Spg033325 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033325 vs. NCBI nr
Match: XP_038905635.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 682/714 (95.52%), Postives = 703/714 (98.46%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F KN RRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGKITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMAV+AR
Sbjct: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVSAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSRQLQALKSEDR+KILLDVADALEDNENMIMAEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 301 ESSRQLQALKSEDRKKILLDVADALEDNENMIMAENNADIKAAEEAGYEKPLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI LLANSIR LADMEEPIGRILKR ELASDL+LENVSCSLGV+LIVFESRPDALVQIA
Sbjct: 361 GKIKLLANSIRKLADMEEPIGRILKRSELASDLVLENVSCSLGVLLIVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA++EMAK+IVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADMEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLI+LVTELRKEGVTL+GGKR SELLKIKEA SFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLIKLVTELRKEGVTLYGGKRASELLKIKEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHIHQHGSSHTDCI+TEDQEVAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITEDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTSRIHARGPVGVEGLLTTRWIL+G+GQ+VNGDKGVAY HKDL+L+N
Sbjct: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQIVNGDKGVAYIHKDLTLKN 714

BLAST of Spg033325 vs. NCBI nr
Match: XP_038905634.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1318.9 bits (3412), Expect = 0.0e+00
Identity = 678/714 (94.96%), Postives = 702/714 (98.32%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +DP+R+F K  +RLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 4   IDPTRIFIKTVKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 64  GLGRQRLRYRKLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 124 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGKITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMAV+AR
Sbjct: 244 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVSAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSRQLQALKSEDR+KILLDVADALEDNENMIMAEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 304 ESSRQLQALKSEDRKKILLDVADALEDNENMIMAENNADIKAAEEAGYEKPLISRLALKP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI LLANSIR LADMEEPIGRILKR ELASDL+LENVSCSLGV+LIVFESRPDALVQIA
Sbjct: 364 GKIKLLANSIRKLADMEEPIGRILKRSELASDLVLENVSCSLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLDDV
Sbjct: 424 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA++EMAK+IVSDSKVDYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADMEMAKRIVSDSKVDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLI+LVTELRKEGVTL+GGKR SELLKIKEA SFHHEYNSLTCT
Sbjct: 544 NAMETLLVHKDLASNGGLIKLVTELRKEGVTLYGGKRASELLKIKEASSFHHEYNSLTCT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHIHQHGSSHTDCI+TEDQEVAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 604 VEIVDDVFAAIDHIHQHGSSHTDCIITEDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTSRIHARGPVGVEGLLTTRWIL+G+GQ+VNGDKGVAY HKDL+L+N
Sbjct: 664 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGSGQIVNGDKGVAYIHKDLTLKN 717

BLAST of Spg033325 vs. NCBI nr
Match: XP_011651883.1 (delta-1-pyrroline-5-carboxylate synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 663/714 (92.86%), Postives = 698/714 (97.76%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F KN RR+VVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNARRIVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVN+LLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFSDTGFRQQLSETVNALLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFG KSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGQITFGAKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMA++AR
Sbjct: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAISAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKIL+DVADALEDNENMI+AEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 301 ESSRRLQALKSEDRRKILMDVADALEDNENMILAENSADIKAAEEAGYEKPLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GK+ LLANS+R LADMEEPIGRILKR ELA DL+LE+VSCSLGV+L+VFESRPDALVQIA
Sbjct: 361 GKVKLLANSVRKLADMEEPIGRILKRSELARDLVLESVSCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLD+V
Sbjct: 421 SLAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDNV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSD+KVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVH+DLA+NGGLI+L+TELRKEGVTL+GGKR SELLKI EA SFHHEYNSLTCT
Sbjct: 541 NAMETLLVHEDLANNGGLIKLITELRKEGVTLYGGKRASELLKINEAHSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHI++HGSSHTDCI+TEDQ+VAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHINEHGSSHTDCIITEDQKVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTSRIHARGPVGVEGLLTTRWIL+G GQVVNGD GVAY HKDL+L+N
Sbjct: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVNGDNGVAYIHKDLALKN 714

BLAST of Spg033325 vs. NCBI nr
Match: XP_004137758.1 (delta-1-pyrroline-5-carboxylate synthase isoform X1 [Cucumis sativus] >KGN59076.2 hypothetical protein Csa_002101 [Cucumis sativus])

