Spg028914 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg028914
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAminoacyl-tRNA hydrolase
Locationscaffold5: 14240640 .. 14242039 (+)
RNA-Seq ExpressionSpg028914
SyntenySpg028914
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTCATGTGCTACTTTGTGTGAAGATATCATGACATCTGTAATTTCAGCATCTCGATTCACACTCTCCATTCCCAATTCCACCAGGCTCTGTTCAAGGGCTAGGGTTTGGTCATCTTCTTCACTCAGTCTTCGGTCTACGAATCGAAATTCCAATCCATTTAGCTCAGCTTCAATGAGTGAATCCACTGAAAGTAATTCCATTCTTCCATTGGACGACGGCGGAGCGAAGGCGGAGGAAACCGTCGATGTTTTGGTTCAATACGTGGTGCTCCGGAGAGATTTGATCGATTCGTGGCCGCTCGGAAGTGTCGTAACTCAGGGATGCCATGCTTCAGTTTCCGCCATTTGGTTGAACAAAGACGATCCCCACACTTCAGAGTACTGTAGTCCCGAAAACATCGATTCTATGCACAAGGTAAGTCGAAGTTTGAATTCCATTGTTACTTTTTATTTTCGAGCGAAGTGCCTCGAACTCGAACAATGTAGTGTGCATCTGTAGCCTTGATCCTTTTTTGTTGATATGCTGAAAATTTGCTTTGTTGGTGCTCTATCAAGTCGGTCTAACAGTTGACAAATGAAACATTTTTTTAGCAATTTGGAACTTCCGTTTTGTGGTTGTGGTCACTGGTTGATTTATTTAATTTTGGGAAGAACATTTTGTCTGTCGAGCTAATCTTCATCGTTCTTCCGTTGAGTTCTAGCGTTTTGGAAGAACAATGGTTGATTGGAAGTCTATTTTTTATTTCGGTTGTTGTTTTCCTTGTGTATATTTTTGTTATTCGGAAGTTTGAATACTCCTTGCTCGTGTTAAGCTAAATCTTTTGAATTTTCCTCCTAATAATATCATATGGTTGTATCTATTTACAATCTCTGCTATAATCGGATGTCTAAGAGAGCTCATTACTTTCAAAACTCTGCCAATACATCTTCTTTTATTTTTATGTAGCTTTCAAGCATTTTTCATGGCTATCAACCATTTCACATCGTTGCCTGTATACTAGCTCCATGGTTAATGCAAACCCACTTCATTTCGCACTTGCAAAACGATTGAGCTGCCAATTGGAATTTTCATATTATTATCTTGTGCAACTTAATTTATTAAAATTACAGGTGTTCTATATTTCATAGAACACGTCTTTTGTCGTTCTGGACTTTTTGATCCCTGAACTGGGTATTATAATACTGACTTTATTTCACCTTTTCTTTATTTAGGTTACTCTCGAGGTGAAGGGAGAAACCCAGATCATTAACTTATCAGAAAAACTTAAAGCAAATTCAATCGTCCATAAACTCTGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCCACAAAGCCCTACCCCAAATCTGTTGTATCAACATTTTTTAAAAAGCTGAAACTCTCTAAGTGA

mRNA sequence

ATGGTTTCATGTGCTACTTTGTGTGAAGATATCATGACATCTGTAATTTCAGCATCTCGATTCACACTCTCCATTCCCAATTCCACCAGGCTCTGTTCAAGGGCTAGGGTTTGGTCATCTTCTTCACTCAGTCTTCGGTCTACGAATCGAAATTCCAATCCATTTAGCTCAGCTTCAATGAGTGAATCCACTGAAAGTAATTCCATTCTTCCATTGGACGACGGCGGAGCGAAGGCGGAGGAAACCGTCGATGTTTTGGTTCAATACGTGGTGCTCCGGAGAGATTTGATCGATTCGTGGCCGCTCGGAAGTGTCGTAACTCAGGGATGCCATGCTTCAGTTTCCGCCATTTGGTTGAACAAAGACGATCCCCACACTTCAGAGTACTGTAGTCCCGAAAACATCGATTCTATGCACAAGGTTACTCTCGAGGTGAAGGGAGAAACCCAGATCATTAACTTATCAGAAAAACTTAAAGCAAATTCAATCGTCCATAAACTCTGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCCACAAAGCCCTACCCCAAATCTGTTGTATCAACATTTTTTAAAAAGCTGAAACTCTCTAAGTGA

Coding sequence (CDS)

ATGGTTTCATGTGCTACTTTGTGTGAAGATATCATGACATCTGTAATTTCAGCATCTCGATTCACACTCTCCATTCCCAATTCCACCAGGCTCTGTTCAAGGGCTAGGGTTTGGTCATCTTCTTCACTCAGTCTTCGGTCTACGAATCGAAATTCCAATCCATTTAGCTCAGCTTCAATGAGTGAATCCACTGAAAGTAATTCCATTCTTCCATTGGACGACGGCGGAGCGAAGGCGGAGGAAACCGTCGATGTTTTGGTTCAATACGTGGTGCTCCGGAGAGATTTGATCGATTCGTGGCCGCTCGGAAGTGTCGTAACTCAGGGATGCCATGCTTCAGTTTCCGCCATTTGGTTGAACAAAGACGATCCCCACACTTCAGAGTACTGTAGTCCCGAAAACATCGATTCTATGCACAAGGTTACTCTCGAGGTGAAGGGAGAAACCCAGATCATTAACTTATCAGAAAAACTTAAAGCAAATTCAATCGTCCATAAACTCTGGATAGAACAACCAGAGAACATTCCAACATGTCTTGCCACAAAGCCCTACCCCAAATCTGTTGTATCAACATTTTTTAAAAAGCTGAAACTCTCTAAGTGA

Protein sequence

MVSCATLCEDIMTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILPLDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVSTFFKKLKLSK
Homology
BLAST of Spg028914 vs. NCBI nr
Match: XP_023546189.1 (putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.7 bits (831), Expect = 5.6e-85
Identity = 172/202 (85.15%), Postives = 179/202 (88.61%), Query Frame = 0

Query: 1   MVSCATLCEDIMTSVISASRFTLSIPNSTRL--CSRARVWSSSSLSLRSTNRNSNPFSSA 60
           MVSCATLCEDIM SVISAS FT SI  ST+L  CSR+RVW SSSLSLRS NRNSN FSSA
Sbjct: 1   MVSCATLCEDIMISVISASPFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSA 60

Query: 61  SMSESTESNSILPLDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIW 120
           SMSES ESNS  P DDG    E+T DVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+W
Sbjct: 61  SMSESAESNSAPPPDDG---VEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVW 120

Query: 121 LNKDDPHTSEYCSPENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTC 180
           LNKDDPHTSEYCSP NIDSMHKVTLEVKGETQI+NLSEKLKA+SI HKLWIEQPENIPTC
Sbjct: 121 LNKDDPHTSEYCSPHNIDSMHKVTLEVKGETQIVNLSEKLKASSIAHKLWIEQPENIPTC 180

Query: 181 LATKPYPKSVVSTFFKKLKLSK 201
           LATKPYPKSVVS FFKKLKL K
Sbjct: 181 LATKPYPKSVVSAFFKKLKLCK 199

BLAST of Spg028914 vs. NCBI nr
Match: XP_023002512.1 (putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 320.1 bits (819), Expect = 1.4e-83
Identity = 165/189 (87.30%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLCS ARVW  SS+ L S N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKLSK 201
           FFKKLKL K
Sbjct: 181 FFKKLKLCK 189

BLAST of Spg028914 vs. NCBI nr
Match: XP_022951520.1 (putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 318.9 bits (816), Expect = 3.1e-83
Identity = 165/189 (87.30%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLC  ARVW  SS+ LRS N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSLRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKLSK 201
           FFKKLKL K
Sbjct: 181 FFKKLKLCK 189

BLAST of Spg028914 vs. NCBI nr
Match: XP_023002513.1 (putative peptidyl-tRNA hydrolase PTRHD1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 318.9 bits (816), Expect = 3.1e-83
Identity = 164/187 (87.70%), Postives = 170/187 (90.91%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLCS ARVW  SS+ L S N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKL 199
           FFKKLKL
Sbjct: 181 FFKKLKL 187

BLAST of Spg028914 vs. NCBI nr
Match: KAG6585426.1 (putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.2 bits (814), Expect = 5.2e-83
Identity = 164/188 (87.23%), Postives = 172/188 (91.49%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS+RLC  ARVW  SS+ LRS N NS  FSSASMSES ES+S+LP
Sbjct: 1   MASVISASRFTFSISNSSRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESSSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKLS 200
           FFKKLKLS
Sbjct: 181 FFKKLKLS 188

BLAST of Spg028914 vs. ExPASy Swiss-Prot
Match: Q3SZ85 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 8.5e-28
Identity = 61/119 (51.26%), Postives = 82/119 (68.91%), Query Frame = 0

Query: 85  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPENIDSMHKV 144
           +LVQY+VLR+DL     SWP G+VV Q CHA+ +A+ L++D PHT+ Y     ++ M KV
Sbjct: 24  ILVQYLVLRKDLTQPPFSWPAGAVVAQACHAATAALHLHRDHPHTAAYL--RELERMRKV 83

Query: 145 TLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVSTFFKKLKLSK 201
            LE   ET +  L+E L+  +I HKLW+EQPENIPTC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAPDETTLKLLAETLQQKNIDHKLWMEQPENIPTCIALRPYPKEEVSQYLKKFRLFK 140

BLAST of Spg028914 vs. ExPASy Swiss-Prot
Match: D3Z4S3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.9e-26
Identity = 58/119 (48.74%), Postives = 79/119 (66.39%), Query Frame = 0

Query: 85  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPENIDSMHKV 144
           +LVQY+VLR+DL     SWP G++V Q CHA+ +A+ L++D PHT+ Y     +  M KV
Sbjct: 24  ILVQYLVLRKDLSQAPFSWPTGALVAQACHAATAALHLHRDHPHTAAYL--RELGRMRKV 83

Query: 145 TLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVSTFFKKLKLSK 201
            LE   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  VS + KK +L K
Sbjct: 84  VLEAADETTLKELAETLQQKNIDHTLWLEQPENIATCIALRPYPKEEVSQYLKKFRLFK 140

BLAST of Spg028914 vs. ExPASy Swiss-Prot
Match: Q6GMV3 (Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.9e-25
Identity = 57/119 (47.90%), Postives = 78/119 (65.55%), Query Frame = 0

Query: 85  VLVQYVVLRRDLID---SWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCSPENIDSMHKV 144
           VLVQY+VLR+DL     SWP G++V Q CHA+ +A+  ++D PHT+ Y   + +  M KV
Sbjct: 24  VLVQYLVLRKDLSQAPFSWPAGALVAQACHAATAALHTHRDHPHTAAYL--QELGRMRKV 83

Query: 145 TLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVSTFFKKLKLSK 201
            LE   ET +  L+E L+  +I H LW+EQPENI TC+A +PYPK  V  + KK +L K
Sbjct: 84  VLEAPDETTLKELAETLQQKNIDHMLWLEQPENIATCIALRPYPKEEVGQYLKKFRLFK 140

BLAST of Spg028914 vs. ExPASy TrEMBL
Match: A0A6J1KJQ6 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 6.6e-84
Identity = 165/189 (87.30%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLCS ARVW  SS+ L S N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKLSK 201
           FFKKLKL K
Sbjct: 181 FFKKLKLCK 189

BLAST of Spg028914 vs. ExPASy TrEMBL
Match: A0A6J1KTS0 (Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.5e-83
Identity = 164/187 (87.70%), Postives = 170/187 (90.91%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLCS ARVW  SS+ L S N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSARLCSNARVWLPSSICLLSWNWNSISFSSASMSESAESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKL 199
           FFKKLKL
Sbjct: 181 FFKKLKL 187

BLAST of Spg028914 vs. ExPASy TrEMBL
Match: A0A6J1GJ11 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.5e-83
Identity = 165/189 (87.30%), Postives = 171/189 (90.48%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLC  ARVW  SS+ LRS N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSLRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKLSK 201
           FFKKLKL K
Sbjct: 181 FFKKLKLCK 189

BLAST of Spg028914 vs. ExPASy TrEMBL
Match: A0A6J1GHT5 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.3e-83
Identity = 164/187 (87.70%), Postives = 170/187 (90.91%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRLCSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSILP 71
           M SVISASRFT SI NS RLC  ARVW  SS+ LRS N NS  FSSASMSES ESNS+LP
Sbjct: 1   MASVISASRFTFSISNSLRLCWNARVWLPSSICLRSWNWNSISFSSASMSESGESNSVLP 60

Query: 72  LDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 131
           LDDGGAKAEE  DVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS
Sbjct: 61  LDDGGAKAEEAADVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEYCS 120

Query: 132 PENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVVST 191
           P+NIDSMHKVTLEVKGETQI+NLSEKLKANSIVHKLW+EQPENIPTCLA+KPYPKSVVS 
Sbjct: 121 PQNIDSMHKVTLEVKGETQIVNLSEKLKANSIVHKLWVEQPENIPTCLASKPYPKSVVSP 180

Query: 192 FFKKLKL 199
           FFKKLKL
Sbjct: 181 FFKKLKL 187

BLAST of Spg028914 vs. ExPASy TrEMBL
Match: A0A6J1HBR1 (Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 9.3e-78
Identity = 159/191 (83.25%), Postives = 166/191 (86.91%), Query Frame = 0

Query: 12  MTSVISASRFTLSIPNSTRL--CSRARVWSSSSLSLRSTNRNSNPFSSASMSESTESNSI 71
           M SVISASRFT SI  ST+L  CSR+RVW SSSLSLRS NRNSN FSS SMSES ESNS 
Sbjct: 1   MISVISASRFTFSISKSTKLCSCSRSRVWPSSSLSLRSRNRNSNLFSSDSMSESAESNSA 60

Query: 72  LPLDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASVSAIWLNKDDPHTSEY 131
            P DD     E+T DVL+QYVVLRRDLIDSWPLGSVVTQGCHASVSA+WLNKDDPHT EY
Sbjct: 61  PPPDD---SVEKTADVLIQYVVLRRDLIDSWPLGSVVTQGCHASVSAVWLNKDDPHTFEY 120

Query: 132 CSPENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPENIPTCLATKPYPKSVV 191
           CSP NIDSMHKVTLEVKGETQI+NLSEKL+ANSI HKLWIEQPENIPTCLATKPYPKSVV
Sbjct: 121 CSPHNIDSMHKVTLEVKGETQIVNLSEKLRANSIAHKLWIEQPENIPTCLATKPYPKSVV 180

Query: 192 STFFKKLKLSK 201
           S FFKKLKL K
Sbjct: 181 SAFFKKLKLCK 188

BLAST of Spg028914 vs. TAIR 10
Match: AT5G10700.1 (Peptidyl-tRNA hydrolase II (PTH2) family protein )

HSP 1 Score: 207.6 bits (527), Expect = 9.2e-54
Identity = 105/146 (71.92%), Postives = 122/146 (83.56%), Query Frame = 0

Query: 55  FSSASMSESTESNSILPLDDGGAKAEETVDVLVQYVVLRRDLIDSWPLGSVVTQGCHASV 114
           F++AS S S ++  I  + D  + AE+  DV+VQYVVLRRDLIDSWPLGSVVTQGCHASV
Sbjct: 41  FTTASPSMSQQTGEIDAVVD-ASSAEKPDDVVVQYVVLRRDLIDSWPLGSVVTQGCHASV 100

Query: 115 SAIWLNKDDPHTSEYCSPENIDSMHKVTLEVKGETQIINLSEKLKANSIVHKLWIEQPEN 174
           +AIW  KDDP T +YC P++IDSMHKVTLEVKGETQ++NLSEKLK   I HKLW+EQPEN
Sbjct: 101 AAIWSFKDDPVTLQYCDPQHIDSMHKVTLEVKGETQMMNLSEKLKLGGISHKLWMEQPEN 160

Query: 175 IPTCLATKPYPKSVVSTFFKKLKLSK 201
           IPTC+ATKPYPKS VS+FFKKLKL K
Sbjct: 161 IPTCIATKPYPKSQVSSFFKKLKLCK 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546189.15.6e-8585.15putative peptidyl-tRNA hydrolase PTRHD1 [Cucurbita pepo subsp. pepo][more]
XP_023002512.11.4e-8387.30putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita maxima][more]
XP_022951520.13.1e-8387.30putative peptidyl-tRNA hydrolase PTRHD1 isoform X1 [Cucurbita moschata][more]
XP_023002513.13.1e-8387.70putative peptidyl-tRNA hydrolase PTRHD1 isoform X2 [Cucurbita maxima][more]
KAG6585426.15.2e-8387.23putative peptidyl-tRNA hydrolase PTRHD1, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
Q3SZ858.5e-2851.26Putative peptidyl-tRNA hydrolase PTRHD1 OS=Bos taurus OX=9913 GN=PTRHD1 PE=2 SV=... [more]
D3Z4S37.9e-2648.74Putative peptidyl-tRNA hydrolase PTRHD1 OS=Mus musculus OX=10090 GN=Ptrhd1 PE=1 ... [more]
Q6GMV33.9e-2547.90Putative peptidyl-tRNA hydrolase PTRHD1 OS=Homo sapiens OX=9606 GN=PTRHD1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1KJQ66.6e-8487.30Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1[more]
A0A6J1KTS01.5e-8387.70Aminoacyl-tRNA hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111496331 PE=4 SV=1[more]
A0A6J1GJ111.5e-8387.30Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1[more]
A0A6J1GHT53.3e-8387.70Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111454312 PE=4 SV=1[more]
A0A6J1HBR19.3e-7883.25Aminoacyl-tRNA hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111462634 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G10700.19.2e-5471.92Peptidyl-tRNA hydrolase II (PTH2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023476Peptidyl-tRNA hydrolase II domain superfamilyGENE3D3.40.1490.10Bit1coord: 86..198
e-value: 1.3E-17
score: 65.9
IPR023476Peptidyl-tRNA hydrolase II domain superfamilySUPERFAMILY102462Peptidyl-tRNA hydrolase IIcoord: 86..198
IPR002833Peptidyl-tRNA hydrolase, PTH2PFAMPF01981PTH2coord: 87..198
e-value: 6.6E-24
score: 84.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..75
NoneNo IPR availableCDDcd02429PTH2_likecoord: 86..198
e-value: 3.87316E-66
score: 197.241
IPR042237Putative peptidyl-tRNA hydrolase PTRHD1PANTHERPTHR46194PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATEDcoord: 60..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg028914.1Spg028914.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004045 aminoacyl-tRNA hydrolase activity