Spg031080 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031080
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold11: 9981519 .. 9982662 (-)
RNA-Seq Expression	Spg031080
Synteny	Spg031080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTGAATGCAGACGCCTCTTGGTTTGCCTCTTCGAATTCTGGTGGGTTGGGTTGGGTGATTCGTGACTCCACAGGATCTCTTCGGGCAGGTGGGTCTTTGAAGATATATTCGTGCAACTCAATCGTGTTTATCGAAGCTTGCGCGATCAGAGAGGGTATGAGAAATTACCTTTCGATGTTCAATTTTGATCCCCCAGACCTGATTGTTGAGTCCGACTCCAAGGACCTGATCAATTTGCTCAATCGATCGGCGATTGATCTCTCCGAATCCCTATCTGTGGTGGAGGATATTTTTGTTCTGTCTTTGAAGATTGGGGGAGTTTCTTTTCGTTGGTGCCCCAGATTGGTTAACCGCGCAACCCACGTTCTAGCGCGCGAAGTGATAACTTGCAAAAAGCAAGTTATTTATCCTTTAAACTTAAGCTAAAACAAGGCCAAAAGAACCAATGGTGCGTTACTTCAGCGCAAAAAGAAAGGAAAATGTGATAATTTTATGCGGCGAGCTATATTCATGTATGCGGTAAGAAATTATAAAATATAGGGCGCTAACGCAACTAATTCAGTTGTTTTCCAAAGTTTGTTCTCAGCGCAGACACACATCCCAAGACGATCAAGAGACAGACAAGACGGATGAAAGGACAACGCTGACACAACTAACGCAAGTAGGCGGAAAATGGGCTGAAAAGCTACAAGTTGGTTGATGATGATTGAAGCAAGTGGCTGAAATACTTGGAGAAGTTAGCTTCCCTCGCCTATAAATAGAAGCCACATACTCATAATGAAGGGGGAGAAAAAAAAGAGGGGGAAGAAAATTGGAGAGACGAAGAGAAGAGGAGAAAGACGAAGAAGAAAAATCACAGCGAGAGCTGTGATTAGGAGCAAGCTTGCGTGTGTAATGTTTTATTTCATGATGTCTTGTTTTGCTCGCATGAGCTAATTTCTGCTTCAAGGCTTAGCGCAAGCACCTCATGCAGTGATAAGTATATTTTGTAAGCGCATATTTTGATTTAAATGAAACATTAGTTGCTTTCTACTTGGTTGTGATACTTTTTCCATTACTAACTCTCGCTTGGCCAGCCGGATGCGGTAGCAGTATGCTAAATCCCTGACAGGTAACGCATGCGGACATCTCTAAGTAG

mRNA sequence

ATGAAGCTGAATGCAGACGCCTCTTGGTTTGCCTCTTCGAATTCTGGTGGGTTGGGTTGGGTGATTCGTGACTCCACAGGATCTCTTCGGGCAGGTGGGTCTTTGAAGATATATTCGTGCAACTCAATCGTGTTTATCGAAGCTTGCGCGATCAGAGAGGGTATGAGAAATTACCTTTCGATGTTCAATTTTGATCCCCCAGACCTGATTGTTGAGTCCGACTCCAAGGACCTGATCAATTTGCTCAATCGATCGGCGATTGATCTCTCCGAATCCCTATCTGTGGTGGAGGATATTTTTGTTCTGTCTTTGAAGATTGGGGGACGCAGACACACATCCCAAGACGATCAAGAGACAGACAAGACGGATGAAAGGACAACGCTGACACAACTAACGCAAGTAACGCATGCGGACATCTCTAAGTAG

Coding sequence (CDS)

ATGAAGCTGAATGCAGACGCCTCTTGGTTTGCCTCTTCGAATTCTGGTGGGTTGGGTTGGGTGATTCGTGACTCCACAGGATCTCTTCGGGCAGGTGGGTCTTTGAAGATATATTCGTGCAACTCAATCGTGTTTATCGAAGCTTGCGCGATCAGAGAGGGTATGAGAAATTACCTTTCGATGTTCAATTTTGATCCCCCAGACCTGATTGTTGAGTCCGACTCCAAGGACCTGATCAATTTGCTCAATCGATCGGCGATTGATCTCTCCGAATCCCTATCTGTGGTGGAGGATATTTTTGTTCTGTCTTTGAAGATTGGGGGACGCAGACACACATCCCAAGACGATCAAGAGACAGACAAGACGGATGAAAGGACAACGCTGACACAACTAACGCAAGTAACGCATGCGGACATCTCTAAGTAG

Protein sequence

MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLSMFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVLSLKIGGRRHTSQDDQETDKTDERTTLTQLTQVTHADISK

Homology

[Close]

BLAST of Spg031080 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 76.6 bits (187), Expect = 1.9e-10
Identity = 42/100 (42.00%), Postives = 65/100 (65.00%), Query Frame = 0


Query: 3   LNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLSMF 62
           LNADASW  S++ GG+GW+IR   G +   G+  + +CN++  +EA AI EG+RN  ++ 
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 63  NFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL 103
              P  L +E+DS ++ +LLNR   DL+++  VVE+I  L
Sbjct: 141 VLRP--LHIETDSAEVESLLNRKREDLTKTGWVVEEILNL 178

[Close]

BLAST of Spg031080 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 64.7 bits (156), Expect = 7.3e-07
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 2   KLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMR----- 61
           KLN DA+W A +N+GG+GW++RD  G +       I +  +I ++E  AI EG+R     
Sbjct: 88  KLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQE 147

Query: 62  --NYLSMFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL--SLKIGGRRHT 116
               +   +  P  + +ESDS + I+LL+R   D +E + ++E+I+ +   +KI   RH 
Sbjct: 148 HCRPIQQEHCRP--IHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 207

[Close]

BLAST of Spg031080 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-06
Identity = 38/116 (32.76%), Postives = 69/116 (59.48%), Query Frame = 0


Query: 2   KLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLSM 61
           KLN +A+W A +N+GG+GW++RD  G +       I +  +I ++E  AI EG+R   ++
Sbjct: 88  KLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLR---AI 147

Query: 62  FNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL--SLKIGGRRHTSQD 116
                  + +ESDS + I+LL+R   D +E + ++E+I+ +   ++I   RH S++
Sbjct: 148 RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISRE 200

[Close]

BLAST of Spg031080 vs. NCBI nr
Match: BBH00303.1 (Ribonuclease H-like superfamily protein [Prunus dulcis])

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-06
Identity = 38/103 (36.89%), Postives = 55/103 (53.40%), Query Frame = 0


Query: 1   MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLS 60
           +KLN D +W A +  GG+GWV+RD  G   + G      C S +  EA A+RE +   L 
Sbjct: 450 VKLNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEAVREALTWCLE 509

Query: 61  MFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVLS 104
           +  FD   + VESDS  LI +LN+  +   E   ++ D+  LS
Sbjct: 510 L-GFD--KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLS 549

[Close]

BLAST of Spg031080 vs. NCBI nr
Match: CAB4274760.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 62.0 bits (149), Expect = 4.7e-06
Identity = 40/99 (40.40%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 1    MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLS 60
            +K+N DA+W A    GG+GWVIRDS G L   G       +S + +E  AIR  +     
Sbjct: 1135 LKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAIRHALS---E 1194

Query: 61   MFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDI 100
              NF   D+IVESDS+  I +LN  A   S+   +V DI
Sbjct: 1195 CANFHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDI 1230

[Close]

BLAST of Spg031080 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 9.0e-11
Identity = 42/100 (42.00%), Postives = 65/100 (65.00%), Query Frame = 0


Query: 3   LNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLSMF 62
           LNADASW  S++ GG+GW+IR   G +   G+  + +CN++  +EA AI EG+RN  ++ 
Sbjct: 81  LNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAILEGLRNLTNLG 140

Query: 63  NFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL 103
              P  L +E+DS ++ +LLNR   DL+++  VVE+I  L
Sbjct: 141 VLRP--LHIETDSAEVESLLNRKREDLTKTGWVVEEILNL 178

[Close]

BLAST of Spg031080 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.5e-07
Identity = 41/123 (33.33%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 2   KLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMR----- 61
           KLN DA+W A +N+GG+GW++RD  G +       I +  +I ++E  AI EG+R     
Sbjct: 88  KLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQE 147

Query: 62  --NYLSMFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL--SLKIGGRRHT 116
               +   +  P  + +ESDS + I+LL+R   D +E + ++E+I+ +   +KI   RH 
Sbjct: 148 HCRPIQQEHCRP--IHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHI 207

[Close]

BLAST of Spg031080 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 6.0e-07
Identity = 38/116 (32.76%), Postives = 69/116 (59.48%), Query Frame = 0


Query: 2   KLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLSM 61
           KLN +A+W A +N+GG+GW++RD  G +       I +  +I ++E  AI EG+R   ++
Sbjct: 88  KLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLR---AI 147

Query: 62  FNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVL--SLKIGGRRHTSQD 116
                  + +ESDS + I+LL+R   D +E + ++E+I+ +   ++I   RH S++
Sbjct: 148 RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISRE 200

[Close]

BLAST of Spg031080 vs. ExPASy TrEMBL
Match: A0A4Y1R838 (Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-06
Identity = 38/103 (36.89%), Postives = 55/103 (53.40%), Query Frame = 0


Query: 1   MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLS 60
           +KLN D +W A +  GG+GWV+RD  G   + G      C S +  EA A+RE +   L 
Sbjct: 450 VKLNCDGAWVAQTGRGGVGWVLRDGIGCFISAGGCGDMRCTSALMAEAEAVREALTWCLE 509

Query: 61  MFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDIFVLS 104
           +  FD   + VESDS  LI +LN+  +   E   ++ D+  LS
Sbjct: 510 L-GFD--KVAVESDSLSLIKMLNKEDVVDLEIEGILFDVHCLS 549

[Close]

BLAST of Spg031080 vs. ExPASy TrEMBL
Match: A0A6J5WPU6 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS20372 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.3e-06
Identity = 40/99 (40.40%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 1   MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLS 60
           +K+N DA+W A    GG+GWVIRDS G L   G       +S + +E  AIR  +     
Sbjct: 804 LKVNCDAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAIRHALS---E 863

Query: 61  MFNFDPPDLIVESDSKDLINLLNRSAIDLSESLSVVEDI 100
             NF   D+IVESDS+  I +LN  A   S+   +V DI
Sbjct: 864 CANFHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDI 899

[Close]

BLAST of Spg031080 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 8.6e-06
Identity = 29/87 (33.33%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 1   MKLNADASWFASSNSGGLGWVIRDSTGSLRAGGSLKIYSCNSIVFIEACAIREGMRNYLS 60
           +K N DA+W   +   G+GWV+R+  G ++  G+  +    S++  E  A+R  +   LS
Sbjct: 428 VKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAV---LS 487

Query: 61  MFNFDPPDLIVESDSKDLINLLNRSAI 88
           +  F    +I ESDS+ LI +LN   I
Sbjct: 488 LSRFQYNYVIFESDSQVLIEILNNDEI 511

The following BLAST results are available for this feature:

BLAST of Spg031080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.9e-10	42.00	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022156777.1	7.3e-07	33.33	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	1.2e-06	32.76	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
BBH00303.1	2.1e-06	36.89	Ribonuclease H-like superfamily protein [Prunus dulcis]	[more]
CAB4274760.1	4.7e-06	40.40	unnamed protein product [Prunus armeniaca]	[more]

BLAST of Spg031080 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg031080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	9.0e-11	42.00	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DSV1	3.5e-07	33.33	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	6.0e-07	32.76	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A4Y1R838	1.0e-06	36.89	Ribonuclease H-like superfamily protein OS=Prunus dulcis OX=3755 GN=Prudu_010255...	[more]
A0A6J5WPU6	2.3e-06	40.40	Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=...	[more]

BLAST of Spg031080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	8.6e-06	33.33	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..135 e-value: 5.0E-5 score: 25.4
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 4..101 e-value: 9.9E-11 score: 41.5
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 1..110
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 3..107 e-value: 9.22894E-16 score: 66.5688
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 1..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031080.1	Spg031080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity