Spg025927 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025927 vs. NCBI nr
Match: XP_038893145.1 (protein DETOXIFICATION 29 [Benincasa hispida])

HSP 1 Score: 840.9 bits (2171), Expect = 5.7e-240
Identity = 433/505 (85.74%), Postives = 464/505 (91.88%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MAD SQPLL+ RE+NK+I  PESGR+ TK VVFAPDADDIPPIN ARDFYREFCVE KKL
Sbjct: 1   MADFSQPLLSQREENKQIHQPESGRKGTK-VVFAPDADDIPPINTARDFYREFCVELKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENSVIAGFSFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDMMGVYMQRSWVILLS+AVILTPIYIFA PLLKLIGQTAE+SEAAG+MS
Sbjct: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSSAVILTPIYIFATPLLKLIGQTAEISEAAGVMS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP +KFLQAQSKMMAMSVISAVAL  H  F+WLLMLKLG GL GGA+
Sbjct: 181 IWMIPQLYAYALNFPISKFLQAQSKMMAMSVISAVALALHTFFTWLLMLKLGLGLAGGAI 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLN SW VIVVAQI+YI+SGSCG AW+GFSWRAFQSLWGFV+LSLASA+M+CLE WYFMA
Sbjct: 241 VLNVSWWVIVVAQIIYIISGSCGRAWSGFSWRAFQSLWGFVRLSLASAIMLCLEIWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSID LSICTNI+GWTVMV FGINAAISVRVSNELGAAHPRTARFSLV
Sbjct: 301 LILFAGYLKNAEVSIDGLSICTNILGWTVMVAFGINAAISVRVSNELGAAHPRTARFSLV 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AAIL++TKNDYP LFSSDSAVRQ+V NLTPLL FCIL+NNVQPVLSG
Sbjct: 361 VAVISSFVLGLILAAILIITKNDYPFLFSSDSAVRQVVMNLTPLLGFCILVNNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVN+GCYY+FGVP GLLMGF LHWGVTGIWSGMI GT+IQTCILT++V
Sbjct: 421 VAVGAGWQAVVAYVNVGCYYLFGVPLGLLMGFTLHWGVTGIWSGMIGGTIIQTCILTWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           Y+TNWN+EASVAEDRIRKWGG S S
Sbjct: 481 YKTNWNEEASVAEDRIRKWGGPSVS 504

BLAST of Spg025927 vs. NCBI nr
Match: XP_004135268.1 (protein DETOXIFICATION 29 [Cucumis sativus] >KGN51764.1 hypothetical protein Csa_008817 [Cucumis sativus])

HSP 1 Score: 821.2 bits (2120), Expect = 4.7e-234
Identity = 415/501 (82.83%), Postives = 461/501 (92.02%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MA+LSQPLL+  E+NK IDSPESGR++TK V+FAPDADDIPPIN ARDFYREFC+E KKL
Sbjct: 1   MAELSQPLLSQSEENKLIDSPESGRKDTK-VLFAPDADDIPPINTARDFYREFCIELKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTS+CQYSFGAITQLFAGQVSTIALAAVS+ENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSICQYSFGAITQLFAGQVSTIALAAVSVENSVIAGFSFGIMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQL MMGVY+QRSWVILL+TAV+LTPIYIF+APLLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLGMMGVYLQRSWVILLTTAVVLTPIYIFSAPLLKLIGQTAEISEAAGVLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP +KFLQAQSKMMAMSVISAVALVFH  F+WL MLKLGWGL GGA+
Sbjct: 181 IWMIPQLYAYALNFPVSKFLQAQSKMMAMSVISAVALVFHTFFTWLFMLKLGWGLAGGAI 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW VI  AQIVYILSGSCG AW+GFSW+AF +LWGFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWVIDFAQIVYILSGSCGRAWSGFSWQAFHNLWGFVRLSLASAVMLCLEIWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSIDALSICTNI+GWTVMV FGINAAISVRVSNELGAAHPRTARFSLV
Sbjct: 301 LILFAGYLKNAEVSIDALSICTNILGWTVMVAFGINAAISVRVSNELGAAHPRTARFSLV 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAV SSFV+GLI  AIL++TK+DYP LFS+DSAVRQIVKNLTP+L FCI++NN+QPVLSG
Sbjct: 361 VAVASSFVVGLILTAILIITKDDYPYLFSNDSAVRQIVKNLTPMLGFCIVVNNIQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVN+GCYY+FG+P GLL+GF LHWGV GIWSGMI GT+IQT IL ++V
Sbjct: 421 VAVGAGWQAVVAYVNVGCYYLFGIPLGLLLGFALHWGVLGIWSGMIGGTIIQTFILVWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGG 492
           Y+TNWN+EASVAEDRIRKWGG
Sbjct: 481 YKTNWNEEASVAEDRIRKWGG 500

BLAST of Spg025927 vs. NCBI nr
Match: XP_023550408.1 (LOW QUALITY PROTEIN: protein DETOXIFICATION 29-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 808.5 bits (2087), Expect = 3.1e-230
Identity = 420/505 (83.17%), Postives = 456/505 (90.30%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADLSQPLL P E+ K I SPESGR+ETKA  F PD  DIPPINGARDF REF VEFKKL
Sbjct: 1   MADLSQPLLLPTEETKWIHSPESGRKETKA-KFVPDGGDIPPINGARDFNREFYVEFKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFT +CQYSFGAITQLFAGQVSTIALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTFICQYSFGAITQLFAGQVSTIALAAVSIENSVIAGFSFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTP+YIF+A LLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPVYIFSAALLKLIGQTAEISEAAGMLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP ++FLQAQSKMMAMSVISAVALV H  F+WLLM+KLGWGL GGAV
Sbjct: 181 IWMIPQLYAYALNFPVSRFLQAQSKMMAMSVISAVALVLHTFFTWLLMVKLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW +IVVAQIVYILSGSCG AW+GFSWRAFQSL GFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWLIVVAQIVYILSGSCGRAWSGFSWRAFQSLSGFVRLSLASAVMLCLETWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSI+ALSICTNI+GWT+MV FGINAAISVRVSNELGA HPRTARFSL+
Sbjct: 301 LILFAGYLKNAEVSINALSICTNILGWTMMVAFGINAAISVRVSNELGAGHPRTARFSLI 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AA+L++TKNDYP +FSSDSAVRQIVK+LT  L FCI++NNVQPVLSG
Sbjct: 361 VAVISSFVLGLIMAAVLVITKNDYPFIFSSDSAVRQIVKDLTLWLGFCIMVNNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA VAYVN+GCYY FGVP GLLMGFML WGVTGIWSGMI GT++QTCIL ++V
Sbjct: 421 VAVGAGWQAAVAYVNVGCYYAFGVPLGLLMGFMLDWGVTGIWSGMIGGTILQTCILIWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           +RTNW+ EASVAEDRIRKWGG S S
Sbjct: 481 HRTNWDTEASVAEDRIRKWGGPSIS 504

BLAST of Spg025927 vs. NCBI nr
Match: XP_022957306.1 (protein DETOXIFICATION 29-like [Cucurbita moschata])

HSP 1 Score: 807.7 bits (2085), Expect = 5.4e-230
Identity = 417/505 (82.57%), Postives = 456/505 (90.30%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADLSQPLL+P E+ K I SPESGR+ETKA  F PD  DIPPINGARDFYREF VEFKKL
Sbjct: 1   MADLSQPLLSPTEETKWIHSPESGRKETKA-KFVPDGGDIPPINGARDFYREFYVEFKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFT +CQYSFGAITQLFAGQVSTIALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTFICQYSFGAITQLFAGQVSTIALAAVSIENSVIAGFSFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDMMGVYMQRSWVILLSTA+ILTP+YIF+ PLLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTALILTPVYIFSVPLLKLIGQTAEISEAAGVLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP ++FLQAQSKMMAMSVISAVALV H  F+WLLM++LGWGL GGAV
Sbjct: 181 IWMIPQLYAYALNFPVSRFLQAQSKMMAMSVISAVALVLHTFFTWLLMVRLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW +IVVAQIVYILSGSCG AW+GFSWRAFQSL GFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWLIVVAQIVYILSGSCGRAWSGFSWRAFQSLSGFVRLSLASAVMLCLETWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSI+ALSICTN++GWT+MV FGINAAISVRVSNELGA HPRTARFSL+
Sbjct: 301 LILFAGYLKNAEVSINALSICTNVLGWTMMVAFGINAAISVRVSNELGAGHPRTARFSLI 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AA+L++TKN YP +FSSDSAVRQIVK+LT  L FCI++NNVQPVLSG
Sbjct: 361 VAVISSFVLGLIMAAVLIITKNSYPFIFSSDSAVRQIVKDLTLWLGFCIMVNNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA VAYVN+GCYY FGVP GLLMGFML WGVTGIW+GMI GT+IQTCIL ++V
Sbjct: 421 VAVGAGWQAAVAYVNVGCYYAFGVPLGLLMGFMLDWGVTGIWAGMIGGTIIQTCILIWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
            RTNW+ EASVAEDRIRKWGG S S
Sbjct: 481 QRTNWDTEASVAEDRIRKWGGPSIS 504

BLAST of Spg025927 vs. NCBI nr
Match: XP_022151982.1 (protein DETOXIFICATION 30 [Momordica charantia])

HSP 1 Score: 806.2 bits (2081), Expect = 1.6e-229
Identity = 408/505 (80.79%), Postives = 454/505 (89.90%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADL+Q LL+PR++ KR  SPES +RETKA  F+P ADDIPPIN  RDFYREFCVE +KL
Sbjct: 1   MADLTQQLLSPRDEEKRSHSPESRKRETKAATFSPGADDIPPINSVRDFYREFCVEVRKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTS+CQYSFGAITQL AGQVSTIALAAVS+ENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSICQYSFGAITQLLAGQVSTIALAAVSVENSVIAGFSLGIMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDM+G+YMQRSW+ILL+TAVI+TP Y+FAAPLLKL GQT  VSEAAG+MS
Sbjct: 121 LCGQAYGAGQLDMLGIYMQRSWIILLTTAVIMTPAYVFAAPLLKLTGQTEAVSEAAGVMS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYA+NFP +KFLQAQSKMMAMS I+AVALV H  FSWLLMLKLGWGL GGAV
Sbjct: 181 VWMIPQLYAYAVNFPISKFLQAQSKMMAMSAIAAVALVLHAFFSWLLMLKLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNA+W  +VVAQ+VYI  GSCG AW+GFSWRAFQSLWGFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNATWWFVVVAQLVYIFMGSCGRAWSGFSWRAFQSLWGFVRLSLASAVMLCLEIWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           LVLFAGYL++AEVSIDALSICTNI+GW VMV FGINAA+SVR+SNELGAAHPRTARFSLV
Sbjct: 301 LVLFAGYLEDAEVSIDALSICTNILGWMVMVAFGINAAVSVRISNELGAAHPRTARFSLV 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISS +LGL+ A+ILL+TKNDYPSLFS+DSAVRQIVK LTP+LCFCI+INNVQPVLSG
Sbjct: 361 VAVISSCLLGLMLASILLITKNDYPSLFSNDSAVRQIVKKLTPMLCFCIVINNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVNIGCYYVFGVP GLLMG+   +GV+GIWSGM+ GT+IQTCILT ++
Sbjct: 421 VAVGAGWQAVVAYVNIGCYYVFGVPLGLLMGYKFDFGVSGIWSGMMCGTIIQTCILTGMI 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           YRTNWNKEASVAEDRIRKWGG S S
Sbjct: 481 YRTNWNKEASVAEDRIRKWGGDSIS 505

BLAST of Spg025927 vs. ExPASy Swiss-Prot
Match: Q9LPV4 (Protein DETOXIFICATION 31 OS=Arabidopsis thaliana OX=3702 GN=DTX31 PE=2 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.5e-179
Identity = 321/501 (64.07%), Postives = 396/501 (79.04%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           +A   +  L P  Q   ++    G R +  V F+  A DIPPI+G  DF REF +E +KL
Sbjct: 12  LASTEEEELDPATQKALMEYLGVGSRASSLVSFSSTAVDIPPISGVGDFVREFRIESRKL 71

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           W LA PA+FT++ QYS GA+TQ+FAG +ST+ALAAVSIENS          LGMGSALET
Sbjct: 72  WKLAGPAIFTTMSQYSLGAVTQVFAGHISTLALAAVSIENSVIAGFSFGIMLGMGSALET 131

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQA+GAG++ M+GVY+QRSWVIL  TA+ L+ IYIFAAP+L  IGQTA +S  AG+ S
Sbjct: 132 LCGQAFGAGKVSMLGVYLQRSWVILSVTALFLSLIYIFAAPILTFIGQTAAISAMAGIFS 191

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           ++MIPQ++AYA+NFPTAKFLQ+QSK+M M+ IS V LV H  F+WL+M +L WGL G A+
Sbjct: 192 IYMIPQIFAYAINFPTAKFLQSQSKIMVMAGISGVVLVIHSFFTWLVMSRLHWGLPGLAL 251

