Spg029223 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg029223
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold12: 36214556 .. 36219281 (-)
RNA-Seq ExpressionSpg029223
SyntenySpg029223
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGTGAAATATGGGGATTGTAAGAACATTGTTCGAAGCGTGTGGCAGGAATGTGGTCATGGAAGGAATATCTCAATTATGGATAAAACTAGGACTTGTCTGGAGCGCCTAGGAAACTGGAGCAGATATAGGTACGGAGGTTCTATGAGAGGTGCGATATCCAAAAAGGAAAGGGAGATCCAGAGGCTTGCAGGAAGCAATGAGGTGGACAGGTTGCAGTTGATTCTCGAGATGGAAAAAGATTTAGAGGTGTTATTAGAAGATGATGAAATTTACTGGCGTCAAAGCGAATGTGAGGAGTGGTTGCAATGAGGAGATCGAAACACGAAATGATTCCATATGAGGGCAAATCACAGAAGGAAAGTTAATAACATCAAAGGTTTGTTTGACGAAAGAAATCAGTGGTCAGAAGATGAATCTCAGATGGAATCCATAGCAAATCACTACTTCCAAAATCTCTTCAGAACATCTACCCCTGACAGAAATGCTATAGATCGTGTTATGGATTGTATCCCATCTAGTATCACTGGGGAGCAGAATGCTGAACTTATTAAAAAATTCACTAAAGAAGAGATCACGACAGTAGTGAAGAGTATGGCTCCTACGAAAGCTCCTGGTCCAGAAGGTATTCAGGCAATTTTTTACCAAAAATATTGGGATATAGTAGGCGAGGACACTTGTAAATTCTGTTGGCAGATTCTAAATGAAGGGGCTTCTATAAGTCAGATCAATAGAACCTATATTGCTTTGATTCCAAAAGTTAAAGACCCTAAGGCCATGAAGGATTTCAGACCTATTAGCCTTTGCTCAGTGCTGTATAAAATCATCGCCAAGACACTGGCAAATAGGTTGAAAAAAGTCCTTGATAAGATTATATCTCCTAACCAATCAACTTTTGTTCCTGGAAGACTTATCACAGTGATAGCGAGCATAGCTAGCTACTTTTACTTATTTAATTTAGCTAGTCTTTTATGATTTTTGGGCCATTTTATTGTGCTTTATTGTTTATTTTGTAGGAAAGCGATGATTAGAGCTAAAACGTGGAATTGGAGTTAATCGGGTGTTCGGGACGTGAAAATATGCCAAAGAACCGGAAAGTTCAAGCAACAAGGAGCCAAGAGAGGGCATTCTGCCCATCAGCGTCGAGACGCCACTCTTGGAGGGTCTCGACGCTGGTTTTCCTTAATTAGAAGAGGCGGCAGCGCGACGCTGTGACAATAGCGTCTCGACGCTACCGTTATTTCCATTCCAGACGCGCGTGTTTTAGAGGCAGCGTCGAGACGCTGTCTGGATAGCGTCTCGACGCTACGACGAAAAATCAGTATTTATAGTTCTTTTCGGCTAGGTTTTTGGGGGAACTCTTTTGGGATACTTTTGGAGCCGTAAAACAGAGGAGAAAACAGAGGATTGAGGCTGAACAAGAGGGAGAAAAGCGGAAATCAACCCATTGTTCGTGGGGATCGTGACGGGGACGTTCGGTCTCGGCCTACATTGAGTTTTCTTTTCCCTTTCCTTTTCTTAGCGAGATTTTATGGCTTTATTATGTTTAGTTGAGCTTGTAATACAAATCATGAGTAGCTAAACTTGTAGCTTAGGGATTGATGTAGTTGATTGAGTTTATGGATTGATTTATGGTTTAAGTTGTGCAATGTTTATTATGTTTGTAATCTTGATTTAATGCTTTCAATTGATTGGCCATTAGTTGGATGATTTATGTTTTGAATCTAGCTCGGGAGAGGGGATTTGATATAGACATACTCAAGATTGCATGACTAAAGTTTATTTTCTAATTCGTGACGGTTTTAGATTAACTTTAGAATTGGTCAAAAGATCTCATTGCCTTGCATATTAATAAACTTAGATAGGGATATCAAGTTGGTTAATTTGCATGAGAATCAATTATACTCGGGAGAGGTAGTTGTGATTAAAGGGTCTTTGGCTCATAATTTCATTGTGCATAATAACTTACTCCTTAAAATCAATCCTAGACCAAATCGGCGAAATCAATTCCCTAAGTGTTTTTCCAATTGAATTTCCACTTTTTACTTGCTTTGATTTAGTTTTCATATCATTCACTCACTACTTTTTGATCCCTAAATAAAATTGAGCAAGATAATTCACAATACTTGATTTGCTTAAAAATCCAATCCAGAGGACGACACTCGTTTATACGAACACTTTATTATTACTTGACCCGTGCGCTTGCGGTAAGGAAAATTCCACATCACACAGACAATGCCATTTTGGGGTTTGAATGTATCCATGCGATCAAGAGTAAAAGATTGGGAAAGGTGGGAAACTGGACATGAGCAAAGCTTATGATAGGGTCGAATGGGTTTATATCCGGAGGATTATGGGCAAGATGGGATTCAGTAATACCTGGATAAATCTGATAATGGGATGTATTGAATCTGTGAGTTTTCAAGTGCTGCTGAATTGTACACCTCTCTCTACTTTCCATCCGACCAGGGGGATACATCAAGGCGACCCTTTATCTCCATATCTTTTTCTTATTTGTGCAGAAGGCCTTTCGGGTCTTTTAAATGCAGCAGAAAGAGATAAGGACATCACAGGTTTGAGTATCACTAAACACTGCCCTTCTATTTCTCAATTGTTTTATGCTGATGACAGCTTTCTATTTTTCAAAGCTACTAATAAAGATTGCAGGTTAGTGAAAAGAAGCCTAGAGATCTATGAAAAGGCATCGGGTCAAACGGTGAACTTTGAGAAATCTATGTTCAAGTGTGGAGAAGATTCAAACTGTGAAGGATAATCTTGGGGTTTCTCATTCTGGGACATTGGGTCAATATTTAGGTCTCCCTAGCCAGAATGCCAGAAACAAAAAGGAGATCTTCAACCATATTAAAGATAGGGTTTGGAGAACACTTCAAGGATGGAAGGGCAAATTTTTCTCTGCGACAGGAAGGGAAGTTTTAATTAAATCAGTGGCTCAGGCCATCCCTAACTATGCTATGAGTTGCTTAAAGTTTCCGATAAGCCTCTGTTACGATCTTAATTCTTTGTGTGCTAGGTTTTGGTGGGGAGGCTCTGAAGAGGGTAATAAAATCCACTGGCAAAGCTGGAGGAAGCTATGTATTAACAAAGTTCTTGGCGGCATGGGCTTTAAGGACCTCACCATCTTTAATCAAGCAATGCTAGCTAAACAGAGCTAGAGGCTACTCAAATTCCCTAATAGCCTGTTGGCTAGGGTGCTTAGGGGAAGATACTTCAAAACCAAAAATTTCTTACAAGCATCTATGGGCAATAACCCTTCCTATGTTTGGAGGAGTATACTTTGGGGTCGTGAGCTGTTCAAGAAGGGTTTTCGATGGAGGGTTGGTAGTGGTGTTAGTATTGAAGCCTCCTCAGACCCTTGGATTCCAAGGGAGGGATCATGTTATCCGATTGCCCCTCACCAGCATACTCACAATCTCACGGTGGCTCATCTAATCCATACAAGTGGAATTTGGAATGAAAGGCTGATCAGAGACCTTTATATTCAACAGGATGCCAACTTGATTTTGAATATCCCCTGAACCCACAACAAAAGGAGGATGTAATCATATGGAATTACGATCCCAAAGGACAATTCACGGTCAAAAGTGCGTATAGGTTAGGGATGAACATTCAAAATCAACCAATGACCTCTGTTTCCAACGCTAGCCAACAAATTTCCTTATGGAAAAACTACTGGAAAATTCCACTCCCCTCAAAAATCAAGATCTGTGGCTGGCGAATCTACAAGGACATCCTCCCTACTCTTTCTAATTTATATATGAAAGGAACTGATCTAAAAACAGTCTGTCGTCTTTTATGCAGGGAGCGGAAGGAGACATCTTCCCACCTTTTTTGGGGATGTAAAGCCACAAGGGGTATCTGGCCTAAATACTTTCACCCATCTGACTTAATCTGTTTGAATGGCAGGGAAGATTGGGAGCCAAAGGTTTTTCTAGAAAGCATTTGGAGCAGAATGGGATCGGAAGAAGGAGTGATAGAGAAGATGCACAAAAGCTTAATCATTTGCTGGCAGATTTGGAATTACCGAAACCACATACTTCACAACCAGATTCAACCAGATTTTAACACATTGCAGAACCAAATTGCTAACTACATGGAGGAATTGAAAAGATCAAGTGGTCCGAATCAGAGTATACCTCTTCCTGCTACGATCTTATCACGTACGCCAGGTTGGAGTCGTCCTCCAAGTGGGATATGGAAGCTAAACTGTGATGCGGCTTGGTCTGAAGATCTTCAACGTGGAGGAATCGGTTGGATCCCTCGTCAGGCGGACGAAAATCCCATTAGCGCAGGTCACCGGCACATTAACAGACCATGGATAATCTCATGGCTGGAGTCTTTGGCAATTAGCGAGGGTCTTCGGCATCTACCAGAAGACTCGCCTCCCGTCCAAGTTGAAAGCGATGCTCTTCACGTCACACAGCTCCTAAATGGCAGAGAGGAGGATGAATCGAAAGTGCAACTTTTCATTGAGGAAGCAAAATCCCTGATCTCCAAATCTAACATCGAGGGCATTTATCAAGTGAAGAGGATTCACAATCAGATGGCTCACAGCCTGGCTAGAAAGACTTGTTTAAGTGGCAGTTCTGATAGTTGGGCCACTTTATTTCCAAGTTGGTTACTCCAATTAAATTATTCTGACATAGGTGGTTTAAATACCAATAGTGGGGGTCCTGTCCCTCAACCATTGGTTCCTTTACTCTAA

mRNA sequence

ATGGGATCGGAAGAAGGAGTGATAGAGAAGATGCACAAAAGCTTAATCATTTGCTGGCAGATTTGGAATTACCGAAACCACATACTTCACAACCAGATTCAACCAGATTTTAACACATTGCAGAACCAAATTGCTAACTACATGGAGGAATTGAAAAGATCAAGTGGTCCGAATCAGAGTATACCTCTTCCTGCTACGATCTTATCACGTACGCCAGGTTGGAGTCGTCCTCCAAGTGGGATATGGAAGCTAAACTGTGATGCGGCTTGGTCTGAAGATCTTCAACGTGGAGGAATCGGTTGGATCCCTCGTCAGGCGGACGAAAATCCCATTAGCGCAGGTCACCGGCACATTAACAGACCATGGATAATCTCATGGCTGGAGTCTTTGGCAATTAGCGAGGGTCTTCGGCATCTACCAGAAGACTCGCCTCCCGTCCAAGTTGAAAGCGATGCTCTTCACGTCACACAGCTCCTAAATGGCAGAGAGGAGGATGAATCGAAAGTGCAACTTTTCATTGAGGAAGCAAAATCCCTGATCTCCAAATCTAACATCGAGGGCATTTATCAAGTGAAGAGGATTCACAATCAGATGGCTCACAGCCTGGCTAGAAAGACTTGTTTAAGTGGCAGTTCTGATAGTTGGGCCACTTTATTTCCAAGTTGGTTACTCCAATTAAATTATTCTGACATAGGTGGTTTAAATACCAATAGTGGGGGTCCTGTCCCTCAACCATTGGTTCCTTTACTCTAA

Coding sequence (CDS)

ATGGGATCGGAAGAAGGAGTGATAGAGAAGATGCACAAAAGCTTAATCATTTGCTGGCAGATTTGGAATTACCGAAACCACATACTTCACAACCAGATTCAACCAGATTTTAACACATTGCAGAACCAAATTGCTAACTACATGGAGGAATTGAAAAGATCAAGTGGTCCGAATCAGAGTATACCTCTTCCTGCTACGATCTTATCACGTACGCCAGGTTGGAGTCGTCCTCCAAGTGGGATATGGAAGCTAAACTGTGATGCGGCTTGGTCTGAAGATCTTCAACGTGGAGGAATCGGTTGGATCCCTCGTCAGGCGGACGAAAATCCCATTAGCGCAGGTCACCGGCACATTAACAGACCATGGATAATCTCATGGCTGGAGTCTTTGGCAATTAGCGAGGGTCTTCGGCATCTACCAGAAGACTCGCCTCCCGTCCAAGTTGAAAGCGATGCTCTTCACGTCACACAGCTCCTAAATGGCAGAGAGGAGGATGAATCGAAAGTGCAACTTTTCATTGAGGAAGCAAAATCCCTGATCTCCAAATCTAACATCGAGGGCATTTATCAAGTGAAGAGGATTCACAATCAGATGGCTCACAGCCTGGCTAGAAAGACTTGTTTAAGTGGCAGTTCTGATAGTTGGGCCACTTTATTTCCAAGTTGGTTACTCCAATTAAATTATTCTGACATAGGTGGTTTAAATACCAATAGTGGGGGTCCTGTCCCTCAACCATTGGTTCCTTTACTCTAA

Protein sequence

MGSEEGVIEKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYMEELKRSSGPNQSIPLPATILSRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPWIISWLESLAISEGLRHLPEDSPPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLISKSNIEGIYQVKRIHNQMAHSLARKTCLSGSSDSWATLFPSWLLQLNYSDIGGLNTNSGGPVPQPLVPLL
Homology
BLAST of Spg029223 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-26
Identity = 76/217 (35.02%), Postives = 111/217 (51.15%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYMEELKRSSGPNQSIPLPATIL 68
           E +   LI  W IWN+RN+++       F+T+  Q+  ++ E    S    S+ +    L
Sbjct: 8   EDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQS--ETSLSMLHKTL 67

Query: 69  SRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPWIISWLE 128
           +    W  PP  IW LN DA+WS+   RGGIGWI R  D + + AG+R +     +  LE
Sbjct: 68  NNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLE 127

Query: 129 SLAISEGLRHLPEDS--PPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLISKSNIE 188
           + AI EGLR+L       P+ +E+D+  V  LLN + ED +K    +EE  +L     I 
Sbjct: 128 ASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEIL 187

Query: 189 GIYQVKRIHNQMAHSLARKTCLSGSSDSWATLFPSWL 224
              +V R  N  AHSLA++  +   S  W   FP+WL
Sbjct: 188 AFAKVGRETNGCAHSLAQRASVLRESMIWVDCFPNWL 222

BLAST of Spg029223 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 113.6 bits (283), Expect = 2.4e-21
Identity = 71/206 (34.47%), Postives = 104/206 (50.49%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYM-------EELKRSSGPNQSI 68
           E+  +S+II WQIW  RN  +   + P+   +Q  I  Y+         LK  S  N+ +
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKS-TNKDL 66

Query: 69  PLPATILSRTPG-WSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINR 128
            L   I   T   W  P S  WKLN +AAW  D   GGIGWI R      I A  R I  
Sbjct: 67  HLIRRIEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRA 126

Query: 129 PWIISWLESLAISEGLRHL-PEDSPPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSL 188
              I++LE +AI EGLR +  E   P+ +ESD+L    LL+ + +D++++   +EE   +
Sbjct: 127 ERNITYLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQM 186

Query: 189 ISKSNIEGIYQVKRIHNQMAHSLARK 206
           +    I  +  + R  N++AH LAR+
Sbjct: 187 MKDMEIVSMRHISREANKVAHGLARR 211

BLAST of Spg029223 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 109.0 bits (271), Expect = 6.0e-20
Identity = 68/213 (31.92%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYM------EELKRSSGPNQSIP 68
           E+  +S+II WQIW  RN  +   +  +   +Q  I  Y+      +   +    N+ + 
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLH 66

Query: 69  LPATILSRTPG-WSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRP 128
           L   I   T   W  P S  WKLN DAAW  D   GGIGWI R      I A  R I   
Sbjct: 67  LIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTE 126

Query: 129 WIISWLESLAISEGLRHL---------PEDSPPVQVESDALHVTQLLNGREEDESKVQLF 188
             I++LE +AI EGLR +          E   P+ +ESD+L    LL+ + +D++++   
Sbjct: 127 RNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWL 186

Query: 189 IEEAKSLISKSNIEGIYQVKRIHNQMAHSLARK 206
           +EE   ++    I  +  + R  N++AH LAR+
Sbjct: 187 LEEIWQMMEDMKIVSMRHISREANKVAHDLARR 219

BLAST of Spg029223 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 4.8e-17
Identity = 59/200 (29.50%), Postives = 97/200 (48.50%), Query Frame = 0

Query: 10  KMHKSLIICWQIWNYRNHILHNQI-QPDFNTLQNQIANYMEELKRSSGPNQSIPLPATIL 69
           K    L++ W IW YRN I+H+ I QP    ++    + + E   + G N  + +    L
Sbjct: 15  KRQSGLVLLWSIWTYRNQIVHSTIEQPCSQKIRAFTESKIYEFTTNLGGN--LNMEDKNL 74

Query: 70  SRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPWIISWLE 129
            RT GW+ P   +WKLN DA W + L  GG+GWI R ++        R I    + +  +
Sbjct: 75  PRTVGWTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSE-------GRFIMAECLKALSQ 134

Query: 130 SLAISEGLRHLPEDSPPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLISKSNIEGI 189
           SL +  G++        +++ESD L V  ++N      ++V L +E+    +    IEG 
Sbjct: 135 SLTLEAGIK--------IEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGF 194

Query: 190 YQVKRIHNQMAHSLARKTCL 209
             +    N +AH +AR+ C+
Sbjct: 195 KHLPMKANGVAHGIARRACV 197

BLAST of Spg029223 vs. NCBI nr
Match: KAF4360260.1 (hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical protein G4B88_025885 [Cannabis sativa])

HSP 1 Score: 98.6 bits (244), Expect = 8.1e-17
Identity = 64/229 (27.95%), Postives = 105/229 (45.85%), Query Frame = 0

Query: 8   IEKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYMEELKRSSGPNQSIPLPATI 67
           + +   ++ I W IW  RN          +N L       + +   S+ P  S P+   +
Sbjct: 163 MNEFEDAIKIMWAIWENRNR--------KWNNLHVMNGAQLLDWVFSAYPGSSTPVKNDM 222

Query: 68  -----LSRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPW 127
                LS+T  W RPP+G   +NCDAA +      G G+I R  D   + AG  + NR  
Sbjct: 223 TTNMELSKTTHWIRPPAGYIAVNCDAAMNSGAAGIGTGFIWRNWDGKLLIAGMNYSNRCC 282

Query: 128 IISWLESLAISEGLRHLPEDS-PPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLIS 187
            +   E+ AI E L ++P  +  P++++SD  H+   +  ++   + V   I + +  + 
Sbjct: 283 SVDMAEAWAILEALNNMPNTTDSPMEIQSDCKHLVDKIMNQDNHLTAVNTIIHQIRMQME 342

Query: 188 KSNIEGIYQVKRIHNQMAHSLARKTCLSGSSDSWATLFPSWLLQLNYSD 231
           K N   I  V R +N+ AH LARK   + ++  +   FP WL Q   +D
Sbjct: 343 KLNCNSIVHVNRNNNECAHMLARKCLATKTTQIFTHPFPRWLAQFCTAD 383

BLAST of Spg029223 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 5.5e-27
Identity = 76/217 (35.02%), Postives = 111/217 (51.15%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYMEELKRSSGPNQSIPLPATIL 68
           E +   LI  W IWN+RN+++       F+T+  Q+  ++ E    S    S+ +    L
Sbjct: 8   EDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTESSYQS--ETSLSMLHKTL 67

Query: 69  SRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPWIISWLE 128
           +    W  PP  IW LN DA+WS+   RGGIGWI R  D + + AG+R +     +  LE
Sbjct: 68  NNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLE 127

Query: 129 SLAISEGLRHLPEDS--PPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLISKSNIE 188
           + AI EGLR+L       P+ +E+D+  V  LLN + ED +K    +EE  +L     I 
Sbjct: 128 ASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEIL 187

Query: 189 GIYQVKRIHNQMAHSLARKTCLSGSSDSWATLFPSWL 224
              +V R  N  AHSLA++  +   S  W   FP+WL
Sbjct: 188 AFAKVGRETNGCAHSLAQRASVLRESMIWVDCFPNWL 222

BLAST of Spg029223 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.2e-21
Identity = 71/206 (34.47%), Postives = 104/206 (50.49%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYM-------EELKRSSGPNQSI 68
           E+  +S+II WQIW  RN  +   + P+   +Q  I  Y+         LK  S  N+ +
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKS-TNKDL 66

Query: 69  PLPATILSRTPG-WSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINR 128
            L   I   T   W  P S  WKLN +AAW  D   GGIGWI R      I A  R I  
Sbjct: 67  HLIRRIEDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRA 126

Query: 129 PWIISWLESLAISEGLRHL-PEDSPPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSL 188
              I++LE +AI EGLR +  E   P+ +ESD+L    LL+ + +D++++   +EE   +
Sbjct: 127 ERNITYLEVMAICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQM 186

Query: 189 ISKSNIEGIYQVKRIHNQMAHSLARK 206
           +    I  +  + R  N++AH LAR+
Sbjct: 187 MKDMEIVSMRHISREANKVAHGLARR 211

BLAST of Spg029223 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.9e-20
Identity = 68/213 (31.92%), Postives = 102/213 (47.89%), Query Frame = 0

Query: 9   EKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYM------EELKRSSGPNQSIP 68
           E+  +S+II WQIW  RN  +   +  +   +Q  I  Y+      +   +    N+ + 
Sbjct: 7   EERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDLH 66

Query: 69  LPATILSRTPG-WSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRP 128
           L   I   T   W  P S  WKLN DAAW  D   GGIGWI R      I A  R I   
Sbjct: 67  LIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTE 126

Query: 129 WIISWLESLAISEGLRHL---------PEDSPPVQVESDALHVTQLLNGREEDESKVQLF 188
             I++LE +AI EGLR +          E   P+ +ESD+L    LL+ + +D++++   
Sbjct: 127 RNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWL 186

Query: 189 IEEAKSLISKSNIEGIYQVKRIHNQMAHSLARK 206
           +EE   ++    I  +  + R  N++AH LAR+
Sbjct: 187 LEEIWQMMEDMKIVSMRHISREANKVAHDLARR 219

BLAST of Spg029223 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 2.3e-17
Identity = 59/200 (29.50%), Postives = 97/200 (48.50%), Query Frame = 0

Query: 10  KMHKSLIICWQIWNYRNHILHNQI-QPDFNTLQNQIANYMEELKRSSGPNQSIPLPATIL 69
           K    L++ W IW YRN I+H+ I QP    ++    + + E   + G N  + +    L
Sbjct: 15  KRQSGLVLLWSIWTYRNQIVHSTIEQPCSQKIRAFTESKIYEFTTNLGGN--LNMEDKNL 74

Query: 70  SRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPWIISWLE 129
            RT GW+ P   +WKLN DA W + L  GG+GWI R ++        R I    + +  +
Sbjct: 75  PRTVGWTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSE-------GRFIMAECLKALSQ 134

Query: 130 SLAISEGLRHLPEDSPPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLISKSNIEGI 189
           SL +  G++        +++ESD L V  ++N      ++V L +E+    +    IEG 
Sbjct: 135 SLTLEAGIK--------IEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGF 194

Query: 190 YQVKRIHNQMAHSLARKTCL 209
             +    N +AH +AR+ C+
Sbjct: 195 KHLPMKANGVAHGIARRACV 197

BLAST of Spg029223 vs. ExPASy TrEMBL
Match: A0A7J6ERF5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.9e-17
Identity = 64/229 (27.95%), Postives = 105/229 (45.85%), Query Frame = 0

Query: 8   IEKMHKSLIICWQIWNYRNHILHNQIQPDFNTLQNQIANYMEELKRSSGPNQSIPLPATI 67
           + +   ++ I W IW  RN          +N L       + +   S+ P  S P+   +
Sbjct: 163 MNEFEDAIKIMWAIWENRNR--------KWNNLHVMNGAQLLDWVFSAYPGSSTPVKNDM 222

Query: 68  -----LSRTPGWSRPPSGIWKLNCDAAWSEDLQRGGIGWIPRQADENPISAGHRHINRPW 127
                LS+T  W RPP+G   +NCDAA +      G G+I R  D   + AG  + NR  
Sbjct: 223 TTNMELSKTTHWIRPPAGYIAVNCDAAMNSGAAGIGTGFIWRNWDGKLLIAGMNYSNRCC 282

Query: 128 IISWLESLAISEGLRHLPEDS-PPVQVESDALHVTQLLNGREEDESKVQLFIEEAKSLIS 187
            +   E+ AI E L ++P  +  P++++SD  H+   +  ++   + V   I + +  + 
Sbjct: 283 SVDMAEAWAILEALNNMPNTTDSPMEIQSDCKHLVDKIMNQDNHLTAVNTIIHQIRMQME 342

Query: 188 KSNIEGIYQVKRIHNQMAHSLARKTCLSGSSDSWATLFPSWLLQLNYSD 231
           K N   I  V R +N+ AH LARK   + ++  +   FP WL Q   +D
Sbjct: 343 KLNCNSIVHVNRNNNECAHMLARKCLATKTTQIFTHPFPRWLAQFCTAD 383

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155262.11.1e-2635.02uncharacterized protein LOC111022403 [Momordica charantia][more]
XP_022143535.12.4e-2134.47uncharacterized protein LOC111013412 [Momordica charantia][more]
XP_022156777.16.0e-2031.92uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_022148549.14.8e-1729.50uncharacterized protein LOC111017181 [Momordica charantia][more]
KAF4360260.18.1e-1727.95hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical p... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DNV95.5e-2735.02uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1CP261.2e-2134.47uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1DSV12.9e-2031.92uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1D4B62.3e-1729.50uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A7J6ERF53.9e-1727.95Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 85..205
e-value: 4.4E-14
score: 52.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 82..211
e-value: 9.2E-10
score: 40.6
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 71..223
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 71..223
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 84..204
e-value: 3.28949E-16
score: 70.806
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 81..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg029223.1Spg029223.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity