Spg027622 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg027622
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Protein TIC 20
Location	scaffold6: 37820658 .. 37821601 (-)
RNA-Seq Expression	Spg027622
Synteny	Spg027622

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCATCGCCATTTCCTTCGCTCTCAAACCCTAGCAGAAGAATCCTCACATCCCAAATCCACTTCAGAACTCCACGACGACCATTCCTCACCGTCCGATCCTCCGGCGACAACAGCAACAACTCCGCCGACGGCCCCGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTCATTCAGCCCTTGGTTCCCGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATGGATTTTTGGTCTTCTTGACTCTTTACTTCGTCGTTGTCAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCGATTGTTCTTGATGTGCTCCTGATTTTCCCTGATCTTTTGGAGAGGAGCTTTAATCCCAGAGGGGGATTTGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTTCTGTTTCTTTTGGTTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATTCCTAGATTGCCCATTGTTGCTGAAGCTGCTGATAGGCAAGTTATGTGAATTCTTGAGAAATTCTTCTGGATGAGTTTAAGCTTCTGCTATTCTTCATTCCTTTGCTGATTTGTTGGGGAAATTTGGGTGGAATTTAGAAAAACTTCCTTTGGTACTTTGTCAGTAGATTGGTTCACTGTTTCATATCTGTTGTTCCTAAACAGACTGATGAGGATGAAATGATTAAAGAAAAACAAGTTAGATATAATCTTCAATGTCCATTTTTTCTGCTTTCTGTTGCTGAAAGCCTTGCCATTGTTTCTGAGGGAGGCAAGTGTTCTGATATGGGTTTGCTAATTCTTGCCACTATTTCTGATTGA

mRNA sequence

ATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCATCGCCATTTCCTTCGCTCTCAAACCCTAGCAGAAGAATCCTCACATCCCAAATCCACTTCAGAACTCCACGACGACCATTCCTCACCGTCCGATCCTCCGGCGACAACAGCAACAACTCCGCCGACGGCCCCGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTCATTCAGCCCTTGGTTCCCGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATGGATTTTTGGTCTTCTTGACTCTTTACTTCGTCGTTGTCAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCGATTGTTCTTGATGTGCTCCTGATTTTCCCTGATCTTTTGGAGAGGAGCTTTAATCCCAGAGGGGGATTTGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTTCTGTTTCTTTTGGTTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATTCCTAGATTGCCCATTGTTGCTGAAGCTGCTGATAGGCAAACTGATGAGGATGAAATGATTAAAGAAAAACAAGTTAGATATAATCTTCAATGTCCATTTTTTCTGCTTTCTGTTGCTGAAAGCCTTGCCATTGTTTCTGAGGGAGGCAAGTGTTCTGATATGGGTTTGCTAATTCTTGCCACTATTTCTGATTGA

Coding sequence (CDS)

ATGCTTATCTTAAATCTCTCCCCCAAAACCCTCCATTCTTCCTTCTTCCCATCGCCATTTCCTTCGCTCTCAAACCCTAGCAGAAGAATCCTCACATCCCAAATCCACTTCAGAACTCCACGACGACCATTCCTCACCGTCCGATCCTCCGGCGACAACAGCAACAACTCCGCCGACGGCCCCGACCGCCTCATCTCCGCCATCTGCTACTTCTACCCCTTCTTCGACGGCGTCCAGTACGGCAAGTACGTTATCACTCAGTACGCTCCCTTTCAGGTTCTCATTCAGCCCTTGGTTCCCGCCATTCGAGTTTTCAAGAGCTTTCCATTGAATGGATTTTTGGTCTTCTTGACTCTTTACTTCGTCGTTGTCAGAAACCCTAATTTCAGTCGCTATGTTAGGTTCAATATTATGCAAGCGATTGTTCTTGATGTGCTCCTGATTTTCCCTGATCTTTTGGAGAGGAGCTTTAATCCCAGAGGGGGATTTGGGTTGGATTTGGTCATGAGTTTGGATAGTACTGTGTTTCTGTTTCTTTTGGTTTGTTTGATTTATGGCTCTTCTTCTTGCTTGTTGGGTCAGATTCCTAGATTGCCCATTGTTGCTGAAGCTGCTGATAGGCAAACTGATGAGGATGAAATGATTAAAGAAAAACAAGTTAGATATAATCTTCAATGTCCATTTTTTCTGCTTTCTGTTGCTGAAAGCCTTGCCATTGTTTCTGAGGGAGGCAAGTGTTCTGATATGGGTTTGCTAATTCTTGCCACTATTTCTGATTGA

