Homology
BLAST of Spg027204 vs. NCBI nr
Match:
KAG6605277.1 (Meiosis regulator and mRNA stability factor 1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1577.8 bits (4084), Expect = 0.0e+00
Identity = 826/1029 (80.27%), Postives = 896/1029 (87.07%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFSQFS S SS N+SF SSSSSRRHDEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRALLLHFSQFSASFSSSNYSFPSSSSSRRHDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 YVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+APGVL SAASIMWHWNAL++GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESAPGVLCSAASIMWHWNALVKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP PIP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQASSIRSEEVSEISSDPKPLPIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQ 360
S+DRD+YGYKKFSRFLLSMPH LKLQTNGDGQ IV +VTPR IEPFESSR TS NGTE+Q
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHTLKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDNGTEEQ 360
Query: 361 DPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCPV 420
D NLIAKLNNNGSS ES SVP++ S ELNAQD+P KV+PS E KS G+ M GEPST PV
Sbjct: 361 DRNLIAKLNNNGSSPESTSVPLVRSSELNAQDRPEKVQPSYELVKSTGEAMGGEPSTWPV 420
Query: 421 SEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHNSENGSHHVS 480
SEPHV+EDSKQTSKFEA +N+ PSIGQHSE K GF RRIWRRF +GS DHNSENGSHH+S
Sbjct: 421 SEPHVMEDSKQTSKFEADNNMIPSIGQHSEAKTGFFRRIWRRFVVGSKDHNSENGSHHIS 480
Query: 481 EKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAKL 540
EKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSVH VSNSP+RE AKL
Sbjct: 481 EKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSEDANSVHPVSNSPDREPAKL 540
Query: 541 QRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQLF 600
Q+TA+VAS+Y DKS S PG+L SIRNWFK + NDTE K SE+CCEQNQLK++ KHQLF
Sbjct: 541 QKTAVVASAYDDKSRSRPGVLSSIRNWFKIRENDTETGKESEQCCEQNQLKNECGKHQLF 600
Query: 601 SSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLIS 660
S SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GPLVLKSLSTS+LFDF+E LIS
Sbjct: 601 SRSSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEEGPLVLKSLSTSELFDFIESLIS 660
Query: 661 DKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP----SPEHDSDS 720
DKKW+ ECPSETNPFK+TLSTA KSSCTKPLHRANGLTSIFMN+VSQP S EHDSDS
Sbjct: 661 DKKWLVECPSETNPFKVTLSTAEKSSCTKPLHRANGLTSIFMNRVSQPSLQGSSEHDSDS 720
Query: 721 DKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLEK 780
DKKNEN+PQAGISTTMTKSKFPERTRSEIL DCQ LVDEIL EHPEGYNMG F KLFLEK
Sbjct: 721 DKKNENVPQAGISTTMTKSKFPERTRSEILCDCQNLVDEILREHPEGYNMGAFRKLFLEK 780
Query: 781 YGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIHV 840
YGYHLDLQKLGYPKLASLLQIMPGVTI ST IVPT K PKVS HV
Sbjct: 781 YGYHLDLQKLGYPKLASLLQIMPGVTIESTFIVPTGKVPKVS----------------HV 840
Query: 841 VENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYEP 900
V NSD+ESSD PRKDDDFESTWEELGPA TDC RNE+EST S ETAEAT+++PKV YEP
Sbjct: 841 VANSDNESSDLPRKDDDFESTWEELGPAFTDCRSRNEDESTSSSETAEATEKRPKVCYEP 900
Query: 901 VLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSENS 960
V+ EDE TESDGESCPT QRSEE+AKP+T++EES LLQILDSWY +KE +SRK+ SENS
Sbjct: 901 VVLEDESSTESDGESCPTTQRSEEQAKPQTNKEESPLLQILDSWYGNKEHNSRKNNSENS 960
Query: 961 EEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTLK 1020
+EM DC ENSLK+SSL AK+EANTGSFARKQRHQKSYSFV DTDENDKEKLIDGIL TLK
Sbjct: 961 DEMNDCFENSLKVSSLTAKNEANTGSFARKQRHQKSYSFVLDTDENDKEKLIDGILGTLK 1008
Query: 1021 KSSESRIHN 1023
KSSESR+H+
Sbjct: 1021 KSSESRVHD 1008
BLAST of Spg027204 vs. NCBI nr
Match:
XP_022948172.1 (uncharacterized protein LOC111451828 [Cucurbita moschata])
HSP 1 Score: 1577.0 bits (4082), Expect = 0.0e+00
Identity = 826/1029 (80.27%), Postives = 896/1029 (87.07%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFSQFS S SS N+SF SSSSSRRHDEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRTLLLHFSQFSASFSSSNYSFPSSSSSRRHDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 YVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+APGVL SAASIMWHWNAL++GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESAPGVLCSAASIMWHWNALVKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP PIP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQVSSIRSEEVSEISSDPKPLPIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQ 360
S+DRD+YGYKKFSRFLLSMPH LKLQTNGDGQ IV +VTPR IEPFESSR TS NGTE+Q
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHTLKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDNGTEEQ 360
Query: 361 DPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCPV 420
D NLIAKLNNNGSS ES SVP++ S ELNAQD+P KV+PS E KS G+ M GEPST PV
Sbjct: 361 DRNLIAKLNNNGSSPESTSVPLVRSSELNAQDRPEKVQPSYELVKSTGEAMGGEPSTWPV 420
Query: 421 SEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHNSENGSHHVS 480
SEPHV+EDSKQTSKFEA +N+ PSIGQHSE K GF RRIWRRF +GS DHNSENGSHH+S
Sbjct: 421 SEPHVMEDSKQTSKFEADNNMIPSIGQHSEAKTGFFRRIWRRFVVGSKDHNSENGSHHIS 480
Query: 481 EKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAKL 540
EKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSVH VSNS +RE AKL
Sbjct: 481 EKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSQDANSVHPVSNSADREPAKL 540
Query: 541 QRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQLF 600
Q+TA+VAS+Y DKS S PG+L SIRNWFK + NDTE K SE+CCEQNQLK++ KHQLF
Sbjct: 541 QKTAVVASAYDDKSRSRPGVLSSIRNWFKIRENDTETGKESEQCCEQNQLKNECGKHQLF 600
Query: 601 SSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLIS 660
SS SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GPLVLKSLSTS+LFDF+E LIS
Sbjct: 601 SSSSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEEGPLVLKSLSTSELFDFIESLIS 660
Query: 661 DKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP----SPEHDSDS 720
DKKW+ ECPSETNPFK+TLSTA KSSCTKPLHRANGLTSIFMN+VSQP S EHDSDS
Sbjct: 661 DKKWLVECPSETNPFKVTLSTAEKSSCTKPLHRANGLTSIFMNRVSQPSLQGSSEHDSDS 720
Query: 721 DKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLEK 780
DKKNEN+PQAGISTTMTKSKFPERTRSEIL DCQ LVDEIL EHPEGYNMG F KLFLEK
Sbjct: 721 DKKNENVPQAGISTTMTKSKFPERTRSEILCDCQNLVDEILREHPEGYNMGAFRKLFLEK 780
Query: 781 YGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIHV 840
YGYHLDLQKLGYPKLASLLQIMPGV I ST IVPT K PKVS HV
Sbjct: 781 YGYHLDLQKLGYPKLASLLQIMPGVIIESTFIVPTGKVPKVS----------------HV 840
Query: 841 VENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYEP 900
V NSD+ESSD PRKDDDFESTWEELGPA TDC RNE+EST S ETAEAT+++PKV YEP
Sbjct: 841 VANSDNESSDLPRKDDDFESTWEELGPAFTDCRSRNEDESTSSSETAEATEKRPKVCYEP 900
Query: 901 VLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSENS 960
V+ EDE TESDGESCPT QRSEE+AKP+T++EES LLQILDSWY +KED+SRK+KSENS
Sbjct: 901 VVLEDESSTESDGESCPTTQRSEEQAKPQTNKEESPLLQILDSWYGNKEDNSRKNKSENS 960
Query: 961 EEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTLK 1020
+EM DC ENSLK+SSL AK+EANTGSFARK RHQKSYSFV DTDENDKEKLIDGIL TLK
Sbjct: 961 DEMNDCFENSLKVSSLTAKNEANTGSFARKHRHQKSYSFVLDTDENDKEKLIDGILGTLK 1008
Query: 1021 KSSESRIHN 1023
KSSESR+H+
Sbjct: 1021 KSSESRVHD 1008
BLAST of Spg027204 vs. NCBI nr
Match:
XP_023534760.1 (uncharacterized protein LOC111796232 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 822/1040 (79.04%), Postives = 893/1040 (85.87%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFSQFS S SS N+SF SSSSSRRHDEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRALLLHFSQFSASFSSSNYSFPSSSSSRRHDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 CVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+APGVL SAASIMWHWNAL++GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESAPGVLCSAASIMWHWNALVKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP PIP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQASSIRSEEVSEISSDPKPLPIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQ 360
S+DRD+YGYKKFSRFLLSMPH LKLQTNGDGQ IV +VTPR IEPFESSR TS +GTE+Q
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHTLKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDSGTEEQ 360
Query: 361 DPNLIAKLNNN---------GSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTM 420
D NLIAKLNNN GSS ES SVP++ S ELNAQD+P KV+PS E KS G+ M
Sbjct: 361 DRNLIAKLNNNGSSPESTSVGSSPESTSVPLVRSSELNAQDRPEKVQPSYELLKSTGEAM 420
Query: 421 EGEPSTCPVSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHN 480
GEPST VSEPHV+EDSKQ SKFEA +N+ PSIGQHSE K GF RRIWRRF +GS DHN
Sbjct: 421 GGEPST--VSEPHVMEDSKQNSKFEADNNMIPSIGQHSEAKTGFFRRIWRRFVVGSKDHN 480
Query: 481 SENGSHHVSEKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSN 540
SENGSHH+SEKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSV VSN
Sbjct: 481 SENGSHHISEKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSQDANSVRPVSN 540
Query: 541 SPERESAKLQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLK 600
P+RE AKLQ+TA+VAS+Y DKS S PGLL SIRNWFK NDTE K SE+CCEQNQLK
Sbjct: 541 LPDREPAKLQKTAVVASAYDDKSRSRPGLLSSIRNWFKIWENDTETGKESEQCCEQNQLK 600
Query: 601 DQSRKHQLFSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDL 660
++S KHQLFSS+SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GPLVLKSLSTS+L
Sbjct: 601 NESGKHQLFSSNSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEEGPLVLKSLSTSEL 660
Query: 661 FDFLELLISDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP--- 720
FDF+E LISDK W+ ECPSETNPFK+TLSTA KSSCTKPLHRANGLTSIFMN+VSQP
Sbjct: 661 FDFIESLISDKNWLVECPSETNPFKVTLSTAEKSSCTKPLHRANGLTSIFMNRVSQPSLQ 720
Query: 721 -SPEHDSDSDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMG 780
S EHDSDSDKKNEN+PQAGISTTMTKSKFPERTRSEIL DCQ LVDEIL EHPEGYNMG
Sbjct: 721 GSSEHDSDSDKKNENVPQAGISTTMTKSKFPERTRSEILCDCQNLVDEILREHPEGYNMG 780
Query: 781 TFGKLFLEKYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPS 840
F KLFLEKYGYHLDLQKLGYPKLASLLQIMPGVTI ST IVPT K PKVS
Sbjct: 781 AFRKLFLEKYGYHLDLQKLGYPKLASLLQIMPGVTIESTFIVPTGKVPKVS--------- 840
Query: 841 DPEKNTIHVV--ENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEA 900
HVV NSD+ESSD P+KDDDFESTWEELGPA TDC RNE+ESTLS ETAEA
Sbjct: 841 -------HVVANSNSDNESSDLPQKDDDFESTWEELGPAFTDCRSRNEDESTLSSETAEA 900
Query: 901 TKRQPKVDYEPVLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKE 960
T+++PKV YEPV+ EDE TESDGESCPT QRSEE+AKP+T++EES LLQILDSWY +KE
Sbjct: 901 TEKRPKVCYEPVVLEDESSTESDGESCPTTQRSEEQAKPQTNKEESPLLQILDSWYGNKE 960
Query: 961 DHSRKDKSENSEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKE 1020
+SRK+ SENS+EM DC ENSLK+SSL AK+EANTGSFARK RHQKSYSFV DTDENDKE
Sbjct: 961 HNSRKNNSENSDEMNDCFENSLKVSSLTAKNEANTGSFARKLRHQKSYSFVLDTDENDKE 1017
Query: 1021 KLIDGILSTLKKSSESRIHN 1023
KLIDGIL TLKKSSESR+H+
Sbjct: 1021 KLIDGILGTLKKSSESRVHD 1017
BLAST of Spg027204 vs. NCBI nr
Match:
XP_023007313.1 (uncharacterized protein LOC111499848 [Cucurbita maxima])
HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 814/1030 (79.03%), Postives = 884/1030 (85.83%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFS S S SS N+SF SSSSSRR+DEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRALLLHFSH-SASFSSSNYSFPSSSSSRRYDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 YVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+ PGVL SAASIMWHWNALI+GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESPPGVLCSAASIMWHWNALIKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP IP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQASSIRSEEVSEISSDPKPLSIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGN-GTEQ 360
S+DRD+YGYKKFSRFLLSMPHILKLQTNGDGQ IV +VTPR IEPFESSR TS N GTE+
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHILKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDNDGTEE 360
Query: 361 QDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCP 420
QD NLIAKLNNN SS ES SVP++ S ELNAQD+P KV+PS E KS G+ M GEPST P
Sbjct: 361 QDRNLIAKLNNNDSSPESTSVPLVRSSELNAQDRPEKVQPSYELVKSTGEAMGGEPSTWP 420
Query: 421 VSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHNSENGSHHV 480
VSE V+EDSKQTSKFEA +NV PSI QHSE K GF RRIWRRF +GS DHNSENGSHH+
Sbjct: 421 VSESRVLEDSKQTSKFEADNNVIPSIEQHSEAKTGFFRRIWRRFVVGSKDHNSENGSHHI 480
Query: 481 SEKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAK 540
SEKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSV VSNSP+RE AK
Sbjct: 481 SEKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSQDANSVRPVSNSPDREPAK 540
Query: 541 LQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQL 600
LQ+TA+VAS+Y DKS S PG L SIRNWFK + NDTE K SE CCEQNQLK++ KH+L
Sbjct: 541 LQKTAVVASAYDDKSRSRPGALSSIRNWFKIRENDTETGKESEHCCEQNQLKNECGKHRL 600
Query: 601 FSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLI 660
FSS+SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GGPLVLKSLS+S+LFDF+E LI
Sbjct: 601 FSSNSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEGGPLVLKSLSSSELFDFIESLI 660
Query: 661 SDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP----SPEHDSD 720
SDKKW+ ECPSETNPFK+TLSTA SSCTKPLHRANGLTSIFMNKVSQP S EHDSD
Sbjct: 661 SDKKWLVECPSETNPFKVTLSTAETSSCTKPLHRANGLTSIFMNKVSQPSLQGSSEHDSD 720
Query: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLE 780
SDKKNENIPQAGISTTMTKSKFPERTRSEIL DCQ LVD IL EHPEGYNMG F KLFLE
Sbjct: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILCDCQNLVDAILREHPEGYNMGAFRKLFLE 780
Query: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIH 840
KYGYHLDLQKLGYPKLASLLQIMPGVTI ST IVPT K PKVS H
Sbjct: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIESTFIVPTGKVPKVS----------------H 840
Query: 841 VVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYE 900
VV NSD+ESSD PRKDDDFESTWEELGPA TDC RNE+EST S ETAEAT+++PKV YE
Sbjct: 841 VVANSDNESSDLPRKDDDFESTWEELGPAFTDCRSRNEDESTSSSETAEATEKRPKVCYE 900
Query: 901 PVLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSEN 960
PV+ EDE TESDGESCPT QRS E+AKP+T++EES LLQILDSWY +KED S K+KSEN
Sbjct: 901 PVVLEDESSTESDGESCPTTQRSAEQAKPQTNKEESPLLQILDSWYGNKEDISSKNKSEN 960
Query: 961 SEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTL 1020
S+EM C ENSLK+SSL AK+EANTGSFA+K RHQK+YSFV DTDENDKEKLIDGIL TL
Sbjct: 961 SDEMNGCFENSLKVSSLTAKNEANTGSFAQKHRHQKNYSFVLDTDENDKEKLIDGILGTL 1008
Query: 1021 KKSSESRIHN 1023
KKSS+SR+H+
Sbjct: 1021 KKSSKSRVHD 1008
BLAST of Spg027204 vs. NCBI nr
Match:
KAG7011137.1 (Meiosis regulator and mRNA stability factor 1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1493.8 bits (3866), Expect = 0.0e+00
Identity = 805/1030 (78.16%), Postives = 869/1030 (84.37%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSS----ILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHD 60
MR FSS TFSLSSLCSSSSSSS PRVLLL SQFSTSSSS+ RRHD
Sbjct: 2 MRFFSSATFSLSSLCSSSSSSSSSLPFPPRVLLLSLSQFSTSSSSL----------RRHD 61
Query: 61 EESRNVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRAN 120
EESRNVRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGP+QITAFGD+ QLSRAN
Sbjct: 62 EESRNVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPLQITAFGDILQLSRAN 121
Query: 121 QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR 180
QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFA ILHRLR
Sbjct: 122 QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFAGILHRLR 181
Query: 181 MNNYNVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVP 240
MNNYNVLLASP+ APGVL SAASIMWHWN LIRGENL GRHFN+PPDGPYGSWYGHYKVP
Sbjct: 182 MNNYNVLLASPDTAPGVLCSAASIMWHWNTLIRGENLVGRHFNRPPDGPYGSWYGHYKVP 241
Query: 241 LEDPFPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELG 300
LEDP+PVNEQPSSLRA E SELSSDPKPRPIPKTVIRQ+ +ILKL+PKGI ITE RSELG
Sbjct: 242 LEDPYPVNEQPSSLRA-EVSELSSDPKPRPIPKTVIRQLHNILKLHPKGILITELRSELG 301
Query: 301 KSCISMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNG 360
K SMD+DFYGYKKF RFLLS+PHILKLQTNGDGQ IV VTPRPIEP E SR TS +G
Sbjct: 302 K---SMDKDFYGYKKFYRFLLSVPHILKLQTNGDGQCIVCPVTPRPIEPLECSRGTSSDG 361
Query: 361 TEQQDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPS 420
QDPN+ A LNNN SSTES+S VLPS E + +D+ KV+PSSEFG SIG+ MEGE S
Sbjct: 362 NGGQDPNMRANLNNNDSSTESISESVLPSSERSTEDRQLKVKPSSEFGMSIGEGMEGESS 421
Query: 421 TCPVSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHNSENGSH 480
PVSEPHVIEDSKQTS+FEA S+ PSIGQ SE++MGF RRIWRR L +N+H SENGSH
Sbjct: 422 RFPVSEPHVIEDSKQTSQFEAESSKNPSIGQLSESEMGFFRRIWRRLLSNNNHISENGSH 481
Query: 481 HVSEKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESA 540
++SEKCSTSD+ SK S CS LG+ KT KPM DAN VH VSNSP+ ESA
Sbjct: 482 NISEKCSTSDDTSKHKS-----CSG-LLGK----AKTVKPMSQDANCVHPVSNSPDHESA 541
Query: 541 KLQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQ 600
KLQ+TA+VAS Y KSSSNPGLLGSIRNWFKF G +TE +VSE CE+NQLK+QS H
Sbjct: 542 KLQKTAVVASEYDVKSSSNPGLLGSIRNWFKFWGKNTENGEVSERSCERNQLKNQSENHH 601
Query: 601 LFSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELL 660
LFSS+SFWQD+QSF+E+PKG+++ISRSKTRSE+A+NLL GGP VLKSLS SDLFDFLELL
Sbjct: 602 LFSSNSFWQDIQSFMETPKGVQIISRSKTRSEIAENLLEGGPPVLKSLSISDLFDFLELL 661
Query: 661 ISDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVS----QPSPEHDS 720
ISDKKWV ECPSE NPFKLTLS A KSSCTK LH ANGL SIFMNKVS Q S EHDS
Sbjct: 662 ISDKKWVVECPSEANPFKLTLSVARKSSCTKQLHHANGLASIFMNKVSQCSLQGSAEHDS 721
Query: 721 DSDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFL 780
DSDKKNENIPQAG TTMT+ KFPERTRSEILGDC+KLVDEIL +HPEGY MG F KLFL
Sbjct: 722 DSDKKNENIPQAGHYTTMTRRKFPERTRSEILGDCRKLVDEILRDHPEGYKMGAFRKLFL 781
Query: 781 EKYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTI 840
EKYGYHL+LQKLGY KLASLLQIMPGV +AST IVPTSKAPKVS LET SDPEK T
Sbjct: 782 EKYGYHLNLQKLGYHKLASLLQIMPGVAVASTLIVPTSKAPKVSKLETALL-SDPEKKTS 841
Query: 841 HVVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDY 900
HVV S ++SS PRKDDDFES+W ELGPACTD S NE ESTL R+TAEAT+++P VDY
Sbjct: 842 HVVVTSGNDSSVLPRKDDDFESSWGELGPACTDWSNINEAESTLIRDTAEATEKRPMVDY 901
Query: 901 EPVLSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSEN 960
EPVL EDELTESDGESCP RSEE+AK RTDEEESSL+QILDSWYSS+ED SRKDKSEN
Sbjct: 902 EPVLPEDELTESDGESCPATHRSEEQAKQRTDEEESSLIQILDSWYSSEED-SRKDKSEN 961
Query: 961 SEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTL 1020
S+E IDCSENS KLSSLAAKSEANT SFARKQRHQKSYSFVSDTDE D +LIDGI TL
Sbjct: 962 SDERIDCSENSSKLSSLAAKSEANTESFARKQRHQKSYSFVSDTDEKDNVELIDGIFGTL 1005
Query: 1021 KKSSESRIHN 1023
KKSSESRIHN
Sbjct: 1022 KKSSESRIHN 1005
BLAST of Spg027204 vs. ExPASy Swiss-Prot
Match:
E1BP74 (Meiosis regulator and mRNA stability factor 1 OS=Bos taurus OX=9913 GN=MARF1 PE=3 SV=2)
HSP 1 Score: 65.5 bits (158), Expect = 4.1e-09
Identity = 46/152 (30.26%), Postives = 77/152 (50.66%), Query Frame = 0
Query: 64 VSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEA---L 123
+ V+WD ENC+VPSG + A + +R KG + F V +S+ N+E L
Sbjct: 351 IGVFWDIENCSVPSG----RSATAVVQRIREKFFKGHRE-AEFICVCDISKENKEVIQEL 410
Query: 124 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR-MNN 183
++ +++ HI KN+AD L L + + + PA + L+S D +FA L LR +
Sbjct: 411 NNCQVTVAHINATAKNAADDKLRQSLRRFANTHTAPATVVLVSTDVNFALELSDLRHRHG 470
Query: 184 YNVLLASPENAPGVLYSAASIMWHWNALIRGE 212
++++L A + +++ H N LIR E
Sbjct: 471 FHIILVHKNQA------SEALLHHANELIRFE 491
BLAST of Spg027204 vs. ExPASy Swiss-Prot
Match:
Q9Y4F3 (Meiosis regulator and mRNA stability factor 1 OS=Homo sapiens OX=9606 GN=MARF1 PE=1 SV=6)
HSP 1 Score: 65.5 bits (158), Expect = 4.1e-09
Identity = 46/152 (30.26%), Postives = 77/152 (50.66%), Query Frame = 0
Query: 64 VSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEA---L 123
+ V+WD ENC+VPSG + A + +R KG + F V +S+ N+E L
Sbjct: 353 IGVFWDIENCSVPSG----RSATAVVQRIREKFFKGHRE-AEFICVCDISKENKEVIQEL 412
Query: 124 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR-MNN 183
++ +++ HI KN+AD L L + + + PA + L+S D +FA L LR +
Sbjct: 413 NNCQVTVAHINATAKNAADDKLRQSLRRFANTHTAPATVVLVSTDVNFALELSDLRHRHG 472
Query: 184 YNVLLASPENAPGVLYSAASIMWHWNALIRGE 212
++++L A + +++ H N LIR E
Sbjct: 473 FHIILVHKNQA------SEALLHHANELIRFE 493
BLAST of Spg027204 vs. ExPASy Swiss-Prot
Match:
Q8VIG2 (Meiosis regulator and mRNA stability factor 1 OS=Rattus norvegicus OX=10116 GN=Marf1 PE=1 SV=2)
HSP 1 Score: 64.7 bits (156), Expect = 7.0e-09
Identity = 55/201 (27.36%), Postives = 94/201 (46.77%), Query Frame = 0
Query: 64 VSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEA---L 123
+ V+WD ENC+VPSG + A + +R +G + F V +S+ N+E L
Sbjct: 351 IGVFWDIENCSVPSG----RSATTVVQRIREKFFRGHRE-AEFICVCDISKENKEVIQEL 410
Query: 124 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR-MNN 183
++ +++ HI KN+AD L L + + + PA + L+S D +FA L LR +
Sbjct: 411 NNCQVTVAHINATAKNAADDKLRQSLRRFANTHTAPATVVLVSTDVNFALELSDLRHRHG 470
Query: 184 YNVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLED 243
++++L A + +++ H N LIR E + PP P K+P
Sbjct: 471 FHIILVHKNQA------SEALLHHANQLIRFEEFIS---DLPPRLP-------LKIPCHT 527
Query: 244 PFPVNEQPSSLRAKEGSELSS 261
V P++ K+G +S+
Sbjct: 531 LLYVYNLPAN---KDGKSISN 527
BLAST of Spg027204 vs. ExPASy Swiss-Prot
Match:
Q8BJ34 (Meiosis regulator and mRNA stability factor 1 OS=Mus musculus OX=10090 GN=Marf1 PE=1 SV=3)
HSP 1 Score: 64.3 bits (155), Expect = 9.1e-09
Identity = 45/152 (29.61%), Postives = 77/152 (50.66%), Query Frame = 0
Query: 64 VSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEA---L 123
+ V+WD ENC+VPSG + A + +R +G + F V +S+ N+E L
Sbjct: 352 IGVFWDIENCSVPSG----RSATTVVQRIREKFFRGHRE-AEFICVCDISKENKEVIQEL 411
Query: 124 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR-MNN 183
++ +++ HI KN+AD L L + + + PA + L+S D +FA L LR +
Sbjct: 412 NNCQVTVAHINATAKNAADDKLRQSLRRFANTHTAPATVVLVSTDVNFALELSDLRHRHG 471
Query: 184 YNVLLASPENAPGVLYSAASIMWHWNALIRGE 212
++++L A + +++ H N LIR E
Sbjct: 472 FHIILVHKNQA------SEALLHHANQLIRFE 492
BLAST of Spg027204 vs. ExPASy Swiss-Prot
Match:
B2GUN4 (Meiosis regulator and mRNA stability factor 1 OS=Xenopus tropicalis OX=8364 GN=marf1 PE=2 SV=1)
HSP 1 Score: 63.5 bits (153), Expect = 1.5e-08
Identity = 44/149 (29.53%), Postives = 73/149 (48.99%), Query Frame = 0
Query: 64 VSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEA---L 123
+ V+WD ENC+VPSG + A + +R KG + F V +S+ N+E L
Sbjct: 340 IGVFWDIENCSVPSG----RSAVTVVKRIRERLFKGHRE-AEFICVCDISKENKEVIEEL 399
Query: 124 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR-MNN 183
++ +++ HI KN+AD L L + + PA + L+S D +FA L LR ++
Sbjct: 400 NNCQVTVAHINATAKNAADDKLRQSLRRFADTHTSPATVVLVSTDVNFALELSDLRHRHS 459
Query: 184 YNVLLASPENAPGVLYSAASIMWHWNALI 209
++++L A L A + H+ I
Sbjct: 460 FHIILIHKNQASEALLHHAHELIHFEEFI 483
BLAST of Spg027204 vs. ExPASy TrEMBL
Match:
A0A6J1G8G6 (uncharacterized protein LOC111451828 OS=Cucurbita moschata OX=3662 GN=LOC111451828 PE=4 SV=1)
HSP 1 Score: 1577.0 bits (4082), Expect = 0.0e+00
Identity = 826/1029 (80.27%), Postives = 896/1029 (87.07%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFSQFS S SS N+SF SSSSSRRHDEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRTLLLHFSQFSASFSSSNYSFPSSSSSRRHDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 YVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+APGVL SAASIMWHWNAL++GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESAPGVLCSAASIMWHWNALVKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP PIP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQVSSIRSEEVSEISSDPKPLPIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQ 360
S+DRD+YGYKKFSRFLLSMPH LKLQTNGDGQ IV +VTPR IEPFESSR TS NGTE+Q
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHTLKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDNGTEEQ 360
Query: 361 DPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCPV 420
D NLIAKLNNNGSS ES SVP++ S ELNAQD+P KV+PS E KS G+ M GEPST PV
Sbjct: 361 DRNLIAKLNNNGSSPESTSVPLVRSSELNAQDRPEKVQPSYELVKSTGEAMGGEPSTWPV 420
Query: 421 SEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHNSENGSHHVS 480
SEPHV+EDSKQTSKFEA +N+ PSIGQHSE K GF RRIWRRF +GS DHNSENGSHH+S
Sbjct: 421 SEPHVMEDSKQTSKFEADNNMIPSIGQHSEAKTGFFRRIWRRFVVGSKDHNSENGSHHIS 480
Query: 481 EKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAKL 540
EKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSVH VSNS +RE AKL
Sbjct: 481 EKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSQDANSVHPVSNSADREPAKL 540
Query: 541 QRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQLF 600
Q+TA+VAS+Y DKS S PG+L SIRNWFK + NDTE K SE+CCEQNQLK++ KHQLF
Sbjct: 541 QKTAVVASAYDDKSRSRPGVLSSIRNWFKIRENDTETGKESEQCCEQNQLKNECGKHQLF 600
Query: 601 SSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLIS 660
SS SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GPLVLKSLSTS+LFDF+E LIS
Sbjct: 601 SSSSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEEGPLVLKSLSTSELFDFIESLIS 660
Query: 661 DKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP----SPEHDSDS 720
DKKW+ ECPSETNPFK+TLSTA KSSCTKPLHRANGLTSIFMN+VSQP S EHDSDS
Sbjct: 661 DKKWLVECPSETNPFKVTLSTAEKSSCTKPLHRANGLTSIFMNRVSQPSLQGSSEHDSDS 720
Query: 721 DKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLEK 780
DKKNEN+PQAGISTTMTKSKFPERTRSEIL DCQ LVDEIL EHPEGYNMG F KLFLEK
Sbjct: 721 DKKNENVPQAGISTTMTKSKFPERTRSEILCDCQNLVDEILREHPEGYNMGAFRKLFLEK 780
Query: 781 YGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIHV 840
YGYHLDLQKLGYPKLASLLQIMPGV I ST IVPT K PKVS HV
Sbjct: 781 YGYHLDLQKLGYPKLASLLQIMPGVIIESTFIVPTGKVPKVS----------------HV 840
Query: 841 VENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYEP 900
V NSD+ESSD PRKDDDFESTWEELGPA TDC RNE+EST S ETAEAT+++PKV YEP
Sbjct: 841 VANSDNESSDLPRKDDDFESTWEELGPAFTDCRSRNEDESTSSSETAEATEKRPKVCYEP 900
Query: 901 VLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSENS 960
V+ EDE TESDGESCPT QRSEE+AKP+T++EES LLQILDSWY +KED+SRK+KSENS
Sbjct: 901 VVLEDESSTESDGESCPTTQRSEEQAKPQTNKEESPLLQILDSWYGNKEDNSRKNKSENS 960
Query: 961 EEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTLK 1020
+EM DC ENSLK+SSL AK+EANTGSFARK RHQKSYSFV DTDENDKEKLIDGIL TLK
Sbjct: 961 DEMNDCFENSLKVSSLTAKNEANTGSFARKHRHQKSYSFVLDTDENDKEKLIDGILGTLK 1008
Query: 1021 KSSESRIHN 1023
KSSESR+H+
Sbjct: 1021 KSSESRVHD 1008
BLAST of Spg027204 vs. ExPASy TrEMBL
Match:
A0A6J1L7C4 (uncharacterized protein LOC111499848 OS=Cucurbita maxima OX=3661 GN=LOC111499848 PE=4 SV=1)
HSP 1 Score: 1533.9 bits (3970), Expect = 0.0e+00
Identity = 814/1030 (79.03%), Postives = 884/1030 (85.83%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFSS TFSLSSLCSSS PR LLLHFS S S SS N+SF SSSSSRR+DEESR
Sbjct: 1 MRLFSSSTFSLSSLCSSSP-----PRALLLHFSH-SASFSSSNYSFPSSSSSRRYDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
VRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSR NQEAL
Sbjct: 61 YVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRTNQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRSLLVDLM WVSQNPPPAHLFLISGDRDFASILHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSLLVDLMCWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASPE+ PGVL SAASIMWHWNALI+GENL GRHFNQPPDGPYGSWYGHYKVPLEDP
Sbjct: 181 NVLLASPESPPGVLCSAASIMWHWNALIKGENLVGRHFNQPPDGPYGSWYGHYKVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
FPVNEQ SS+R++E SE+SSDPKP IP+ VIR+IR+ILKLYPKGISIT+ RSELGKS I
Sbjct: 241 FPVNEQASSIRSEEVSEISSDPKPLSIPRAVIREIRYILKLYPKGISITDLRSELGKSKI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGN-GTEQ 360
S+DRD+YGYKKFSRFLLSMPHILKLQTNGDGQ IV +VTPR IEPFESSR TS N GTE+
Sbjct: 301 SIDRDYYGYKKFSRFLLSMPHILKLQTNGDGQLIVRIVTPRTIEPFESSRGTSDNDGTEE 360
Query: 361 QDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCP 420
QD NLIAKLNNN SS ES SVP++ S ELNAQD+P KV+PS E KS G+ M GEPST P
Sbjct: 361 QDRNLIAKLNNNDSSPESTSVPLVRSSELNAQDRPEKVQPSYELVKSTGEAMGGEPSTWP 420
Query: 421 VSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRF-LGSNDHNSENGSHHV 480
VSE V+EDSKQTSKFEA +NV PSI QHSE K GF RRIWRRF +GS DHNSENGSHH+
Sbjct: 421 VSESRVLEDSKQTSKFEADNNVIPSIEQHSEAKTGFFRRIWRRFVVGSKDHNSENGSHHI 480
Query: 481 SEKCSTSDNASKQSS-GLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAK 540
SEKCSTSD+ASKQ S G VT SN +LGE K++GKT KPM DANSV VSNSP+RE AK
Sbjct: 481 SEKCSTSDDASKQKSCGHVTNYSNQNLGEAKSEGKTVKPMSQDANSVRPVSNSPDREPAK 540
Query: 541 LQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQL 600
LQ+TA+VAS+Y DKS S PG L SIRNWFK + NDTE K SE CCEQNQLK++ KH+L
Sbjct: 541 LQKTAVVASAYDDKSRSRPGALSSIRNWFKIRENDTETGKESEHCCEQNQLKNECGKHRL 600
Query: 601 FSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLI 660
FSS+SFWQDMQSF+E+PKG+ELI +SKTRSEMAQ LL GGPLVLKSLS+S+LFDF+E LI
Sbjct: 601 FSSNSFWQDMQSFIETPKGVELILQSKTRSEMAQKLLEGGPLVLKSLSSSELFDFIESLI 660
Query: 661 SDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQP----SPEHDSD 720
SDKKW+ ECPSETNPFK+TLSTA SSCTKPLHRANGLTSIFMNKVSQP S EHDSD
Sbjct: 661 SDKKWLVECPSETNPFKVTLSTAETSSCTKPLHRANGLTSIFMNKVSQPSLQGSSEHDSD 720
Query: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLE 780
SDKKNENIPQAGISTTMTKSKFPERTRSEIL DCQ LVD IL EHPEGYNMG F KLFLE
Sbjct: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILCDCQNLVDAILREHPEGYNMGAFRKLFLE 780
Query: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIH 840
KYGYHLDLQKLGYPKLASLLQIMPGVTI ST IVPT K PKVS H
Sbjct: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIESTFIVPTGKVPKVS----------------H 840
Query: 841 VVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYE 900
VV NSD+ESSD PRKDDDFESTWEELGPA TDC RNE+EST S ETAEAT+++PKV YE
Sbjct: 841 VVANSDNESSDLPRKDDDFESTWEELGPAFTDCRSRNEDESTSSSETAEATEKRPKVCYE 900
Query: 901 PVLSEDE-LTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSEN 960
PV+ EDE TESDGESCPT QRS E+AKP+T++EES LLQILDSWY +KED S K+KSEN
Sbjct: 901 PVVLEDESSTESDGESCPTTQRSAEQAKPQTNKEESPLLQILDSWYGNKEDISSKNKSEN 960
Query: 961 SEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTL 1020
S+EM C ENSLK+SSL AK+EANTGSFA+K RHQK+YSFV DTDENDKEKLIDGIL TL
Sbjct: 961 SDEMNGCFENSLKVSSLTAKNEANTGSFAQKHRHQKNYSFVLDTDENDKEKLIDGILGTL 1008
Query: 1021 KKSSESRIHN 1023
KKSS+SR+H+
Sbjct: 1021 KKSSKSRVHD 1008
BLAST of Spg027204 vs. ExPASy TrEMBL
Match:
A0A6J1EQP8 (uncharacterized protein LOC111434983 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434983 PE=4 SV=1)
HSP 1 Score: 1483.4 bits (3839), Expect = 0.0e+00
Identity = 801/1029 (77.84%), Postives = 867/1029 (84.26%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILP---RVLLLHFSQFSTSSSSINHSFHSSSSSRRHDE 60
MRLFSS TFSLSSLCSSSSSSS LP RVLLL SQFSTSSSS+ RRHDE
Sbjct: 2 MRLFSSATFSLSSLCSSSSSSSSLPFPSRVLLLGLSQFSTSSSSL----------RRHDE 61
Query: 61 ESRNVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQ 120
ESRNVRVSVWWDFENCN+P+GVNVFKVAHLITAAVRANGIKGP+QITAFGD+ QLSRANQ
Sbjct: 62 ESRNVRVSVWWDFENCNIPAGVNVFKVAHLITAAVRANGIKGPLQITAFGDILQLSRANQ 121
Query: 121 EALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRM 180
EALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFA ILHRLRM
Sbjct: 122 EALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFAGILHRLRM 181
Query: 181 NNYNVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPL 240
NNYNVLLASP+ APGVL SAASIMWHWN LIRGENL GRHFN+PPDGPYGSWYGHYKVPL
Sbjct: 182 NNYNVLLASPDTAPGVLCSAASIMWHWNTLIRGENLVGRHFNRPPDGPYGSWYGHYKVPL 241
Query: 241 EDPFPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGK 300
EDP+PVNEQPSSLRA E SELSSDPKPRPIPKTVIRQ+ +ILKL+PKGI ITE RSELGK
Sbjct: 242 EDPYPVNEQPSSLRA-EVSELSSDPKPRPIPKTVIRQLHNILKLHPKGILITELRSELGK 301
Query: 301 SCISMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGT 360
SMD+DFYGYKKF RFLLS+PHILKLQTNGDGQ IV VTP PIEP E SR TS +G
Sbjct: 302 ---SMDKDFYGYKKFYRFLLSVPHILKLQTNGDGQCIVCPVTPSPIEPLECSRGTSSDGN 361
Query: 361 EQQDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPST 420
QDPN+ A LNNN SSTES+S VLPS E +A+D+ KV+PSSEFG SIG+ MEGE S
Sbjct: 362 GGQDPNMRANLNNNDSSTESISESVLPSSERSAEDRQLKVKPSSEFGMSIGEGMEGESSR 421
Query: 421 CPVSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHNSENGSHH 480
PVSEPHVIEDSKQTS+FEA S+ PSIGQ SE++MGF RRIWRR L +N+H ENGSH+
Sbjct: 422 FPVSEPHVIEDSKQTSQFEAESSKNPSIGQLSESEMGFFRRIWRRLLSNNNHICENGSHN 481
Query: 481 VSEKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAK 540
+SEKCSTSD+ SK S CS LG+ KT KPM DAN VH VSNSP+ ESAK
Sbjct: 482 ISEKCSTSDDTSKHKS-----CSG-LLGK----AKTVKPMSQDANCVHPVSNSPDHESAK 541
Query: 541 LQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQL 600
LQ+TA+VAS Y K SSNPGLLGSIRNWFKF G +TE +VSE CE+NQLK+QS H L
Sbjct: 542 LQKTAVVASEYDVKFSSNPGLLGSIRNWFKFWGKNTENGEVSERSCERNQLKNQSENHHL 601
Query: 601 FSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLI 660
FSS+SFWQD+QSF+E+PKG+++I RSKTRSE+A+NLL GGP VLKSLS SDLFDFLELLI
Sbjct: 602 FSSNSFWQDIQSFMETPKGVQIILRSKTRSEIAENLLEGGPPVLKSLSISDLFDFLELLI 661
Query: 661 SDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVS----QPSPEHDSD 720
SDKKWV ECPSE NPFKLTLS A KSSCTK LH ANGL SIFMNKVS Q S EHDSD
Sbjct: 662 SDKKWVVECPSEANPFKLTLSVARKSSCTKQLHHANGLASIFMNKVSQCSLQGSAEHDSD 721
Query: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLE 780
S+KKNENIPQAG TTMT+ KFPERTRSEILGDC+KLVDEIL +HPEGY MG F KLFLE
Sbjct: 722 SEKKNENIPQAGHYTTMTRRKFPERTRSEILGDCRKLVDEILRDHPEGYKMGAFRKLFLE 781
Query: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIH 840
KYGYHL+LQKLGY KLASLLQIMPGV +AST IVPTSKAPKVS LET SDP K T H
Sbjct: 782 KYGYHLNLQKLGYHKLASLLQIMPGVAVASTLIVPTSKAPKVSKLETALL-SDPGKKTSH 841
Query: 841 VVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYE 900
VV S ++SS PRKDDDFES+W ELGPACTD S NE ESTL R+TAEAT+++P VDYE
Sbjct: 842 VVVTSGNDSSVLPRKDDDFESSWGELGPACTDWSNINEAESTLIRDTAEATEKRPMVDYE 901
Query: 901 PVLSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSENS 960
PVLSEDELTESDGESCP RSEE+AK RTDEEESSL+QILDSWYSS+ED SRKDK ENS
Sbjct: 902 PVLSEDELTESDGESCPATHRSEEQAKQRTDEEESSLIQILDSWYSSEED-SRKDKLENS 961
Query: 961 EEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILSTLK 1020
+E IDCSENS KLSSLAAKSEANT SFARKQRHQKSYSFVSDTDE D +LIDGI TLK
Sbjct: 962 DERIDCSENSSKLSSLAAKSEANTESFARKQRHQKSYSFVSDTDEKDDVELIDGIFGTLK 1004
Query: 1021 KSSESRIHN 1023
KSS+SRIHN
Sbjct: 1022 KSSKSRIHN 1004
BLAST of Spg027204 vs. ExPASy TrEMBL
Match:
A0A6J1I6A8 (uncharacterized protein LOC111470318 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470318 PE=4 SV=1)
HSP 1 Score: 1469.9 bits (3804), Expect = 0.0e+00
Identity = 800/1031 (77.59%), Postives = 862/1031 (83.61%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSS----ILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHD 60
MRLFSS TFSLSSLCSSSSS S PRVLLL SQF+TSSSS+ RRHD
Sbjct: 3 MRLFSSATFSLSSLCSSSSSYSSSLPFPPRVLLLSLSQFATSSSSL----------RRHD 62
Query: 61 EESRNVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRAN 120
EESRNVRVSVWWDFENCN+P+ VNVFKVAHLITAAVRANGIKGPVQITAFGD+ QLSRAN
Sbjct: 63 EESRNVRVSVWWDFENCNIPAAVNVFKVAHLITAAVRANGIKGPVQITAFGDILQLSRAN 122
Query: 121 QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR 180
QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR
Sbjct: 123 QEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLR 182
Query: 181 MNNYNVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVP 240
MNNYNVLLASP+ APGVL SAASIMWHWN LIRGENL GRHFN+PPDGPYGSWYGHYKVP
Sbjct: 183 MNNYNVLLASPDTAPGVLCSAASIMWHWNTLIRGENLVGRHFNRPPDGPYGSWYGHYKVP 242
Query: 241 LEDPFPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELG 300
LEDP+PVNEQPSSLRA E SELSSDPKPRPIPKTVIRQ+ +ILKL+PKGI IT+ RSELG
Sbjct: 243 LEDPYPVNEQPSSLRA-EVSELSSDPKPRPIPKTVIRQLHNILKLHPKGILITDLRSELG 302
Query: 301 KSCISMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNG 360
K SMD+DFYGYKKF RFLLSMPHILKLQTNGDGQ IV VTP IEP E SR TS +G
Sbjct: 303 K---SMDKDFYGYKKFYRFLLSMPHILKLQTNGDGQCIVRPVTPGSIEPLECSRGTSSDG 362
Query: 361 TEQQDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPS 420
QDPN+ A LNNN SSTES S VLPS E +A+D+ KV+PSSEFG SIG+ MEGE S
Sbjct: 363 NGGQDPNMRANLNNNDSSTESTSESVLPSRERSAEDRQLKVKPSSEFGMSIGEGMEGESS 422
Query: 421 TCPVSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHNSENGSH 480
PVSEPHVIEDSKQTS+FEA S+ PSIGQ SE MGF RRIWRR L +N+H SENGSH
Sbjct: 423 RFPVSEPHVIEDSKQTSQFEAESSKNPSIGQLSEAGMGFFRRIWRRLLSNNNHISENGSH 482
Query: 481 HVSEKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESA 540
++SEKCSTSD+ SK S CS LG+ KT KPM DAN VH VS SP+ ESA
Sbjct: 483 NISEKCSTSDDTSKHKS-----CSG-LLGK----AKTVKPMSQDANCVHPVSISPDHESA 542
Query: 541 KLQRTAIVASSYADKSSSNPGLLGSI-RNWFKFQGNDTEISKVSEECCEQNQLKDQSRKH 600
KLQ+TA+VAS Y KSSSNPGLLGSI RNWFKF G +TE +VSE CE+NQLK+QS H
Sbjct: 543 KLQKTAVVASEYDVKSSSNPGLLGSIRRNWFKFWGKNTENGEVSECSCERNQLKNQSENH 602
Query: 601 QLFSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLEL 660
LFSS+SFWQD+QSF+E+PKG+++ISRSKTRSE+A+NLL GGP VLKSLS SDLFDFLEL
Sbjct: 603 HLFSSNSFWQDIQSFMETPKGVKIISRSKTRSEIAENLLEGGPPVLKSLSISDLFDFLEL 662
Query: 661 LISDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVS----QPSPEHD 720
LISDKKWV ECPSE NPFKLTLS A KSSCTK LH ANGL SIFMNKVS Q S EHD
Sbjct: 663 LISDKKWVVECPSEANPFKLTLSVARKSSCTKQLHHANGLASIFMNKVSQCSLQGSAEHD 722
Query: 721 SDSDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLF 780
SDSDKKNENIPQAG TTMT+ KFPERTRSEILGDC+KLVDEIL +HPEGY MG F KLF
Sbjct: 723 SDSDKKNENIPQAGYYTTMTRRKFPERTRSEILGDCRKLVDEILRDHPEGYKMGAFRKLF 782
Query: 781 LEKYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNT 840
LEKYGYHL+LQKLGY KLASLLQIMPGV +AST IVPTSKAPKVS LET SDPEK T
Sbjct: 783 LEKYGYHLNLQKLGYHKLASLLQIMPGVAVASTLIVPTSKAPKVSKLET-SLLSDPEKKT 842
Query: 841 IHVVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVD 900
H+V SD+ESSD RKDDDFES+WEELGPACTD S NE ESTL +TAEAT ++P VD
Sbjct: 843 SHIVVTSDNESSDLLRKDDDFESSWEELGPACTDWSNINEAESTLISDTAEATVKRPMVD 902
Query: 901 YEPVLSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSE 960
YEPVL EDELTESDGESC RSEE+AK R DEEESSL+QILDSWYS+KED SRK+KSE
Sbjct: 903 YEPVLPEDELTESDGESCHATHRSEEQAKQRPDEEESSLIQILDSWYSNKED-SRKNKSE 962
Query: 961 NSEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFVSDTDENDKEKLIDGILST 1020
N++E IDCSENSLKLSSLAAKSEANT SFARKQRHQKSYSFVSDTDE D +LIDGI
Sbjct: 963 NNDERIDCSENSLKLSSLAAKSEANTESFARKQRHQKSYSFVSDTDEKDDVELIDGIFGM 1007
Query: 1021 LKKSSESRIHN 1023
LKKSSESRIHN
Sbjct: 1023 LKKSSESRIHN 1007
BLAST of Spg027204 vs. ExPASy TrEMBL
Match:
A0A6J1DT12 (uncharacterized protein LOC111024116 OS=Momordica charantia OX=3673 GN=LOC111024116 PE=4 SV=1)
HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 687/973 (70.61%), Postives = 765/973 (78.62%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESR 60
MRLFS GTFS+ SS+S PR LLLHFSQFST+ SS N+SFHSSSSSRR DEESR
Sbjct: 1 MRLFSLGTFSI-------SSASFPPRALLLHFSQFSTAYSSTNYSFHSSSSSRRRDEESR 60
Query: 61 NVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEAL 120
NV+VSVWWDFENCNVP G NVFK AHLITAAVRANGIKGPV ITAFGD+FQLSRANQEAL
Sbjct: 61 NVKVSVWWDFENCNVPVGANVFKAAHLITAAVRANGIKGPVHITAFGDIFQLSRANQEAL 120
Query: 121 SSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNY 180
SSTGISLTHIPQGGKNSADRS L+DLMYWVSQNPPPAH+FLISGDRDFA+ LHRLRMNNY
Sbjct: 121 SSTGISLTHIPQGGKNSADRSFLLDLMYWVSQNPPPAHIFLISGDRDFANTLHRLRMNNY 180
Query: 181 NVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDP 240
NVLLASP +AP VL SAASIMWHWN LIRGENL G+H NQPPDGPYGSWYGHY VPLEDP
Sbjct: 181 NVLLASPASAPSVLCSAASIMWHWNTLIRGENLVGKHLNQPPDGPYGSWYGHYNVPLEDP 240
Query: 241 FPVNEQPSSLRAKEGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCI 300
F +NEQPSS R E SE SSDP PI KTVI+QIR I+KLYPKGI IT+ RSEL S I
Sbjct: 241 FRINEQPSSSRVDEVSEPSSDPNAHPISKTVIKQIRCIMKLYPKGIPITKLRSELRMSNI 300
Query: 301 SMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQ 360
S+ KKF++FLLS+PHI KL+TN DGQ IV L TPR IEPFESSR SGN TE Q
Sbjct: 301 SI-------KKFAQFLLSVPHI-KLRTNSDGQCIVRLATPRAIEPFESSRGISGNDTEDQ 360
Query: 361 DPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCPV 420
PN+IAKLNNNGSS E SVP E NA+D+P KV SSE G SIG+ ME +PS
Sbjct: 361 QPNVIAKLNNNGSSIEGTSVP-----EQNAEDRPMKVEQSSELGMSIGEAMEAKPSI--- 420
Query: 421 SEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHN-SENGSHHVS 480
V+EDSKQTS+ E SN+ SI QHSE + GF RRIW+RFLGS +HN SENGSH++S
Sbjct: 421 ----VVEDSKQTSRVEGDSNMPSSIAQHSEGESGFFRRIWKRFLGSYEHNSSENGSHYIS 480
Query: 481 EKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAKLQ 540
KCSTSD+A K KT+GK+ KPM D + +RE LQ
Sbjct: 481 GKCSTSDDAPKP----------------KTEGKSVKPMTQDVSLA-------DRE--LLQ 540
Query: 541 RTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDTEISKVSEECCEQNQLKDQSRKHQLFS 600
+TAIV+S Y +KSSSNPGLLGSIR F F NDT KVSEEC EQN L+ S KH L S
Sbjct: 541 KTAIVSSLYDNKSSSNPGLLGSIRKLFNFWRNDTNNGKVSEECYEQNPLRTHSGKHLLLS 600
Query: 601 SHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLISD 660
+SFWQ+MQSF+E+P G+EL+SRSKTR EMAQNLL GP LKSLS +DL DFLELLISD
Sbjct: 601 RNSFWQEMQSFMETPNGVELVSRSKTRLEMAQNLLEEGPSALKSLSNNDLLDFLELLISD 660
Query: 661 KKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQPS----PEH--DSD 720
KKWVEECPSET+PFKLTL TA KSSCTKP+H +NGLTSIF+NK S+ + EH DSD
Sbjct: 661 KKWVEECPSETSPFKLTLPTAGKSSCTKPVHHSNGLTSIFLNKGSKRTLHGPQEHDSDSD 720
Query: 721 SDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLE 780
SDKKNENIPQ G+ST+MT++K ERTR+EILGDCQKLVDEILSEHP+GYNMG F +LF+E
Sbjct: 721 SDKKNENIPQVGVSTSMTETKLLERTRTEILGDCQKLVDEILSEHPKGYNMGGFKRLFVE 780
Query: 781 KYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIH 840
KYGYHLD QKLGY KLASLLQI+PGVTIAST+IVPTSKAP VSNLETV PSDPEK + H
Sbjct: 781 KYGYHLDFQKLGYQKLASLLQIIPGVTIASTNIVPTSKAPNVSNLETV-LPSDPEKKSSH 840
Query: 841 VVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYE 900
NSD+ESS+P RKDDDFESTWEELGPACT CS RNEEEST S E EATK++P DY+
Sbjct: 841 AAANSDNESSNPHRKDDDFESTWEELGPACTSCS-RNEEEST-SSEAVEATKKRPNFDYK 900
Query: 901 PVLSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKEDHSRKDKSENS 960
PV+SEDELTESDGESCPT T+EEESSLLQILDSWYSSKE++ RKD+ EN+
Sbjct: 901 PVVSEDELTESDGESCPTTTWRS------TNEEESSLLQILDSWYSSKEENRRKDELENA 912
Query: 961 EEMIDCSENSLKL 967
E IDCSE+SLKL
Sbjct: 961 NETIDCSESSLKL 912
BLAST of Spg027204 vs. TAIR 10
Match:
AT5G09840.1 (Putative endonuclease or glycosyl hydrolase )
HSP 1 Score: 594.3 bits (1531), Expect = 1.8e-169
Identity = 406/1024 (39.65%), Postives = 569/1024 (55.57%), Query Frame = 0
Query: 1 MRLFSSGTFSLSSLCSSSSSSSILPRVLLL-----HFSQFSTSSSS----INHSFHSSSS 60
M+ S + SL SSSS++ R + L H S FST+++S ++ S HS S
Sbjct: 1 MKFGSLPSKSLLFRLSSSSTTQSHRRTITLFITNIHSSPFSTTTTSGSNFVSGSSHSPSR 60
Query: 61 SRRHDEESRNVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQ 120
+ DEESR+VRVSVWWDF +CN+P NV+KVA ITAA+R +GIKGP+ ITAFGDV Q
Sbjct: 61 RPQQDEESRSVRVSVWWDFLSCNLPVDTNVYKVAQSITAAIRNSGIKGPITITAFGDVLQ 120
Query: 121 LSRANQEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASI 180
L R+NQ+ALS+TGISLTH+P GGKNSADRSL+ DLM WVSQNPPPAHL LIS D++FAS+
Sbjct: 121 LPRSNQDALSATGISLTHVPNGGKNSADRSLITDLMCWVSQNPPPAHLLLISSDKEFASV 180
Query: 181 LHRLRMNNYNVLLASPENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYG 240
LHRLRMNNYN+LLAS +APGVL SAASIMW W+ALI+GE + G+HFNQPPDGPY SWYG
Sbjct: 181 LHRLRMNNYNILLASKSSAPGVLCSAASIMWDWDALIKGECVTGKHFNQPPDGPYNSWYG 240
Query: 241 HYKVPLEDPFPV----NEQPSSLRAKEGSE---LSSDPKPRPIPKTVIRQIRHILKLYPK 300
HY++PL DPF + + SS++ +E SE ++ RPIPK V+ +IR I+ LYPK
Sbjct: 241 HYRIPLLDPFAIATTTEQSSSSVKIEELSESVNSNAVVNLRPIPKEVVDKIRSIVSLYPK 300
Query: 301 GISITEFRSELGKSCISMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVT-PRPI 360
G +ITE R+EL KS +++D+DFYG+KKFS+FLLSMP IL++ T +G F++ VT +P
Sbjct: 301 GAAITELRAELSKSNLAIDKDFYGHKKFSKFLLSMPDILQVTTVSEGLFMIRAVTEKKPP 360
Query: 361 EPFESSRDTSGNGTEQQDPNLIAKLNNNGSSTESLSVP---VLPSGELNAQDKPSKVRPS 420
+SS P L ++ E+++ P ++ EL A+ R
Sbjct: 361 MRLDSS------------PRLSTAVDQKIKDKETVNAPSPKLISDVELAAER-----RRD 420
Query: 421 SEFGKSIGKTMEG-----EPSTCPVSEPHVI--EDSKQTSKFEAVSNVTPSIGQHSETKM 480
GK K +E E S+ +P ++ +D K K + V S +
Sbjct: 421 GLLGKKQEKVLESDKIVKEESSESSQDPILVGQKDVKANVKPVETNQVALVAWSDSSMED 480
Query: 481 GFLRRIWRRFLGSNDHNSENGSHHVSEKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKT 540
GF +++ R + GS + E H+ E S S + S+ G + K
Sbjct: 481 GFFQKLKRLWYGSPEMELE----HLPEMKSVSGSGDTHREDNDLKSSSQGSGNKHKEDKD 540
Query: 541 EKPMILDANSVHQVSNSPERESAKLQRTAIVASSYADKSSSNPGLLGSIRNWFKFQGNDT 600
K + Q+S S ES + DK +S PG LG + FKF G +T
Sbjct: 541 LKSSSQGTVPMSQISPSFVAESVDEVKVGADDVDSKDKDAS-PGFLGRLLKSFKFWGKNT 600
Query: 601 EISKVSEECCEQNQLKDQSRKHQLFSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNL 660
+ SK S Q + S+ +F+ FW D++SF+ SP+G ++S S+TR MA+N+
Sbjct: 601 KSSKDSSG--NQELVNVDSQVQDIFAKEYFWSDIESFINSPRGFAIVSHSRTREVMAKNV 660
Query: 661 LAGGPLVLKSLSTSDLFDFLELLISDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRAN 720
GP L+ L S + + LLIS+KKW+EE PS + PF++ K S H +N
Sbjct: 661 QEEGPSCLRLLDESSMLHLVTLLISEKKWIEETPSSSLPFRII-----KGSSPGHRHASN 720
Query: 721 GLTSIFMNKVSQPSPEHDSDSDKKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDE 780
GL+SIF + S S + +K +N+ AG+S K ER +S + DCQK++ +
Sbjct: 721 GLSSIFSD--SSKSQWQKQNGEKSGKNVAHAGVSVGSVDRKELERYKSNAIADCQKMIKK 780
Query: 781 ILSEHPEGYNMGTFGKLFLEKYGYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAP 840
I EHPEGY++ F K FLE+YGYHL + KLGY L SL+++M GV IAS +I P++ +P
Sbjct: 781 ITEEHPEGYSLIRFRKDFLEEYGYHLAVDKLGYDNLQSLIRVMHGVRIASGYIFPSTPSP 840
Query: 841 KVSNLETVQFPSDPEKNTIHVVENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEE 900
+ K+DD + + ELGP +
Sbjct: 841 NAKS------------------------------KEDDSDLAFAELGPV---------SD 900
Query: 901 STLSRETAEATKRQPKVDYEPVLSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQI 960
+T + T TK+ P YEP LSEDE E G ++ +++ + + +ESSLLQI
Sbjct: 901 TTTTHPT---TKKLPV--YEPSLSEDE--EDSGSERDNPEKKKQQMMSK-EGKESSLLQI 923
Query: 961 LDSWYSSKEDHSRKDKSENSEEMIDCSENSLKLSSLAAKSEANTGSFARKQRHQKSYSFV 998
LDS+Y++K + LK + + K +N RKQ+ K+YSFV
Sbjct: 961 LDSYYTNK-------------------DGELKENPVERKLVSN----GRKQKPTKTYSFV 923
BLAST of Spg027204 vs. TAIR 10
Match:
AT5G64710.1 (Putative endonuclease or glycosyl hydrolase )
HSP 1 Score: 522.3 bits (1344), Expect = 8.7e-148
Identity = 370/947 (39.07%), Postives = 511/947 (53.96%), Query Frame = 0
Query: 4 FSSGTFSLSSLCSSSSSSSILP-RVLLLHFSQFSTSSSSINHSFHSSSSSRRHDEESRNV 63
F S + SS+S P R + + FS+SSS H ++S +++E+SR+V
Sbjct: 3 FGSSKTLFFRVAGFSSTSVFKPRRAISVPVGNFSSSSSISAH--YTSLKRSQYEEDSRSV 62
Query: 64 RVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEALSS 123
RV VWWDFENC++PSG NVFK+A IT+AVR GIKGP+ ITA+GD+ QLSR NQEAL +
Sbjct: 63 RVPVWWDFENCHLPSGANVFKLAQTITSAVRICGIKGPITITAYGDMIQLSRTNQEALFA 122
Query: 124 TGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNYNV 183
TGI+LTH+PQGGKNS DRSL+ ++M WVSQNPPPAHLFLIS D DFA++LHRLRM NYN+
Sbjct: 123 TGINLTHVPQGGKNSTDRSLITEIMCWVSQNPPPAHLFLISSDSDFANVLHRLRMRNYNI 182
Query: 184 LLAS-PENAPGVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDPF 243
LLA E GVL SAASIMW W+AL+RG+N +HFNQPPDGPY SWYGHY PL DPF
Sbjct: 183 LLACYEETTLGVLCSAASIMWDWDALVRGQNPTAKHFNQPPDGPYHSWYGHYTTPLLDPF 242
Query: 244 PV---NEQPSSLRAK--EGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELG 303
N+Q SS K E EL S RPIP V++QI IL+ YPKG +ITE R +L
Sbjct: 243 ATSTNNKQISSTSVKTVELLELGSSKSRRPIPNKVVKQIGLILRWYPKGAAITELREQLR 302
Query: 304 KSCISMDRDFYGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNG 363
K + +DRDFYGYK FSRFLLSM +IL++ GDG F +H V T G
Sbjct: 303 KRKVLLDRDFYGYKSFSRFLLSMRNILQVVPLGDGMFSIHAV-------------TQGMD 362
Query: 364 TEQQDPNLIAKLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPS 423
+ P + + N+ S E + + + + +++ +++ SS+ + K M+ +
Sbjct: 363 NKALLPKVSCE-NHAVVSVEKM-CQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAK 422
Query: 424 TCPVSEPHVIEDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHNSENGSH 483
PV + AV +V+ E K GFL+++ R GS + E
Sbjct: 423 EEPVKANQL--------AITAVDDVS-----SFEEKDGFLKKLNRLLFGSPEMELE---- 482
Query: 484 HVSEKCSTSDNASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESA 543
H+ E+ K SG N LGE K ++D + Q+++S ESA
Sbjct: 483 HLQER--------KHISG------NGVLGEGK---------VVDKDLESQIASSTSSESA 542
Query: 544 KLQRTAIVASSYADKSSSNPGLLGSIRNWFKFQ-GNDTEISKVSEECCEQNQLKDQSRKH 603
+ + V ++ + S +PGL + FKF G TE+S N +
Sbjct: 543 EEVK---VDNAVGNGKSKSPGLTCRLLKRFKFSWGRYTELS---------NAAATGPQVD 602
Query: 604 QLFSSHSFWQDMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLEL 663
+F SFW D++SF+ SP+G +S S++R MA+NL GP LK L + D + +
Sbjct: 603 DVFVKDSFWNDVESFINSPRGFVAVSHSRSRETMAKNLKEEGPSSLKPLDVPKMLDLISM 662
Query: 664 LISDKKWVEECPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQPSPEHDSDSD 723
LIS+KKW++E PS+ PF++T T +SSC +GL +IF+N E D
Sbjct: 663 LISEKKWIQENPSDALPFRVTRFT-EESSCRSNPRTTDGLRAIFVN-----MSESLCDGA 722
Query: 724 KKNENIPQAGISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLEKY 783
++N G+S K ER+RS+++ DC KL+ +I E+ GY++ F K FLEK+
Sbjct: 723 NGDKNSTNVGMS-----QKPKERSRSKVIADCHKLIKKITEENAGGYSITKFKKAFLEKF 782
Query: 784 GYHLDLQKLGYPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIHVV 843
GY L+ +K G+ KL SL+++MP I S HIV TS P V SD
Sbjct: 783 GYRLEYRKFGFSKLQSLIEMMPEARIESGHIV-TSSTP-------VPCESD--------- 811
Query: 844 ENSDSESSDPPRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYEPV 903
S++E+LGP +E ES+
Sbjct: 843 ------------------SSFEDLGPVSKKI---HENESS-------------------- 811
Query: 904 LSEDELTESDGESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKE 943
+SE E +S+ E + ++S E K + DE ES LLQIL SW + K+
Sbjct: 903 VSEGEDYDSEMEEKASSKQSGGERKKKEDETESDLLQILGSWDTDKK 811
BLAST of Spg027204 vs. TAIR 10
Match:
AT5G64710.2 (Putative endonuclease or glycosyl hydrolase )
HSP 1 Score: 407.9 bits (1047), Expect = 2.4e-113
Identity = 302/817 (36.96%), Postives = 421/817 (51.53%), Query Frame = 0
Query: 133 GGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNYNVLLAS-PENAP 192
GGKNS DRSL+ ++M WVSQNPPPAHLFLIS D DFA++LHRLRM NYN+LLA E
Sbjct: 6 GGKNSTDRSLITEIMCWVSQNPPPAHLFLISSDSDFANVLHRLRMRNYNILLACYEETTL 65
Query: 193 GVLYSAASIMWHWNALIRGENLAGRHFNQPPDGPYGSWYGHYKVPLEDPFPV---NEQPS 252
GVL SAASIMW W+AL+RG+N +HFNQPPDGPY SWYGHY PL DPF N+Q S
Sbjct: 66 GVLCSAASIMWDWDALVRGQNPTAKHFNQPPDGPYHSWYGHYTTPLLDPFATSTNNKQIS 125
Query: 253 SLRAK--EGSELSSDPKPRPIPKTVIRQIRHILKLYPKGISITEFRSELGKSCISMDRDF 312
S K E EL S RPIP V++QI IL+ YPKG +ITE R +L K + +DRDF
Sbjct: 126 STSVKTVELLELGSSKSRRPIPNKVVKQIGLILRWYPKGAAITELREQLRKRKVLLDRDF 185
Query: 313 YGYKKFSRFLLSMPHILKLQTNGDGQFIVHLVTPRPIEPFESSRDTSGNGTEQQDPNLIA 372
YGYK FSRFLLSM +IL++ GDG F +H V T G + P +
Sbjct: 186 YGYKSFSRFLLSMRNILQVVPLGDGMFSIHAV-------------TQGMDNKALLPKVSC 245
Query: 373 KLNNNGSSTESLSVPVLPSGELNAQDKPSKVRPSSEFGKSIGKTMEGEPSTCPVSEPHVI 432
+ N+ S E + + + + +++ +++ SS+ + K M+ + PV +
Sbjct: 246 E-NHAVVSVEKM-CQNMKQNDKDVKEESHQLQESSQEFVQVMKLMDVKAKEEPVKANQL- 305
Query: 433 EDSKQTSKFEAVSNVTPSIGQHSETKMGFLRRIWRRFLGSNDHNSENGSHHVSEKCSTSD 492
AV +V+ E K GFL+++ R GS + E H+ E+
Sbjct: 306 -------AITAVDDVS-----SFEEKDGFLKKLNRLLFGSPEMELE----HLQER----- 365
Query: 493 NASKQSSGLVTTCSNDSLGEVKTDGKTEKPMILDANSVHQVSNSPERESAKLQRTAIVAS 552
K SG N LGE K ++D + Q+++S ESA+ + V +
Sbjct: 366 ---KHISG------NGVLGEGK---------VVDKDLESQIASSTSSESAEEVK---VDN 425
Query: 553 SYADKSSSNPGLLGSIRNWFKFQ-GNDTEISKVSEECCEQNQLKDQSRKHQLFSSHSFWQ 612
+ + S +PGL + FKF G TE+S N + +F SFW
Sbjct: 426 AVGNGKSKSPGLTCRLLKRFKFSWGRYTELS---------NAAATGPQVDDVFVKDSFWN 485
Query: 613 DMQSFLESPKGIELISRSKTRSEMAQNLLAGGPLVLKSLSTSDLFDFLELLISDKKWVEE 672
D++SF+ SP+G +S S++R MA+NL GP LK L + D + +LIS+KKW++E
Sbjct: 486 DVESFINSPRGFVAVSHSRSRETMAKNLKEEGPSSLKPLDVPKMLDLISMLISEKKWIQE 545
Query: 673 CPSETNPFKLTLSTASKSSCTKPLHRANGLTSIFMNKVSQPSPEHDSDSDKKNENIPQAG 732
PS+ PF++T T +SSC +GL +IF+N E D ++N G
Sbjct: 546 NPSDALPFRVTRFT-EESSCRSNPRTTDGLRAIFVN-----MSESLCDGANGDKNSTNVG 605
Query: 733 ISTTMTKSKFPERTRSEILGDCQKLVDEILSEHPEGYNMGTFGKLFLEKYGYHLDLQKLG 792
+S K ER+RS+++ DC KL+ +I E+ GY++ F K FLEK+GY L+ +K G
Sbjct: 606 MS-----QKPKERSRSKVIADCHKLIKKITEENAGGYSITKFKKAFLEKFGYRLEYRKFG 665
Query: 793 YPKLASLLQIMPGVTIASTHIVPTSKAPKVSNLETVQFPSDPEKNTIHVVENSDSESSDP 852
+ KL SL+++MP I S HIV TS P V SD
Sbjct: 666 FSKLQSLIEMMPEARIESGHIV-TSSTP-------VPCESD------------------- 686
Query: 853 PRKDDDFESTWEELGPACTDCSIRNEEESTLSRETAEATKRQPKVDYEPVLSEDELTESD 912
S++E+LGP +E ES+ +SE E +S+
Sbjct: 726 --------SSFEDLGPVSKKI---HENESS--------------------VSEGEDYDSE 686
Query: 913 GESCPTIQRSEEEAKPRTDEEESSLLQILDSWYSSKE 943
E + ++S E K + DE ES LLQIL SW + K+
Sbjct: 786 MEEKASSKQSGGERKKKEDETESDLLQILGSWDTDKK 686
BLAST of Spg027204 vs. TAIR 10
Match:
AT3G62210.1 (Putative endonuclease or glycosyl hydrolase )
HSP 1 Score: 156.0 bits (393), Expect = 1.6e-37
Identity = 78/212 (36.79%), Postives = 116/212 (54.72%), Query Frame = 0
Query: 63 RVSVWWDFENCNVPSGVNVFKVAHLITAAVRANGIKGPVQITAFGDVFQLSRANQEALSS 122
+ SVWWD ENC VP G++ +A I++A++ G V I+A+GD + Q AL+S
Sbjct: 23 KTSVWWDIENCQVPKGLDAHGIAQNISSALKKMNYCGRVSISAYGDTSGIPHVIQHALNS 82
Query: 123 TGISLTHIPQGGKNSADRSLLVDLMYWVSQNPPPAHLFLISGDRDFASILHRLRMNNYNV 182
TGI L H+P G K+++D+ +LVD+++W NP P+++ LISGDRDF++ LH+L + YN+
Sbjct: 83 TGIELHHVPAGVKDASDKKILVDMLFWAFDNPAPSNIMLISGDRDFSNALHKLSLRRYNI 142
Query: 183 LLASPENAPGVLYSAASIMWHWNALIRGEN----------------------LAGRHFNQ 242
LLA P A L AA+ +W W +L+ G N ++ NQ
Sbjct: 143 LLAHPPKASAPLSQAATTVWLWTSLLAGGNPLIRGKVKTSQLVANASTSSNVMSSPPHNQ 202
Query: 243 PPDGP-YGSWYGHYKVPLEDPFPVNEQPSSLR 252
PD P G + DPF N P++ R
Sbjct: 203 FPDPPRSGPLHARQPYLNPDPFVNNRDPNAAR 234
BLAST of Spg027204 vs. TAIR 10
Match:
AT3G62200.1 (Putative endonuclease or glycosyl hydrolase )
HSP 1 Score: 154.8 bits (390), Expect = 3.6e-37
Identity = 76/175 (43.43%), Postives = 110/175 (62.86%), Query Frame = 0
Query: 36 STSSSSINHSFHSSSSSRRHDEESRNVRVSVWWDFENCNVPSGVNVFKVAHLITAAVRAN 95
ST+S+S +S + + + + SVWWD ENC VP+G++ +A IT+A++
Sbjct: 2 STNSASDGDFGTNSVPAEMAEAQYVRAKTSVWWDIENCQVPNGLDAHGIAQNITSALQKM 61
Query: 96 GIKGPVQITAFGDVFQLSRANQEALSSTGISLTHIPQGGKNSADRSLLVDLMYWVSQNPP 155
GPV I+A+GD ++ Q AL+STGI+L H+P G K+++D+ +LVD+++W NP
Sbjct: 62 NYCGPVSISAYGDTNRIPLTIQHALNSTGIALNHVPAGVKDASDKKILVDMLFWALDNPA 121
Query: 156 PAHLFLISGDRDFASILHRLRMNNYNVLLASPENAPGVLYSAASIMWHWNALIRG 211
PA+ LISGDRDF++ LH LRM YNVLLA P A L AA +W W +L G
Sbjct: 122 PANFMLISGDRDFSNALHGLRMRRYNVLLAQPLKASVPLVHAAKTVWLWTSLSAG 176
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| KAG6605277.1 | 0.0e+00 | 80.27 | Meiosis regulator and mRNA stability factor 1, partial [Cucurbita argyrosperma s... | [more] |
| XP_022948172.1 | 0.0e+00 | 80.27 | uncharacterized protein LOC111451828 [Cucurbita moschata] | [more] |
| XP_023534760.1 | 0.0e+00 | 79.04 | uncharacterized protein LOC111796232 [Cucurbita pepo subsp. pepo] | [more] |
| XP_023007313.1 | 0.0e+00 | 79.03 | uncharacterized protein LOC111499848 [Cucurbita maxima] | [more] |
| KAG7011137.1 | 0.0e+00 | 78.16 | Meiosis regulator and mRNA stability factor 1, partial [Cucurbita argyrosperma s... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| E1BP74 | 4.1e-09 | 30.26 | Meiosis regulator and mRNA stability factor 1 OS=Bos taurus OX=9913 GN=MARF1 PE=... | [more] |
| Q9Y4F3 | 4.1e-09 | 30.26 | Meiosis regulator and mRNA stability factor 1 OS=Homo sapiens OX=9606 GN=MARF1 P... | [more] |
| Q8VIG2 | 7.0e-09 | 27.36 | Meiosis regulator and mRNA stability factor 1 OS=Rattus norvegicus OX=10116 GN=M... | [more] |
| Q8BJ34 | 9.1e-09 | 29.61 | Meiosis regulator and mRNA stability factor 1 OS=Mus musculus OX=10090 GN=Marf1 ... | [more] |
| B2GUN4 | 1.5e-08 | 29.53 | Meiosis regulator and mRNA stability factor 1 OS=Xenopus tropicalis OX=8364 GN=m... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1G8G6 | 0.0e+00 | 80.27 | uncharacterized protein LOC111451828 OS=Cucurbita moschata OX=3662 GN=LOC1114518... | [more] |
| A0A6J1L7C4 | 0.0e+00 | 79.03 | uncharacterized protein LOC111499848 OS=Cucurbita maxima OX=3661 GN=LOC111499848... | [more] |
| A0A6J1EQP8 | 0.0e+00 | 77.84 | uncharacterized protein LOC111434983 isoform X1 OS=Cucurbita moschata OX=3662 GN... | [more] |
| A0A6J1I6A8 | 0.0e+00 | 77.59 | uncharacterized protein LOC111470318 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... | [more] |
| A0A6J1DT12 | 0.0e+00 | 70.61 | uncharacterized protein LOC111024116 OS=Momordica charantia OX=3673 GN=LOC111024... | [more] |
