Spg032894 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg032894
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	transcription elongation factor B polypeptide 3 isoform X2
Location	scaffold11: 14036860 .. 14039015 (+)
RNA-Seq Expression	Spg032894
Synteny	Spg032894

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGAGAGTGGCCAGTTCGCCGACTCAATAAGCCTACCATTTCAGGGGCAAGACCGAATGGGAGCTGGGAACATAACAGTACAAGATGGAATTCACTCCTTCCCCTATTGGGGTAAGTAGATGTGTGTGCCCTTAAGGGCTGATTCTAGGGCTTGAACAAAGGCGCGCAGTCCTCTCCTAGCCTGAGAGGAACTCCTGTTTAGTGGTTGGACCCTAAACAACTTGTTCATTAGAGGAATCAGTGGTACTTAAGGAACCAGAGGTAACCCAGGGGTAAAACGGTAATTTGACCCAGCTGGTGTTACGAACACACGTGAAGGATTGACTTACTGTTTGGTCGATATCCGTGGACACAGAAATATTCTATAGTGAGAAGAGTGCAGCTGTGGGTCTTTAGTGGAGTGACCCACAGTTAACGAATGTTGACTAAACTTGGTCAATGAGTTTGGCCAATTAGTCTCGTATCGTTGGAGCTTCTGATCTGTAGGTCCATTAGGTCCCACTGGTAGCTCGTAAAGAGACTTGAGGCAATTAAGTATTAATTGTAAATTGAAAGTGTTCAATTTATTTGGGGAATCGTATAATGTATATTGATGCATTATAATATAAAGTTTATGAAACTTTATAATATAATTTTTGAATTAATTTAAAAAAAAAATAATTCTTGAGAGAAAGTATTTGAAAGAGTTCAAATAAATTTAATATGAATTGGATTCATATTAATTTTAATTTTAATAAAATCCCTAAGATTAAATATATGATACTTAATTGGGTAAATTAATTAATTATGGAATTAATTAATTATTTATTAAAATTAGAGACCTTTTCCTATTAGGAAAGAAAATATTTGAAAGAGTTCAAATAAATTTAATATGAATTGGATTCATATTAATTTTAATTTTAATAAAATCCCTAAGATTAAATATATGATATTTAATTGGGAAATTAATTAATTATGGAATTAATTAATTATTTATTAAAATTAGAGACTTTTTCCTATTAGGAAAAGTTCTCCCTTTTCCTATTAGGAAAAAGGTCTTGGCCTTTACTATATATAGGACCCCTGGGGTGGGGTGAAAGGTATCCCATTCATTCTCCTACTCTCAGAGAATTCTCCCAAAGGCTCCCACAAGTCTCCAGCCTCTAGAAGTCTCAGAGTCATACCGGTGTAGCCATAGTGGTGGTGTTCGCACTAGTTCGTGATTAATTCACAAGCAGTTGGTGAATTTAATCTGAGTCGTCAACGAGAGTAAGTAATTTGTTACTTTATAAATTAGATTTAGATCTAATATTTTTGTATGCTTAGCCTTAATATATATTTAGAATAGTTCTAATATTATTGCCAATGTATTGGTATTTTAGATTAAACAAAATTAATTACGAATCGGTCCCAATCACGCTTCCGCTTTACGGAATTACGGAATTTCAATCCTTCATTGATTTGCTCTAATACTTCACCCATCACCCTTCAGAGATATTAATTACGAAGCAAGATTTCTCAAGTAAGTAAAATAATTTCACGGTCAGATAACGATCCTTGGATTAATGAAGCGATCGATAGTGTGAGGTTTATGGGGGATGTTGGCCAAATCGATTTACATTTTCTAGAGCATATTTTGCCACATTGCACTGTTGACCAATTGATGCATGTGGAGAAATGTTCAAAGGGAAGAGACCTCACTCCAGTAACCAACAAATTGTGGAAAAATTTCTACGAAAGAAAGTTCGGTAAAGATGATACTGATTTCGTAGTTGAGAGGACGCGACAGAAAAAGGAGACATTTAAATGGAAGCAATTGTATGAGGCAAAGATGAAGGAGTTGGAAAAGAAGGAGAGGGAAACTGGAAAGCGATTGATACAAAACTACCAGAAAGAAAAAGCTCGAAAACAAAGTCGTCAAATACAAATTCTTAAGGTTCCCTCTTTGAGAAATAAGAAACGAAGAAACTTTGCAGGAACAGGTGGCAACCGATTGAAGAAGGCAAAGAGAGGAGAACAAATTTGTGAGGTTTCCTCTTTGAGTAATAAGAAACGAAGCTTTGAAGGAACTGTTGGATTTGGATATAATATGAATAAGAGAAAGTTGTTGGAGAAGGCAAAGAGAGAAGTGGTTCAACTTGTAAACATACTACCACTCAAGGCTAGAAGATAG

mRNA sequence

ATGGGTGAGAGTGGCCAGTTCGCCGACTCAATAAGCCTACCATTTCAGGGGCAAGACCGAATGGGAGCTGGGAACATAACAGTACAAGATGGAATTCACTCCTTCCCCTATTGGGGTCCATTAGGTCCCACTGATAACGATCCTTGGATTAATGAAGCGATCGATAGTGTGAGGTTTATGGGGGATGTTGGCCAAATCGATTTACATTTTCTAGAGCATATTTTGCCACATTGCACTGTTGACCAATTGATGCATGTGGAGAAATGTTCAAAGGGAAGAGACCTCACTCCAGTAACCAACAAATTGTGGAAAAATTTCTACGAAAGAAAGTTCGGTAAAGATGATACTGATTTCGTAGTTGAGAGGACGCGACAGAAAAAGGAGACATTTAAATGGAAGCAATTGTATGAGGCAAAGATGAAGGAGTTGGAAAAGAAGGAGAGGGAAACTGGAAAGCGATTGATACAAAACTACCAGAAAGAAAAAGCTCGAAAACAAAGTCGTCAAATACAAATTCTTAAGGTTCCCTCTTTGAGAAATAAGAAACGAAGAAACTTTGCAGGAACAGGTGGCAACCGATTGAAGAAGGCAAAGAGAGGAGAACAAATTTGTGAGGTTTCCTCTTTGAGTAATAAGAAACGAAGCTTTGAAGGAACTGTTGGATTTGGATATAATATGAATAAGAGAAAGTTGTTGGAGAAGGCAAAGAGAGAAGTGGTTCAACTTGTAAACATACTACCACTCAAGGCTAGAAGATAG

Coding sequence (CDS)

ATGGGTGAGAGTGGCCAGTTCGCCGACTCAATAAGCCTACCATTTCAGGGGCAAGACCGAATGGGAGCTGGGAACATAACAGTACAAGATGGAATTCACTCCTTCCCCTATTGGGGTCCATTAGGTCCCACTGATAACGATCCTTGGATTAATGAAGCGATCGATAGTGTGAGGTTTATGGGGGATGTTGGCCAAATCGATTTACATTTTCTAGAGCATATTTTGCCACATTGCACTGTTGACCAATTGATGCATGTGGAGAAATGTTCAAAGGGAAGAGACCTCACTCCAGTAACCAACAAATTGTGGAAAAATTTCTACGAAAGAAAGTTCGGTAAAGATGATACTGATTTCGTAGTTGAGAGGACGCGACAGAAAAAGGAGACATTTAAATGGAAGCAATTGTATGAGGCAAAGATGAAGGAGTTGGAAAAGAAGGAGAGGGAAACTGGAAAGCGATTGATACAAAACTACCAGAAAGAAAAAGCTCGAAAACAAAGTCGTCAAATACAAATTCTTAAGGTTCCCTCTTTGAGAAATAAGAAACGAAGAAACTTTGCAGGAACAGGTGGCAACCGATTGAAGAAGGCAAAGAGAGGAGAACAAATTTGTGAGGTTTCCTCTTTGAGTAATAAGAAACGAAGCTTTGAAGGAACTGTTGGATTTGGATATAATATGAATAAGAGAAAGTTGTTGGAGAAGGCAAAGAGAGAAGTGGTTCAACTTGTAAACATACTACCACTCAAGGCTAGAAGATAG

Protein sequence

MGESGQFADSISLPFQGQDRMGAGNITVQDGIHSFPYWGPLGPTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYERKFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQIQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKRKLLEKAKREVVQLVNILPLKARR

Homology

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BLAST of Spg032894 vs. NCBI nr
Match: XP_038885873.1 (uncharacterized protein LOC120076176 [Benincasa hispida])

HSP 1 Score: 201.4 bits (511), Expect = 9.0e-48
Identity = 114/202 (56.44%), Postives = 138/202 (68.32%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           INEAIDS+RF+GDVG  DLH LE ILPHCTVDQLMH+E  SKGRDLTPVT+KLWKNFYE+
Sbjct: 18  INEAIDSLRFLGDVGDTDLHVLERILPHCTVDQLMHIENSSKGRDLTPVTDKLWKNFYEK 77

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFGK+D+D V+++ + KKE+FKWKQLYEAKM+ LEKK  E   R  QN QKE ARKQSR+
Sbjct: 78  KFGKNDSDLVIKKMKYKKESFKWKQLYEAKMEALEKKAMEIEARYKQNCQKENARKQSRK 137

Query: 170 IQILKVPSLRNKKRRNFAG--------TGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVG 229
           I   +  S  N K+R   G        T    LKK  R  Q+C+VSS         GT  
Sbjct: 138 IIFCEDVSSSNNKKRRSEGTIKSECNTTESKILKKPNREAQMCQVSS--------GGTTK 197

Query: 230 FGYNMNKRKLLEKAKREVVQLV 244
            G+   + K+L+KAKRE +Q +
Sbjct: 198 PGHRTKQSKILKKAKREALQCI 211

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BLAST of Spg032894 vs. NCBI nr
Match: XP_022979571.1 (uncharacterized protein LOC111479252 [Cucurbita maxima])

HSP 1 Score: 200.3 bits (508), Expect = 2.0e-47
Identity = 111/203 (54.68%), Postives = 141/203 (69.46%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+RF+GDVGQ DL+FLEHILPHCTVDQLMH+E CSKGRDLTP+TNKLWK FYER
Sbjct: 5   VNKAIDSLRFLGDVGQTDLNFLEHILPHCTVDQLMHIENCSKGRDLTPITNKLWKTFYER 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFGKDD + VV+R +  KE+F+WKQ+YE K+KELE K  E  KR I+N Q EKARKQSRQ
Sbjct: 65  KFGKDDVNCVVDRMKH-KESFRWKQMYELKIKELEDKAVEVEKRYIRNCQNEKARKQSRQ 124

Query: 170 IQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKR 229
           ++I ++                  LKK K   ++C+VSS +N KRS+ G           
Sbjct: 125 VKICEI------------SPSSKVLKKPKVHTKVCQVSSANNNKRSYLG--------GTS 184

Query: 230 KLLEKAKREVVQLVNILPLKARR 253
           K+L+KA++E +Q +    L A R
Sbjct: 185 KILKKARKETLQSIETKNLIAFR 186

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BLAST of Spg032894 vs. NCBI nr
Match: KAG6582214.1 (hypothetical protein SDJN03_22216, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 195.7 bits (496), Expect = 4.9e-46
Identity = 111/203 (54.68%), Postives = 140/203 (68.97%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+RF+GDVGQIDL+FLEHILPHCTVDQLMH+E CSKGRDLTPVTNKLWK FYE+
Sbjct: 5   VNKAIDSLRFLGDVGQIDLNFLEHILPHCTVDQLMHIENCSKGRDLTPVTNKLWKTFYEK 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFG+D  + V+ER +  KE+F+WKQ+YE KMKELE+K  E  KR IQN + EK RKQSRQ
Sbjct: 65  KFGEDGVNGVLERMKH-KESFRWKQMYELKMKELEEKAVEIEKRYIQNCRNEKNRKQSRQ 124

Query: 170 IQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKR 229
           ++I ++                  LKK K   +IC+VSS +N KRS+ G           
Sbjct: 125 VKICEI------------SPSSKVLKKPKVQTKICQVSSATNNKRSYGG--------GTS 184

Query: 230 KLLEKAKREVVQLVNILPLKARR 253
           K+L+KA++E  Q +    L A R
Sbjct: 185 KILKKARKETRQSIETKNLIAFR 186

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BLAST of Spg032894 vs. NCBI nr
Match: KAG6582215.1 (40S ribosomal protein S21-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 190.3 bits (482), Expect = 2.1e-44
Identity = 110/204 (53.92%), Postives = 139/204 (68.14%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+R +GDVGQ DL+FLEHILPHCTVDQLMH+E CSKGRDLTPVTNKLWK FYER
Sbjct: 5   VNKAIDSLRLLGDVGQTDLNFLEHILPHCTVDQLMHIENCSKGRDLTPVTNKLWKTFYER 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFG D  + V+ER +  KE+F+WKQ+YE KMK LE+K  E  KR IQN + EKARKQSRQ
Sbjct: 65  KFGIDAVNDVLERMKH-KESFRWKQMYELKMKGLEEKAVEIEKRYIQNCRNEKARKQSRQ 124

Query: 170 IQILKV-PSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNK 229
           ++I ++ PS                LKK +   ++C+VSS +N KRS+ G          
Sbjct: 125 VKICEILPS-------------SKVLKKPRVHTKVCQVSSANNNKRSYPGVTS------- 184

Query: 230 RKLLEKAKREVVQLVNILPLKARR 253
            K+L+KA++E  Q +    L A R
Sbjct: 185 -KILKKARKETRQSIETKNLIAFR 186

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BLAST of Spg032894 vs. NCBI nr
Match: XP_023526910.1 (uncharacterized protein LOC111790279 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.1 bits (479), Expect = 4.6e-44
Identity = 105/194 (54.12%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+RF+ DVG+ DL+FLEHILPHCTV+QLM +E CSKGRDLTPVTNKLWK FYER
Sbjct: 5   VNKAIDSLRFLEDVGETDLNFLEHILPHCTVEQLMRIENCSKGRDLTPVTNKLWKTFYER 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFGKD  + V+E  +  KE+FKWKQ+YE K KELE+K  E  KR IQN   EKARKQSRQ
Sbjct: 65  KFGKDAVNSVIEMMKH-KESFKWKQMYELKTKELEEKAVEIEKRYIQNCHNEKARKQSRQ 124

Query: 170 IQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKR 229
           ++I ++    +K            LKK K   ++C+VSS++N KRS+ G           
Sbjct: 125 VKICEISPPSSKV-----------LKKPKVQTKVCQVSSVTNNKRSYVG--------GTS 178

Query: 230 KLLEKAKREVVQLV 244
           K+L+KA++E +Q +
Sbjct: 185 KILKKARKETLQSI 178

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BLAST of Spg032894 vs. ExPASy TrEMBL
Match: A0A6J1IR58 (uncharacterized protein LOC111479252 OS=Cucurbita maxima OX=3661 GN=LOC111479252 PE=4 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 9.7e-48
Identity = 111/203 (54.68%), Postives = 141/203 (69.46%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+RF+GDVGQ DL+FLEHILPHCTVDQLMH+E CSKGRDLTP+TNKLWK FYER
Sbjct: 5   VNKAIDSLRFLGDVGQTDLNFLEHILPHCTVDQLMHIENCSKGRDLTPITNKLWKTFYER 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFGKDD + VV+R +  KE+F+WKQ+YE K+KELE K  E  KR I+N Q EKARKQSRQ
Sbjct: 65  KFGKDDVNCVVDRMKH-KESFRWKQMYELKIKELEDKAVEVEKRYIRNCQNEKARKQSRQ 124

Query: 170 IQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKR 229
           ++I ++                  LKK K   ++C+VSS +N KRS+ G           
Sbjct: 125 VKICEI------------SPSSKVLKKPKVHTKVCQVSSANNNKRSYLG--------GTS 184

Query: 230 KLLEKAKREVVQLVNILPLKARR 253
           K+L+KA++E +Q +    L A R
Sbjct: 185 KILKKARKETLQSIETKNLIAFR 186

[Close]

BLAST of Spg032894 vs. ExPASy TrEMBL
Match: A0A6J1ITM2 (uncharacterized protein LOC111479243 OS=Cucurbita maxima OX=3661 GN=LOC111479243 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 5.0e-44
Identity = 106/194 (54.64%), Postives = 134/194 (69.07%), Query Frame = 0


Query: 50  INEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKLWKNFYER 109
           +N+AIDS+RF+GDVGQ DL+FLEHILPHCTV+QLM +E  SKGRDLTPVTNKLWK FYER
Sbjct: 5   VNKAIDSLRFLGDVGQTDLNFLEHILPHCTVEQLMRIENSSKGRDLTPVTNKLWKTFYER 64

Query: 110 KFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEKARKQSRQ 169
           KFGKD  + V+E  +  KE+FKWKQ+YE K+KELE+K  E  KR IQN Q EKARKQSRQ
Sbjct: 65  KFGKDAVNGVLEMMKH-KESFKWKQMYELKIKELEEKAVEIEKRYIQNCQNEKARKQSRQ 124

Query: 170 IQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGFGYNMNKR 229
           ++I ++                  LKK+K   + C+VSS  N KRS+ G           
Sbjct: 125 VKICEI------------SPSSKVLKKSKVHTKFCQVSSAHNDKRSYLG--------GTS 177

Query: 230 KLLEKAKREVVQLV 244
           K+L+KA++E +Q +
Sbjct: 185 KILKKARKETLQSI 177

[Close]

BLAST of Spg032894 vs. ExPASy TrEMBL
Match: A0A5D3BGR1 (Transcription elongation factor B polypeptide 3 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold203G00180 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.5e-44
Identity = 108/212 (50.94%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 43  PTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKL 102
           P+  D  +N+AID++RF+GDVG+ D+H LE ILPHCTVDQLMHVEK S+GRDLTPVT+KL
Sbjct: 8   PSLVDLCVNKAIDNIRFLGDVGETDIHLLERILPHCTVDQLMHVEKSSEGRDLTPVTDKL 67

Query: 103 WKNFYERKFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEK 162
           WK FYER+FGK+ T  V+ER RQK+  F+W QLYEAKM+++EK E +   R+ Q+Y KE 
Sbjct: 68  WKKFYERQFGKESTTTVIERMRQKRVAFRWIQLYEAKMQDIEKNESKAADRIKQSYLKEN 127

Query: 163 ARKQSRQIQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGF 222
           ARKQSRQI                               QIC     S+ KRSF G+ G+
Sbjct: 128 ARKQSRQI-------------------------------QICSKVPPSSNKRSFGGS-GY 187

Query: 223 GYNM--NKRKLLEKAKREVVQLVNILPLKARR 253
           GYN+   K K+L+KAK EV+Q   +  +KA R
Sbjct: 188 GYNVANTKNKILKKAKIEVLQSQEMKNIKAWR 187

[Close]

BLAST of Spg032894 vs. ExPASy TrEMBL
Match: A0A1S4E1U0 (transcription elongation factor B polypeptide 3 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496337 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.5e-44
Identity = 108/212 (50.94%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 43  PTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKL 102
           P+  D  +N+AID++RF+GDVG+ D+H LE ILPHCTVDQLMHVEK S+GRDLTPVT+KL
Sbjct: 8   PSLVDLCVNKAIDNIRFLGDVGETDIHLLERILPHCTVDQLMHVEKSSEGRDLTPVTDKL 67

Query: 103 WKNFYERKFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEK 162
           WK FYER+FGK+ T  V+ER RQK+  F+W QLYEAKM+++EK E +   R+ Q+Y KE 
Sbjct: 68  WKKFYERQFGKESTTTVIERMRQKRVAFRWIQLYEAKMQDIEKNESKAADRIKQSYLKEN 127

Query: 163 ARKQSRQIQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGF 222
           ARKQSRQI                               QIC     S+ KRSF G+ G+
Sbjct: 128 ARKQSRQI-------------------------------QICSKVPPSSNKRSFGGS-GY 187

Query: 223 GYNM--NKRKLLEKAKREVVQLVNILPLKARR 253
           GYN+   K K+L+KAK EV+Q   +  +KA R
Sbjct: 188 GYNVANTKNKILKKAKIEVLQSQEMKNIKAWR 187

[Close]

BLAST of Spg032894 vs. ExPASy TrEMBL
Match: A0A1S3C4D6 (transcription elongation factor B polypeptide 3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496337 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 8.5e-44
Identity = 108/212 (50.94%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 43  PTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKL 102
           P+  D  +N+AID++RF+GDVG+ D+H LE ILPHCTVDQLMHVEK S+GRDLTPVT+KL
Sbjct: 8   PSLVDLCVNKAIDNIRFLGDVGETDIHLLERILPHCTVDQLMHVEKSSEGRDLTPVTDKL 67

Query: 103 WKNFYERKFGKDDTDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKEK 162
           WK FYER+FGK+ T  V+ER RQK+  F+W QLYEAKM+++EK E +   R+ Q+Y KE 
Sbjct: 68  WKKFYERQFGKESTTTVIERMRQKRVAFRWIQLYEAKMQDIEKNESKAADRIKQSYLKEN 127

Query: 163 ARKQSRQIQILKVPSLRNKKRRNFAGTGGNRLKKAKRGEQICEVSSLSNKKRSFEGTVGF 222
           ARKQSRQI                               QIC     S+ KRSF G+ G+
Sbjct: 128 ARKQSRQI-------------------------------QICSKVPPSSNKRSFGGS-GY 187

Query: 223 GYNM--NKRKLLEKAKREVVQLVNILPLKARR 253
           GYN+   K K+L+KAK EV+Q   +  +KA R
Sbjct: 188 GYNVANTKNKILKKAKIEVLQSQEMKNIKAWR 187

[Close]

BLAST of Spg032894 vs. TAIR 10
Match: AT2G42780.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcription; LOCATED IN: integral to membrane, nucleus; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: RNA polymerase II transcription factor SIII, subunit A (InterPro:IPR010684); Has 187 Blast hits to 186 proteins in 77 species: Archae - 0; Bacteria - 0; Metazoa - 104; Fungi - 29; Plants - 38; Viruses - 0; Other Eukaryotes - 16 (source: NCBI BLink). )

HSP 1 Score: 109.4 bits (272), Expect = 4.3e-24
Identity = 64/164 (39.02%), Postives = 98/164 (59.76%), Query Frame = 0


Query: 43  PTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKL 102
           P+  D  + +AID+V+++G VG +D   LE IL HCT++QL H+E  +   DL+P+T+K 
Sbjct: 11  PSLVDLCVRKAIDNVKYIGYVGGVDFQLLEQILQHCTLEQLKHIEDATDDTDLSPITDKF 70

Query: 103 WKNFYERKFGKDD-TDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKE 162
           WK FY++ +G++D  D + +    K   FKW+ LYE K+  +++KE+E G RL + Y+ E
Sbjct: 71  WKGFYKKHYGEEDMKDLIEDLEWNKVNDFKWRNLYELKVIAVQQKEKELGGRLKERYKDE 130

Query: 163 KARKQSRQIQILK--VPSLR-----NKKRRNFAGTGGNRLKKAK 199
             RKQSRQ ++     PS R     +    N      N +KKAK
Sbjct: 131 NERKQSRQTKVCSKAPPSKRPFWGNSASGYNLGHVKSNIMKKAK 174

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BLAST of Spg032894 vs. TAIR 10
Match: AT2G42780.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcription; LOCATED IN: integral to membrane, nucleus; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: RNA polymerase II transcription factor SIII, subunit A (InterPro:IPR010684). )

HSP 1 Score: 109.4 bits (272), Expect = 4.3e-24
Identity = 64/164 (39.02%), Postives = 98/164 (59.76%), Query Frame = 0


Query: 43  PTDNDPWINEAIDSVRFMGDVGQIDLHFLEHILPHCTVDQLMHVEKCSKGRDLTPVTNKL 102
           P+  D  + +AID+V+++G VG +D   LE IL HCT++QL H+E  +   DL+P+T+K 
Sbjct: 11  PSLVDLCVRKAIDNVKYIGYVGGVDFQLLEQILQHCTLEQLKHIEDATDDTDLSPITDKF 70

Query: 103 WKNFYERKFGKDD-TDFVVERTRQKKETFKWKQLYEAKMKELEKKERETGKRLIQNYQKE 162
           WK FY++ +G++D  D + +    K   FKW+ LYE K+  +++KE+E G RL + Y+ E
Sbjct: 71  WKGFYKKHYGEEDMKDLIEDLEWNKVNDFKWRNLYELKVIAVQQKEKELGGRLKERYKDE 130

Query: 163 KARKQSRQIQILK--VPSLR-----NKKRRNFAGTGGNRLKKAK 199
             RKQSRQ ++     PS R     +    N      N +KKAK
Sbjct: 131 NERKQSRQTKVCSKAPPSKRPFWGNSASGYNLGHVKSNIMKKAK 174

The following BLAST results are available for this feature:

BLAST of Spg032894 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885873.1	9.0e-48	56.44	uncharacterized protein LOC120076176 [Benincasa hispida]	[more]
XP_022979571.1	2.0e-47	54.68	uncharacterized protein LOC111479252 [Cucurbita maxima]	[more]
KAG6582214.1	4.9e-46	54.68	hypothetical protein SDJN03_22216, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6582215.1	2.1e-44	53.92	40S ribosomal protein S21-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526910.1	4.6e-44	54.12	uncharacterized protein LOC111790279 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg032894 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg032894 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IR58	9.7e-48	54.68	uncharacterized protein LOC111479252 OS=Cucurbita maxima OX=3661 GN=LOC111479252...	[more]
A0A6J1ITM2	5.0e-44	54.64	uncharacterized protein LOC111479243 OS=Cucurbita maxima OX=3661 GN=LOC111479243...	[more]
A0A5D3BGR1	8.5e-44	50.94	Transcription elongation factor B polypeptide 3 isoform X2 OS=Cucumis melo var. ...	[more]
A0A1S4E1U0	8.5e-44	50.94	transcription elongation factor B polypeptide 3 isoform X2 OS=Cucumis melo OX=36...	[more]
A0A1S3C4D6	8.5e-44	50.94	transcription elongation factor B polypeptide 3 isoform X1 OS=Cucumis melo OX=36...	[more]

BLAST of Spg032894 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42780.1	4.3e-24	39.02	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcripti...	[more]
AT2G42780.2	4.3e-24	39.02	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of transcripti...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 133..161
None	No IPR available	GENE3D	6.10.250.3180		coord: 56..164 e-value: 1.1E-21 score: 78.8
None	No IPR available	PANTHER	PTHR47543	OS08G0169600 PROTEIN	coord: 47..192
IPR010684	RNA polymerase II transcription factor SIII, subunit A	PFAM	PF06881	Elongin_A	coord: 61..170 e-value: 8.4E-26 score: 90.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg032894.1	Spg032894.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006368	transcription elongation from RNA polymerase II promoter
cellular_component	GO:0070449	elongin complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:1901363	heterocyclic compound binding
molecular_function	GO:0097159	organic cyclic compound binding
molecular_function	GO:0016462	pyrophosphatase activity