Spg033260 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033260 vs. NCBI nr
Match: XP_023540054.1 (serine/threonine-protein kinase ATG1c-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 658/774 (85.01%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTG TRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGGTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRLHDI+E                                       
Sbjct: 61  EIFILKRINHPNIIRLHDIVE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AK FLQQLAAGLK+LRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAIAKRFLQQLAAGLKVLRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS DNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSGDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQ VTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQFVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFLCANQPDEL  SKRSSRLVDGFPFSESD A +MEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFNHPFLCANQPDELLGSKRSSRLVDGFPFSESDHAGSMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDR TRD+TSRYSS+PDK E    +
Sbjct: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRGTRDSTSRYSSLPDKREITNPL 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDD-PSS 480
            DT V+ GRN+L DRSL+  LRSANPQS+NTR R  DSLELIDQDYVLVSGP MDD  SS
Sbjct: 421 LDTRVQ-GRNILSDRSLNAGLRSANPQSVNTRPRVADSLELIDQDYVLVSGPSMDDYASS 480

Query: 481 SLASASKPI--PCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMD 540
           SLASASKPI   CK++S  Q SVSLGAA +AP+PII +A SSSSRMGSLGSQSSAPGS+D
Sbjct: 481 SLASASKPILSSCKSQSPTQPSVSLGAALTAPMPIIRKAASSSSRMGSLGSQSSAPGSLD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           +ED LEQPSANCT RIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 MEDTLEQPSANCTARIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAESRRSL KKQGSSLGKGS L+V S QPVDI++QVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAESRRSLRKKQGSSLGKGSPLMVDSLQPVDISTQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAA LYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAGLYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSY+DILSNRQGYLRSQRLALLKCENQQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYVDILSNRQGYLRSQRLALLKCENQQSPP 726

BLAST of Spg033260 vs. NCBI nr
Match: XP_022934901.1 (serine/threonine-protein kinase ATG1c-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 658/774 (85.01%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTG TRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGGTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILKKINHPNIIRLHDI+E                                       
Sbjct: 61  EIFILKKINHPNIIRLHDIVE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AK FLQQLAAGLK+LRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAIAKRFLQQLAAGLKVLRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS DNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSGDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQ VTGRTPFTGNNQIQLLQNIVKST+LHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQFVTGRTPFTGNNQIQLLQNIVKSTKLHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFLCANQPDEL  SKRSSRLVDGFPFSESD A +MEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFNHPFLCANQPDELLGSKRSSRLVDGFPFSESDHAGSMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDR TRD+TSRYSS+PDK E    +
Sbjct: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRGTRDSTSRYSSLPDKREITNPL 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDD-PSS 480
            DT V+ GRN+L DRSL+  LRSANPQS+NTR R  DSLELIDQDYVLVSGP MDD  SS
Sbjct: 421 LDTRVQ-GRNILSDRSLNAGLRSANPQSVNTRPRVADSLELIDQDYVLVSGPSMDDYASS 480

Query: 481 SLASASKPI--PCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMD 540
           SLASASKPI   CK++S  Q SVSLGAA +AP+PII +A SSSSRMGSLGSQSSAPGS+D
Sbjct: 481 SLASASKPILSSCKSQSPTQPSVSLGAALTAPMPIIRKAASSSSRMGSLGSQSSAPGSLD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           +ED LEQPSANCT RIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 MEDTLEQPSANCTARIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAESRRSL KKQGSSLGKGS L+V S QPVDI++QVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAESRRSLRKKQGSSLGKGSPLMVDSLQPVDISTQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAA LYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAGLYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSY+DILSNRQGYLRSQRLALLKCENQQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYVDILSNRQGYLRSQRLALLKCENQQSPP 726

BLAST of Spg033260 vs. NCBI nr
Match: XP_022935209.1 (serine/threonine-protein kinase ATG1c-like [Cucurbita moschata])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 648/764 (84.82%), Postives = 679/764 (88.87%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISM+RLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMTRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL+DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLYDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYC+GGDLSFYIQQRHGRIPEA AKHFLQQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCKGGDLSFYIQQRHGRIPEAIAKHFLQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLS SDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSASDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPF+CANQPDE  RSKRSSRL+D FPFSESDPASNMEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFKHPFVCANQPDESLRSKRSSRLLDEFPFSESDPASNMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFV RRSSMKSTYGFSPDKKVDR TRDTTSRY S+PDKMES TL+
Sbjct: 361 PFSLDDDSSGPEGSPSFV-RRSSMKSTYGFSPDKKVDRGTRDTTSRYGSIPDKMESTTLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
            DT V+L R+V  DRSL+GA RSAN  S+N R R VDS +LIDQDYV+VSGPPMDD SS+
Sbjct: 421 LDTRVQLERDVHSDRSLNGASRSANTLSVNNRPRVVDSPDLIDQDYVIVSGPPMDDTSST 480

Query: 481 LASASKPIPC-KTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMDIE 540
           LASASKPIP  K++S PQ+S+ LG A +AP+PIIGRAT+SS RMGSLGSQSSAPGSMDIE
Sbjct: 481 LASASKPIPSRKSQSPPQSSIRLGTAVTAPMPIIGRATTSSYRMGSLGSQSSAPGSMDIE 540

Query: 541 DALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT 600
           D LEQPSANC  R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT
Sbjct: 541 DTLEQPSANCRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT 600

Query: 601 QAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYA 660
           QAASALEGSPGQGSAE RRSL KKQGSSLGK S LIV+SHQPVDI+SQVEREFLREVEYA
Sbjct: 601 QAASALEGSPGQGSAEFRRSLSKKQGSSLGKESHLIVNSHQPVDISSQVEREFLREVEYA 660

Query: 661 EELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLLV 720
           EELAK+VEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEME+AAALYSK+AQLLVFLLV
Sbjct: 661 EELAKIVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMETAAALYSKAAQLLVFLLV 716

Query: 721 EAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLK 764
           EAPSLILNPPFSLTNSDRYRLRSYIDIL+NRQGYLRSQRLALLK
Sbjct: 721 EAPSLILNPPFSLTNSDRYRLRSYIDILNNRQGYLRSQRLALLK 716

BLAST of Spg033260 vs. NCBI nr
Match: XP_038905361.1 (serine/threonine-protein kinase ATG1c-like [Benincasa hispida])

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 647/771 (83.92%), Postives = 681/771 (88.33%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLFDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYC+GGDLSFYIQQRHGRIPEA AK+F+QQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCKGGDLSFYIQQRHGRIPEAIAKYFMQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS DNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSGDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTEL FPSD NDLSFDCKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELQFPSDINDLSFDCKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPFLCANQPDE  RSKRSSRL+D FPFSESDPAS MEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFKHPFLCANQPDESLRSKRSSRLLDEFPFSESDPASKMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPS +R RSS+KSTYGFSPDKKV+R TRDTTSRYSS+PDKMESATL+
Sbjct: 361 PFSLDDDSSGPEGSPSSIRNRSSVKSTYGFSPDKKVNRGTRDTTSRYSSIPDKMESATLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
           SDT V+L R+V  DRSL+  LRS N +S+N R R VDSLE IDQDYV+VSGPPMDD SS+
Sbjct: 421 SDTRVQLERDVHSDRSLNDVLRSVNRRSVNNRPRVVDSLEPIDQDYVIVSGPPMDDASST 480

Query: 481 LASASKPIPCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMDIED 540
           LAS SKPIP +   SPQTS+SLG+A +AP+PIIGRAT SS RMGSLGSQSSAPGSMD+ED
Sbjct: 481 LASGSKPIPSRKSQSPQTSISLGSALTAPMPIIGRAT-SSYRMGSLGSQSSAPGSMDMED 540

Query: 541 ALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQ 600
            LEQPSAN   R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQ
Sbjct: 541 TLEQPSANSRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQ 600

Query: 601 AASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYAE 660
           AASALEGSPGQGSAE RRSL KKQGSSLGK S+L V+SHQPVDI+SQ+EREFLREVEYAE
Sbjct: 601 AASALEGSPGQGSAEYRRSLSKKQGSSLGKESRLSVNSHQPVDISSQIEREFLREVEYAE 660

Query: 661 ELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLLVE 720
           ELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSK+AQLLVFLLVE
Sbjct: 661 ELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKAAQLLVFLLVE 720

Query: 721 APSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           APSLILNPPFSLTNSDR+RL++YIDIL NRQGYLRSQRLALLKCE+QQSPP
Sbjct: 721 APSLILNPPFSLTNSDRFRLQNYIDILYNRQGYLRSQRLALLKCESQQSPP 723

BLAST of Spg033260 vs. NCBI nr
Match: XP_008442508.1 (PREDICTED: serine/threonine-protein kinase ATG1c [Cucumis melo] >KAA0044119.1 serine/threonine-protein kinase ATG1c [Cucumis melo var. makuwa] >TYK25019.1 serine/threonine-protein kinase ATG1c [Cucumis melo var. makuwa])

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 653/774 (84.37%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLFDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AKHFLQQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAVAKHFLQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS++NSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSEENSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLS DCKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSNDCKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPFLCANQ DE  RSKRSSRL+D FPFSESDPAS MEENSQD+YL
Sbjct: 301 RRNPVERLTFEEFFKHPFLCANQADESLRSKRSSRLLDEFPFSESDPASKMEENSQDEYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPS +RR SSMKSTYGFSPDKKVDR TR TTSR+SSVPDKMESATL+
Sbjct: 361 PFSLDDDSSGPEGSPS-LRRMSSMKSTYGFSPDKKVDRGTRGTTSRHSSVPDKMESATLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
           SDT V+L R+V  DRSL+  LRS NP+S+N R R VDS+E IDQDYV+VSGPPMDDPSS+
Sbjct: 421 SDTRVQLERDVHSDRSLNSVLRSVNPRSVNNRPRVVDSVESIDQDYVIVSGPPMDDPSST 480

Query: 481 LASASKPIPC-KTRSSPQTSVSLGAASSAPVPIIGRA--TSSSSRMGSLGSQSSAPGSMD 540
           L SAS+PIP  K++S PQTS+SLG A +AP+PIIGRA  T+SS RMGSLGSQSSAPGSMD
Sbjct: 481 LPSASRPIPSHKSQSPPQTSISLGTALTAPMPIIGRATTTTSSYRMGSLGSQSSAPGSMD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           IED LEQPSANC  R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 IEDTLEQPSANCRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAE RRSL KKQGS LGK S+LIVS HQPVDI+SQVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAEFRRSLSKKQGSPLGKESRLIVSPHQPVDISSQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPG TEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGKTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSYI+IL NRQGYLRSQRLALLKCE+QQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIEILYNRQGYLRSQRLALLKCESQQSPP 726

BLAST of Spg033260 vs. ExPASy Swiss-Prot
Match: F4IRW0 (Serine/threonine-protein kinase ATG1c OS=Arabidopsis thaliana OX=3702 GN=ATG1C PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 9.6e-229
Identity = 455/772 (58.94%), Postives = 555/772 (71.89%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MA  TG  RVV DYLVGRQIGSGSFSVVW ARHRV GTEVAIKEI+M RL+KKLQ+SLMS
Sbjct: 1   MAQFTG--RVVGDYLVGRQIGSGSFSVVWEARHRVDGTEVAIKEIAMDRLNKKLQESLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFIL++INHPNIIRL D+                                         
Sbjct: 61  EIFILRRINHPNIIRLIDM----------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                 I+ PGK+HLVLEYC+GGDLS Y+ QRHG +PEA AKHF+QQLAAGL++LRDNN+
Sbjct: 121 ------IKSPGKVHLVLEYCKGGDLSVYV-QRHGIVPEATAKHFMQQLAAGLQVLRDNNI 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLST+++++ LKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTNENDADLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGN+QIQLLQNI++STELHFP D  DLS DC DLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNSQIQLLQNIIRSTELHFPGDCRDLSLDCIDLCQKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFL   Q  + SRS+   R +DGF  S S P+ NMEE+SQ+D L
Sbjct: 301 RRNPVERLTFEEFFNHPFLSDRQSYDFSRSRLGLRTMDGFLSSGSSPSRNMEESSQEDCL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRT--------RDTTSRYSSVPD 420
           PF LDDDSSGPEGSPS++++ SSMKS+ G   D +++R+          + TS YSS   
Sbjct: 361 PFLLDDDSSGPEGSPSYLKKTSSMKSSSGIKVDTRIERKEVESSPLKHTELTSGYSSFNQ 420

Query: 421 KMESATLVSDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGP 480
           K+E+     +T +   R     R+        + +S+    R  DS + +DQD+VLVSGP
Sbjct: 421 KVENDRFRFETQINSDR-----RNRREPTGLTDSRSLIAPGRVDDSQDSMDQDFVLVSGP 480

Query: 481 PMDDPSSSLASASKP--IPCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQS 540
           P+D PSSS +S+SKP   P K++S P    +   +S+AP+PIIG  ++S  + GSL SQ 
Sbjct: 481 PVDMPSSS-SSSSKPYNFPFKSQSPPVELFNRSISSTAPMPIIGATSNSIGQFGSLDSQY 540

Query: 541 SAP----GSMDIEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVI 600
           SAP    GS+D+ DA EQPS +  TRI+SL++ A+TI ELV E+I + + LEAFSIQL I
Sbjct: 541 SAPSTSHGSLDLGDAFEQPSTHSLTRIRSLRKYAATIAELVYERIESDKHLEAFSIQLAI 600

Query: 601 LAIWKKALDICHTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITS 660
           LAIWK+AL ICHTQA S LEGSP Q   + R S  K    S  K + L  S     +I+S
Sbjct: 601 LAIWKQALHICHTQAISGLEGSPSQDINKLRSSSLKHDTHSSNKVTDL--SHDGSEEISS 660

Query: 661 QVEREFLREVEYAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAAL 720
           Q++R+F++E+E AEELAK +EPGNT+MPDA+ET+F++AL  G+ GGV+E+MG+ E+A   
Sbjct: 661 QIQRQFIQEIELAEELAKSIEPGNTKMPDAMETIFEAALDLGKLGGVKEVMGDTENAGNQ 714

Query: 721 YSKSAQLLVFLLVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQR 759
           YSK+ +LLVFLLVEAP LILNPP SLTNS RYRLR+YID LS R  +L+S R
Sbjct: 721 YSKAVRLLVFLLVEAPMLILNPPLSLTNSVRYRLRTYIDFLSRRLKHLQSHR 714

BLAST of Spg033260 vs. ExPASy Swiss-Prot
Match: F4JBP3 (Serine/threonine-protein kinase ATG1b OS=Arabidopsis thaliana OX=3702 GN=ATG1B PE=2 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 2.0e-210
Identity = 432/767 (56.32%), Postives = 527/767 (68.71%), Query Frame = 0

Query: 4   STGRT-RVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEI 63
           + GR+ RV+ DY VGRQIGSGSFSVVW  RH VHGT VAIKEI+M+RL+KKLQ+SLMSEI
Sbjct: 9   AAGRSGRVIGDYAVGRQIGSGSFSVVWEGRHLVHGTVVAIKEIAMARLNKKLQESLMSEI 68

Query: 64  FILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVK 123
            IL+KINHPNIIR  D+IE                                         
Sbjct: 69  IILRKINHPNIIRFIDMIE----------------------------------------- 128

Query: 124 TVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNLIH 183
                  PGKI+LVLEYC+GGDLS YI  +HG +PEA AKHF+ QLAAGL++LRDNN+IH
Sbjct: 129 ------APGKINLVLEYCKGGDLSMYI-HKHGSVPEATAKHFMLQLAAGLQVLRDNNIIH 188

Query: 184 RDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 243
           RDLKPQNLLLST D+++ LKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA
Sbjct: 189 RDLKPQNLLLSTDDNDAALKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 248

Query: 244 DLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLLRR 303
           DLWSVGAILFQLVTGRTPFTGN+QIQLLQNI++STELHFP+D  DLS DCKDLC+KLLRR
Sbjct: 249 DLWSVGAILFQLVTGRTPFTGNSQIQLLQNIIRSTELHFPADCRDLSTDCKDLCQKLLRR 308

Query: 304 NPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPF 363
           NPVERLTFEEFF+HPFL   Q  + +RS+  SR ++ F  S S P+ N+EE SQ+D LPF
Sbjct: 309 NPVERLTFEEFFHHPFLSDKQSYDFTRSRLDSRTMNDFHSSGSSPSRNIEEISQEDGLPF 368

Query: 364 SLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKK----VDRRTRDTTSRYSSVPDKMESAT 423
            LDDDSSGPEGSPS  +  S MKS+YGFS +++       +  D +SRYS V  + E+  
Sbjct: 369 FLDDDSSGPEGSPSSFKHTSPMKSSYGFSVERREAALSPLKNMDLSSRYSRVSHRAETNN 428

Query: 424 LVSDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPS 483
              + H       L DRS        + +S +T+ R  DS + +DQDYVL+SGPP+D PS
Sbjct: 429 FKFEGH------RLSDRSQFKPSSLPDSRSFSTQGRG-DSPDSMDQDYVLISGPPVDIPS 488

Query: 484 SSLASASKPI--PCKTRSSPQTSVSLGAAS-SAPVPIIGRATSSSSRMGSLGSQSSAP-- 543
           SS + + KP   P K+ S P   +     + +AP+PI     ++ SR GSL SQ+  P  
Sbjct: 489 SS-SGSPKPFNYPFKSHSPPVEFIKRNVTNLTAPMPIASATGNNLSRFGSLESQNCIPGT 548

Query: 544 --GSMDIEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWK 603
             GS+D+ DA EQPS N  TRI+SLQ+ A+ I ELV E+   G+ LEAFSIQLVILAIW 
Sbjct: 549 SHGSLDLVDAFEQPSTNSLTRIRSLQKCAAAIAELVHERGENGKHLEAFSIQLVILAIWN 608

Query: 604 KALDICHTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVERE 663
           +AL ICHTQA S +EGS  Q      R++      S G   +L+           Q+++E
Sbjct: 609 QALHICHTQAVSGIEGSLLQDINRVGRNI------SHGGSEKLL----------PQIQKE 668

Query: 664 FLREVEYAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSA 723
           F++EVE AEELAK VE  N +MPDA+E + Q+ALA G  GGV+E+MG+ E+A  LYSK+ 
Sbjct: 669 FVQEVERAEELAKFVESDNAKMPDAMEMILQAALALGISGGVDEVMGDAENAGNLYSKAV 703

Query: 724 QLLVFLLVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQR 759
           +LLVFL VEA +LILNPP +LTNS RYRLR+YID L  R  +L+S R
Sbjct: 729 RLLVFLAVEAQTLILNPPLTLTNSVRYRLRTYIDSLITRLKHLQSHR 703

BLAST of Spg033260 vs. ExPASy Swiss-Prot
Match: Q94C95 (Serine/threonine-protein kinase ATG1a OS=Arabidopsis thaliana OX=3702 GN=ATG1A PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.2e-116
Identity = 291/756 (38.49%), Postives = 396/756 (52.38%), Query Frame = 0

Query: 9   RVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEIFILKKI 68
           R+V DY +G +IGSGSF+VVW A+HR  G EVA+KEI    LS K++D+L+ EI IL  I
Sbjct: 5   RLVGDYALGPRIGSGSFAVVWLAKHRSSGLEVAVKEIDKKLLSPKVRDNLLKEISILSTI 64

Query: 69  NHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVKTVTSIQ 128
           +HPNIIR ++ IE                       G+R                     
Sbjct: 65  DHPNIIRFYEAIET----------------------GDR--------------------- 124

Query: 129 VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNLIHRDLKPQ 188
               I LVLEYC GGDL+ YI  RHG++PEA AKHF++QLA GL++L++ + IHRDLKPQ
Sbjct: 125 ----IFLVLEYCSGGDLAGYI-NRHGKVPEAVAKHFMRQLALGLQVLQEKHFIHRDLKPQ 184

Query: 189 NLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVG 248
           NLLLS+ +   +LKI DFGFARSL P  +AET CGSPLYMAPEI++ QKYDAKADLWS G
Sbjct: 185 NLLLSSKEVTPLLKIGDFGFARSLTPESMAETFCGSPLYMAPEIIRNQKYDAKADLWSAG 244

Query: 249 AILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDP-NDLSFDCKDLCKKLLRRNPVER 308
           AILFQLVTG+ PF GNN IQL  NIV+ TEL FP D  N++  DC DLC+ LLRRNP+ER
Sbjct: 245 AILFQLVTGKPPFDGNNHIQLFHNIVRDTELKFPEDTRNEIHPDCVDLCRSLLRRNPIER 304

Query: 309 LTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPFSLDDD 368
           LTF EFFNH FL         R  R    V+   FS     S +                
Sbjct: 305 LTFREFFNHMFL---------REPRQIPDVEHSGFSTCTGKSLL---------------- 364

Query: 369 SSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPD---KMESATLVSDTH 428
              P   PS                          +T+R+ S  +   K  S++  S++ 
Sbjct: 365 ---PSAQPS-------------------------TSTNRFKSSAENVHKHGSSSSASNSQ 424

Query: 429 VRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSSLASA 488
           +      +P  S     +    Q  + +S  VDSLELI+++YVLV     + PS+SL  +
Sbjct: 425 IS-----MPHTSFEKTRKDTEGQCSSNQSGVVDSLELIEREYVLV-----NRPSASLEGS 484

Query: 489 SKPIPCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMDIEDALEQ 548
           S       + S   ++         +P   + +SSS       S  S P    +   L +
Sbjct: 485 SDCFDTSLQDSGFPNI---------LPRNEKVSSSSLEAQKPLSDVSGPRPASVSYLLTE 544

Query: 549 PS----ANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQ 608
                  +  T+++ L Q A  +TEL  E    G+  E+F++ LV+LA+W+KAL+IC + 
Sbjct: 545 VQRLTIVHPPTKLQLLHQYAQALTELASEMGNTGQVKESFAVTLVVLAVWRKALEICDSW 604

Query: 609 AASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYAE 668
             S  E         +  +               I   + P    + V +EF+  +  AE
Sbjct: 605 MMSVGENEVNPDPTTAPET--------------SIPDLNSPAPAKTWVTQEFVTALNQAE 626

Query: 669 ELAKVVEPGN--TEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLL 728
            L+  +   +  T MPDA+ET+++ ALA+G+ GG EE +   ESAA LY K+  LL F++
Sbjct: 665 NLSTQLNETSAATHMPDAMETIYERALAYGKSGGAEEYLSNKESAATLYKKAILLLSFII 626

Query: 729 VEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYL 755
            EA +L LNP FSLT  D+ R+  YI  L +R+ +L
Sbjct: 725 EEAVTLSLNPSFSLTPDDKKRILYYISNLQHRRSHL 626

BLAST of Spg033260 vs. ExPASy Swiss-Prot
Match: A7KAL2 (Serine/threonine-protein kinase atg1 OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=atg1 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.5e-59
Identity = 151/374 (40.37%), Postives = 199/374 (53.21%), Query Frame = 0

Query: 19  QIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEIFILKKINHPNIIRLHD 78
           +IG GSF+ V+   H    T VAIK +++S+L+KKL+++L SEI ILK ++HP+I+ L D
Sbjct: 27  EIGRGSFATVYQGVHTKSKTYVAIKSVNLSKLNKKLKENLSSEIDILKGLHHPHIVALID 86

Query: 79  IIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVKTVTSIQVPGKIHLVLE 138
                +E+ S                                            IHLV+E
Sbjct: 87  C----HESTSH-------------------------------------------IHLVME 146

Query: 139 YCRGGDLSFYIQQRH--------------------GRIPEANAKHFLQQLAAGLKILRDN 198
           YC  GDLS +I++R                     G + E   +HFL+QL++ LK LRD 
Sbjct: 147 YCALGDLSLFIKRRDTLGSHKYTRDMIAKYPNPPGGSLNEVVTRHFLKQLSSALKFLRDR 206

Query: 199 NLIHRDLKPQNLLL-----------------STSDDN----------SVLKIADFGFARS 258
           NLIHRD+KPQNLLL                   SDD+           +LKIADFGFARS
Sbjct: 207 NLIHRDIKPQNLLLCPSPSSYRSGHAQVMPYKGSDDSYEPTTGLESLPMLKIADFGFARS 266

Query: 259 LQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTGRTPFTGNNQIQLLQ 318
           L    LAETLCGSPLYMAPEI++ +KYDAKADLWSVG +L+++V GR PF   N ++LL+
Sbjct: 267 LPATSLAETLCGSPLYMAPEILRYEKYDAKADLWSVGTVLYEMVVGRPPFRATNHVELLR 326

Query: 319 NIVKSTE-LHFPSDPNDLSFDCKDLCKKLLRRNPVERLTFEEFFNH-------PFLCANQ 338
            I K  + + FP D N  S D K L + LL+RNPVERL F EFF++       P L A+ 
Sbjct: 327 KIEKGEDRIRFPED-NPASDDIKKLIRGLLKRNPVERLNFPEFFSNNVINDPIPGLLADD 352

BLAST of Spg033260 vs. ExPASy Swiss-Prot
Match: Q52EB3 (Serine/threonine-protein kinase ATG1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=ATG1 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.9e-59
Identity = 155/455 (34.07%), Postives = 227/455 (49.89%), Query Frame = 0

Query: 11  VADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEIFILKKINH 70
           V  +++G +IG GSF+ V+  +H+V G  VAIK + ++RL+KKL+++L  EI ILK + H
Sbjct: 16  VGQFVIGAEIGKGSFAQVYMGKHKVSGAAVAIKSVELARLNKKLKENLYGEINILKTLRH 75

Query: 71  PNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVKTVTSIQVP 130
           P+I+ LHD +E                                                 
Sbjct: 76  PHIVALHDCVESATH--------------------------------------------- 135

Query: 131 GKIHLVLEYCRGGDLSFYIQQR--------------------HGRIPEANAKHFLQQLAA 190
             I+L++EYC  GDLS +I++R                    +  + E   +HFL+QL++
Sbjct: 136 --INLMMEYCELGDLSLFIKKREKLSTNPATHDMARKYPNVPNSGLNEVVIRHFLKQLSS 195

Query: 191 GLKILRDNNLIHRDLKPQNLL-----------------LSTSDDNSV----------LKI 250
            LK LR++NL+HRD+KPQNLL                 L+ S D+ V          LK+
Sbjct: 196 ALKFLRESNLVHRDVKPQNLLLLPSPEFREVNKLARPILTASQDSLVPVAGLASLPMLKL 255

Query: 251 ADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTGRTPFTG 310
           ADFGFAR L    LAETLCGSPLYMAPEI++ ++YDAKADLWSVG +LF+++ GR PF  
Sbjct: 256 ADFGFARVLPSTSLAETLCGSPLYMAPEILRYERYDAKADLWSVGTVLFEMIVGRPPFRA 315

Query: 311 NNQIQLLQNIVKSTE-LHFPSDPNDLSFDCKDLCKKLLRRNPVERLTFEEFFNHPFLCAN 370
           +N ++LL+ I  + + + FP +   +S + K L + LL+RNPVER++FE FF HP + ++
Sbjct: 316 SNHVELLRKIEAAEDVIKFPRE-TTISSEMKGLTRALLKRNPVERISFENFFAHPVIISS 375

Query: 371 QPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPFSLDDDSSGPEGSPSFVRRRS 418
            P           + D  P  E   AS    +S+D       DD  + P       R+ S
Sbjct: 376 IP---------GLVEDDIPKPE---ASEQRSSSKDTRAASKSDDPIASP-------RKYS 403

BLAST of Spg033260 vs. ExPASy TrEMBL
Match: A0A6J1F345 (serine/threonine-protein kinase ATG1c-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441938 PE=4 SV=1)

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 658/774 (85.01%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTG TRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGGTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILKKINHPNIIRLHDI+E                                       
Sbjct: 61  EIFILKKINHPNIIRLHDIVE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AK FLQQLAAGLK+LRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAIAKRFLQQLAAGLKVLRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS DNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSGDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQ VTGRTPFTGNNQIQLLQNIVKST+LHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQFVTGRTPFTGNNQIQLLQNIVKSTKLHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFLCANQPDEL  SKRSSRLVDGFPFSESD A +MEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFNHPFLCANQPDELLGSKRSSRLVDGFPFSESDHAGSMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDR TRD+TSRYSS+PDK E    +
Sbjct: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRGTRDSTSRYSSLPDKREITNPL 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDD-PSS 480
            DT V+ GRN+L DRSL+  LRSANPQS+NTR R  DSLELIDQDYVLVSGP MDD  SS
Sbjct: 421 LDTRVQ-GRNILSDRSLNAGLRSANPQSVNTRPRVADSLELIDQDYVLVSGPSMDDYASS 480

Query: 481 SLASASKPI--PCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMD 540
           SLASASKPI   CK++S  Q SVSLGAA +AP+PII +A SSSSRMGSLGSQSSAPGS+D
Sbjct: 481 SLASASKPILSSCKSQSPTQPSVSLGAALTAPMPIIRKAASSSSRMGSLGSQSSAPGSLD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           +ED LEQPSANCT RIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 MEDTLEQPSANCTARIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAESRRSL KKQGSSLGKGS L+V S QPVDI++QVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAESRRSLRKKQGSSLGKGSPLMVDSLQPVDISTQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAA LYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAGLYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSY+DILSNRQGYLRSQRLALLKCENQQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYVDILSNRQGYLRSQRLALLKCENQQSPP 726

BLAST of Spg033260 vs. ExPASy TrEMBL
Match: A0A6J1F4S0 (serine/threonine-protein kinase ATG1c-like OS=Cucurbita moschata OX=3662 GN=LOC111442158 PE=4 SV=1)

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 648/764 (84.82%), Postives = 679/764 (88.87%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISM+RLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMTRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL+DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLYDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYC+GGDLSFYIQQRHGRIPEA AKHFLQQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCKGGDLSFYIQQRHGRIPEAIAKHFLQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLS SDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSASDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPF+CANQPDE  RSKRSSRL+D FPFSESDPASNMEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFKHPFVCANQPDESLRSKRSSRLLDEFPFSESDPASNMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFV RRSSMKSTYGFSPDKKVDR TRDTTSRY S+PDKMES TL+
Sbjct: 361 PFSLDDDSSGPEGSPSFV-RRSSMKSTYGFSPDKKVDRGTRDTTSRYGSIPDKMESTTLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
            DT V+L R+V  DRSL+GA RSAN  S+N R R VDS +LIDQDYV+VSGPPMDD SS+
Sbjct: 421 LDTRVQLERDVHSDRSLNGASRSANTLSVNNRPRVVDSPDLIDQDYVIVSGPPMDDTSST 480

Query: 481 LASASKPIPC-KTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMDIE 540
           LASASKPIP  K++S PQ+S+ LG A +AP+PIIGRAT+SS RMGSLGSQSSAPGSMDIE
Sbjct: 481 LASASKPIPSRKSQSPPQSSIRLGTAVTAPMPIIGRATTSSYRMGSLGSQSSAPGSMDIE 540

Query: 541 DALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT 600
           D LEQPSANC  R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT
Sbjct: 541 DTLEQPSANCRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHT 600

Query: 601 QAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYA 660
           QAASALEGSPGQGSAE RRSL KKQGSSLGK S LIV+SHQPVDI+SQVEREFLREVEYA
Sbjct: 601 QAASALEGSPGQGSAEFRRSLSKKQGSSLGKESHLIVNSHQPVDISSQVEREFLREVEYA 660

Query: 661 EELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLLV 720
           EELAK+VEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEME+AAALYSK+AQLLVFLLV
Sbjct: 661 EELAKIVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMETAAALYSKAAQLLVFLLV 716

Query: 721 EAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLK 764
           EAPSLILNPPFSLTNSDRYRLRSYIDIL+NRQGYLRSQRLALLK
Sbjct: 721 EAPSLILNPPFSLTNSDRYRLRSYIDILNNRQGYLRSQRLALLK 716

BLAST of Spg033260 vs. ExPASy TrEMBL
Match: A0A5D3DNX4 (Serine/threonine-protein kinase ATG1c OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001380 PE=4 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 653/774 (84.37%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLFDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AKHFLQQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAVAKHFLQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS++NSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSEENSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLS DCKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSNDCKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPFLCANQ DE  RSKRSSRL+D FPFSESDPAS MEENSQD+YL
Sbjct: 301 RRNPVERLTFEEFFKHPFLCANQADESLRSKRSSRLLDEFPFSESDPASKMEENSQDEYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPS +RR SSMKSTYGFSPDKKVDR TR TTSR+SSVPDKMESATL+
Sbjct: 361 PFSLDDDSSGPEGSPS-LRRMSSMKSTYGFSPDKKVDRGTRGTTSRHSSVPDKMESATLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
           SDT V+L R+V  DRSL+  LRS NP+S+N R R VDS+E IDQDYV+VSGPPMDDPSS+
Sbjct: 421 SDTRVQLERDVHSDRSLNSVLRSVNPRSVNNRPRVVDSVESIDQDYVIVSGPPMDDPSST 480

Query: 481 LASASKPIPC-KTRSSPQTSVSLGAASSAPVPIIGRA--TSSSSRMGSLGSQSSAPGSMD 540
           L SAS+PIP  K++S PQTS+SLG A +AP+PIIGRA  T+SS RMGSLGSQSSAPGSMD
Sbjct: 481 LPSASRPIPSHKSQSPPQTSISLGTALTAPMPIIGRATTTTSSYRMGSLGSQSSAPGSMD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           IED LEQPSANC  R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 IEDTLEQPSANCRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAE RRSL KKQGS LGK S+LIVS HQPVDI+SQVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAEFRRSLSKKQGSPLGKESRLIVSPHQPVDISSQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPG TEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGKTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSYI+IL NRQGYLRSQRLALLKCE+QQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIEILYNRQGYLRSQRLALLKCESQQSPP 726

BLAST of Spg033260 vs. ExPASy TrEMBL
Match: A0A1S3B6H7 (serine/threonine-protein kinase ATG1c OS=Cucumis melo OX=3656 GN=LOC103486358 PE=4 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 653/774 (84.37%), Postives = 683/774 (88.24%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRL DIIE                                       
Sbjct: 61  EIFILKRINHPNIIRLFDIIE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AKHFLQQLAAGLKILRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAVAKHFLQQLAAGLKILRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS++NSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSEENSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLS DCKDLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSNDCKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFF HPFLCANQ DE  RSKRSSRL+D FPFSESDPAS MEENSQD+YL
Sbjct: 301 RRNPVERLTFEEFFKHPFLCANQADESLRSKRSSRLLDEFPFSESDPASKMEENSQDEYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPS +RR SSMKSTYGFSPDKKVDR TR TTSR+SSVPDKMESATL+
Sbjct: 361 PFSLDDDSSGPEGSPS-LRRMSSMKSTYGFSPDKKVDRGTRGTTSRHSSVPDKMESATLI 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSS 480
           SDT V+L R+V  DRSL+  LRS NP+S+N R R VDS+E IDQDYV+VSGPPMDDPSS+
Sbjct: 421 SDTRVQLERDVHSDRSLNSVLRSVNPRSVNNRPRVVDSVESIDQDYVIVSGPPMDDPSST 480

Query: 481 LASASKPIPC-KTRSSPQTSVSLGAASSAPVPIIGRA--TSSSSRMGSLGSQSSAPGSMD 540
           L SAS+PIP  K++S PQTS+SLG A +AP+PIIGRA  T+SS RMGSLGSQSSAPGSMD
Sbjct: 481 LPSASRPIPSHKSQSPPQTSISLGTALTAPMPIIGRATTTTSSYRMGSLGSQSSAPGSMD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           IED LEQPSANC  R+KSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 IEDTLEQPSANCRARVKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAE RRSL KKQGS LGK S+LIVS HQPVDI+SQVEREFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAEFRRSLSKKQGSPLGKESRLIVSPHQPVDISSQVEREFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPG TEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGKTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSYI+IL NRQGYLRSQRLALLKCE+QQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIEILYNRQGYLRSQRLALLKCESQQSPP 726

BLAST of Spg033260 vs. ExPASy TrEMBL
Match: A0A6J1I687 (serine/threonine-protein kinase ATG1c-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470017 PE=4 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 653/774 (84.37%), Postives = 682/774 (88.11%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MA S G TRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS
Sbjct: 1   MAQSAGGTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFILK+INHPNIIRLHDI+E                                       
Sbjct: 61  EIFILKRINHPNIIRLHDIVE--------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                   VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEA AK FLQQLAAGLK+LRDNNL
Sbjct: 121 --------VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEAIAKRFLQQLAAGLKVLRDNNL 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLSTS DNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTSGDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQ VTGRTPFTGNNQIQLLQNIVKSTELHFPSD NDLSF+CKDLC+KLL
Sbjct: 241 KADLWSVGAILFQFVTGRTPFTGNNQIQLLQNIVKSTELHFPSDINDLSFECKDLCRKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFLCANQPDEL  SKRSSRLVDGFPFS+SD A +MEENSQDDYL
Sbjct: 301 RRNPVERLTFEEFFNHPFLCANQPDELLGSKRSSRLVDGFPFSKSDHAGSMEENSQDDYL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPDKMESATLV 420
           PFSLDDDSSGPEGSPSFVRRRSSMKST GFSPDKKVDR TRD+TSRYSS+PDK E  T +
Sbjct: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTCGFSPDKKVDRGTRDSTSRYSSLPDKREITTPL 420

Query: 421 SDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDD-PSS 480
            D  V+ GRN+L DRSL+  LRSANPQS+NTR R  DSLELIDQDYVLVSGP MDD  SS
Sbjct: 421 VDIRVQ-GRNILSDRSLNAGLRSANPQSVNTRPRVADSLELIDQDYVLVSGPSMDDYASS 480

Query: 481 SLASASKPI--PCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMD 540
           SLA+ASKPI   CK++S  QTSVSLGAA +AP+PIIG+ATSSSSRMGSLGSQSSAPGS+D
Sbjct: 481 SLANASKPILSSCKSQSPTQTSVSLGAALTAPMPIIGKATSSSSRMGSLGSQSSAPGSLD 540

Query: 541 IEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600
           +ED LEQPSANCT RIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC
Sbjct: 541 MEDTLEQPSANCTARIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDIC 600

Query: 601 HTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVE 660
           HTQAASALEGSPGQGSAESRRSL KKQGSSLGKG+ L+V S QPVDI++QVE+EFLREVE
Sbjct: 601 HTQAASALEGSPGQGSAESRRSLRKKQGSSLGKGTPLMVDSLQPVDISTQVEKEFLREVE 660

Query: 661 YAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFL 720
           YAEELAKVVEPGNTEMPDAIET+FQSALAFGRHGGVEELMGEMESAA LYSK+AQLLVFL
Sbjct: 661 YAEELAKVVEPGNTEMPDAIETMFQSALAFGRHGGVEELMGEMESAAGLYSKAAQLLVFL 720

Query: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKCENQQSPP 772
           LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLK ENQQSPP
Sbjct: 721 LVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQRLALLKSENQQSPP 726

BLAST of Spg033260 vs. TAIR 10
Match: AT2G37840.1 (Protein kinase superfamily protein )

HSP 1 Score: 794.7 bits (2051), Expect = 6.8e-230
Identity = 455/772 (58.94%), Postives = 555/772 (71.89%), Query Frame = 0

Query: 1   MAHSTGRTRVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMS 60
           MA  TG  RVV DYLVGRQIGSGSFSVVW ARHRV GTEVAIKEI+M RL+KKLQ+SLMS
Sbjct: 1   MAQFTG--RVVGDYLVGRQIGSGSFSVVWEARHRVDGTEVAIKEIAMDRLNKKLQESLMS 60

Query: 61  EIFILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAF 120
           EIFIL++INHPNIIRL D+                                         
Sbjct: 61  EIFILRRINHPNIIRLIDM----------------------------------------- 120

Query: 121 VKTVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNL 180
                 I+ PGK+HLVLEYC+GGDLS Y+ QRHG +PEA AKHF+QQLAAGL++LRDNN+
Sbjct: 121 ------IKSPGKVHLVLEYCKGGDLSVYV-QRHGIVPEATAKHFMQQLAAGLQVLRDNNI 180

Query: 181 IHRDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240
           IHRDLKPQNLLLST+++++ LKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA
Sbjct: 181 IHRDLKPQNLLLSTNENDADLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDA 240

Query: 241 KADLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLL 300
           KADLWSVGAILFQLVTGRTPFTGN+QIQLLQNI++STELHFP D  DLS DC DLC+KLL
Sbjct: 241 KADLWSVGAILFQLVTGRTPFTGNSQIQLLQNIIRSTELHFPGDCRDLSLDCIDLCQKLL 300

Query: 301 RRNPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYL 360
           RRNPVERLTFEEFFNHPFL   Q  + SRS+   R +DGF  S S P+ NMEE+SQ+D L
Sbjct: 301 RRNPVERLTFEEFFNHPFLSDRQSYDFSRSRLGLRTMDGFLSSGSSPSRNMEESSQEDCL 360

Query: 361 PFSLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRT--------RDTTSRYSSVPD 420
           PF LDDDSSGPEGSPS++++ SSMKS+ G   D +++R+          + TS YSS   
Sbjct: 361 PFLLDDDSSGPEGSPSYLKKTSSMKSSSGIKVDTRIERKEVESSPLKHTELTSGYSSFNQ 420

Query: 421 KMESATLVSDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGP 480
           K+E+     +T +   R     R+        + +S+    R  DS + +DQD+VLVSGP
Sbjct: 421 KVENDRFRFETQINSDR-----RNRREPTGLTDSRSLIAPGRVDDSQDSMDQDFVLVSGP 480

Query: 481 PMDDPSSSLASASKP--IPCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQS 540
           P+D PSSS +S+SKP   P K++S P    +   +S+AP+PIIG  ++S  + GSL SQ 
Sbjct: 481 PVDMPSSS-SSSSKPYNFPFKSQSPPVELFNRSISSTAPMPIIGATSNSIGQFGSLDSQY 540

Query: 541 SAP----GSMDIEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVI 600
           SAP    GS+D+ DA EQPS +  TRI+SL++ A+TI ELV E+I + + LEAFSIQL I
Sbjct: 541 SAPSTSHGSLDLGDAFEQPSTHSLTRIRSLRKYAATIAELVYERIESDKHLEAFSIQLAI 600

Query: 601 LAIWKKALDICHTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITS 660
           LAIWK+AL ICHTQA S LEGSP Q   + R S  K    S  K + L  S     +I+S
Sbjct: 601 LAIWKQALHICHTQAISGLEGSPSQDINKLRSSSLKHDTHSSNKVTDL--SHDGSEEISS 660

Query: 661 QVEREFLREVEYAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAAL 720
           Q++R+F++E+E AEELAK +EPGNT+MPDA+ET+F++AL  G+ GGV+E+MG+ E+A   
Sbjct: 661 QIQRQFIQEIELAEELAKSIEPGNTKMPDAMETIFEAALDLGKLGGVKEVMGDTENAGNQ 714

Query: 721 YSKSAQLLVFLLVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQR 759
           YSK+ +LLVFLLVEAP LILNPP SLTNS RYRLR+YID LS R  +L+S R
Sbjct: 721 YSKAVRLLVFLLVEAPMLILNPPLSLTNSVRYRLRTYIDFLSRRLKHLQSHR 714

BLAST of Spg033260 vs. TAIR 10
Match: AT3G53930.2 (Protein kinase superfamily protein )

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-212
Identity = 432/767 (56.32%), Postives = 527/767 (68.71%), Query Frame = 0

Query: 4   STGRT-RVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEI 63
           + GR+ RV+ DY VGRQIGSGSFSVVW  RH VHGT VAIKEI+M+RL+KKLQ+SLMSEI
Sbjct: 9   AAGRSGRVIGDYAVGRQIGSGSFSVVWEGRHLVHGTVVAIKEIAMARLNKKLQESLMSEI 68

Query: 64  FILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVK 123
            IL+KINHPNIIR  D+IE                                         
Sbjct: 69  IILRKINHPNIIRFIDMIE----------------------------------------- 128

Query: 124 TVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNLIH 183
                  PGKI+LVLEYC+GGDLS YI  +HG +PEA AKHF+ QLAAGL++LRDNN+IH
Sbjct: 129 ------APGKINLVLEYCKGGDLSMYI-HKHGSVPEATAKHFMLQLAAGLQVLRDNNIIH 188

Query: 184 RDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 243
           RDLKPQNLLLST D+++ LKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA
Sbjct: 189 RDLKPQNLLLSTDDNDAALKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 248

Query: 244 DLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLLRR 303
           DLWSVGAILFQLVTGRTPFTGN+QIQLLQNI++STELHFP+D  DLS DCKDLC+KLLRR
Sbjct: 249 DLWSVGAILFQLVTGRTPFTGNSQIQLLQNIIRSTELHFPADCRDLSTDCKDLCQKLLRR 308

Query: 304 NPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPF 363
           NPVERLTFEEFF+HPFL   Q  + +RS+  SR ++ F  S S P+ N+EE SQ+D LPF
Sbjct: 309 NPVERLTFEEFFHHPFLSDKQSYDFTRSRLDSRTMNDFHSSGSSPSRNIEEISQEDGLPF 368

Query: 364 SLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKK----VDRRTRDTTSRYSSVPDKMESAT 423
            LDDDSSGPEGSPS  +  S MKS+YGFS +++       +  D +SRYS V  + E+  
Sbjct: 369 FLDDDSSGPEGSPSSFKHTSPMKSSYGFSVERREAALSPLKNMDLSSRYSRVSHRAETNN 428

Query: 424 LVSDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPS 483
              + H       L DRS        + +S +T+ R  DS + +DQDYVL+SGPP+D PS
Sbjct: 429 FKFEGH------RLSDRSQFKPSSLPDSRSFSTQGRVGDSPDSMDQDYVLISGPPVDIPS 488

Query: 484 SSLASASKPI--PCKTRSSPQTSVSLGAAS-SAPVPIIGRATSSSSRMGSLGSQSSAP-- 543
           SS + + KP   P K+ S P   +     + +AP+PI     ++ SR GSL SQ+  P  
Sbjct: 489 SS-SGSPKPFNYPFKSHSPPVEFIKRNVTNLTAPMPIASATGNNLSRFGSLESQNCIPGT 548

Query: 544 --GSMDIEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWK 603
             GS+D+ DA EQPS N  TRI+SLQ+ A+ I ELV E+   G+ LEAFSIQLVILAIW 
Sbjct: 549 SHGSLDLVDAFEQPSTNSLTRIRSLQKCAAAIAELVHERGENGKHLEAFSIQLVILAIWN 608

Query: 604 KALDICHTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVERE 663
           +AL ICHTQA S +EGS  Q      R++      S G   +L+           Q+++E
Sbjct: 609 QALHICHTQAVSGIEGSLLQDINRVGRNI------SHGGSEKLL----------PQIQKE 668

Query: 664 FLREVEYAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSA 723
           F++EVE AEELAK VE  N +MPDA+E + Q+ALA G  GGV+E+MG+ E+A  LYSK+ 
Sbjct: 669 FVQEVERAEELAKFVESDNAKMPDAMEMILQAALALGISGGVDEVMGDAENAGNLYSKAV 704

Query: 724 QLLVFLLVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQR 759
           +LLVFL VEA +LILNPP +LTNS RYRLR+YID L  R  +L+S R
Sbjct: 729 RLLVFLAVEAQTLILNPPLTLTNSVRYRLRTYIDSLITRLKHLQSHR 704

BLAST of Spg033260 vs. TAIR 10
Match: AT3G53930.1 (Protein kinase superfamily protein )

HSP 1 Score: 733.8 bits (1893), Expect = 1.4e-211
Identity = 432/767 (56.32%), Postives = 527/767 (68.71%), Query Frame = 0

Query: 4   STGRT-RVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEI 63
           + GR+ RV+ DY VGRQIGSGSFSVVW  RH VHGT VAIKEI+M+RL+KKLQ+SLMSEI
Sbjct: 9   AAGRSGRVIGDYAVGRQIGSGSFSVVWEGRHLVHGTVVAIKEIAMARLNKKLQESLMSEI 68

Query: 64  FILKKINHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVK 123
            IL+KINHPNIIR  D+IE                                         
Sbjct: 69  IILRKINHPNIIRFIDMIE----------------------------------------- 128

Query: 124 TVTSIQVPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNLIH 183
                  PGKI+LVLEYC+GGDLS YI  +HG +PEA AKHF+ QLAAGL++LRDNN+IH
Sbjct: 129 ------APGKINLVLEYCKGGDLSMYI-HKHGSVPEATAKHFMLQLAAGLQVLRDNNIIH 188

Query: 184 RDLKPQNLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 243
           RDLKPQNLLLST D+++ LKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA
Sbjct: 189 RDLKPQNLLLSTDDNDAALKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKA 248

Query: 244 DLWSVGAILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDPNDLSFDCKDLCKKLLRR 303
           DLWSVGAILFQLVTGRTPFTGN+QIQLLQNI++STELHFP+D  DLS DCKDLC+KLLRR
Sbjct: 249 DLWSVGAILFQLVTGRTPFTGNSQIQLLQNIIRSTELHFPADCRDLSTDCKDLCQKLLRR 308

Query: 304 NPVERLTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPF 363
           NPVERLTFEEFF+HPFL   Q  + +RS+  SR ++ F  S S P+ N+EE SQ+D LPF
Sbjct: 309 NPVERLTFEEFFHHPFLSDKQSYDFTRSRLDSRTMNDFHSSGSSPSRNIEEISQEDGLPF 368

Query: 364 SLDDDSSGPEGSPSFVRRRSSMKSTYGFSPDKK----VDRRTRDTTSRYSSVPDKMESAT 423
            LDDDSSGPEGSPS  +  S MKS+YGFS +++       +  D +SRYS V  + E+  
Sbjct: 369 FLDDDSSGPEGSPSSFKHTSPMKSSYGFSVERREAALSPLKNMDLSSRYSRVSHRAETNN 428

Query: 424 LVSDTHVRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPS 483
              + H       L DRS        + +S +T+ R  DS + +DQDYVL+SGPP+D PS
Sbjct: 429 FKFEGH------RLSDRSQFKPSSLPDSRSFSTQGRG-DSPDSMDQDYVLISGPPVDIPS 488

Query: 484 SSLASASKPI--PCKTRSSPQTSVSLGAAS-SAPVPIIGRATSSSSRMGSLGSQSSAP-- 543
           SS + + KP   P K+ S P   +     + +AP+PI     ++ SR GSL SQ+  P  
Sbjct: 489 SS-SGSPKPFNYPFKSHSPPVEFIKRNVTNLTAPMPIASATGNNLSRFGSLESQNCIPGT 548

Query: 544 --GSMDIEDALEQPSANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWK 603
             GS+D+ DA EQPS N  TRI+SLQ+ A+ I ELV E+   G+ LEAFSIQLVILAIW 
Sbjct: 549 SHGSLDLVDAFEQPSTNSLTRIRSLQKCAAAIAELVHERGENGKHLEAFSIQLVILAIWN 608

Query: 604 KALDICHTQAASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVERE 663
           +AL ICHTQA S +EGS  Q      R++      S G   +L+           Q+++E
Sbjct: 609 QALHICHTQAVSGIEGSLLQDINRVGRNI------SHGGSEKLL----------PQIQKE 668

Query: 664 FLREVEYAEELAKVVEPGNTEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSA 723
           F++EVE AEELAK VE  N +MPDA+E + Q+ALA G  GGV+E+MG+ E+A  LYSK+ 
Sbjct: 669 FVQEVERAEELAKFVESDNAKMPDAMEMILQAALALGISGGVDEVMGDAENAGNLYSKAV 703

Query: 724 QLLVFLLVEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYLRSQR 759
           +LLVFL VEA +LILNPP +LTNS RYRLR+YID L  R  +L+S R
Sbjct: 729 RLLVFLAVEAQTLILNPPLTLTNSVRYRLRTYIDSLITRLKHLQSHR 703

BLAST of Spg033260 vs. TAIR 10
Match: AT2G37840.2 (Protein kinase superfamily protein )

HSP 1 Score: 569.7 bits (1467), Expect = 3.6e-162
Identity = 328/564 (58.16%), Postives = 409/564 (72.52%), Query Frame = 0

Query: 209 ARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTGRTPFTGNNQIQ 268
           +RSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTGRTPFTGN+QIQ
Sbjct: 22  SRSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVGAILFQLVTGRTPFTGNSQIQ 81

Query: 269 LLQNIVKSTELHFPSDPNDLSFDCKDLCKKLLRRNPVERLTFEEFFNHPFLCANQPDELS 328
           LLQNI++STELHFP D  DLS DC DLC+KLLRRNPVERLTFEEFFNHPFL   Q  + S
Sbjct: 82  LLQNIIRSTELHFPGDCRDLSLDCIDLCQKLLRRNPVERLTFEEFFNHPFLSDRQSYDFS 141

Query: 329 RSKRSSRLVDGFPFSESDPASNMEENSQDDYLPFSLDDDSSGPEGSPSFVRRRSSMKSTY 388
           RS+   R +DGF  S S P+ NMEE+SQ+D LPF LDDDSSGPEGSPS++++ SSMKS+ 
Sbjct: 142 RSRLGLRTMDGFLSSGSSPSRNMEESSQEDCLPFLLDDDSSGPEGSPSYLKKTSSMKSSS 201

Query: 389 GFSPDKKVDRRT--------RDTTSRYSSVPDKMESATLVSDTHVRLGRNVLPDRSLSGA 448
           G   D +++R+          + TS YSS   K+E+     +T +   R     R+    
Sbjct: 202 GIKVDTRIERKEVESSPLKHTELTSGYSSFNQKVENDRFRFETQINSDR-----RNRREP 261

Query: 449 LRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSSLASASKP--IPCKTRSSPQT 508
               + +S+    R  DS + +DQD+VLVSGPP+D PSSS +S+SKP   P K++S P  
Sbjct: 262 TGLTDSRSLIAPGRVDDSQDSMDQDFVLVSGPPVDMPSSS-SSSSKPYNFPFKSQSPPVE 321

Query: 509 SVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAP----GSMDIEDALEQPSANCTTRIK 568
             +   +S+AP+PIIG  ++S  + GSL SQ SAP    GS+D+ DA EQPS +  TRI+
Sbjct: 322 LFNRSISSTAPMPIIGATSNSIGQFGSLDSQYSAPSTSHGSLDLGDAFEQPSTHSLTRIR 381

Query: 569 SLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQAASALEGSPGQGSA 628
           SL++ A+TI ELV E+I + + LEAFSIQL ILAIWK+AL ICHTQA S LEGSP Q   
Sbjct: 382 SLRKYAATIAELVYERIESDKHLEAFSIQLAILAIWKQALHICHTQAISGLEGSPSQDIN 441

Query: 629 ESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYAEELAKVVEPGNTEMP 688
           + R S  K    S  K + L  S     +I+SQ++R+F++E+E AEELAK +EPGNT+MP
Sbjct: 442 KLRSSSLKHDTHSSNKVTDL--SHDGSEEISSQIQRQFIQEIELAEELAKSIEPGNTKMP 501

Query: 689 DAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLLVEAPSLILNPPFSLTN 748
           DA+ET+F++AL  G+ GGV+E+MG+ E+A   YSK+ +LLVFLLVEAP LILNPP SLTN
Sbjct: 502 DAMETIFEAALDLGKLGGVKEVMGDTENAGNQYSKAVRLLVFLLVEAPMLILNPPLSLTN 561

Query: 749 SDRYRLRSYIDILSNRQGYLRSQR 759
           S RYRLR+YID LS R  +L+S R
Sbjct: 562 SVRYRLRTYIDFLSRRLKHLQSHR 577

BLAST of Spg033260 vs. TAIR 10
Match: AT3G61960.1 (Protein kinase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 1.6e-117
Identity = 291/756 (38.49%), Postives = 396/756 (52.38%), Query Frame = 0

Query: 9   RVVADYLVGRQIGSGSFSVVWHARHRVHGTEVAIKEISMSRLSKKLQDSLMSEIFILKKI 68
           R+V DY +G +IGSGSF+VVW A+HR  G EVA+KEI    LS K++D+L+ EI IL  I
Sbjct: 5   RLVGDYALGPRIGSGSFAVVWLAKHRSSGLEVAVKEIDKKLLSPKVRDNLLKEISILSTI 64

Query: 69  NHPNIIRLHDIIEVEYEAISRLSRLVLVKSPSQSSLGERESLREIFSVLSAFVKTVTSIQ 128
           +HPNIIR ++ IE                       G+R                     
Sbjct: 65  DHPNIIRFYEAIET----------------------GDR--------------------- 124

Query: 129 VPGKIHLVLEYCRGGDLSFYIQQRHGRIPEANAKHFLQQLAAGLKILRDNNLIHRDLKPQ 188
               I LVLEYC GGDL+ YI  RHG++PEA AKHF++QLA GL++L++ + IHRDLKPQ
Sbjct: 125 ----IFLVLEYCSGGDLAGYI-NRHGKVPEAVAKHFMRQLALGLQVLQEKHFIHRDLKPQ 184

Query: 189 NLLLSTSDDNSVLKIADFGFARSLQPRGLAETLCGSPLYMAPEIMQLQKYDAKADLWSVG 248
           NLLLS+ +   +LKI DFGFARSL P  +AET CGSPLYMAPEI++ QKYDAKADLWS G
Sbjct: 185 NLLLSSKEVTPLLKIGDFGFARSLTPESMAETFCGSPLYMAPEIIRNQKYDAKADLWSAG 244

Query: 249 AILFQLVTGRTPFTGNNQIQLLQNIVKSTELHFPSDP-NDLSFDCKDLCKKLLRRNPVER 308
           AILFQLVTG+ PF GNN IQL  NIV+ TEL FP D  N++  DC DLC+ LLRRNP+ER
Sbjct: 245 AILFQLVTGKPPFDGNNHIQLFHNIVRDTELKFPEDTRNEIHPDCVDLCRSLLRRNPIER 304

Query: 309 LTFEEFFNHPFLCANQPDELSRSKRSSRLVDGFPFSESDPASNMEENSQDDYLPFSLDDD 368
           LTF EFFNH FL         R  R    V+   FS     S +                
Sbjct: 305 LTFREFFNHMFL---------REPRQIPDVEHSGFSTCTGKSLL---------------- 364

Query: 369 SSGPEGSPSFVRRRSSMKSTYGFSPDKKVDRRTRDTTSRYSSVPD---KMESATLVSDTH 428
              P   PS                          +T+R+ S  +   K  S++  S++ 
Sbjct: 365 ---PSAQPS-------------------------TSTNRFKSSAENVHKHGSSSSASNSQ 424

Query: 429 VRLGRNVLPDRSLSGALRSANPQSMNTRSRDVDSLELIDQDYVLVSGPPMDDPSSSLASA 488
           +      +P  S     +    Q  + +S  VDSLELI+++YVLV     + PS+SL  +
Sbjct: 425 IS-----MPHTSFEKTRKDTEGQCSSNQSGVVDSLELIEREYVLV-----NRPSASLEGS 484

Query: 489 SKPIPCKTRSSPQTSVSLGAASSAPVPIIGRATSSSSRMGSLGSQSSAPGSMDIEDALEQ 548
           S       + S   ++         +P   + +SSS       S  S P    +   L +
Sbjct: 485 SDCFDTSLQDSGFPNI---------LPRNEKVSSSSLEAQKPLSDVSGPRPASVSYLLTE 544

Query: 549 PS----ANCTTRIKSLQQSASTITELVKEKITAGRQLEAFSIQLVILAIWKKALDICHTQ 608
                  +  T+++ L Q A  +TEL  E    G+  E+F++ LV+LA+W+KAL+IC + 
Sbjct: 545 VQRLTIVHPPTKLQLLHQYAQALTELASEMGNTGQVKESFAVTLVVLAVWRKALEICDSW 604

Query: 609 AASALEGSPGQGSAESRRSLGKKQGSSLGKGSQLIVSSHQPVDITSQVEREFLREVEYAE 668
             S  E         +  +               I   + P    + V +EF+  +  AE
Sbjct: 605 MMSVGENEVNPDPTTAPET--------------SIPDLNSPAPAKTWVTQEFVTALNQAE 626

Query: 669 ELAKVVEPGN--TEMPDAIETVFQSALAFGRHGGVEELMGEMESAAALYSKSAQLLVFLL 728
            L+  +   +  T MPDA+ET+++ ALA+G+ GG EE +   ESAA LY K+  LL F++
Sbjct: 665 NLSTQLNETSAATHMPDAMETIYERALAYGKSGGAEEYLSNKESAATLYKKAILLLSFII 626

Query: 729 VEAPSLILNPPFSLTNSDRYRLRSYIDILSNRQGYL 755
            EA +L LNP FSLT  D+ R+  YI  L +R+ +L
Sbjct: 725 EEAVTLSLNPSFSLTPDDKKRILYYISNLQHRRSHL 626