Spg026319 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg026319
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold13: 17162266 .. 17164571 (+)
RNA-Seq ExpressionSpg026319
SyntenySpg026319
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAATGAAAATGGAAAATGTAGCATTCGGTGCCTAGTATTAAAGGAAGGAACCCACCACCGCTCAAGGTCTTCTCAGCCCATTTTCTCCATCTTTTTCCATCAAAACTTCAGAGCATTCGAGGGAGAGAGTGAAGGAAGAATAAGAAGAAGCTACCTAGGGTTTTGAGGTTGAAGAAGAGAGGAAATAACTCAAGCAAAGGCAACCATGGCAGAATCTAGGTTCAACCTGCCTATCTCTGGTTTTTTATTCGATTTGAGCTACACAGAAAGAACTAAGAGGTGAGGTCTGATTTTCTTGAAAGTTAATCCATTTTCTAACAAATTTCATGACGGAAGCTTGAGCTAAATGTGATGATAAGTTAATGGTTTTTGAATTGGAAAACAGAGCACATAATTTTCGTGCAAACCAGGAGTATCTTTGGTTTTCTCTGGTTTTTCGAGCATTCTGGGGTGTACAAGTTGAATCAGAAGCTCCATTTGGTATGGTTGGAAAGCTTATTCCATTTCCTATAACTTACATGAAGAATTCATGAGCCACAAATGACTTGAAGTAGTTTTAATTTCAGGAGAAAGATAGAGGGTAGCAGAGAAGCTCGAATCTATACTTGTTGTGTTCGGGGGATTGTTGGACAAATCTGTTGGGAATCTCGTTTTCATTCCTTCAGGTAAGTTTTAGAGGGTTCCAAAATAAAGAATTGGATATGTAGTACATTAAATTTGGTCAAGTATTGAGGTAGTTATGAGTGTTGGGAGTCAGGGTATTGAATCTGGAAAATCTGGGAAAAAGAATGTGAAGTGTGATTTTAATTCAAGTGTTTAGAATTCATGAGCATGGAAAGACAAGACTACCATTGGTTTTGATGCTCGGTTTTGATTTTTTTGACAGGCAAAGAGGAAATAGGCCGAGTTTACAGACCAGGGAACTAACCAAGACTGTGAGTGAAAAAACGAATTTCGAAAATGGTTTCTAAACTGTTATTTATGCTTAAATGGTTTACATACTTGAATTTGAACTTATGAATGATACATGCTTTTCTATGAATGGTTTCAAGCATAAGTTTTGAGCTTTGATTTTCTAATGAGATTTATATACGAGCACGTGATTAATGACTTTCTGAAAAGTAGTTGAAACGATGTCATTTTAAGGAAAGTATGTTTTAGCGAGTATTGTTTAAGATTTTCAGTTTTCCACGAGCAAGCTTAGTAAGCTGGAGTTTTACGAAAGATAAAGATAAGCATACATGTTTTAATGTTTTCATGTGGTTTTCTGAGATTTTTATTGACTACATGACTGAGATATTGAGTCTGAGGCTATAAGGTACCGTGTGCACACAGGTTATATTACGTTGTTAACGTTGAGTGCTCCGTGACAACGATGTTGTCGTGAGTGCTGGGCGGGCCCCACTACTTCGTAGAGCTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAAAGTTGATTTGTCATGTTAGTTAAAATGTTTTATATACTTGATATGCCCTGCTAGATTTTAATGACGTACTTGCAGAATATTTGAGAAAGCTTTTATCCTTACATGTTTATGTTTAACGCTTTGATTGAATAGATTTATACGTTTAAAGGTTTATCATGTGTTCTGATTTGCGGATTTACAGTTTTAAATTTAGTCACTCACTGGGCTTCGTAGTTCACTCTTTAAAATTTTTTTACCACTTTTTAGGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTACCAAGTCGACTTTCAGTATGTGGGTGGAGTTGTACATTGAGTCTTTTTGTATTGTAAATGTCTTTGTAAGAACTGGATAGTTTATGGTTGTGTAAGCACTAGATGTCGTGGTTGTGCAAACCCCAGTTGGATACGTTTTTTGTTGTGATATCTCTACCAGGTTATTTTTCAAACCGAGTTATGTCAGGGCTACATCCCATGTATGTCTAAAGTTTTCAGGGATTCCGCTGTGTTGCGTTACATGTAAAGTATTTTATTTCTAAGACTTGTATGTTTATCAGGTTCATCGATTCAGCAGGGTCAACAGGTATCGTTAGAGGAGGACGATATCTGTTGGCTTCACGCCATCTCCCTGACTAAGCTAGCAGGTAGTTTGGGATGGGGTGTGACAACTTGGTATTAGAGCAGTTAGCTCCATGGGAAATGAGACATGGCAGTTAGTGCTAAGAAGAATCTAGGAAGTGAACAGTTAGACGATGAGTCAGTTCAGATAGAACTAGCGGAGTACGGTCCAAGTATTGTTGAGAATCAGTCCAGTCAGGACTAG

mRNA sequence

ATGGCAGAATCTAGGTTCAACCTGCCTATCTCTGGTTTTTTATTCGATTTGAGCTACACAGAAAGAACTAAGAGGAGAAAGATAGAGGGTAGCAGAGAAGCTCGAATCTATACTTGTTGTGTTCGGGGGATTGTTGGACAAATCTGTTGGGAATCTCGTTTTCATTCCTTCAGGCAAAGAGGAAATAGGCCGAGTTTACAGACCAGGGAACTAACCAAGACTGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTACCAAGTCGACTTTCAGTTCATCGATTCAGCAGGGTCAACAGGTATCGTTAGAGGAGGACGATATCTGTTGGCTTCACGCCATCTCCCTGACTAAGCTAGCAGTTAGTGCTAAGAAGAATCTAGGAAGTGAACAGTTAGACGATGAGTCAGTTCAGATAGAACTAGCGGAGTACGGTCCAAGTATTGTTGAGAATCAGTCCAGTCAGGACTAG

Coding sequence (CDS)

ATGGCAGAATCTAGGTTCAACCTGCCTATCTCTGGTTTTTTATTCGATTTGAGCTACACAGAAAGAACTAAGAGGAGAAAGATAGAGGGTAGCAGAGAAGCTCGAATCTATACTTGTTGTGTTCGGGGGATTGTTGGACAAATCTGTTGGGAATCTCGTTTTCATTCCTTCAGGCAAAGAGGAAATAGGCCGAGTTTACAGACCAGGGAACTAACCAAGACTGTAGAGATCGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTACCAAGTCGACTTTCAGTTCATCGATTCAGCAGGGTCAACAGGTATCGTTAGAGGAGGACGATATCTGTTGGCTTCACGCCATCTCCCTGACTAAGCTAGCAGTTAGTGCTAAGAAGAATCTAGGAAGTGAACAGTTAGACGATGAGTCAGTTCAGATAGAACTAGCGGAGTACGGTCCAAGTATTGTTGAGAATCAGTCCAGTCAGGACTAG

Protein sequence

MAESRFNLPISGFLFDLSYTERTKRRKIEGSREARIYTCCVRGIVGQICWESRFHSFRQRGNRPSLQTRELTKTVEIELPVPDTLPTSAESTKSTFSSSIQQGQQVSLEEDDICWLHAISLTKLAVSAKKNLGSEQLDDESVQIELAEYGPSIVENQSSQD
Homology
[Close]
BLAST of Spg026319 vs. NCBI nr
Match: KAA0052218.1 (uncharacterized protein E6C27_scaffold207G00290 [Cucumis melo var. makuwa] >TYK01785.1 uncharacterized protein E5676_scaffold113G00130 [Cucumis melo var. makuwa])

HSP 1 Score: 68.2 bits (165), Expect = 7.6e-08
Identity = 42/98 (42.86%), Postives = 52/98 (53.06%), Query Frame = 0 

Query: 56  SFRQRGNRPSLQTRELTKTVEIELPVPDTLPTSAESTKSTFSSSIQ-------------- 115
           S   RGNRP+L T +L KTVEIELPVPDTLPTSAES+KS  S+ ++              
Sbjct: 76  SILPRGNRPNLHTGKLDKTVEIELPVPDTLPTSAESSKSNSSTWLELYFESVHVEMLFYK 135

Query: 116 ------------QGQQVSLEEDDICWLHAISLTKLAVS 128
                       +G    +  DD+CWLHA+   K A S
Sbjct: 136 PSDVRVASYVCWEGSAGIVRGDDLCWLHAVFWAKTAGS 173

[Close]
BLAST of Spg026319 vs. NCBI nr
Match: KAA0060199.1 (uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ95571.1 uncharacterized protein E5676_scaffold767G00770 [Cucumis melo var. makuwa])

HSP 1 Score: 65.5 bits (158), Expect = 4.9e-07
Identity = 43/91 (47.25%), Postives = 52/91 (57.14%), Query Frame = 0 

Query: 53  RFHSFRQRGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKS- 112
           + +  R RGNRPSLQT +L KT                 VEIELPVPDTLPTSAES +S 
Sbjct: 49  KLYLVRPRGNRPSLQTGKLDKTVIFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESN 108

Query: 113 TFSSSIQQGQQVSLEEDDICWLHAISLTKLA 126
           + S+ I +G       DD+CWLHA+   K A
Sbjct: 109 SRSTGIVRG-------DDVCWLHAVFRAKTA 132

[Close]
BLAST of Spg026319 vs. NCBI nr
Match: KAA0066795.1 (uncharacterized protein E6C27_scaffold271G001440 [Cucumis melo var. makuwa] >TYK27942.1 uncharacterized protein E5676_scaffold384G001370 [Cucumis melo var. makuwa])

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-06
Identity = 51/131 (38.93%), Postives = 66/131 (50.38%), Query Frame = 0 

Query: 14  LFDLSYTERTKRRKIEGSREARIYTCCVRGIVGQICWESRFHSFRQRGNRPSLQTRELTK 73
           LF LSY E TK           +       ++     + + +    RGNRPSLQTREL K
Sbjct: 19  LFGLSYLEETKSTGSSSKPAGVLVFSDALSMLEVHKSDQKLYLVWPRGNRPSLQTRELDK 78

Query: 74  TVEIELPVPDTLPTSAESTKSTFSSSIQ------------QGQQVSLEEDDICWLHAISL 133
           T  IELPVPDTLPTSAES+ S  S+ ++            +GQQ SLEE  +    + + 
Sbjct: 79  T--IELPVPDTLPTSAESSGSNSSTWLELYFESVHVEMFYKGQQGSLEETHL----SQAA 138

[Close]
BLAST of Spg026319 vs. NCBI nr
Match: KAA0042035.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 62.8 bits (151), Expect = 3.2e-06
Identity = 40/91 (43.96%), Postives = 49/91 (53.85%), Query Frame = 0 

Query: 60  RGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKSTFSSSIQQ 119
           RGNRPSLQT EL KT                 VEIELP+PDTLPTSAES++S  S+ ++ 
Sbjct: 124 RGNRPSLQTGELDKTVIFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLEL 183

Query: 120 ----------GQQVSLEEDDICWLHAISLTK 124
                     G    +  DD+CWLHA+   K
Sbjct: 184 YFESVHVEMFGSVGIIRGDDVCWLHAVFRAK 214

[Close]
BLAST of Spg026319 vs. NCBI nr
Match: TYK17971.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 62.8 bits (151), Expect = 3.2e-06
Identity = 40/91 (43.96%), Postives = 49/91 (53.85%), Query Frame = 0 

Query: 60  RGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKSTFSSSIQQ 119
           RGNRPSLQT EL KT                 VEIELP+PDTLPTSAES++S  S+ ++ 
Sbjct: 124 RGNRPSLQTGELDKTVIFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLEL 183

Query: 120 ----------GQQVSLEEDDICWLHAISLTK 124
                     G    +  DD+CWLHA+   K
Sbjct: 184 YFESVHVEMFGSVGIIRGDDVCWLHAVFRAK 214

[Close]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match: A0A5A7UAH6 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00130 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.7e-08
Identity = 42/98 (42.86%), Postives = 52/98 (53.06%), Query Frame = 0 

Query: 56  SFRQRGNRPSLQTRELTKTVEIELPVPDTLPTSAESTKSTFSSSIQ-------------- 115
           S   RGNRP+L T +L KTVEIELPVPDTLPTSAES+KS  S+ ++              
Sbjct: 76  SILPRGNRPNLHTGKLDKTVEIELPVPDTLPTSAESSKSNSSTWLELYFESVHVEMLFYK 135

Query: 116 ------------QGQQVSLEEDDICWLHAISLTKLAVS 128
                       +G    +  DD+CWLHA+   K A S
Sbjct: 136 PSDVRVASYVCWEGSAGIVRGDDLCWLHAVFWAKTAGS 173

[Close]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match: A0A5D3BAD1 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00770 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.4e-07
Identity = 43/91 (47.25%), Postives = 52/91 (57.14%), Query Frame = 0 

Query: 53  RFHSFRQRGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKS- 112
           + +  R RGNRPSLQT +L KT                 VEIELPVPDTLPTSAES +S 
Sbjct: 49  KLYLVRPRGNRPSLQTGKLDKTVIFSLSTLSVLRDNDAVVEIELPVPDTLPTSAESFESN 108

Query: 113 TFSSSIQQGQQVSLEEDDICWLHAISLTKLA 126
           + S+ I +G       DD+CWLHA+   K A
Sbjct: 109 SRSTGIVRG-------DDVCWLHAVFRAKTA 132

[Close]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match: A0A5D3DWP3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001370 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.0e-07
Identity = 51/131 (38.93%), Postives = 66/131 (50.38%), Query Frame = 0 

Query: 14  LFDLSYTERTKRRKIEGSREARIYTCCVRGIVGQICWESRFHSFRQRGNRPSLQTRELTK 73
           LF LSY E TK           +       ++     + + +    RGNRPSLQTREL K
Sbjct: 19  LFGLSYLEETKSTGSSSKPAGVLVFSDALSMLEVHKSDQKLYLVWPRGNRPSLQTRELDK 78

Query: 74  TVEIELPVPDTLPTSAESTKSTFSSSIQ------------QGQQVSLEEDDICWLHAISL 133
           T  IELPVPDTLPTSAES+ S  S+ ++            +GQQ SLEE  +    + + 
Sbjct: 79  T--IELPVPDTLPTSAESSGSNSSTWLELYFESVHVEMFYKGQQGSLEETHL----SQAA 138

[Close]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match: A0A5A7TKT0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G004880 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.5e-06
Identity = 40/91 (43.96%), Postives = 49/91 (53.85%), Query Frame = 0 

Query: 60  RGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKSTFSSSIQQ 119
           RGNRPSLQT EL KT                 VEIELP+PDTLPTSAES++S  S+ ++ 
Sbjct: 124 RGNRPSLQTGELDKTVIFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLEL 183

Query: 120 ----------GQQVSLEEDDICWLHAISLTK 124
                     G    +  DD+CWLHA+   K
Sbjct: 184 YFESVHVEMFGSVGIIRGDDVCWLHAVFRAK 214

[Close]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match: A0A5D3D150 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G002770 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.5e-06
Identity = 40/91 (43.96%), Postives = 49/91 (53.85%), Query Frame = 0 

Query: 60  RGNRPSLQTRELTKT-----------------VEIELPVPDTLPTSAESTKSTFSSSIQQ 119
           RGNRPSLQT EL KT                 VEIELP+PDTLPTSAES++S  S+ ++ 
Sbjct: 124 RGNRPSLQTGELDKTVIFSLSTLSVLRDNDTVVEIELPMPDTLPTSAESSESNSSTLLEL 183

Query: 120 ----------GQQVSLEEDDICWLHAISLTK 124
                     G    +  DD+CWLHA+   K
Sbjct: 184 YFESVHVEMFGSVGIIRGDDVCWLHAVFRAK 214

The following BLAST results are available for this feature:
BLAST of Spg026319 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0052218.17.6e-0842.86uncharacterized protein E6C27_scaffold207G00290 [Cucumis melo var. makuwa] >TYK0... [more]
KAA0060199.14.9e-0747.25uncharacterized protein E6C27_scaffold386G00120 [Cucumis melo var. makuwa] >TYJ9... [more]
KAA0066795.11.9e-0638.93uncharacterized protein E6C27_scaffold271G001440 [Cucumis melo var. makuwa] >TYK... [more]
KAA0042035.13.2e-0643.96DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
TYK17971.13.2e-0643.96DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
BLAST of Spg026319 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg026319 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7UAH63.7e-0842.86CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A5D3BAD12.4e-0747.25Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5D3DWP39.0e-0738.93Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7TKT01.5e-0643.96Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67... [more]
A0A5D3D1501.5e-0643.96Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold30... [more]
BLAST of Spg026319 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg026319.1Spg026319.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity