Spg031615 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031615
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Location	scaffold11: 37780923 .. 37784726 (-)
RNA-Seq Expression	Spg031615
Synteny	Spg031615

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAAACGGATAAAATTCAAAGTTCATCTAAAATTCCTATTTTTAAATTCGATTGATTTTATCCATTCGAGATAAAACCAAACAAAATAAAAAAAATAAAAAAGGAAATAGGCACGTGCGAACCCAAACGAAAATACACCAACTGGCAACTCCGCCAAAAAGCTTTCATTCAATTCAATCGAATTCTTCTTCTTCTTCTTCCTCCTCCAAGCTCTTCACAATTGCAACAACTTCCATAATTTCCTTTGATCTTGCACGAGCAGGTTGCTAGTGATGTCGATTCTTATGATTGGCAGGAGGAAGATTTGTCCGAAGAGTGCTTGGGCCGCCGGTCGCCGGTTACTTGCTCCGATCACCTCCCAAGGTCCGGCGGCAACTGGGAGATTGAAGCCCAATTCGATTCCAGAGCCATCTCCTCAGCTTGCATGCCCTAGTTTCTCTAGAGCGTGGCTTCATTCTCCTGCTAATCGCTTCGAGGTGAGTTCGACGGTTGGATTTTTCTGCTCTGGTTGTCGTTTTCCTTCTGTTGTTAGCATTCGTTCTTGTGATCTTAAGACGAAAGCGCCATTTCTATTTTTTGGATTCTGAGATAGCAGCGGTGTCGATGATTCTATGTTTTGGTTGTGTCTGATATTGTGCCGATTTCAGCGAAGTCCTTCTCCTTGTAATTATCTGGGCAAACTAAGAAATGAGGTATTTGTGAATGTAATTACGGAATTGGTTCGTTTTGTAGGCGAGTATAAGAGGCGTTTCGAAGATATGTCGCTTTTCTACCCAGGCTTTGGATGGTCTGCCAGTTTGTAGGTTTGTGGATGTACCTTTGGCTCAAACTGGTGAAGGGATTGCTGAATGTGAGCTTCTTAAATGGTTTGTGCAGGAGGTGAGGCCAACAATACTTTTTCGGTTTTTCCCCTTTTCATTAGGGCTCTTTCTTTCTGTTTTTATTTATTTACTTTTAGTGTTTAACATTTTAAGATATCCCTCATGAAAATGAATGAGATTTCGTTGATTTCTTCAAACATTTGTGTTATGTTCTGAGCTCTAACTGCTGGACATTGCTGTCATGAGCGAACTGTTGGCTTTTTGTCTGGTTTGCTGAGGTTGCAAGATTAGTTCAAGTGATTGCAACTCTGGAAGTTAATTGATTGGACTATTTCTTATCAAATGTAGATGAATATTGATATTTAATCTTTTGGCAGGGGGAAGAAGTTGAAGAGTTTCAACCACTTTGTGAAGTTCAGAGTGACAAAGCAACAATTGAAATAACTAGTCGCTACAAAGGAAAAGTTGGTCAACTTCTATATGTTCCGGGTGATATTGTGAAGGTGAGATTTCAACCCCCAATAGTCATGAATATGATGAACTCATGGAAACTTTTGTTGCCTGTTGATGGAGAGTAGAAAAAATCTTCATGTTTTGAAATGTGAATGCCTATTTTCTGGATAATTATTAAGTTGCTGATATATCAAATGAACAGGTCCTTAAATGTTTTATCACCTTTACGTTAAAATGTTTTAGCTTGTAGGTTTCTTGCTGATGTTTGATTCTTTTATGCTGTCATTTTCAACCTTGATACTGTTTCTTCTCTTATACTATTTGCGAATTGAACACTGCCTAATTGGTAGGAAACAACATTGTCCATTCGTGCAGAAGCACTTTCTGGGTAATTAATCATAACATCACATTCTAGAGATTAAACAGAAAAGATACTTATGTTTTGTCAAATGTTATCACAGGTTGGAGAAACTCTTTTAAAAGTAATTGTTGATGATTTGGAGGATGAAACCCAGTTTTCAGGATTGACCAAAGAACAATTGGCTAAACCTGAATTAGTAGATTATCAAGTGGACAAACATAAAAGTGGTGGAGTTCTATCCACTCCACCTATCCGCAATCTTGCAAAACAATATGGTATAGATATAAATGATGTCCGTGGGACTGGTGTAGATGGCAGGGTGTTAAAAGAAGATATTCTTCAGTATGCTGTTGAAAAGGGAATTCTTGAAGAACAGTCTGTTTGTCCTGCTGCTAGTCCTAGAGAACAACTTGGCGGATCAGAGAATTACCCTCATACACCAGATGAAGTTAGGTGGAGCTACGAGGACAAGAAAGTTCAATTGAGGTATAAATTTGGTGTCCGTTACTTGTAAGGAACTATTAACTAGGCCAATAGAATCATGCAATGTGGCACTGGTACTACATATCTTTTGACTGTTAAAAAACAACTCCTGCTCTCTTGTTTTCTGCCCACTTGGAACAACTAAGTATGCCTTAAAGTTTGATTTCAACTGGCAAAATGATTATTTTCTTTGCTATGCGCTAGTTTTGTATAACTTTGTTGTGTCGTTTGCTGGATTTAGAGTTATATGCACCTGATATGTGCTTTGTTTTCAGAGGATTCCAACGAGCAATGGTTAAATCTATGACCATTGCTGCAAAAGTTCCCCATTTTCACTATGTAGAGGAGATCAATTGTGATGCACTTGTGGAACTTAAAGCATCGTTCCAAGGAAATACTACCGAACCAGATGTCAAGCACACTTTTCTTCCATTATTGATCAAGTCGCTCTCTATGGCAATGGGCAAATATCCAATGCTAAATAGTTGCTTCAATGAAGAGTCATTTGAGGTCACTCTCAAAGGTACGTAGTCGGAGATGTTGATAATATCTTTTTAAACTTGAATTCCTTCCTTGTTCTTATTTAAATTACAAATACATTTATTTTTGCTTGTTGTAGGCTCCCATAATATTGGAATTGCCATGGCTACTCCACATGGTTTGGTTGTGCCAAATATAAAGAATGTGCAGTCCCTTTCGGTATTGGAGGTTCGTAAATCACCACCCACGTATCCTATTTTGCTTAAATGAAACCAGAGGATCATTTTGGAGTTCTCTAGTTTAATAATTGAGTTCAGGAGGTTGATTGCTGATCTATTTAAATCTATAACATTGAAGTAATGGATACTTGTTACGGTTTGATTTCTGTTTCCATCTGTAATTCTCGCCAGTCTTCTGTTCCTTCAACCGTCGTTTACAAATTTCCTTTTCATTTTATAGAATTCTTAAACAATCGTCAACCTTTGTCATTGTTAGTGGCATGTCTGCCTTTCACTGAAGACTTCTCTTTCAAATGCATTTGCTTAGATCACAAAGGAACTTTCACGCTTACAACAGCTATCCATGGATAACAAGCTCAGTCCTGGGGATGTATCCGGCGGCACAATAACCTTAAGCAATATTGGAGCTATTGGTGGGAAATTCGGTTCCCCTCTGCTCAACTTGCCTGAAGTAGCTATCATTGCAATGGGCCGAATCAAAAAGGTTCCACAGATTGCTGATGATGGGGACGTGTACCCCGCATCGATCATGACGGTGAGCAACCTGAAACCAATCTTTACTTCCTCCAAATTAAATGTCTATATCGGTCGTGACTGACCCGACCCCTTTCTTCTCAGGTGAACATTGGTGCAGACCATAGAGTTTTGGATGGAGCAACAGTGGCCAAATTCTGCAACGAATGGAAGCGATTCATTGAAAATCCAGACCTTCTCATCTTGCAAATGAGATGAGCTGATCTTCAGCTAAGTAGATATACCAATATCCCTTGATATAATAATAAATCAAATGGTCAGCATGTGCAAGAATAATAGATATTATTTTCAAATGTCAATAAAGATCGAATCGATAAACTTGTCCCTCTTTGAGCAAAGCTACATGGTTACGTAACGTAATCAAATTTGATTGCAATTGTAAGATTACGCGGCTTCTAATGATAATGCTAACAAGGAAATTTTGAGACTTG

mRNA sequence

ATGTCGATTCTTATGATTGGCAGGAGGAAGATTTGTCCGAAGAGTGCTTGGGCCGCCGGTCGCCGGTTACTTGCTCCGATCACCTCCCAAGGTCCGGCGGCAACTGGGAGATTGAAGCCCAATTCGATTCCAGAGCCATCTCCTCAGCTTGCATGCCCTAGTTTCTCTAGAGCGTGGCTTCATTCTCCTGCTAATCGCTTCGAGGTTGGAGAAACTCTTTTAAAAGTAATTGTTGATGATTTGGAGGATGAAACCCAGTTTTCAGGATTGACCAAAGAACAATTGGCTAAACCTGAATTAGTAGATTATCAAGTGGACAAACATAAAAGTGGTGGAGTTCTATCCACTCCACCTATCCGCAATCTTGCAAAACAATATGGTATAGATATAAATGATGTCCGTGGGACTGGTGTAGATGGCAGGGTGTTAAAAGAAGATATTCTTCAGTATGCTGTTGAAAAGGGAATTCTTGAAGAACAGTCTGTTTGTCCTGCTGCTAGTCCTAGAGAACAACTTGGCGGATCAGAGAATTACCCTCATACACCAGATGAAGTTAGGTGGAGCTACGAGGACAAGAAAGTTCAATTGAGAGGATTCCAACGAGCAATGGTTAAATCTATGACCATTGCTGCAAAAGTTCCCCATTTTCACTATGTAGAGGAGATCAATTGTGATGCACTTGTGGAACTTAAAGCATCGTTCCAAGGAAATACTACCGAACCAGATGTCAAGCACACTTTTCTTCCATTATTGATCAAGTCGCTCTCTATGGCAATGGGCAAATATCCAATGCTAAATAGTTGCTTCAATGAAGAGTCATTTGAGGTCACTCTCAAAGGCTCCCATAATATTGGAATTGCCATGGCTACTCCACATGGTTTGGTTGTGCCAAATATAAAGAATGTGCAGTCCCTTTCGGTATTGGAGATCACAAAGGAACTTTCACGCTTACAACAGCTATCCATGGATAACAAGCTCAGTCCTGGGGATGTATCCGGCGGCACAATAACCTTAAGCAATATTGGAGCTATTGGTGGGAAATTCGGTTCCCCTCTGCTCAACTTGCCTGAAGTAGCTATCATTGCAATGGGCCGAATCAAAAAGGTTCCACAGATTGCTGATGATGGGGACGTGTACCCCGCATCGATCATGACGGTGAACATTGGTGCAGACCATAGAGTTTTGGATGGAGCAACAGTGGCCAAATTCTGCAACGAATGGAAGCGATTCATTGAAAATCCAGACCTTCTCATCTTGCAAATGAGATGA

Coding sequence (CDS)

ATGTCGATTCTTATGATTGGCAGGAGGAAGATTTGTCCGAAGAGTGCTTGGGCCGCCGGTCGCCGGTTACTTGCTCCGATCACCTCCCAAGGTCCGGCGGCAACTGGGAGATTGAAGCCCAATTCGATTCCAGAGCCATCTCCTCAGCTTGCATGCCCTAGTTTCTCTAGAGCGTGGCTTCATTCTCCTGCTAATCGCTTCGAGGTTGGAGAAACTCTTTTAAAAGTAATTGTTGATGATTTGGAGGATGAAACCCAGTTTTCAGGATTGACCAAAGAACAATTGGCTAAACCTGAATTAGTAGATTATCAAGTGGACAAACATAAAAGTGGTGGAGTTCTATCCACTCCACCTATCCGCAATCTTGCAAAACAATATGGTATAGATATAAATGATGTCCGTGGGACTGGTGTAGATGGCAGGGTGTTAAAAGAAGATATTCTTCAGTATGCTGTTGAAAAGGGAATTCTTGAAGAACAGTCTGTTTGTCCTGCTGCTAGTCCTAGAGAACAACTTGGCGGATCAGAGAATTACCCTCATACACCAGATGAAGTTAGGTGGAGCTACGAGGACAAGAAAGTTCAATTGAGAGGATTCCAACGAGCAATGGTTAAATCTATGACCATTGCTGCAAAAGTTCCCCATTTTCACTATGTAGAGGAGATCAATTGTGATGCACTTGTGGAACTTAAAGCATCGTTCCAAGGAAATACTACCGAACCAGATGTCAAGCACACTTTTCTTCCATTATTGATCAAGTCGCTCTCTATGGCAATGGGCAAATATCCAATGCTAAATAGTTGCTTCAATGAAGAGTCATTTGAGGTCACTCTCAAAGGCTCCCATAATATTGGAATTGCCATGGCTACTCCACATGGTTTGGTTGTGCCAAATATAAAGAATGTGCAGTCCCTTTCGGTATTGGAGATCACAAAGGAACTTTCACGCTTACAACAGCTATCCATGGATAACAAGCTCAGTCCTGGGGATGTATCCGGCGGCACAATAACCTTAAGCAATATTGGAGCTATTGGTGGGAAATTCGGTTCCCCTCTGCTCAACTTGCCTGAAGTAGCTATCATTGCAATGGGCCGAATCAAAAAGGTTCCACAGATTGCTGATGATGGGGACGTGTACCCCGCATCGATCATGACGGTGAACATTGGTGCAGACCATAGAGTTTTGGATGGAGCAACAGTGGCCAAATTCTGCAACGAATGGAAGCGATTCATTGAAAATCCAGACCTTCTCATCTTGCAAATGAGATGA

Protein sequence

MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAATGRLKPNSIPEPSPQLACPSFSRAWLHSPANRFEVGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQMR

Homology

[Close]

BLAST of Spg031615 vs. NCBI nr
Match: XP_022137797.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Momordica charantia])

HSP 1 Score: 659.4 bits (1700), Expect = 2.0e-185
Identity = 354/519 (68.21%), Postives = 377/519 (72.64%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MS++MIGRRKI P SAWAAGRRLL+P  SQG   +         P SIPEPSP LAC  F
Sbjct: 1   MSMIMIGRRKISPGSAWAAGRRLLSPFASQGSFPSLFQLPLEQTPKSIPEPSPPLACSRF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
           S  WL SPANRFE                                               
Sbjct: 61  STTWLQSPANRFEANRRGVSKICGFSSQALDGLPVFRLVDVPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV VDD ED 
Sbjct: 121 KEGDLVEAFQPLCEVQSDKATIEITSRYKGKVGQLLYVPGDIVKVGETLLKVGVDDFEDG 180

Query: 181 TQFSGLTKEQLAKPEL-VDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVL 240
           TQ SG T+  LA PEL +D Q+DK+K GGVLSTP +R+LAKQY IDINDVRGTG DGRVL
Sbjct: 181 TQVSGPTEGHLANPELTIDIQLDKNKCGGVLSTPAVRDLAKQYAIDINDVRGTGTDGRVL 240

Query: 241 KEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAM 300
           KEDILQYAVEKGILEEQ V  AASPREQ  GSE+  H PDEVRWSYEDKKVQLRGFQRAM
Sbjct: 241 KEDILQYAVEKGILEEQHVSSAASPREQFDGSESRTHAPDEVRWSYEDKKVQLRGFQRAM 300

Query: 301 VKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYP 360
           VKSMTIAAKVPHFHYVEEINCD L+ELKASFQGNT EPDVKHT LPLLIKSLSMAM KYP
Sbjct: 301 VKSMTIAAKVPHFHYVEEINCDGLLELKASFQGNTPEPDVKHTSLPLLIKSLSMAMNKYP 360

Query: 361 MLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMD 420
           MLNSCFNEES+EVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSV+EITKELSRLQQL++D
Sbjct: 361 MLNSCFNEESYEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVMEITKELSRLQQLALD 420

Query: 421 NKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASI 423
           NKL PGD+SGGTITLSNIGAIGGKFGSPLLNLPEVAIIA+GRIKK+PQI+DDGDVYPASI
Sbjct: 421 NKLGPGDISGGTITLSNIGAIGGKFGSPLLNLPEVAIIAIGRIKKIPQISDDGDVYPASI 480

[Close]

BLAST of Spg031615 vs. NCBI nr
Match: XP_022973737.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita maxima])

HSP 1 Score: 655.2 bits (1689), Expect = 3.8e-184
Identity = 352/518 (67.95%), Postives = 378/518 (72.97%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MSILMI RRKI P SAWAAGRRLL P+  QGP+A+         P S+PEP   LACPSF
Sbjct: 1   MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACPSF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            R  LHSPANRFE                                               
Sbjct: 61  CRPGLHSPANRFEASIKGVSKICRFSSQALDGLPVFRTVDVPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV+VD+ ED 
Sbjct: 121 KEGEEVEEFQPICEVQSDKATIEITSRYKGKVSRLLYVPGDIVKVGETLLKVLVDEFEDG 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
           TQ SGLT+E LAKPELVD Q+DK K  GVLSTP +RNLAKQY IDINDVRGTG DGR+LK
Sbjct: 181 TQVSGLTEEHLAKPELVDSQLDKSKGVGVLSTPAVRNLAKQYDIDINDVRGTGTDGRILK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           +DILQYAV+KGI+EEQ V  A SP+EQL  SENYP+  DEV WSYEDKKVQLRG+QRAMV
Sbjct: 241 DDILQYAVKKGIIEEQRVSSAPSPKEQLVESENYPNAQDEVWWSYEDKKVQLRGYQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSMTI+AKVPHFHYVEEINCD LVELKA FQ NTT+PDVKHTFLPLLIKSLSMAM KYPM
Sbjct: 301 KSMTISAKVPHFHYVEEINCDGLVELKACFQENTTKPDVKHTFLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           +NSCFNEESFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSRLQ+L+MDN
Sbjct: 361 VNSCFNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSRLQRLAMDN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSP DVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQI +DGDVYPASIM
Sbjct: 421 KLSPVDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQITEDGDVYPASIM 480

[Close]

BLAST of Spg031615 vs. NCBI nr
Match: XP_038883446.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 651.0 bits (1678), Expect = 7.2e-183
Identity = 342/469 (72.92%), Postives = 373/469 (79.53%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MSIL IGRR+I P++AWA GRRLL+P T QG +A+         P S+P+P PQLA  S 
Sbjct: 1   MSILTIGRRRISPRTAWATGRRLLSPFTYQGSSASISQLPSDQTPKSLPQPFPQLASASS 60

Query: 61  SRAWLHSPANRFE------------------------------------------VGETL 120
            RAWLHS ANRFE                                          VGETL
Sbjct: 61  GRAWLHSHANRFEGDEVEEFQPLCEVQSDKATIEITSRYKGKVGQLLYVPGDIVKVGETL 120

Query: 121 LKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDV 180
           LKV VD  E+E Q SGLT+  LAKPE+++ Q D++K  GVLSTPP+R+LAKQ+GIDINDV
Sbjct: 121 LKVFVDGCENEIQVSGLTEGHLAKPEVIETQQDRNKICGVLSTPPVRDLAKQHGIDINDV 180

Query: 181 RGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKK 240
            GTG DGRVLKED+LQYAVEKGILEEQ V  AA  REQ   SEN+ + PD VRWS EDKK
Sbjct: 181 CGTGTDGRVLKEDVLQYAVEKGILEEQ-VSSAAGSREQFDESENHTNAPDGVRWSSEDKK 240

Query: 241 VQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIK 300
           +QLRGFQRAMVKSMT AAKVPHFHYVEEINCDAL+ELKASFQGNTTEP+VKHT LPLLIK
Sbjct: 241 IQLRGFQRAMVKSMTNAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIK 300

Query: 301 SLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKE 360
           SLS+AM KYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKE
Sbjct: 301 SLSVAMSKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKE 360

Query: 361 LSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIA 420
           LSRLQ L+MDNKLSPGD+SGGTITLSNIGAIGGKFGSPLLNLPEV+IIA+GRIKKVPQIA
Sbjct: 361 LSRLQLLAMDNKLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQIA 420

Query: 421 DDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQMR 423
           DDGDVYP SIMTVN+GADHRVLDGA VAKFCNEWKRFIENP+LLIL MR
Sbjct: 421 DDGDVYPTSIMTVNVGADHRVLDGAAVAKFCNEWKRFIENPELLILHMR 468

[Close]

BLAST of Spg031615 vs. NCBI nr
Match: XP_022947515.1 (lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita moschata] >KAG7028992.1 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.0 bits (1673), Expect = 2.7e-182
Identity = 349/518 (67.37%), Postives = 377/518 (72.78%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MSILMI RRKI P S+WAAG RLL P+  QGP+A+         P S+PEP   LACPSF
Sbjct: 1   MSILMISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACPSF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            R  LHSPANRFE                                               
Sbjct: 61  CRPGLHSPANRFEASIKGVSKVCRFSSQALDGLPVFRTVDVLLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLK++VD+ E+ 
Sbjct: 121 KEGEEVEEFQPICEVQSDKATIEITSRYKGKVSRLLYVPGDIVKVGETLLKLLVDEFEEG 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
           T  SGLT+E LAKPELVD Q+DK K  GVLSTP +RNLAKQY IDINDVRGTG DGR+LK
Sbjct: 181 TPVSGLTEEHLAKPELVDSQLDKSKGIGVLSTPAVRNLAKQYDIDINDVRGTGTDGRILK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           +DILQYAV+KGI+EEQ V  A SP+EQL  SENYP+  DEVRWSYEDKKVQLRG+QRAMV
Sbjct: 241 DDILQYAVKKGIIEEQRVSSAPSPKEQLVESENYPNAQDEVRWSYEDKKVQLRGYQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSMTI+AKVPHFHYVEEINCD LVELKA FQ NTTEPDVKHTFLPLLIKSLSMAM KYPM
Sbjct: 301 KSMTISAKVPHFHYVEEINCDGLVELKACFQENTTEPDVKHTFLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           +NSCFNEESFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSRLQ+L+MDN
Sbjct: 361 VNSCFNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSRLQRLAMDN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSP DVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQI +DGDVYPASIM
Sbjct: 421 KLSPVDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQITEDGDVYPASIM 480

[Close]

BLAST of Spg031615 vs. NCBI nr
Match: KAG6597534.1 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 641.0 bits (1652), Expect = 7.4e-180
Identity = 344/514 (66.93%), Postives = 372/514 (72.37%), Query Frame = 0


Query: 5   MIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSFSRAW 64
           MI RRKI P S+WAAG RLL P+  QGP+A+         P S+PEP   LACPSF R  
Sbjct: 1   MISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACPSFCRPG 60

Query: 65  LHSPANRFE--------------------------------------------------- 124
           LHSPANRFE                                                   
Sbjct: 61  LHSPANRFEASIKGVSKVCRFSSQALDGLPVFRTVDVPLAQTGEGIAECELLKWFVKEGE 120

Query: 125 ----------------------------------------VGETLLKVIVDDLEDETQFS 184
                                                   VGETLLK++VD+ E+ T  S
Sbjct: 121 EVEEFQPICEVQSDKATIEITSRYKGKVSRLLYVPGDIVKVGETLLKLLVDEFEEGTPVS 180

Query: 185 GLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLKEDIL 244
           GLT+E LAKPELVD Q+DK K  GVLSTP +RNLAKQY IDINDV GTG DGR+LK+DIL
Sbjct: 181 GLTEEHLAKPELVDSQLDKSKGVGVLSTPAVRNLAKQYDIDINDVHGTGTDGRILKDDIL 240

Query: 245 QYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMVKSMT 304
           QYAV+KGI+EEQ V  A SP+EQL  SENYP+  DEVRWSYEDKKVQLRG+QRAMVKSMT
Sbjct: 241 QYAVKKGIIEEQRVSSAPSPKEQLVESENYPNAQDEVRWSYEDKKVQLRGYQRAMVKSMT 300

Query: 305 IAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPMLNSC 364
           I+AKVPHFHYVEEINCD LVELKA FQ NTTEPDVKHTFLPLLIKSLSMAM KYPM+NSC
Sbjct: 301 ISAKVPHFHYVEEINCDGLVELKACFQENTTEPDVKHTFLPLLIKSLSMAMSKYPMVNSC 360

Query: 365 FNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDNKLSP 423
           FNEESFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSRLQ+L+MDNKLSP
Sbjct: 361 FNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSRLQRLAMDNKLSP 420

[Close]

BLAST of Spg031615 vs. ExPASy Swiss-Prot
Match: Q9M7Z1 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCE2 PE=1 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 4.7e-121
Identity = 224/362 (61.88%), Postives = 274/362 (75.69%), Query Frame = 0


Query: 61  HSPANRFEVGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIR 120
           HSP +  +VGETL+++ V+D +D    S LT +      L   +       G LSTP +R
Sbjct: 134 HSPGDIIKVGETLVRLAVEDSQD----SLLTTDSSEIVTLGGSKQGTENLLGALSTPAVR 193

Query: 121 NLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPH 180
           NLAK  GIDIN + GTG DGRVLKED+L+++ +KG + +      +S    +GG      
Sbjct: 194 NLAKDLGIDINVITGTGKDGRVLKEDVLRFSDQKGFVTDS----VSSEHAVIGGDS---- 253

Query: 181 TPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTE 240
              +   ++EDK V LRGF RAMVK+MT+A  VPHFH+VEEINCD+LVELK  F+ N T+
Sbjct: 254 VSTKASSNFEDKTVPLRGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTD 313

Query: 241 PDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIK 300
             +KHTFLP LIKSLSMA+ KYP +NSCFN ES E+ LKGSHNIG+AMAT HGLVVPNIK
Sbjct: 314 STIKHTFLPTLIKSLSMALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIK 373

Query: 301 NVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAI 360
           NVQSLS+LEITKELSRLQ L+ +NKL+P DV+GGTITLSNIGAIGGKFGSPLLNLPEVAI
Sbjct: 374 NVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAI 433

Query: 361 IAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQ 420
           IA+GRI+KVP+ + +G VYPASIM VNI ADHRVLDGATVA+FC +WK ++E P+LL+LQ
Sbjct: 434 IALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQ 483

Query: 421 MR 423
           MR
Sbjct: 494 MR 483

[Close]

BLAST of Spg031615 vs. ExPASy Swiss-Prot
Match: P11182 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Homo sapiens OX=9606 GN=DBT PE=1 SV=3)

HSP 1 Score: 263.1 bits (671), Expect = 5.5e-69
Identity = 146/369 (39.57%), Postives = 226/369 (61.25%), Query Frame = 0


Query: 69  VGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKH---KSGGVLSTPPIRNLAKQ 128
           VG+ L+ +  + L+D       ++E + +   V +    H   K    L+TP +R LA +
Sbjct: 131 VGKPLVDIETEALKD-------SEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAME 190

Query: 129 YGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQ--SVCPAASPREQLGGSENYPHTPD 188
             I +++V G+G DGR+LKEDIL Y      LE+Q  ++ P  SP+ ++      P  P 
Sbjct: 191 NNIKLSEVVGSGKDGRILKEDILNY------LEKQTGAILP-PSPKVEIMPP---PPKPK 250

Query: 189 EVR----------WSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKAS 248
           ++           ++ +DK   ++GFQ+AMVK+M+ A K+PHF Y +EI+   LV+L+  
Sbjct: 251 DMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREE 310

Query: 249 FQGNTTEPDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHG 308
            +       +K +F+P  +K+ S+ + ++P+LN+  +E    +T K SHNIGIAM T  G
Sbjct: 311 LKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQG 370

Query: 309 LVVPNIKNVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLL 368
           L+VPN+KNVQ  S+ +I  EL+RLQ+L    +LS  D++GGT TLSNIG+IGG F  P++
Sbjct: 371 LIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVI 430

Query: 369 NLPEVAIIAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIEN 423
             PEVAI A+G IK +P+    G+VY A IM V+  ADHRV+DGAT+++F N WK ++EN
Sbjct: 431 MPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLEN 482

[Close]

BLAST of Spg031615 vs. ExPASy Swiss-Prot
Match: P53395 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Mus musculus OX=10090 GN=Dbt PE=1 SV=2)

HSP 1 Score: 260.4 bits (664), Expect = 3.6e-68
Identity = 143/360 (39.72%), Postives = 219/360 (60.83%), Query Frame = 0


Query: 69  VGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKH---KSGGVLSTPPIRNLAKQ 128
           VG+ L+ +  + L+D       ++E + +   V +    H   K    L+TP +R LA +
Sbjct: 131 VGKPLIDIETEALKD-------SEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRLAME 190

Query: 129 YGIDINDVRGTGVDGRVLKEDILQYAVEK-GILEEQSVCPAASPREQLGGSENYPHTP-- 188
             I +++V G+G DGR+LKEDIL +  ++ G +   S     +P         +P TP  
Sbjct: 191 NNIKLSEVVGSGKDGRILKEDILSFLEKQTGAILPPSPKSEITPPPPQPKDRTFP-TPIA 250

Query: 189 DEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPD 248
               ++ +D+   + GFQ+AMVK+M+ A K+PHF Y +EI+   LV+L+   +       
Sbjct: 251 KPPVFTGKDRTEPVTGFQKAMVKTMSAALKIPHFGYCDEIDLTQLVKLREELKPVALARG 310

Query: 249 VKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNV 308
           +K +F+P  +K+ S+ + ++P+LN+  +E    +T K SHNIGIAM T  GL+VPN+KNV
Sbjct: 311 IKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTELGLIVPNVKNV 370

Query: 309 QSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIA 368
           Q  SV EI  EL+RLQ+L    +L   D++GGT TLSNIG+IGG +  P++  PEVAI A
Sbjct: 371 QVRSVFEIAMELNRLQKLGSSGQLGTTDLTGGTFTLSNIGSIGGTYAKPVILPPEVAIGA 430

Query: 369 MGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQMR 423
           +G IK +P+    GDVY A IM V+  ADHRV+DGAT+++F N WK ++ENP  ++L ++
Sbjct: 431 LGAIKALPRFDQKGDVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK 482

[Close]

BLAST of Spg031615 vs. ExPASy Swiss-Prot
Match: Q23571 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=dbt-1 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.0e-67
Identity = 147/373 (39.41%), Postives = 216/373 (57.91%), Query Frame = 0


Query: 61  HSPANRFEVGETLLKV----IVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLST 120
           H       VG+ L+ V     V++ E   + +  +  +  K          H  G VL+T
Sbjct: 89  HEVDGMARVGQALIDVEIEGNVEEPEQPKKEAASSSPEAPKSSAPKAPESAHSEGKVLAT 148

Query: 121 PPIRNLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSE 180
           P +R +A +  I + +VRGTG DGRVLKED+L++                 P +   GS 
Sbjct: 149 PAVRRIAIENKIKLAEVRGTGKDGRVLKEDVLKFL-------------GQVPADHTSGST 208

Query: 181 NYPHT---PDEVRWSY----EDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVE 240
           N   T   P     SY    ED  V +RG+ RAMVK+MT A K+PHF Y +EIN D+LV+
Sbjct: 209 NIRTTHQAPQPSSKSYEPLKEDVAVPIRGYTRAMVKTMTEALKIPHFGYNDEINVDSLVK 268

Query: 241 LKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMA 300
            +A  +    E  +K +++P  IK+ S+A+ +YP LNS  +E+   V  K SHNI +AM 
Sbjct: 269 YRAELKEFAKERHIKLSYMPFFIKAASLALLEYPSLNSTTDEKMENVIHKASHNICLAMD 328

Query: 301 TPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFG 360
           TP GLVVPNIKN +  S+ EI +EL+RL +     ++   D+  GT +LSNIG IGG + 
Sbjct: 329 TPGGLVVPNIKNCEQRSIFEIAQELNRLLEAGKKQQIKREDLIDGTFSLSNIGNIGGTYA 388

Query: 361 SPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKR 420
           SP++  P+VAI A+G+I+K+P+     +V P +IM V+  ADHRV+DGAT+A+F N WK 
Sbjct: 389 SPVVFPPQVAIGAIGKIEKLPRFDKHDNVIPVNIMKVSWCADHRVVDGATMARFSNRWKF 448

Query: 421 FIENPDLLILQMR 423
           ++E+P  ++ Q++
Sbjct: 449 YLEHPSAMLAQLK 448

[Close]

BLAST of Spg031615 vs. ExPASy Swiss-Prot
Match: P11181 (Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial OS=Bos taurus OX=9913 GN=DBT PE=1 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 2.0e-66
Identity = 139/363 (38.29%), Postives = 221/363 (60.88%), Query Frame = 0


Query: 69  VGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKH---KSGGVLSTPPIRNLAKQ 128
           VG+ L+ +  + L+D       ++E + +   V +    H   K    L+TP +R LA +
Sbjct: 131 VGKPLVDIETEALKD-------SEEDVVETPAVSHDEHTHQEIKGQKTLATPAVRRLAME 190

Query: 129 YGIDINDVRGTGVDGRVLKEDILQY------AVEKGILEEQSVCPAASPREQLGGSENYP 188
             I +++V G+G DGR+LKEDIL Y      A+     + + + P   P+++   +   P
Sbjct: 191 NNIKLSEVIGSGKDGRILKEDILNYLEKQTGAILPPSPKAEIMPPPPKPKDR---TIPIP 250

Query: 189 HTPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTT 248
            +   V +  +D+   ++GF +AMVK+M+ A K+PHF Y +E++   LV+L+   +    
Sbjct: 251 ISKPPV-FIGKDRTEPVKGFHKAMVKTMSAALKIPHFGYCDEVDLTELVKLREELKPIAF 310

Query: 249 EPDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNI 308
              +K +F+P  +K+ S+ + ++P+LN+  +E    +T K SHNIGIAM T  GL+VPN+
Sbjct: 311 ARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNV 370

Query: 309 KNVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVA 368
           KNVQ  S+ EI  EL+RLQ+L    +LS  D+ GGT TLSNIG+IGG +  P++  PEVA
Sbjct: 371 KNVQIRSIFEIATELNRLQKLGSAGQLSTNDLIGGTFTLSNIGSIGGTYAKPVILPPEVA 430

Query: 369 IIAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLIL 423
           I A+G IK +P+  + G+V  A IM V+  ADHR++DGATV++F N WK ++ENP  ++L
Sbjct: 431 IGALGTIKALPRFNEKGEVCKAQIMNVSWSADHRIIDGATVSRFSNLWKSYLENPAFMLL 482

[Close]

BLAST of Spg031615 vs. ExPASy TrEMBL
Match: A0A6J1C9A1 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Momordica charantia OX=3673 GN=LOC111009145 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 9.7e-186
Identity = 354/519 (68.21%), Postives = 377/519 (72.64%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MS++MIGRRKI P SAWAAGRRLL+P  SQG   +         P SIPEPSP LAC  F
Sbjct: 1   MSMIMIGRRKISPGSAWAAGRRLLSPFASQGSFPSLFQLPLEQTPKSIPEPSPPLACSRF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
           S  WL SPANRFE                                               
Sbjct: 61  STTWLQSPANRFEANRRGVSKICGFSSQALDGLPVFRLVDVPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV VDD ED 
Sbjct: 121 KEGDLVEAFQPLCEVQSDKATIEITSRYKGKVGQLLYVPGDIVKVGETLLKVGVDDFEDG 180

Query: 181 TQFSGLTKEQLAKPEL-VDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVL 240
           TQ SG T+  LA PEL +D Q+DK+K GGVLSTP +R+LAKQY IDINDVRGTG DGRVL
Sbjct: 181 TQVSGPTEGHLANPELTIDIQLDKNKCGGVLSTPAVRDLAKQYAIDINDVRGTGTDGRVL 240

Query: 241 KEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAM 300
           KEDILQYAVEKGILEEQ V  AASPREQ  GSE+  H PDEVRWSYEDKKVQLRGFQRAM
Sbjct: 241 KEDILQYAVEKGILEEQHVSSAASPREQFDGSESRTHAPDEVRWSYEDKKVQLRGFQRAM 300

Query: 301 VKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYP 360
           VKSMTIAAKVPHFHYVEEINCD L+ELKASFQGNT EPDVKHT LPLLIKSLSMAM KYP
Sbjct: 301 VKSMTIAAKVPHFHYVEEINCDGLLELKASFQGNTPEPDVKHTSLPLLIKSLSMAMNKYP 360

Query: 361 MLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMD 420
           MLNSCFNEES+EVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSV+EITKELSRLQQL++D
Sbjct: 361 MLNSCFNEESYEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVMEITKELSRLQQLALD 420

Query: 421 NKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASI 423
           NKL PGD+SGGTITLSNIGAIGGKFGSPLLNLPEVAIIA+GRIKK+PQI+DDGDVYPASI
Sbjct: 421 NKLGPGDISGGTITLSNIGAIGGKFGSPLLNLPEVAIIAIGRIKKIPQISDDGDVYPASI 480

[Close]

BLAST of Spg031615 vs. ExPASy TrEMBL
Match: A0A6J1I8C5 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita maxima OX=3661 GN=LOC111472314 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.8e-184
Identity = 352/518 (67.95%), Postives = 378/518 (72.97%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MSILMI RRKI P SAWAAGRRLL P+  QGP+A+         P S+PEP   LACPSF
Sbjct: 1   MSILMISRRKIFPTSAWAAGRRLLFPVAFQGPSASISQLPSEQTPKSLPEPYRTLACPSF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            R  LHSPANRFE                                               
Sbjct: 61  CRPGLHSPANRFEASIKGVSKICRFSSQALDGLPVFRTVDVPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV+VD+ ED 
Sbjct: 121 KEGEEVEEFQPICEVQSDKATIEITSRYKGKVSRLLYVPGDIVKVGETLLKVLVDEFEDG 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
           TQ SGLT+E LAKPELVD Q+DK K  GVLSTP +RNLAKQY IDINDVRGTG DGR+LK
Sbjct: 181 TQVSGLTEEHLAKPELVDSQLDKSKGVGVLSTPAVRNLAKQYDIDINDVRGTGTDGRILK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           +DILQYAV+KGI+EEQ V  A SP+EQL  SENYP+  DEV WSYEDKKVQLRG+QRAMV
Sbjct: 241 DDILQYAVKKGIIEEQRVSSAPSPKEQLVESENYPNAQDEVWWSYEDKKVQLRGYQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSMTI+AKVPHFHYVEEINCD LVELKA FQ NTT+PDVKHTFLPLLIKSLSMAM KYPM
Sbjct: 301 KSMTISAKVPHFHYVEEINCDGLVELKACFQENTTKPDVKHTFLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           +NSCFNEESFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSRLQ+L+MDN
Sbjct: 361 VNSCFNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSRLQRLAMDN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSP DVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQI +DGDVYPASIM
Sbjct: 421 KLSPVDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQITEDGDVYPASIM 480

[Close]

BLAST of Spg031615 vs. ExPASy TrEMBL
Match: A0A6J1G741 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucurbita moschata OX=3662 GN=LOC111451360 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 1.3e-182
Identity = 349/518 (67.37%), Postives = 377/518 (72.78%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAAT-----GRLKPNSIPEPSPQLACPSF 60
           MSILMI RRKI P S+WAAG RLL P+  QGP+A+         P S+PEP   LACPSF
Sbjct: 1   MSILMISRRKIFPTSSWAAGCRLLFPVAFQGPSASISQLPSVQTPKSLPEPYRTLACPSF 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            R  LHSPANRFE                                               
Sbjct: 61  CRPGLHSPANRFEASIKGVSKVCRFSSQALDGLPVFRTVDVLLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLK++VD+ E+ 
Sbjct: 121 KEGEEVEEFQPICEVQSDKATIEITSRYKGKVSRLLYVPGDIVKVGETLLKLLVDEFEEG 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
           T  SGLT+E LAKPELVD Q+DK K  GVLSTP +RNLAKQY IDINDVRGTG DGR+LK
Sbjct: 181 TPVSGLTEEHLAKPELVDSQLDKSKGIGVLSTPAVRNLAKQYDIDINDVRGTGTDGRILK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           +DILQYAV+KGI+EEQ V  A SP+EQL  SENYP+  DEVRWSYEDKKVQLRG+QRAMV
Sbjct: 241 DDILQYAVKKGIIEEQRVSSAPSPKEQLVESENYPNAQDEVRWSYEDKKVQLRGYQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSMTI+AKVPHFHYVEEINCD LVELKA FQ NTTEPDVKHTFLPLLIKSLSMAM KYPM
Sbjct: 301 KSMTISAKVPHFHYVEEINCDGLVELKACFQENTTEPDVKHTFLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           +NSCFNEESFEVTLKGSHNIGIAMATP+GLVVPNIK+VQSLSVLEITKELSRLQ+L+MDN
Sbjct: 361 VNSCFNEESFEVTLKGSHNIGIAMATPNGLVVPNIKSVQSLSVLEITKELSRLQRLAMDN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSP DVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQI +DGDVYPASIM
Sbjct: 421 KLSPVDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQITEDGDVYPASIM 480

[Close]

BLAST of Spg031615 vs. ExPASy TrEMBL
Match: A0A1S3AX39 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo OX=3656 GN=LOC103483861 PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 2.4e-176
Identity = 340/518 (65.64%), Postives = 372/518 (71.81%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAA-----TGRLKPNSIPEPSPQLACPSF 60
           MSIL IGRR+I P++AWAAG RLL+P TSQ P+A     + +  P S+P P P L   S 
Sbjct: 1   MSILTIGRRRISPRNAWAAGCRLLSPFTSQSPSASIPQFSSQQTPESLPHPFPPLGSLSS 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            RAWLHS ANRFE                                               
Sbjct: 61  GRAWLHSHANRFEAWIKGVSKICRFSSQALDGLPLFRLVDIPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV+V+  EDE
Sbjct: 121 QEGDEVEEFQPLCEVQSDKATIEITSRYKGKVGQLLYVPGDIVKVGETLLKVLVEGFEDE 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
            Q SGLT+  LAKPE+ + + DK K+ GVLSTPP+RNLAKQYGIDINDV G+G DGRVLK
Sbjct: 181 IQVSGLTEGHLAKPEVKESRQDKTKNCGVLSTPPVRNLAKQYGIDINDVSGSGTDGRVLK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           ED+LQYAVEKGILEE+ V  AAS REQ   SE + H PD V WSYEDKKVQLRGFQRAMV
Sbjct: 241 EDVLQYAVEKGILEER-VSSAASFREQFDESETHTHAPDGVTWSYEDKKVQLRGFQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSM IAAKVPHFHYVEEINCDAL+ELKASFQGNTTEP+VKHT LPLLIKSLSMAM KYPM
Sbjct: 301 KSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           LNSCFNE++FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQ L+M+N
Sbjct: 361 LNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMEN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSPGD+SGGTITLSNIGAIGGKFGSPLLNLPEV+IIA+GRIKKVPQI DDG VYP+SIM
Sbjct: 421 KLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDGGVYPSSIM 480

[Close]

BLAST of Spg031615 vs. ExPASy TrEMBL
Match: A0A5D3CXV6 (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001180 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.1e-176
Identity = 340/518 (65.64%), Postives = 371/518 (71.62%), Query Frame = 0


Query: 1   MSILMIGRRKICPKSAWAAGRRLLAPITSQGPAA-----TGRLKPNSIPEPSPQLACPSF 60
           MSIL IGRR+I P++AWAAG RLL+P TSQ P+A     + +  P S+P P P L   S 
Sbjct: 1   MSILTIGRRRISPRNAWAAGCRLLSPFTSQSPSASIPQFSSQQTPESLPHPFPPLGSLSS 60

Query: 61  SRAWLHSPANRFE----------------------------------------------- 120
            RAWLHS ANRFE                                               
Sbjct: 61  GRAWLHSHANRFEAWIKGVSKICRFSSQALDGLPLFRLVDIPLAQTGEGIAECELLKWFV 120

Query: 121 --------------------------------------------VGETLLKVIVDDLEDE 180
                                                       VGETLLKV+V+  EDE
Sbjct: 121 QEGDEVEEFQPLCEVQSDKATIEITSRYKGKVGQLLYVPGDIVKVGETLLKVLVEGFEDE 180

Query: 181 TQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIRNLAKQYGIDINDVRGTGVDGRVLK 240
            Q SGLT+  LAKPE+ + + DK K+ GVLSTPP+RNLAKQYGIDINDV G+G DGRVLK
Sbjct: 181 IQVSGLTEGHLAKPEVKESRQDKTKNCGVLSTPPVRNLAKQYGIDINDVSGSGTDGRVLK 240

Query: 241 EDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVRWSYEDKKVQLRGFQRAMV 300
           ED+LQYAVEKGILEE  V  AAS REQ   SE + H PD V WSYEDKKVQLRGFQRAMV
Sbjct: 241 EDVLQYAVEKGILEEH-VSSAASFREQFDESETHTHAPDGVTWSYEDKKVQLRGFQRAMV 300

Query: 301 KSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPM 360
           KSM IAAKVPHFHYVEEINCDAL+ELKASFQGNTTEP+VKHT LPLLIKSLSMAM KYPM
Sbjct: 301 KSMAIAAKVPHFHYVEEINCDALLELKASFQGNTTEPNVKHTSLPLLIKSLSMAMSKYPM 360

Query: 361 LNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQQLSMDN 420
           LNSCFNE++FEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQ L+M+N
Sbjct: 361 LNSCFNEDAFEVTLKGSHNIGIAMATPHGLVVPNIKNVQSLSVLEITKELSRLQLLAMEN 420

Query: 421 KLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAIIAMGRIKKVPQIADDGDVYPASIM 423
           KLSPGD+SGGTITLSNIGAIGGKFGSPLLNLPEV+IIA+GRIKKVPQI DDG VYP+SIM
Sbjct: 421 KLSPGDISGGTITLSNIGAIGGKFGSPLLNLPEVSIIAIGRIKKVPQITDDGGVYPSSIM 480

[Close]

BLAST of Spg031615 vs. TAIR 10
Match: AT3G06850.1 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 436.0 bits (1120), Expect = 3.4e-122
Identity = 224/362 (61.88%), Postives = 274/362 (75.69%), Query Frame = 0


Query: 61  HSPANRFEVGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIR 120
           HSP +  +VGETL+++ V+D +D    S LT +      L   +       G LSTP +R
Sbjct: 134 HSPGDIIKVGETLVRLAVEDSQD----SLLTTDSSEIVTLGGSKQGTENLLGALSTPAVR 193

Query: 121 NLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPH 180
           NLAK  GIDIN + GTG DGRVLKED+L+++ +KG + +      +S    +GG      
Sbjct: 194 NLAKDLGIDINVITGTGKDGRVLKEDVLRFSDQKGFVTDS----VSSEHAVIGGDS---- 253

Query: 181 TPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTE 240
              +   ++EDK V LRGF RAMVK+MT+A  VPHFH+VEEINCD+LVELK  F+ N T+
Sbjct: 254 VSTKASSNFEDKTVPLRGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTD 313

Query: 241 PDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIK 300
             +KHTFLP LIKSLSMA+ KYP +NSCFN ES E+ LKGSHNIG+AMAT HGLVVPNIK
Sbjct: 314 STIKHTFLPTLIKSLSMALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIK 373

Query: 301 NVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAI 360
           NVQSLS+LEITKELSRLQ L+ +NKL+P DV+GGTITLSNIGAIGGKFGSPLLNLPEVAI
Sbjct: 374 NVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAI 433

Query: 361 IAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQ 420
           IA+GRI+KVP+ + +G VYPASIM VNI ADHRVLDGATVA+FC +WK ++E P+LL+LQ
Sbjct: 434 IALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQ 483

Query: 421 MR 423
           MR
Sbjct: 494 MR 483

[Close]

BLAST of Spg031615 vs. TAIR 10
Match: AT3G06850.2 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 436.0 bits (1120), Expect = 3.4e-122
Identity = 224/362 (61.88%), Postives = 274/362 (75.69%), Query Frame = 0


Query: 61  HSPANRFEVGETLLKVIVDDLEDETQFSGLTKEQLAKPELVDYQVDKHKSGGVLSTPPIR 120
           HSP +  +VGETL+++ V+D +D    S LT +      L   +       G LSTP +R
Sbjct: 134 HSPGDIIKVGETLVRLAVEDSQD----SLLTTDSSEIVTLGGSKQGTENLLGALSTPAVR 193

Query: 121 NLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPH 180
           NLAK  GIDIN + GTG DGRVLKED+L+++ +KG + +      +S    +GG      
Sbjct: 194 NLAKDLGIDINVITGTGKDGRVLKEDVLRFSDQKGFVTDS----VSSEHAVIGGDS---- 253

Query: 181 TPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKASFQGNTTE 240
              +   ++EDK V LRGF RAMVK+MT+A  VPHFH+VEEINCD+LVELK  F+ N T+
Sbjct: 254 VSTKASSNFEDKTVPLRGFSRAMVKTMTMATSVPHFHFVEEINCDSLVELKQFFKENNTD 313

Query: 241 PDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIK 300
             +KHTFLP LIKSLSMA+ KYP +NSCFN ES E+ LKGSHNIG+AMAT HGLVVPNIK
Sbjct: 314 STIKHTFLPTLIKSLSMALTKYPFVNSCFNAESLEIILKGSHNIGVAMATEHGLVVPNIK 373

Query: 301 NVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAI 360
           NVQSLS+LEITKELSRLQ L+ +NKL+P DV+GGTITLSNIGAIGGKFGSPLLNLPEVAI
Sbjct: 374 NVQSLSLLEITKELSRLQHLAANNKLNPEDVTGGTITLSNIGAIGGKFGSPLLNLPEVAI 433

Query: 361 IAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFIENPDLLILQ 420
           IA+GRI+KVP+ + +G VYPASIM VNI ADHRVLDGATVA+FC +WK ++E P+LL+LQ
Sbjct: 434 IALGRIEKVPKFSKEGTVYPASIMMVNIAADHRVLDGATVARFCCQWKEYVEKPELLMLQ 483

Query: 421 MR 423
           MR
Sbjct: 494 MR 483

[Close]

BLAST of Spg031615 vs. TAIR 10
Match: AT3G25860.1 (2-oxoacid dehydrogenases acyltransferase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 7.5e-29
Identity = 93/308 (30.19%), Postives = 147/308 (47.73%), Query Frame = 0


Query: 114 LSTPPIRNLAKQYGIDINDVRGTGVDGRVLKEDILQYAVEKGIL-EEQSVCPAASPREQL 173
           ++TP  + LAKQ+ +DI  V GTG  GR+   D+   A   GI   + S+ P   P   +
Sbjct: 187 VATPYAKKLAKQHKVDIESVAGTGPFGRITASDVETAA---GIAPSKSSIAPPPPPPPPV 246

Query: 174 GGSENYPHTPDEVRWSYEDKKVQLRGFQRAMVKSMTIAAKVPHFHYVEEINCDALVELKA 233
                  + P  +    +   V     Q A+ K+M  +  VP F     +N DAL  L  
Sbjct: 247 TAKATTTNLPPLLP---DSSIVPFTAMQSAVSKNMIESLSVPTFRVGYPVNTDALDALYE 306

Query: 234 SFQGNTTEPDVKHTFLPLLIKSLSMAMGKYPMLN-SCFNEESFEVTLKGSHNIGIAMATP 293
             +          T   LL K+  MA+ ++P++N SC + +SF  +   S NI +A+A  
Sbjct: 307 KVKPKGV------TMTALLAKAAGMALAQHPVVNASCKDGKSF--SYNSSINIAVAVAIN 366

Query: 294 HGLVVPNIKNVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSP 353
            GL+ P +++   L +  ++++   L   +   +L P + + GT TLSN+G  G      
Sbjct: 367 GGLITPVLQDADKLDLYLLSQKWKELVGKARSKQLQPHEYNSGTFTLSNLGMFGVDRFDA 426

Query: 354 LLNLPEVAIIAMGRIKKVPQIADDGDVYPASIMTVNIGADHRVLDGATVAKFCNEWKRFI 413
           +L   + AI+A+G  K       DG     + M VN+ ADHR++ GA +A F   + + I
Sbjct: 427 ILPPGQGAIMAVGASKPTVVADKDGFFSVKNTMLVNVTADHRIVYGADLAAFLQTFAKII 480

Query: 414 ENPDLLIL 420
           ENPD L L
Sbjct: 487 ENPDSLTL 480

[Close]

BLAST of Spg031615 vs. TAIR 10
Match: AT3G52200.1 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 96/363 (26.45%), Postives = 174/363 (47.93%), Query Frame = 0


Query: 74  LKVIVDDLED----ETQFSGLTKEQLAKPELVDYQVDK---HKSGGVLSTPPIRNLAKQY 133
           + +IV+D E     ++  +G ++    K E+ D  VDK    K+G    +P  + L  ++
Sbjct: 284 IALIVEDAESIEAIKSSSAGSSEVDTVK-EVPDSVVDKPTERKAGFTKISPAAKLLILEH 343

Query: 134 GIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVR 193
           G++ + +  +G  G +LK D++  A+  G   + S    AS +++    E    +    +
Sbjct: 344 GLEASSIEASGPYGTLLKSDVVA-AIASGKASKSS----ASTKKKQPSKETPSKSSSTSK 403

Query: 194 WSYEDKKVQLRGFQRAMVKS------MTIAAKVPHFHYVEEINCDALVELKASFQGNTTE 253
            S          F  + ++       +    K+PH +   ++  D L+  +   Q N   
Sbjct: 404 PSVTQSDNNYEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDVVLDPLLAFRKELQEN--- 463

Query: 254 PDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIK 313
             VK +   ++IK++++A+      N+ ++ E  ++ +  S +I IA+AT  GL+ P IK
Sbjct: 464 HGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDSVDISIAVATEKGLMTPIIK 523

Query: 314 NVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAI 373
           N    S+  I+ E+  L Q +   KL+P +  GGT ++SN+G         ++N P+  I
Sbjct: 524 NADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNLGMYPVDNFCAIINPPQAGI 583

Query: 374 IAMGRIKKV--PQIADDGDVYPASI--MTVNIGADHRVLDGATVAKFCNEWKRFIENPDL 420
           +A+GR  KV  P I  DG   P+ +  M V + ADHR+ DG   A F +E +   E+   
Sbjct: 584 LAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDGQVGASFMSELRSNFEDVRR 637

[Close]

BLAST of Spg031615 vs. TAIR 10
Match: AT3G52200.2 (Dihydrolipoamide acetyltransferase, long form protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 96/363 (26.45%), Postives = 174/363 (47.93%), Query Frame = 0


Query: 74  LKVIVDDLED----ETQFSGLTKEQLAKPELVDYQVDK---HKSGGVLSTPPIRNLAKQY 133
           + +IV+D E     ++  +G ++    K E+ D  VDK    K+G    +P  + L  ++
Sbjct: 360 IALIVEDAESIEAIKSSSAGSSEVDTVK-EVPDSVVDKPTERKAGFTKISPAAKLLILEH 419

Query: 134 GIDINDVRGTGVDGRVLKEDILQYAVEKGILEEQSVCPAASPREQLGGSENYPHTPDEVR 193
           G++ + +  +G  G +LK D++  A+  G   + S    AS +++    E    +    +
Sbjct: 420 GLEASSIEASGPYGTLLKSDVVA-AIASGKASKSS----ASTKKKQPSKETPSKSSSTSK 479

Query: 194 WSYEDKKVQLRGFQRAMVKS------MTIAAKVPHFHYVEEINCDALVELKASFQGNTTE 253
            S          F  + ++       +    K+PH +   ++  D L+  +   Q N   
Sbjct: 480 PSVTQSDNNYEDFPNSQIRKIIAKRLLESKQKIPHLYLQSDVVLDPLLAFRKELQEN--- 539

Query: 254 PDVKHTFLPLLIKSLSMAMGKYPMLNSCFNEESFEVTLKGSHNIGIAMATPHGLVVPNIK 313
             VK +   ++IK++++A+      N+ ++ E  ++ +  S +I IA+AT  GL+ P IK
Sbjct: 540 HGVKVSVNDIVIKAVAVALRNVRQANAFWDAEKGDIVMCDSVDISIAVATEKGLMTPIIK 599

Query: 314 NVQSLSVLEITKELSRLQQLSMDNKLSPGDVSGGTITLSNIGAIGGKFGSPLLNLPEVAI 373
           N    S+  I+ E+  L Q +   KL+P +  GGT ++SN+G         ++N P+  I
Sbjct: 600 NADQKSISAISLEVKELAQKARSGKLAPHEFQGGTFSISNLGMYPVDNFCAIINPPQAGI 659

Query: 374 IAMGRIKKV--PQIADDGDVYPASI--MTVNIGADHRVLDGATVAKFCNEWKRFIENPDL 420
           +A+GR  KV  P I  DG   P+ +  M V + ADHR+ DG   A F +E +   E+   
Sbjct: 660 LAVGRGNKVVEPVIGLDGIEKPSVVTKMNVTLSADHRIFDGQVGASFMSELRSNFEDVRR 713

The following BLAST results are available for this feature:

BLAST of Spg031615 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137797.1	2.0e-185	68.21	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_022973737.1	3.8e-184	67.95	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_038883446.1	7.2e-183	72.92	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
XP_022947515.1	2.7e-182	67.37	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
KAG6597534.1	7.4e-180	66.93	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]

BLAST of Spg031615 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M7Z1	4.7e-121	61.88	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P11182	5.5e-69	39.57	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P53395	3.6e-68	39.72	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
Q23571	3.0e-67	39.41	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]
P11181	2.0e-66	38.29	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogen...	[more]

BLAST of Spg031615 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9A1	9.7e-186	68.21	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A6J1I8C5	1.8e-184	67.95	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A6J1G741	1.3e-182	67.37	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A1S3AX39	2.4e-176	65.64	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]
A0A5D3CXV6	3.1e-176	65.64	Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex O...	[more]

BLAST of Spg031615 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06850.1	3.4e-122	61.88	2-oxoacid dehydrogenases acyltransferase family protein	[more]
AT3G06850.2	3.4e-122	61.88	2-oxoacid dehydrogenases acyltransferase family protein	[more]
AT3G25860.1	7.5e-29	30.19	2-oxoacid dehydrogenases acyltransferase family protein	[more]
AT3G52200.1	1.7e-28	26.45	Dihydrolipoamide acetyltransferase, long form protein	[more]
AT3G52200.2	1.7e-28	26.45	Dihydrolipoamide acetyltransferase, long form protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036625	E3-binding domain superfamily	GENE3D	4.10.320.10		coord: 107..155 e-value: 6.3E-14 score: 53.3
IPR036625	E3-binding domain superfamily	SUPERFAMILY	47005	Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex	coord: 108..150
IPR004167	Peripheral subunit-binding domain	PFAM	PF02817	E3_binding	coord: 113..148 e-value: 9.6E-13 score: 48.2
IPR004167	Peripheral subunit-binding domain	PROSITE	PS51826	PSBD	coord: 114..151 score: 19.691856
IPR001078	2-oxoacid dehydrogenase acyltransferase, catalytic domain	PFAM	PF00198	2-oxoacid_dh	coord: 190..418 e-value: 1.7E-75 score: 253.5
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 179..419 e-value: 6.3E-80 score: 270.2
None	No IPR available	PANTHER	PTHR43178	DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX	coord: 61..421
None	No IPR available	PANTHER	PTHR43178:SF5	LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL	coord: 61..421
None	No IPR available	SUPERFAMILY	52777	CoA-dependent acyltransferases	coord: 186..419

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031615.1	Spg031615.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0016407	acetyltransferase activity
molecular_function	GO:0031405	lipoic acid binding
molecular_function	GO:0016746	acyltransferase activity