Spg031489 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031489 vs. NCBI nr
Match: XP_023539324.1 (endo-1,3;1,4-beta-D-glucanase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437.6 bits (1124), Expect = 7.0e-119
Identity = 212/239 (88.70%), Postives = 227/239 (94.98%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNPS GAGHVEQLGGLS+YV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPSSGAGHVEQLGGLSTYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDP+VAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPYVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA +SVLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVSVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Spg031489 vs. NCBI nr
Match: KAG7028931.1 (hypothetical protein SDJN02_10114 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 435.6 bits (1119), Expect = 2.7e-118
Identity = 212/239 (88.70%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNP+ GAGHVEQLGGLS+YV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPTSGAGHVEQLGGLSTYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPFVAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA ISVLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHISVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVD FVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDAFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Spg031489 vs. NCBI nr
Match: XP_022973692.1 (endo-1,3;1,4-beta-D-glucanase [Cucurbita maxima] >XP_022973693.1 endo-1,3;1,4-beta-D-glucanase [Cucurbita maxima])

HSP 1 Score: 435.6 bits (1119), Expect = 2.7e-118
Identity = 212/239 (88.70%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNPS GAGHVEQLGGLSSYV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPSSGAGHVEQLGGLSSYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPFVAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA ++VLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVAVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAH DLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHRDLLAWFTKYLK 239

BLAST of Spg031489 vs. NCBI nr
Match: XP_022936922.1 (endo-1,3;1,4-beta-D-glucanase [Cucurbita moschata])

HSP 1 Score: 435.3 bits (1118), Expect = 3.5e-118
Identity = 211/239 (88.28%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNP+ GAGHVEQLGGLS+YV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPTSGAGHVEQLGGLSAYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPFVAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA +SVLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVSVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVD FVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDAFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Spg031489 vs. NCBI nr
Match: XP_008438729.1 (PREDICTED: endo-1,3;1,4-beta-D-glucanase-like isoform X1 [Cucumis melo] >KAA0049403.1 endo-1,3 [Cucumis melo var. makuwa] >TYK16081.1 endo-1,3 [Cucumis melo var. makuwa])

HSP 1 Score: 429.1 bits (1102), Expect = 2.5e-116
Identity = 204/239 (85.36%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPP+LNPS GAGH+EQLGGL++YV+GSPDSK+AV+ ITDVYG+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDPFV +DANRPI+ WL+DHGP+K FDD K +VEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVV LA  ELIQAAVLLHPSFVTVDDIKGVKA IS+LGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE+LSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAH DLLAWFT+Y+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Spg031489 vs. ExPASy Swiss-Prot
Match: Q9ZT66 (Endo-1,3;1,4-beta-D-glucanase OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.5e-34
Identity = 89/200 (44.50%), Postives = 119/200 (59.50%), Query Frame = 0

Query: 2   AGPQCCSNPPSLN----PSCGAGHV--EQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPL 61
           A  QC  NPP  +        AG V  +  GGL +YV+G+  S  AV++ +DV+GYEAPL
Sbjct: 25  AHSQCLDNPPDRSIHGRQLAEAGEVVHDLPGGLRAYVSGAASSSRAVVLASDVFGYEAPL 84

Query: 62  LRKLADKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKS 121
           LR++ DKVA AG+FVV PDF KGD     D  +    WL+ H P KA +D K +  ALK 
Sbjct: 85  LRQIVDKVAKAGYFVVVPDFLKGDYL---DDKKNFTEWLEAHSPVKAAEDAKPLFAALKK 144

Query: 122 KGIS-AIGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQ 181
           +G S A+G  G CWG K+ V +     ++A  L HP  VT DD+K VK PI +LGA+ D 
Sbjct: 145 EGKSVAVG--GYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDT 204

Query: 182 LSPPELLKQFEEVLSAKPEV 195
            +PP+ + +F  VL  + EV
Sbjct: 205 TTPPKEVYRFVHVLRERHEV 219

BLAST of Spg031489 vs. ExPASy Swiss-Prot
Match: A0A2V5GSC6 (Hydrolase pyvD OS=Aspergillus violaceofuscus (strain CBS 115571) OX=1450538 GN=pyvD PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.7e-25
Identity = 71/239 (29.71%), Postives = 120/239 (50.21%), Query Frame = 0

Query: 5   QCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKL--AVIVITDVYGYEAPLLRKLADK 64
           QCC    SL+     G + + G +  YV+  PD     A++ ++D++G      + +AD 
Sbjct: 8   QCCIG-GSLHEGETQGELTKFGDIPVYVSYPPDKSTHNAILFLSDIFGLALVNSKLIADL 67

Query: 65  VAAAGFFVVAPDFFKGDPF-VANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSK-GISA 124
            AA G+ VV PD F+GDP  V +  +  I  WL+ H P +      A +  ++ + G   
Sbjct: 68  FAANGYLVVMPDLFQGDPVPVDHSPSFQIMDWLQGHLPPQTDPIVTATLREMRERLGCQR 127

Query: 125 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 184
           IG +G C+G K VV       +    + HP+F+  D++K ++ P+S+  +  D L PPE 
Sbjct: 128 IGGVGYCYGGKYVVRYLHPGQLDVGFVAHPTFIEPDELKAIEGPLSISASAQDNLFPPEK 187

Query: 185 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
             + E +L A+ +V   + IF  V HG+ VR ++         +++    + WF +YLK
Sbjct: 188 RHESETIL-AQLDVPYQINIFSDVEHGFAVRCDLNKTRHRFAKEQSFSQGVTWFNQYLK 244

BLAST of Spg031489 vs. ExPASy Swiss-Prot
Match: Q8R1G2 (Carboxymethylenebutenolidase homolog OS=Mus musculus OX=10090 GN=Cmbl PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 2.7e-20
Identity = 71/226 (31.42%), Postives = 112/226 (49.56%), Query Frame = 0

Query: 18  GAGHVEQLGGLSSYVTGSP-DSKLAVIVITDVYGYEAPLLRKLADKVAAAGFFVVAPDFF 77
           G GH  Q+  + +YVT SP D+  AVIV+ D++G++ P  R +AD +A  G+  + PDFF
Sbjct: 20  GMGHEVQVEHIKAYVTRSPVDAGKAVIVVQDIFGWQLPNTRYMADMIARNGYTTIVPDFF 79

Query: 78  KG-DPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSK-GISAIGAIGICWGAKVVV 137
            G +P+          AWLK     K   +  A++  L+ +     IG +G CWG  VV 
Sbjct: 80  VGQEPWDPAGDWSTFPAWLKSRNARKVNREVDAVLRYLRQQCHAQKIGIVGFCWGGVVVH 139

Query: 138 ALATA-ELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPELLKQFEEVLSAKPE 197
            + TA   I+A V ++      +D+  +K P   + AE D + P E +    + L     
Sbjct: 140 QVMTAYPDIRAGVSVYGIIRDSEDVYNLKNPTLFIFAENDTVIPLEQVSTLTQKLKEHCI 199

Query: 198 VDGFVKIFPKVSHGWTVRYNVEDEEAVK-CADEAHGDLLAWFTRYL 239
           V+  VK F   +HG+  R   +   A K   +EA  +L+ W  +Y+
Sbjct: 200 VNYQVKTFSGQTHGFVHRKREDCSPADKPYIEEARRNLIEWLNKYV 245

BLAST of Spg031489 vs. ExPASy Swiss-Prot
Match: Q6P7K0 (Carboxymethylenebutenolidase homolog OS=Xenopus tropicalis OX=8364 GN=cmbl PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.5e-20
Identity = 69/225 (30.67%), Postives = 113/225 (50.22%), Query Frame = 0

Query: 20  GHVEQLGGLSSYVTGSPDS-KLAVIVITDVYGYEAPLLRKLADKVAAAGFFVVAPDFFKG 79
           G   Q+  + +YV+    S   AVIV+ D++G++ P  R +AD + A G+  + PDFF G
Sbjct: 22  GQEVQIEHIKAYVSKPHSSTDKAVIVVQDIFGWQLPNTRFMADLLTAHGYITICPDFFVG 81

Query: 80  -DPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSK-GISAIGAIGICWGAKVV--V 139
            +P+  ++       WL+     K   +   +++ LK +  +  IG IG CWG  V   +
Sbjct: 82  QEPWKPSNDRSTFTEWLQTRQATKVEKEINVVLKYLKEQCHVKKIGVIGFCWGGVVTHHL 141

Query: 140 ALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPELLKQFEEVLSAKPEV 199
            L   EL +A V  +     V+D   +  P   + AE+D + P E +   EE L    +V
Sbjct: 142 MLKYPEL-KAGVSFYGIIRDVEDRYNLLNPTLFIFAEMDHVIPLEQVSLLEEKLKVHSKV 201

Query: 200 DGFVKIFPKVSHGWTVRYNVE-DEEAVKCADEAHGDLLAWFTRYL 239
           D  VK+FPK +HG+  R N + + E     +EA  ++L W  +Y+
Sbjct: 202 DFQVKVFPKQTHGFVHRKNEDINPEDKPFIEEARKNMLEWLHKYI 245

BLAST of Spg031489 vs. ExPASy Swiss-Prot
Match: Q5XH09 (Carboxymethylenebutenolidase homolog OS=Xenopus laevis OX=8355 GN=cmbl PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 5.0e-19
Identity = 69/228 (30.26%), Postives = 113/228 (49.56%), Query Frame = 0

Query: 20  GHVEQLGGLSSYVTGSPDS-KLAVIVITDVYGYEAPLLRKLADKVAAAGFFVVAPDFFKG 79
           G   Q+  + +YV+    S   AVIV+ D++G++ P  R +AD + A G+  + PDFF G
Sbjct: 22  GQEIQIEHIKAYVSKPHSSTDKAVIVVQDIFGWQLPNTRFMADLLTAHGYITICPDFFVG 81

Query: 80  -DPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSK-GISAIGAIGICWGAKVV--V 139
            + +  ++       WL+     K   +   +++ LK +  +  IG IG CWG  V   +
Sbjct: 82  QESWKPSNDWSTFTEWLQTRQATKVEKEMNVVLKYLKEQCHVKKIGVIGFCWGGVVTHHL 141

Query: 140 ALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPELLKQFEEVLSAKPEV 199
            L   EL +A V  +     V+D   +  P   + AEID + P E +   E+ L    +V
Sbjct: 142 MLKYPEL-KAGVSFYGIIRDVEDRYNLLNPTLFIFAEIDHVIPLEQVSLLEQKLKVHSKV 201

Query: 200 DGFVKIFPKVSHGWTVR----YNVEDEEAVKCADEAHGDLLAWFTRYL 239
           D  +K+FPK +HG+  R     N ED+  +   +EA  D+L W  +Y+
Sbjct: 202 DFQIKVFPKQTHGFVHRKKEDINPEDKPFI---EEARKDMLEWLQKYI 245

BLAST of Spg031489 vs. ExPASy TrEMBL
Match: A0A6J1IDX2 (endo-1,31,4-beta-D-glucanase OS=Cucurbita maxima OX=3661 GN=LOC111472278 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.3e-118
Identity = 212/239 (88.70%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNPS GAGHVEQLGGLSSYV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPSSGAGHVEQLGGLSSYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPFVAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA ++VLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVAVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAH DLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHRDLLAWFTKYLK 239

BLAST of Spg031489 vs. ExPASy TrEMBL
Match: A0A6J1F9P2 (endo-1,31,4-beta-D-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111443361 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.7e-118
Identity = 211/239 (88.28%), Postives = 226/239 (94.56%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPPSLNP+ GAGHVEQLGGLS+YV+G+PDSK AVI ITDVYGYEAPLLRKLA
Sbjct: 1   MSGPQCCSNPPSLNPTSGAGHVEQLGGLSAYVSGAPDSKNAVIFITDVYGYEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFFKGDPFVAND NRPI+ WL++HGPEK+FDDGKA+VEALKSKGISA
Sbjct: 61  DKVAAAGFFVVAPDFFKGDPFVANDPNRPIKEWLQEHGPEKSFDDGKAVVEALKSKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVVALA  ELIQAAVLLHPS VTVDDIK VKA +SVLGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVALAKVELIQAAVLLHPSLVTVDDIKEVKAHVSVLGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE L+AKPEVD FVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFT+YLK
Sbjct: 181 LKQFEEALAAKPEVDAFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTKYLK 239

BLAST of Spg031489 vs. ExPASy TrEMBL
Match: A0A5D3CVZ9 (Endo-1,3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00010 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.2e-116
Identity = 204/239 (85.36%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPP+LNPS GAGH+EQLGGL++YV+GSPDSK+AV+ ITDVYG+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDPFV +DANRPI+ WL+DHGP+K FDD K +VEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVV LA  ELIQAAVLLHPSFVTVDDIKGVKA IS+LGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE+LSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAH DLLAWFT+Y+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Spg031489 vs. ExPASy TrEMBL
Match: A0A1S3AWR8 (endo-1,31,4-beta-D-glucanase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483753 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.2e-116
Identity = 204/239 (85.36%), Postives = 225/239 (94.14%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPP+LNPS GAGH+EQLGGL++YV+GSPDSK+AV+ ITDVYG+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKIAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDPFV +DANRPI+ WL+DHGP+K FDD K +VEALK+KGISA
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHGPDKGFDDAKPVVEALKNKGISA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVV LA  ELIQAAVLLHPSFVTVDDIKGVKA IS+LGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAHISILGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE+LSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAH DLLAWFT+Y+K
Sbjct: 181 LKQFEEILSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Spg031489 vs. ExPASy TrEMBL
Match: A0A0A0LAG0 (DLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150110 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.1e-115
Identity = 202/239 (84.52%), Postives = 223/239 (93.31%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCCSNPP+LNPS GAGH+EQLGGL++YV+GSPDSKLAV+ ITDVYG+EAPLLRKLA
Sbjct: 1   MSGPQCCSNPPTLNPSSGAGHIEQLGGLTTYVSGSPDSKLAVLFITDVYGFEAPLLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAAAGFFVVAPDFF GDPFV +DANRPI+ WL+DH  EK FDD K +VEALK+KGI+A
Sbjct: 61  DKVAAAGFFVVAPDFFHGDPFVPDDANRPIRVWLQDHETEKGFDDAKPVVEALKNKGITA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA+GICWGAKVVV LA  ELIQAAVLLHPSFVTVDDIKGVKAPIS+LGAEID +SPPEL
Sbjct: 121 IGAVGICWGAKVVVELAKVELIQAAVLLHPSFVTVDDIKGVKAPISILGAEIDHMSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LK+FEE+LSAKPEVDGFVKIFPKVSHGWTVRY VEDEEAVKCADEAH DLLAWFT+Y+K
Sbjct: 181 LKEFEEILSAKPEVDGFVKIFPKVSHGWTVRYKVEDEEAVKCADEAHEDLLAWFTKYVK 239

BLAST of Spg031489 vs. TAIR 10
Match: AT3G23600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 6.8e-96
Identity = 159/239 (66.53%), Postives = 204/239 (85.36%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCC NPP+LNP  G+GHVE+LGGL +YV+GS +SKL V++I+D++G+EAP LR LA
Sbjct: 1   MSGPQCCENPPTLNPVSGSGHVEKLGGLDAYVSGSAESKLCVLLISDIFGFEAPNLRALA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAA+GF+VV PD+F GDP+  ++ +RPI  W+KDHG +K F++ K ++E +K+KGI+A
Sbjct: 61  DKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGITA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA G+CWGAKVVV L+  ELIQAAVLLHPSFV VDDIKG KAPI++LGAEIDQ+SPP L
Sbjct: 121 IGAAGMCWGAKVVVELSKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPAL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE+LS+KPEV+ +VKI PKVSHGWTVRYN+++ EAVK A+EAH ++L WF  Y+K
Sbjct: 181 LKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 239

BLAST of Spg031489 vs. TAIR 10
Match: AT3G23600.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 337.8 bits (865), Expect = 7.1e-93
Identity = 156/239 (65.27%), Postives = 201/239 (84.10%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+GPQCC NPP+LNP  G+GHVE+LGGL +YV+GS +SKL V++I+D++G+EAP LR LA
Sbjct: 1   MSGPQCCENPPTLNPVSGSGHVEKLGGLDAYVSGSAESKLCVLLISDIFGFEAPNLRALA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVAA+GF+VV PD+F GDP+  ++ +RPI  W+KDHG +K F++ K ++E +K+KGI+A
Sbjct: 61  DKVAASGFYVVVPDYFGGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIKNKGITA 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA G+CWG   VV L+  ELIQAAVLLHPSFV VDDIKG KAPI++LGAEIDQ+SPP L
Sbjct: 121 IGAAGMCWG---VVELSKEELIQAAVLLHPSFVNVDDIKGGKAPIAILGAEIDQMSPPAL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           LKQFEE+LS+KPEV+ +VKI PKVSHGWTVRYN+++ EAVK A+EAH ++L WF  Y+K
Sbjct: 181 LKQFEEILSSKPEVNSYVKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWFVTYIK 236

BLAST of Spg031489 vs. TAIR 10
Match: AT3G23570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 2.7e-84
Identity = 147/239 (61.51%), Postives = 182/239 (76.15%), Query Frame = 0

Query: 1   MAGPQCCSNPPSLNPSCGAGHVEQLGGLSSYVTGSPDSKLAVIVITDVYGYEAPLLRKLA 60
           M+G QC  NPP L+P+ G+GHVE+LG L +YV GS  SKLAV+++  V+GYE P LRKLA
Sbjct: 1   MSGHQCTENPPDLDPTSGSGHVEKLGNLDTYVCGSTHSKLAVLLVPHVFGYETPNLRKLA 60

Query: 61  DKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEKAFDDGKAIVEALKSKGISA 120
           DKVA AGF+ V PDFF GDP+   + +RP   W+KDH  EK F++ K IVEALK+KGI++
Sbjct: 61  DKVAEAGFYAVVPDFFHGDPYNPENQDRPFPIWMKDHELEKGFEESKPIVEALKNKGITS 120

Query: 121 IGAIGICWGAKVVVALATAELIQAAVLLHPSFVTVDDIKGVKAPISVLGAEIDQLSPPEL 180
           IGA G CWGAKV V LA  +L+ A VLLHP+ VTVDDIK V  PI+VLGAEIDQ+SPPEL
Sbjct: 121 IGAAGFCWGAKVAVELAKEKLVDATVLLHPARVTVDDIKEVNLPIAVLGAEIDQVSPPEL 180

Query: 181 LKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYNVEDEEAVKCADEAHGDLLAWFTRYLK 240
           ++QFE++L++KP+V  FVKIFP+  HGWTVRYN  D   V+ A EAH D+LAW   YLK
Sbjct: 181 VRQFEDILASKPQVKSFVKIFPRCKHGWTVRYNENDPSEVEAAMEAHKDMLAWLIDYLK 239

BLAST of Spg031489 vs. TAIR 10
Match: AT1G35420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.1e-12
Identity = 54/205 (26.34%), Postives = 96/205 (46.83%), Query Frame = 0

Query: 42  VIVITDVYGYEAPLLRKLADKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEK 101
           +++++DV+G++    R  A +VA  G+ V+ PD F+GDP+  N      + W + H P +
Sbjct: 111 LLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNR 170

Query: 102 AFDD----GKAIVEALKSKGIS-AIGAIGICW-GAKVVVALATAE--LIQAAVLLHPSFV 161
              D     K +VE   + GIS  +G +G C+ G +VV  LAT E       V  + + +
Sbjct: 171 IRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRI 230

Query: 162 TVDDIKGVKAPISVLGAEIDQLSPPELLKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYN 221
                  VK P+  +  + D L   + L + EE +    +    V ++    HG+  R  
Sbjct: 231 DSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSK----VVVYEGRGHGFVHRPE 290

Query: 222 VEDEEAVKCADEAHGDLLAWFTRYL 239
             +++  + A+EA   +  W   +L
Sbjct: 291 TPEDD--RDAEEAFALMRNWLHHHL 309

BLAST of Spg031489 vs. TAIR 10
Match: AT1G35420.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.1e-12
Identity = 54/205 (26.34%), Postives = 96/205 (46.83%), Query Frame = 0

Query: 42  VIVITDVYGYEAPLLRKLADKVAAAGFFVVAPDFFKGDPFVANDANRPIQAWLKDHGPEK 101
           +++++DV+G++    R  A +VA  G+ V+ PD F+GDP+  N      + W + H P +
Sbjct: 116 LLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFRGDPWSKNRPKSEYEEWRRGHDPNR 175

Query: 102 AFDD----GKAIVEALKSKGIS-AIGAIGICW-GAKVVVALATAE--LIQAAVLLHPSFV 161
              D     K +VE   + GIS  +G +G C+ G +VV  LAT E       V  + + +
Sbjct: 176 IRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGRVVDVLATDESGYFSTGVSFYGTRI 235

Query: 162 TVDDIKGVKAPISVLGAEIDQLSPPELLKQFEEVLSAKPEVDGFVKIFPKVSHGWTVRYN 221
                  VK P+  +  + D L   + L + EE +    +    V ++    HG+  R  
Sbjct: 236 DSAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEEKIGEGSK----VVVYEGRGHGFVHRPE 295

Query: 222 VEDEEAVKCADEAHGDLLAWFTRYL 239
             +++  + A+EA   +  W   +L
Sbjct: 296 TPEDD--RDAEEAFALMRNWLHHHL 314