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLN SW VIVVAQ+VYI + +CG AW+GF+W AF +LWGFVKLSLASA M+CLE WYFMA
Sbjct: 252 VLNTSWWVIVVAQLVYIFNCTCGEAWSGFTWEAFHNLWGFVKLSLASAAMLCLEIWYFMA 311

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           LVLFAGYLKNAEVS+ ALSIC NI+GW  MV FG NAA+SVRVSNELGA+HPRTA+FSLV
Sbjct: 312 LVLFAGYLKNAEVSVAALSICMNILGWAAMVAFGTNAAVSVRVSNELGASHPRTAKFSLV 371

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVI S  +G+  AA LL  +N+YP LF  D  VR +V+ LTP+L FCI+INNVQPVLSG
Sbjct: 372 VAVILSTAIGMFIAAGLLFFRNEYPVLFVEDEEVRNVVRELTPMLAFCIVINNVQPVLSG 431

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVNI CYY+FGVPFGLL+GF L +GV GIW GM++GT +Q+ +LT+++
Sbjct: 432 VAVGAGWQAVVAYVNIACYYLFGVPFGLLLGFKLEYGVMGIWWGMVTGTFVQSIVLTWMI 491

Query: 481 YRTNWNKEASVAEDRIRKWGG 492
            +TNW KEAS+AE+RI++WGG
Sbjct: 492 CKTNWEKEASMAEERIKEWGG 512

BLAST of Spg025927 vs. ExPASy Swiss-Prot
Match: Q38956 (Protein DETOXIFICATION 29 OS=Arabidopsis thaliana OX=3702 GN=DTX29 PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 7.6e-179
Identity = 315/464 (67.89%), Postives = 387/464 (83.41%), Query Frame = 0

Query: 38  DDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVS 97
           DDIPPI     F REF VE KKLWYLA PA+FTSV QYS GAITQ+FAG +STIALAAVS
Sbjct: 27  DDIPPITTVGGFVREFNVETKKLWYLAGPAIFTSVNQYSLGAITQVFAGHISTIALAAVS 86

Query: 98  IENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYI 157
           +ENS          LGMGSALETLCGQA+GAG+L M+GVY+QRSWVIL  TA+IL+ +YI
Sbjct: 87  VENSVVAGFSFGIMLGMGSALETLCGQAFGAGKLSMLGVYLQRSWVILNVTALILSLLYI 146

Query: 158 FAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVAL 217
           FAAP+L  IGQTA +S AAG+ S++MIPQ++AYA+NFPTAKFLQ+QSK+M M+VISAVAL
Sbjct: 147 FAAPILASIGQTAAISSAAGIFSIYMIPQIFAYAINFPTAKFLQSQSKIMVMAVISAVAL 206

Query: 218 VFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSL 277
           V HV  +W +++KL WG+ G AVVLNASWC I +AQ+VYI SG+CG AW+GFSW AF +L
Sbjct: 207 VIHVPLTWFVIVKLQWGMPGLAVVLNASWCFIDMAQLVYIFSGTCGEAWSGFSWEAFHNL 266

Query: 278 WGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINA 337
           W FV+LSLASAVM+CLE WYFMA++LFAGYLKNAE+S+ ALSIC NI+GWT M+  G+N 
Sbjct: 267 WSFVRLSLASAVMLCLEVWYFMAIILFAGYLKNAEISVAALSICMNILGWTAMIAIGMNT 326

Query: 338 AISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQI 397
           A+SVRVSNELGA HPRTA+FSL+VAVI+S ++G I + ILL+ ++ YPSLF  D  V  +
Sbjct: 327 AVSVRVSNELGANHPRTAKFSLLVAVITSTLIGFIVSMILLIFRDQYPSLFVKDEKVIIL 386

Query: 398 VKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWG 457
           VK LTP+L   I+INNVQPVLSGVAVGAGWQA+VAYVNI CYYVFG+PFGLL+G+ L++G
Sbjct: 387 VKELTPILALSIVINNVQPVLSGVAVGAGWQAVVAYVNIACYYVFGIPFGLLLGYKLNYG 446

Query: 458 VTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           V GIW GM++GT++QT +LT+++ +TNW+ EAS+AEDRIR+WGG
Sbjct: 447 VMGIWCGMLTGTVVQTIVLTWMICKTNWDTEASMAEDRIREWGG 490

BLAST of Spg025927 vs. ExPASy Swiss-Prot
Match: Q9LS19 (Protein DETOXIFICATION 30 OS=Arabidopsis thaliana OX=3702 GN=DTX30 PE=2 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 9.7e-174
Identity = 311/479 (64.93%), Postives = 385/479 (80.38%), Query Frame = 0

Query: 23  SGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQ 82
           S   E  A+ F+   +DIPPI     F +EF VE KKLWYLA PA+F S+ QYS GA TQ
Sbjct: 13  SAAEEPPALPFS-SVEDIPPITTVGGFVKEFNVEVKKLWYLAGPAIFMSITQYSLGAATQ 72

Query: 83  LFAGQVSTIALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSW 142
           +FAG +STIALAAVS+ENS          LGMGSALETLCGQA+GAG+L M+GVY+QRSW
Sbjct: 73  VFAGHISTIALAAVSVENSVIAGFSFGVMLGMGSALETLCGQAFGAGKLSMLGVYLQRSW 132

Query: 143 VILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQA 202
           VIL  TAVIL+ +YIFAAP+L  IGQT  +S A G+ S++MIPQ++AYA+N+PTAKFLQ+
Sbjct: 133 VILNVTAVILSLLYIFAAPILAFIGQTPAISSATGIFSIYMIPQIFAYAVNYPTAKFLQS 192

Query: 203 QSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSC 262
           QSK+M M+ ISAVALV HVL +W ++  L WG  G AVVLNASW  IVVAQ+VYI SG+C
Sbjct: 193 QSKIMVMAAISAVALVLHVLLTWFVIEGLQWGTAGLAVVLNASWWFIVVAQLVYIFSGTC 252

Query: 263 GPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICT 322
           G AW+GFSW AF +LW FV+LSLASAVM+CLE WY MA++LFAGYLKNAE+S+ ALSIC 
Sbjct: 253 GEAWSGFSWEAFHNLWSFVRLSLASAVMLCLEVWYLMAVILFAGYLKNAEISVAALSICM 312

Query: 323 NIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKN 382
           NI+GWT M+  G+NAA+SVRVSNELGA HPRTA+FSL+VAVI+S V+GL  +  LL+ ++
Sbjct: 313 NILGWTAMIAIGMNAAVSVRVSNELGAKHPRTAKFSLLVAVITSTVIGLAISIALLIFRD 372

Query: 383 DYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVF 442
            YPSLF  D  V  +VK+LTP+L   I+INNVQPVLSGVAVGAGWQA+VAYVNI CYYVF
Sbjct: 373 KYPSLFVGDEEVIIVVKDLTPILAVSIVINNVQPVLSGVAVGAGWQAVVAYVNIVCYYVF 432

Query: 443 GVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           G+PFGLL+G+ L++GV GIW GM++GT++QT +LT+++ RTNW+ EA++AE RIR+WGG
Sbjct: 433 GIPFGLLLGYKLNFGVMGIWCGMLTGTVVQTIVLTWMICRTNWDTEAAMAEGRIREWGG 490

BLAST of Spg025927 vs. ExPASy Swiss-Prot
Match: F4I4Q3 (Protein DETOXIFICATION 32 OS=Arabidopsis thaliana OX=3702 GN=DTX32 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 4.8e-173
Identity = 308/487 (63.24%), Postives = 383/487 (78.64%), Query Frame = 0

Query: 18  IDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSF 77
           I S +  R  TK+        D+PPI+G RDF R+F  E KKLW+LA PA+FTS CQYS 
Sbjct: 12  ISSEQEHRAHTKS------DTDMPPISGGRDFIRQFAAESKKLWWLAGPAIFTSFCQYSL 71

Query: 78  GAITQLFAGQVSTIALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVY 137
           GA+TQ+ AG V+T+ALAAVSI+NS          LGMGSAL TLCGQAYGAGQL+MMG+Y
Sbjct: 72  GAVTQILAGHVNTLALAAVSIQNSVISGFSVGIMLGMGSALATLCGQAYGAGQLEMMGIY 131

Query: 138 MQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTA 197
           +QRSW+IL S A++L   Y+FA PLL L+GQ+ E+S+AAG  SLWMIPQL+AYA+NF TA
Sbjct: 132 LQRSWIILNSCALLLCLFYVFATPLLSLLGQSPEISKAAGKFSLWMIPQLFAYAVNFATA 191

Query: 198 KFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYI 257
           KFLQAQSK++AM+VI+A  L+ H L SWLLMLKL WG+ GGAVVLN SW +I V QIVYI
Sbjct: 192 KFLQAQSKVIAMAVIAATVLLQHTLLSWLLMLKLRWGMAGGAVVLNMSWWLIDVTQIVYI 251

Query: 258 LSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDA 317
             GS G AW+G SW AF++L GF +LSLASAVM+CLE WYFMAL+LFAGYLKN +VS+ A
Sbjct: 252 CGGSSGRAWSGLSWMAFKNLRGFARLSLASAVMVCLEVWYFMALILFAGYLKNPQVSVAA 311

Query: 318 LSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAIL 377
           LSIC NI+GW +MV FG NAA+SVR SNELGA HPR A+F L+VA+I+S  +G++ +  L
Sbjct: 312 LSICMNILGWPIMVAFGFNAAVSVRESNELGAEHPRRAKFLLIVAMITSVSIGIVISVTL 371

Query: 378 LVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIG 437
           +V ++ YP++FS D  VR +VK LTPLL   I+INN+QPVLSGVAVGAGWQ IVAYVNIG
Sbjct: 372 IVLRDKYPAMFSDDEEVRVLVKQLTPLLALTIVINNIQPVLSGVAVGAGWQGIVAYVNIG 431

Query: 438 CYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIR 495
           CYY+ G+P GL++G+ +  GV GIW+GM++GT++QT +L FI+YRTNW KEAS+AE RI+
Sbjct: 432 CYYLCGIPIGLVLGYKMELGVKGIWTGMLTGTVVQTSVLLFIIYRTNWKKEASLAEARIK 491

BLAST of Spg025927 vs. ExPASy Swiss-Prot
Match: Q9SX83 (Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.7e-142
Identity = 259/470 (55.11%), Postives = 338/470 (71.91%), Query Frame = 0

Query: 36  DADDIPPINGARD----FYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQLFAGQVSTI 95
           D  + P + G +     + +EF  E K+LW LA PA+FT++ QYS GA+TQ F+G++  +
Sbjct: 11  DPREPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGEL 70

Query: 96  ALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSWVILLSTAVI 155
            LAAVS+ENS          LGMGSALETLCGQAYGAGQ+ MMG+YMQRSWVIL +TA+ 
Sbjct: 71  ELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALF 130

Query: 156 LTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQAQSKMMAMSV 215
           L P+YI+A P+L   G+   +S+AAG  +LWMIPQL+AYA NFP  KFLQ+Q K++ M+ 
Sbjct: 131 LLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAW 190

Query: 216 ISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSCGPAWTGFSW 275
           IS V LV H +FSWL +L   WGL G A+ LN SW +IV+ Q++YIL      AWTGFS 
Sbjct: 191 ISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSM 250

Query: 276 RAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICTNIVGWTVMV 335
            AF+ L+GFVKLSLASA+M+CLE WY M LV+  G L N  + +DA+SIC NI GWT M+
Sbjct: 251 LAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMI 310

Query: 336 TFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKNDYPSLFSSD 395
           + G NAAISVRVSNELGA +   A+FS++V  I+S ++G++   ++L TK+ +P LF+S 
Sbjct: 311 SIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSS 370

Query: 396 SAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVFGVPFGLLMG 455
            AV      +  LL F +L+N++QPVLSGVAVGAGWQA+VAYVNI CYY+ G+P GL++G
Sbjct: 371 EAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLG 430

Query: 456 FMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           F L  GV GIW GM++G  +QT IL  I+Y TNWNKEA  AE R+++WGG
Sbjct: 431 FTLDLGVQGIWGGMVAGICLQTLILIGIIYFTNWNKEAEQAESRVQRWGG 480

BLAST of Spg025927 vs. ExPASy TrEMBL
Match: A0A0A0KS80 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G598720 PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 2.3e-234
Identity = 415/501 (82.83%), Postives = 461/501 (92.02%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MA+LSQPLL+  E+NK IDSPESGR++TK V+FAPDADDIPPIN ARDFYREFC+E KKL
Sbjct: 1   MAELSQPLLSQSEENKLIDSPESGRKDTK-VLFAPDADDIPPINTARDFYREFCIELKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTS+CQYSFGAITQLFAGQVSTIALAAVS+ENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSICQYSFGAITQLFAGQVSTIALAAVSVENSVIAGFSFGIMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQL MMGVY+QRSWVILL+TAV+LTPIYIF+APLLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLGMMGVYLQRSWVILLTTAVVLTPIYIFSAPLLKLIGQTAEISEAAGVLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP +KFLQAQSKMMAMSVISAVALVFH  F+WL MLKLGWGL GGA+
Sbjct: 181 IWMIPQLYAYALNFPVSKFLQAQSKMMAMSVISAVALVFHTFFTWLFMLKLGWGLAGGAI 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW VI  AQIVYILSGSCG AW+GFSW+AF +LWGFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWVIDFAQIVYILSGSCGRAWSGFSWQAFHNLWGFVRLSLASAVMLCLEIWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSIDALSICTNI+GWTVMV FGINAAISVRVSNELGAAHPRTARFSLV
Sbjct: 301 LILFAGYLKNAEVSIDALSICTNILGWTVMVAFGINAAISVRVSNELGAAHPRTARFSLV 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAV SSFV+GLI  AIL++TK+DYP LFS+DSAVRQIVKNLTP+L FCI++NN+QPVLSG
Sbjct: 361 VAVASSFVVGLILTAILIITKDDYPYLFSNDSAVRQIVKNLTPMLGFCIVVNNIQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVN+GCYY+FG+P GLL+GF LHWGV GIWSGMI GT+IQT IL ++V
Sbjct: 421 VAVGAGWQAVVAYVNVGCYYLFGIPLGLLLGFALHWGVLGIWSGMIGGTIIQTFILVWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGG 492
           Y+TNWN+EASVAEDRIRKWGG
Sbjct: 481 YKTNWNEEASVAEDRIRKWGG 500

BLAST of Spg025927 vs. ExPASy TrEMBL
Match: A0A6J1GYV2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111458745 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.6e-230
Identity = 417/505 (82.57%), Postives = 456/505 (90.30%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADLSQPLL+P E+ K I SPESGR+ETKA  F PD  DIPPINGARDFYREF VEFKKL
Sbjct: 1   MADLSQPLLSPTEETKWIHSPESGRKETKA-KFVPDGGDIPPINGARDFYREFYVEFKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFT +CQYSFGAITQLFAGQVSTIALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTFICQYSFGAITQLFAGQVSTIALAAVSIENSVIAGFSFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDMMGVYMQRSWVILLSTA+ILTP+YIF+ PLLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTALILTPVYIFSVPLLKLIGQTAEISEAAGVLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP ++FLQAQSKMMAMSVISAVALV H  F+WLLM++LGWGL GGAV
Sbjct: 181 IWMIPQLYAYALNFPVSRFLQAQSKMMAMSVISAVALVLHTFFTWLLMVRLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW +IVVAQIVYILSGSCG AW+GFSWRAFQSL GFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWLIVVAQIVYILSGSCGRAWSGFSWRAFQSLSGFVRLSLASAVMLCLETWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSI+ALSICTN++GWT+MV FGINAAISVRVSNELGA HPRTARFSL+
Sbjct: 301 LILFAGYLKNAEVSINALSICTNVLGWTMMVAFGINAAISVRVSNELGAGHPRTARFSLI 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AA+L++TKN YP +FSSDSAVRQIVK+LT  L FCI++NNVQPVLSG
Sbjct: 361 VAVISSFVLGLIMAAVLIITKNSYPFIFSSDSAVRQIVKDLTLWLGFCIMVNNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA VAYVN+GCYY FGVP GLLMGFML WGVTGIW+GMI GT+IQTCIL ++V
Sbjct: 421 VAVGAGWQAAVAYVNVGCYYAFGVPLGLLMGFMLDWGVTGIWAGMIGGTIIQTCILIWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
            RTNW+ EASVAEDRIRKWGG S S
Sbjct: 481 QRTNWDTEASVAEDRIRKWGGPSIS 504

BLAST of Spg025927 vs. ExPASy TrEMBL
Match: A0A6J1DEY6 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111019813 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 7.6e-230
Identity = 408/505 (80.79%), Postives = 454/505 (89.90%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADL+Q LL+PR++ KR  SPES +RETKA  F+P ADDIPPIN  RDFYREFCVE +KL
Sbjct: 1   MADLTQQLLSPRDEEKRSHSPESRKRETKAATFSPGADDIPPINSVRDFYREFCVEVRKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTS+CQYSFGAITQL AGQVSTIALAAVS+ENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSICQYSFGAITQLLAGQVSTIALAAVSVENSVIAGFSLGIMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDM+G+YMQRSW+ILL+TAVI+TP Y+FAAPLLKL GQT  VSEAAG+MS
Sbjct: 121 LCGQAYGAGQLDMLGIYMQRSWIILLTTAVIMTPAYVFAAPLLKLTGQTEAVSEAAGVMS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYA+NFP +KFLQAQSKMMAMS I+AVALV H  FSWLLMLKLGWGL GGAV
Sbjct: 181 VWMIPQLYAYAVNFPISKFLQAQSKMMAMSAIAAVALVLHAFFSWLLMLKLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNA+W  +VVAQ+VYI  GSCG AW+GFSWRAFQSLWGFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNATWWFVVVAQLVYIFMGSCGRAWSGFSWRAFQSLWGFVRLSLASAVMLCLEIWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           LVLFAGYL++AEVSIDALSICTNI+GW VMV FGINAA+SVR+SNELGAAHPRTARFSLV
Sbjct: 301 LVLFAGYLEDAEVSIDALSICTNILGWMVMVAFGINAAVSVRISNELGAAHPRTARFSLV 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISS +LGL+ A+ILL+TKNDYPSLFS+DSAVRQIVK LTP+LCFCI+INNVQPVLSG
Sbjct: 361 VAVISSCLLGLMLASILLITKNDYPSLFSNDSAVRQIVKKLTPMLCFCIVINNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVNIGCYYVFGVP GLLMG+   +GV+GIWSGM+ GT+IQTCILT ++
Sbjct: 421 VAVGAGWQAVVAYVNIGCYYVFGVPLGLLMGYKFDFGVSGIWSGMMCGTIIQTCILTGMI 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           YRTNWNKEASVAEDRIRKWGG S S
Sbjct: 481 YRTNWNKEASVAEDRIRKWGGDSIS 505

BLAST of Spg025927 vs. ExPASy TrEMBL
Match: A0A6J1J9W8 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111483049 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.3e-229
Identity = 417/505 (82.57%), Postives = 458/505 (90.69%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           MADLSQPLL+P ++ K I SPESG +E KA  F PD  DIPPINGARDFYREF VEFKKL
Sbjct: 1   MADLSQPLLSPTDETKWIHSPESGTKEIKA-NFVPDGGDIPPINGARDFYREFYVEFKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFT +CQYSFGAITQLFAGQVSTIALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTFICQYSFGAITQLFAGQVSTIALAAVSIENSVIAGFSFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQL+MMGVYMQRSWVILLSTAVILTP+YIF+APLLKLIGQTAE+SEAAG++S
Sbjct: 121 LCGQAYGAGQLNMMGVYMQRSWVILLSTAVILTPVYIFSAPLLKLIGQTAEISEAAGVLS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP ++FLQAQSKMMAMSVISAVALV H  F+WLLM+KLGWGL GGAV
Sbjct: 181 IWMIPQLYAYALNFPVSRFLQAQSKMMAMSVISAVALVLHTFFTWLLMVKLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLNASW +IVVAQIVYILSGSCG AW+GFSWRAFQSL GFV+LSLASAVM+CLE WYFMA
Sbjct: 241 VLNASWWLIVVAQIVYILSGSCGRAWSGFSWRAFQSLSGFVRLSLASAVMLCLETWYFMA 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSI+ALSICTNI+GWT+MV FGINAAISVRVSNELGA HPRTARFSL+
Sbjct: 301 LILFAGYLKNAEVSINALSICTNILGWTMMVAFGINAAISVRVSNELGAGHPRTARFSLI 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AA+L++TKN+YP +FSSDSAVRQ+VK+LT  L FCI++NNVQPVLSG
Sbjct: 361 VAVISSFVLGLIMAAVLIITKNNYPFIFSSDSAVRQMVKDLTLWLGFCIVVNNVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA VAYVN+GCYYVFGVP GLLMGF+L WGVTGIWSGMI GT+IQTCIL ++V
Sbjct: 421 VAVGAGWQAAVAYVNVGCYYVFGVPLGLLMGFVLDWGVTGIWSGMIGGTIIQTCILIWMV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           +RTNW+ EASVAEDRIRKWGG S S
Sbjct: 481 HRTNWDTEASVAEDRIRKWGGSSIS 504

BLAST of Spg025927 vs. ExPASy TrEMBL
Match: A0A6J1HNV6 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111466382 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.7e-227
Identity = 417/505 (82.57%), Postives = 449/505 (88.91%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           M D SQPLL P ++ K I SPES R  T A +FAPDADDIPPIN ARDFYREF +E KKL
Sbjct: 1   MTDFSQPLLPPTDKTKWISSPESNREYT-APIFAPDADDIPPINNARDFYREFRIESKKL 60

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           WYLAAPAVFTSVCQYSFGAITQLFAGQVST+ALAAVSIENS          LGMGSALET
Sbjct: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTVALAAVSIENSVIAGFAFGVMLGMGSALET 120

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQAYGAGQLDMMGVY+QRS VIL+STAVILTP Y+FA PLL+ IGQTAEV+EAAG+MS
Sbjct: 121 LCGQAYGAGQLDMMGVYVQRSCVILVSTAVILTPFYVFATPLLRAIGQTAEVAEAAGVMS 180

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           +WMIPQLYAYALNFP +KFLQAQSKMMAMSVISAVALVFH  FSWLLMLKLGWGL GGAV
Sbjct: 181 VWMIPQLYAYALNFPISKFLQAQSKMMAMSVISAVALVFHAFFSWLLMLKLGWGLAGGAV 240

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           +LNASW +IVVAQIVYI+SG C   WTGFSWRAFQSLWGFVKLSLASAVM+CLE WYFM+
Sbjct: 241 ILNASWWLIVVAQIVYIMSGGCRETWTGFSWRAFQSLWGFVKLSLASAVMLCLEIWYFMS 300

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           L+LFAGYLKNAEVSI ALSIC NI+GW VMV+FGINAAISVRVSNELGAAHPRTARFSL+
Sbjct: 301 LILFAGYLKNAEVSIGALSICMNILGWMVMVSFGINAAISVRVSNELGAAHPRTARFSLI 360

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVISSFVLGLI AA++LVTKNDYP LFSSDSAVRQIVK LTPLLC CI+I+ VQPVLSG
Sbjct: 361 VAVISSFVLGLIMAAVVLVTKNDYPFLFSSDSAVRQIVKELTPLLCLCIVIDIVQPVLSG 420

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVNIG YYVFG+P GLLMGF+L+WGV GIW GMISG +IQT IL  IV
Sbjct: 421 VAVGAGWQALVAYVNIGSYYVFGLPLGLLMGFVLNWGVLGIWYGMISGIIIQTSILMIIV 480

Query: 481 YRTNWNKEASVAEDRIRKWGGHSDS 496
           YRTNWNKEASVAE+RIRKWGGHS S
Sbjct: 481 YRTNWNKEASVAENRIRKWGGHSVS 504

BLAST of Spg025927 vs. TAIR 10
Match: AT1G12950.1 (root hair specific 2 )

HSP 1 Score: 630.6 bits (1625), Expect = 1.1e-180
Identity = 321/501 (64.07%), Postives = 396/501 (79.04%), Query Frame = 0

Query: 1   MADLSQPLLAPREQNKRIDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKL 60
           +A   +  L P  Q   ++    G R +  V F+  A DIPPI+G  DF REF +E +KL
Sbjct: 12  LASTEEEELDPATQKALMEYLGVGSRASSLVSFSSTAVDIPPISGVGDFVREFRIESRKL 71

Query: 61  WYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVSIENS----------LGMGSALET 120
           W LA PA+FT++ QYS GA+TQ+FAG +ST+ALAAVSIENS          LGMGSALET
Sbjct: 72  WKLAGPAIFTTMSQYSLGAVTQVFAGHISTLALAAVSIENSVIAGFSFGIMLGMGSALET 131

Query: 121 LCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMS 180
           LCGQA+GAG++ M+GVY+QRSWVIL  TA+ L+ IYIFAAP+L  IGQTA +S  AG+ S
Sbjct: 132 LCGQAFGAGKVSMLGVYLQRSWVILSVTALFLSLIYIFAAPILTFIGQTAAISAMAGIFS 191

Query: 181 LWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAV 240
           ++MIPQ++AYA+NFPTAKFLQ+QSK+M M+ IS V LV H  F+WL+M +L WGL G A+
Sbjct: 192 IYMIPQIFAYAINFPTAKFLQSQSKIMVMAGISGVVLVIHSFFTWLVMSRLHWGLPGLAL 251

Query: 241 VLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMA 300
           VLN SW VIVVAQ+VYI + +CG AW+GF+W AF +LWGFVKLSLASA M+CLE WYFMA
Sbjct: 252 VLNTSWWVIVVAQLVYIFNCTCGEAWSGFTWEAFHNLWGFVKLSLASAAMLCLEIWYFMA 311

Query: 301 LVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLV 360
           LVLFAGYLKNAEVS+ ALSIC NI+GW  MV FG NAA+SVRVSNELGA+HPRTA+FSLV
Sbjct: 312 LVLFAGYLKNAEVSVAALSICMNILGWAAMVAFGTNAAVSVRVSNELGASHPRTAKFSLV 371

Query: 361 VAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSG 420
           VAVI S  +G+  AA LL  +N+YP LF  D  VR +V+ LTP+L FCI+INNVQPVLSG
Sbjct: 372 VAVILSTAIGMFIAAGLLFFRNEYPVLFVEDEEVRNVVRELTPMLAFCIVINNVQPVLSG 431

Query: 421 VAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIV 480
           VAVGAGWQA+VAYVNI CYY+FGVPFGLL+GF L +GV GIW GM++GT +Q+ +LT+++
Sbjct: 432 VAVGAGWQAVVAYVNIACYYLFGVPFGLLLGFKLEYGVMGIWWGMVTGTFVQSIVLTWMI 491

Query: 481 YRTNWNKEASVAEDRIRKWGG 492
            +TNW KEAS+AE+RI++WGG
Sbjct: 492 CKTNWEKEASMAEERIKEWGG 512

BLAST of Spg025927 vs. TAIR 10
Match: AT3G26590.1 (MATE efflux family protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.4e-180
Identity = 315/464 (67.89%), Postives = 387/464 (83.41%), Query Frame = 0

Query: 38  DDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQLFAGQVSTIALAAVS 97
           DDIPPI     F REF VE KKLWYLA PA+FTSV QYS GAITQ+FAG +STIALAAVS
Sbjct: 27  DDIPPITTVGGFVREFNVETKKLWYLAGPAIFTSVNQYSLGAITQVFAGHISTIALAAVS 86

Query: 98  IENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSWVILLSTAVILTPIYI 157
           +ENS          LGMGSALETLCGQA+GAG+L M+GVY+QRSWVIL  TA+IL+ +YI
Sbjct: 87  VENSVVAGFSFGIMLGMGSALETLCGQAFGAGKLSMLGVYLQRSWVILNVTALILSLLYI 146

Query: 158 FAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQAQSKMMAMSVISAVAL 217
           FAAP+L  IGQTA +S AAG+ S++MIPQ++AYA+NFPTAKFLQ+QSK+M M+VISAVAL
Sbjct: 147 FAAPILASIGQTAAISSAAGIFSIYMIPQIFAYAINFPTAKFLQSQSKIMVMAVISAVAL 206

Query: 218 VFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSCGPAWTGFSWRAFQSL 277
           V HV  +W +++KL WG+ G AVVLNASWC I +AQ+VYI SG+CG AW+GFSW AF +L
Sbjct: 207 VIHVPLTWFVIVKLQWGMPGLAVVLNASWCFIDMAQLVYIFSGTCGEAWSGFSWEAFHNL 266

Query: 278 WGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICTNIVGWTVMVTFGINA 337
           W FV+LSLASAVM+CLE WYFMA++LFAGYLKNAE+S+ ALSIC NI+GWT M+  G+N 
Sbjct: 267 WSFVRLSLASAVMLCLEVWYFMAIILFAGYLKNAEISVAALSICMNILGWTAMIAIGMNT 326

Query: 338 AISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKNDYPSLFSSDSAVRQI 397
           A+SVRVSNELGA HPRTA+FSL+VAVI+S ++G I + ILL+ ++ YPSLF  D  V  +
Sbjct: 327 AVSVRVSNELGANHPRTAKFSLLVAVITSTLIGFIVSMILLIFRDQYPSLFVKDEKVIIL 386

Query: 398 VKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVFGVPFGLLMGFMLHWG 457
           VK LTP+L   I+INNVQPVLSGVAVGAGWQA+VAYVNI CYYVFG+PFGLL+G+ L++G
Sbjct: 387 VKELTPILALSIVINNVQPVLSGVAVGAGWQAVVAYVNIACYYVFGIPFGLLLGYKLNYG 446

Query: 458 VTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           V GIW GM++GT++QT +LT+++ +TNW+ EAS+AEDRIR+WGG
Sbjct: 447 VMGIWCGMLTGTVVQTIVLTWMICKTNWDTEASMAEDRIREWGG 490

BLAST of Spg025927 vs. TAIR 10
Match: AT5G38030.1 (MATE efflux family protein )

HSP 1 Score: 611.3 bits (1575), Expect = 6.9e-175
Identity = 311/479 (64.93%), Postives = 385/479 (80.38%), Query Frame = 0

Query: 23  SGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQ 82
           S   E  A+ F+   +DIPPI     F +EF VE KKLWYLA PA+F S+ QYS GA TQ
Sbjct: 13  SAAEEPPALPFS-SVEDIPPITTVGGFVKEFNVEVKKLWYLAGPAIFMSITQYSLGAATQ 72

Query: 83  LFAGQVSTIALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSW 142
           +FAG +STIALAAVS+ENS          LGMGSALETLCGQA+GAG+L M+GVY+QRSW
Sbjct: 73  VFAGHISTIALAAVSVENSVIAGFSFGVMLGMGSALETLCGQAFGAGKLSMLGVYLQRSW 132

Query: 143 VILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQA 202
           VIL  TAVIL+ +YIFAAP+L  IGQT  +S A G+ S++MIPQ++AYA+N+PTAKFLQ+
Sbjct: 133 VILNVTAVILSLLYIFAAPILAFIGQTPAISSATGIFSIYMIPQIFAYAVNYPTAKFLQS 192

Query: 203 QSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSC 262
           QSK+M M+ ISAVALV HVL +W ++  L WG  G AVVLNASW  IVVAQ+VYI SG+C
Sbjct: 193 QSKIMVMAAISAVALVLHVLLTWFVIEGLQWGTAGLAVVLNASWWFIVVAQLVYIFSGTC 252

Query: 263 GPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICT 322
           G AW+GFSW AF +LW FV+LSLASAVM+CLE WY MA++LFAGYLKNAE+S+ ALSIC 
Sbjct: 253 GEAWSGFSWEAFHNLWSFVRLSLASAVMLCLEVWYLMAVILFAGYLKNAEISVAALSICM 312

Query: 323 NIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKN 382
           NI+GWT M+  G+NAA+SVRVSNELGA HPRTA+FSL+VAVI+S V+GL  +  LL+ ++
Sbjct: 313 NILGWTAMIAIGMNAAVSVRVSNELGAKHPRTAKFSLLVAVITSTVIGLAISIALLIFRD 372

Query: 383 DYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVF 442
            YPSLF  D  V  +VK+LTP+L   I+INNVQPVLSGVAVGAGWQA+VAYVNI CYYVF
Sbjct: 373 KYPSLFVGDEEVIIVVKDLTPILAVSIVINNVQPVLSGVAVGAGWQAVVAYVNIVCYYVF 432

Query: 443 GVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           G+PFGLL+G+ L++GV GIW GM++GT++QT +LT+++ RTNW+ EA++AE RIR+WGG
Sbjct: 433 GIPFGLLLGYKLNFGVMGIWCGMLTGTVVQTIVLTWMICRTNWDTEAAMAEGRIREWGG 490

BLAST of Spg025927 vs. TAIR 10
Match: AT1G23300.1 (MATE efflux family protein )

HSP 1 Score: 609.0 bits (1569), Expect = 3.4e-174
Identity = 308/487 (63.24%), Postives = 383/487 (78.64%), Query Frame = 0

Query: 18  IDSPESGRRETKAVVFAPDADDIPPINGARDFYREFCVEFKKLWYLAAPAVFTSVCQYSF 77
           I S +  R  TK+        D+PPI+G RDF R+F  E KKLW+LA PA+FTS CQYS 
Sbjct: 12  ISSEQEHRAHTKS------DTDMPPISGGRDFIRQFAAESKKLWWLAGPAIFTSFCQYSL 71

Query: 78  GAITQLFAGQVSTIALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVY 137
           GA+TQ+ AG V+T+ALAAVSI+NS          LGMGSAL TLCGQAYGAGQL+MMG+Y
Sbjct: 72  GAVTQILAGHVNTLALAAVSIQNSVISGFSVGIMLGMGSALATLCGQAYGAGQLEMMGIY 131

Query: 138 MQRSWVILLSTAVILTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTA 197
           +QRSW+IL S A++L   Y+FA PLL L+GQ+ E+S+AAG  SLWMIPQL+AYA+NF TA
Sbjct: 132 LQRSWIILNSCALLLCLFYVFATPLLSLLGQSPEISKAAGKFSLWMIPQLFAYAVNFATA 191

Query: 198 KFLQAQSKMMAMSVISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYI 257
           KFLQAQSK++AM+VI+A  L+ H L SWLLMLKL WG+ GGAVVLN SW +I V QIVYI
Sbjct: 192 KFLQAQSKVIAMAVIAATVLLQHTLLSWLLMLKLRWGMAGGAVVLNMSWWLIDVTQIVYI 251

Query: 258 LSGSCGPAWTGFSWRAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDA 317
             GS G AW+G SW AF++L GF +LSLASAVM+CLE WYFMAL+LFAGYLKN +VS+ A
Sbjct: 252 CGGSSGRAWSGLSWMAFKNLRGFARLSLASAVMVCLEVWYFMALILFAGYLKNPQVSVAA 311

Query: 318 LSICTNIVGWTVMVTFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAIL 377
           LSIC NI+GW +MV FG NAA+SVR SNELGA HPR A+F L+VA+I+S  +G++ +  L
Sbjct: 312 LSICMNILGWPIMVAFGFNAAVSVRESNELGAEHPRRAKFLLIVAMITSVSIGIVISVTL 371

Query: 378 LVTKNDYPSLFSSDSAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIG 437
           +V ++ YP++FS D  VR +VK LTPLL   I+INN+QPVLSGVAVGAGWQ IVAYVNIG
Sbjct: 372 IVLRDKYPAMFSDDEEVRVLVKQLTPLLALTIVINNIQPVLSGVAVGAGWQGIVAYVNIG 431

Query: 438 CYYVFGVPFGLLMGFMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIR 495
           CYY+ G+P GL++G+ +  GV GIW+GM++GT++QT +L FI+YRTNW KEAS+AE RI+
Sbjct: 432 CYYLCGIPIGLVLGYKMELGVKGIWTGMLTGTVVQTSVLLFIIYRTNWKKEASLAEARIK 491

BLAST of Spg025927 vs. TAIR 10
Match: AT1G47530.1 (MATE efflux family protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.1e-143
Identity = 259/470 (55.11%), Postives = 338/470 (71.91%), Query Frame = 0

Query: 36  DADDIPPINGARD----FYREFCVEFKKLWYLAAPAVFTSVCQYSFGAITQLFAGQVSTI 95
           D  + P + G +     + +EF  E K+LW LA PA+FT++ QYS GA+TQ F+G++  +
Sbjct: 11  DPREPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGALTQTFSGRLGEL 70

Query: 96  ALAAVSIENS----------LGMGSALETLCGQAYGAGQLDMMGVYMQRSWVILLSTAVI 155
            LAAVS+ENS          LGMGSALETLCGQAYGAGQ+ MMG+YMQRSWVIL +TA+ 
Sbjct: 71  ELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQRSWVILFTTALF 130

Query: 156 LTPIYIFAAPLLKLIGQTAEVSEAAGLMSLWMIPQLYAYALNFPTAKFLQAQSKMMAMSV 215
           L P+YI+A P+L   G+   +S+AAG  +LWMIPQL+AYA NFP  KFLQ+Q K++ M+ 
Sbjct: 131 LLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFLQSQRKVLVMAW 190

Query: 216 ISAVALVFHVLFSWLLMLKLGWGLGGGAVVLNASWCVIVVAQIVYILSGSCGPAWTGFSW 275
           IS V LV H +FSWL +L   WGL G A+ LN SW +IV+ Q++YIL      AWTGFS 
Sbjct: 191 ISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILITKSDGAWTGFSM 250

Query: 276 RAFQSLWGFVKLSLASAVMMCLEAWYFMALVLFAGYLKNAEVSIDALSICTNIVGWTVMV 335
            AF+ L+GFVKLSLASA+M+CLE WY M LV+  G L N  + +DA+SIC NI GWT M+
Sbjct: 251 LAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISICMNIEGWTAMI 310

Query: 336 TFGINAAISVRVSNELGAAHPRTARFSLVVAVISSFVLGLIFAAILLVTKNDYPSLFSSD 395
           + G NAAISVRVSNELGA +   A+FS++V  I+S ++G++   ++L TK+ +P LF+S 
Sbjct: 311 SIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLATKDSFPYLFTSS 370

Query: 396 SAVRQIVKNLTPLLCFCILINNVQPVLSGVAVGAGWQAIVAYVNIGCYYVFGVPFGLLMG 455
            AV      +  LL F +L+N++QPVLSGVAVGAGWQA+VAYVNI CYY+ G+P GL++G
Sbjct: 371 EAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYYIIGLPAGLVLG 430

Query: 456 FMLHWGVTGIWSGMISGTMIQTCILTFIVYRTNWNKEASVAEDRIRKWGG 492
           F L  GV GIW GM++G  +QT IL  I+Y TNWNKEA  AE R+++WGG
Sbjct: 431 FTLDLGVQGIWGGMVAGICLQTLILIGIIYFTNWNKEAEQAESRVQRWGG 480