Spg028816 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg028816 vs. NCBI nr
Match: XP_022941638.1 (SEC14 cytosolic factor-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 440.7 bits (1132), Expect = 8.5e-120
Identity = 219/242 (90.50%), Postives = 229/242 (94.63%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DSRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWRKWRDA+V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+SESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD ATLKNILIVSNEEERR MIEEVGEEVLP EYGGKAKFI LQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. NCBI nr
Match: KAG6599921.1 (hypothetical protein SDJN03_05154, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436.8 bits (1122), Expect = 1.2e-118
Identity = 217/242 (89.67%), Postives = 228/242 (94.21%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M++SRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWRKWRDA+V
Sbjct: 1   MENSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+ ESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD ATLKNILIVSNEEERR MIEEVGEEVLP EYGGKAKFI LQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. NCBI nr
Match: XP_023537304.1 (SEC14 cytosolic factor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434.9 bits (1117), Expect = 4.6e-118
Identity = 216/242 (89.26%), Postives = 226/242 (93.39%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DSRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWRKWRDA+V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+ ESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD  TLKNI IVSNEEERR MIEEVGEEVLP EYGGKAKFI LQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPTTLKNISIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. NCBI nr
Match: XP_022996420.1 (SEC14 cytosolic factor-like [Cucurbita maxima])

HSP 1 Score: 429.9 bits (1104), Expect = 1.5e-116
Identity = 214/242 (88.43%), Postives = 225/242 (92.98%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DSRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWR WRDAMV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+ ESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG +GKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSEGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD ATLKNILIV N+EERR MIEEVGEEVLP EYGGKAKFI LQDAVLP 
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPR 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. NCBI nr
Match: XP_038893024.1 (sec14 cytosolic factor-like [Benincasa hispida])

HSP 1 Score: 422.9 bits (1086), Expect = 1.8e-114
Identity = 206/241 (85.48%), Postives = 224/241 (92.95%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DS  NA+IQ+R S+EKSG STQ   DATMMRFLIARSMD SK +KMFVQWRKWRDAMV
Sbjct: 1   MEDSGTNAMIQLRNSVEKSGFSTQNCSDATMMRFLIARSMDVSKASKMFVQWRKWRDAMV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF++ESEV +EL+A+KIFLQGLS  +LPVMIV+ANRHF +KDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFIAESEVEDELKAKKIFLQGLSISQLPVMIVLANRHFHAKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIA+VDL+QISYKN+DPRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLRQISYKNIDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VS+WRMVSRFVDSATLKNI IVSNEEERRIMIEEVGEEVLP EYGGKAKFI LQD +LPH
Sbjct: 181 VSVWRMVSRFVDSATLKNISIVSNEEERRIMIEEVGEEVLPDEYGGKAKFIALQDVLLPH 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of Spg028816 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.4e-13
Identity = 58/247 (23.48%), Postives = 116/247 (46.96%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWR---- 60
           ++ ++   L  MR  ++K G  T+   DAT++RFL AR  +  +  +MF++  KWR    
Sbjct: 24  LNSTQQATLDSMRLELQKLGY-TERLDDATLLRFLRARKFNLQQSLEMFIKCEKWRKEFG 83

Query: 61  -DAMVPKGFVSESEVME----------ELEARKIFLQGLSKKELPVMIVMANRHFPSKDQ 120
            D ++      E E +           +++ R ++++ L   +L  +  +       +  
Sbjct: 84  VDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGNIDLKKLYQITT----PERM 143

Query: 121 LQFKKFLVHLLDKVIASGCKGKEIG-NEKWIAMVDLQQISYKNVDP--RGLITGFQFLQN 180
           +Q   +   +L       C  K  G  E    ++DL+ +   ++      +       Q+
Sbjct: 144 MQNLVYEYEMLALKRFPACSRKAGGLIETSCTIMDLKGVGITSIHSVYSYIRQASSISQD 203

Query: 181 YYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPV 230
           YYPER+GK +++N PW F S + ++  F+D AT+K I I+ +   +  ++E++  + LP 
Sbjct: 204 YYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGS-NYKSALLEQIPADNLPA 263

BLAST of Spg028816 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 77.4 bits (189), Expect = 2.5e-13
Identity = 65/245 (26.53%), Postives = 119/245 (48.57%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           +D ++  AL ++R+ +E +G   +   D+T++RFL AR  D     +MF    KWR    
Sbjct: 29  LDSAQEKALAELRKLLEDAGF-IERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYG 88

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKE---LPVM---IVMANRHFPSK--DQLQFKKFL 120
               + +    E+    K + Q   K +    PV    +   N H  +K   + +  K L
Sbjct: 89  TDTILQDFHYDEKPLIAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNL 148

Query: 121 VHLLDKVI------ASGCKGKEIGNEKWIAMVDLQQISYKNV-DPRGLITGFQFL-QNYY 180
           V   + V+       S   G  +  E    ++DL+ IS  +       +    ++ QNYY
Sbjct: 149 VWEYESVVQYRLPACSRAAGHLV--ETSCTIMDLKGISISSAYSVMSYVREASYISQNYY 208

Query: 181 PERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEY 230
           PER+GK +I+N P+ F + +R+   F+D  T+  I I+ +  ++  +++++  E LPV++
Sbjct: 209 PERMGKFYIINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQKE-LLKQIPAENLPVKF 268

BLAST of Spg028816 vs. ExPASy Swiss-Prot
Match: P53989 (SEC14 cytosolic factor OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=SEC14 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 6.1e-12
Identity = 61/246 (24.80%), Postives = 117/246 (47.56%), Query Frame = 0

Query: 2   DDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMVP 61
           D+++  AL Q+R  +E +G   +   D+T++RFL AR  D +   +MF    KWR     
Sbjct: 28  DEAQEGALKQLRSELEAAGFK-ERLDDSTLLRFLRARKFDVALAKEMFENCEKWRKEYGT 87

Query: 62  KGFVSESEVMEELEARKIFLQGLSK----------KELPVMIVMANRHFPSKDQLQFKKF 121
              + +    E+    K + Q   K          +EL  + +       +++++   K 
Sbjct: 88  NTIMQDFHYDEKPLVAKYYPQYYHKTDKDGRPVYFEELGAVNLTEMEKITTQERM--LKN 147

Query: 122 LVHLLDKVI------ASGCKGKEIGNEKWIAMVDLQQISYKNV-DPRGLITGFQFL-QNY 181
           LV   + V+       S   G  +  E    ++DL+ IS  +       +    ++ QNY
Sbjct: 148 LVWEYESVVNYRLPACSRAAGYLV--ETSCTVMDLKGISISSAYSVLSYVREASYISQNY 207

Query: 182 YPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVE 230
           YPER+GK +++N P+ F + +R+   F+D  T+  I I+ +  +   +++++  E LP +
Sbjct: 208 YPERMGKFYLINAPFGFSTAFRLFKPFLDPVTVSKIFILGSSYQSE-LLKQIPAENLPSK 267

BLAST of Spg028816 vs. ExPASy Swiss-Prot
Match: Q75DK1 (SEC14 cytosolic factor OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEC14 PE=3 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.3e-11
Identity = 56/242 (23.14%), Postives = 117/242 (48.35%), Query Frame = 0

Query: 8   ALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWR-----DAMVPK 67
           AL ++R+ ++++G  T+   D+T++RFL AR  D +    MF    KWR     D +   
Sbjct: 34  ALEELRKVLKQAGF-TKRLDDSTLLRFLRARKFDVAAARAMFENCEKWRKENGVDTIFED 93

Query: 68  GFVSESEVMEEL----------EARKIFLQGLSKKELPVMIVMANRHFPSKDQL-QFKKF 127
               E  ++ +           + R ++++ L    L  M  +  +    K+ + +++ F
Sbjct: 94  FHYEEKPLVAKFYPQYYHKTDKDGRPVYIEELGAVNLTEMYKITTQERMLKNLIWEYESF 153

Query: 128 LVHLLDKVIASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGF-----QFLQNYYPE 187
             + L     +  +  +   E    ++DL+ IS   +     +  +        QNYYPE
Sbjct: 154 SRYRL----PASSRQADCLVETSCTILDLKGIS---ISAAAQVLSYVREASNIGQNYYPE 213

Query: 188 RLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGG 229
           R+GK +++N P+ F + +R+   F+D  T+  I I+ +  ++  +++++  E LPV++GG
Sbjct: 214 RMGKFYMINAPFGFSAAFRLFKPFLDPVTVSKIFILGSSYQKE-LLKQIPAENLPVKFGG 266

BLAST of Spg028816 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.3e-11
Identity = 62/240 (25.83%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 8   ALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMVPKGFV-- 67
           AL+Q R  + +     +   D+T++RFL AR  D +   +MFV+  +WR+       +  
Sbjct: 32  ALLQFRSILLEKNYK-ERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTIIED 91

Query: 68  ----SESEVMEELEARKIFLQ---GLSKKELPVMI-----VMANRHFPSKDQLQFKKFLV 127
                E+E  E ++  K++ Q    + K   P+       +   + +    + Q  + LV
Sbjct: 92  YENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLV 151

Query: 128 ---HLLDKVIASGCKGKEIGN--EKWIAMVDLQQISYKNVDP--RGLITGFQFLQNYYPE 187
               L        C  +  G   E    ++DL+ IS  N       +       QNYYPE
Sbjct: 152 KEYELFATYRVPAC-SRRAGYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYYPE 211

Query: 188 RLGKCFILNMPWFFVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGG 227
           R+GK +I++ P+ F ++++MV  F+D  T+  I I+ +  ++  +++++  E LPV+YGG
Sbjct: 212 RMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKIFILGSSYKKE-LLKQIPIENLPVKYGG 268

BLAST of Spg028816 vs. ExPASy TrEMBL
Match: A0A6J1FSN7 (SEC14 cytosolic factor-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446938 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 4.1e-120
Identity = 219/242 (90.50%), Postives = 229/242 (94.63%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DSRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWRKWRDA+V
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRKWRDAVV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+SESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFISESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG KGKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSKGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD ATLKNILIVSNEEERR MIEEVGEEVLP EYGGKAKFI LQDAVLPH
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVSNEEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPH 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. ExPASy TrEMBL
Match: A0A6J1K6Q5 (SEC14 cytosolic factor-like OS=Cucurbita maxima OX=3661 GN=LOC111491647 PE=4 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 7.2e-117
Identity = 214/242 (88.43%), Postives = 225/242 (92.98%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DSRANALIQMR+++EKSGI+TQEYCDATMMRFLIARSM+PSKGAKMFVQWR WRDAMV
Sbjct: 1   MEDSRANALIQMRKAVEKSGINTQEYCDATMMRFLIARSMEPSKGAKMFVQWRTWRDAMV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKGF+ ESEV EELEA+KIFLQGLSK  L VMIVMA RHF SKDQLQFKKFLVHLLDKVI
Sbjct: 61  PKGFICESEVKEELEAKKIFLQGLSKTGLAVMIVMAKRHFSSKDQLQFKKFLVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASG +GKEIGNEKWIA+VDL QISYKNVD RGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGSEGKEIGNEKWIAIVDLHQISYKNVDIRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD ATLKNILIV N+EERR MIEEVGEEVLP EYGGKAKFI LQDAVLP 
Sbjct: 181 VSIWRMVSRFVDPATLKNILIVRNKEERRSMIEEVGEEVLPEEYGGKAKFIALQDAVLPR 240

Query: 241 LD 243
           LD
Sbjct: 241 LD 242

BLAST of Spg028816 vs. ExPASy TrEMBL
Match: A0A0A0KL83 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G021320 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.6e-114
Identity = 204/243 (83.95%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+ S ANA+IQ+R+SI KSG STQ+Y DATMMRFLIARSM+  K AKMFVQW+KWRDA V
Sbjct: 1   MEGSSANAVIQLRDSIHKSGFSTQDYSDATMMRFLIARSMEVPKAAKMFVQWKKWRDATV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKG ++ESEV +EL+A+KIFLQG+S K+LPVMIVMANRHF SKDQ+QFKKF+VHLLDKVI
Sbjct: 61  PKGLIAESEVEDELKAKKIFLQGISIKQLPVMIVMANRHFHSKDQVQFKKFIVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIA+VDLQQISYKNVDPRGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRM+SRFVD+ATLKNILIVS+EEE+RIMIEEVGEEVLP+EYGGKAKFI LQD VLPH
Sbjct: 181 VSIWRMISRFVDTATLKNILIVSSEEEKRIMIEEVGEEVLPIEYGGKAKFIVLQDVVLPH 240

Query: 241 LDG 244
           L G
Sbjct: 241 LHG 243

BLAST of Spg028816 vs. ExPASy TrEMBL
Match: A0A6J1CY34 (sec14 cytosolic factor-like OS=Momordica charantia OX=3673 GN=LOC111015872 PE=4 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 1.3e-113
Identity = 210/243 (86.42%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M++SRANAL QMR+S+EK G ST+ Y DATMMRFLIARSM+P+KGAKMFVQWRKWR+AMV
Sbjct: 1   MEESRANALFQMRKSVEKLGFSTEAYGDATMMRFLIARSMEPAKGAKMFVQWRKWREAMV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           P+G V ESEV EELEARKIFLQG SKK L V+IV A+RHFPSKDQLQFKKF+VHLLDKVI
Sbjct: 61  PEGSVCESEVAEELEARKIFLQGSSKKGLAVVIVKASRHFPSKDQLQFKKFVVHLLDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKG+EIGNEKWIA+VDL QISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGREIGNEKWIAIVDLHQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDA-VLP 240
           VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVG EVLPVEYGGKAK I LQDA ++P
Sbjct: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVG-EVLPVEYGGKAKLIALQDAIIIP 240

Query: 241 HLD 243
           HLD
Sbjct: 241 HLD 242

BLAST of Spg028816 vs. ExPASy TrEMBL
Match: A0A1S3BT62 (sec14 cytosolic factor-like OS=Cucumis melo OX=3656 GN=LOC103493455 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.3e-113
Identity = 201/241 (83.40%), Postives = 224/241 (92.95%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M+DS ANA+  +R SIEKSG STQ+Y DATMMRFLIARSM+ SK AKMFVQW+KWRDA V
Sbjct: 1   MEDSNANAVTLLRNSIEKSGFSTQDYSDATMMRFLIARSMEVSKAAKMFVQWKKWRDATV 60

Query: 61  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 120
           PKG+++ESEV +EL+A+KIFLQG+S K+LPVMIV+ANRHF SKDQLQFKKF+VH+LDKVI
Sbjct: 61  PKGYIAESEVEDELKAKKIFLQGMSIKQLPVMIVIANRHFHSKDQLQFKKFVVHILDKVI 120

Query: 121 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFF 180
           ASGCKGKEIGNEKWIA+VDLQQISYKNVDPRGLIT FQFLQNYYPERLGKCFILNMPWFF
Sbjct: 121 ASGCKGKEIGNEKWIAIVDLQQISYKNVDPRGLITAFQFLQNYYPERLGKCFILNMPWFF 180

Query: 181 VSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLPH 240
           VSIWRMVSRFVD+ATLKNILIVSNEEE+R MIEEVGE+VLP+EYGGKAKF  LQD V+PH
Sbjct: 181 VSIWRMVSRFVDTATLKNILIVSNEEEKRNMIEEVGEDVLPIEYGGKAKFFALQDVVVPH 240

Query: 241 L 242
           L
Sbjct: 241 L 241

BLAST of Spg028816 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 285.4 bits (729), Expect = 4.2e-77
Identity = 137/240 (57.08%), Postives = 184/240 (76.67%), Query Frame = 0

Query: 1   MDDSRANALIQMRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 60
           M++S+  AL Q+R+S+EK   ST+ Y   T+MRFL+ARSMDP K AKMFV W+KWR +MV
Sbjct: 1   MEESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMV 60

Query: 61  -PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKV 120
            P GF+ ESEV +ELE RK+ LQG +K   P+++V+ ++HF SKD   FKKF+V+ LDK 
Sbjct: 61  PPTGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKT 120

Query: 121 IASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWF 180
           IASG  GKE+G EK +A++DL  I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP F
Sbjct: 121 IASGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGF 180

Query: 181 FVSIWRMVSRFVDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLP 240
           FV++W+ V RF++ AT + I+IV++ EE+R   EE+G + LP EYGG+AK   +QD +LP
Sbjct: 181 FVTVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 240

BLAST of Spg028816 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 127/219 (57.99%), Postives = 168/219 (76.71%), Query Frame = 0

Query: 22  STQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV-PKGFVSESEVMEELEARKIF 81
           S + Y   T+MRFL+ARSMDP K AKMFV W+KWR +MV P GF+ ESEV +ELE RK+ 
Sbjct: 9   SEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVC 68

Query: 82  LQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVIASGCKGKEIGNEKWIAMVDL 141
           LQG +K   P+++V+ ++HF SKD   FKKF+V+ LDK IASG  GKE+G EK +A++DL
Sbjct: 69  LQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDL 128

Query: 142 QQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNIL 201
             I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++ AT + I+
Sbjct: 129 ANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIV 188

Query: 202 IVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLP 240
           IV++ EE+R   EE+G + LP EYGG+AK   +QD +LP
Sbjct: 189 IVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of Spg028816 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-71
Identity = 127/219 (57.99%), Postives = 168/219 (76.71%), Query Frame = 0

Query: 22  STQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV-PKGFVSESEVMEELEARKIF 81
           S + Y   T+MRFL+ARSMDP K AKMFV W+KWR +MV P GF+ ESEV +ELE RK+ 
Sbjct: 9   SEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVC 68

Query: 82  LQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVIASGCKGKEIGNEKWIAMVDL 141
           LQG +K   P+++V+ ++HF SKD   FKKF+V+ LDK IASG  GKE+G EK +A++DL
Sbjct: 69  LQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDL 128

Query: 142 QQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRFVDSATLKNIL 201
             I+YKN+D RGLITGFQFLQ+YYPERL KC+IL+MP FFV++W+ V RF++ AT + I+
Sbjct: 129 ANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIV 188

Query: 202 IVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQDAVLP 240
           IV++ EE+R   EE+G + LP EYGG+AK   +QD +LP
Sbjct: 189 IVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQDVLLP 227

BLAST of Spg028816 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 165.2 bits (417), Expect = 6.4e-41
Identity = 82/225 (36.44%), Postives = 135/225 (60.00%), Query Frame = 0

Query: 12  MRESIEKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMVPKGFVSESEVM 71
           MR   ++    T+E  D  + RFL AR +D  K + MF+ +  W+ +M+PKG + E+E+ 
Sbjct: 34  MRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIA 93

Query: 72  EELEARKIFLQGLSKKELPVMIVMANRHFPSK-DQLQFKKFLVHLLDKVIASGCKGKEIG 131
            +L   K+ +QG  K   P+ + + NRH PSK +  +FK+F+V+ L+K+    C     G
Sbjct: 94  NDLSHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKI----CARMPRG 153

Query: 132 NEKWIAMVDLQQISYKNVDPRGLITGFQFLQNYYPERLGKCFILNMPWFFVSIWRMVSRF 191
            EK++A+ DLQ   Y N D RG +     LQ+ YPERLGK +I++ P+ F++ W+++  F
Sbjct: 154 QEKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPF 213

Query: 192 VDSATLKNILIVSNEEERRIMIEEVGEEVLPVEYGGKAKFIPLQD 236
           +D+ T K I+ V N++    ++E++ E  LP  YGGK   +P+Q+
Sbjct: 214 IDANTKKKIVFVENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of Spg028816 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 80.5 bits (197), Expect = 2.1e-15
Identity = 69/232 (29.74%), Postives = 109/232 (46.98%), Query Frame = 0

Query: 2   DDSRANALIQMRESI-EKSGISTQEYCDATMMRFLIARSMDPSKGAKMFVQWRKWRDAMV 61
           D  + N + +++ +I   SG S     DA++ RFL AR+ D  K  KM  +  KWR    
Sbjct: 14  DSQQDNKVRELKSAIGPLSGHSLVFCSDASLRRFLDARNWDVEKAKKMIQETLKWRSTYK 73

Query: 62  PKGFVSESEVMEELEARKIFLQGLSKKELPVMIVMANRHFPSKDQLQFKKFLVHLLDKVI 121
           P+  +  ++V  E E  K        ++  V+++M      S  Q    + LV+LL+  I
Sbjct: 74  PQE-IRWNQVAHEGETGKASRASFHDRQGRVVLIMRPAMQNSTSQEGNIRHLVYLLENAI 133

Query: 122 ASGCKGKEIGNEKWIAMVDLQQISYKNVDPRGLITG--FQFLQNYYPERLGKCFILNMPW 181
            +  KG++     W  ++D    S   V+P    T      LQNYYPERLG  F+ N P 
Sbjct: 134 INLPKGQK--QMSW--LIDFTGWSMA-VNPPMKTTREIIHILQNYYPERLGIAFLYNPPR 193

Query: 182 FFVSIWRMVSRFVDSATLKNILIV--SNEEERRIMIEEVGEEVLPVEYGGKA 229
            F +++R    F+D  T + +  V   ++    +M      E LP E+GG+A
Sbjct: 194 LFQAVYRAAKYFLDPRTAEKVKFVYPKDKASDELMTTHFDVENLPKEFGGEA 239