HSP 1 Score: 1292.3 bits (3343), Expect = 0.0e+00
Identity = 659/714 (92.30%), Postives = 697/714 (97.62%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +DP+R+F +  +RLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 4   IDPTRIFIRKVKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 64  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVN+LLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 124 TDSVFSDTGFRQQLSETVNALLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFG KSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGQITFGAKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMA++AR
Sbjct: 244 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAISAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKIL+DVADALEDNENMI+AEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 304 ESSRRLQALKSEDRRKILMDVADALEDNENMILAENSADIKAAEEAGYEKPLISRLALKP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GK+ LLANS+R LADMEEPIGRILKR ELA DL+LE+VSCSLGV+L+VFESRPDALVQIA
Sbjct: 364 GKVKLLANSVRKLADMEEPIGRILKRSELARDLVLESVSCSLGVLLVVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLD+V
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDNV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSD+KVDYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKVDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVH+DLA+NGGLI+L+TELRKEGVTL+GGKR SELLKI EA SFHHEYNSLTCT
Sbjct: 544 NAMETLLVHEDLANNGGLIKLITELRKEGVTLYGGKRASELLKINEAHSFHHEYNSLTCT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHI++HGSSHTDCI+TEDQ+VAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 604 VEIVDDVFAAIDHINEHGSSHTDCIITEDQKVAEIFLNQVDSAAVFHNASTRFCDGARFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTSRIHARGPVGVEGLLTTRWIL+G GQVVNGD GVAY HKDL+L+N
Sbjct: 664 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVNGDNGVAYIHKDLALKN 717

BLAST of Spg033325 vs. NCBI nr
Match: XP_023529106.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1291.6 bits (3341), Expect = 0.0e+00
Identity = 662/714 (92.72%), Postives = 694/714 (97.20%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F KN RRLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVF DTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAV ASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMAV AR
Sbjct: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKILLDVA+AL+DNE+MIMAEN ADIKAAE+AGYEKSLISRLALKP
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           G+I LLANSIR +ADMEEPIGRILKR+E+ASDLILEN +CSLGV+L+VFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPDTVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGL+ELV ELRKEGVTL+GG+R S+LL+I EA SFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHIHQHGSSHTDCI+T+DQEVAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVG+STSRIHARGPVGVEGLLTTRWIL+GNGQVVNGDKGV Y HKDL+ +N
Sbjct: 661 LGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQVVNGDKGVTYIHKDLTQKN 714

BLAST of Spg033325 vs. ExPASy Swiss-Prot
Match: O65361 (Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=3544 GN=P5CS PE=2 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 554/714 (77.59%), Postives = 627/714 (87.82%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MD +R F K+ +R+VVKVGTAVVTR DGRLALGRLG+LCEQLKELNS GYEVILVTSGAV
Sbjct: 1   MDATRAFVKDVKRVVVKVGTAVVTRSDGRLALGRLGSLCEQLKELNSDGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
             GRQRLR+R+L NSSFADLQ PQ + DGKACAAVGQ+ LMALYDT+FSQLD+T++QLLV
Sbjct: 61  SAGRQRLRFRKLVNSSFADLQKPQVELDGKACAAVGQNGLMALYDTLFSQLDLTAAQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR QL+ETV  LLDL+V+P+ NENDAVSTRK PYEDSSGIFWDNDSLA LL
Sbjct: 121 TDNDFRDPSFRTQLTETVYQLLDLKVVPVLNENDAVSTRKAPYEDSSGIFWDNDSLAALL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDV+GLY+GPPSDP SKLI T+VKEKH GEITFGDKSR+GRGGMTAKV
Sbjct: 181 ALELKADLLILLSDVDGLYNGPPSDPRSKLISTYVKEKHQGEITFGDKSRLGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAVYA+ AGIPV+I SG ATDNIIKV+ G+  GTLFHKDAHLW   KE   R+MAVAAR
Sbjct: 241 KAAVYAAYAGIPVIIASGKATDNIIKVIDGQCVGTLFHKDAHLWVQVKETGVRDMAVAAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQA+ SE+R+KILLD+ADALE NE  I+AEN AD+ AA+ AGY++SL++RLA+ P
Sbjct: 301 ESSRRLQAVSSEERKKILLDIADALEANEEKILAENEADVAAAQYAGYDRSLVARLAMNP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
            KI  LA SIR+LADMEEPIGRILKR E+A  LILE  SC LGV+LIVFESRPDALVQIA
Sbjct: 361 DKISSLAKSIRVLADMEEPIGRILKRTEIADGLILEKTSCPLGVLLIVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEA+RSNA LHKVITSAIPD VGEKLIGL+TSR+EIPDLLKLDDV
Sbjct: 421 SLAIRSGNGLLLKGGKEAKRSNAILHKVITSAIPDKVGEKLIGLVTSRDEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKEST+IPVLGH+DGICHVYVDKSAN++MAK+IV D+K DYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSEL--LKIKEARSFHHEYNSLT 600
           NAMETLLVHKDLA NGGL +L+ +LR EGVT+FGG R+  L    I+  ++F+ EY+S  
Sbjct: 541 NAMETLLVHKDLAENGGLNDLIVDLRTEGVTMFGGPRIDALQEFNIQATQTFNREYSSPA 600

Query: 601 CTVEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGAR 660
           CTVEIVDDV+AAI+HI+ HGS+HTDCI+ ED +VAE FL  VDSAAV HNASTRFCDG R
Sbjct: 601 CTVEIVDDVYAAIEHINHHGSAHTDCIIAEDHKVAETFLQLVDSAAVLHNASTRFCDGFR 660

Query: 661 FGLGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSL 713
           FGLGAEVGISTSRIHARGPVGVEGLLTTRW+LKG+GQVV+GDKGV YTHKDL L
Sbjct: 661 FGLGAEVGISTSRIHARGPVGVEGLLTTRWVLKGSGQVVHGDKGVVYTHKDLPL 714

BLAST of Spg033325 vs. ExPASy Swiss-Prot
Match: O04015 (Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 552/711 (77.64%), Postives = 625/711 (87.90%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D +R F K  +R+++KVGTAVVTR DGRLALGRLGALCEQ+ ELNS+G+EVILVTSGAV
Sbjct: 4   VDSTRAFVKGVKRVIIKVGTAVVTRADGRLALGRLGALCEQIHELNSQGFEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYR+L NSSFADLQ PQ + DGKACAAVGQ+ L+ALYDT+FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLVNSSFADLQKPQIELDGKACAAVGQNGLLALYDTLFSQLDVTSAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL+ETV SLL+L+VIPIFNENDAVSTRK PYED+SGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPEFRKQLTETVESLLNLKVIPIFNENDAVSTRKAPYEDASGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDP SKLI T++KE   G ITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPQSKLIHTYIKEMFEGLITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAVYA+ AGIPVVITSG+AT+NIIKVLQGE  GTLFH+DA  W    +V AR+MAVAAR
Sbjct: 244 KAAVYAAHAGIPVVITSGYATNNIIKVLQGERIGTLFHRDAQKWAPVGDVGARDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQA+  +DR KILLDVADALE NE +I  EN AD+ AA+ AGYEKSLISRLALK 
Sbjct: 304 ESSRRLQAMSPQDRSKILLDVADALEANEKLIRIENEADLAAAQQAGYEKSLISRLALKS 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA SIR+LA+MEEPIG +LKR E+   L+LE  S  LGV+LI+FESRPDALVQIA
Sbjct: 364 GKISSLAKSIRVLANMEEPIGHVLKRTEITDGLVLEKTSSPLGVLLIIFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGL+LKGGKEA+RSNA LHKVITSAIP+ VG +LIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLVLKGGKEAKRSNAILHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGH+DGICHVYVDKSAN++MAK++V D+K DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  NG L EL+ EL+ +GV + GG R S LL I EARS HHEY+SL CT
Sbjct: 544 NAMETLLVHKDLVQNGCLDELIVELQIKGVVIHGGPRASSLLHIPEARSLHHEYSSLACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           +EIVDDV+AAIDHIH+HGS+HTD I+TED EVAEIFL +VDS++V HNASTRF DGARFG
Sbjct: 604 IEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLS 712
           LGAEVGISTSRIHARGPVGVEGLLTTRWI +G+GQVV+GDKG+ YTHKDL+
Sbjct: 664 LGAEVGISTSRIHARGPVGVEGLLTTRWIARGSGQVVDGDKGIVYTHKDLT 714

BLAST of Spg033325 vs. ExPASy Swiss-Prot
Match: Q96480 (Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2 PE=2 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 544/713 (76.30%), Postives = 627/713 (87.94%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D +R F KN +RL+VKVGTAVVTR DGRLALGRLGALCEQL+ELNS+GYEVILVTSGAV
Sbjct: 4   VDSTRAFVKNVKRLIVKVGTAVVTRADGRLALGRLGALCEQLQELNSQGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYR+L NSSF DLQ PQ + DGKACAAVGQ+ LMALYD++FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLLNSSFLDLQKPQTELDGKACAAVGQNGLMALYDSLFSQLDVTSAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL++TVNSLL L+VIPIFNENDA+STR+ PYEDSSGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPDFRRQLNDTVNSLLSLKVIPIFNENDAISTRRAPYEDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDV+GLYSGPP DP+SKLI T++KE H   ITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVDGLYSGPPRDPDSKLIYTYIKEIHERVITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AA+YA+ AGIPVVITSGFATDNIIKVL GE  GTLFH DA+ W    E  AREMAVAAR
Sbjct: 244 KAAMYAAYAGIPVVITSGFATDNIIKVLHGERIGTLFHCDANKWASIGETDAREMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
             SR+LQAL S++R KIL D+ADALE NE  I+AEN AD+ AA+ AGYEKSLISRLAL P
Sbjct: 304 ACSRRLQALSSQERSKILQDIADALEANEKAILAENEADVVAAQQAGYEKSLISRLALNP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LANS+R+L++M+EP+G  LKR E+A   ILE  S  LGV+LI+FESRPDALVQIA
Sbjct: 364 GKISSLANSVRVLSNMDEPLGHTLKRTEIADGFILEKSSSPLGVVLIIFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LA+RSGNGL+LKGGKEA+RSNA LHKVITSAIP +VGE+LIGL+TSREEIP+LLKLDDV
Sbjct: 424 SLAVRSGNGLMLKGGKEAKRSNAILHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYVDKSA+++MAK+I  D+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLA NGGL +L+ EL+ +GV+L+GG + S LL I EAR+F HEY+SL CT
Sbjct: 544 NAMETLLVHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMIPEARTFRHEYSSLACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE+V+DV+AAIDHIHQHGS+HTD I+TEDQEVAE+FL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVAEVFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVGIST RIHARGPVGVEGLLTT+W+ +G+GQ+V+GDK + Y+HKDL+ Q
Sbjct: 664 LGAEVGISTGRIHARGPVGVEGLLTTKWLARGSGQIVDGDKSIVYSHKDLTQQ 716

BLAST of Spg033325 vs. ExPASy Swiss-Prot
Match: O04226 (Delta-1-pyrroline-5-carboxylate synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=P5CS1 PE=2 SV=2)

HSP 1 Score: 1058.5 bits (2736), Expect = 3.3e-308
Identity = 537/713 (75.32%), Postives = 617/713 (86.54%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +DPSR F ++ +R+++KVGTAVV+R DGRLALGR+GALCEQ+KELNS GYEVILVTSGAV
Sbjct: 4   VDPSRSFVRDVKRVIIKVGTAVVSRQDGRLALGRVGALCEQVKELNSLGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYR+L NSSFADLQ PQ + DGKACAAVGQS LMALYD +F+QLDV+SSQLLV
Sbjct: 64  GVGRQRLRYRKLVNSSFADLQKPQMELDGKACAAVGQSGLMALYDMLFNQLDVSSSQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDS F +  FR+QL+ETV SLLDL+VIPIFNENDA+STRK PYEDSSGIFWDNDSLAGLL
Sbjct: 124 TDSDFENPKFREQLTETVESLLDLKVIPIFNENDAISTRKAPYEDSSGIFWDNDSLAGLL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDV+GLYSGPPS+P+SK+I T++KEKH  EITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVDGLYSGPPSEPSSKIIHTYIKEKHQQEITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV AS +G PVVITSGF   +I+KVL GE  GTLFHK+A+LW   K+VS REMAVAAR
Sbjct: 244 KAAVLASNSGTPVVITSGFENRSILKVLHGEKIGTLFHKNANLWESSKDVSTREMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           + SR LQ L SE+R+KILLDVADALE NE++I +EN AD+ AA+ AGYEK L++RL +KP
Sbjct: 304 DCSRHLQNLSSEERKKILLDVADALEANEDLIRSENEADVAAAQVAGYEKPLVARLTIKP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA SIR LA+ME+PI +ILK+ E+A DL+LE  SC LGV+LIVFESRPDALVQIA
Sbjct: 364 GKIASLAKSIRTLANMEDPINQILKKTEVADDLVLEKTSCPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEA RSN  LHKVIT AIP  VGEKLIGL+T+R+EI DLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEAIRSNTILHKVITDAIPRNVGEKLIGLVTTRDEIADLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVY+DKSA+++MAK IV D+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  + GL +++  L+ EGV ++GG    + L   +A SFHHEY+S+ CT
Sbjct: 544 NAMETLLVHKDLMKSPGLDDILVALKTEGVNIYGGPIAHKALGFPKAVSFHHEYSSMACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE VDDV +AIDHIH++GS+HTDCIVT D +VAE FL RVDSAAVFHNASTRF DGARFG
Sbjct: 604 VEFVDDVQSAIDHIHRYGSAHTDCIVTTDDKVAETFLRRVDSAAVFHNASTRFSDGARFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVGIST RIHARGPVGVEGLLTTRWIL+G GQVVNGDK V YTHK L LQ
Sbjct: 664 LGAEVGISTGRIHARGPVGVEGLLTTRWILRGRGQVVNGDKDVVYTHKSLPLQ 716

BLAST of Spg033325 vs. ExPASy Swiss-Prot
Match: P54887 (Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5CSA PE=1 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 9.6e-308
Identity = 534/713 (74.89%), Postives = 618/713 (86.68%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR FA++ +R+VVKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPPSDPNSKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW    + +AR+MAVAAR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQAL SEDR+KILLD+ADALE N   I AEN  D+ +A++AG E+S+++RL + P
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA S+R LADME+PIGR+LK+ E+A  L+LE  S  LGV+LIVFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ + +MAK+IVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTL+GG R S++L I EARSF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE+V+DV+ AIDHIH+HGS+HTDCIVTED EVAE+FL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVG+ST RIHARGPVGVEGLLTTRWI++G GQVV+GD G+ YTH+D+ +Q
Sbjct: 664 LGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQ 716

BLAST of Spg033325 vs. ExPASy TrEMBL
Match: A0A0A0LAF9 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G733920 PE=3 SV=1)

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 663/714 (92.86%), Postives = 698/714 (97.76%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F KN RR+VVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNARRIVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVN+LLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFSDTGFRQQLSETVNALLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFG KSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGQITFGAKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMA++AR
Sbjct: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAISAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKIL+DVADALEDNENMI+AEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 301 ESSRRLQALKSEDRRKILMDVADALEDNENMILAENSADIKAAEEAGYEKPLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GK+ LLANS+R LADMEEPIGRILKR ELA DL+LE+VSCSLGV+L+VFESRPDALVQIA
Sbjct: 361 GKVKLLANSVRKLADMEEPIGRILKRSELARDLVLESVSCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLD+V
Sbjct: 421 SLAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDNV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSD+KVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVH+DLA+NGGLI+L+TELRKEGVTL+GGKR SELLKI EA SFHHEYNSLTCT
Sbjct: 541 NAMETLLVHEDLANNGGLIKLITELRKEGVTLYGGKRASELLKINEAHSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHI++HGSSHTDCI+TEDQ+VAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHINEHGSSHTDCIITEDQKVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTSRIHARGPVGVEGLLTTRWIL+G GQVVNGD GVAY HKDL+L+N
Sbjct: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILRGTGQVVNGDNGVAYIHKDLALKN 714

BLAST of Spg033325 vs. ExPASy TrEMBL
Match: A0A2D0UVX8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 658/714 (92.16%), Postives = 696/714 (97.48%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +DP+R+F +  +RLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 4   IDPTRIFIRKVKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLL 
Sbjct: 64  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLE 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFSDTGFRQQLSETVN+LLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 124 TDSVFSDTGFRQQLSETVNALLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLG+ITFG KSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGQITFGAKSRVGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMA++AR
Sbjct: 244 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAISAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKIL+DVADALEDNENMI+AEN ADIKAAE+AGYEK LISRLALKP
Sbjct: 304 ESSRRLQALKSEDRRKILMDVADALEDNENMILAENSADIKAAEEAGYEKPLISRLALKP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GK+ LLANS+R LADMEEPIGRILKR ELA DL+LE+VSCSLGV+L+VFESRPDALVQIA
Sbjct: 364 GKVKLLANSVRKLADMEEPIGRILKRSELARDLVLESVSCSLGVLLVVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGL+TSREEIPDLLKLD+V
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLVTSREEIPDLLKLDNV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSD+KVDYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKVDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVH+DLA+NGGLI+L+TELRKEGVTL+GGKR SELLKI EA SFHHEYNSLTCT
Sbjct: 544 NAMETLLVHEDLANNGGLIKLITELRKEGVTLYGGKRASELLKINEAHSFHHEYNSLTCT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHI++HGSSHTDCI+TEDQ+VAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 604 VEIVDDVFAAIDHINEHGSSHTDCIITEDQKVAEIFLNQVDSAAVFHNASTRFCDGARFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVGISTS IHARGPVGVEGLLTTRWIL+G GQVVNGD GVAYTHKDL+L+N
Sbjct: 664 LGAEVGISTSGIHARGPVGVEGLLTTRWILRGTGQVVNGDNGVAYTHKDLALKN 717

BLAST of Spg033325 vs. ExPASy TrEMBL
Match: A0A6J1F9I8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111442049 PE=3 SV=1)

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 661/714 (92.58%), Postives = 694/714 (97.20%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F KN RRLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVF DTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAV ASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMAV AR
Sbjct: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKILLDVA+AL+DNE+MIMAEN ADIKAAE+AGYEKSLISRLALKP
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           G+I LLANSIR +ADMEEPIGRILKR+E+ASDLILEN +CSLGV+L+VFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPDTVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGL+ELV ELRKEGVTL+GG+R S+LL+I EA SFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGERASQLLRISEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHIHQ+GSSHTDCI+T+DQEVAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVG+STSRIHARGPVGVEGLLTTRWIL+GNGQVVNGDKGV Y HKDL+ +N
Sbjct: 661 LGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQVVNGDKGVTYIHKDLTQKN 714

BLAST of Spg033325 vs. ExPASy TrEMBL
Match: A0A6J1CXK2 (Delta-1-pyrroline-5-carboxylate synthase OS=Momordica charantia OX=3673 GN=LOC111015124 PE=3 SV=1)

HSP 1 Score: 1286.9 bits (3329), Expect = 0.0e+00
Identity = 659/712 (92.56%), Postives = 690/712 (96.91%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F +N RRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTRNARRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVF D+GFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDSGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DPNSKLI+TF+KEKHLGEITFGDKSR+GRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPCDPNSKLIRTFIKEKHLGEITFGDKSRLGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAVYA+ AGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWT+ KE+SAR+MAV+AR
Sbjct: 241 NAAVYAASAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTVVKEISARDMAVSAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSRQLQALKSEDRRKILLDVADALEDNE+MI+AEN ADIKAAE+ GYEKSLISRLALKP
Sbjct: 301 ESSRQLQALKSEDRRKILLDVADALEDNEDMIIAENLADIKAAEEVGYEKSLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           G+IG LA+SIR LADMEEPIGRILKR+ELA DLILEN SCSLGV+LIVFESRPDALVQIA
Sbjct: 361 GRIGTLASSIRKLADMEEPIGRILKRKELADDLILENTSCSLGVLLIVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSD+K DYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDAKADYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNG LIELVTELRKEGVTL+GGKR S +LKIKEA SFHHEYNSL CT
Sbjct: 541 NAMETLLVHKDLASNGMLIELVTELRKEGVTLYGGKRASAILKIKEASSFHHEYNSLACT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVF+AIDHIH+HGS+HTDC+V EDQEVAEIFLN+VDSAAV HNASTRFCDGARFG
Sbjct: 601 VEIVDDVFSAIDHIHEHGSAHTDCVVAEDQEVAEIFLNQVDSAAVIHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSL 713
           LGAEVGIST RIHARGPVGVEGLLTTRWIL+G+GQVVNGDKGVAYTHKDL +
Sbjct: 661 LGAEVGISTGRIHARGPVGVEGLLTTRWILRGSGQVVNGDKGVAYTHKDLGV 712

BLAST of Spg033325 vs. ExPASy TrEMBL
Match: A0A6J1IX71 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111481470 PE=3 SV=1)

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 656/714 (91.88%), Postives = 693/714 (97.06%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSR+F +N RRLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTRNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+LANSSFADLQNPQG+FDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVF DTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
           NAAV ASCAGIPVVITSGFA DNIIKVLQGEMKGTLFHKDAHLWTL KEVSAREMAV AR
Sbjct: 241 NAAVCASCAGIPVVITSGFAADNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQALKSEDRRKILLDVA+AL+DNENMI+AEN ADIKAAE+AGYEKSLISRLALKP
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNENMIIAENCADIKAAEEAGYEKSLISRLALKP 360

