Spg027087 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg027087 vs. NCBI nr
Match: XP_038895093.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 985.7 bits (2547), Expect = 1.5e-283
Identity = 476/533 (89.31%), Postives = 509/533 (95.50%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M ILRSSILLLS S L+FF+SAT APENF QCLL  SPPS+PIAPAIFTPNN S+SSVL+
Sbjct: 1   MEILRSSILLLSFSYLVFFSSATSAPENFFQCLLRHSPPSYPIAPAIFTPNNVSFSSVLE 60

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 61  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 120

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           +PFF+LDMFNLR ITVDVKT+TAWVQTGATLGEVYYRIAEK+KF+GFPAGVCPTVGVGGH
Sbjct: 121 LPFFILDMFNLRDITVDVKTKTAWVQTGATLGEVYYRIAEKNKFYGFPAGVCPTVGVGGH 180

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 240

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIR VRVPETVTVFRV RTLEQND+TEIVDEWQRVAHVIDNDLFIRVTFDV+NGT+ GKK
Sbjct: 241 KIRMVRVPETVTVFRVQRTLEQNDLTEIVDEWQRVAHVIDNDLFIRVTFDVINGTEKGKK 300

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA F+A+FLGDSERLLSV++KSFPKLGLKKSDCLEMSWL+SVLFWT+FPLGTSVD LL
Sbjct: 301 TLRATFLAMFLGDSERLLSVIKKSFPKLGLKKSDCLEMSWLQSVLFWTNFPLGTSVDALL 360

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+ PIPKEGLN+IWKKMIEL KPMLTFNPYGGRMAEIPSNATPF
Sbjct: 361 SRTPQVLTHLKRKSDYVKTPIPKEGLNKIWKKMIELEKPMLTFNPYGGRMAEIPSNATPF 420

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+E+GT++A HFIDLTRKLY FMTPFVSKNPRTAFLNYRDLDIG+
Sbjct: 421 PHRAGNLWKIQYATNWDEEGTQEAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIGV 480

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK SYYEGRV+G+KYF+GNFERLVKIKT+VDP+NFFRNEQSIPRFPHVGR
Sbjct: 481 NHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPDNFFRNEQSIPRFPHVGR 533

BLAST of Spg027087 vs. NCBI nr
Match: KAG6573419.1 (Berberine bridge enzyme-like 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 978.0 bits (2527), Expect = 3.2e-281
Identity = 479/533 (89.87%), Postives = 503/533 (94.37%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M ILR S  LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MEILRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKTETAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG ++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGRRSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKKSDCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Spg027087 vs. NCBI nr
Match: XP_022994779.1 (berberine bridge enzyme-like 8 [Cucurbita maxima])

HSP 1 Score: 976.5 bits (2523), Expect = 9.4e-281
Identity = 478/533 (89.68%), Postives = 504/533 (94.56%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M I R SI LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MEISRFSIFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKTETAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDV G LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG+++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKKSDCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP+NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYVGR 535

BLAST of Spg027087 vs. NCBI nr
Match: XP_022954545.1 (berberine bridge enzyme-like 8 [Cucurbita moschata])

HSP 1 Score: 975.7 bits (2521), Expect = 1.6e-280
Identity = 477/533 (89.49%), Postives = 503/533 (94.37%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M I R S  LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MDISRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKTETAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG+++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKK+DCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Spg027087 vs. NCBI nr
Match: XP_023542409.1 (berberine bridge enzyme-like 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.8 bits (2503), Expect = 2.0e-278
Identity = 474/529 (89.60%), Postives = 500/529 (94.52%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M I R S  LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MEISRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKT+TAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTKTAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG+++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSKSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKKSDCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFP 530
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP+NFFRNEQSIPRFP
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFP 531

BLAST of Spg027087 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 3.4e-193
Identity = 332/513 (64.72%), Postives = 397/513 (77.39%), Query Frame = 0

Query: 20  ASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQTYIRNLRFNTSTTPKPFLI 79
           +S++   E F QCL   S P HPI+PAIF   N SYSSVLQ  IRNLRFNT++TPKPFLI
Sbjct: 19  SSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLI 78

Query: 80  VTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFVLDMFNLRAITVDV 139
           + A HESHV+  I C + HNLQMKIRSGGHDY+G+SYV+    PFFVLDMFNLR++ VDV
Sbjct: 79  IAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDV 138

Query: 140 KTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGYGNMMRKYGLSVD 199
            ++TAWVQTGA LGEVYY I EKSK   +PAG+CPTVGVGGH  GGGYGNMMRKYGL+VD
Sbjct: 139 ASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVD 198

Query: 200 QIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVRVPETVTVFRVGR 259
             IDA++VDVNGK+LDRK MGEDL+WAI GGGG S+ VV AYKI  V VPE VTVFR+ R
Sbjct: 199 NTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISR 258

Query: 260 TLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARFIALFLGDSERLL 319
           TLEQN  T+I+  WQ+VA  + ++LFIR   DVVNGT + +KT+R  FIA+FLGD+  LL
Sbjct: 259 TLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLL 318

Query: 320 SVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELLSRKPQVLTHLKRKSDYVR 379
           S++ + FP+LGL +SDC E SW++SVLFWT+  +G+S + LL ++ Q + +LKRKSDYVR
Sbjct: 319 SILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQPVNYLKRKSDYVR 378

Query: 380 APIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRAGNLWKIQYATNWNE 439
            PI + GL  IWKKMIEL  P + FNPYGG M  I S  TPFP+RAGNLWKIQY  NW +
Sbjct: 379 EPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD 438

Query: 440 QGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGIN-HNGK-KSYYEGRVFGL 499
           +     + +++LTRKLY FMTPFVSKNPR +F NYRD+D+GIN HNGK  SY EG+ +G 
Sbjct: 439 E--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGK 498

Query: 500 KYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFP 530
           KYF GNFERLVKIKTRVD  NFFRNEQSIP  P
Sbjct: 499 KYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Spg027087 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 6.3e-163
Identity = 293/522 (56.13%), Postives = 374/522 (71.65%), Query Frame = 0

Query: 15  SLLFFASATIAP-------ENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQTYIRNL 74
           SL FF+SA  AP       E+F+QC   ++  P   I   +F+  N S+SSVL+ YIRN 
Sbjct: 19  SLPFFSSA--APPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNG 78

Query: 75  RFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 134
           RFNTS+TPKP +IVT   + HV   + C++  N  +KIRSGGHDYEG+SY+SD  PFF+L
Sbjct: 79  RFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFIL 138

Query: 135 DMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGY 194
           DM NLR ++VD+  ++AW+  GATLGEVYYRI EKSK HGFPAGVCPTVGVGGH  GGGY
Sbjct: 139 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 198

Query: 195 GNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVR 254
           GNM+RK+GLSVD +IDAKIVDVNG++LDRK+MGEDLFWAI+GGGG+SF VV  YK++ V 
Sbjct: 199 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 258

Query: 255 VPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARF 314
           VPETVTVFRV + ++   V ++V +WQ V    D +LF+R+    V  T+   KT+RA  
Sbjct: 259 VPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATV 318

Query: 315 IALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDE--LLSRKP 374
           +ALFLG +E +++++ K FP+L LKK +C EM+W +S L+W +    T +D    L R  
Sbjct: 319 VALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNL 378

Query: 375 QVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRA 434
                 KRKSDYV + IP++G+  ++KKM ELGK  L FNPYGG+MAE+  NATPFPHR+
Sbjct: 379 DRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS 438

Query: 435 GNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNG 494
             L+KIQY+  W E   E    F++    LY FMT FVSKNPR A+LNYRD+DIG+N +G
Sbjct: 439 -KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHG 498

Query: 495 KKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIP 527
             SY EG V+G KYF  NF+RLVK+KT  DP+NFFRNEQSIP
Sbjct: 499 TNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Spg027087 vs. ExPASy Swiss-Prot
Match: Q9SA99 (Berberine bridge enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11770 PE=2 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 2.4e-162
Identity = 290/530 (54.72%), Postives = 375/530 (70.75%), Query Frame = 0

Query: 8   ILLLSISSLLFFASATIAP-----ENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQT 67
           ++L  ISS +  + A   P     +NFLQC  +++  P + +A  +     A+++ VL+ 
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+  ++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHF 187
           PFFVLDM NLR ITVD  TE+AWV  GATLGEVYYRI EK+K HGFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYK 247
            GGGYGNM+RKYGLSVD + DAKIVDVNG++LDRK MGED+FWAI GGGG+SF V+ A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKT 307
           I+ V VP TVTVFRV + L +N  TE+V +WQ VA   D  LF+R+    V  T+N  +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSV--DEL 367
           +RA  +ALFLGD   ++S++ K FP+LGLKK +C EM+W++SV++W +    T +  + L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V   I K+GL+ ++KKMIE+GK  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + GTE    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 INHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFP 530
           +N  G  SY    VFG  YF  NF+RLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Spg027087 vs. ExPASy Swiss-Prot
Match: Q9SVG4 (Berberine bridge enzyme-like 19 OS=Arabidopsis thaliana OX=3702 GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 564.7 bits (1454), Expect = 1.1e-159
Identity = 285/522 (54.60%), Postives = 371/522 (71.07%), Query Frame = 0

Query: 8   ILLLSISSLLFFASATIAPENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQTYIRNL 67
           +L LS+  L  F+ +     +FL+C   ++  P   I   +F+  N ++SSVL+ YIRN 
Sbjct: 19  LLFLSL-PLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 78

Query: 68  RFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 127
           RFNTS+T KP +I+T   ESHV   + C++  N  +KIRSGGHDY+G+SY+SD  PFF+L
Sbjct: 79  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFIL 138

Query: 128 DMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGY 187
           DM N+R ++VD+ + +AW+  GATLGEVYYRI EKS+ HGFPAGVCPTVGVGGH  GGGY
Sbjct: 139 DMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGY 198

Query: 188 GNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVR 247
           GNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAITGGGG S+ VV  YK++ V 
Sbjct: 199 GNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVP 258

Query: 248 VPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARF 307
           VP  VTVFRV + ++   V ++V +WQ V    D +LF+R+    V  T+   KT+RA  
Sbjct: 259 VPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASV 318

Query: 308 IALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDE--LLSRKP 367
           +ALFLG ++ +++++ K FP+LGLKK +C EM+W +S L+W +    T VD    L R  
Sbjct: 319 VALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNL 378

Query: 368 QVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRA 427
              +  KRKSDYV   IPK+G+  ++KKMIELGK  L FNPYGG+MAE+  NA PFPHR 
Sbjct: 379 DTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR- 438

Query: 428 GNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNG 487
             L+KIQY+ NW E   E    +++  + LY FMT FVSKNPR+++ NYRD+DIG+N +G
Sbjct: 439 NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHG 498

Query: 488 KKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIP 527
             SY EG V+G KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 ANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Spg027087 vs. ExPASy Swiss-Prot
Match: F4HV09 (Berberine bridge enzyme-like 14 OS=Arabidopsis thaliana OX=3702 GN=At1g34575 PE=3 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-157
Identity = 283/519 (54.53%), Postives = 370/519 (71.29%), Query Frame = 0

Query: 19  FASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQTYIRNLRFNTSTTPKPFL 78
           FA+     ++F QC+     PS PI    +T  N ++ ++L  Y+RNLR+   TT KP  
Sbjct: 22  FAAPPKLKDSFTQCVT-VFKPSVPIQNFTYTQQNPNFLTILNNYVRNLRYFNGTTRKPVA 81

Query: 79  IVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVLDMFNLRAITVDV 138
           IV A H +H++  I CA+   LQ++IRSGGHDY+G+SY+S  V F VLDMFNLRAI +D 
Sbjct: 82  IVAAAHFTHIQATINCAKKLGLQLRIRSGGHDYDGMSYLS-TVDFVVLDMFNLRAIEIDP 141

Query: 139 KTETAWVQTGATLGEVYYRIAEKS-KFHGFPAGVCPTVGVGGHFGGGGYGNMMRKYGLSV 198
           K +TAWVQ+GATLGE+YY +A KS    GFPAG+CP +G GGHF GGGYGNMMRKYGLS+
Sbjct: 142 KLDTAWVQSGATLGEIYYNVANKSNNLRGFPAGICPGLGAGGHFSGGGYGNMMRKYGLSI 201

Query: 199 DQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVRVPETVTVFRVG 258
           D IIDAKIVD N ++LDR +MGEDLFWA+ GGG +SF VV A+KI+ V VPE VTVF V 
Sbjct: 202 DNIIDAKIVDANARVLDRSSMGEDLFWALRGGGAASFCVVLAWKIKLVPVPEKVTVFNV- 261

Query: 259 RTLEQNDV--TEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARFIALFLGDSE 318
            T+    V  T++  +WQ +A  IDNDLFIR+T    N      KT++A F+ ++LG+SE
Sbjct: 262 ETIGNRGVIPTDLAAKWQEIADKIDNDLFIRLTLSSSN------KTVKASFMGMYLGNSE 321

Query: 319 RLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTS-VDELLSRKPQVLTHLKRKS 378
           +LL ++   FP+LGL K++C+EM W+ESVLFW   P GT+    +L+R PQ   +LKRKS
Sbjct: 322 KLLEIMNAKFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRIPQKQIYLKRKS 381

Query: 379 DYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRAGNLWKIQYAT 438
           DYV+ PI K GL  I+K + E     + +NPYGGRM+EIP+  T FPHRAGN++KIQY++
Sbjct: 382 DYVQKPISKPGLESIFKILSENENVSMAWNPYGGRMSEIPATETAFPHRAGNMFKIQYSS 441

Query: 439 NWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNGKKSYYEGRVF 498
           NW   G E A+  +  T ++++ M+P+VSKNPR AFLNYRD+DIG N N   +Y EG+V+
Sbjct: 442 NWFVPGEEAASDCLSQTERVFEAMSPYVSKNPREAFLNYRDIDIGKNLN--STYEEGKVY 501

Query: 499 GLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           G+KYF+ NFERLV++KTRVDP+N FR EQSIP   HV R
Sbjct: 502 GVKYFKNNFERLVQVKTRVDPDNIFRYEQSIP--VHVSR 527

BLAST of Spg027087 vs. ExPASy TrEMBL
Match: A0A6J1K3U3 (berberine bridge enzyme-like 8 OS=Cucurbita maxima OX=3661 GN=LOC111490407 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.5e-281
Identity = 478/533 (89.68%), Postives = 504/533 (94.56%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M I R SI LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MEISRFSIFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKTETAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDV G LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVYGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG+++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKKSDCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKSDCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKEKLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP+NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPHNFFRNEQSIPRFPYVGR 535

BLAST of Spg027087 vs. ExPASy TrEMBL
Match: A0A6J1GSQ1 (berberine bridge enzyme-like 8 OS=Cucurbita moschata OX=3662 GN=LOC111456785 PE=3 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.8e-281
Identity = 477/533 (89.49%), Postives = 503/533 (94.37%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 60
           M I R S  LLS S+LLFFASAT APENFLQCLL  SPPS+PIAPAIFT NNASYSSVL+
Sbjct: 3   MDISRFSSFLLSFSALLFFASATTAPENFLQCLLRHSPPSYPIAPAIFTSNNASYSSVLE 62

Query: 61  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 120
           TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIH+LQMKIRSGGHDYEGVSYVSDV
Sbjct: 63  TYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHDLQMKIRSGGHDYEGVSYVSDV 122

Query: 121 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 180
           VPFFVLDMFNLR I+VDVKTETAWVQTGATLGEVYYRIAEK KFHGFPAGVCPTVGVGGH
Sbjct: 123 VPFFVLDMFNLRDISVDVKTETAWVQTGATLGEVYYRIAEKIKFHGFPAGVCPTVGVGGH 182

Query: 181 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 240
           FGGGGYGNMMRKYGLSVDQIIDAKIVDVNG LLDRKAMGEDLFWAI GGGGSSF VV AY
Sbjct: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGNLLDRKAMGEDLFWAIIGGGGSSFGVVVAY 242

Query: 241 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 300
           KIRTVRVPETVTVFRVGRTLE NDVTEIVD+WQRVAHVID DLFIRVTFDVVNG+++GKK
Sbjct: 243 KIRTVRVPETVTVFRVGRTLEDNDVTEIVDQWQRVAHVIDKDLFIRVTFDVVNGSRSGKK 302

Query: 301 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 360
           TLRA FIALFLGDSERLLSV+ KSFPKLGLKK+DCLEMSWL+SVLFWT+FP GTSVDELL
Sbjct: 303 TLRATFIALFLGDSERLLSVMNKSFPKLGLKKADCLEMSWLQSVLFWTNFPQGTSVDELL 362

Query: 361 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 420
           SR PQVLTHLKRKSDYV+  IPKE LNQIWKKMIEL KP +TFNPYGGRMAEIPSNATPF
Sbjct: 363 SRTPQVLTHLKRKSDYVKTLIPKENLNQIWKKMIELEKPTMTFNPYGGRMAEIPSNATPF 422

Query: 421 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 480
           PHRAGNLWKIQYATNW+EQGT+QANHFIDLTRKLY FMTPFV+KNPRTAFLNYRDLDIG+
Sbjct: 423 PHRAGNLWKIQYATNWDEQGTQQANHFIDLTRKLYKFMTPFVAKNPRTAFLNYRDLDIGV 482

Query: 481 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK+SYYEGRVFG+KYF+GNF+RLVKIKT+VDP NFFRNEQSIPRFP+VGR
Sbjct: 483 NHNGKRSYYEGRVFGIKYFEGNFDRLVKIKTKVDPLNFFRNEQSIPRFPYVGR 535

BLAST of Spg027087 vs. ExPASy TrEMBL
Match: A0A5A7TVZ2 (Reticuline oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold466G001070 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 466/532 (87.59%), Postives = 498/532 (93.61%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASA-TIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVL 60
           M ILRSSIL LS S L+FF+SA + APENFLQCLL  SPPS+PIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  QTYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           +TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR ITVD KTETAWVQTGATLGEVYYR+AEK+K +GFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAI GGGGSSF VV A
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGK 300
           YKIR VRVPE VTVFRV RTLEQN++TEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT   K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDEL 360
           KTLRA FIALFLGDS RLL V+  SFPKLGLKKSDC+EMSWL+SVLFWT+FPLGTSV+ L
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYV+APIPKEGL++IWKKMIEL KPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW+E+G  +A HFIDLTRKLY FMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHV 532
           +NHNGK SYYEGRV+G+KYF+GNFERLVKIKT+VDP+NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Spg027087 vs. ExPASy TrEMBL
Match: A0A1S3BE21 (reticuline oxidase-like protein OS=Cucumis melo OX=3656 GN=LOC103488597 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 466/532 (87.59%), Postives = 498/532 (93.61%), Query Frame = 0

Query: 1   MGILRSSILLLSISSLLFFASA-TIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVL 60
           M ILRSSIL LS S L+FF+SA + APENFLQCLL  SPPS+PIAPAIFTPNNASYSSVL
Sbjct: 1   MQILRSSILFLSFSFLVFFSSASSSAPENFLQCLLRHSPPSYPIAPAIFTPNNASYSSVL 60

Query: 61  QTYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSD 120
           +TYIRNLRFNTSTTPKPFLI+TALHESHVKV ILCARIHNLQMKIRSGGHDYEGVSYVSD
Sbjct: 61  ETYIRNLRFNTSTTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSD 120

Query: 121 VVPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGG 180
           VVPFFVLDMFNLR ITVD KTETAWVQTGATLGEVYYR+AEK+K +GFPAGVCPTVGVGG
Sbjct: 121 VVPFFVLDMFNLREITVDAKTETAWVQTGATLGEVYYRVAEKNKSYGFPAGVCPTVGVGG 180

Query: 181 HFGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTA 240
           HFGGGGYGNMMRK+GLSVDQIIDAKI+DVNGKLLDRKAMGEDLFWAI GGGGSSF VV A
Sbjct: 181 HFGGGGYGNMMRKHGLSVDQIIDAKIIDVNGKLLDRKAMGEDLFWAIIGGGGSSFGVVVA 240

Query: 241 YKIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGK 300
           YKIR VRVPE VTVFRV RTLEQN++TEIVDEWQ+VAHVIDNDLFIRVTFDVVNGT   K
Sbjct: 241 YKIRMVRVPERVTVFRVQRTLEQNELTEIVDEWQQVAHVIDNDLFIRVTFDVVNGTDKEK 300

Query: 301 KTLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDEL 360
           KTLRA FIALFLGDS RLL V+  SFPKLGLKKSDC+EMSWL+SVLFWT+FPLGTSV+ L
Sbjct: 301 KTLRATFIALFLGDSGRLLFVINNSFPKLGLKKSDCIEMSWLQSVLFWTNFPLGTSVEAL 360

Query: 361 LSRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATP 420
           LSR PQVLTHLKRKSDYV+APIPKEGL++IWKKMIEL KPM+TFNPYGGRMAEIPSNATP
Sbjct: 361 LSRTPQVLTHLKRKSDYVKAPIPKEGLDKIWKKMIELEKPMMTFNPYGGRMAEIPSNATP 420

Query: 421 FPHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIG 480
           FPHRAGNLWKIQYATNW+E+G  +A HFIDLTRKLY FMTPFVSKNPRTAFLNYRDLDIG
Sbjct: 421 FPHRAGNLWKIQYATNWDEEGKREAKHFIDLTRKLYKFMTPFVSKNPRTAFLNYRDLDIG 480

Query: 481 INHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHV 532
           +NHNGK SYYEGRV+G+KYF+GNFERLVKIKT+VDP+NFFRNEQSIPRFPHV
Sbjct: 481 VNHNGKNSYYEGRVYGIKYFEGNFERLVKIKTKVDPHNFFRNEQSIPRFPHV 532

BLAST of Spg027087 vs. ExPASy TrEMBL
Match: A0A6J1DGF3 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020214 PE=3 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.3e-272
Identity = 466/533 (87.43%), Postives = 501/533 (94.00%), Query Frame = 0

Query: 3   ILRSSILLLSISSLLFFASA--TIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQ 62
           ILR   LL  + SLLFFASA  T +PENFL+CLL RSPPSHPI+PAIFTP+NAS+SSVLQ
Sbjct: 5   ILRPFFLLSLLFSLLFFASATTTASPENFLKCLLRRSPPSHPISPAIFTPDNASFSSVLQ 64

Query: 63  TYIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDV 122
           TYIRNLRFNTS+TPKPFLI+TALHESHVKV ILCARIHNLQMKIRSGGHDYEGVSYVSDV
Sbjct: 65  TYIRNLRFNTSSTPKPFLILTALHESHVKVAILCARIHNLQMKIRSGGHDYEGVSYVSDV 124

Query: 123 VPFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGH 182
           VPFFVLDMFNLRAITVDV+TETAWV+TGATLGEVYYRIAEKSK HGFPAGVCPTVGVGGH
Sbjct: 125 VPFFVLDMFNLRAITVDVETETAWVETGATLGEVYYRIAEKSKLHGFPAGVCPTVGVGGH 184

Query: 183 FGGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAY 242
           FGGGGYGNMMRKYGLSVDQIIDAK+VDVNGKLLDRK+MGEDLFWAITGGGGSSF  V AY
Sbjct: 185 FGGGGYGNMMRKYGLSVDQIIDAKMVDVNGKLLDRKSMGEDLFWAITGGGGSSFGXVVAY 244

Query: 243 KIRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKK 302
           KI+TVRVPETVTVF+V R L +N VTEIVDEWQRVAH IDNDLFIRVTFDVVNGT+NGKK
Sbjct: 245 KIQTVRVPETVTVFKVERRLAEN-VTEIVDEWQRVAHEIDNDLFIRVTFDVVNGTENGKK 304

Query: 303 TLRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELL 362
           TLRA FIALFLGDSERLLS+++KSFPKLGLK+SDCL+MSWLESVLFWT+FPLGTSVD LL
Sbjct: 305 TLRATFIALFLGDSERLLSIMDKSFPKLGLKQSDCLQMSWLESVLFWTNFPLGTSVDALL 364

Query: 363 SRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPF 422
           SRKPQVLTHLKRKSDYV+  IPKEGLNQIWKKMIEL KPMLTFNPYGGRMAEI S+ TPF
Sbjct: 365 SRKPQVLTHLKRKSDYVKTLIPKEGLNQIWKKMIELEKPMLTFNPYGGRMAEIASSKTPF 424

Query: 423 PHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGI 482
           PHRAGNLWKIQYATNW+E+G E+A+HFI+LTRKLY+FMTPFVSKNPRTAFLNYRDLDIG+
Sbjct: 425 PHRAGNLWKIQYATNWDEEGVEEADHFINLTRKLYNFMTPFVSKNPRTAFLNYRDLDIGV 484

Query: 483 NHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFPHVGR 534
           NHNGK SY EGRVFG+KYF+GNF+RLVKIKTRVDPNNFFRNEQSIPR P+V R
Sbjct: 485 NHNGKNSYNEGRVFGVKYFEGNFDRLVKIKTRVDPNNFFRNEQSIPRLPYVKR 536

BLAST of Spg027087 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 676.0 bits (1743), Expect = 2.4e-194
Identity = 332/513 (64.72%), Postives = 397/513 (77.39%), Query Frame = 0

Query: 20  ASATIAPENFLQCLLHRSPPSHPIAPAIFTPNNASYSSVLQTYIRNLRFNTSTTPKPFLI 79
           +S++   E F QCL   S P HPI+PAIF   N SYSSVLQ  IRNLRFNT++TPKPFLI
Sbjct: 19  SSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLI 78

Query: 80  VTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVS-DVVPFFVLDMFNLRAITVDV 139
           + A HESHV+  I C + HNLQMKIRSGGHDY+G+SYV+    PFFVLDMFNLR++ VDV
Sbjct: 79  IAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDV 138

Query: 140 KTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGYGNMMRKYGLSVD 199
            ++TAWVQTGA LGEVYY I EKSK   +PAG+CPTVGVGGH  GGGYGNMMRKYGL+VD
Sbjct: 139 ASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVD 198

Query: 200 QIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVRVPETVTVFRVGR 259
             IDA++VDVNGK+LDRK MGEDL+WAI GGGG S+ VV AYKI  V VPE VTVFR+ R
Sbjct: 199 NTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISR 258

Query: 260 TLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARFIALFLGDSERLL 319
           TLEQN  T+I+  WQ+VA  + ++LFIR   DVVNGT + +KT+R  FIA+FLGD+  LL
Sbjct: 259 TLEQN-ATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLL 318

Query: 320 SVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDELLSRKPQVLTHLKRKSDYVR 379
           S++ + FP+LGL +SDC E SW++SVLFWT+  +G+S + LL ++ Q + +LKRKSDYVR
Sbjct: 319 SILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSS-ETLLLQRNQPVNYLKRKSDYVR 378

Query: 380 APIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRAGNLWKIQYATNWNE 439
            PI + GL  IWKKMIEL  P + FNPYGG M  I S  TPFP+RAGNLWKIQY  NW +
Sbjct: 379 EPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD 438

Query: 440 QGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGIN-HNGK-KSYYEGRVFGL 499
           +     + +++LTRKLY FMTPFVSKNPR +F NYRD+D+GIN HNGK  SY EG+ +G 
Sbjct: 439 E--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGK 498

Query: 500 KYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFP 530
           KYF GNFERLVKIKTRVD  NFFRNEQSIP  P
Sbjct: 499 KYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Spg027087 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 575.5 bits (1482), Expect = 4.5e-164
Identity = 293/522 (56.13%), Postives = 374/522 (71.65%), Query Frame = 0

Query: 15  SLLFFASATIAP-------ENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQTYIRNL 74
           SL FF+SA  AP       E+F+QC   ++  P   I   +F+  N S+SSVL+ YIRN 
Sbjct: 19  SLPFFSSA--APPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNG 78

Query: 75  RFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 134
           RFNTS+TPKP +IVT   + HV   + C++  N  +KIRSGGHDYEG+SY+SD  PFF+L
Sbjct: 79  RFNTSSTPKPAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISD-KPFFIL 138

Query: 135 DMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGY 194
           DM NLR ++VD+  ++AW+  GATLGEVYYRI EKSK HGFPAGVCPTVGVGGH  GGGY
Sbjct: 139 DMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHISGGGY 198

Query: 195 GNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVR 254
           GNM+RK+GLSVD +IDAKIVDVNG++LDRK+MGEDLFWAI+GGGG+SF VV  YK++ V 
Sbjct: 199 GNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVP 258

Query: 255 VPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARF 314
           VPETVTVFRV + ++   V ++V +WQ V    D +LF+R+    V  T+   KT+RA  
Sbjct: 259 VPETVTVFRVEKYMDSGAV-DMVHKWQSVGPKTDRNLFLRMLIQPV--TRKKVKTVRATV 318

Query: 315 IALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDE--LLSRKP 374
           +ALFLG +E +++++ K FP+L LKK +C EM+W +S L+W +    T +D    L R  
Sbjct: 319 VALFLGRAEEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNL 378

Query: 375 QVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRA 434
                 KRKSDYV + IP++G+  ++KKM ELGK  L FNPYGG+MAE+  NATPFPHR+
Sbjct: 379 DRANFGKRKSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRS 438

Query: 435 GNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNG 494
             L+KIQY+  W E   E    F++    LY FMT FVSKNPR A+LNYRD+DIG+N +G
Sbjct: 439 -KLFKIQYSVTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHG 498

Query: 495 KKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIP 527
             SY EG V+G KYF  NF+RLVK+KT  DP+NFFRNEQSIP
Sbjct: 499 TNSYEEGEVYGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIP 533

BLAST of Spg027087 vs. TAIR 10
Match: AT1G11770.1 (FAD-binding Berberine family protein )

HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-163
Identity = 290/530 (54.72%), Postives = 375/530 (70.75%), Query Frame = 0

Query: 8   ILLLSISSLLFFASATIAP-----ENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQT 67
           ++L  ISS +  + A   P     +NFLQC  +++  P + +A  +     A+++ VL+ 
Sbjct: 6   LILFLISSFISTSLAVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRA 65

Query: 68  YIRNLRFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVV 127
           YIRN RFNT+ TPKP +++ A  ESHV+  ++C +  N+Q+K RSGGHDYEGVSY+S  V
Sbjct: 66  YIRNARFNTTATPKPAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISH-V 125

Query: 128 PFFVLDMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHF 187
           PFFVLDM NLR ITVD  TE+AWV  GATLGEVYYRI EK+K HGFPAGVCPTVG GGH 
Sbjct: 126 PFFVLDMSNLRNITVDPATESAWVGAGATLGEVYYRIWEKTKSHGFPAGVCPTVGAGGHI 185

Query: 188 GGGGYGNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYK 247
            GGGYGNM+RKYGLSVD + DAKIVDVNG++LDRK MGED+FWAI GGGG+SF V+ A+K
Sbjct: 186 SGGGYGNMIRKYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFK 245

Query: 248 IRTVRVPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKT 307
           I+ V VP TVTVFRV + L +N  TE+V +WQ VA   D  LF+R+    V  T+N  +T
Sbjct: 246 IKLVPVPPTVTVFRVEKNLVEN-ATEMVHKWQFVAPKTDPGLFMRLLLQPV--TRNKMQT 305

Query: 308 LRARFIALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSV--DEL 367
           +RA  +ALFLGD   ++S++ K FP+LGLKK +C EM+W++SV++W +    T +  + L
Sbjct: 306 VRASVVALFLGDQNTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEIL 365

Query: 368 LSRKPQVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATP 427
           L R P + T  KRKSD+V   I K+GL+ ++KKMIE+GK  L FNPYGG M+ + +  TP
Sbjct: 366 LDRNPDMATFGKRKSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTP 425

Query: 428 FPHRAGNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIG 487
           FPHR   L+KIQ++ NW + GTE    F+   +  Y +M PFV+KNPR  ++NYRDLDIG
Sbjct: 426 FPHRK-KLYKIQHSMNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIG 485

Query: 488 INHNGKKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIPRFP 530
           +N  G  SY    VFG  YF  NF+RLVK+KT VDP NFFR+EQSIP  P
Sbjct: 486 VNTPGPNSYRVAEVFGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLP 530

BLAST of Spg027087 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 7.9e-161
Identity = 285/522 (54.60%), Postives = 371/522 (71.07%), Query Frame = 0

Query: 8   ILLLSISSLLFFASATIAPENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQTYIRNL 67
           +L LS+  L  F+ +     +FL+C   ++  P   I   +F+  N ++SSVL+ YIRN 
Sbjct: 19  LLFLSL-PLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 78

Query: 68  RFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 127
           RFNTS+T KP +I+T   ESHV   + C++  N  +KIRSGGHDY+G+SY+SD  PFF+L
Sbjct: 79  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFIL 138

Query: 128 DMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGY 187
           DM N+R ++VD+ + +AW+  GATLGEVYYRI EKS+ HGFPAGVCPTVGVGGH  GGGY
Sbjct: 139 DMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGY 198

Query: 188 GNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVR 247
           GNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAITGGGG S+ VV  YK++ V 
Sbjct: 199 GNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVP 258

Query: 248 VPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARF 307
           VP  VTVFRV + ++   V ++V +WQ V    D +LF+R+    V  T+   KT+RA  
Sbjct: 259 VPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASV 318

Query: 308 IALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDE--LLSRKP 367
           +ALFLG ++ +++++ K FP+LGLKK +C EM+W +S L+W +    T VD    L R  
Sbjct: 319 VALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNL 378

Query: 368 QVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRA 427
              +  KRKSDYV   IPK+G+  ++KKMIELGK  L FNPYGG+MAE+  NA PFPHR 
Sbjct: 379 DTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR- 438

Query: 428 GNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNG 487
             L+KIQY+ NW E   E    +++  + LY FMT FVSKNPR+++ NYRD+DIG+N +G
Sbjct: 439 NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHG 498

Query: 488 KKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIP 527
             SY EG V+G KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 ANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534

BLAST of Spg027087 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 564.7 bits (1454), Expect = 7.9e-161
Identity = 285/522 (54.60%), Postives = 371/522 (71.07%), Query Frame = 0

Query: 8   ILLLSISSLLFFASATIAPENFLQCLLHRS-PPSHPIAPAIFTPNNASYSSVLQTYIRNL 67
           +L LS+  L  F+ +     +FL+C   ++  P   I   +F+  N ++SSVL+ YIRN 
Sbjct: 19  LLFLSL-PLSSFSQSNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNA 78

Query: 68  RFNTSTTPKPFLIVTALHESHVKVVILCARIHNLQMKIRSGGHDYEGVSYVSDVVPFFVL 127
           RFNTS+T KP +I+T   ESHV   + C++  N  +KIRSGGHDY+G+SY+SD  PFF+L
Sbjct: 79  RFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISD-KPFFIL 138

Query: 128 DMFNLRAITVDVKTETAWVQTGATLGEVYYRIAEKSKFHGFPAGVCPTVGVGGHFGGGGY 187
           DM N+R ++VD+ + +AW+  GATLGEVYYRI EKS+ HGFPAGVCPTVGVGGH  GGGY
Sbjct: 139 DMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGGGY 198

Query: 188 GNMMRKYGLSVDQIIDAKIVDVNGKLLDRKAMGEDLFWAITGGGGSSFSVVTAYKIRTVR 247
           GNM+RK+GLSVD + DAKIVDVNG++LDRKAMGEDLFWAITGGGG S+ VV  YK++ V 
Sbjct: 199 GNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVP 258

Query: 248 VPETVTVFRVGRTLEQNDVTEIVDEWQRVAHVIDNDLFIRVTFDVVNGTQNGKKTLRARF 307
           VP  VTVFRV + ++   V ++V +WQ V    D +LF+R+    V  T+   KT+RA  
Sbjct: 259 VPSVVTVFRVEQYMDSGAV-DMVHKWQSVGPKTDPNLFMRMLIQPV--TRKKVKTVRASV 318

Query: 308 IALFLGDSERLLSVVEKSFPKLGLKKSDCLEMSWLESVLFWTDFPLGTSVDE--LLSRKP 367
           +ALFLG ++ +++++ K FP+LGLKK +C EM+W +S L+W +    T VD    L R  
Sbjct: 319 VALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNL 378

Query: 368 QVLTHLKRKSDYVRAPIPKEGLNQIWKKMIELGKPMLTFNPYGGRMAEIPSNATPFPHRA 427
              +  KRKSDYV   IPK+G+  ++KKMIELGK  L FNPYGG+MAE+  NA PFPHR 
Sbjct: 379 DTSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHR- 438

Query: 428 GNLWKIQYATNWNEQGTEQANHFIDLTRKLYDFMTPFVSKNPRTAFLNYRDLDIGINHNG 487
             L+KIQY+ NW E   E    +++  + LY FMT FVSKNPR+++ NYRD+DIG+N +G
Sbjct: 439 NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHG 498

Query: 488 KKSYYEGRVFGLKYFQGNFERLVKIKTRVDPNNFFRNEQSIP 527
             SY EG V+G KYF  NF+RLVKIKT VDP NFFRNEQSIP
Sbjct: 499 ANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIP 534