Spg031013 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031013
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Serine proteinase inhibitor
Location	scaffold10: 22592436 .. 22593165 (+)
RNA-Seq Expression	Spg031013
Synteny	Spg031013

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATTAATCCTTTGGCATTGCATTATCCAAACTCCTTCACACAAACCCAAATCCTTCAATCATCTTCAACTATGGCTGATGTTACTTCTCTTGATCTTGGTATGCTTCAATTTCTTCTTTTTCTTTACTACTTCGTTCTCCGTTTTTTACATTATTCAAACCTTCGATCACGAGTTCACATTCTTTTTCCTCGAATTGTTGTCTTTGTTTTCATAACGATTTTTTTACAAGTATGACTTGTTGAAATTATCATTCATTATTTTATGAACCAATAATTTTAACAAGGAGAATATCTTTATTTGTTTTGAAAATTTGGTTATTTATGGTTTGTTTGTTTATTAGGGAAAGAACGTTGGCCGGAACTTGTTGGAATAAAGTCAACCACTGCAAAGTACGTTATCCAGAAAGATAATCCTAAAGTGAAACTATTTGAGATATACTTGGCAGGAACGGGAGTGCATGAAGACTTTGCGATTAATCGAGTTCGGTTGTTTGTTAATATAGAAGATGTGGTGGTTCAAATTCCGACAATTGGTTAGAGTCTAAAAGTGTTTGTATTTTTATTCACAAAATGGTTCCTAAACTATGTAAGCCAATAAGGTAGAACATCTTCTACCACTTCAATTAATCCCTTTTTCTTTCAATTGTCTTTCTTTATGTATTATTGAACCCATCTCTTGAATGGCGTTTCAATTTGTTTTCCCTTTCATTATTGTTGAAGAATTTGCAG

mRNA sequence

ATGGCTGATGTTACTTCTCTTGATCTTGGGAAAGAACGTTGGCCGGAACTTGTTGGAATAAAGTCAACCACTGCAAAGTACGTTATCCAGAAAGATAATCCTAAAGTGAAACTATTTGAGATATACTTGGCAGGAACGGGAGTGCATGAAGACTTTGCGATTAATCGAGTTCGGTTGTTTGTTAATATAGAAGATGTGGTGGTTCAAATTCCGACAATTGGTTAG

Coding sequence (CDS)

ATGGCTGATGTTACTTCTCTTGATCTTGGGAAAGAACGTTGGCCGGAACTTGTTGGAATAAAGTCAACCACTGCAAAGTACGTTATCCAGAAAGATAATCCTAAAGTGAAACTATTTGAGATATACTTGGCAGGAACGGGAGTGCATGAAGACTTTGCGATTAATCGAGTTCGGTTGTTTGTTAATATAGAAGATGTGGTGGTTCAAATTCCGACAATTGGTTAG

Protein sequence

MADVTSLDLGKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQIPTIG

Homology

[Close]

BLAST of Spg031013 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-13
Identity = 40/65 (61.54%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK RWPELVGIKST A+++I+KDN  V+  E  LAG+ V ED   +RVR+FVNI+ ++VQ
Sbjct: 8  GKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKGIIVQ 67

Query: 70 IPTIG 75
          IPTIG
Sbjct: 68 IPTIG 72

[Close]

BLAST of Spg031013 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 85.5 bits (210), Expect = 2.1e-13
Identity = 37/65 (56.92%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK +WPELVG+K+TTAKY+I+KDNP V+   + LAG+G  ED   +RV +FVNI D+VV+
Sbjct: 8  GKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLVVE 67

Query: 70 IPTIG 75
          +P +G
Sbjct: 68 VPKVG 72

[Close]

BLAST of Spg031013 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-13
Identity = 39/65 (60.00%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK +WPELVGIK+TTA+Y+I+KDNP V+   + LAGTG  +D   +RV LFVNIE+ VV+
Sbjct: 8  GKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEEKVVE 67

Query: 70 IPTIG 75
          IP +G
Sbjct: 68 IPKVG 72

[Close]

BLAST of Spg031013 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 80.1 bits (196), Expect = 8.8e-12
Identity = 39/74 (52.70%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 1  MADVTSLDLGKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLF 60
          MAD+   +  K +WPELVGIK+T A+Y+I+KDNP V+   + LAGTG  +DF  +RV LF
Sbjct: 1  MADICRPE--KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLF 60

Query: 61 VNIEDVVVQIPTIG 75
           NI+  VV++PT+G
Sbjct: 61 TNIDGRVVEMPTVG 72

[Close]

BLAST of Spg031013 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 79.0 bits (193), Expect = 2.0e-11
Identity = 37/65 (56.92%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK RWPELVGI++   + +I+K+NP V + +I+LAG+ V  DF+ +RVRLFVNI DVVV 
Sbjct: 8  GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67

Query: 70 IPTIG 75
          IP  G
Sbjct: 68 IPWTG 72

[Close]

BLAST of Spg031013 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.4e-12
Identity = 35/64 (54.69%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 11  KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
           KERWPEL+G  +  A  +IQK+NPK+   +  L GT V ED   NRVRLFVN+ D VVQ 
Sbjct: 44  KERWPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPVTEDLRCNRVRLFVNVLDFVVQT 103

Query: 71  PTIG 75
           P +G
Sbjct: 104 PQVG 107

[Close]

BLAST of Spg031013 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 3.2e-12
Identity = 34/64 (53.12%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 11  KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
           KE WPEL+G  +  AK +IQK+NPK+   E  L G+   ED   NRVRLFVN+ D+VVQ 
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

Query: 71  PTIG 75
           P +G
Sbjct: 116 PKVG 119

[Close]

BLAST of Spg031013 vs. ExPASy Swiss-Prot
Match: Q03198 (Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.6e-11
Identity = 34/64 (53.12%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 11  KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
           KERWPEL+G  +  A  +IQK+NPK+   +  L G  V ED   NRVRLFVN+ D +VQ 
Sbjct: 44  KERWPELLGTPAKFAMQIIQKENPKLTNVQTILNGGPVTEDLRCNRVRLFVNVLDFIVQT 103

Query: 71  PTIG 75
           P IG
Sbjct: 104 PQIG 107

[Close]

BLAST of Spg031013 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 7.8e-11
Identity = 32/64 (50.00%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 11 KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
          KE WPEL+G+ +  A+ +IQK+N K+      L G+ V +DF   RVRLFVN+ D VVQI
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90

Query: 71 PTIG 75
          P +G
Sbjct: 91 PRVG 94

[Close]

BLAST of Spg031013 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 32/64 (50.00%), Postives = 44/64 (68.75%), Query Frame = 0


Query: 10  GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
           GK+ WPEL+G+ +  AK +I+K+NP +    I L G+ V +DF  +RVRLFVNI   VVQ
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 70  IPTI 74
           IP +
Sbjct: 107 IPRV 110

[Close]

BLAST of Spg031013 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.0e-13
Identity = 37/65 (56.92%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK +WPELVG+K+TTAKY+I+KDNP V+   + LAG+G  ED   +RV +FVNI D+VV+
Sbjct: 8  GKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLVVE 67

Query: 70 IPTIG 75
          +P +G
Sbjct: 68 VPKVG 72

[Close]

BLAST of Spg031013 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.3e-13
Identity = 39/65 (60.00%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQ 69
          GK +WPELVGIK+TTA+Y+I+KDNP V+   + LAGTG  +D   +RV LFVNIE+ VV+
Sbjct: 8  GKNQWPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTGTTKDIRCDRVWLFVNIEEKVVE 67

Query: 70 IPTIG 75
          IP +G
Sbjct: 68 IPKVG 72

[Close]

BLAST of Spg031013 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.3e-12
Identity = 39/74 (52.70%), Postives = 54/74 (72.97%), Query Frame = 0


Query: 1  MADVTSLDLGKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLF 60
          MAD+   +  K +WPELVGIK+T A+Y+I+KDNP V+   + LAGTG  +DF  +RV LF
Sbjct: 1  MADICRPE--KNQWPELVGIKATIAEYIIKKDNPNVENVIVLLAGTGTTKDFRSDRVWLF 60

Query: 61 VNIEDVVVQIPTIG 75
           NI+  VV++PT+G
Sbjct: 61 TNIDGRVVEMPTVG 72

[Close]

BLAST of Spg031013 vs. ExPASy TrEMBL
Match: Q40444 (Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX=79230 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-10
Identity = 35/64 (54.69%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 11 KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
          KE WPEL+G+ +  A+ +IQK+NPK+      L G+ V +DF  NRVRLFVN+ DVVVQI
Sbjct: 1  KETWPELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQI 60

Query: 71 PTIG 75
          P +G
Sbjct: 61 PRVG 64

[Close]

BLAST of Spg031013 vs. ExPASy TrEMBL
Match: A0A6J1HFK9 (inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LOC111463862 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.2e-10
Identity = 35/67 (52.24%), Postives = 49/67 (73.13%), Query Frame = 0


Query: 10 GKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAG--TGVHEDFAINRVRLFVNIEDVV 69
          GK RWPELVGI++  A+ +IQK+NP V + +I+L G  +G+  +F  +RVRL VNI  V+
Sbjct: 8  GKNRWPELVGIEARIAEKIIQKENPNVDIVQIFLFGSKSGITMEFCCSRVRLLVNINGVI 67

Query: 70 VQIPTIG 75
          V IP+IG
Sbjct: 68 VGIPSIG 74

[Close]

BLAST of Spg031013 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-10
Identity = 35/67 (52.24%), Postives = 44/67 (65.67%), Query Frame = 0


Query: 8  DLGKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVV 67
          D GK  WPEL+G K   AK VI+++NPK+K   I L GT V E F  +RV ++VN   +V
Sbjct: 34 DPGKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGIV 93

Query: 68 VQIPTIG 75
          VQIP IG
Sbjct: 94 VQIPIIG 99

[Close]

BLAST of Spg031013 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.9 bits (115), Expect = 2.0e-06
Identity = 26/64 (40.62%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 11 KERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVVVQI 70
          K  WPEL G     A  VI+++NP V    + L G+ V  DF  +RVR+FV+   +VV+ 
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNA-AVILDGSPVTADFRCDRVRVFVDGNRIVVKT 67

Query: 71 PTIG 75
          P  G
Sbjct: 68 PKSG 70

[Close]

BLAST of Spg031013 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 45.1 bits (105), Expect = 2.9e-05
Identity = 25/67 (37.31%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 8  DLGKERWPELVGIKSTTAKYVIQKDNPKVKLFEIYLAGTGVHEDFAINRVRLFVNIEDVV 67
          D+GK  WPEL+G     A  VI+ +N  + +  +   G  V ED +  RVR++V+   +V
Sbjct: 23 DIGKNSWPELLGTNGDYAASVIKGENSSLNVV-VVSDGNYVTEDLSCYRVRVWVDEIRIV 82

Query: 68 VQIPTIG 75
          V+ PT G
Sbjct: 83 VRNPTAG 88

The following BLAST results are available for this feature:

BLAST of Spg031013 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571494.1	1.6e-13	61.54	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN50840.1	2.1e-13	56.92	hypothetical protein Csa_004718 [Cucumis sativus]	[more]
ALD47589.1	2.7e-13	60.00	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50838.1	8.8e-12	52.70	hypothetical protein Csa_004692 [Cucumis sativus]	[more]
KAG6571499.1	2.0e-11	56.92	Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg031013 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q03199	2.4e-12	54.69	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]
P20076	3.2e-12	53.13	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q03198	1.6e-11	53.13	Proteinase inhibitor I-A OS=Nicotiana tabacum OX=4097 GN=TIMPB PE=2 SV=1	[more]
Q02214	7.8e-11	50.00	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P16231	1.3e-10	50.00	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]

BLAST of Spg031013 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KME7	1.0e-13	56.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]
A0A0M3SAG8	1.3e-13	60.00	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KMM0	4.3e-12	52.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
Q40444	1.8e-10	54.69	Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX...	[more]
A0A6J1HFK9	5.2e-10	52.24	inhibitor of trypsin and hageman factor-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Spg031013 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43580.1	1.4e-10	52.24	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	2.0e-06	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	2.9e-05	37.31	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 51..61 score: 60.87 coord: 13..26 score: 50.31
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 11..74 e-value: 1.9E-21 score: 76.5
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 9..74
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 14..25
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 3..74 e-value: 2.6E-22 score: 80.8
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 9..74
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 9..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031013.1	Spg031013.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity