Spg026056 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg026056
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
Locationscaffold7: 2168898 .. 2172496 (+)
RNA-Seq ExpressionSpg026056
SyntenySpg026056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAGGTGTGTGCGTCGTACTGGAGTATTCCCCCCTCCAATTACAAATATTTATTACCATGCAAAGGCAAATCACAATCTTGTTTACCTTGTTGTGTTCTTTCTTTACTCCTCTTCTGGTCTTGTCCCCATTCGTACGTTATTGGTCCTCAACCCCAATGAACATGTCCAATCAGCTCGCGCCACGCTGCTGACTCTTCTGTTTTGTGTTGGGACACGTGGCTCCAAGTCTCGTCCACATCATACTCGAAAAATACAAAATTTATATAATTTATATACGTCGATCCCCCCTTGACTTGACAAATGACAAACAGACAATTTTCTTTTTCTGTACAGTTGTCCATTTATGGTCTTTGTCCAACATTTGTTGCTCCCATCCAATGCCCTTATAAACCTCCCTAGTTGGTGAACTTTTTATTCTCATTGACCAATTATAAGTATGTTGTAAGGGACAATGGACACGACACAGATCTCCACAATGGTATGATATTGTCCACTTTGGACATAAGCCCCCATGACTTTGCTTTTAGTTCACCCCAAAACGCTTCATACCAATGGAGATACATGTCTTCCCTTATAAGCCCATGATCCTTTCATTATCTAGCCAATGTGGGACTTTGGTCGCACTCCCAACAAATATATCTTCATTTTGAAAACATTATAAATCTTCTCTAGGTTCAATAAATATATAGATAGCATCATTGTTTGTTGAGCTTTCCCTTTTAGTTTGTGCAAAATTATAGATTATGTTAGAATAATGTGTTGAAGTTTTTTATGTGATGTGTAGGATATGATATTGGGGTTTTTTTAATAGGGATATCTTCAAGACCTTGGTTATTTAAAAGATAAGAATGACGTTGGTGATTAATTTATTATGGTTTTCAATGATTAAATAATTTGGTTTAGTTGACTCAACCATTATGATAAGGAATCTTTTGAAGACTTAATATATGAATATTTTTAAAAACTAAATGTAGAGTACTCCATCTTATTTTATTGTGGAGTAACTTAGATATGAAATTAATACAATTTTTTATGGATCTCGAGATATAAATTGAAACATGGTTTAAATGATTGTTTTTTTTTTTTTTTTTGTCGGAACGATATAGTTAAGGATATTGTACAAAGAGATTGAAGTTTTTGTTACCGTATTTGTATCAACTAGTCTTTATCGATTGACAAGAGAGTTATCGGGAAGGATTAGAGTATGAAAAAGAAATTTGATGAACATGAGACATGGCTTTAAATAGAGGTATTTGAGGGAAGGGAAATGGAGTTTGAACATCTCCAAAACGCGACATTAGATGGTCAAAACGACAGAAAAGTTGTCCGTATAAATAGGTAAAGTGGGGAGCCAATAGGGAAGAGCGAAAGAGTAAGACGTGGCGTGGAGAGTGTTCACATTAGTGTCAGTGGATTTCTGATAGAGAGAATATTGATTGTTTAGTACTGAGTCACTGACACCTGGCAGATTGCATTTCCTCCAACTCCAACTACCCTATCAATTTCATATATATATATTCGGGTACCCCTTCTAATGGGTCGGGTTTCTTCTAGGGCCCCACCCAATCATCCACAACTCACCCCATCATTATTTTATTATAATTTTTCTACCTGCCTTTTCTAAAAAAAAAAAAAACTTTTGTTGCAAAACTTGGCATTTAGTGAGGTTGTGTTTCTTTTATTAACTCCATTTTAATGCTTGCAAGAGATATTAATCATTTAAGGACTACATGATTGATAAGTTGATTTCAGATAACTTATTTTATTAAACACAAAATTAGCTTGATGAGCTATAAACCAAGCTATTAGATTCATAAGTACATAAACTAAATATACATTATCTTAAAGGTTAGACATAACTTAATTGATAGGTTTGAATCTTCACTCCATGGAAAAAGAGAGAAAAATACTTAAGCTTACAGTTTAACTATTTTCTAAGATATGGGTAAACATTTATTCTGATAAAAGATAATTATAAATGTTATAATTGTTTGACTTGATGATGACAGTAATTAGCTCAGTCACTTCCTTCTTCCAAACTCACCTTCTATATGCAAAATCTAAAGCCAAGCATTTGACCAGGGGCTGGCAATATTGCTAATACAAGCAAAGATGCCCTCGCTGATGCCTCCTCCATGCGATCATCAAGCAAACCACGCTGCCGTGTGCGAAGAAGCAAGCGGTGGCAAAGCAGCAATGCTGTTCGCTGGGCTATATCTGGTGGCGTTGGGCGTCGGCGGGATAAAGGGGTCATTGCCATCGCATGGGGCCGAGCAGTTTGATGACAGTACCCCACAGGACAGGAAAAGAAGATCCACTTTCTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTTACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTCTTGTCCATCGTTGTCTTCTTTGCAGGCTCCCCTCTTTACCGCAACAAAATACCTAATGGAAGTCCATTCACAACAATTAGCAAGGTAATTATACCATAAAAATGAAGTTAAGAGTTAAAGAGTAAGGAATTTTGAGACAAAATGAAGTTAACTGAATCAGCTTGTGTTGTTTTGACTGAGCAGGTTTTAGTTGCTGCAATGTTTGGTTGCTGCATCAGCACGAACTCACGTAACTCGATAGCGAGTATGGCCATGAGCCCGGCTCTTGATGGCAAGGAAACTAAGCAGAATGCAAAAGGAAGAGATTCAACAGATGTGGTGGATGAACCATCAGAGAGTCTCAAGTTCCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCTGCATTAGAATGTACAGTTAAGGAAGTTGAAGAAGTCAAGATTGTGTTGAAAATTCTACCTATATTTGCTTGCACCATCATCCTCAACTGCTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCACTCCCCATATTCCCTATTCTCTTCATCATGTTCCTTGCTCCAATATACGATCACATAATCATCCCATTTTCTCGAAGATTAACTAAAACTGAAATGGGTATCACTCATCTCCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCTATGGCAGTTGCAGCAGCTGTCGAGACTAAGCGCAAGAGAGTAGCCACCGAAAACGGTCTACTTGACTCCGCTCAACCATTGCCCATCTCATTCCTTTGGATTGCCATCCAGTACTTGTTTCTTGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGCTCTGTGATCGTATCGATAGTCAACAATGTCACAGGTAGCTCCAACCACCGTCCATGGCTATCTGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGCGGATTAAATTTTGTGCACTACCTATTTTGGGCCATGAAGTACAAGTACAGACCAAAATCGCAACAATAG

mRNA sequence

ATGCAAAGGTGTGTGCGTCGTACTGGAGTATTCCCCCCTCCAATTACAAATATTTATTACCATGCAAAGGCAAATCACAATCTTGTTTACCTTGTTGTGTTCTTTCTTTACTCCTCTTCTGGTCTTGTCCCCATTCGTACGTTATTGGTCCTCAACCCCAATGAACATGTCCAATCAGCTCGCGCCACGCTGCTGACTCTTCTGTTTTGTGTTGGGACACGTGGCTCCAAGTCTCGATATGATATTGGGGGGCTGGCAATATTGCTAATACAAGCAAAGATGCCCTCGCTGATGCCTCCTCCATGCGATCATCAAGCAAACCACGCTGCCGTGTGCGAAGAAGCAAGCGGTGGCAAAGCAGCAATGCTGTTCGCTGGGCTATATCTGGTGGCGTTGGGCGTCGGCGGGATAAAGGGGTCATTGCCATCGCATGGGGCCGAGCAGTTTGATGACAGTACCCCACAGGACAGGAAAAGAAGATCCACTTTCTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTTACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTCTTGTCCATCGTTGTCTTCTTTGCAGGCTCCCCTCTTTACCGCAACAAAATACCTAATGGAAGTCCATTCACAACAATTAGCAAGGTTTTAGTTGCTGCAATGTTTGGTTGCTGCATCAGCACGAACTCACGTAACTCGATAGCGAGTATGGCCATGAGCCCGGCTCTTGATGGCAAGGAAACTAAGCAGAATGCAAAAGGAAGAGATTCAACAGATGTGGTGGATGAACCATCAGAGAGTCTCAAGTTCCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCTGCATTAGAATGTACAGTTAAGGAAGTTGAAGAAGTCAAGATTGTGTTGAAAATTCTACCTATATTTGCTTGCACCATCATCCTCAACTGCTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCACTCCCCATATTCCCTATTCTCTTCATCATGTTCCTTGCTCCAATATACGATCACATAATCATCCCATTTTCTCGAAGATTAACTAAAACTGAAATGGGTATCACTCATCTCCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCTATGGCAGTTGCAGCAGCTGTCGAGACTAAGCGCAAGAGAGTAGCCACCGAAAACGGTCTACTTGACTCCGCTCAACCATTGCCCATCTCATTCCTTTGGATTGCCATCCAGTACTTGTTTCTTGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGCTCTGTGATCGTATCGATAGTCAACAATGTCACAGGTAGCTCCAACCACCGTCCATGGCTATCTGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGCGGATTAAATTTTGTGCACTACCTATTTTGGGCCATGAAGTACAAGTACAGACCAAAATCGCAACAATAG

Coding sequence (CDS)

ATGCAAAGGTGTGTGCGTCGTACTGGAGTATTCCCCCCTCCAATTACAAATATTTATTACCATGCAAAGGCAAATCACAATCTTGTTTACCTTGTTGTGTTCTTTCTTTACTCCTCTTCTGGTCTTGTCCCCATTCGTACGTTATTGGTCCTCAACCCCAATGAACATGTCCAATCAGCTCGCGCCACGCTGCTGACTCTTCTGTTTTGTGTTGGGACACGTGGCTCCAAGTCTCGATATGATATTGGGGGGCTGGCAATATTGCTAATACAAGCAAAGATGCCCTCGCTGATGCCTCCTCCATGCGATCATCAAGCAAACCACGCTGCCGTGTGCGAAGAAGCAAGCGGTGGCAAAGCAGCAATGCTGTTCGCTGGGCTATATCTGGTGGCGTTGGGCGTCGGCGGGATAAAGGGGTCATTGCCATCGCATGGGGCCGAGCAGTTTGATGACAGTACCCCACAGGACAGGAAAAGAAGATCCACTTTCTTCAACTACTTCGTCTTTTGCCTCTCATGTGGTGGCCTAATTGCAGTTACATTGGTGGTGTGGGTTGAAGACAACAAAGGGTGGGAGTGGGGCTTTGGAATTGCAATGCTCAGCATATTCTTGTCCATCGTTGTCTTCTTTGCAGGCTCCCCTCTTTACCGCAACAAAATACCTAATGGAAGTCCATTCACAACAATTAGCAAGGTTTTAGTTGCTGCAATGTTTGGTTGCTGCATCAGCACGAACTCACGTAACTCGATAGCGAGTATGGCCATGAGCCCGGCTCTTGATGGCAAGGAAACTAAGCAGAATGCAAAAGGAAGAGATTCAACAGATGTGGTGGATGAACCATCAGAGAGTCTCAAGTTCCTAAATAATGCAGTTCTGAACAAGCCATTCCATTCTGCATTAGAATGTACAGTTAAGGAAGTTGAAGAAGTCAAGATTGTGTTGAAAATTCTACCTATATTTGCTTGCACCATCATCCTCAACTGCTGTGTAGCTCAGCTCTCCACATTTTCGGTTGAACAAGCATCAACAATGGACACCAAAATAGGTTCCTTTAAAGTTCCACCTGCATCACTCCCCATATTCCCTATTCTCTTCATCATGTTCCTTGCTCCAATATACGATCACATAATCATCCCATTTTCTCGAAGATTAACTAAAACTGAAATGGGTATCACTCATCTCCAGAGGATAGGAGTTGGGTTACTTTTCTCTATTGGAGCTATGGCAGTTGCAGCAGCTGTCGAGACTAAGCGCAAGAGAGTAGCCACCGAAAACGGTCTACTTGACTCCGCTCAACCATTGCCCATCTCATTCCTTTGGATTGCCATCCAGTACTTGTTTCTTGGATCAGCAGATCTTTTCTCCTTGGCTGGATCATTAGAATTTTTCTTCACAGAAGCACCTGCAAGCATGAGATCCTTGGCTACATCTCTCTCCTGGGCTTCTCTGGCTATGGGTTACTACCTTAGCTCTGTGATCGTATCGATAGTCAACAATGTCACAGGTAGCTCCAACCACCGTCCATGGCTATCTGGAAGAAACATAAACCATTATCACCTTGAGAGATTCTACTGGTTGATGTGTGTGCTCAGCGGATTAAATTTTGTGCACTACCTATTTTGGGCCATGAAGTACAAGTACAGACCAAAATCGCAACAATAG

Protein sequence

MQRCVRRTGVFPPPITNIYYHAKANHNLVYLVVFFLYSSSGLVPIRTLLVLNPNEHVQSARATLLTLLFCVGTRGSKSRYDIGGLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ
Homology
BLAST of Spg026056 vs. NCBI nr
Match: XP_022968372.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima])

HSP 1 Score: 884.8 bits (2285), Expect = 3.9e-253
Identity = 445/470 (94.68%), Postives = 458/470 (97.45%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLAIL+IQAKMPSL PPPCDHQANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 109 GLAILVIQAKMPSLKPPPCDHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPS 168

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDS PQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIF
Sbjct: 169 HGAEQFDDSNPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIF 228

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LS+VVFFAGSPLYRNKIPNGSPFTTISKVLVAA FGCC+S NSRNSIASMAMSPALDGKE
Sbjct: 229 LSMVVFFAGSPLYRNKIPNGSPFTTISKVLVAATFGCCVSPNSRNSIASMAMSPALDGKE 288

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
            KQNAKGRDSTDVVDEPSESLKFLNNAVL KPFHSALECTVKEVEEVKIVLKILPIFACT
Sbjct: 289 AKQNAKGRDSTDVVDEPSESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFACT 348

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHI+IPF+RR
Sbjct: 349 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIVIPFARR 408

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRKRVAT NGLLDSAQPLPI+FLWIAI
Sbjct: 409 ITKTEMGITHLQRIGVGLVFSIGAMAVAAAVETKRKRVATGNGLLDSAQPLPITFLWIAI 468

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VTG+
Sbjct: 469 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGN 528

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           SNH PWLSG NINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 529 SNHHPWLSGTNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of Spg026056 vs. NCBI nr
Match: XP_038893294.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 880.9 bits (2275), Expect = 5.6e-252
Identity = 448/471 (95.12%), Postives = 460/471 (97.66%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLA+LLIQAKMPSLMPPPCDHQANH AVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 111 GLAVLLIQAKMPSLMPPPCDHQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 170

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDSTPQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF
Sbjct: 171 HGAEQFDDSTPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 230

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VFFAGSPLYRNKIP+GSPFTTISKVLVAA FGCCISTNSRNSIASMAMSPALD KE
Sbjct: 231 LSIIVFFAGSPLYRNKIPDGSPFTTISKVLVAATFGCCISTNSRNSIASMAMSPALDDKE 290

Query: 264 TKQNAKGRDSTD-VVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFAC 323
            KQ AK R ST+ VVD+PSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVL+ILPIFAC
Sbjct: 291 AKQKAKERGSTNHVVDKPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFAC 350

Query: 324 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSR 383
           TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSR
Sbjct: 351 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSR 410

Query: 384 RLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIA 443
           R+TKTE GITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPI+FLWIA
Sbjct: 411 RITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIA 470

Query: 444 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTG 503
           IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVT 
Sbjct: 471 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTD 530

Query: 504 SSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           +S+H+PWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ
Sbjct: 531 NSDHKPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 581

BLAST of Spg026056 vs. NCBI nr
Match: XP_022944965.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 879.0 bits (2270), Expect = 2.1e-251
Identity = 442/470 (94.04%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLAILLIQAKMPSL PPPCDHQANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 109 GLAILLIQAKMPSLKPPPCDHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPS 168

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDS PQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIF
Sbjct: 169 HGAEQFDDSNPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIF 228

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LS+VVFFAGSPLYRNKIPNGSPFTTISKVLVAA F CC+S NS+NSIASMAMSPALDGKE
Sbjct: 229 LSMVVFFAGSPLYRNKIPNGSPFTTISKVLVAATFSCCVSPNSQNSIASMAMSPALDGKE 288

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
            KQNAKGRDSTDVVDEPSESLKFLNNAVL KPFHSALECTVKEVEEVKIVLKILPIF CT
Sbjct: 289 AKQNAKGRDSTDVVDEPSESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFGCT 348

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAP+YDHIIIPF+RR
Sbjct: 349 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPVYDHIIIPFARR 408

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRK VAT+NGLLDSAQPLPI+FLWIAI
Sbjct: 409 ITKTEMGITHLQRIGVGLVFSIGAMAVAAAVETKRKGVATKNGLLDSAQPLPITFLWIAI 468

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VTG+
Sbjct: 469 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGN 528

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           SNH PWLSG NINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 529 SNHHPWLSGTNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of Spg026056 vs. NCBI nr
Match: XP_023542267.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 876.3 bits (2263), Expect = 1.4e-250
Identity = 438/470 (93.19%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLA+LLIQAKMPSL PPPCDHQANH A+C+EASGG+AAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 109 GLAVLLIQAKMPSLKPPPCDHQANHTAMCQEASGGEAAMLFAGLYLVALGVGGIKGSLPS 168

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDSTPQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIF
Sbjct: 169 HGAEQFDDSTPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIF 228

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LS+VVFFAGSPLYRNKIPNGSPFTTISKVLVAA FGCC+S N+RNSIASMAMSPALDGKE
Sbjct: 229 LSMVVFFAGSPLYRNKIPNGSPFTTISKVLVAATFGCCVSPNTRNSIASMAMSPALDGKE 288

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
            KQNAKGRDSTDVVDEPSESLKFLNNAVL KPFHSALECTVKEVEEVKIVLKILPIF CT
Sbjct: 289 AKQNAKGRDSTDVVDEPSESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFGCT 348

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           IILNCCVAQLSTFSVEQASTM+TKIGSFKVPPASLPIFPILFIMFLAP+YDHIIIPF+RR
Sbjct: 349 IILNCCVAQLSTFSVEQASTMNTKIGSFKVPPASLPIFPILFIMFLAPVYDHIIIPFARR 408

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRK VAT+NGLLDS QPLPI+FLWIAI
Sbjct: 409 ITKTEMGITHLQRIGVGLVFSIGAMAVAAAVETKRKGVATKNGLLDSTQPLPITFLWIAI 468

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN +TG+
Sbjct: 469 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTITGN 528

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           SNH PWLSG NINHYHLERFYWLMCVL GLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 529 SNHHPWLSGTNINHYHLERFYWLMCVLGGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of Spg026056 vs. NCBI nr
Match: XP_011655668.1 (protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >ADQ74764.1 nitrate transporter 1.2a [Cucumis sativus] >KGN51883.1 hypothetical protein Csa_009160 [Cucumis sativus])

HSP 1 Score: 872.1 bits (2252), Expect = 2.6e-249
Identity = 443/471 (94.06%), Postives = 457/471 (97.03%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL +LLIQAKMPSLMPPPCD QANH AVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 113 GLTVLLIQAKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 172

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDSTPQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF
Sbjct: 173 HGAEQFDDSTPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 232

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VFFAGSPLYRNKIP+GSPFTTISKVLVAA FGCCI TNSRNSIASMAMSPALD KE
Sbjct: 233 LSIIVFFAGSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKE 292

Query: 264 TKQNAKGRDSTD-VVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFAC 323
            KQNAK  D+TD +++EPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVL+ILPIFAC
Sbjct: 293 AKQNAKEIDTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFAC 352

Query: 324 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSR 383
           TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSR
Sbjct: 353 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSR 412

Query: 384 RLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIA 443
           R+TKTE GITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPI+FLWIA
Sbjct: 413 RITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIA 472

Query: 444 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTG 503
           IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVT 
Sbjct: 473 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTD 532

Query: 504 SSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
            S+H+PWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 533 HSDHQPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 583

BLAST of Spg026056 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.7e-182
Identity = 323/475 (68.00%), Postives = 390/475 (82.11%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL IL IQA+ PSLMPP CD     +  CEE SG KAAMLF GLYLVALGVGGIKGSL S
Sbjct: 110 GLIILTIQARTPSLMPPSCD-----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLAS 169

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFD+STP+ RK+RSTFFNYFVFCL+CG L+AVT VVW+EDNKGWEWGFG++ ++IF
Sbjct: 170 HGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIF 229

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPA----- 263
           +SI++F +GS  YRNKIP GSP TTI KVL+AA   CC S +S N++ASM++SP+     
Sbjct: 230 VSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVS 289

Query: 264 -----LDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIV 323
                ++ +   +  +  ++     + + SLK LN A   KP H  LECTV++VE+VKIV
Sbjct: 290 KGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIV 349

Query: 324 LKILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIY 383
           LK+LPIFACTI+LNCC+AQLSTFSV+QA++M+TKIGS K+PPASLPIFP++FIM LAPIY
Sbjct: 350 LKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIY 409

Query: 384 DHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQP 443
           DH+IIPF+R+ TKTE G+THLQRIGVGL+ SI AMAVAA VE KRK VA ++GLLDS + 
Sbjct: 410 DHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKET 469

Query: 444 LPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVI 503
           LP++FLWIA+QYLFLGSADLF+LAG LE+FFTEAP+SMRSLATSLSWASLAMGYYLSSVI
Sbjct: 470 LPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVI 529

Query: 504 VSIVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYR 549
           VSIVN++TGSS + PWL G++IN Y L+ FYWLMCVLS  NF+HYLFWAM+YKYR
Sbjct: 530 VSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 579

BLAST of Spg026056 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.2e-166
Identity = 303/464 (65.30%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL +L IQA+ PSLMPPPC   A  A  CE   G KAA LF GLYLV+LG+GGIKGSLPS
Sbjct: 112 GLILLTIQARRPSLMPPPCKSSA--ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPS 171

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFD+ TP+ RK+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIF
Sbjct: 172 HGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIF 231

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VF  GS  Y+NKIP GSP TTI KVL+AA    C S  S N   S  +    + K 
Sbjct: 232 LSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT 291

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
             Q+             + SL  LN A+  K  H  LECTV++VE+VKIVLK+LPIF CT
Sbjct: 292 PSQSL------------TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCT 351

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           I+LNCC+AQLST+SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDH+IIPF+R+
Sbjct: 352 IMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARK 411

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TK+E+GITHLQRIGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPI+FLWIA+
Sbjct: 412 VTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIAL 471

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLF+LAG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT S
Sbjct: 472 QYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKS 531

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKY 548
           +   PWL G  +N   L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 532 AGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Spg026056 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 518.1 bits (1333), Expect = 1.2e-145
Identity = 266/432 (61.57%), Postives = 326/432 (75.46%), Query Frame = 0

Query: 123 LFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLV 182
           LF GLYLVALGVGGIKGSLP HGAEQFD+ T   R++RS FFNYF+F LSCG LIAVT+V
Sbjct: 162 LFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVV 221

Query: 183 VWVEDNKGWEWGFGIAMLSIFLSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCI 242
           VW+EDNKGW +GFG++  +I +S+ VF AGS +YR K+P+GSP TT+ KVL AA++    
Sbjct: 222 VWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYK 281

Query: 243 STNSRNSIASMAMSPALDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNK-PFHSALE 302
              +   + +       D   TKQN  G D             FL   V  +      L 
Sbjct: 282 KRRTSRIVVTCHTRNDCDDSVTKQNCDGDDGF--------LGSFLGEVVRERESLPRPLR 341

Query: 303 CTVKEVEEVKIVLKILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIF 362
           CT ++V++VKIV+KILPIF  TI+LNCC+AQLSTFSV+QASTM+TK+GSF VPPA+LP+F
Sbjct: 342 CTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVF 401

Query: 363 PILFIMFLAPIYDHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRV 422
           P++F+M LAP Y+H+++P +R+ TKTE GITHLQRIG GL+ SI AMAVAA VETKRK V
Sbjct: 402 PVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHV 461

Query: 423 -----ATENGLLDSAQPLPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLAT 482
                +  N    S+ PLPI+FLW+AIQY+FLGSADLF+LAG +EFFFTEAP++MRSLAT
Sbjct: 462 VVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLAT 521

Query: 483 SLSWASLAMGYYLSSVIVSIVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFV 542
           SLSWASLAMGYY SSV+VS VN VTG ++H PWL G N+N YHLERFYWLMCVLSG+NF+
Sbjct: 522 SLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFL 581

Query: 543 HYLFWAMKYKYR 549
           HYLFWA +Y YR
Sbjct: 582 HYLFWASRYVYR 585

BLAST of Spg026056 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.4e-85
Identity = 184/474 (38.82%), Postives = 287/474 (60.55%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           G++ L + A +P+L P  C         C  A+  + AM F GLYL+ALG GGIK  + S
Sbjct: 126 GMSALTLSASVPALKPAEC-----IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSS 185

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
            GA+QFDD+  ++R R+++FFN+F F ++ G L++ +L+VW+++N+GW  GFGI  + + 
Sbjct: 186 FGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMG 245

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSI--ASMAMSPALDG 263
           L+I  FF G+PLYR + P GSP T IS+V+VA+     +      ++   +   + A+ G
Sbjct: 246 LAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAG 305

Query: 264 K---ETKQNAKGRDSTDVV-DEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKIL 323
               E   + +  D   V+ +E S+S  + N+  L         CTV +VEE+KI++++ 
Sbjct: 306 SRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRL---------CTVTQVEELKILIRMF 365

Query: 324 PIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHII 383
           PI+A  II +   AQ+ST  V+Q   M+ KIGSF++PPA+L  F    ++   P+YD  I
Sbjct: 366 PIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFI 425

Query: 384 IPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPIS 443
           +P +R+ T  + G T +QR+G+GL  S+  MA AA VE  R  +A + GL++S  P+PIS
Sbjct: 426 VPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPIS 485

Query: 444 FLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIV 503
            LW   QY  LG+A++F   G LEFF+ ++P +MRSL ++L+  + A+G YLSS+I+++V
Sbjct: 486 VLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLV 545

Query: 504 NNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKS 552
              T  +    W+S  N+N  HL+ F+WL+  LS +N   Y F A +YK +  S
Sbjct: 546 TYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKAS 584

BLAST of Spg026056 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 7.9e-84
Identity = 185/475 (38.95%), Postives = 282/475 (59.37%), Query Frame = 0

Query: 81  DIGGLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGS 140
           ++ G  +L +QA +P L PP C+   +    CEEA G KA + F  LYLVALG G +K +
Sbjct: 123 ELSGFILLSVQAHLPQLKPPKCNPLIDQ--TCEEAKGFKAMIFFMALYLVALGSGCVKPN 182

Query: 141 LPSHGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAML 200
           + +HGA+QF  S P+  KR S++FN   F  S G LIA+TL+VWV+ + G + GFG++  
Sbjct: 183 MIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAA 242

Query: 201 SIFLSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALD 260
           ++ + I+   +G+  +RNK P  S FT I+ V+VAA+         +  +AS +    L 
Sbjct: 243 AMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAIL--------KRKLASPSDPRMLH 302

Query: 261 GKETKQNAKGRDSTDVVDEPSESL------KFLNNAVLNKPFHSALE-----CTVKEVEE 320
           G     N       DVV  PS +L      +FL+ A +     +  E     CTV +VE+
Sbjct: 303 GDHHVAN-------DVV--PSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQ 362

Query: 321 VKIVLKILPIFACTIILNCCVAQLSTFSVEQASTMDTKI-GSFKVPPASLPIFPILFIMF 380
           VK ++ ++PIFA TI+ N  +AQL TFSV+Q S+M+T++  SF +PPASL   P + ++F
Sbjct: 363 VKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIF 422

Query: 381 LAPIYDHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLL 440
           L P+YD  ++PF+R+LT    GI  L RIG+GL  S  +M  AA +E KR+  +  +G +
Sbjct: 423 LVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI 482

Query: 441 DSAQPLPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYY 500
                  +S  WI  Q+L  G +++F+  G +EFF+ ++   M S   +L++ S + G+Y
Sbjct: 483 -------LSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFY 542

Query: 501 LSSVIVSIVNNVTGSS-NHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWA 543
            SSV+VS+VN +T +S + + WL   ++N   L+ FYWL+ VLS LNF+ YLFW+
Sbjct: 543 FSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS 571

BLAST of Spg026056 vs. ExPASy TrEMBL
Match: A0A6J1HTC9 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111467635 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 1.9e-253
Identity = 445/470 (94.68%), Postives = 458/470 (97.45%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLAIL+IQAKMPSL PPPCDHQANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 109 GLAILVIQAKMPSLKPPPCDHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPS 168

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDS PQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIF
Sbjct: 169 HGAEQFDDSNPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIF 228

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LS+VVFFAGSPLYRNKIPNGSPFTTISKVLVAA FGCC+S NSRNSIASMAMSPALDGKE
Sbjct: 229 LSMVVFFAGSPLYRNKIPNGSPFTTISKVLVAATFGCCVSPNSRNSIASMAMSPALDGKE 288

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
            KQNAKGRDSTDVVDEPSESLKFLNNAVL KPFHSALECTVKEVEEVKIVLKILPIFACT
Sbjct: 289 AKQNAKGRDSTDVVDEPSESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFACT 348

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHI+IPF+RR
Sbjct: 349 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIVIPFARR 408

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRKRVAT NGLLDSAQPLPI+FLWIAI
Sbjct: 409 ITKTEMGITHLQRIGVGLVFSIGAMAVAAAVETKRKRVATGNGLLDSAQPLPITFLWIAI 468

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VTG+
Sbjct: 469 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGN 528

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           SNH PWLSG NINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 529 SNHHPWLSGTNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of Spg026056 vs. ExPASy TrEMBL
Match: A0A6J1FZJ7 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449346 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 1.0e-251
Identity = 442/470 (94.04%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GLAILLIQAKMPSL PPPCDHQANH A+C+EASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 109 GLAILLIQAKMPSLKPPPCDHQANHTAMCQEASGGKAAMLFAGLYLVALGVGGIKGSLPS 168

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDS PQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGI+MLSIF
Sbjct: 169 HGAEQFDDSNPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGISMLSIF 228

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LS+VVFFAGSPLYRNKIPNGSPFTTISKVLVAA F CC+S NS+NSIASMAMSPALDGKE
Sbjct: 229 LSMVVFFAGSPLYRNKIPNGSPFTTISKVLVAATFSCCVSPNSQNSIASMAMSPALDGKE 288

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
            KQNAKGRDSTDVVDEPSESLKFLNNAVL KPFHSALECTVKEVEEVKIVLKILPIF CT
Sbjct: 289 AKQNAKGRDSTDVVDEPSESLKFLNNAVLKKPFHSALECTVKEVEEVKIVLKILPIFGCT 348

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAP+YDHIIIPF+RR
Sbjct: 349 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPVYDHIIIPFARR 408

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRK VAT+NGLLDSAQPLPI+FLWIAI
Sbjct: 409 ITKTEMGITHLQRIGVGLVFSIGAMAVAAAVETKRKGVATKNGLLDSAQPLPITFLWIAI 468

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVN VTG+
Sbjct: 469 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNTVTGN 528

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           SNH PWLSG NINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 529 SNHHPWLSGTNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 578

BLAST of Spg026056 vs. ExPASy TrEMBL
Match: E5G6Y7 (Nitrate transporter 1.2a OS=Cucumis sativus OX=3659 GN=Csa_5G604280 PE=2 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.3e-249
Identity = 443/471 (94.06%), Postives = 457/471 (97.03%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL +LLIQAKMPSLMPPPCD QANH AVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS
Sbjct: 113 GLTVLLIQAKMPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 172

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFDDSTPQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF
Sbjct: 173 HGAEQFDDSTPQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 232

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VFFAGSPLYRNKIP+GSPFTTISKVLVAA FGCCI TNSRNSIASMAMSPALD KE
Sbjct: 233 LSIIVFFAGSPLYRNKIPDGSPFTTISKVLVAATFGCCIGTNSRNSIASMAMSPALDDKE 292

Query: 264 TKQNAKGRDSTD-VVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFAC 323
            KQNAK  D+TD +++EPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVL+ILPIFAC
Sbjct: 293 AKQNAKEIDTTDHMMNEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFAC 352

Query: 324 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSR 383
           TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSR
Sbjct: 353 TIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSR 412

Query: 384 RLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIA 443
           R+TKTE GITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPI+FLWIA
Sbjct: 413 RITKTETGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIA 472

Query: 444 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTG 503
           IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVT 
Sbjct: 473 IQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTD 532

Query: 504 SSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
            S+H+PWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 533 HSDHQPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 583

BLAST of Spg026056 vs. ExPASy TrEMBL
Match: A0A6J1DA77 (protein NRT1/ PTR FAMILY 4.6 OS=Momordica charantia OX=3673 GN=LOC111019092 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.4e-248
Identity = 439/478 (91.84%), Postives = 460/478 (96.23%), Query Frame = 0

Query: 76  SKSRYDIGGLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVG 135
           SKS+    GLAILLIQAK PSLMPPPCD    H AVC+EASGG+AAMLF GLYLVALGVG
Sbjct: 66  SKSQAFGQGLAILLIQAKSPSLMPPPCD----HGAVCQEASGGRAAMLFVGLYLVALGVG 125

Query: 136 GIKGSLPSHGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGF 195
           GIKGSLPSHGAEQFDDSTPQ RK+RSTFFNYFVFCLSCGGLIAVTLVVW+EDNKGWEWGF
Sbjct: 126 GIKGSLPSHGAEQFDDSTPQGRKKRSTFFNYFVFCLSCGGLIAVTLVVWLEDNKGWEWGF 185

Query: 196 GIAMLSIFLSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAM 255
           GIAMLSIFLSI+VFFAGSPLYRNK+PNGSPFTTISKVLVAA+FGCCISTNSRN IASMAM
Sbjct: 186 GIAMLSIFLSILVFFAGSPLYRNKVPNGSPFTTISKVLVAAIFGCCISTNSRNLIASMAM 245

Query: 256 SPALDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLK 315
           SPALDGKE KQNAKGRDSTD+ +EPS SLKFLNNAVLNKPFH ALECTV+EVEEVKIVLK
Sbjct: 246 SPALDGKEVKQNAKGRDSTDLANEPSGSLKFLNNAVLNKPFHPALECTVQEVEEVKIVLK 305

Query: 316 ILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH 375
           ILPIFACTIILNCCVAQLSTFSVEQASTMDTK+GSFKVPPASLPIFPILFIM LAP+YDH
Sbjct: 306 ILPIFACTIILNCCVAQLSTFSVEQASTMDTKLGSFKVPPASLPIFPILFIMLLAPVYDH 365

Query: 376 IIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLP 435
           IIIPFSRR+TKTEMGITHLQRIGVGL+FSIGAMAVAAAVETKRKRVAT+NGLLDSAQPLP
Sbjct: 366 IIIPFSRRITKTEMGITHLQRIGVGLIFSIGAMAVAAAVETKRKRVATDNGLLDSAQPLP 425

Query: 436 ISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVS 495
           ISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSV+VS
Sbjct: 426 ISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVVVS 485

Query: 496 IVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           IVNNVTG+S+HRPWLSGRNINHYHL+RFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 486 IVNNVTGNSDHRPWLSGRNINHYHLDRFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 539

BLAST of Spg026056 vs. ExPASy TrEMBL
Match: A0A5A7SYU8 (Protein NRT1/ PTR FAMILY 4.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00260 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.3e-246
Identity = 436/461 (94.58%), Postives = 451/461 (97.83%), Query Frame = 0

Query: 94  MPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDST 153
           MPSLMPPPCD QANH AVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDST
Sbjct: 1   MPSLMPPPCDRQANHNAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPSHGAEQFDDST 60

Query: 154 PQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIFLSIVVFFAGS 213
           PQ RKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLS+FLSI+VFFAGS
Sbjct: 61  PQGRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSLFLSIIVFFAGS 120

Query: 214 PLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKETKQNAKGRDS 273
           PLYRNKIP+GSPFTTISKVLVAA FGCCISTNSRNSIASMAMSPALD KE KQNAK RD+
Sbjct: 121 PLYRNKIPDGSPFTTISKVLVAATFGCCISTNSRNSIASMAMSPALDDKEAKQNAKERDA 180

Query: 274 TD-VVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACTIILNCCVAQ 333
           TD ++DEPS+SLKFLNNAVLNKPFHSALECTVKEVEEVKIVL+ILPIFACTIILNCCVAQ
Sbjct: 181 TDQMMDEPSQSLKFLNNAVLNKPFHSALECTVKEVEEVKIVLRILPIFACTIILNCCVAQ 240

Query: 334 LSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRRLTKTEMGIT 393
           LSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDH+IIPFSRR+TKTE GIT
Sbjct: 241 LSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHLIIPFSRRITKTETGIT 300

Query: 394 HLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAIQYLFLGSAD 453
           HLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPI+FLWIAIQYLFLGSAD
Sbjct: 301 HLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPITFLWIAIQYLFLGSAD 360

Query: 454 LFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGSSNHRPWLSG 513
           LFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVT +S+H+PWLSG
Sbjct: 361 LFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTDNSDHQPWLSG 420

Query: 514 RNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKSQQ 554
           RNINHYHLERFYWLMCVLSGLNFVHYLFWAM+YKYRPKSQQ
Sbjct: 421 RNINHYHLERFYWLMCVLSGLNFVHYLFWAMQYKYRPKSQQ 461

BLAST of Spg026056 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-183
Identity = 323/475 (68.00%), Postives = 390/475 (82.11%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL IL IQA+ PSLMPP CD     +  CEE SG KAAMLF GLYLVALGVGGIKGSL S
Sbjct: 110 GLIILTIQARTPSLMPPSCD-----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLAS 169

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFD+STP+ RK+RSTFFNYFVFCL+CG L+AVT VVW+EDNKGWEWGFG++ ++IF
Sbjct: 170 HGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIF 229

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPA----- 263
           +SI++F +GS  YRNKIP GSP TTI KVL+AA   CC S +S N++ASM++SP+     
Sbjct: 230 VSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVS 289

Query: 264 -----LDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIV 323
                ++ +   +  +  ++     + + SLK LN A   KP H  LECTV++VE+VKIV
Sbjct: 290 KGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIV 349

Query: 324 LKILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIY 383
           LK+LPIFACTI+LNCC+AQLSTFSV+QA++M+TKIGS K+PPASLPIFP++FIM LAPIY
Sbjct: 350 LKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIY 409

Query: 384 DHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQP 443
           DH+IIPF+R+ TKTE G+THLQRIGVGL+ SI AMAVAA VE KRK VA ++GLLDS + 
Sbjct: 410 DHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKET 469

Query: 444 LPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVI 503
           LP++FLWIA+QYLFLGSADLF+LAG LE+FFTEAP+SMRSLATSLSWASLAMGYYLSSVI
Sbjct: 470 LPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVI 529

Query: 504 VSIVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYR 549
           VSIVN++TGSS + PWL G++IN Y L+ FYWLMCVLS  NF+HYLFWAM+YKYR
Sbjct: 530 VSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 579

BLAST of Spg026056 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-167
Identity = 303/464 (65.30%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL +L IQA+ PSLMPPPC   A  A  CE   G KAA LF GLYLV+LG+GGIKGSLPS
Sbjct: 108 GLILLTIQARRPSLMPPPCKSSA--ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPS 167

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFD+ TP+ RK+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIF
Sbjct: 168 HGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIF 227

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VF  GS  Y+NKIP GSP TTI KVL+AA    C S  S N   S  +    + K 
Sbjct: 228 LSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT 287

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
             Q+             + SL  LN A+  K  H  LECTV++VE+VKIVLK+LPIF CT
Sbjct: 288 PSQSL------------TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCT 347

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           I+LNCC+AQLST+SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDH+IIPF+R+
Sbjct: 348 IMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARK 407

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TK+E+GITHLQRIGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPI+FLWIA+
Sbjct: 408 VTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIAL 467

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLF+LAG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT S
Sbjct: 468 QYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKS 527

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKY 548
           +   PWL G  +N   L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 528 AGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Spg026056 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.5e-167
Identity = 303/464 (65.30%), Postives = 360/464 (77.59%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           GL +L IQA+ PSLMPPPC   A  A  CE   G KAA LF GLYLV+LG+GGIKGSLPS
Sbjct: 112 GLILLTIQARRPSLMPPPCKSSA--ALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPS 171

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
           HGAEQFD+ TP+ RK+RSTFFNY+VFCLSCG L+AVT VVW+EDNKGWEWGFG++ +SIF
Sbjct: 172 HGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIF 231

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSIASMAMSPALDGKE 263
           LSI+VF  GS  Y+NKIP GSP TTI KVL+AA    C S  S N   S  +    + K 
Sbjct: 232 LSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT 291

Query: 264 TKQNAKGRDSTDVVDEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKILPIFACT 323
             Q+             + SL  LN A+  K  H  LECTV++VE+VKIVLK+LPIF CT
Sbjct: 292 PSQSL------------TNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCT 351

Query: 324 IILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHIIIPFSRR 383
           I+LNCC+AQLST+SV QA+TM+ KI +F VP ASLP+FP++F++ LAP YDH+IIPF+R+
Sbjct: 352 IMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARK 411

Query: 384 LTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPISFLWIAI 443
           +TK+E+GITHLQRIGVGL+ SI AMAVAA VE KRK+VA E GLLDS + LPI+FLWIA+
Sbjct: 412 VTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIAL 471

Query: 444 QYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIVNNVTGS 503
           QYLFLGSADLF+LAG LEFFFTEAP+SMRSLATSLSWASLA+GYYLSSV+V IVN VT S
Sbjct: 472 QYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKS 531

Query: 504 SNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKY 548
           +   PWL G  +N   L+ FYWLMCVLS +NF+HYLFWA +YKY
Sbjct: 532 AGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Spg026056 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 518.1 bits (1333), Expect = 8.8e-147
Identity = 266/432 (61.57%), Postives = 326/432 (75.46%), Query Frame = 0

Query: 123 LFAGLYLVALGVGGIKGSLPSHGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLV 182
           LF GLYLVALGVGGIKGSLP HGAEQFD+ T   R++RS FFNYF+F LSCG LIAVT+V
Sbjct: 162 LFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVV 221

Query: 183 VWVEDNKGWEWGFGIAMLSIFLSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCI 242
           VW+EDNKGW +GFG++  +I +S+ VF AGS +YR K+P+GSP TT+ KVL AA++    
Sbjct: 222 VWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYK 281

Query: 243 STNSRNSIASMAMSPALDGKETKQNAKGRDSTDVVDEPSESLKFLNNAVLNK-PFHSALE 302
              +   + +       D   TKQN  G D             FL   V  +      L 
Sbjct: 282 KRRTSRIVVTCHTRNDCDDSVTKQNCDGDDGF--------LGSFLGEVVRERESLPRPLR 341

Query: 303 CTVKEVEEVKIVLKILPIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIF 362
           CT ++V++VKIV+KILPIF  TI+LNCC+AQLSTFSV+QASTM+TK+GSF VPPA+LP+F
Sbjct: 342 CTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVF 401

Query: 363 PILFIMFLAPIYDHIIIPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRV 422
           P++F+M LAP Y+H+++P +R+ TKTE GITHLQRIG GL+ SI AMAVAA VETKRK V
Sbjct: 402 PVVFMMILAPTYNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHV 461

Query: 423 -----ATENGLLDSAQPLPISFLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLAT 482
                +  N    S+ PLPI+FLW+AIQY+FLGSADLF+LAG +EFFFTEAP++MRSLAT
Sbjct: 462 VVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLAT 521

Query: 483 SLSWASLAMGYYLSSVIVSIVNNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFV 542
           SLSWASLAMGYY SSV+VS VN VTG ++H PWL G N+N YHLERFYWLMCVLSG+NF+
Sbjct: 522 SLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFL 581

Query: 543 HYLFWAMKYKYR 549
           HYLFWA +Y YR
Sbjct: 582 HYLFWASRYVYR 585

BLAST of Spg026056 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 318.5 bits (815), Expect = 1.0e-86
Identity = 184/474 (38.82%), Postives = 287/474 (60.55%), Query Frame = 0

Query: 84  GLAILLIQAKMPSLMPPPCDHQANHAAVCEEASGGKAAMLFAGLYLVALGVGGIKGSLPS 143
           G++ L + A +P+L P  C         C  A+  + AM F GLYL+ALG GGIK  + S
Sbjct: 126 GMSALTLSASVPALKPAEC-----IGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSS 185

Query: 144 HGAEQFDDSTPQDRKRRSTFFNYFVFCLSCGGLIAVTLVVWVEDNKGWEWGFGIAMLSIF 203
            GA+QFDD+  ++R R+++FFN+F F ++ G L++ +L+VW+++N+GW  GFGI  + + 
Sbjct: 186 FGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMG 245

Query: 204 LSIVVFFAGSPLYRNKIPNGSPFTTISKVLVAAMFGCCISTNSRNSI--ASMAMSPALDG 263
           L+I  FF G+PLYR + P GSP T IS+V+VA+     +      ++   +   + A+ G
Sbjct: 246 LAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAG 305

Query: 264 K---ETKQNAKGRDSTDVV-DEPSESLKFLNNAVLNKPFHSALECTVKEVEEVKIVLKIL 323
               E   + +  D   V+ +E S+S  + N+  L         CTV +VEE+KI++++ 
Sbjct: 306 SRKIEHTDDCQYLDKAAVISEEESKSGDYSNSWRL---------CTVTQVEELKILIRMF 365

Query: 324 PIFACTIILNCCVAQLSTFSVEQASTMDTKIGSFKVPPASLPIFPILFIMFLAPIYDHII 383
           PI+A  II +   AQ+ST  V+Q   M+ KIGSF++PPA+L  F    ++   P+YD  I
Sbjct: 366 PIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLYDRFI 425

Query: 384 IPFSRRLTKTEMGITHLQRIGVGLLFSIGAMAVAAAVETKRKRVATENGLLDSAQPLPIS 443
           +P +R+ T  + G T +QR+G+GL  S+  MA AA VE  R  +A + GL++S  P+PIS
Sbjct: 426 VPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPIS 485

Query: 444 FLWIAIQYLFLGSADLFSLAGSLEFFFTEAPASMRSLATSLSWASLAMGYYLSSVIVSIV 503
            LW   QY  LG+A++F   G LEFF+ ++P +MRSL ++L+  + A+G YLSS+I+++V
Sbjct: 486 VLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLV 545

Query: 504 NNVTGSSNHRPWLSGRNINHYHLERFYWLMCVLSGLNFVHYLFWAMKYKYRPKS 552
              T  +    W+S  N+N  HL+ F+WL+  LS +N   Y F A +YK +  S
Sbjct: 546 TYFTTRNGQEGWISD-NLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKKAS 584

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022968372.13.9e-25394.68protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima][more]
XP_038893294.15.6e-25295.12protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_022944965.12.1e-25194.04protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_023542267.11.4e-25093.19protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
XP_011655668.12.6e-24994.06protein NRT1/ PTR FAMILY 4.6 [Cucumis sativus] >ADQ74764.1 nitrate transporter 1... [more]
Match NameE-valueIdentityDescription
Q8H1571.7e-18268.00Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.2e-16665.30Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM201.2e-14561.57Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
P460321.4e-8538.82Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q93VV57.9e-8438.95Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HTC91.9e-25394.68protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111467635 PE... [more]
A0A6J1FZJ71.0e-25194.04protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449346 ... [more]
E5G6Y71.3e-24994.06Nitrate transporter 1.2a OS=Cucumis sativus OX=3659 GN=Csa_5G604280 PE=2 SV=1[more]
A0A6J1DA771.4e-24891.84protein NRT1/ PTR FAMILY 4.6 OS=Momordica charantia OX=3673 GN=LOC111019092 PE=3... [more]
A0A5A7SYU81.3e-24694.58Protein NRT1/ PTR FAMILY 4.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
AT1G69850.11.2e-18368.00nitrate transporter 1:2 [more]
AT1G27040.21.5e-16765.30Major facilitator superfamily protein [more]
AT1G27040.11.5e-16765.30Major facilitator superfamily protein [more]
AT5G62730.18.8e-14761.57Major facilitator superfamily protein [more]
AT2G02040.11.0e-8638.82peptide transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 84..509
e-value: 8.4E-105
score: 350.8
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 81..550
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 60..553
e-value: 1.5E-141
score: 474.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 120..549
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 81..550

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg026056.1Spg026056.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity