Spg030266 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030266
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionEF hand calcium-binding protein family
Locationscaffold6: 15451827 .. 15452099 (+)
RNA-Seq ExpressionSpg030266
SyntenySpg030266
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCGCAACAGCAAAACATACCAGCAGATGCCGAAGCTGTCAAGGGAGCAACTAAGCCTAATCTTCAGAAACCACGATGACGACGGCGACGGCCGCCTCAATACCTCTGAGCTAACCGAAGCCTTCCGGTACATCGGCGCCCTTCTTCCATCGTATAGAGCACGCCGCGCTCTTCTTCACTCCGATTCCGATAGAGACGGATTCATCGGCGAGAAGGATTTCGAATTGCTCATCAATTATGCCCACAAACGCAAATACACCGTCATGTGA

mRNA sequence

ATGCCTCGCAACAGCAAAACATACCAGCAGATGCCGAAGCTGTCAAGGGAGCAACTAAGCCTAATCTTCAGAAACCACGATGACGACGGCGACGGCCGCCTCAATACCTCTGAGCTAACCGAAGCCTTCCGGTACATCGGCGCCCTTCTTCCATCGTATAGAGCACGCCGCGCTCTTCTTCACTCCGATTCCGATAGAGACGGATTCATCGGCGAGAAGGATTTCGAATTGCTCATCAATTATGCCCACAAACGCAAATACACCGTCATGTGA

Coding sequence (CDS)

ATGCCTCGCAACAGCAAAACATACCAGCAGATGCCGAAGCTGTCAAGGGAGCAACTAAGCCTAATCTTCAGAAACCACGATGACGACGGCGACGGCCGCCTCAATACCTCTGAGCTAACCGAAGCCTTCCGGTACATCGGCGCCCTTCTTCCATCGTATAGAGCACGCCGCGCTCTTCTTCACTCCGATTCCGATAGAGACGGATTCATCGGCGAGAAGGATTTCGAATTGCTCATCAATTATGCCCACAAACGCAAATACACCGTCATGTGA

Protein sequence

MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDFELLINYAHKRKYTVM
Homology
BLAST of Spg030266 vs. NCBI nr
Match: KAG7024248.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.4 bits (381), Expect = 3.8e-33
Identity = 73/90 (81.11%), Postives = 79/90 (87.78%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SKTYQ  PKLSREQLS+IFRNHD DGDGRL+T+ELT AFRYIGA LPSYRA  ALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          H+DSD DGFIGEKDFELL+NYA KRKYTV+
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYTVI 90

BLAST of Spg030266 vs. NCBI nr
Match: KAG6591370.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 149.4 bits (376), Expect = 1.4e-32
Identity = 72/88 (81.82%), Postives = 77/88 (87.50%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SKTYQ  PKLSREQLS+IFRNHD DGDGRL+T+ELT AFRYIGA LPSYRA  ALL
Sbjct: 1  MARKSKTYQPRPKLSREQLSVIFRNHDGDGDGRLSTTELTAAFRYIGAFLPSYRALHALL 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYT 89
          H+DSD DGFIGEKDFELL+NYA KRKYT
Sbjct: 61 HTDSDGDGFIGEKDFELLVNYAEKRKYT 88

BLAST of Spg030266 vs. NCBI nr
Match: KGN55185.1 (hypothetical protein Csa_012915 [Cucumis sativus])

HSP 1 Score: 141.0 bits (354), Expect = 5.1e-30
Identity = 67/90 (74.44%), Postives = 76/90 (84.44%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SK Y    KLSREQL LIFRNHD D DGRL+T+ELTEAFRYIGA LP+YRAR ALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          H+DSDRDGFIGEKDFE L+NYA +R+YT++
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

BLAST of Spg030266 vs. NCBI nr
Match: KAA0064376.1 (putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 140.2 bits (352), Expect = 8.7e-30
Identity = 67/90 (74.44%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SKTY    +LSREQL LIFRNHD D DGRL+T+ELTEAFRYIGA LP YRAR AL 
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          H+DSDRDGFI EKDFELL+NYA +R+YTV+
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

BLAST of Spg030266 vs. NCBI nr
Match: KAG6600744.1 (Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 139.0 bits (349), Expect = 1.9e-29
Identity = 65/90 (72.22%), Postives = 79/90 (87.78%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          MPR +KTY   P++SREQL LIFRNHDD+GDGRL+++ELT+AF YIGALLPS+RA  ALL
Sbjct: 1  MPRKTKTYHPRPRISREQLRLIFRNHDDNGDGRLSSAELTQAFHYIGALLPSFRACHALL 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          ++DSDRDGFI EKDFE LI+YA KR+YTV+
Sbjct: 61 YTDSDRDGFIDEKDFEQLIDYAEKRQYTVI 90

BLAST of Spg030266 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 5.4e-06
Identity = 23/58 (39.66%), Postives = 34/58 (58.62%), Query Frame = 0

Query: 18 QLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDF 76
          +L  +FR +D +GDG+++  EL    R +GA L     RR +   DSDRDGF+   +F
Sbjct: 5  ELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

BLAST of Spg030266 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 2.0e-05
Identity = 25/72 (34.72%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 4   NSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSD 63
           N K++Q     S+E+L  +F + D DGDG+++  EL   F  +G  +    A+ A+   D
Sbjct: 54  NIKSHQ----ASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVD 113

Query: 64  SDRDGFIGEKDF 76
           +D DG +G +DF
Sbjct: 114 TDADGSLGFEDF 121

BLAST of Spg030266 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 5.9e-05
Identity = 21/64 (32.81%), Postives = 35/64 (54.69%), Query Frame = 0

Query: 12  PKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIG 71
           P  + ++   +FR  D +GDGR++ +EL   FR +G  +      R +  +DSD DG+I 
Sbjct: 49  PPAAEDETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYIS 108

Query: 72  EKDF 76
             +F
Sbjct: 109 LGEF 112

BLAST of Spg030266 vs. ExPASy Swiss-Prot
Match: Q84V36 (Polcalcin Che a 3 OS=Chenopodium album OX=3559 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.3e-04
Identity = 21/54 (38.89%), Postives = 35/54 (64.81%), Query Frame = 0

Query: 22 IFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDF 76
          IF+  D +GDG++++SEL +A + +G++ P    RR +   D+D DGFI   +F
Sbjct: 16 IFKRFDTNGDGKISSSELGDALKTLGSVTPD-EVRRMMAEIDTDGDGFISFDEF 68

BLAST of Spg030266 vs. ExPASy Swiss-Prot
Match: P58171 (Polcalcin Syr v 3 OS=Syringa vulgaris OX=34270 GN=SYRV3 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.3e-04
Identity = 21/59 (35.59%), Postives = 38/59 (64.41%), Query Frame = 0

Query: 18 QLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDFE 77
          +L  IF+  D +GDG++++SEL E  + +G++ P    +R +   D+D DGFI  ++F+
Sbjct: 7  ELERIFKRFDANGDGKISSSELGETLKTLGSVTPE-EIQRMMAEIDTDGDGFISFEEFK 64

BLAST of Spg030266 vs. ExPASy TrEMBL
Match: A0A0A0KZR6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-30
Identity = 67/90 (74.44%), Postives = 76/90 (84.44%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SK Y    KLSREQL LIFRNHD D DGRL+T+ELTEAFRYIGA LP+YRAR ALL
Sbjct: 1  MSRKSKNYYPRAKLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPTYRARHALL 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          H+DSDRDGFIGEKDFE L+NYA +R+YT++
Sbjct: 61 HADSDRDGFIGEKDFESLVNYAEQRQYTII 90

BLAST of Spg030266 vs. ExPASy TrEMBL
Match: A0A5A7VDB9 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003480 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.2e-30
Identity = 67/90 (74.44%), Postives = 75/90 (83.33%), Query Frame = 0

Query: 1  MPRNSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALL 60
          M R SKTY    +LSREQL LIFRNHD D DGRL+T+ELTEAFRYIGA LP YRAR AL 
Sbjct: 1  MSRRSKTYHPRARLSREQLLLIFRNHDSDKDGRLSTTELTEAFRYIGAFLPPYRARHALF 60

Query: 61 HSDSDRDGFIGEKDFELLINYAHKRKYTVM 91
          H+DSDRDGFI EKDFELL+NYA +R+YTV+
Sbjct: 61 HADSDRDGFISEKDFELLVNYAEQRQYTVI 90

BLAST of Spg030266 vs. ExPASy TrEMBL
Match: A5AWP8 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-14
Identity = 41/84 (48.81%), Postives = 58/84 (69.05%), Query Frame = 0

Query: 6  KTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSD 65
          KT      L  +QL  IF+ HD +GDG+LN +EL +AF+++GAL+P +RA RAL H+D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLINYAHKRKYTV 90
           DG I EK+ + L+ YA K  YT+
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGYTI 90

BLAST of Spg030266 vs. ExPASy TrEMBL
Match: A0A5N6RQV2 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.2e-13
Identity = 39/75 (52.00%), Postives = 56/75 (74.67%), Query Frame = 0

Query: 15 SREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKD 74
          +R+QL  IFR+HD DGDG+L+  E+  AF Y+G+   SYRA  ALLH+DSD DG++GE++
Sbjct: 16 TRDQLMNIFRSHDLDGDGKLSRKEVKAAFGYLGSRWDSYRAVLALLHADSDHDGYVGEEE 75

Query: 75 FELLINYAHKRKYTV 90
           + L++YA K  YT+
Sbjct: 76 LKKLVDYALKCGYTI 90

BLAST of Spg030266 vs. ExPASy TrEMBL
Match: A0A438DYH7 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_081444 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.6e-13
Identity = 40/82 (48.78%), Postives = 56/82 (68.29%), Query Frame = 0

Query: 6  KTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSD 65
          KT      L  +QL  IF+ HD +GDG+LN +EL +AF+++GAL+P +RA RAL H+D+D
Sbjct: 7  KTIPSKSPLPEQQLKKIFKQHDTNGDGQLNKAELKQAFQHLGALIPGWRAHRALHHADTD 66

Query: 66 RDGFIGEKDFELLINYAHKRKY 88
           DG I EK+ + L+ YA K  Y
Sbjct: 67 GDGCISEKEMKDLVEYAAKFGY 88

BLAST of Spg030266 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-06
Identity = 25/72 (34.72%), Postives = 41/72 (56.94%), Query Frame = 0

Query: 4   NSKTYQQMPKLSREQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSD 63
           N K++Q     S+E+L  +F + D DGDG+++  EL   F  +G  +    A+ A+   D
Sbjct: 54  NIKSHQ----ASKEELRQVFSHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVD 113

Query: 64  SDRDGFIGEKDF 76
           +D DG +G +DF
Sbjct: 114 TDADGSLGFEDF 121

BLAST of Spg030266 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 45.1 bits (105), Expect = 3.6e-05
Identity = 21/62 (33.87%), Postives = 32/62 (51.61%), Query Frame = 0

Query: 17 EQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDFE 76
          E+L  +F   D +GDG+++  EL   F+ +G         R L   D+DRDG+I   +F 
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 77 LL 79
           L
Sbjct: 82 TL 83

BLAST of Spg030266 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 45.1 bits (105), Expect = 3.6e-05
Identity = 21/62 (33.87%), Postives = 32/62 (51.61%), Query Frame = 0

Query: 17 EQLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDFE 76
          E+L  +F   D +GDG+++  EL   F+ +G         R L   D+DRDG+I   +F 
Sbjct: 22 EELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFS 81

Query: 77 LL 79
           L
Sbjct: 82 TL 83

BLAST of Spg030266 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 45.1 bits (105), Expect = 3.6e-05
Identity = 18/58 (31.03%), Postives = 35/58 (60.34%), Query Frame = 0

Query: 18 QLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDF 76
          +L+ +F+  D DGDG++ T EL E+F+ +G ++P     + +   D + DG +  ++F
Sbjct: 5  ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF 62

BLAST of Spg030266 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 43.5 bits (101), Expect = 1.0e-04
Identity = 20/58 (34.48%), Postives = 31/58 (53.45%), Query Frame = 0

Query: 18 QLSLIFRNHDDDGDGRLNTSELTEAFRYIGALLPSYRARRALLHSDSDRDGFIGEKDF 76
          +L  +F   D +GDG+++ SEL   F+ +G         R L   D D DGFI +++F
Sbjct: 20 ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEF 77

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024248.13.8e-3381.11Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6591370.11.4e-3281.82Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia][more]
KGN55185.15.1e-3074.44hypothetical protein Csa_012915 [Cucumis sativus][more]
KAA0064376.18.7e-3074.44putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK20211.1 pu... [more]
KAG6600744.11.9e-2972.22Polcalcin Bet v 4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q0DJV65.4e-0639.66Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q8L3R22.0e-0534.72Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ... [more]
Q6L4D45.9e-0532.81Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q84V361.3e-0438.89Polcalcin Che a 3 OS=Chenopodium album OX=3559 PE=1 SV=1[more]
P581711.3e-0435.59Polcalcin Syr v 3 OS=Syringa vulgaris OX=34270 GN=SYRV3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZR62.5e-3074.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639730 PE=4 SV=1[more]
A0A5A7VDB94.2e-3074.44Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A5AWP81.5e-1448.81Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_023174 PE=4 SV=1[more]
A0A5N6RQV21.2e-1352.00Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_018544 PE=4 SV=1[more]
A0A438DYH71.6e-1348.78Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_081444 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G50770.11.4e-0634.72calmodulin-like 41 [more]
AT1G18210.13.6e-0533.87Calcium-binding EF-hand family protein [more]
AT1G18210.23.6e-0533.87Calcium-binding EF-hand family protein [more]
AT4G03290.13.6e-0531.03EF hand calcium-binding protein family [more]
AT1G73630.11.0e-0434.48EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 2..89
e-value: 3.4E-10
score: 41.7
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 63..75
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 27..39
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 14..49
score: 11.75176
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 8..79

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030266.1Spg030266.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding