATTGGTAAGTTGCCCTAATTACATGGGGGAGATTGTGGAATGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCCGGGCTGGGGTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAACAAGTTTGGGGAGGATTATCCAAGAAATAGGAAGGCCGTTATTCCCTTCTTGTATTGA

Coding sequence (CDS)

ATGGATAATATCTGCTATAATGTAAATCAAGTTGAAGTTCAAGCATGTGCTGTTCTCTGCAGTGGTTTTCTGAGTTCAGCTAATCGATCTTTGCAGATCGTTCCATCCACAGTTGGCCGTTCAAGTCACTTCCAGCAGTTCTGCTTTGTGTTGAATGCATATTTTGCTGCTTTGGATGGTTTCAAGCCATTTTTCTTTCCCCCAAGCTTAAAATTCTCTGAAGACCATTTAGCATCCTCAGATTTCCTCACTTCCAAGTTCCAACTCAATTTGAAACGAACCCACAACCAGAATCCTCGAATCCCATCACTTATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTTCTCGCCCTGTACTTCATCGGACCACCGACCGCCGTCGCCCTGAGGTTCCTCCAGGCGCCGTACGGGAAGCACAGCCGGCCTGGTTGGGGCCCGACCATGTCTGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACCCTATGGCTTACCCTCCTCCTCTTCCCCTCCGGCGATCACTCCTCCGATTCCAAGCCTCTCCTTCTCATCTCCCCCTTCCTTCTCCACTACCTCCACCGCACCTGCCTCTACCCGATTCGCCTTCTCCGGAGCGGACCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGCTGTGGCGCTCATGGCATTCGGGTTTAATCTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGAGATGGGTGGTTCTGGTGGCGGTTTTTGGGTGGAGGAGTGGTTTTTCTGTCGGGAATGGCGGTGAATCTCTGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGGCCGCCGCAGCCGCCGGCGGTGAGTATAAGGTGCCGAGAGGAGGGTGGTTTGAATTGGTAAGTTGCCCTAATTACATGGGGGAGATTGTGGAATGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCCGGGCTGGGGTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAACAAGTTTGGGGAGGATTATCCAAGAAATAGGAAGGCCGTTATTCCCTTCTTGTATTGA

Protein sequence

MDNICYNVNQVEVQACAVLCSGFLSSANRSLQIVPSTVGRSSHFQQFCFVLNAYFAALDGFKPFFFPPSLKFSEDHLASSDFLTSKFQLNLKRTHNQNPRIPSLMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY

Homology

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BLAST of Spg033351 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 496.9 bits (1278), Expect = 1.5e-136
Identity = 235/283 (83.04%), Postives = 252/283 (89.05%), Query Frame = 0


Query: 85  SKFQLNLKRTHNQNPRIPSLMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPG 144
           S+   NL +  + NP  P++MG DQALF NSLLALY IGPPT V+LRFLQAPYGKH RPG
Sbjct: 15  SQLDSNL-QPESSNPS-PTVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG 74

Query: 145 WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLL 204
           WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSK LLLISPFLLHYL+RTC YPIRLL
Sbjct: 75  WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLL 134

Query: 205 RSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLS 264
            SG RRN  AGFPV VA+MAFGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ 
Sbjct: 135 WSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVM 194

Query: 265 GMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWA 324
           GMAVN+WSDGVLVGLKR    +GG YKVP+GGWFELVSCPNY+GEI+EWLGW VMCGSWA
Sbjct: 195 GMAVNIWSDGVLVGLKR----SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWA 254

Query: 325 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           GLGFFLYTCANLVPRARANHTWYLNKFGEDYP NRKAVIPFLY
Sbjct: 255 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

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BLAST of Spg033351 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 496.5 bits (1277), Expect = 2.0e-136
Identity = 232/263 (88.21%), Postives = 243/263 (92.40%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           MGSDQALFHNSLL LY IGP   VALRFLQAPYGKH++PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFPSGDHSS+SK LLLISPFLLHY +RTCLYPIRLLRSG RRNTAAGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           FGFNLLNSYLQTRSVSHY++Y+GDGWFWWRF GGGVVFL GM+VN+WSDGVLVGLKR   
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKR--- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
             GG YKVP G WFELVSCPNY+GEIVEWLGWAVMCGSWAGL FFLYTCANLVPRARANH
Sbjct: 181 -GGGGYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYPRNRKAVIPFLY
Sbjct: 241 TWYLNKFGEDYPRNRKAVIPFLY 259

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BLAST of Spg033351 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 493.0 bits (1268), Expect = 2.2e-135
Identity = 235/283 (83.04%), Postives = 249/283 (87.99%), Query Frame = 0


Query: 85  SKFQLNLKRTHNQNPRIPSLMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPG 144
           S+   NL +  + NP  P++MG DQALFHNSLLALY IGPPT V+LRFLQAPYGKH RPG
Sbjct: 15  SQLDSNL-QPESSNPS-PTMMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG 74

Query: 145 WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLL 204
           WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SK LLLISPFLLHYL+RTC YPIRLL
Sbjct: 75  WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLL 134

Query: 205 RSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLS 264
            SG RRN   GFPV VA+MAFGFNLLNSY+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ 
Sbjct: 135 WSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVM 194

Query: 265 GMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWA 324
           GMAVN+WSDGVLVGLKR     GG YKVP GGWFELVSCPNY+GEI+EWLGWAVMCGSWA
Sbjct: 195 GMAVNIWSDGVLVGLKR----RGGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWA 254

Query: 325 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           GLGFFLYTCANLVPRARANHTWYLNKFGE YP NRKAVIPFLY
Sbjct: 255 GLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Spg033351 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 492.7 bits (1267), Expect = 2.8e-135
Identity = 229/263 (87.07%), Postives = 241/263 (91.63%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           MG DQALF NSLLALY IGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFPSGDHSSDSK LLLISPFLLHYL+RTC YPIRLL SG RRN  AGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ GMAVN+WSDGVLVGLKR   
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR--- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
            +GG YKVP+GGWFELVSCPNY+GEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANH
Sbjct: 181 -SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYP NRKAVIPFLY
Sbjct: 241 TWYLNKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Spg033351 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 487.3 bits (1253), Expect = 1.2e-133
Identity = 230/263 (87.45%), Postives = 240/263 (91.25%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           M SDQ LFHNSLLALY IGPPTAV+LRFLQAPYGKH+RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFPSGD+SS+ K LLLIS FLLHYL+RTCLYPIRLLRSG RRN+A GFPV VALMA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           FGFNLLNSYLQTRSVSHYVDYD D WFWWRF GG VVF+ GMAVN+WSDGVLV LK    
Sbjct: 121 FGFNLLNSYLQTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLK---- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
            AGG YKVP GGWFELVSCPNY+GEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH
Sbjct: 181 MAGGGYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYPR+RKAVIPFLY
Sbjct: 241 TWYLNKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Spg033351 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 5.8e-99
Identity = 175/264 (66.29%), Postives = 199/264 (75.38%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           M SDQ LFH  LL LY I  PT ++L FLQAPYGKH+RPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LT  LFPSG H  + K  LLISPFL HY +RT LYP+RL R+        GFPV+VA MA
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRLARN---TTQTRGFPVSVAFMA 120

Query: 225 FGFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAA 284
           FGFNLLN YLQ R VSHY  DY+ +  FWWRFL G ++F+ GM VN+ +D VLVGLK+  
Sbjct: 121 FGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKK-- 180

Query: 285 AAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARAN 344
               G YK+PRGG FELVSCPNY GEI+EW GWAVM  SW G GFFLYTCANL+PRARA 
Sbjct: 181 -QGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARAT 240

Query: 345 HTWYLNKFGEDYPRNRKAVIPFLY 368
             WYL KF +DYP++RKAVIPF+Y
Sbjct: 241 RLWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of Spg033351 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 1.9e-97
Identity = 167/263 (63.50%), Postives = 200/263 (76.05%), Query Frame = 0


Query: 107 SDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLWLT 166
           +D+  F   LL L F GPPTAV L+FLQAPYGKH+R GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 167 LLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSG-PRRNTAAGFPVAVALMAF 226
           LLLFP G H+ + K LLL SP+L+HY HRT +YP+RL RS  P      GFP+ +A +AF
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN--GFPITIAALAF 124

Query: 227 GFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 286
            FNLLN Y+Q R VSHY  DY+   WFWWRF+ G VVF++GM +N+ SD  LV LK+   
Sbjct: 125 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 184

Query: 287 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 346
              G Y +PRGGWFELVSCPNY GE +EWLGWAVM  SWAG+GFFLYTC+NL PRARA+H
Sbjct: 185 ---GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASH 244

Query: 347 TWYLNKFGEDYPRNRKAVIPFLY 368
            WY+ KF E+YP+ RKAVIPF+Y
Sbjct: 245 KWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Spg033351 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.1e-81
Identity = 149/265 (56.23%), Postives = 192/265 (72.45%), Query Frame = 0


Query: 104 LMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKH-SRPGWGPTMSAPLAWFLMESPT 163
           +  SD+ LF+  +  +  +  PT +  +F  +PYGKH +    G T+S P+AW  MESPT
Sbjct: 1   MFSSDENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPT 60

Query: 164 LWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVAL 223
           LWLT+++F  G + ++    LLISP+L HY +RT +YP+R LRS   RNT   FP+ +A+
Sbjct: 61  LWLTIIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLR-LRS---RNTKNNFPLNIAV 120

Query: 224 MAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRA 283
            AF FNLLN+Y+Q+R VSHY +Y  D WFW RF  G V+F SGM +N+W+DGVL+GLK  
Sbjct: 121 TAFIFNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLK-- 180

Query: 284 AAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARA 343
             + GG YK+PRGG F+ VS PNY+GEI+EWLGWA+M  SWAGL FF+YTCANLVPRA +
Sbjct: 181 --SQGGGYKIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVS 240

Query: 344 NHTWYLNKFGEDYPRNRKAVIPFLY 368
           NH WYL KFGEDYP+NRKAV PFLY
Sbjct: 241 NHKWYLQKFGEDYPKNRKAVFPFLY 257

[Close]

BLAST of Spg033351 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 2.6e-35
Identity = 94/241 (39.00%), Postives = 136/241 (56.43%), Query Frame = 0


Query: 128 VALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKPLLLI 187
           V L+ + + YG++S    G  + A  AWFL E P++ W L   + P+     +    +L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 188 SPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDY 247
           + FL+HY+ RT ++P+ L+R G          +   ++AF F  LN YLQ+R +S +  Y
Sbjct: 85  AMFLIHYVQRTLVFPV-LIRGGKPT------LLFTFVLAFLFCTLNGYLQSRYLSQFAVY 144

Query: 248 DGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPN 307
             D      FL G  ++L GM +N+ SD +L  L++        YK+PRGG FE VS  N
Sbjct: 145 AEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETG---YKIPRGGLFEYVSSAN 204

Query: 308 YMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPF 367
           Y GE+VEW G+A+   S  G+ F L+T   L  RAR +H WYL KF EDYP+ RK +IPF
Sbjct: 205 YFGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYPKTRKILIPF 254

[Close]

BLAST of Spg033351 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.5e-35
Identity = 93/238 (39.08%), Postives = 131/238 (55.04%), Query Frame = 0


Query: 137 YGKHSRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLL-------LISPFL 196
           YG+H+ P +   + A  AW + E P+L L     P   ++S+S P L       L++ FL
Sbjct: 42  YGRHASPSFRVRVPARAAWVVQELPSLAL-----PLYQYASESAPRLRSAPNCILLAMFL 101

Query: 197 LHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDG 256
           +HY HR  +YP  L+R G         P+    MA  F   N YLQ+R +SH+  Y  D 
Sbjct: 102 VHYGHRCLIYPF-LMRGG------KPMPLLACTMAIMFCTFNGYLQSRYLSHWAVYADDW 161

Query: 257 WFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGE 316
               RFL G  ++L+GM +N+ SD +L  L++        YK+PRGG FE V+  NY GE
Sbjct: 162 VTDPRFLIGFGLWLAGMLINIHSDHILRNLRKPGDTG---YKIPRGGLFEYVTAANYFGE 221

Query: 317 IVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           I+EW G+A+   S  G  F  +T   L  RA+ +H WYL KF E+YP+ RK +IPFL+
Sbjct: 222 IMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKF-EEYPKFRKILIPFLF 263

[Close]

BLAST of Spg033351 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 7.3e-137
Identity = 235/283 (83.04%), Postives = 252/283 (89.05%), Query Frame = 0


Query: 85  SKFQLNLKRTHNQNPRIPSLMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPG 144
           S+   NL +  + NP  P++MG DQALF NSLLALY IGPPT V+LRFLQAPYGKH RPG
Sbjct: 15  SQLDSNL-QPESSNPS-PTVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG 74

Query: 145 WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLL 204
           WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSK LLLISPFLLHYL+RTC YPIRLL
Sbjct: 75  WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLL 134

Query: 205 RSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLS 264
            SG RRN  AGFPV VA+MAFGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ 
Sbjct: 135 WSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVM 194

Query: 265 GMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWA 324
           GMAVN+WSDGVLVGLKR    +GG YKVP+GGWFELVSCPNY+GEI+EWLGW VMCGSWA
Sbjct: 195 GMAVNIWSDGVLVGLKR----SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWA 254

Query: 325 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           GLGFFLYTCANLVPRARANHTWYLNKFGEDYP NRKAVIPFLY
Sbjct: 255 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of Spg033351 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 1.0e-135
Identity = 235/283 (83.04%), Postives = 249/283 (87.99%), Query Frame = 0


Query: 85  SKFQLNLKRTHNQNPRIPSLMGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPG 144
           S+   NL +  + NP  P++MG DQALFHNSLLALY IGPPT V+LRFLQAPYGKH RPG
Sbjct: 15  SQLDSNL-QPESSNPS-PTMMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG 74

Query: 145 WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLL 204
           WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SK LLLISPFLLHYL+RTC YPIRLL
Sbjct: 75  WGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLL 134

Query: 205 RSGPRRNTAAGFPVAVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLS 264
            SG RRN   GFPV VA+MAFGFNLLNSY+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ 
Sbjct: 135 WSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVM 194

Query: 265 GMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWA 324
           GMAVN+WSDGVLVGLKR     GG YKVP GGWFELVSCPNY+GEI+EWLGWAVMCGSWA
Sbjct: 195 GMAVNIWSDGVLVGLKR----RGGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWA 254

Query: 325 GLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           GLGFFLYTCANLVPRARANHTWYLNKFGE YP NRKAVIPFLY
Sbjct: 255 GLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Spg033351 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.4e-135
Identity = 229/263 (87.07%), Postives = 241/263 (91.63%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           MG DQALF NSLLALY IGPPT V+LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFPSGDHSSDSK LLLISPFLLHYL+RTC YPIRLL SG RRN  AGFPV VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRF GGGVVF+ GMAVN+WSDGVLVGLKR   
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKR--- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
            +GG YKVP+GGWFELVSCPNY+GEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANH
Sbjct: 181 -SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYP NRKAVIPFLY
Sbjct: 241 TWYLNKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Spg033351 vs. ExPASy TrEMBL
Match: A0A6J1CX04 (Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.9e-133
Identity = 227/263 (86.31%), Postives = 240/263 (91.25%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           MGSDQALF NSLLALY IGPPTAVALRFLQAPYGKH+RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFP GDHSS+ KP+LLISPFLLHYLHRTCLYPIRLLR   +RN+AAGFPVAVALMA
Sbjct: 61  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLLR---QRNSAAGFPVAVALMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           FGFN+LNSYLQTRSVSHY  Y+GDGWFWWRF  G VVF +GM VN+WSDGVLVGLKR   
Sbjct: 121 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKR--- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
            AGG YK+P GGWFELVSCPNY+GE+VEWLGWAVMC SWAGLGFFLYTCANLVPRARANH
Sbjct: 181 -AGGGYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYP  RKA+IPFLY
Sbjct: 241 TWYLNKFGEDYPTTRKAIIPFLY 256

[Close]

BLAST of Spg033351 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.9e-132
Identity = 227/263 (86.31%), Postives = 239/263 (90.87%), Query Frame = 0


Query: 105 MGSDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLW 164
           M SDQ LFHNSLLALY IGPPTA++LRFLQAPYGKH+RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 165 LTLLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSGPRRNTAAGFPVAVALMA 224
           LTLLLFPSGD+SS+ K LLLIS FLLHYL+RTCLYPIRLLRSG RRN+A GFPV VALMA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 225 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 284
           F FNLLNSYLQTRSVSHY+DYD D WFWWRF GG VVF+ GMAVN+WSDGVLV LK    
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLK---- 180

Query: 285 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 344
            AGG YKVP GGWFELVSCPNY+GEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH
Sbjct: 181 MAGGGYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 240

Query: 345 TWYLNKFGEDYPRNRKAVIPFLY 368
           TWYLNKFGEDYPR+RKAVIPFLY
Sbjct: 241 TWYLNKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Spg033351 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-98
Identity = 167/263 (63.50%), Postives = 200/263 (76.05%), Query Frame = 0


Query: 107 SDQALFHNSLLALYFIGPPTAVALRFLQAPYGKHSRPGWGPTMSAPLAWFLMESPTLWLT 166
           +D+  F   LL L F GPPTAV L+FLQAPYGKH+R GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 167 LLLFPSGDHSSDSKPLLLISPFLLHYLHRTCLYPIRLLRSG-PRRNTAAGFPVAVALMAF 226
           LLLFP G H+ + K LLL SP+L+HY HRT +YP+RL RS  P      GFP+ +A +AF
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN--GFPITIAALAF 124

Query: 227 GFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFLGGGVVFLSGMAVNLWSDGVLVGLKRAAA 286
            FNLLN Y+Q R VSHY  DY+   WFWWRF+ G VVF++GM +N+ SD  LV LK+   
Sbjct: 125 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 184

Query: 287 AAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANH 346
              G Y +PRGGWFELVSCPNY GE +EWLGWAVM  SWAG+GFFLYTC+NL PRARA+H
Sbjct: 185 ---GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASH 244

Query: 347 TWYLNKFGEDYPRNRKAVIPFLY 368
            WY+ KF E+YP+ RKAVIPF+Y
Sbjct: 245 KWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Spg033351 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-14
Identity = 39/112 (34.82%), Postives = 70/112 (62.50%), Query Frame = 0


Query: 256 LGGGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLG 315
           L G V+F+ G+  NL+   +L  L++       EYK+P+GG F+++ CP+Y+ EI+ +  
Sbjct: 160 LAGVVMFVVGIVGNLYHHVLLAKLRKEDGKK--EYKIPKGGLFDIIICPHYLFEILVFWS 219

Query: 316 WAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           + ++  +     F + T   L+ R+ A  TWYL+KF +D+P++ KA+IPF++
Sbjct: 220 FFLISQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFPKHIKALIPFVF 268

[Close]

BLAST of Spg033351 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 77.0 bits (188), Expect = 3.5e-14
Identity = 45/115 (39.13%), Postives = 60/115 (52.17%), Query Frame = 0


Query: 258 GGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWA 317
           GG +FL G         +L  L+   + A  EY +P G WFE+VSCP+++ EIV +LG  
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQA-KEYIIPYGDWFEMVSCPHFLAEIVLYLGLL 270

Query: 318 VMCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           +  G      W   GF     ANL   A   H WYL KF E+YP +R A+ P +Y
Sbjct: 271 ISSGGTDISIWLLFGF---VAANLTYAAGETHRWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of Spg033351 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 258 GGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWA 317
           GG +FL G         +L  L+   + A  EY +P G WF +VS P+++ EIV + G  
Sbjct: 234 GGAIFLWGWIHQRRCHAILGSLRENPSQA-KEYIIPYGDWFGMVSSPHFLAEIVLYAGLL 293

Query: 318 VMCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           +  G      W   GF     ANL   A   H WYL KF E+YP NR A+ P++Y
Sbjct: 294 IASGGTDITIWLLFGF---VAANLTYAAGETHRWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of Spg033351 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 1.1e-12
Identity = 43/115 (37.39%), Postives = 58/115 (50.43%), Query Frame = 0


Query: 258 GGVVFLSGMAVNLWSDGVLVGLKRAAAAAGGEYKVPRGGWFELVSCPNYMGEIVEWLGWA 317
           GG +FL G         +L  L+   + A  EY +P G WF +VS P+++ EIV + G  
Sbjct: 233 GGAIFLWGWIHQRRCHAILGSLRENPSQA-KEYIIPYGDWFGMVSSPHFLAEIVLYAGLL 292

Query: 318 VMCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPRNRKAVIPFLY 368
           +  G      W   GF     ANL   A   H WYL KF E+YP NR A+ P++Y
Sbjct: 293 IASGGTDITIWLLFGF---VAANLTYAAGETHRWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of Spg033351 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442471.1	1.5e-136	83.04	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
XP_038905208.1	2.0e-136	88.21	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
XP_004137737.1	2.2e-135	83.04	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]
KAA0044147.1	2.8e-135	87.07	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_023527474.1	1.2e-133	87.45	steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg033351 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QDF6	5.8e-99	66.29	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q38944	1.9e-97	63.50	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q5K2N1	1.1e-81	56.23	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
Q68FF9	2.6e-35	39.00	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
Q28891	3.5e-35	39.08	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]

BLAST of Spg033351 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B6E3	7.3e-137	83.04	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A0A0LAB3	1.0e-135	83.04	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A5D3DNY2	1.4e-135	87.07	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1CX04	4.9e-133	86.31	Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE...	[more]
A0A6J1J7F2	1.9e-132	86.31	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]

BLAST of Spg033351 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	1.3e-98	63.50	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	2.6e-14	34.82	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	3.5e-14	39.13	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.1e-12	37.39	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.1e-12	37.39	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.120.1630		coord: 180..366 e-value: 3.4E-12 score: 48.5
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 107..367
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 216..367 e-value: 2.3E-46 score: 157.5
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 258..341 score: 26.556953
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 107..367

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033351.1	Spg033351.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors