Spg025899 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025899 vs. NCBI nr
Match: XP_038892208.1 (protein argonaute 7 [Benincasa hispida])

HSP 1 Score: 1864.7 bits (4829), Expect = 0.0e+00
Identity = 908/951 (95.48%), Postives = 931/951 (97.90%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLPPPIPLQLALAP+LPPN+T RPK HLQKPSCRLNNPPLAA SDTHVPI+KISPA
Sbjct: 80   PLPLPLPPPIPLQLALAPSLPPNKTIRPKPHLQKPSCRLNNPPLAANSDTHVPIIKISPA 139

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            PKGLRLKSSLPLKGEN K+SESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 140  PKGLRLKSSLPLKGENAKLSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 199

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLV+DNSDLLSGASPAYDGRKNLYS IEFVRDRLEFYVS
Sbjct: 200  YHYNVEISPNPSKEVARMIKQKLVKDNSDLLSGASPAYDGRKNLYSSIEFVRDRLEFYVS 259

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MKV+EGEI+HKQ FKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA
Sbjct: 260  LPIPSSRMKVTEGEIEHKQAFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 319

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C P+GRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMN+DCSV
Sbjct: 320  LDVVLREGPNEKCIPLGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNIDCSV 379

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLR+LSQRK+RGLT EER+EVEKAL HIRVFVCHRESVQRYR
Sbjct: 380  TAFHESIGVIPYLQKRLDFLRELSQRKSRGLTIEERKEVEKALMHIRVFVCHRESVQRYR 439

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 440  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 499

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 500  ICEGQKFLGKLTDEQTTRMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 559

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFE TRIERW+LISFGG PD
Sbjct: 560  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEATRIERWALISFGGVPD 619

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QRLNIPRFINQLSRRC+QLGI+LNSKT+VPPQFEPTQVLNSVSLLESKLKKLHGAASNNL
Sbjct: 620  QRLNIPRFINQLSRRCDQLGIFLNSKTIVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 679

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAET IGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 680  QLLICVMERKHKGYADLKRIAETGIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 739

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDD SPS+AAVVGSMNWPAANKYVSRM
Sbjct: 740  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDVSPSIAAVVGSMNWPAANKYVSRM 799

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 800  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 859

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 860  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 919

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 920  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 979

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKT PLPKLSENVRKLMFYC
Sbjct: 980  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTTPLPKLSENVRKLMFYC 1030

BLAST of Spg025899 vs. NCBI nr
Match: KAA0034448.1 (protein argonaute 7 [Cucumis melo var. makuwa] >TYK17686.1 protein argonaute 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1852.4 bits (4797), Expect = 0.0e+00
Identity = 905/951 (95.16%), Postives = 928/951 (97.58%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MKVSEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKVSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EER+EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEERKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWGLISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRMDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST HTRG+STVSRAAPPKTMPLPKL+ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTHTRGISTVSRAAPPKTMPLPKLNENVRKLMFYC 1019

BLAST of Spg025899 vs. NCBI nr
Match: XP_008446401.1 (PREDICTED: protein argonaute 7 [Cucumis melo])

HSP 1 Score: 1850.1 bits (4791), Expect = 0.0e+00
Identity = 904/951 (95.06%), Postives = 927/951 (97.48%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MKVSEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKVSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EER+EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEERKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RM KMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMQKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWGLISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRMDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST HTRG+STVSRAAPPKTMPLPKL+ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTHTRGISTVSRAAPPKTMPLPKLNENVRKLMFYC 1019

BLAST of Spg025899 vs. NCBI nr
Match: XP_022151236.1 (protein argonaute 7 [Momordica charantia])

HSP 1 Score: 1849.7 bits (4790), Expect = 0.0e+00
Identity = 906/951 (95.27%), Postives = 928/951 (97.58%), Query Frame = 0

Query: 2    LPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPAP 61
            LPLPLPPPIPLQLALAP LPPN TT  KTH QKPSCRLN PPLAA SDTH+PI+KISPAP
Sbjct: 73   LPLPLPPPIPLQLALAP-LPPNTTTTTKTHFQKPSCRLNTPPLAAPSDTHLPIIKISPAP 132

Query: 62   KGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRIY 121
            KGLRLKS+LPLKGENGKVSESKPE LVAARRPDSGGVEGP IPLLANHFLVQFDPSQRI 
Sbjct: 133  KGLRLKSNLPLKGENGKVSESKPEALVAARRPDSGGVEGPFIPLLANHFLVQFDPSQRIC 192

Query: 122  HYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSL 181
            HYNVEISPNPSKE+ARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFY+SL
Sbjct: 193  HYNVEISPNPSKEIARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYISL 252

Query: 182  PIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHAL 241
            PIPS+KMKV+ GEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDW PIPQDYLHAL
Sbjct: 253  PIPSNKMKVTGGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWTPIPQDYLHAL 312

Query: 242  DVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT 301
            DVVLRE PNE CFPVGRSLYSSSMWG K+IGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT
Sbjct: 313  DVVLRETPNEKCFPVGRSLYSSSMWGGKDIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT 372

Query: 302  AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV 361
            AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV
Sbjct: 373  AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV 432

Query: 362  YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCMI 421
            YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNY+IQ+RNLPCLQISRSKPCYLPMELCMI
Sbjct: 433  YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYEIQFRNLPCLQISRSKPCYLPMELCMI 492

Query: 422  CEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREM 481
            CEGQ+FLGKLSDEQTARMLKMGCQRP+ERKANIDGVMQGPVGPTSG QGREF+LQVSREM
Sbjct: 493  CEGQRFLGKLSDEQTARMLKMGCQRPRERKANIDGVMQGPVGPTSGNQGREFSLQVSREM 552

Query: 482  TKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQ 541
            TKL+GRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW+LISFGGS DQ
Sbjct: 553  TKLTGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWALISFGGSLDQ 612

Query: 542  RLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNLQ 601
            RLNIPRFINQLSRRCEQLGI+LNSK VVPPQFEPTQVLNSVS+LESKLKKLHGAASN L+
Sbjct: 613  RLNIPRFINQLSRRCEQLGIFLNSKPVVPPQFEPTQVLNSVSILESKLKKLHGAASNKLE 672

Query: 602  LLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV 661
            LLICVMERKHKGYADLKRIAETSIG+VSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV
Sbjct: 673  LLICVMERKHKGYADLKRIAETSIGIVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV 732

Query: 662  ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMR 721
            ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRMR
Sbjct: 733  ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRMR 792

Query: 722  SQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKTA 781
            SQTHRQEIIVDLGAMVEELL+EFYQEVNELPSRIIFFRDGVSETQF+KVLQEELQAIKTA
Sbjct: 793  SQTHRQEIIVDLGAMVEELLDEFYQEVNELPSRIIFFRDGVSETQFFKVLQEELQAIKTA 852

Query: 782  CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEFD 841
            CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN NEVLDENIPPGTVVDTVITHPKEFD
Sbjct: 853  CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQNEVLDENIPPGTVVDTVITHPKEFD 912

Query: 842  FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH 901
            FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH
Sbjct: 913  FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH 972

Query: 902  LAAYRGRLYLERSD-STAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            LAAYRGRLYLERSD STAHTRGVS VSRAAPPKT PLPKLSENVRKLMFYC
Sbjct: 973  LAAYRGRLYLERSDYSTAHTRGVSAVSRAAPPKTTPLPKLSENVRKLMFYC 1022

BLAST of Spg025899 vs. NCBI nr
Match: XP_004135160.1 (protein argonaute 7 [Cucumis sativus])

HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 900/951 (94.64%), Postives = 925/951 (97.27%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MK SEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKFSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EE++EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEEKKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW+LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWALISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRIDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRF NYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFLNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST +TRG+STVSRAAPPKT PLPKL ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTYTRGISTVSRAAPPKTTPLPKLKENVRKLMFYC 1019

BLAST of Spg025899 vs. ExPASy Swiss-Prot
Match: Q9C793 (Protein argonaute 7 OS=Arabidopsis thaliana OX=3702 GN=AGO7 PE=2 SV=1)

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 636/955 (66.60%), Postives = 756/955 (79.16%), Query Frame = 0

Query: 7   PPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTH--VPILKISPAPKGL 66
           PPP P  L L+P LPP                L  PP  + +  H  +P+ ++    +  
Sbjct: 80  PPPPPHLLPLSPPLPP---------------LLPLPPPHSMTRFHKSLPVSQVVERKQQH 139

Query: 67  RLKSSLPLKGENGKVSES---KPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRIY 126
           + K  + +   N KVS S   +   LV A+RPD GG +G VI LLANHFLV+FD SQRIY
Sbjct: 140 QQKKKIQV--SNNKVSGSIAIEEAALVVAKRPDFGGQDGSVIYLLANHFLVKFDSSQRIY 199

Query: 127 HYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSL 186
           HYNVEISP PSKE+ARMIKQKLVE + +  SG  PA+DGR+N+YSP+EF  DRLEF+V+L
Sbjct: 200 HYNVEISPQPSKEIARMIKQKLVETDRNSFSGVVPAFDGRQNIYSPVEFQGDRLEFFVNL 259

Query: 187 PIPSSKMKVSEGEIKHKQE----FKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDY 246
           PIPS K  ++ G+++ KQ      KLFR+++KLVSKFDG E      KEG+DW P+P +Y
Sbjct: 260 PIPSCKAVMNYGDLREKQPQKKIEKLFRVNMKLVSKFDGKEQR----KEGEDWAPLPPEY 319

Query: 247 LHALDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVD 306
           +HALDV+LRE P E C  +GRS YSSSM GSKEIGGG VGLRGFFQSLR TQQGLA+N+D
Sbjct: 320 IHALDVILRENPMEKCTSIGRSFYSSSMGGSKEIGGGAVGLRGFFQSLRHTQQGLALNMD 379

Query: 307 CSVTAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQ 366
            S+TAFHESIGVI YLQKRL+FL DL + K R L+ EE+REVEKALK+IRVFVCHRE+VQ
Sbjct: 380 LSITAFHESIGVIAYLQKRLEFLTDLPRNKGRELSLEEKREVEKALKNIRVFVCHRETVQ 439

Query: 367 RYRVYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPME 426
           RYRVYGLT+E TE++WF DR GK LRL+ YFKDHY Y+IQ++NLPCLQISR++PCYLPME
Sbjct: 440 RYRVYGLTEEITENIWFPDREGKYLRLMSYFKDHYGYEIQFKNLPCLQISRARPCYLPME 499

Query: 427 LCMICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQV 486
           LCMICEGQKFLGKLSD+Q A+++KMGCQ+P ERKA ID VM G VGP+SG Q REFNL+V
Sbjct: 500 LCMICEGQKFLGKLSDDQAAKIMKMGCQKPNERKAIIDKVMTGSVGPSSGNQTREFNLEV 559

Query: 487 SREMTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGG 546
           SREMT L GR+LQPPKLKL              DR  NL +  VF+GTRIERW+L+S GG
Sbjct: 560 SREMTLLKGRILQPPKLKL--------------DRPRNLKESKVFKGTRIERWALMSIGG 619

Query: 547 SPDQRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAAS 606
           S DQ+  IP+FIN+L+++CE LG++L+  T+    FEP+ +LN++SLLESKLK++  AAS
Sbjct: 620 SSDQKSTIPKFINELTQKCEHLGVFLSKNTLSSTFFEPSHILNNISLLESKLKEIQRAAS 679

Query: 607 NNLQLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVG 666
           NNLQL+ICVME+KHKGY DLKRI+ET IGVV+QCCLYPN+ KLSSQF++NLALKINAK+G
Sbjct: 680 NNLQLIICVMEKKHKGYGDLKRISETRIGVVTQCCLYPNITKLSSQFVSNLALKINAKIG 739

Query: 667 GCTVALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYV 726
           G    LYNS+PS +PRLLR D+PVIFMGADVTHPHP DD SPSVAAVVGS+NWP AN+YV
Sbjct: 740 GSMTELYNSIPSHIPRLLRPDEPVIFMGADVTHPHPFDDCSPSVAAVVGSINWPEANRYV 799

Query: 727 SRMRSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQA 786
           SRMRSQTHRQEII DL  MV+ELL++FY+ V +LP+RIIFFRDGVSETQF KVLQEELQ+
Sbjct: 800 SRMRSQTHRQEIIQDLDLMVKELLDDFYKAVKKLPNRIIFFRDGVSETQFKKVLQEELQS 859

Query: 787 IKTACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHP 846
           IKTACS+F +Y P ITFAVVQKRHHTRL  F+ DP         ENIPPGTVVDTVITHP
Sbjct: 860 IKTACSKFQDYNPSITFAVVQKRHHTRL--FRCDPD-------HENIPPGTVVDTVITHP 919

Query: 847 KEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPA 906
           KEFDFYLCSH GVKGTSRPTHYH+L DEN+FTSDELQ+LVYNLCYT+ RCTKP+S+VPPA
Sbjct: 920 KEFDFYLCSHLGVKGTSRPTHYHILWDENEFTSDELQRLVYNLCYTFVRCTKPISIVPPA 979

Query: 907 YYAHLAAYRGRLYLER-SDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
           YYAHLAAYRGRLY+ER S+S   +   S+VSR  PPKT+PLPKLS+NV+ LMFYC
Sbjct: 980 YYAHLAAYRGRLYIERSSESNGGSMNPSSVSRVGPPKTIPLPKLSDNVKNLMFYC 990

BLAST of Spg025899 vs. ExPASy Swiss-Prot
Match: Q75HC2 (Protein argonaute 7 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO7 PE=2 SV=2)

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 621/979 (63.43%), Postives = 748/979 (76.40%), Query Frame = 0

Query: 2    LPLPLPPPIPLQLALAPALPPNRTTRPK----THLQKPSCRLNNPPLAA--------TSD 61
            LPLPLPPP+ L L   P  PP     PK    T   KP    +  P  +           
Sbjct: 83   LPLPLPPPVLLYLQ-PPPPPPLLPLLPKVAAATFYGKPPKAADAAPRGSMWKHRPSKKPP 142

Query: 62   THVPILKISPAPK-GLRLKSSLPLKGENGKVSESK-------------PEVLVAARRPDS 121
             H     + P P+ G  L+  +    E  K SE +             P     ARRPD 
Sbjct: 143  PHAITAALLPLPRDGKALQEKIFFANER-KTSEKEVNHVDTHEKFTVAPLDNAIARRPDM 202

Query: 122  GGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGAS 181
            GGVEG  IPL ANHFLVQFDP Q+I+HYNV+ISP PSKE ARMIK+KLVE+N  +LSG+ 
Sbjct: 203  GGVEGAEIPLSANHFLVQFDPGQKIFHYNVDISPRPSKETARMIKKKLVEENPSVLSGSQ 262

Query: 182  PAYDGRKNLYSPIEFVRDRLEFYVSLPIP---SSKMKVSEGEIKHKQEFKLFRISIKLVS 241
            PA+DGRKNLYSP+ F  DR+EF+VSLP+     S +K   G +  KQ+ K F+++++LVS
Sbjct: 263  PAFDGRKNLYSPVRFQEDRVEFFVSLPVALARCSVVKEDTGHMLDKQKLKTFKVNVRLVS 322

Query: 242  KFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENCFPVGRSLYSSSMWGSKEIG 301
            K  G +LN+YL  E  D IP+PQDYLHALDVVLREG  E+   VGRSLY+ SM  +++IG
Sbjct: 323  KLCGEDLNKYL-NEDKDGIPLPQDYLHALDVVLREGAMESSILVGRSLYARSMGEARDIG 382

Query: 302  GGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT 361
            GG VGLRGFFQ LRPT+QGLA+NVD S++AFHES G+I YLQKR DFL+DL Q+KTR L 
Sbjct: 383  GGAVGLRGFFQRLRPTKQGLALNVDLSLSAFHESTGIISYLQKRCDFLKDLPQKKTRALA 442

Query: 362  CEERREVEKALKHIRVFVCHRESVQRYRVYGLTDEPTESLWFADRNGKNLRLVGYFKDHY 421
             EE REVEKALK+IRVFVCHRE+ QRY V+ LT E TE+L F DR+GK+L +V YFK+HY
Sbjct: 443  EEEHREVEKALKNIRVFVCHRETNQRYHVHSLTKETTENLKFRDRSGKDLMVVDYFKEHY 502

Query: 422  NYDIQYRNLPCLQISRSKPCYLPMELCMICEGQKFLGKLSDEQTARMLKMGCQRPKERKA 481
            N+DIQ+RNLPCLQI RSKPCY+PMELC++CEGQKFLGKLSDEQT+++LKMGC+RP ERK 
Sbjct: 503  NHDIQFRNLPCLQIGRSKPCYVPMELCVVCEGQKFLGKLSDEQTSKILKMGCERPSERKG 562

Query: 482  NIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDR 541
             I GV++G     S     +F+LQVS+ MTKLSGRVL PPKLKLG  G+I+D+ P R DR
Sbjct: 563  IIKGVVKGAFHARSDTYADQFSLQVSKHMTKLSGRVLLPPKLKLGSSGRIKDITPDRFDR 622

Query: 542  QWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQ 601
            QW+ +D HV EG++I+ W+LISFGG+P+Q   I +F+NQLS RCEQLGI LN KT++ P 
Sbjct: 623  QWSFLDSHVAEGSKIKSWALISFGGTPEQHFCITKFVNQLSNRCEQLGILLNKKTIISPI 682

Query: 602  FEPTQVLNSVSLLESKLKKLHGAASNNLQLLICVMERKHKGYADLKRIAETSIGVVSQCC 661
            FE  Q+LN+V +LE KLKK+  AAS NLQLLICVMER+H+GYADLKRIAETSIGVV+QCC
Sbjct: 683  FERIQLLNNVGILEGKLKKIQEAASGNLQLLICVMERRHQGYADLKRIAETSIGVVTQCC 742

Query: 662  LYPNLAKLSSQFLANLALKINAKVGGCTVALYNSLPSQVPRLLRTDKPVIFMGADVTHPH 721
            LY NL+KL+SQFL NLALKINAK+GGC +ALY+S P Q+PR+  +++PV+FMGADVTHPH
Sbjct: 743  LYSNLSKLTSQFLTNLALKINAKLGGCNIALYSSFPCQIPRIFLSEEPVMFMGADVTHPH 802

Query: 722  PLDDFSPSVAAVVGSMNWPAANKYVSRMRSQTHRQEIIVDLGAMVEELLEEFYQEVNELP 781
            PLDD SPSV AVV SMNWP+ANKY+SRMRSQTHR+EII  L  M  ELLEEF +EV +LP
Sbjct: 803  PLDDSSPSVVAVVASMNWPSANKYISRMRSQTHRKEIIEQLDVMAGELLEEFLKEVGKLP 862

Query: 782  SRIIFFRDGVSETQFYKVLQEELQAIKTACSRFHNYKPPITFAVVQKRHHTRLFPFKVDP 841
            SRIIFFRDGVSETQFYKVL+EE+ A++T CSR+  YKP ITF VVQKRHHTRLF  + + 
Sbjct: 863  SRIIFFRDGVSETQFYKVLKEEMHAVRTTCSRYPGYKPLITFIVVQKRHHTRLFHRERNG 922

Query: 842  SSNHNEVLDENIPPGTVVDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDE 901
            SS+H    D+NIPPGTVVDTVITHP+EFDFYLCSHWG KGTSRPTHYHVL DEN F SDE
Sbjct: 923  SSSHYS--DQNIPPGTVVDTVITHPREFDFYLCSHWGTKGTSRPTHYHVLWDENNFRSDE 982

Query: 902  LQKLVYNLCYTYNRCTKPVSLVPPAYYAHLAAYRGRLYLERSDSTAHTRGVSTVSRAAPP 952
            +Q+L++NLCYT+ RCT+PVSLVPPAYYAHLAAYRGRLYLERSD+T +        R +P 
Sbjct: 983  VQQLIHNLCYTFARCTRPVSLVPPAYYAHLAAYRGRLYLERSDTTMY--------RVSPL 1042

BLAST of Spg025899 vs. ExPASy Swiss-Prot
Match: Q7XSA2 (Protein argonaute 1B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1B PE=2 SV=3)

HSP 1 Score: 642.5 bits (1656), Expect = 7.5e-183
Identity = 387/955 (40.52%), Postives = 551/955 (57.70%), Query Frame = 0

Query: 57   ISPAPKGLRLKSSLPLKGENGKV----------SESKPEVLVAARRPDSGGVEGPVIP-- 116
            +SP P G    S    +  +G+V           +S     +    P S  V  P+ P  
Sbjct: 191  VSPTPSGAGSSSQPAAEVSSGQVQQQFQQLATRDQSSTSQAIQIAPPSSKSVRFPLRPGK 250

Query: 117  --------LLANHFLVQFDPSQRIYHYNVEISPN-PSKEVARMIKQKLVE-DNSDLLSGA 176
                    + ANHF  +  P + ++ Y+V I+P   S+ V R +  +LV       L G 
Sbjct: 251  GTYGDRCIVKANHFFAEL-PDKDLHQYDVSITPEVTSRGVNRAVMFELVTLYRYSHLGGR 310

Query: 177  SPAYDGRKNLYS--PIEFVRDRLEFYVSLPIPSSKMKVSEGEIKHKQEFKLFRISIKLVS 236
             PAYDGRK+LY+  P+ F     E    + +   +  +  G+   ++E +LFR+ IK  +
Sbjct: 311  LPAYDGRKSLYTAGPLPFASRTFE----ITLQDEEDSLGGGQGTQRRE-RLFRVVIKFAA 370

Query: 237  KFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENCFPVGRSLYSSSMWGSKEIG 296
            + D   L  +L     D    PQ+ L  LD+VLRE P     PVGRS YS ++   +++G
Sbjct: 371  RADLHHLAMFLAGRQAD---APQEALQVLDIVLRELPTTRYSPVGRSFYSPNLGRRQQLG 430

Query: 297  GGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT 356
             G+   RGF+QS+RPTQ GL++N+D S TAF E + VI ++ + L+  RD+S    R L+
Sbjct: 431  EGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLN--RDIS---VRPLS 490

Query: 357  CEERREVEKALKHIRVFVCHRESVQR-YRVYGLTDEPTESLWF-ADRNGKNLRLVGYFKD 416
              +R +++KAL+ ++V V HR +++R YR+ GLT + T  L F  D  G    +V YF +
Sbjct: 491  DSDRVKIKKALRGVKVEVTHRGNMRRKYRISGLTSQATRELSFPVDDRGTVKTVVQYFLE 550

Query: 417  HYNYDIQYRNLPCLQI-SRSKPCYLPMELCMICEGQKFLGKLSDEQTARMLKMGCQRPKE 476
             Y + IQ+  LPCLQ+ ++ +P YLPME+C I EGQ++  +L+++Q   +LK+ CQRP+E
Sbjct: 551  TYGFSIQHTTLPCLQVGNQQRPNYLPMEVCKIVEGQRYSKRLNEKQITALLKVTCQRPQE 610

Query: 477  RKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRVLQPPKLKLGDGGQIRDLIPSR 536
            R+ +I   +            +EF +++   +  +  RVL PP+LK  D G+ +D++P  
Sbjct: 611  RELDILRTVSHN-AYHEDQYAQEFGIKIDERLASVEARVLPPPRLKYHDSGREKDVLP-- 670

Query: 537  HDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRFINQLSRRCEQLGIYLNSKTVV 596
               QWN+++  +  G R+  W+ I+F  +  Q      F ++L+  C+  G+    + V+
Sbjct: 671  RVGQWNMMNKKMVNGGRVNNWACINFSRNV-QDSAARGFCHELAIMCQISGMDFALEPVL 730

Query: 597  PP-QFEPTQVLNSVSLLESKLKKLHGAASNNLQLLICVM-ERKHKGYADLKRIAETSIGV 656
            PP    P  V  ++         +       L LLI ++ +     Y DLKRI ET +G+
Sbjct: 731  PPLTARPEHVERALKARYQDAMNMLRPQGRELDLLIVILPDNNGSLYGDLKRICETDLGL 790

Query: 657  VSQCCLYPNLAKLSSQFLANLALKINAKVGGCTVALYNSLPSQVPRLLRTDKPVIFMGAD 716
            VSQCCL  ++ K+S Q+LAN+ALKIN KVGG    L ++L  ++P  L +D+P I  GAD
Sbjct: 791  VSQCCLTKHVFKMSKQYLANVALKINVKVGGRNTVLVDALTRRIP--LVSDRPTIIFGAD 850

Query: 717  VTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMRSQTHRQEIIVDL-------------G 776
            VTHPHP +D SPS+AAVV S +WP   KY   + +Q HRQE+I DL             G
Sbjct: 851  VTHPHPGEDSSPSIAAVVASQDWPEVTKYAGLVSAQAHRQELIQDLFKVWQDPHRGTVTG 910

Query: 777  AMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKTACSRFH-NYKPPIT 836
             M++ELL  F +   + P RIIF+RDGVSE QFY+VL  EL AI+ AC+    NY+PP+T
Sbjct: 911  GMIKELLISFKRATGQKPQRIIFYRDGVSEGQFYQVLLYELDAIRKACASLEPNYQPPVT 970

Query: 837  FAVVQKRHHTRLFPFKVDPSSNHNE--VLDE--NIPPGTVVDTVITHPKEFDFYLCSHWG 896
            F VVQKRHHTRLF      ++NHN+   +D   NI PGTVVD+ I HP EFDFYLCSH G
Sbjct: 971  FVVVQKRHHTRLF------ANNHNDQRTVDRSGNILPGTVVDSKICHPTEFDFYLCSHAG 1030

Query: 897  VKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAHLAAYRGRL 952
            ++GTSRP HYHVL DEN+FT+DELQ L  NLCYTY RCT+ VS+VPPAYYAHLAA+R R 
Sbjct: 1031 IQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARF 1090

BLAST of Spg025899 vs. ExPASy Swiss-Prot
Match: Q6EU14 (Protein argonaute 1A OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1A PE=2 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.0e-179
Identity = 387/975 (39.69%), Postives = 556/975 (57.03%), Query Frame = 0

Query: 42   PPLAATSDTHVPILKISPAPKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEG- 101
            P L   S        ++P+P      SSLP++  + +V     E+ +  + P S  ++  
Sbjct: 140  PELHQASQDQYQATVVAPSPSRTG-PSSLPVEASSEEVQHQFQELAIQGQSPTSQAIQPA 199

Query: 102  ---------PVIP----------LLANHFLVQFDPSQRIYHYNVEISPN-PSKEVARMIK 161
                     P+ P          + ANHF  +  P + ++ Y+V I+P  PS+ V R + 
Sbjct: 200  PPSSKSVRFPMRPGKGTFGDRCIVKANHFFAEL-PDKDLHQYDVSITPEVPSRGVNRAVI 259

Query: 162  QKLV-EDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEGEIKHKQ 221
             ++V +     L G  P YDGRK+LY+          F V L      + V +G  + ++
Sbjct: 260  GEIVTQYRQSHLGGRLPVYDGRKSLYTAGPLPFTSRTFDVILQDEEESLAVGQGAQRRER 319

Query: 222  EFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENCFPVGRS 281
             FK   + IK  ++ D   L  +L     D    PQ+ L  LD+VLRE P     PV RS
Sbjct: 320  PFK---VVIKFAARADLHHLAMFLAGRQAD---APQEALQVLDIVLRELPTARYSPVARS 379

Query: 282  LYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIPYLQKRLDF 341
             YS ++   +++G G+   RGF+QS+RPTQ GL++N+D S TAF E + VI ++ + L+ 
Sbjct: 380  FYSPNLGRRQQLGEGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLN- 439

Query: 342  LRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQR-YRVYGLTDEPTESLWF-ADR 401
             RD+S    R L+  +R +++KAL+ ++V V HR +++R YR+ GLT + T  L F  D 
Sbjct: 440  -RDIS---VRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTSQATRELSFPIDN 499

Query: 402  NGKNLRLVGYFKDHYNYDIQYRNLPCLQI-SRSKPCYLPMELCMICEGQKFLGKLSDEQT 461
            +G    +V YF++ Y ++I++  LPCLQ+ ++ +P YLPME+C I EGQ++  +L+++Q 
Sbjct: 500  HGTVKTVVQYFQETYGFNIKHTTLPCLQVGNQQRPNYLPMEVCKIVEGQRYSKRLNEKQI 559

Query: 462  ARMLKMGCQRPKERKANI------DGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRVLQ 521
              +LK+ CQRP+ER+ +I      +   Q P         +EF +++   +  +  RVL 
Sbjct: 560  TALLKVTCQRPQERELDILQTVHHNAYHQDP-------YAQEFGIRIDERLASVEARVLP 619

Query: 522  PPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPR-FI 581
            PP LK  D G+ +D++P     QWN+++  +  G R+  W+ I+F  S   + N  R F 
Sbjct: 620  PPWLKYHDSGREKDVLP--RIGQWNMMNKKMVNGGRVNNWTCINF--SRHVQDNAARSFC 679

Query: 582  NQLSRRCEQLGIYLNSKTVVP-PQFEPTQVLNSVSLLESKLKKLHGAASNNLQLLICVM- 641
             +L+  C+  G+  +   VVP     P  V  ++     +   +       L LLI ++ 
Sbjct: 680  RELAIMCQISGMDFSIDPVVPLVTARPEHVERALKARYQEAMNILKPQGGELDLLIAILP 739

Query: 642  ERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTVALYNSL 701
            +     Y DLKRI ET +G+VSQCCL  ++ K+S Q+LAN+ALKIN KVGG    L ++L
Sbjct: 740  DNNGSLYGDLKRICETDLGLVSQCCLTKHVFKMSKQYLANVALKINVKVGGRNTVLVDAL 799

Query: 702  PSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMRSQTHRQ 761
              ++P  L +D+P I  GADVTHPHP +D SPS+AAVV S +WP   KY   + +Q HRQ
Sbjct: 800  TRRIP--LVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEVTKYAGLVSAQAHRQ 859

Query: 762  EIIVDL-------------GAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEE 821
            E+I DL             G M+ ELL  F +   + P RIIF+RDGVSE QFY+VL  E
Sbjct: 860  ELIQDLFKVWKDPQRGTVSGGMIRELLISFKRATGQKPQRIIFYRDGVSEGQFYQVLFYE 919

Query: 822  LQAIKTACSRFH-NYKPPITFAVVQKRHHTRLFPFKVDPSSNHNE--VLDE--NIPPGTV 881
            L AI+ AC+    +Y+PP+TF VVQKRHHTRLF      ++NH +   +D   NI PGTV
Sbjct: 920  LDAIRKACASLEADYQPPVTFVVVQKRHHTRLF------ANNHKDQRTVDRSGNILPGTV 979

Query: 882  VDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTK 941
            VD+ I HP EFDFYLCSH G++GTSRP HYHVL DEN+FT+D LQ L  NLCYTY RCT+
Sbjct: 980  VDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADGLQTLTNNLCYTYARCTR 1039

Query: 942  PVSLVPPAYYAHLAAYRGRLYLE--RSD-----STAHTRGVSTVSRAAPPK------TMP 952
             VS+VPPAYYAHLAA+R R Y+E   SD     S AHTRG   +  A   K        P
Sbjct: 1040 SVSIVPPAYYAHLAAFRARFYMEPDTSDSGSMASGAHTRGGGPLPGARSTKPAGNVAVRP 1082

BLAST of Spg025899 vs. ExPASy Swiss-Prot
Match: Q7Y001 (Protein argonaute 12 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO12 PE=2 SV=2)

HSP 1 Score: 627.1 bits (1616), Expect = 3.3e-178
Identity = 368/905 (40.66%), Postives = 521/905 (57.57%), Query Frame = 0

Query: 82   SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPNPS-KEVARMIK 141
            S  + +    RPD G + G    + ANHFLVQ    + IYHY+V I+P  + +E  R I 
Sbjct: 186  SSSKAVTFPARPDVGTI-GRRCRVRANHFLVQV-ADKDIYHYDVVITPESTYRERNRSII 245

Query: 142  QKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEGEIKH--- 201
             KLV  +   L G  P YDGRK++Y+             + P+P    K  +  +KH   
Sbjct: 246  NKLVALHKQFLDGRLPVYDGRKSIYT-------------AGPLP---FKTKDFVVKHINP 305

Query: 202  ---KQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGP---NE 261
                Q  + ++++IK  SK D   L ++L     +   +PQD + ALD+ LRE P   N 
Sbjct: 306  LRGNQREEEYKVTIKQASKTDLYSLKQFLVGRQRE---LPQDTIQALDIALRECPTSVNF 365

Query: 262  NC---FPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIG 321
             C     + RS +S S     EIG G    RG++QSLRPTQ GL++N+D S TAF+++  
Sbjct: 366  TCDRYVSISRSFFSQSFGHGGEIGSGTECWRGYYQSLRPTQMGLSLNIDISATAFYKAQP 425

Query: 322  VIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCH-RESVQRYRVYGLTDE 381
            V+ +  + L+ +RD+S+R    L+ ++R +++KALK +++   H +E   RY++ G+   
Sbjct: 426  VMDFAVQYLN-IRDVSRR----LSDQDRIKLKKALKGVQIVATHWKEKSIRYKITGIPSA 485

Query: 382  PTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQI-SRSKPCYLPMELCMICEGQK 441
            P   L F D +G  + +V YFK  YNY +++ N PCLQ  S S+P YLPME+C I EGQ+
Sbjct: 486  PMNELMF-DLDGNRISVVQYFKKQYNYSLKHVNWPCLQAGSDSRPKYLPMEVCSILEGQR 545

Query: 442  FLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSG 501
            +  KL++ Q   +L+M C+RP +R+++I  ++            +EF ++V+ ++  +  
Sbjct: 546  YSKKLNEHQVTNILRMTCERPAQRESSIIEIVNTNSYGNDDC-AKEFGIKVANQLAVVDA 605

Query: 502  RVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIP 561
            RVL  P+LK  D G+ +   PS    QWN+++  +  G  I  W+ +SF        +I 
Sbjct: 606  RVLPTPRLKYHDSGREKVCNPS--VGQWNMINKRMVNGGCINHWTCLSFASRMHVN-DIR 665

Query: 562  RFINQLSRRCEQLGIYLNSKTVVP----PQFEPTQVLNSVSLLESKLKKLHGAASNNLQL 621
             F   L   C  +G+ +N++  V      Q      + ++    S+           LQL
Sbjct: 666  MFCEDLVGMCNNIGMQMNTRPCVDIIQGQQRNIEGAIRNIHRQSSEKLDQQDLTGQQLQL 725

Query: 622  LICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTVA 681
            LI ++      Y  +KRI ET +GV++QCC   +L K   Q+L NLALK+N KVGG    
Sbjct: 726  LIVILTEISGSYGRIKRICETEVGVITQCCAPKSLQKGGKQYLENLALKMNVKVGGRNTV 785

Query: 682  LYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMRS 741
            L ++L  ++P L  TD+P I  GADVTHP P +D SPS+AAVV SM+WP   KY   + +
Sbjct: 786  LEDALHKKIPIL--TDRPTIVFGADVTHPSPGEDASPSIAAVVASMDWPEVTKYKCLVST 845

Query: 742  QTHRQEIIVDL-------------GAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYK 801
            Q+HR+EII +L             G M+ ELL  FYQE  + PSRIIF+RDG+SE QF +
Sbjct: 846  QSHREEIISNLYTEVKDPLKGIIRGGMIRELLRSFYQETGQKPSRIIFYRDGISEGQFSQ 905

Query: 802  VLQEELQAIKTACSRF-HNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDE--NIPP 861
            VL  E+ AI+ AC+     Y PP+TF VVQKRHHTRLFP       N  +++D   NI P
Sbjct: 906  VLLYEMDAIRKACASLQEGYLPPVTFVVVQKRHHTRLFP------ENRRDMMDRSGNILP 965

Query: 862  GTVVDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNR 921
            GTVVDT+I HP EFDFYLCSH G+KGTSRPTHYHVL DEN F +D LQ L YNL YTY R
Sbjct: 966  GTVVDTMICHPSEFDFYLCSHSGIKGTSRPTHYHVLLDENGFKADTLQTLTYNLSYTYAR 1025

Query: 922  CTKPVSLVPPAYYAHLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRK 952
            CT+ VS+VPPAYYAHL A+R R Y+E  D  +     S+V+      T PLP++ ENV++
Sbjct: 1026 CTRAVSIVPPAYYAHLGAFRARYYME--DEHSDQGSSSSVTTRTDRSTKPLPEIKENVKR 1049

BLAST of Spg025899 vs. ExPASy TrEMBL
Match: A0A5A7SUX2 (Protein argonaute 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00120 PE=3 SV=1)

HSP 1 Score: 1852.4 bits (4797), Expect = 0.0e+00
Identity = 905/951 (95.16%), Postives = 928/951 (97.58%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MKVSEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKVSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EER+EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEERKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWGLISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRMDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST HTRG+STVSRAAPPKTMPLPKL+ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTHTRGISTVSRAAPPKTMPLPKLNENVRKLMFYC 1019

BLAST of Spg025899 vs. ExPASy TrEMBL
Match: A0A1S3BFT3 (protein argonaute 7 OS=Cucumis melo OX=3656 GN=LOC103489157 PE=3 SV=1)

HSP 1 Score: 1850.1 bits (4791), Expect = 0.0e+00
Identity = 904/951 (95.06%), Postives = 927/951 (97.48%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MKVSEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKVSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EER+EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEERKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RM KMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMQKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWGLISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRMDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST HTRG+STVSRAAPPKTMPLPKL+ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTHTRGISTVSRAAPPKTMPLPKLNENVRKLMFYC 1019

BLAST of Spg025899 vs. ExPASy TrEMBL
Match: A0A6J1DE07 (protein argonaute 7 OS=Momordica charantia OX=3673 GN=LOC111019210 PE=3 SV=1)

HSP 1 Score: 1849.7 bits (4790), Expect = 0.0e+00
Identity = 906/951 (95.27%), Postives = 928/951 (97.58%), Query Frame = 0

Query: 2    LPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPAP 61
            LPLPLPPPIPLQLALAP LPPN TT  KTH QKPSCRLN PPLAA SDTH+PI+KISPAP
Sbjct: 73   LPLPLPPPIPLQLALAP-LPPNTTTTTKTHFQKPSCRLNTPPLAAPSDTHLPIIKISPAP 132

Query: 62   KGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRIY 121
            KGLRLKS+LPLKGENGKVSESKPE LVAARRPDSGGVEGP IPLLANHFLVQFDPSQRI 
Sbjct: 133  KGLRLKSNLPLKGENGKVSESKPEALVAARRPDSGGVEGPFIPLLANHFLVQFDPSQRIC 192

Query: 122  HYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSL 181
            HYNVEISPNPSKE+ARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFY+SL
Sbjct: 193  HYNVEISPNPSKEIARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYISL 252

Query: 182  PIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHAL 241
            PIPS+KMKV+ GEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDW PIPQDYLHAL
Sbjct: 253  PIPSNKMKVTGGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWTPIPQDYLHAL 312

Query: 242  DVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT 301
            DVVLRE PNE CFPVGRSLYSSSMWG K+IGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT
Sbjct: 313  DVVLRETPNEKCFPVGRSLYSSSMWGGKDIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVT 372

Query: 302  AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV 361
            AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV
Sbjct: 373  AFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRV 432

Query: 362  YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCMI 421
            YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNY+IQ+RNLPCLQISRSKPCYLPMELCMI
Sbjct: 433  YGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYEIQFRNLPCLQISRSKPCYLPMELCMI 492

Query: 422  CEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREM 481
            CEGQ+FLGKLSDEQTARMLKMGCQRP+ERKANIDGVMQGPVGPTSG QGREF+LQVSREM
Sbjct: 493  CEGQRFLGKLSDEQTARMLKMGCQRPRERKANIDGVMQGPVGPTSGNQGREFSLQVSREM 552

Query: 482  TKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQ 541
            TKL+GRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW+LISFGGS DQ
Sbjct: 553  TKLTGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWALISFGGSLDQ 612

Query: 542  RLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNLQ 601
            RLNIPRFINQLSRRCEQLGI+LNSK VVPPQFEPTQVLNSVS+LESKLKKLHGAASN L+
Sbjct: 613  RLNIPRFINQLSRRCEQLGIFLNSKPVVPPQFEPTQVLNSVSILESKLKKLHGAASNKLE 672

Query: 602  LLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV 661
            LLICVMERKHKGYADLKRIAETSIG+VSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV
Sbjct: 673  LLICVMERKHKGYADLKRIAETSIGIVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTV 732

Query: 662  ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMR 721
            ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRMR
Sbjct: 733  ALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRMR 792

Query: 722  SQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKTA 781
            SQTHRQEIIVDLGAMVEELL+EFYQEVNELPSRIIFFRDGVSETQF+KVLQEELQAIKTA
Sbjct: 793  SQTHRQEIIVDLGAMVEELLDEFYQEVNELPSRIIFFRDGVSETQFFKVLQEELQAIKTA 852

Query: 782  CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEFD 841
            CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSN NEVLDENIPPGTVVDTVITHPKEFD
Sbjct: 853  CSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNQNEVLDENIPPGTVVDTVITHPKEFD 912

Query: 842  FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH 901
            FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH
Sbjct: 913  FYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAH 972

Query: 902  LAAYRGRLYLERSD-STAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            LAAYRGRLYLERSD STAHTRGVS VSRAAPPKT PLPKLSENVRKLMFYC
Sbjct: 973  LAAYRGRLYLERSDYSTAHTRGVSAVSRAAPPKTTPLPKLSENVRKLMFYC 1022

BLAST of Spg025899 vs. ExPASy TrEMBL
Match: A0A0A0KQR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G606440 PE=3 SV=1)

HSP 1 Score: 1840.5 bits (4766), Expect = 0.0e+00
Identity = 900/951 (94.64%), Postives = 925/951 (97.27%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLP PIPLQL    ALPP++TTRPK HLQKPSC+L+NPPLAATSDTHVPI+KISPA
Sbjct: 73   PLPLPLPSPIPLQL----ALPPSKTTRPKPHLQKPSCKLSNPPLAATSDTHVPIIKISPA 132

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
            P+GLRLKSSLPLKGENGKVS+SKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI
Sbjct: 133  PEGLRLKSSLPLKGENGKVSQSKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 192

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS
Sbjct: 193  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 252

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSS+MK SEGEI+HKQ FKLFRISIKLVSKFDGMELNRY+TKEGDDWIPIPQDYLHA
Sbjct: 253  LPIPSSRMKFSEGEIEHKQAFKLFRISIKLVSKFDGMELNRYMTKEGDDWIPIPQDYLHA 312

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVVLREGPNE C PVGRSLYSSSMWG KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 313  LDVVLREGPNEKCIPVGRSLYSSSMWGGKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 372

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLT EE++EVEKAL HIRVFVCHRESVQRYR
Sbjct: 373  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTIEEKKEVEKALMHIRVFVCHRESVQRYR 432

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQ+RNLPCLQISRSKPCYLPMELCM
Sbjct: 433  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQFRNLPCLQISRSKPCYLPMELCM 492

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKL+DEQT RMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREF+LQVS+E
Sbjct: 493  ICEGQKFLGKLTDEQTTRMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFSLQVSKE 552

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKLSGRVLQPPKLKLG+GGQIRDLIPSRHDRQWNLVDCHVFEGTRIERW+LISFGG PD
Sbjct: 553  MTKLSGRVLQPPKLKLGEGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWALISFGGVPD 612

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QR NIPRFINQLSRRC+QLGI+LNSKTVVPPQFE TQVLNSVSLLESKLKK+HGAASNNL
Sbjct: 613  QRSNIPRFINQLSRRCDQLGIFLNSKTVVPPQFESTQVLNSVSLLESKLKKIHGAASNNL 672

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 673  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 732

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSL SQVPRLLR DKPVIFMGADVTHPHPLDDFSPS+AAVVGSMNWPAANKYVSRM
Sbjct: 733  VALYNSLHSQVPRLLRIDKPVIFMGADVTHPHPLDDFSPSIAAVVGSMNWPAANKYVSRM 792

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLG MVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT
Sbjct: 793  RSQTHRQEIIVDLGTMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 852

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRF NYKPPITFAVVQKRHHTRLFPFKVDPSSN  EVLDENIPPGTVVDTVITHPKEF
Sbjct: 853  ACSRFLNYKPPITFAVVQKRHHTRLFPFKVDPSSNQTEVLDENIPPGTVVDTVITHPKEF 912

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYH+LCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 913  DFYLCSHWGVKGTSRPTHYHILCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 972

Query: 901  HLAAYRGRLYLERSDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSDST +TRG+STVSRAAPPKT PLPKL ENVRKLMFYC
Sbjct: 973  HLAAYRGRLYLERSDSTTYTRGISTVSRAAPPKTTPLPKLKENVRKLMFYC 1019

BLAST of Spg025899 vs. ExPASy TrEMBL
Match: A0A6J1HW90 (protein argonaute 7-like OS=Cucurbita maxima OX=3661 GN=LOC111467438 PE=3 SV=1)

HSP 1 Score: 1820.4 bits (4714), Expect = 0.0e+00
Identity = 891/952 (93.59%), Postives = 918/952 (96.43%), Query Frame = 0

Query: 1    PLPLPLPPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTHVPILKISPA 60
            PLPLPLPPPIPLQLALAP LP NR TRPK H QKPSC+LNNPPLAAT+DTHVPILKISPA
Sbjct: 69   PLPLPLPPPIPLQLALAPPLPTNRATRPKPHSQKPSCKLNNPPLAATADTHVPILKISPA 128

Query: 61   PKGLRLKSSLPLKGENGKVSESKPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRI 120
             KGLRLKSSLP KG NGKVSESKPEVLVAA RPDSGGVEGPVIPLLANHFLV+FDPSQRI
Sbjct: 129  SKGLRLKSSLPHKGGNGKVSESKPEVLVAASRPDSGGVEGPVIPLLANHFLVKFDPSQRI 188

Query: 121  YHYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVS 180
            YHYNVEISPNPSKEVARMIKQKLVE+NSDLLSGASPAYDGRKNLYSPIEFV+DRLEFYVS
Sbjct: 189  YHYNVEISPNPSKEVARMIKQKLVEENSDLLSGASPAYDGRKNLYSPIEFVQDRLEFYVS 248

Query: 181  LPIPSSKMKVSEGEIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHA 240
            LPIPSSKMKV  GEI+HKQEFKLFRISIKLVSKFDGMELNRYLT EGDDWIPIPQDYLHA
Sbjct: 249  LPIPSSKMKVPGGEIQHKQEFKLFRISIKLVSKFDGMELNRYLTNEGDDWIPIPQDYLHA 308

Query: 241  LDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 300
            LDVV+REGPNE C  VGRSLYSSS WG+KEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV
Sbjct: 309  LDVVMREGPNEKCITVGRSLYSSSAWGAKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSV 368

Query: 301  TAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYR 360
            TAFHESIGVIPYLQKRLD LRDL+QRKTRGL  EERREVEKALKHIRVFVCHRESVQRYR
Sbjct: 369  TAFHESIGVIPYLQKRLDCLRDLTQRKTRGLNIEERREVEKALKHIRVFVCHRESVQRYR 428

Query: 361  VYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 420
            VYGLTDEPTE LWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM
Sbjct: 429  VYGLTDEPTEGLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPMELCM 488

Query: 421  ICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSRE 480
            ICEGQKFLGKLSD+QTARMLKMGCQRPKERKANIDGVMQGP+GP SG+ GREF+L+VSRE
Sbjct: 489  ICEGQKFLGKLSDKQTARMLKMGCQRPKERKANIDGVMQGPLGPNSGIHGREFSLEVSRE 548

Query: 481  MTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPD 540
            MTKL+GRVLQPPKLKLGDGGQIRDLIPSR DRQWNLVDCHVFEGTRIERW LISF  SPD
Sbjct: 549  MTKLNGRVLQPPKLKLGDGGQIRDLIPSRPDRQWNLVDCHVFEGTRIERWGLISFSSSPD 608

Query: 541  QRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNL 600
            QRL+IPRFINQLSRRCEQLGI+LNSKT+VPPQFEPTQVLNSVSLLESKLK +HGAASNNL
Sbjct: 609  QRLSIPRFINQLSRRCEQLGIFLNSKTIVPPQFEPTQVLNSVSLLESKLKMIHGAASNNL 668

Query: 601  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 660
            QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT
Sbjct: 669  QLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 728

Query: 661  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 720
            VALYNSLPSQVPRLL+TDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKY SRM
Sbjct: 729  VALYNSLPSQVPRLLQTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYASRM 788

Query: 721  RSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKT 780
            RSQTHRQEIIVDLGAMVEELLEEFYQE+NELP RIIFFRDGVSETQFYKVLQ+ELQAIK 
Sbjct: 789  RSQTHRQEIIVDLGAMVEELLEEFYQELNELPRRIIFFRDGVSETQFYKVLQKELQAIKA 848

Query: 781  ACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHPKEF 840
            ACSRFHNYKPPITFAVVQKRHHTRLFPFK+DPSSN +EVLDENIPPGTVVD+VITHPKEF
Sbjct: 849  ACSRFHNYKPPITFAVVQKRHHTRLFPFKIDPSSNQSEVLDENIPPGTVVDSVITHPKEF 908

Query: 841  DFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 900
            DFYLCSHWGVKGTSRPTHYHVLCDEN FTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA
Sbjct: 909  DFYLCSHWGVKGTSRPTHYHVLCDENHFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYA 968

Query: 901  HLAAYRGRLYLERSD-STAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
            HLAAYRGRLYLERSD S AHTRGV+TVSRAAPPKTMPLPKLSENV+KLMFYC
Sbjct: 969  HLAAYRGRLYLERSDYSMAHTRGVNTVSRAAPPKTMPLPKLSENVKKLMFYC 1020

BLAST of Spg025899 vs. TAIR 10
Match: AT1G69440.1 (Argonaute family protein )

HSP 1 Score: 1262.7 bits (3266), Expect = 0.0e+00
Identity = 636/955 (66.60%), Postives = 756/955 (79.16%), Query Frame = 0

Query: 7   PPPIPLQLALAPALPPNRTTRPKTHLQKPSCRLNNPPLAATSDTH--VPILKISPAPKGL 66
           PPP P  L L+P LPP                L  PP  + +  H  +P+ ++    +  
Sbjct: 80  PPPPPHLLPLSPPLPP---------------LLPLPPPHSMTRFHKSLPVSQVVERKQQH 139

Query: 67  RLKSSLPLKGENGKVSES---KPEVLVAARRPDSGGVEGPVIPLLANHFLVQFDPSQRIY 126
           + K  + +   N KVS S   +   LV A+RPD GG +G VI LLANHFLV+FD SQRIY
Sbjct: 140 QQKKKIQV--SNNKVSGSIAIEEAALVVAKRPDFGGQDGSVIYLLANHFLVKFDSSQRIY 199

Query: 127 HYNVEISPNPSKEVARMIKQKLVEDNSDLLSGASPAYDGRKNLYSPIEFVRDRLEFYVSL 186
           HYNVEISP PSKE+ARMIKQKLVE + +  SG  PA+DGR+N+YSP+EF  DRLEF+V+L
Sbjct: 200 HYNVEISPQPSKEIARMIKQKLVETDRNSFSGVVPAFDGRQNIYSPVEFQGDRLEFFVNL 259

Query: 187 PIPSSKMKVSEGEIKHKQE----FKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDY 246
           PIPS K  ++ G+++ KQ      KLFR+++KLVSKFDG E      KEG+DW P+P +Y
Sbjct: 260 PIPSCKAVMNYGDLREKQPQKKIEKLFRVNMKLVSKFDGKEQR----KEGEDWAPLPPEY 319

Query: 247 LHALDVVLREGPNENCFPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVD 306
           +HALDV+LRE P E C  +GRS YSSSM GSKEIGGG VGLRGFFQSLR TQQGLA+N+D
Sbjct: 320 IHALDVILRENPMEKCTSIGRSFYSSSMGGSKEIGGGAVGLRGFFQSLRHTQQGLALNMD 379

Query: 307 CSVTAFHESIGVIPYLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQ 366
            S+TAFHESIGVI YLQKRL+FL DL + K R L+ EE+REVEKALK+IRVFVCHRE+VQ
Sbjct: 380 LSITAFHESIGVIAYLQKRLEFLTDLPRNKGRELSLEEKREVEKALKNIRVFVCHRETVQ 439

Query: 367 RYRVYGLTDEPTESLWFADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRSKPCYLPME 426
           RYRVYGLT+E TE++WF DR GK LRL+ YFKDHY Y+IQ++NLPCLQISR++PCYLPME
Sbjct: 440 RYRVYGLTEEITENIWFPDREGKYLRLMSYFKDHYGYEIQFKNLPCLQISRARPCYLPME 499

Query: 427 LCMICEGQKFLGKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQV 486
           LCMICEGQKFLGKLSD+Q A+++KMGCQ+P ERKA ID VM G VGP+SG Q REFNL+V
Sbjct: 500 LCMICEGQKFLGKLSDDQAAKIMKMGCQKPNERKAIIDKVMTGSVGPSSGNQTREFNLEV 559

Query: 487 SREMTKLSGRVLQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGG 546
           SREMT L GR+LQPPKLKL              DR  NL +  VF+GTRIERW+L+S GG
Sbjct: 560 SREMTLLKGRILQPPKLKL--------------DRPRNLKESKVFKGTRIERWALMSIGG 619

Query: 547 SPDQRLNIPRFINQLSRRCEQLGIYLNSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAAS 606
           S DQ+  IP+FIN+L+++CE LG++L+  T+    FEP+ +LN++SLLESKLK++  AAS
Sbjct: 620 SSDQKSTIPKFINELTQKCEHLGVFLSKNTLSSTFFEPSHILNNISLLESKLKEIQRAAS 679

Query: 607 NNLQLLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVG 666
           NNLQL+ICVME+KHKGY DLKRI+ET IGVV+QCCLYPN+ KLSSQF++NLALKINAK+G
Sbjct: 680 NNLQLIICVMEKKHKGYGDLKRISETRIGVVTQCCLYPNITKLSSQFVSNLALKINAKIG 739

Query: 667 GCTVALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYV 726
           G    LYNS+PS +PRLLR D+PVIFMGADVTHPHP DD SPSVAAVVGS+NWP AN+YV
Sbjct: 740 GSMTELYNSIPSHIPRLLRPDEPVIFMGADVTHPHPFDDCSPSVAAVVGSINWPEANRYV 799

Query: 727 SRMRSQTHRQEIIVDLGAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYKVLQEELQA 786
           SRMRSQTHRQEII DL  MV+ELL++FY+ V +LP+RIIFFRDGVSETQF KVLQEELQ+
Sbjct: 800 SRMRSQTHRQEIIQDLDLMVKELLDDFYKAVKKLPNRIIFFRDGVSETQFKKVLQEELQS 859

Query: 787 IKTACSRFHNYKPPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDENIPPGTVVDTVITHP 846
           IKTACS+F +Y P ITFAVVQKRHHTRL  F+ DP         ENIPPGTVVDTVITHP
Sbjct: 860 IKTACSKFQDYNPSITFAVVQKRHHTRL--FRCDPD-------HENIPPGTVVDTVITHP 919

Query: 847 KEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPA 906
           KEFDFYLCSH GVKGTSRPTHYH+L DEN+FTSDELQ+LVYNLCYT+ RCTKP+S+VPPA
Sbjct: 920 KEFDFYLCSHLGVKGTSRPTHYHILWDENEFTSDELQRLVYNLCYTFVRCTKPISIVPPA 979

Query: 907 YYAHLAAYRGRLYLER-SDSTAHTRGVSTVSRAAPPKTMPLPKLSENVRKLMFYC 952
           YYAHLAAYRGRLY+ER S+S   +   S+VSR  PPKT+PLPKLS+NV+ LMFYC
Sbjct: 980 YYAHLAAYRGRLYIERSSESNGGSMNPSSVSRVGPPKTIPLPKLSDNVKNLMFYC 990

BLAST of Spg025899 vs. TAIR 10
Match: AT1G48410.1 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 625.5 bits (1612), Expect = 6.8e-179
Identity = 393/979 (40.14%), Postives = 551/979 (56.28%), Query Frame = 0

Query: 21   PPNRTTRPKTH-LQKPSCRL--NNPPLAATSDTHVPILKISPAPKGLRLKSSLPLKGENG 80
            PP R + P+ H    P+ +   + P L+  S T VP     P     + +     +G   
Sbjct: 109  PPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVP----EPTVLAQQFEQLSVEQGAPS 168

Query: 81   KVSESKPEVLVAARRP--DSGGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPN-PSKE 140
            +  +  P    A + P     G  G    + ANHF  +  P + ++HY+V I+P   S+ 
Sbjct: 169  QAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAEL-PDKDLHHYDVTITPEVTSRG 228

Query: 141  VARMIKQKLVEDNSDL-LSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEG 200
            V R + ++LV++  D  L    PAYDGRK+LY+      +  EF ++L       +V  G
Sbjct: 229  VNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINL----LDEEVGAG 288

Query: 201  EIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENC 260
              + ++EFK   + IKLV++ D   L  +L  +  D    PQ+ L  LD+VLRE P    
Sbjct: 289  GQRREREFK---VVIKLVARADLHHLGMFLEGKQSD---APQEALQVLDIVLRELPTSRY 348

Query: 261  FPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIPYL 320
             PVGRS YS  +   + +G G+   RGF+QS+RPTQ GL++N+D S TAF E+  VI ++
Sbjct: 349  IPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQFV 408

Query: 321  QKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQR-YRVYGLTDEPTESL 380
               L+  RD+S   +R L+  +R +++KAL+ ++V V HR +++R YR+ GLT   T  L
Sbjct: 409  CDLLN--RDIS---SRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATREL 468

Query: 381  WF-ADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRS-KPCYLPMELCMICEGQKFLGK 440
             F  D       +V YF + Y + IQ+  LPCLQ+  S +P YLPME+C I EGQ++  +
Sbjct: 469  TFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYSKR 528

Query: 441  LSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRVLQ 500
            L++ Q   +LK+ CQRP +R+ +I   +Q           +EF +++S  +  +  R+L 
Sbjct: 529  LNERQITALLKVTCQRPIDREKDILQTVQ-LNDYAKDNYAQEFGIKISTSLASVEARILP 588

Query: 501  PPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRFIN 560
            PP LK  + G+    +P     QWN+++  +  G  +  W  I+F       L    F  
Sbjct: 589  PPWLKYHESGREGTCLP--QVGQWNMMNKKMINGGTVNNWICINFSRQVQDNL-ARTFCQ 648

Query: 561  QLSRRCEQLGIYLNSKTVVPP-QFEPTQVLNSVSLLESKLK-KLHGAASNNLQ------L 620
            +L++ C   G+  N + V+PP    P QV       E  LK + H A S   Q      L
Sbjct: 649  ELAQMCYVSGMAFNPEPVLPPVSARPEQV-------EKVLKTRYHDATSKLSQGKEIDLL 708

Query: 621  LICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTVA 680
            ++ + +     Y DLKRI ET +G+VSQCCL  ++ K+S Q++AN+ALKIN KVGG    
Sbjct: 709  IVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRNTV 768

Query: 681  LYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMRS 740
            L ++L  ++P  L +D+P I  GADVTHPHP +D SPS+AAVV S +WP   KY   + +
Sbjct: 769  LVDALSRRIP--LVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLVCA 828

Query: 741  QTHRQEIIVDL-------------GAMVEELLEEFYQEVNELPSRIIFFRDGVSETQFYK 800
            Q HRQE+I DL             G M++ELL  F +     P RIIF+RDGVSE QFY+
Sbjct: 829  QAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQFYQ 888

Query: 801  VLQEELQAIKTACSRFH-NYKPPITFAVVQKRHHTRLFPFKVDPSSNHNE--VLDE--NI 860
            VL  EL AI+ AC+     Y+PP+TF VVQKRHHTRLF      + NHN+   +D   NI
Sbjct: 889  VLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLF------AQNHNDRHSVDRSGNI 948

Query: 861  PPGTVVDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTY 920
             PGTVVD+ I HP EFDFYLCSH G++GTSRP HYHVL DEN FT+D LQ L  NLCYTY
Sbjct: 949  LPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCYTY 1008

Query: 921  NRCTKPVSLVPPAYYAHLAAYRGRLYLE---------RSDSTAHTRGVSTVSRAAP---P 952
             RCT+ VS+VPPAYYAHLAA+R R Y+E          S S A   G++  S   P    
Sbjct: 1009 ARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNVNA 1048

BLAST of Spg025899 vs. TAIR 10
Match: AT1G48410.2 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 620.9 bits (1600), Expect = 1.7e-177
Identity = 393/981 (40.06%), Postives = 551/981 (56.17%), Query Frame = 0

Query: 21   PPNRTTRPKTH-LQKPSCRL--NNPPLAATSDTHVPILKISPAPKGLRLKSSLPLKGENG 80
            PP R + P+ H    P+ +   + P L+  S T VP     P     + +     +G   
Sbjct: 109  PPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVP----EPTVLAQQFEQLSVEQGAPS 168

Query: 81   KVSESKPEVLVAARRP--DSGGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPN-PSKE 140
            +  +  P    A + P     G  G    + ANHF  +  P + ++HY+V I+P   S+ 
Sbjct: 169  QAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAEL-PDKDLHHYDVTITPEVTSRG 228

Query: 141  VARMIKQKLVEDNSDL-LSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEG 200
            V R + ++LV++  D  L    PAYDGRK+LY+      +  EF ++L       +V  G
Sbjct: 229  VNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINL----LDEEVGAG 288

Query: 201  EIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENC 260
              + ++EFK   + IKLV++ D   L  +L  +  D    PQ+ L  LD+VLRE P    
Sbjct: 289  GQRREREFK---VVIKLVARADLHHLGMFLEGKQSD---APQEALQVLDIVLRELPTSRI 348

Query: 261  --FPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIP 320
               PVGRS YS  +   + +G G+   RGF+QS+RPTQ GL++N+D S TAF E+  VI 
Sbjct: 349  RYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQ 408

Query: 321  YLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQR-YRVYGLTDEPTE 380
            ++   L+  RD+S   +R L+  +R +++KAL+ ++V V HR +++R YR+ GLT   T 
Sbjct: 409  FVCDLLN--RDIS---SRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATR 468

Query: 381  SLWF-ADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRS-KPCYLPMELCMICEGQKFL 440
             L F  D       +V YF + Y + IQ+  LPCLQ+  S +P YLPME+C I EGQ++ 
Sbjct: 469  ELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYS 528

Query: 441  GKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRV 500
             +L++ Q   +LK+ CQRP +R+ +I   +Q           +EF +++S  +  +  R+
Sbjct: 529  KRLNERQITALLKVTCQRPIDREKDILQTVQ-LNDYAKDNYAQEFGIKISTSLASVEARI 588

Query: 501  LQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRF 560
            L PP LK  + G+    +P     QWN+++  +  G  +  W  I+F       L    F
Sbjct: 589  LPPPWLKYHESGREGTCLP--QVGQWNMMNKKMINGGTVNNWICINFSRQVQDNL-ARTF 648

Query: 561  INQLSRRCEQLGIYLNSKTVVPP-QFEPTQVLNSVSLLESKLK-KLHGAASNNLQ----- 620
              +L++ C   G+  N + V+PP    P QV       E  LK + H A S   Q     
Sbjct: 649  CQELAQMCYVSGMAFNPEPVLPPVSARPEQV-------EKVLKTRYHDATSKLSQGKEID 708

Query: 621  -LLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 680
             L++ + +     Y DLKRI ET +G+VSQCCL  ++ K+S Q++AN+ALKIN KVGG  
Sbjct: 709  LLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRN 768

Query: 681  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 740
              L ++L  ++P  L +D+P I  GADVTHPHP +D SPS+AAVV S +WP   KY   +
Sbjct: 769  TVLVDALSRRIP--LVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLV 828

Query: 741  RSQTHRQEIIVDL-------------GAMVEELLEEFYQEVNELPSRIIFFRDGVSETQF 800
             +Q HRQE+I DL             G M++ELL  F +     P RIIF+RDGVSE QF
Sbjct: 829  CAQAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQF 888

Query: 801  YKVLQEELQAIKTACSRFH-NYKPPITFAVVQKRHHTRLFPFKVDPSSNHNE--VLDE-- 860
            Y+VL  EL AI+ AC+     Y+PP+TF VVQKRHHTRLF      + NHN+   +D   
Sbjct: 889  YQVLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLF------AQNHNDRHSVDRSG 948

Query: 861  NIPPGTVVDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCY 920
            NI PGTVVD+ I HP EFDFYLCSH G++GTSRP HYHVL DEN FT+D LQ L  NLCY
Sbjct: 949  NILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCY 1008

Query: 921  TYNRCTKPVSLVPPAYYAHLAAYRGRLYLE---------RSDSTAHTRGVSTVSRAAP-- 952
            TY RCT+ VS+VPPAYYAHLAA+R R Y+E          S S A   G++  S   P  
Sbjct: 1009 TYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNV 1050

BLAST of Spg025899 vs. TAIR 10
Match: AT1G48410.3 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 620.9 bits (1600), Expect = 1.7e-177
Identity = 393/981 (40.06%), Postives = 551/981 (56.17%), Query Frame = 0

Query: 21   PPNRTTRPKTH-LQKPSCRL--NNPPLAATSDTHVPILKISPAPKGLRLKSSLPLKGENG 80
            PP R + P+ H    P+ +   + P L+  S T VP     P     + +     +G   
Sbjct: 109  PPQRQSVPELHQATSPTYQAVSSQPTLSEVSPTQVP----EPTVLAQQFEQLSVEQGAPS 168

Query: 81   KVSESKPEVLVAARRP--DSGGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPN-PSKE 140
            +  +  P    A + P     G  G    + ANHF  +  P + ++HY+V I+P   S+ 
Sbjct: 169  QAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAEL-PDKDLHHYDVTITPEVTSRG 228

Query: 141  VARMIKQKLVEDNSDL-LSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEG 200
            V R + ++LV++  D  L    PAYDGRK+LY+      +  EF ++L       +V  G
Sbjct: 229  VNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINL----LDEEVGAG 288

Query: 201  EIKHKQEFKLFRISIKLVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENC 260
              + ++EFK   + IKLV++ D   L  +L  +  D    PQ+ L  LD+VLRE P    
Sbjct: 289  GQRREREFK---VVIKLVARADLHHLGMFLEGKQSD---APQEALQVLDIVLRELPTSRI 348

Query: 261  --FPVGRSLYSSSMWGSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIP 320
               PVGRS YS  +   + +G G+   RGF+QS+RPTQ GL++N+D S TAF E+  VI 
Sbjct: 349  RYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEANPVIQ 408

Query: 321  YLQKRLDFLRDLSQRKTRGLTCEERREVEKALKHIRVFVCHRESVQR-YRVYGLTDEPTE 380
            ++   L+  RD+S   +R L+  +R +++KAL+ ++V V HR +++R YR+ GLT   T 
Sbjct: 409  FVCDLLN--RDIS---SRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTAVATR 468

Query: 381  SLWF-ADRNGKNLRLVGYFKDHYNYDIQYRNLPCLQISRS-KPCYLPMELCMICEGQKFL 440
             L F  D       +V YF + Y + IQ+  LPCLQ+  S +P YLPME+C I EGQ++ 
Sbjct: 469  ELTFPVDERNTQKSVVEYFHETYGFRIQHTQLPCLQVGNSNRPNYLPMEVCKIVEGQRYS 528

Query: 441  GKLSDEQTARMLKMGCQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRV 500
             +L++ Q   +LK+ CQRP +R+ +I   +Q           +EF +++S  +  +  R+
Sbjct: 529  KRLNERQITALLKVTCQRPIDREKDILQTVQ-LNDYAKDNYAQEFGIKISTSLASVEARI 588

Query: 501  LQPPKLKLGDGGQIRDLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRF 560
            L PP LK  + G+    +P     QWN+++  +  G  +  W  I+F       L    F
Sbjct: 589  LPPPWLKYHESGREGTCLP--QVGQWNMMNKKMINGGTVNNWICINFSRQVQDNL-ARTF 648

Query: 561  INQLSRRCEQLGIYLNSKTVVPP-QFEPTQVLNSVSLLESKLK-KLHGAASNNLQ----- 620
              +L++ C   G+  N + V+PP    P QV       E  LK + H A S   Q     
Sbjct: 649  CQELAQMCYVSGMAFNPEPVLPPVSARPEQV-------EKVLKTRYHDATSKLSQGKEID 708

Query: 621  -LLICVMERKHKGYADLKRIAETSIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCT 680
             L++ + +     Y DLKRI ET +G+VSQCCL  ++ K+S Q++AN+ALKIN KVGG  
Sbjct: 709  LLIVILPDNNGSLYGDLKRICETELGIVSQCCLTKHVFKMSKQYMANVALKINVKVGGRN 768

Query: 681  VALYNSLPSQVPRLLRTDKPVIFMGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRM 740
              L ++L  ++P  L +D+P I  GADVTHPHP +D SPS+AAVV S +WP   KY   +
Sbjct: 769  TVLVDALSRRIP--LVSDRPTIIFGADVTHPHPGEDSSPSIAAVVASQDWPEITKYAGLV 828

Query: 741  RSQTHRQEIIVDL-------------GAMVEELLEEFYQEVNELPSRIIFFRDGVSETQF 800
             +Q HRQE+I DL             G M++ELL  F +     P RIIF+RDGVSE QF
Sbjct: 829  CAQAHRQELIQDLFKEWKDPQKGVVTGGMIKELLIAFRRSTGHKPLRIIFYRDGVSEGQF 888

Query: 801  YKVLQEELQAIKTACSRFH-NYKPPITFAVVQKRHHTRLFPFKVDPSSNHNE--VLDE-- 860
            Y+VL  EL AI+ AC+     Y+PP+TF VVQKRHHTRLF      + NHN+   +D   
Sbjct: 889  YQVLLYELDAIRKACASLEAGYQPPVTFVVVQKRHHTRLF------AQNHNDRHSVDRSG 948

Query: 861  NIPPGTVVDTVITHPKEFDFYLCSHWGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCY 920
            NI PGTVVD+ I HP EFDFYLCSH G++GTSRP HYHVL DEN FT+D LQ L  NLCY
Sbjct: 949  NILPGTVVDSKICHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQSLTNNLCY 1008

Query: 921  TYNRCTKPVSLVPPAYYAHLAAYRGRLYLE---------RSDSTAHTRGVSTVSRAAP-- 952
            TY RCT+ VS+VPPAYYAHLAA+R R Y+E          S S A   G++  S   P  
Sbjct: 1009 TYARCTRSVSIVPPAYYAHLAAFRARFYMEPETSDSGSMASGSMARGGGMAGRSTRGPNV 1050

BLAST of Spg025899 vs. TAIR 10
Match: AT2G27880.1 (Argonaute family protein )

HSP 1 Score: 600.1 bits (1546), Expect = 3.0e-171
Identity = 366/944 (38.77%), Postives = 535/944 (56.67%), Query Frame = 0

Query: 53  PILKISPAPKGLRLKSSLPLKGENGKVS-----------ESKPEV----------LVAAR 112
           P   ++ + K + + SS   + +N +VS           E+KPE+          +    
Sbjct: 90  PTFSVASSSKTVSVASSSKEESKNTEVSETMSNLQITSTETKPEMTSLPPASSKAVTFPV 149

Query: 113 RPDSGGVEGPVIPLLANHFLVQFDPSQRIYHYNVEISPNP-SKEVARMIKQKLVEDNSDL 172
           RP  G + G  + + ANHFLVQ    + +YHY+V I+P   SK V R + + LV++  D 
Sbjct: 150 RPGRGTL-GKKVMVRANHFLVQV-ADRDLYHYDVSINPEVISKTVNRNVMKLLVKNYKDS 209

Query: 173 -LSGASPAYDGRKNLYSPIEFVRDRLEFYVSLPIPSSKMKVSEGEIKHKQEFKLFRISIK 232
            L G SPAYDGRK+LY+      D  EF V+L       K ++G     + FK   +++K
Sbjct: 210 HLGGKSPAYDGRKSLYTAGPLPFDSKEFVVNL-----AEKRADGSSGKDRPFK---VAVK 269

Query: 233 LVSKFDGMELNRYLTKEGDDWIPIPQDYLHALDVVLREGPNENCFPVGRSLYSSSMW--- 292
            V+  D  +L ++L ++  +    P D +  LDVVLR+ P+ +   VGRS + +S+    
Sbjct: 270 NVTSTDLYQLQQFLDRKQRE---APYDTIQVLDVVLRDKPSNDYVSVGRSFFHTSLGKDA 329

Query: 293 --GSKEIGGGVVGLRGFFQSLRPTQQGLAMNVDCSVTAFHESIGVIPYLQKRLDFLRDLS 352
             G  E+G G+   RG+FQSLR TQ GL++N+D S  +F+E I V  ++ K L+ +RDL+
Sbjct: 330 RDGRGELGDGIEYWRGYFQSLRLTQMGLSLNIDVSARSFYEPIVVTDFISKFLN-IRDLN 389

Query: 353 QRKTRGLTCEERREVEKALKHIRVFVCHRESVQRYRVYGLTDEPTESLWFADRNGKNLRL 412
               R L   +R +V+K L+ ++V + H    +  ++ G++  P   L F   +     +
Sbjct: 390 ----RPLRDSDRLKVKKVLRTLKVKLLHWNGTKSAKISGISSLPIRELRFTLEDKSEKTV 449

Query: 413 VGYFKDHYNYDIQYRNLPCLQI-SRSKPCYLPMELCMICEGQKFLGKLSDEQTARMLKMG 472
           V YF + YNY ++Y+ LP +Q  S ++P YLPMELC I EGQ++  +L+++Q   +LK  
Sbjct: 450 VQYFAEKYNYRVKYQALPAIQTGSDTRPVYLPMELCQIDEGQRYTKRLNEKQVTALLKAT 509

Query: 473 CQRPKERKANIDGVMQGPVGPTSGVQGREFNLQVSREMTKLSGRVLQPPKLKLGDGGQIR 532
           CQRP +R+ +I  ++       +    +EF + V+ ++  +  RVL PP LK  D G+ +
Sbjct: 510 CQRPPDRENSIKNLV--VKNNYNDDLSKEFGMSVTTQLASIEARVLPPPMLKYHDSGKEK 569

Query: 533 DLIPSRHDRQWNLVDCHVFEGTRIERWSLISFGGSPDQRLNIPRFINQLSRRCEQLGIYL 592
            + P     QWN++D  +  G ++  W+ +SF    D+ L    F  QL   C   G+  
Sbjct: 570 MVNPRL--GQWNMIDKKMVNGAKVTSWTCVSFSTRIDRGLP-QEFCKQLIGMCVSKGMEF 629

Query: 593 NSKTVVPPQFEPTQVLNSVSLLESKLKKLHGAASNNLQLLICVMERKHKGYADLKRIAET 652
             +  +P    P +       +E  L  +H  A   LQLLI ++      Y  +KRI ET
Sbjct: 630 KPQPAIPFISCPPE------HIEEALLDIHKRAP-GLQLLIVILPDVTGSYGKIKRICET 689

Query: 653 SIGVVSQCCLYPNLAKLSSQFLANLALKINAKVGGCTVALYNSLPSQVPRLLRTDKPVIF 712
            +G+VSQCC    + KL+ Q++ N+ALKIN K GG    L +++   +P  L TD+P I 
Sbjct: 690 ELGIVSQCCQPRQVNKLNKQYMENVALKINVKTGGRNTVLNDAIRRNIP--LITDRPTII 749

Query: 713 MGADVTHPHPLDDFSPSVAAVVGSMNWPAANKYVSRMRSQTHRQEIIVDLGAMVEE---- 772
           MGADVTHP P +D SPS+AAVV SM+WP  NKY   + +Q HR+EII DL  +V++    
Sbjct: 750 MGADVTHPQPGEDSSPSIAAVVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQDPQRG 809

Query: 773 -----LLEE----FYQEVNELPSRIIFFRDGVSETQFYKVLQEELQAIKTACSRF-HNYK 832
                L+ E    F +   ++P RIIF+RDGVSE QF +VL  E+ AI+ AC+    NY 
Sbjct: 810 LVHSGLIREHFIAFRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQENYV 869

Query: 833 PPITFAVVQKRHHTRLFPFKVDPSSNHNEVLDE--NIPPGTVVDTVITHPKEFDFYLCSH 892
           P +TF +VQKRHHTRLFP        + ++ D+  NI PGTVVDT I HP EFDFYL SH
Sbjct: 870 PRVTFVIVQKRHHTRLFP----EQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNSH 929

Query: 893 WGVKGTSRPTHYHVLCDENQFTSDELQKLVYNLCYTYNRCTKPVSLVPPAYYAHLAAYRG 952
            G++GTSRP HYHVL DEN FT+D+LQ L  NLCYTY RCTK VS+VPPAYYAHLAA+R 
Sbjct: 930 AGIQGTSRPAHYHVLLDENGFTADQLQMLTNNLCYTYARCTKSVSIVPPAYYAHLAAFRA 989