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           G+I LLANSIR +ADMEEPIGRILKRRE+ASDLILEN +CSLGV+L+VFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRREIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEAR+SNAALHKVITSAIPDTVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA+IEMAK+IVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGL+ELV ELRK+GVTL+GG+R S+LL+I EA SFHHEYNS+TCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKDGVTLYGGQRASQLLRISEASSFHHEYNSVTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIVDDVFAAIDHIH++GSSHTDCI+T+DQEVAEIFLN+VDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHKYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQN 715
           LGAEVG+STSRIHARGPVGVEGLLTTRWIL+G+GQVVNGDKGV Y HKDL+ +N
Sbjct: 661 LGAEVGVSTSRIHARGPVGVEGLLTTRWILRGSGQVVNGDKGVTYIHKDLTQKN 714

BLAST of Spg033325 vs. TAIR 10
Match: AT2G39800.1 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1057.0 bits (2732), Expect = 6.8e-309
Identity = 534/713 (74.89%), Postives = 618/713 (86.68%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR FA++ +R+VVKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPPSDPNSKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW    + +AR+MAVAAR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQAL SEDR+KILLD+ADALE N   I AEN  D+ +A++AG E+S+++RL + P
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA S+R LADME+PIGR+LK+ E+A  L+LE  S  LGV+LIVFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ + +MAK+IVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTL+GG R S++L I EARSF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE+V+DV+ AIDHIH+HGS+HTDCIVTED EVAE+FL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVG+ST RIHARGPVGVEGLLTTRWI++G GQVV+GD G+ YTH+D+ +Q
Sbjct: 664 LGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQ 716

BLAST of Spg033325 vs. TAIR 10
Match: AT2G39800.4 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1057.0 bits (2732), Expect = 6.8e-309
Identity = 534/713 (74.89%), Postives = 618/713 (86.68%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR FA++ +R+VVKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPPSDPNSKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW    + +AR+MAVAAR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQAL SEDR+KILLD+ADALE N   I AEN  D+ +A++AG E+S+++RL + P
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA S+R LADME+PIGR+LK+ E+A  L+LE  S  LGV+LIVFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ + +MAK+IVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTL+GG R S++L I EARSF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE+V+DV+ AIDHIH+HGS+HTDCIVTED EVAE+FL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVG+ST RIHARGPVGVEGLLTTRWI++G GQVV+GD G+ YTH+D+ +Q
Sbjct: 664 LGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQ 716

BLAST of Spg033325 vs. TAIR 10
Match: AT3G55610.1 (delta 1-pyrroline-5-carboxylate synthase 2 )

HSP 1 Score: 1055.4 bits (2728), Expect = 2.0e-308
Identity = 533/710 (75.07%), Postives = 617/710 (86.90%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR FAK+ +R+VVKVGTAVVT   GRLALGRLGA+CEQL ELNS G+EVILV+SGAV
Sbjct: 4   IDRSRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT +Q+LV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDS F D  FR+QLSETV ++L +RVIP+FNENDA+STR+ PY+DS+GIFWDNDSLA LL
Sbjct: 124 TDSSFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           +LELKADLL+LLSDVEGLY+GPPSD  SKLI TF+KEKH  EITFG+KS++GRGGMTAKV
Sbjct: 184 SLELKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV A+  G+PV+ITSG+A +NI KVL+G   GTLFH+DAHLW    + ++R+MAVAAR
Sbjct: 244 KAAVNAAYGGVPVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQAL SEDR++IL D+A+ALE NE  I AEN  D+ AA++AGYE+SL++RL +KP
Sbjct: 304 ESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA S+R LA+ME+PIGR+LK+ ++A DLILE  S  +GV+LIVFESRPDALVQIA
Sbjct: 364 GKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYVDKS  ++MAK+IVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  NG L +L+  L+ +GVTL+GG R S  L I E +SFHHEY+S  CT
Sbjct: 544 NAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VEIV+DV+ AIDHIHQHGS+HTDCIVTED EVAEIFL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDL 711
           LGAEVGISTSRIHARGPVGVEGLLTTRWI++G GQVV+GD G+ YTHKDL
Sbjct: 664 LGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHKDL 713

BLAST of Spg033325 vs. TAIR 10
Match: AT2G39800.3 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 1045.8 bits (2703), Expect = 1.6e-305
Identity = 532/713 (74.61%), Postives = 615/713 (86.26%), Query Frame = 0

Query: 1   MDPSRLFAKNTRRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR FA++ +R+VVKVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRRLANSSFADLQNPQGDFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ + DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPPSDPNSKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLDKEVSAREMAVAAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW    + +AR+MAVAAR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADIKAAEDAGYEKSLISRLALKP 360
           ESSR+LQAL SEDR+KILLD+ADALE N   I AEN  D+ +A++AG E+S+++RL + P
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 GKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSCSLGVILIVFESRPDALVQIA 420
           GKI  LA S+R LADME+PIGR+LK+ E+A  L+LE  S  LGV+LIVFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEKLIGLITSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG KLIGL+TSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSANIEMAKQIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVL   DGICHVYVDK+ + +MAK+IVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVL---DGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSELLKIKEARSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTL+GG R S++L I EARSF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRVDSAAVFHNASTRFCDGARFG 660
           VE+V+DV+ AIDHIH+HGS+HTDCIVTED EVAE+FL +VDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

Query: 661 LGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGDKGVAYTHKDLSLQ 714
           LGAEVG+ST RIHARGPVGVEGLLTTRWI++G GQVV+GD G+ YTH+D+ +Q
Sbjct: 664 LGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGDNGIVYTHQDIPIQ 713

BLAST of Spg033325 vs. TAIR 10
Match: AT2G39800.2 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 908.3 bits (2346), Expect = 3.9e-264
Identity = 456/613 (74.39%), Postives = 531/613 (86.62%), Query Frame = 0

Query: 101 MALYDTMFSQLDVTSSQLLVTDSVFSDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRK 160
           MA Y+TMF QLDVT++QLLV DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+
Sbjct: 1   MAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRR 60

Query: 161 TPYEDSSGIFWDNDSLAGLLALELKADLLVLLSDVEGLYSGPPSDPNSKLIQTFVKEKHL 220
            PY+DSSGIFWDNDSLA LLALELKADLL+LLSDVEGLY+GPPSDPNSKLI TFVKEKH 
Sbjct: 61  APYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQ 120

Query: 221 GEITFGDKSRVGRGGMTAKVNAAVYASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKD 280
            EITFGDKSR+GRGGMTAKV AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+D
Sbjct: 121 DEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQD 180

Query: 281 AHLWTLDKEVSAREMAVAARESSRQLQALKSEDRRKILLDVADALEDNENMIMAENYADI 340
           A LW    + +AR+MAVAARESSR+LQAL SEDR+KILLD+ADALE N   I AEN  D+
Sbjct: 181 ARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDV 240

Query: 341 KAAEDAGYEKSLISRLALKPGKIGLLANSIRILADMEEPIGRILKRRELASDLILENVSC 400
            +A++AG E+S+++RL + PGKI  LA S+R LADME+PIGR+LK+ E+A  L+LE  S 
Sbjct: 241 ASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSS 300

Query: 401 SLGVILIVFESRPDALVQIAALAIRSGNGLLLKGGKEARRSNAALHKVITSAIPDTVGEK 460
            LGV+LIVFESRPDALVQIA+LAIRSGNGLLLKGGKEARRSNA LHKVIT AIP+TVG K
Sbjct: 301 PLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGK 360

Query: 461 LIGLITSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA 520
           LIGL+TSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ 
Sbjct: 361 LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKAC 420

Query: 521 NIEMAKQIVSDSKVDYPAACNAMETLLVHKDLASNGGLIELVTELRKEGVTLFGGKRVSE 580
           + +MAK+IVSD+K+DYPAACNAMETLLVHKDL  N  L EL+  L+  GVTL+GG R S+
Sbjct: 421 DTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASK 480

Query: 581 LLKIKEARSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIVTEDQEVAEIFLNRV 640
           +L I EARSF+HEY +  CTVE+V+DV+ AIDHIH+HGS+HTDCIVTED EVAE+FL +V
Sbjct: 481 ILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQV 540

Query: 641 DSAAVFHNASTRFCDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWILKGNGQVVNGD 700
           DSAAVFHNASTRF DG RFGLGAEVG+ST RIHARGPVGVEGLLTTRWI++G GQVV+GD
Sbjct: 541 DSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGD 600

Query: 701 KGVAYTHKDLSLQ 714
            G+ YTH+D+ +Q
Sbjct: 601 NGIVYTHQDIPIQ 613