Protein sequence

MLILNLSPKTLHSSFFPSPFPSLSNPSRRILTSQIHFRTPRRPFLTVRSSGDNSNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDSTVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQTDEDEMIKEKQVRYNLQCPFFLLSVAESLAIVSEGGKCSDMGLLILATISD

Homology

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BLAST of Spg027622 vs. NCBI nr
Match: XP_023553383.1 (protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.2 bits (931), Expect = 1.8e-96
Identity = 189/214 (88.32%), Postives = 196/214 (91.59%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP---SRRILTSQIHF----RTPRRPFLTVRSSGDNS 61
           LILNLSPKTLHSSFFPSPFPSLSNP   +   LT+QI+F    R  RR  LTVRSSGD  
Sbjct: 25  LILNLSPKTLHSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSGDRD 84

Query: 62  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFL 121
           NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGFL
Sbjct: 85  NNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFL 144

Query: 122 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDST 181
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDST
Sbjct: 145 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST 204

Query: 182 VFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           VFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 205 VFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 238

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BLAST of Spg027622 vs. NCBI nr
Match: XP_022932396.1 (protein TIC 20-v, chloroplastic [Cucurbita moschata])

HSP 1 Score: 362.8 bits (930), Expect = 2.4e-96
Identity = 189/215 (87.91%), Postives = 196/215 (91.16%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP----SRRILTSQIHF----RTPRRPFLTVRSSGDN 61
           LILNLSPKTLHSSFFPSPFPSLSNP    +   LT+QI+F    R  RR  LTVRSSGD 
Sbjct: 26  LILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSSGDR 85

Query: 62  SNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGF 121
            NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGF
Sbjct: 86  DNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGF 145

Query: 122 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDS 181
           LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDS
Sbjct: 146 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDS 205

Query: 182 TVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           TVFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 206 TVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 240

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BLAST of Spg027622 vs. NCBI nr
Match: KAG6577521.1 (Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015580.1 Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 360.9 bits (925), Expect = 9.1e-96
Identity = 188/215 (87.44%), Postives = 195/215 (90.70%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP----SRRILTSQIHF----RTPRRPFLTVRSSGDN 61
           LILNLSPKTLHSSFFPSPFPSL NP    +   LT+QI+F    R  RR  LTVRSSGD 
Sbjct: 4   LILNLSPKTLHSSFFPSPFPSLPNPRTTATTTTLTNQINFTTLRRRRRRRILTVRSSGDR 63

Query: 62  SNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGF 121
            NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGF
Sbjct: 64  DNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGF 123

Query: 122 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDS 181
           LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDS
Sbjct: 124 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDS 183

Query: 182 TVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           TVFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 184 TVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 218

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BLAST of Spg027622 vs. NCBI nr
Match: XP_022965178.1 (protein TIC 20-v, chloroplastic [Cucurbita maxima])

HSP 1 Score: 360.1 bits (923), Expect = 1.5e-95
Identity = 188/214 (87.85%), Postives = 195/214 (91.12%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP---SRRILTSQIHF----RTPRRPFLTVRSSGDNS 61
           LILNLSPKTL SSFFPSPFPSLSNP   +   LT+QI+F    R  RR  LTVRSSGD  
Sbjct: 4   LILNLSPKTLRSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSGDRD 63

Query: 62  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFL 121
           NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGFL
Sbjct: 64  NNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFL 123

Query: 122 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDST 181
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDST
Sbjct: 124 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST 183

Query: 182 VFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           VFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 184 VFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 217

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BLAST of Spg027622 vs. NCBI nr
Match: XP_038905184.1 (protein TIC 20-v, chloroplastic [Benincasa hispida])

HSP 1 Score: 357.8 bits (917), Expect = 7.7e-95
Identity = 187/209 (89.47%), Postives = 196/209 (93.78%), Query Frame = 0


Query: 1   MLILNLSPKTLHSSFFPSPFPSLSNPSRRILTSQIHFRTPRRPFLTVRSSGDNSN-NSAD 60
           MLILNLSPKTL+SSFFPSPFPSLSNP++   T+ +  R  RR  LTVRS+GDN+N NSAD
Sbjct: 3   MLILNLSPKTLYSSFFPSPFPSLSNPTQINFTATLPRR--RRRILTVRSTGDNNNSNSAD 62

Query: 61  GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTL 120
           GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLN FLVFLTL
Sbjct: 63  GPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNSFLVFLTL 122

Query: 121 YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDSTVFLFL 180
           YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDSTVFLFL
Sbjct: 123 YFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDSTVFLFL 182

Query: 181 LVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           L+CLIYGSSSCLLGQIPRLPIVA+AADRQ
Sbjct: 183 LICLIYGSSSCLLGQIPRLPIVADAADRQ 209

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BLAST of Spg027622 vs. ExPASy Swiss-Prot
Match: Q9FM67 (Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.0e-70
Identity = 142/207 (68.60%), Postives = 160/207 (77.29%), Query Frame = 0


Query: 13  SSFFPSPFPSLSN----PSRRILTSQIHFRTP-------RRPFLTVRSSGDNSNNSADGP 72
           S FF +P PSL+       R  L   +  + P       R   L ++S GD   +S D  
Sbjct: 5   SQFF-APLPSLTGTLTLTGRSFLPLNLDTQFPKPRLSRDRAATLVLQSKGD---DSVDAS 64

Query: 73  DRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTLYF 132
           DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ+LIQPL PAIR FKSFP NGFL+F+TLYF
Sbjct: 65  DRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITLYF 124

Query: 133 VVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDSTVFLFLLV 192
           VVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERSFNPR GFGLD+VMSLDSTVFLFLLV
Sbjct: 125 VVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVFLFLLV 184

Query: 193 CLIYGSSSCLLGQIPRLPIVAEAADRQ 209
            LIYG S+CL G  PRLP+VAEAADRQ
Sbjct: 185 SLIYGFSACLFGLTPRLPLVAEAADRQ 207

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BLAST of Spg027622 vs. ExPASy Swiss-Prot
Match: O82251 (Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.3e-30
Identity = 80/189 (42.33%), Postives = 109/189 (57.67%), Query Frame = 0


Query: 22  SLSNPSRRILTSQIHFRTPRRPFLTVRSSGDNSNNSADGPDRLISAICYFYPFFDGVQYG 81
           SLS P     T  I   + R   LT R+    S       +R+IS   Y  PFF+ +QYG
Sbjct: 25  SLSFPGSS--TFSIRPSSRRATALTTRA----SYTPTPATERVISIASYALPFFNSLQYG 84

Query: 82  KYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAI 141
           +++  QY    +L +P+ P + +++S P   F+ F  LY  VVRN +FSRYVRFN MQA+
Sbjct: 85  RFLFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAV 144

Query: 142 VLDVLLIFPDLLERSFNP--RGGFGLDLVMSLDSTVFLFLLVCLIYGSSSCLLGQIPRLP 201
            LDVLL  P LL R  +P   GGFG+  +M   + VF+F  +C +YG  S LLG+ P +P
Sbjct: 145 TLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIP 204

Query: 202 IVAEAADRQ 209
            VA+AA RQ
Sbjct: 205 FVADAAGRQ 207

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BLAST of Spg027622 vs. ExPASy Swiss-Prot
Match: P73387 (Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1737 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-20
Identity = 54/156 (34.62%), Postives = 91/156 (58.33%), Query Frame = 0


Query: 54  SNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGF 113
           SN++ADG DR  SA+ Y  P  D   +G +++ Q+   Q++  P++P ++ +  FP   F
Sbjct: 3   SNSTADGKDRFFSALIYVIPLIDAFMFGGFLLQQFPVLQIIYLPIMPLLQFYYQFPFASF 62

Query: 114 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLL-IFPDLLERSFNPRGGFGLDLVMSLD 173
           ++F+ L+  VVRN N S ++RFN MQAI++ +LL +F  ++     P  G GL +  +L 
Sbjct: 63  IIFIVLFMAVVRNNNISHFIRFNAMQAILIGILLSLFGLIVAYVIQPVFGQGL-VTETLY 122

Query: 174 STVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           +  FL  L C  +G    +LG+   +P +++AA  Q
Sbjct: 123 NFAFLGALACGFFGIVQSVLGRYAEIPTISDAAYSQ 157

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BLAST of Spg027622 vs. ExPASy Swiss-Prot
Match: O19916 (Tic20 family protein Ycf60 OS=Cyanidium caldarium OX=2771 GN=ycf60 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 3.3e-08
Identity = 44/150 (29.33%), Postives = 79/150 (52.67%), Query Frame = 0


Query: 63  RLISAICYFYPFFDGV-QYGKYVITQYAPFQVLIQPLVPAIRV-FKSFPLNGFLVFLTLY 122
           R IS + YF P  +G+ Q+G   I  ++  +++ +  +  I V +   PL GF +F+TLY
Sbjct: 53  RAISCLIYFLPLLEGIAQFGIVCIDDHSWIRIIYKNTLAYIVVPYLESPLIGFCIFITLY 112

Query: 123 FVVVRN-PNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDSTVFLFL 182
            + VR     S++++F+I+QA++L +L      +  S     G+   +   L  T+ L  
Sbjct: 113 LIFVRGIIQISKFIKFHIVQALLLYLLDSVIGTVLTSLPLEIGYSF-IGDRLADTLLLIT 172

Query: 183 LVCLIYGSSSCLLGQIPRLPIVAEAADRQT 210
            +  IY  +  L GQ   +P+++EAA  Q+
Sbjct: 173 FMISIYAGTDALKGQYSNIPLISEAAKMQS 201

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BLAST of Spg027622 vs. ExPASy Swiss-Prot
Match: Q85G30 (Tic20 family protein Ycf60 OS=Cyanidioschyzon merolae (strain 10D) OX=280699 GN=ycf60 PE=3 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.8e-04
Identity = 34/114 (29.82%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 104 VFKSFPLNGFLVFLTLYFVVVRN-PNFSRYVRFNIMQAIVLDVLL-IFPDLLER--SFNP 163
           V+ ++P+ GF++F   YF++VR      + VRF++ QA+++ +L  I   LL        
Sbjct: 84  VYSTYPILGFMIFFMSYFLLVRGILQVRKKVRFHVSQALIIYLLTSIIGSLLNALPEMIL 143

Query: 164 RGGFGLDLVMSLDSTVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQTDEDE 214
            G FG   +      +F+  +  +IY S     G++ RLP+++EAA  Q  + E
Sbjct: 144 MGWFGSTCL----DILFILTMGSVIYASYQVWNGELTRLPLISEAAKLQVQDGE 193

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BLAST of Spg027622 vs. ExPASy TrEMBL
Match: A0A6J1EW92 (Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 1.2e-96
Identity = 189/215 (87.91%), Postives = 196/215 (91.16%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP----SRRILTSQIHF----RTPRRPFLTVRSSGDN 61
           LILNLSPKTLHSSFFPSPFPSLSNP    +   LT+QI+F    R  RR  LTVRSSGD 
Sbjct: 26  LILNLSPKTLHSSFFPSPFPSLSNPRTTAATTTLTNQINFTTLRRRRRRRILTVRSSGDR 85

Query: 62  SNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGF 121
            NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGF
Sbjct: 86  DNNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGF 145

Query: 122 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDS 181
           LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDS
Sbjct: 146 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDS 205

Query: 182 TVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           TVFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 206 TVFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 240

[Close]

BLAST of Spg027622 vs. ExPASy TrEMBL
Match: A0A6J1HN42 (Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 7.5e-96
Identity = 188/214 (87.85%), Postives = 195/214 (91.12%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNP---SRRILTSQIHF----RTPRRPFLTVRSSGDNS 61
           LILNLSPKTL SSFFPSPFPSLSNP   +   LT+QI+F    R  RR  LTVRSSGD  
Sbjct: 4   LILNLSPKTLRSSFFPSPFPSLSNPRTTATTTLTNQINFTTLRRRRRRRILTVRSSGDRD 63

Query: 62  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFL 121
           NN+ADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFP NGFL
Sbjct: 64  NNTADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPFNGFL 123

Query: 122 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDST 181
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDST
Sbjct: 124 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDST 183

Query: 182 VFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           VFLFLL+CLIYGSSSCLLGQ+PRLPIVA+AADRQ
Sbjct: 184 VFLFLLICLIYGSSSCLLGQVPRLPIVADAADRQ 217

[Close]

BLAST of Spg027622 vs. ExPASy TrEMBL
Match: A0A5D3CPD4 (Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 185/214 (86.45%), Postives = 193/214 (90.19%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNPSRRILTSQIHFRTP-------RRPFLTVRSSGDNS 61
           LILNLSPKTLHSSFFPSPFP+LSNP     T+ ++F T        RR   TVRS+GDN+
Sbjct: 4   LILNLSPKTLHSSFFPSPFPALSNPK----TTTLNFTTTLRRRGRGRRRIFTVRSTGDNN 63

Query: 62  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFL 121
           NNSADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSFPLN FL
Sbjct: 64  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFL 123

Query: 122 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDST 181
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDST
Sbjct: 124 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLVMSLDST 183

Query: 182 VFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           VFLFLL+CLIYGSSSCLLGQIPRLPIVA+AADRQ
Sbjct: 184 VFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQ 213

[Close]

BLAST of Spg027622 vs. ExPASy TrEMBL
Match: A0A1S3BM16 (Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 185/214 (86.45%), Postives = 193/214 (90.19%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNPSRRILTSQIHFRTP-------RRPFLTVRSSGDNS 61
           LILNLSPKTLHSSFFPSPFP+LSNP     T+ ++F T        RR   TVRS+GDN+
Sbjct: 4   LILNLSPKTLHSSFFPSPFPALSNPK----TTTLNFTTTLRRRGRGRRRIFTVRSTGDNN 63

Query: 62  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFL 121
           NNSADGPDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSFPLN FL
Sbjct: 64  NNSADGPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSFL 123

Query: 122 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDST 181
           VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDST
Sbjct: 124 VFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGIGLDLVMSLDST 183

Query: 182 VFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           VFLFLL+CLIYGSSSCLLGQIPRLPIVA+AADRQ
Sbjct: 184 VFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQ 213

[Close]

BLAST of Spg027622 vs. ExPASy TrEMBL
Match: A0A0A0L632 (Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.9e-87
Identity = 181/215 (84.19%), Postives = 188/215 (87.44%), Query Frame = 0


Query: 2   LILNLSPKTLHSSFFPSPFPSLSNPSRRILTSQIHFRT--PRR-----PFLTVRSSG-DN 61
           LILNLSPKT HS F    FPSLSNP     T+ ++F T  PRR       LTVRS+G DN
Sbjct: 4   LILNLSPKTPHSPF----FPSLSNPK----TTTLNFTTTLPRRWLARPRILTVRSTGDDN 63

Query: 62  SNNSADGPDRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGF 121
           +NNSAD PDRLISAICYFYPFFDGVQYGKYVITQY PFQVLIQPLVPAIRVFKSFPLN F
Sbjct: 64  NNNSADAPDRLISAICYFYPFFDGVQYGKYVITQYTPFQVLIQPLVPAIRVFKSFPLNSF 123

Query: 122 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDS 181
           LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGG GLDLVMSLDS
Sbjct: 124 LVFLTLYFVVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGLGLDLVMSLDS 183

Query: 182 TVFLFLLVCLIYGSSSCLLGQIPRLPIVAEAADRQ 209
           TVFLFLL+CLIYGSSSCLLGQIPRLPIVA+AADRQ
Sbjct: 184 TVFLFLLICLIYGSSSCLLGQIPRLPIVADAADRQ 210

[Close]

BLAST of Spg027622 vs. TAIR 10
Match: AT5G55710.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: Uncharacterised conserved protein ycf60 (TAIR:AT2G47840.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 266.2 bits (679), Expect = 2.8e-71
Identity = 142/207 (68.60%), Postives = 160/207 (77.29%), Query Frame = 0


Query: 13  SSFFPSPFPSLSN----PSRRILTSQIHFRTP-------RRPFLTVRSSGDNSNNSADGP 72
           S FF +P PSL+       R  L   +  + P       R   L ++S GD   +S D  
Sbjct: 5   SQFF-APLPSLTGTLTLTGRSFLPLNLDTQFPKPRLSRDRAATLVLQSKGD---DSVDAS 64

Query: 73  DRLISAICYFYPFFDGVQYGKYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTLYF 132
           DR+ISA+CYFYPFFDG+QYGK++ITQY PFQ+LIQPL PAIR FKSFP NGFL+F+TLYF
Sbjct: 65  DRIISAVCYFYPFFDGIQYGKFIITQYQPFQILIQPLFPAIRAFKSFPFNGFLIFITLYF 124

Query: 133 VVVRNPNFSRYVRFNIMQAIVLDVLLIFPDLLERSFNPRGGFGLDLVMSLDSTVFLFLLV 192
           VVVRNPNFSRYVRFN MQAIVLDVLLIFPDLLERSFNPR GFGLD+VMSLDSTVFLFLLV
Sbjct: 125 VVVRNPNFSRYVRFNTMQAIVLDVLLIFPDLLERSFNPRDGFGLDVVMSLDSTVFLFLLV 184

Query: 193 CLIYGSSSCLLGQIPRLPIVAEAADRQ 209
            LIYG S+CL G  PRLP+VAEAADRQ
Sbjct: 185 SLIYGFSACLFGLTPRLPLVAEAADRQ 207

[Close]

BLAST of Spg027622 vs. TAIR 10
Match: AT2G47840.1 (Uncharacterised conserved protein ycf60 )

HSP 1 Score: 132.9 bits (333), Expect = 3.7e-31
Identity = 80/189 (42.33%), Postives = 109/189 (57.67%), Query Frame = 0


Query: 22  SLSNPSRRILTSQIHFRTPRRPFLTVRSSGDNSNNSADGPDRLISAICYFYPFFDGVQYG 81
           SLS P     T  I   + R   LT R+    S       +R+IS   Y  PFF+ +QYG
Sbjct: 25  SLSFPGSS--TFSIRPSSRRATALTTRA----SYTPTPATERVISIASYALPFFNSLQYG 84

Query: 82  KYVITQYAPFQVLIQPLVPAIRVFKSFPLNGFLVFLTLYFVVVRNPNFSRYVRFNIMQAI 141
           +++  QY    +L +P+ P + +++S P   F+ F  LY  VVRN +FSRYVRFN MQA+
Sbjct: 85  RFLFAQYPRLGLLFEPIFPILNLYRSVPYASFVAFFGLYLGVVRNTSFSRYVRFNAMQAV 144

Query: 142 VLDVLLIFPDLLERSFNP--RGGFGLDLVMSLDSTVFLFLLVCLIYGSSSCLLGQIPRLP 201
            LDVLL  P LL R  +P   GGFG+  +M   + VF+F  +C +YG  S LLG+ P +P
Sbjct: 145 TLDVLLAVPVLLTRILDPGQGGGFGMKAMMWGHTGVFVFSFMCFVYGVVSSLLGKTPYIP 204

Query: 202 IVAEAADRQ 209
            VA+AA RQ
Sbjct: 205 FVADAAGRQ 207

The following BLAST results are available for this feature:

BLAST of Spg027622 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553383.1	1.8e-96	88.32	protein TIC 20-v, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022932396.1	2.4e-96	87.91	protein TIC 20-v, chloroplastic [Cucurbita moschata]	[more]
KAG6577521.1	9.1e-96	87.44	Protein TIC 20-v, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]...	[more]
XP_022965178.1	1.5e-95	87.85	protein TIC 20-v, chloroplastic [Cucurbita maxima]	[more]
XP_038905184.1	7.7e-95	89.47	protein TIC 20-v, chloroplastic [Benincasa hispida]	[more]

BLAST of Spg027622 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FM67	4.0e-70	68.60	Protein TIC 20-v, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-V PE=2 ...	[more]
O82251	5.3e-30	42.33	Protein TIC 20-II, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC20-II PE=...	[more]
P73387	1.0e-20	34.62	Tic20 family protein OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 ...	[more]
O19916	3.3e-08	29.33	Tic20 family protein Ycf60 OS=Cyanidium caldarium OX=2771 GN=ycf60 PE=3 SV=1	[more]
Q85G30	3.8e-04	29.82	Tic20 family protein Ycf60 OS=Cyanidioschyzon merolae (strain 10D) OX=280699 GN=...	[more]

BLAST of Spg027622 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EW92	1.2e-96	87.91	Protein TIC 20 OS=Cucurbita moschata OX=3662 GN=LOC111438760 PE=3 SV=1	[more]
A0A6J1HN42	7.5e-96	87.85	Protein TIC 20 OS=Cucurbita maxima OX=3661 GN=LOC111465112 PE=3 SV=1	[more]
A0A5D3CPD4	1.1e-94	86.45	Protein TIC 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00160...	[more]
A0A1S3BM16	1.1e-94	86.45	Protein TIC 20 OS=Cucumis melo OX=3656 GN=LOC103491027 PE=3 SV=1	[more]
A0A0A0L632	2.9e-87	84.19	Protein TIC 20 OS=Cucumis sativus OX=3659 GN=Csa_3G047830 PE=3 SV=1	[more]

BLAST of Spg027622 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55710.1	2.8e-71	68.60	FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow...	[more]
AT2G47840.1	3.7e-31	42.33	Uncharacterised conserved protein ycf60	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005691	Chloroplast protein import component Tic20	PFAM	PF16166	TIC20	coord: 63..205 e-value: 8.4E-55 score: 185.3
IPR005691	Chloroplast protein import component Tic20	PANTHER	PTHR33510	PROTEIN TIC 20-II, CHLOROPLASTIC	coord: 29..209
None	No IPR available	PANTHER	PTHR33510:SF11	PROTEIN TIC 20-V, CHLOROPLASTIC	coord: 29..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg027622.1	Spg027622.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009579	thylakoid