Spg031098 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031098
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold11: 10153495 .. 10154412 (+)
RNA-Seq Expression	Spg031098
Synteny	Spg031098

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAGTGGAAACACCCATTGGTGTTACAGATGCGAGCAAACAGTTCGACTTCGTGGGCGAGACATGGTTTGCCCTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGGTGGAAAGCAGTCCCCTTGATTTCTTTGCAGCCAATGGTAATGAATACCACAATCATAGGCTTGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAACGATGTCAGGGGAAGGTTGGAATCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGAGTTCCTGGAATTGGGGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAATGATCGGCGTGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATCACGCAAAGACATGTCCGTTCAAATTCACAATGTCCTGTTTGCCAAGATAAATTCGAATTAGGGTCTGAAGCGAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATAGTTCCCTGGCTAGTCCAGCACAACTCCTGCCCTGTTTGTCGCCAAGAACTGCCTGCACAAGGGTCGAGTAGTAGCCACAGCTCATCTGGAAGCAACAATCTTAGTCGAAATAGCCGAGAGAGGGAGAATACTAGGACGAATCAAGGAAGGAGGAATCCATTTTCGAGTTTTTGGCCATTCCGAGCATCAAGTTCTAACTCTAGCCATAGCACTACTACTGGAAGCAGCTCACCAGCCCTACATGAGAGTAACCAACACCAAACAGGGTATTCCGGATGGCCTTTCGACTAG

mRNA sequence

ATGTCGAGTGGAAACACCCATTGGTGTTACAGATGCGAGCAAACAGTTCGACTTCGTGGGCGAGACATGGTTTGCCCTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGGTGGAAAGCAGTCCCCTTGATTTCTTTGCAGCCAATGGTAATGAATACCACAATCATAGGCTTGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAACGATGTCAGGGGAAGGTTGGAATCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGAGTTCCTGGAATTGGGGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAATGATCGGCGTGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATCACGCAAAGACATGTCCGTTCAAATTCACAATGTCCTGTTTGCCAAGATAAATTCGAATTAGGGTCTGAAGCGAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATAGTTCCCTGGCTAGTCCAGCACAACTCCTGCCCTGTTTGTCGCCAAGAACTGCCTGCACAAGGGTCGAGTAGTAGCCACAGCTCATCTGGAAGCAACAATCTTAGTCGAAATAGCCGAGAGAGGGAGAATACTAGGACGAATCAAGGAAGGAGGAATCCATTTTCGAGTTTTTGGCCATTCCGAGCATCAAGTTCTAACTCTAGCCATAGCACTACTACTGGAAGCAGCTCACCAGCCCTACATGAGAGTAACCAACACCAAACAGGGTATTCCGGATGGCCTTTCGACTAG

Coding sequence (CDS)

ATGTCGAGTGGAAACACCCATTGGTGTTACAGATGCGAGCAAACAGTTCGACTTCGTGGGCGAGACATGGTTTGCCCTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGGTGGAAAGCAGTCCCCTTGATTTCTTTGCAGCCAATGGTAATGAATACCACAATCATAGGCTTGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAACGATGTCAGGGGAAGGTTGGAATCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGAGTTCCTGGAATTGGGGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAATGATCGGCGTGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATCACGCAAAGACATGTCCGTTCAAATTCACAATGTCCTGTTTGCCAAGATAAATTCGAATTAGGGTCTGAAGCGAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATAGTTCCCTGGCTAGTCCAGCACAACTCCTGCCCTGTTTGTCGCCAAGAACTGCCTGCACAAGGGTCGAGTAGTAGCCACAGCTCATCTGGAAGCAACAATCTTAGTCGAAATAGCCGAGAGAGGGAGAATACTAGGACGAATCAAGGAAGGAGGAATCCATTTTCGAGTTTTTGGCCATTCCGAGCATCAAGTTCTAACTCTAGCCATAGCACTACTACTGGAAGCAGCTCACCAGCCCTACATGAGAGTAACCAACACCAAACAGGGTATTCCGGATGGCCTTTCGACTAG

Protein sequence

MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRLGLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYSGWPFD

Homology

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BLAST of Spg031098 vs. NCBI nr
Match: KAG7028452.1 (putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 590.5 bits (1521), Expect = 8.3e-165
Identity = 281/305 (92.13%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MSS NTHWCYRCEQ V+LRGRDMVC SCNGGFVQELDEM+E  PLDFF ANG+EYHN RL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRLESIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NNLSRNSREREN+RTNQGRRNPFSS WPFRASSS+S+HS TTGSSSPALHES QHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. NCBI nr
Match: XP_038904133.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida])

HSP 1 Score: 589.0 bits (1517), Expect = 2.4e-164
Identity = 278/305 (91.15%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MS+GNTHWCYRCEQ VRLRGRDM+CPSCNGGFVQELDEM+E SP D F A+GNEY N RL
Sbjct: 34  MSTGNTHWCYRCEQPVRLRGRDMICPSCNGGFVQELDEMMERSPFDLFEASGNEYQNRRL 93

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRLESIPDHGPGFGPWLIFGGQIPVR+SGHGGFEAFFNG
Sbjct: 94  GLLELFSNFMRQRMVDRNDMRGRLESIPDHGPGFGPWLIFGGQIPVRMSGHGGFEAFFNG 153

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 154 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 213

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 214 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSVSSHSSSGS 273

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NN SRNSR+REN+RT+QGRRNPF+S WPFRASSSNS+H  TTGSSSPALHESNQHQTGY 
Sbjct: 274 NNSSRNSRDRENSRTSQGRRNPFTSIWPFRASSSNSNHGATTGSSSPALHESNQHQTGYY 333

Query: 301 GWPFD 306
           GWPFD
Sbjct: 334 GWPFD 338

[Close]

BLAST of Spg031098 vs. NCBI nr
Match: XP_038904134.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Benincasa hispida])

HSP 1 Score: 589.0 bits (1517), Expect = 2.4e-164
Identity = 278/305 (91.15%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MS+GNTHWCYRCEQ VRLRGRDM+CPSCNGGFVQELDEM+E SP D F A+GNEY N RL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMICPSCNGGFVQELDEMMERSPFDLFEASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRLESIPDHGPGFGPWLIFGGQIPVR+SGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDMRGRLESIPDHGPGFGPWLIFGGQIPVRMSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSVSSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NN SRNSR+REN+RT+QGRRNPF+S WPFRASSSNS+H  TTGSSSPALHESNQHQTGY 
Sbjct: 241 NNSSRNSRDRENSRTSQGRRNPFTSIWPFRASSSNSNHGATTGSSSPALHESNQHQTGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. NCBI nr
Match: XP_023539608.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.2 bits (1515), Expect = 4.1e-164
Identity = 279/305 (91.48%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MSS NTHWCYRCEQ V+LRGRDMVC SCNGGFVQELDEM+E  PLDFF ANG+EYHN RL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRLESIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NNL+RNSREREN+RTNQGRRNPFSS WPFRASSS+S+HS TTGSSSPA+HES QHQTGYS
Sbjct: 241 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. NCBI nr
Match: XP_022940469.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata])

HSP 1 Score: 587.0 bits (1512), Expect = 9.2e-164
Identity = 279/305 (91.48%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MSS NTHWCYRCEQ V+LRGRDMVC SCNGGFVQELDEM+E  PLDFF ANG+EYHN RL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRLESIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELG+EAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS   HSSSGS
Sbjct: 181 HCPVCQDKFELGTEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGIHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NNLSRNSREREN+RTNQGRRNPFSS WPFRASSS+S+HS TTGSSSPALHES QHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.8e-91
Identity = 188/331 (56.80%), Postives = 230/331 (69.49%), Query Frame = 0


Query: 2   SSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAAN-GNEYHNHRL 61
           SS NTHWC+RC++ VRL G++ VC  C GGFV+ELD M ++SP D F ++ G    +   
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DVRGR-LESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   ++RGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HVR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSS 241
           H+R S+S CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G S
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHS--------SSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTG- 301
           SS +         S S++ S NSRE  N      RRNPFSSFWPFR+S S+SS +   G 
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE-----RRNPFSSFWPFRSSGSSSSSTQNRGG 302

Query: 302 --SSSPALHESNQHQ-------TGYSGWPFD 306
             +S  +    N HQ        GYSGWPFD
Sbjct: 303 TRNSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Spg031098 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.4e-77
Identity = 171/329 (51.98%), Postives = 213/329 (64.74%), Query Frame = 0


Query: 5   NTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRLGLLE 64
           NTHWC+RC++ V LR RD VC  C GGFV+E+D       +    A+ +   +    L+E
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEID-------IGPSRAHRDVERDPTFDLME 65

Query: 65  LFSNFMRQRLVDRN---DVRGRLESIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFEAFF 124
            FS FMR RL +R+   ++ GRL S          P LIFGGQ P RL+G  +   EAF 
Sbjct: 66  AFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEAFV 125

Query: 125 NG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQRH 184
           NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KITQ+H
Sbjct: 126 NGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQKH 185

Query: 185 VR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSS 244
           ++ S+S CPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GSSSS
Sbjct: 186 LKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSSSS 245

Query: 245 HSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNS------SHSTTTGSSSPA 304
             S  S N S N RE      N  RRN FS+ WPFR+SSS+S      +++T T      
Sbjct: 246 TQS--SQNRSTNGRE------NSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEEGHY 305

Query: 305 LH-----ESNQHQ-------TGYSGWPFD 306
            H     + +QHQ        GYSGWPFD
Sbjct: 306 HHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of Spg031098 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.0e-37
Identity = 103/296 (34.80%), Postives = 146/296 (49.32%), Query Frame = 0


Query: 8   WCYRCEQTVRLR----GRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNE--YHNHRLG 67
           +CY+C +TV +       D  CP CN GF++E D    +    F   N N   +H+  L 
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 68  LLELFSNFM-------------RQRLVDRND--VRGRLESIPDHGPGFGPWLIFGGQIPV 127
           L + F++ +                 +D N+  + G   S       F P+      +  
Sbjct: 84  LSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLND 143

Query: 128 RLSGHGGFEAFFNGVPGIGVSR--SNGGDYFIGPGLEELFEQLSAND--RRGPPPASRSS 187
             S     E      P     R  +N GDYFIGPGLE+L +QL+ ND  R G PPAS+S+
Sbjct: 144 LRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSA 203

Query: 188 IDAMPVVKITQRHVRSN-SQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPV 247
           I+A+P+V IT+ ++ S  +QC VC D FE G+EA+QMPC H+YH DC++PWL  HNSCPV
Sbjct: 204 IEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPV 263

Query: 248 CRQELPAQGSS------SSHSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRA 272
           CR ELP            +  +SG N+   + +  +       R    S  WPF+A
Sbjct: 264 CRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA 319

[Close]

BLAST of Spg031098 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.6e-36
Identity = 101/301 (33.55%), Postives = 144/301 (47.84%), Query Frame = 0


Query: 8   WCYRCEQTVRL---RGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRLGLLE 67
           +CY+C QTV +      D  CP CN GF++E ++   +  L+F   + + +      + +
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSF----FPMAD 81

Query: 68  LFSNFMRQRLVDRNDVRGRLESIPDHGP----------------GFGPWLIFGGQIPVRL 127
            FS  +             ++ +   GP                 F P+      +    
Sbjct: 82  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLR 141

Query: 128 SGHGGFEAFFNGVPGIGVSR--SNGGDYFIGPGLEELFEQLSAND--RRGPPPASRSSID 187
           S    FE      P    +R   N GDYF GPGLE+L +QL+ ND  R G PPAS+S+ID
Sbjct: 142 SSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAID 201

Query: 188 AMPVVKITQRHVRSN-SQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCR 247
           A+P VK+T+  ++S  +QC VC D+FE GS+ +QMPC H++H DC++PWL  HNSCPVCR
Sbjct: 202 ALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCR 261

Query: 248 QELPAQGSSSSHSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTG 285
            ELP       + S GS          E  +T    R      WPFR    + S S   G
Sbjct: 262 FELPTDDPDYENRSQGSQGSGDGQGSVEGQQT---PRFSIQLPWPFRRQDGSGSGSGAPG 315

[Close]

BLAST of Spg031098 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.2e-30
Identity = 85/260 (32.69%), Postives = 132/260 (50.77%), Query Frame = 0


Query: 6   THWCYRCEQTVRL---RGRDMV----CPSCNGGFVQELDEMVESSPLDFFAANGNEYHNH 65
           ++WCY C + V +   +G   V    CP C+GGF++++++   ++      A+       
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPAS------- 76

Query: 66  RLGLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGG----- 125
                E+ S    +R V R    GR        P F P ++  G    R  G  G     
Sbjct: 77  ----TEVRSINNNRRSVIRRRRSGR-------RPSFNPVIVLQGGAGEREEGEEGDAARD 136

Query: 126 ---FEAFFNGVPGIGVS--RSNGGDYFIGPGLEELFEQLSAND-------RRGPPPASRS 185
              FE +++   G G+     +  +  +G G E L EQLS  +       R G PPAS+S
Sbjct: 137 RRAFEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKS 196

Query: 186 SIDAMPVVKITQRHVRSNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPV 242
           +I+++P V+I+  H+ S + C VC + FE  +EAR+MPC H++H DCIVPWL   NSCPV
Sbjct: 197 AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPV 256

[Close]

BLAST of Spg031098 vs. ExPASy TrEMBL
Match: A0A6J1FK80 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.4e-164
Identity = 279/305 (91.48%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MSS NTHWCYRCEQ V+LRGRDMVC SCNGGFVQELDEM+E  PLDFF ANG+EYHN RL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRLESIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELG+EAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS   HSSSGS
Sbjct: 181 HCPVCQDKFELGTEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGIHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NNLSRNSREREN+RTNQGRRNPFSS WPFRASSS+S+HS TTGSSSPALHES QHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. ExPASy TrEMBL
Match: A0A6J1IB00 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 7.6e-164
Identity = 278/305 (91.15%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MSS NTHWCYRCEQ V+LRGRDMVC SCNGGFVQELDEM+E  PLDFF A+G+EYHN RL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAHGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLL+LFSNFMRQRL+DRND+RGRLESIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLDLFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSR+NGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRNNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NNLSRNSREREN+RTNQGRRNPFSS WPFRASSS+S+HS TTGSSSPALHES QHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. ExPASy TrEMBL
Match: A0A5A7UFI3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006970 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 276/305 (90.49%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MS+GNTHWCYRCEQ VRLRGRDM C SCNGGFVQELDEM+E SPLD F A+GNEY N RL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRL+SIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NN SRNSR+REN+RT QGRRNPF+S WPFRASSSNS+H  TTGS+SPALHE+NQH+TGY 
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. ExPASy TrEMBL
Match: A0A1S3B774 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 276/305 (90.49%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MS+GNTHWCYRCEQ VRLRGRDM C SCNGGFVQELDEM+E SPLD F A+GNEY N RL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRL+SIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NN SRNSR+REN+RT QGRRNPF+S WPFRASSSNS+H  TTGS+SPALHE+NQH+TGY 
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. ExPASy TrEMBL
Match: A0A0A0LCH6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.4e-162
Identity = 276/305 (90.49%), Postives = 288/305 (94.43%), Query Frame = 0


Query: 1   MSSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRL 60
           MS+ NTHWCYRCEQ VRLRGRDM C SCNGGFVQELDEM+E SPLD F A+GNEY N RL
Sbjct: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDVRGRLESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRLVDRND+RGRL+SIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHVRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRH+RSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 QCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240
            CPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240

Query: 241 NNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTGSSSPALHESNQHQTGYS 300
           NN SRNSR+REN RT QGRRNPF+S WPFRASSSNS+HS TTGS+SPALHE+NQH+TGY 
Sbjct: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Spg031098 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 336.7 bits (862), Expect = 2.0e-92
Identity = 188/331 (56.80%), Postives = 230/331 (69.49%), Query Frame = 0


Query: 2   SSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAAN-GNEYHNHRL 61
           SS NTHWC+RC++ VRL G++ VC  C GGFV+ELD M ++SP D F ++ G    +   
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DVRGR-LESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   ++RGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HVR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSS 241
           H+R S+S CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G S
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHS--------SSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTG- 301
           SS +         S S++ S NSRE  N      RRNPFSSFWPFR+S S+SS +   G 
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE-----RRNPFSSFWPFRSSGSSSSSTQNRGG 302

Query: 302 --SSSPALHESNQHQ-------TGYSGWPFD 306
             +S  +    N HQ        GYSGWPFD
Sbjct: 303 TRNSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Spg031098 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 336.7 bits (862), Expect = 2.0e-92
Identity = 188/331 (56.80%), Postives = 230/331 (69.49%), Query Frame = 0


Query: 2   SSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAAN-GNEYHNHRL 61
           SS NTHWC+RC++ VRL G++ VC  C GGFV+ELD M ++SP D F ++ G    +   
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DVRGR-LESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   ++RGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HVR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSS 241
           H+R S+S CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G S
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHS--------SSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTG- 301
           SS +         S S++ S NSRE  N      RRNPFSSFWPFR+S S+SS +   G 
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE-----RRNPFSSFWPFRSSGSSSSSTQNRGG 302

Query: 302 --SSSPALHESNQHQ-------TGYSGWPFD 306
             +S  +    N HQ        GYSGWPFD
Sbjct: 303 TRNSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Spg031098 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 336.7 bits (862), Expect = 2.0e-92
Identity = 188/331 (56.80%), Postives = 230/331 (69.49%), Query Frame = 0


Query: 2   SSGNTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAAN-GNEYHNHRL 61
           SS NTHWC+RC++ VRL G++ VC  C GGFV+ELD M ++SP D F ++ G    +   
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DVRGR-LESIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   ++RGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HVR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSS 241
           H+R S+S CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G S
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHS--------SSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNSSHSTTTG- 301
           SS +         S S++ S NSRE  N      RRNPFSSFWPFR+S S+SS +   G 
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE-----RRNPFSSFWPFRSSGSSSSSTQNRGG 302

Query: 302 --SSSPALHESNQHQ-------TGYSGWPFD 306
             +S  +    N HQ        GYSGWPFD
Sbjct: 303 TRNSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Spg031098 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.7e-79
Identity = 171/329 (51.98%), Postives = 213/329 (64.74%), Query Frame = 0


Query: 5   NTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRLGLLE 64
           NTHWC+RC++ V LR RD VC  C GGFV+E+D       +    A+ +   +    L+E
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEID-------IGPSRAHRDVERDPTFDLME 65

Query: 65  LFSNFMRQRLVDRN---DVRGRLESIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFEAFF 124
            FS FMR RL +R+   ++ GRL S          P LIFGGQ P RL+G  +   EAF 
Sbjct: 66  AFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEAFV 125

Query: 125 NG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQRH 184
           NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KITQ+H
Sbjct: 126 NGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQKH 185

Query: 185 VR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSS 244
           ++ S+S CPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GSSSS
Sbjct: 186 LKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSSSS 245

Query: 245 HSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNS------SHSTTTGSSSPA 304
             S  S N S N RE      N  RRN FS+ WPFR+SSS+S      +++T T      
Sbjct: 246 TQS--SQNRSTNGRE------NSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEEGHY 305

Query: 305 LH-----ESNQHQ-------TGYSGWPFD 306
            H     + +QHQ        GYSGWPFD
Sbjct: 306 HHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of Spg031098 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 291.2 bits (744), Expect = 9.7e-79
Identity = 171/329 (51.98%), Postives = 213/329 (64.74%), Query Frame = 0


Query: 5   NTHWCYRCEQTVRLRGRDMVCPSCNGGFVQELDEMVESSPLDFFAANGNEYHNHRLGLLE 64
           NTHWC+RC++ V LR RD VC  C GGFV+E+D       +    A+ +   +    L+E
Sbjct: 6   NTHWCHRCQRAVWLRARDAVCSYCGGGFVEEID-------IGPSRAHRDVERDPTFDLME 65

Query: 65  LFSNFMRQRLVDRN---DVRGRLESIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFEAFF 124
            FS FMR RL +R+   ++ GRL S          P LIFGGQ P RL+G  +   EAF 
Sbjct: 66  AFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEAFV 125

Query: 125 NG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQRH 184
           NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KITQ+H
Sbjct: 126 NGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQKH 185

Query: 185 VR-SNSQCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSS 244
           ++ S+S CPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GSSSS
Sbjct: 186 LKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSSSS 245

Query: 245 HSSSGSNNLSRNSRERENTRTNQGRRNPFSSFWPFRASSSNS------SHSTTTGSSSPA 304
             S  S N S N RE      N  RRN FS+ WPFR+SSS+S      +++T T      
Sbjct: 246 TQS--SQNRSTNGRE------NSRRRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEEGHY 305

Query: 305 LH-----ESNQHQ-------TGYSGWPFD 306
            H     + +QHQ        GYSGWPFD
Sbjct: 306 HHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

The following BLAST results are available for this feature:

BLAST of Spg031098 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028452.1	8.3e-165	92.13	putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subs...	[more]
XP_038904133.1	2.4e-164	91.15	probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida]	[more]
XP_038904134.1	2.4e-164	91.15	probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Benincasa hispida]	[more]
XP_023539608.1	4.1e-164	91.48	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo]	[more]
XP_022940469.1	9.2e-164	91.48	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata]	[more]

BLAST of Spg031098 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	2.8e-91	56.80	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	1.4e-77	51.98	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
P0CH30	4.0e-37	34.80	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	2.6e-36	33.55	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q940T5	6.2e-30	32.69	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of Spg031098 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FK80	4.4e-164	91.48	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055...	[more]
A0A6J1IB00	7.6e-164	91.15	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 P...	[more]
A0A5A7UFI3	2.2e-163	90.49	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B774	2.2e-163	90.49	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 ...	[more]
A0A0A0LCH6	1.4e-162	90.49	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE...	[more]

BLAST of Spg031098 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40830.2	2.0e-92	56.80	RING-H2 finger C1A	[more]
AT2G40830.1	2.0e-92	56.80	RING-H2 finger C1A	[more]
AT2G40830.3	2.0e-92	56.80	RING-H2 finger C1A	[more]
AT3G56580.2	9.7e-79	51.98	RING/U-box superfamily protein	[more]
AT3G56580.1	9.7e-79	51.98	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 182..222 e-value: 7.1E-7 score: 38.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 181..223 e-value: 4.2E-12 score: 46.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 182..223 score: 12.120453
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 5..36 e-value: 2.9E-11 score: 43.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 144..229 e-value: 1.3E-22 score: 81.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 257..305
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 226..305
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 226..246
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 2..301
None	No IPR available	PANTHER	PTHR15710:SF83	E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATED	coord: 2..301
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 181..223 e-value: 4.8238E-24 score: 90.0696
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 162..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031098.1	Spg031098.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity