Spg025846 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025846
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description1,4-alpha-D-glucan glucanohydrolase
Locationscaffold7: 2205544 .. 2213232 (+)
RNA-Seq ExpressionSpg025846
SyntenySpg025846
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAATTTGAGAACCTAACCATGCAGAATTTCACTGGAAATGAATCTAATTGAATTCGACAGATATTTTATTCCTAACGCAAATCCGGAAATCAAAAAGGTTCACATCTTTAAATTGCATTTTAATTAATCAAATATCGTCCACTCTTGTCATTTATCTCCAATCTCTCTCCTTTTCCTTTTCCTTTTCTCTGTTCTGTTCTTCGTTCAATTTATATCTTGAATCTGCGCTGACGAGGGAGTGGCAACAGTGGGATCATGTCGACGATTGGAGTCGATCCTCTTCTCTATCACTGTGGTAAAGGGAAGCACAGATTCCACCACCGTACTCGATTCAATACGCTCAAACCCTGTTCCTTTAGTTACTGTCGCAATAAGCTTCTTTACCGTGGCCGAAGGAGCTTTCTCCATCACAATTCTTATAGGCTTCCTGTTCTTAGAGCTAGCTCACCTAATGCGGCTCTTCTTGAAGCTTTCCAATCTACAGATGTTCTGCTTAAGGAGACTTTTCCTCTCAAGCGCACCGAAAAGGTTTGAATTATATTTATTTCTTTTTCTTCTTACTGTTTAATTTGTGGGATCTGTTTTATTGCTGCTGGGAAGTGCACTTCCTTTTTGCTTCGTTGAGTTATTCACTCTCAACGACGAACTAAATTCAACCATCCCCAATTCTTATTGTTTAATTTGTGGGATCTGTTTTATTGCTGCTGGGAAGTGCACGTCCTTTTTGCTTCGTTGAGTTGTTCACTCTCGAGATGGGAATTTTTTGTTTCCCTTTGCTCGTTTAACTTGTTTTGCATAAGAATTGGGGATGGTTGAATTTAGTTCGTCGTTGTTGATTTGAGCTTGTAGTTGGAGGGAAGGATCTCTGTCAGGCTAGCCCGGGGGAAGGACCAGAGTCAATGGGAGCTTACTGTAGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAGTCGATGATATTGGCAGGTATTTCTCGTTTTAATCTTGGTTCCATTGGATATGATAAGAAATTAACATGATTTTAAAAATTGCTGATGCTGAATTTGTTTCGTTGAGATACCTTGCCTTCATCTAAAATGGGTTGAATGAATGAACGTTGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCTCCGGGTTCAATACCCATCAAAGTATGTTACTTTAATCTCTGAACTTCAATGTCTAAGCTGCTCTTGTATTGCCTGCAAAAGTGTAATAACAAGTATATTTGCAGGATTATGCTATTGAGACACCATTGAAGAAACCATCCTCGTCTTCTTTGGGAGATGTTCATGAAGTGAAGATAGAAATTGTTCCTGACAAAACAATTGCAGCTATAAATTTTGTTTTGAAGGTTCTTATTTTCAGTATTTAGCAATTGAGGACGTGTGCTATTCCTCTTTGATCCTTGATCATGCGCTCGTTTCTAATATTGCAAGTCCTTTTGGTGAATGGGACTTCTTATTTTTGGTAGTGCAGGATGAAGAAACTGGAAGTTGGTATCAGCACAAAGGGAGAGATTTTAGGGTCCCTCTTTTAGACTACCATGGTGATGATGGCAATATAGTTGGATCGATTAAGGGCTTGGGTTTATGGCCAGGTTGGAGAGGTCCTTTTTTTTTCTTTTTTCTTTTTTTTTTTTTTGTTGTTGTTGTAAGGTTGGAGAGGTCCTTGAGTGATATATAATGTTTCTTTGAATTTGGCTTTGCATGATATTCTCTTTACTTTTTGTTTCTCCTTTCTATTTATTCATACTTTTCCATCTATTATTGTAGTTTGTTTTCGTACATAGCCCCTACTGAATTATTTTTTACATAAAAAATTAAAATAAAATAAACCTGAAATCGTCTTGATTGGAAGCATTCATAGAACTGAATAAGACCGATAGAGATAAATGATTTTATGTTCATTGTGTCGTTCAATTGACGGTGCAATAAACCACGGATAAAGTTTTTTTCTCTTTTTCTCTTTAATCTCTCTTCTGTCCTGCTCAATTATATCTGAATGCATTGTAGGAGTTGTAGTTTTAGCTGTTGGACAGCAAGACATACTAAGTGATTTCCTTCTCTGTATACATTCTTAGAGCATCAAACTAGAAAGTAGTGCTGATAGTCTTTTTGCATTTGTGACATAGGTACATTCCTTGCTTCCATTGTGCACTAGAGGGAATTTAATTTAGTTATAATTTAAAATTGCCTGCCTCACACCCTGTTTCCTTGGTGTTTATCTCATTCTTTCAGCTATTGTCTATCTCATGTGGGTGGGATGTACTTTTTTAATTTCTTCAATTGTAGGGGCTTTGGGTCAGCTATCCAACCTCCTTGTCAAACCAGAATCAAATACTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGGAGAAGAAGTCTTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTAGCTGCTGATAATTCGGTCAGTGTTTCAGTTAGAAAGTGCCCCGAAACAACCAAATATCTTTTGTATTTGGAAACTGATCTACCTGGAGATGTTATTGTTCATTGGGGAGCGTGCAGAAATGAGACTAGGAAATGGGAGATGCCAGCTGCTCCTCATCCTCCCGAAACAACAGTATTCAAGAACAAGGCTTTGCGTACCCTACTGCAGGTTTTGTGCAACATTCTTTTTCCCTGAAATCTGAATCAATACTAATTGCAAACTCATCAAATGTCTCAATTTTTTGTTATGGACACAAAATTATTATTATTAAGCTATCTAAAAACCGTTGCATAGTCTTCCATTTTGTGAGAACTCTTTTCTAGTATTATTGGGACAACAAATAGAAATTTTCAGGCATATACTTGGAGGGAACCTTGAGTGCGGTCTCCTTTAAGTTCTTAAGCTATGTGGTAAAAAATGATAGTAATTGATCTATCGTTCATTAAAAGGTTTTTTTTTTTTTTTTTTTTTTTTAATAAAATCTGCCGCATCTTTAACTATGTATGAATAAGCCTTCTGGATGATAAAAATAAAAATTGACTACATTTTTTGTATAGAAATAAGAAATATTAAGTACATCTTGGAAAATTCTATTCAAGCGTATCTTTTAAAATTCATCAGAGTGATCCATATTTTATACATTCAATACACACATTTGCTTTTCAAATTCATGTTTGGATTTTGTAAAATAGCTTAAGTTTTTTAGTCAAGAATATTGTACTTTTGATTAATTATAAAATGTCCTGTTTGCAGCCAAAAGAAGGGGGAAAAGGATGTTCTGGAATTTTTACCATAGAAGAAGAATTTGGAGGATTTCTTTTTGTTCTAAAGCAGAATGAGAACTCTTGGTTAAACTACAAGGGAGATGACTTCTACATCCCCTTCTCAAGTTTTGGTAACCTGCCTGATCAACAGAGCCAGAGTGAAATAGAAGATACAGTAGTATCTAGAAAATCTGGAGAAGAATCTGAAGGAGTTTCTGTTACTGCTTATACCGATGGAATTATCAAAGAAATAAGAAATTTGGTTACTGACATTTCTTCTCAGAAGTCACCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAATTGGCTGCCGAAGCCTATAGTATCTTCAGAAGTTCTGCTCCAACTTTTACAGAAGAAATAATTGAGACGCCCAAACCTGTTGAACCACCCGTGCCAATATCCTCCGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAATTGGGAATCTCACAAGTCTGGAAGATGGTACATGGAACTTAAAGAGAAAGCTGCAGAATTATCCTCCCTTGGTTTCACTGTGCTATGGTTGCCACCTCCAACTGAATCTGTGTCACCAGAAGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGGTACATTTATAAAAAGATTATAGTTTATATGACATGATAATTTTGATATAGATTTCTATGATATTGCTTCATACGGGGAACAGGTAGAACCCCTTTTGTTTCAATCCACTCCTAGATTAAAACATTATCACTTGTCAGTAGCAGTTATCATTCTATGATCCTTCAAAATTGTAATTCTATCTCATGCATGGAATCTAGAGAAATGTGCTCTAGTGATCTGAGGACAACCCCTTCAAAAACTTTTGATTTACTCTTTCCCGTCATATTTAGTGAATTGAAAAATAGAAGACATCATCATTACAAGGATATTTGTCAACACTCCACTTTGTTCATTTCAGATATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGAGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTCTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAGTAGTTTCAGATGATCCACATTTTCAGGTTTGATTTTTCTTTTTGGCTTCTCAGATGCCTGCAGTTTAGATTCTATTTAATTTTCCACTTATATTTTATGAAATATTGCATGATGAAATGAAAATAAAAAGTATGACACCATACCAGTAATGGTGGCTGGGTTCATCTCTTTTTACATTTTATTGGTCACTGAATCATTTGTTTCCTCCTTAAAGAAAAGTAATACACTATGGAACTATTAAAAATAAAAAATACTTTTTGAAAAATTAGATCTTTGATATGAATGATTGTCCACTCTCAACTTATATGATCTAAAATTAATTATCTTAAATTTAGGTTGTGGAATAAGAAAATTTCTCATAAAGTAAACTATAGCGACTATCAAAACATCAAATTGAATGGGATTGTTTATGATTTCTGAAAATTTTGACGAATGCTTTGCATGCAGAAGAAAAATACTTCATATCCCCATTATGTTATAAAATTTTGACAACAGGCAGAAGAAAAATGGCACGAGATATACCCTGTGCAGATGTGCAAATACTATCATTGAACTTTTTAAATATATTGCCTCGTTTATATAACATAAGATGTTGACTTCATTTAAATAGTTACATTTTCTTTATTATTTTTGGTTACTCGGACAACAAAATTGATTATTCATTTTTCATGTCATGTAAAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCATGCTGCGCCTAATATTGATCATTCACAAGAGTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGGTTAATTCCCCAATCAACCCTTTTTTTGGCTGAACTATTAATTTCTGTGGAACTTCTTTTTTGTTTTTTTTGTCTTCTATATGAAGACCTTATCGTATAAAAAACAAACGTTAAGTAGTGTTTATAAAGTAGTATGATATGATAACCCAAAATATTGACATAGCGATGAGAAGAGGCTTTGGACTCAACCAAGAGGTCTTACTCTCTCATTGCATAGGCTGCTATTTGAGATGTATACCCTTGAGACCGTTTTTTTTATGCTAAGTTGTATGATTAGATATAATTCCTCTCTTTGAACTTGTTGAACATAAAAATTTAAGGGAATTTCATAATAAAATGGTTAATTAACCAAATATGTGAAGGGTTTGAATTAAGTTGGTTCATGAACGTTTCCTGGTCTCATTAATTCTTAGAAACGATCAGAATATTAGCCACCGTGTTAGGGTGTGGATATGCTTTATTTTCTTTTCAATCTTTTTAATTGGTGGGTTTCATTCTGGTTGTTTAAACGAAGTGCTTGATGTTCTTAACTTCTATGATTTGCTGACATGAATATGCACATTACTTTCCTAATCAACTCGAATGTCTCTTTGGCACATTTCTTTTAAAAATTAGAGGAGCATTGCCTTTATGCGTATGTTTCTGGTTGTTGACATGCAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGCTTCTGGGGTGGTTATGTGAAGGATTACTTGGAAGCAAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATTAATGCTACTAATGGAACTGCTGGGGCATTTGATGTTACTACCAAAGGAATTCTTCATTCTGTAAGCTTTCATTTAGTTATCTGGTTGTCAAATCTGTGGAAGATGTTTTCTGAGTTTATTCACAATATAACTTATATTATACCCACAAGAAAAGGAATTGTTATTATATTGTCATAATATCTGCCTTCTAATTGTATCTGTTTTAGATGTGTCTGACGGATTCAATTATAAAAAATCATATTTTTGTTGGTTTGATGCAGTAGTTCTGCAGCAATCCCTAAGATTCTTGATGCTGCAAGAAGTTTTTCTTGCTACTCGTAGAACGTTATATAACCATTTGGAAAATATCTCTTATAGGCACTTGAAAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCTGGAGTTGTTGGATGGTGGCCCTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGTTTAAAATTCTTTTATAAGAACTAATTTATGGCATTTAAAACTGAAGTCGGCTGCGTGAAACATTCATTAGAGATGCACTCTGATGTTTTACTCTTGGAATTGTTAATATCATTAAACTGATAATCATATCAATTCCTATGATTTTCTGTATATTTCTTTATGCTATGAAGAATCTACAATTTTCATGGTATCATCAACTCGTGTGTTACCTTGTGATGCTGTTTTCTGATTTGTAGGGTCATTGGAGATTTCCTGGTGGGAAGGAAATGCAAGGATATGCTTACATCCTGACTCATCCAGGGACGCCATCAGTATTCTTTGACCACGTTTTCTCCCACTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAATCAACTGCCGGAGTGTAGTAAGTATAAATGACCATTTATTTTCTTTTCGTTATTGAAAATTTGAAGAAATTGACACTATTAACTCTTCCTACTCCTACTCCTAGGAGTGATTCTAGAATGAACTTTTGTCATGTCCAAAATCACTAAAAAGAAGTCTGACAAAATTGATTTTGGCCATTCTAAAATCACTTTCAAACGTGTCTTTAATTTCTTAGTCTCAAGACCATGAAATCGCCTATGCCTTCACAGATCAAGAACAAATATAACTATGCTTTAACTACTTTTACTTCATTTCTCAGGTTAAAATTGAGAAGGCGGAAAGGGATTTCTATGCAGCCACCATTGATGAAAAAGTTACAGTGAAGATTGGACCAGGCCATTACGAACCTCCTAACGGATCGAAGGGATGGTCATTAGTGATCGAGGGAAAAGACTACAAGGTCTGGGAAGCATCCAAGTAAATTCTGATGAATCAGGAAATTTAGACAATAACCAACTTCTATGAGACTTTGTGCTTGTTGGATGAACCTCATGTAAGTTATACCAGAAGGCTGAAGATAATGCTGCTATTGGATTGTGGATAGGAGATTTATACTGTTTTATATGTATCATACATATTCATTGGCTCTAAGAGAAGTATTCATTGAGGATGCTGTTAATGTTATTGTAAAGTTTCACCAATTGGTGACTATGTATAAACTTATCAGCAGCTTAATAAATATACAAAGGAGATGTGGGTGATTCAATTGTACCCACCCTTTTCCCA

mRNA sequence

ATGTCGACGATTGGAGTCGATCCTCTTCTCTATCACTGTGGTAAAGGGAAGCACAGATTCCACCACCGTACTCGATTCAATACGCTCAAACCCTGTTCCTTTAGTTACTGTCGCAATAAGCTTCTTTACCGTGGCCGAAGGAGCTTTCTCCATCACAATTCTTATAGGCTTCCTGTTCTTAGAGCTAGCTCACCTAATGCGGCTCTTCTTGAAGCTTTCCAATCTACAGATGTTCTGCTTAAGGAGACTTTTCCTCTCAAGCGCACCGAAAAGTTGGAGGGAAGGATCTCTGTCAGGCTAGCCCGGGGGAAGGACCAGAGTCAATGGGAGCTTACTGTAGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAGTCGATGATATTGGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCTCCGGGTTCAATACCCATCAAAGATTATGCTATTGAGACACCATTGAAGAAACCATCCTCGTCTTCTTTGGGAGATGTTCATGAAGTGAAGATAGAAATTGTTCCTGACAAAACAATTGCAGCTATAAATTTTGTTTTGAAGGATGAAGAAACTGGAAGTTGGTATCAGCACAAAGGGAGAGATTTTAGGGTCCCTCTTTTAGACTACCATGGTGATGATGGCAATATAGTTGGATCGATTAAGGGCTTGGGTTTATGGCCAGGAGTTGTAGTTTTAGCTGTTGGACAGCAAGACATACTAACTATTGTCTATCTCATGTGGGTGGGATGTACTTTTTTAATTTCTTCAATTGTAGGGGCTTTGGGTCAGCTATCCAACCTCCTTGTCAAACCAGAATCAAATACTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGGAGAAGAAGTCTTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTAGCTGCTGATAATTCGGTCAGTGTTTCAGTTAGAAAGTGCCCCGAAACAACCAAATATCTTTTGTATTTGGAAACTGATCTACCTGGAGATGTTATTGTTCATTGGGGAGCGTGCAGAAATGAGACTAGGAAATGGGAGATGCCAGCTGCTCCTCATCCTCCCGAAACAACAGTATTCAAGAACAAGGCTTTGCGTACCCTACTGCAGCCAAAAGAAGGGGGAAAAGGATGTTCTGGAATTTTTACCATAGAAGAAGAATTTGGAGGATTTCTTTTTGTTCTAAAGCAGAATGAGAACTCTTGGTTAAACTACAAGGGAGATGACTTCTACATCCCCTTCTCAAGTTTTGGTAACCTGCCTGATCAACAGAGCCAGAGTGAAATAGAAGATACAGTAGTATCTAGAAAATCTGGAGAAGAATCTGAAGGAGTTTCTGTTACTGCTTATACCGATGGAATTATCAAAGAAATAAGAAATTTGGTTACTGACATTTCTTCTCAGAAGTCACCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAATTGGCTGCCGAAGCCTATAGTATCTTCAGAAGTTCTGCTCCAACTTTTACAGAAGAAATAATTGAGACGCCCAAACCTGTTGAACCACCCGTGCCAATATCCTCCGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAATTGGGAATCTCACAAGTCTGGAAGATGGTACATGGAACTTAAAGAGAAAGCTGCAGAATTATCCTCCCTTGGTTTCACTGTGCTATGGTTGCCACCTCCAACTGAATCTGTGTCACCAGAAGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGATATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGAGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTCTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAGTAGTTTCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCATGCTGCGCCTAATATTGATCATTCACAAGAGTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGCTTCTGGGGTGGTTATGTGAAGGATTACTTGGAAGCAAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATTAATGCTACTAATGGAACTGCTGGGGCATTTGATGTTACTACCAAAGGAATTCTTCATTCTGCACTTGAAAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCTGGAGTTGTTGGATGGTGGCCCTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGGTCATTGGAGATTTCCTGGTGGGAAGGAAATGCAAGGATATGCTTACATCCTGACTCATCCAGGGACGCCATCAGTATTCTTTGACCACGTTTTCTCCCACTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAATCAACTGCCGGAGTGTAGTTAAAATTGAGAAGGCGGAAAGGGATTTCTATGCAGCCACCATTGATGAAAAAGTTACAGTGAAGATTGGACCAGGCCATTACGAACCTCCTAACGGATCGAAGGGATGGTCATTAGTGATCGAGGGAAAAGACTACAAGGTCTGGGAAGCATCCAAGTAA

Coding sequence (CDS)

ATGTCGACGATTGGAGTCGATCCTCTTCTCTATCACTGTGGTAAAGGGAAGCACAGATTCCACCACCGTACTCGATTCAATACGCTCAAACCCTGTTCCTTTAGTTACTGTCGCAATAAGCTTCTTTACCGTGGCCGAAGGAGCTTTCTCCATCACAATTCTTATAGGCTTCCTGTTCTTAGAGCTAGCTCACCTAATGCGGCTCTTCTTGAAGCTTTCCAATCTACAGATGTTCTGCTTAAGGAGACTTTTCCTCTCAAGCGCACCGAAAAGTTGGAGGGAAGGATCTCTGTCAGGCTAGCCCGGGGGAAGGACCAGAGTCAATGGGAGCTTACTGTAGGATGTAACCTTTCTGGGAAATGGATTCTTCATTGGGGTGTGTCCTTAGTCGATGATATTGGCAGCGAATGGGATCAACCTCCTAAAGAAATGATACCTCCGGGTTCAATACCCATCAAAGATTATGCTATTGAGACACCATTGAAGAAACCATCCTCGTCTTCTTTGGGAGATGTTCATGAAGTGAAGATAGAAATTGTTCCTGACAAAACAATTGCAGCTATAAATTTTGTTTTGAAGGATGAAGAAACTGGAAGTTGGTATCAGCACAAAGGGAGAGATTTTAGGGTCCCTCTTTTAGACTACCATGGTGATGATGGCAATATAGTTGGATCGATTAAGGGCTTGGGTTTATGGCCAGGAGTTGTAGTTTTAGCTGTTGGACAGCAAGACATACTAACTATTGTCTATCTCATGTGGGTGGGATGTACTTTTTTAATTTCTTCAATTGTAGGGGCTTTGGGTCAGCTATCCAACCTCCTTGTCAAACCAGAATCAAATACTAAAGGTCAAGAAAGCAGTGGTGAATCTGGAGATACAAAGGAGGAGAAGAAGTCTTTGGAAGGATTCTACGAAGAGCTGCCTATTGTAAAAGAAGTAGCTGCTGATAATTCGGTCAGTGTTTCAGTTAGAAAGTGCCCCGAAACAACCAAATATCTTTTGTATTTGGAAACTGATCTACCTGGAGATGTTATTGTTCATTGGGGAGCGTGCAGAAATGAGACTAGGAAATGGGAGATGCCAGCTGCTCCTCATCCTCCCGAAACAACAGTATTCAAGAACAAGGCTTTGCGTACCCTACTGCAGCCAAAAGAAGGGGGAAAAGGATGTTCTGGAATTTTTACCATAGAAGAAGAATTTGGAGGATTTCTTTTTGTTCTAAAGCAGAATGAGAACTCTTGGTTAAACTACAAGGGAGATGACTTCTACATCCCCTTCTCAAGTTTTGGTAACCTGCCTGATCAACAGAGCCAGAGTGAAATAGAAGATACAGTAGTATCTAGAAAATCTGGAGAAGAATCTGAAGGAGTTTCTGTTACTGCTTATACCGATGGAATTATCAAAGAAATAAGAAATTTGGTTACTGACATTTCTTCTCAGAAGTCACCAAAGAAAAAAACCAAAGAAGCACAAGAAAGCATTCTTCAGGAAATTGAAAAATTGGCTGCCGAAGCCTATAGTATCTTCAGAAGTTCTGCTCCAACTTTTACAGAAGAAATAATTGAGACGCCCAAACCTGTTGAACCACCCGTGCCAATATCCTCCGGCACAGGTTCAGGTTTTGAAATATTGTGCCAAGGGTTTAATTGGGAATCTCACAAGTCTGGAAGATGGTACATGGAACTTAAAGAGAAAGCTGCAGAATTATCCTCCCTTGGTTTCACTGTGCTATGGTTGCCACCTCCAACTGAATCTGTGTCACCAGAAGGGTACATGCCAAAGGACTTGTATAATTTAAATTCCAGATATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGAGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTCTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGATCGTGCAGTAGTTTCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGATAACTTCCATGCTGCGCCTAATATTGATCATTCACAAGAGTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTATGATGGGTGGAGGCTTGATTTTGTCAGAGGCTTCTGGGGTGGTTATGTGAAGGATTACTTGGAAGCAAGTGAACCTTACTTTGCTGTAGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGAGATGGATCATAATCAAGATGCTCATAGACAGAGAATTGTTGACTGGATTAATGCTACTAATGGAACTGCTGGGGCATTTGATGTTACTACCAAAGGAATTCTTCATTCTGCACTTGAAAGATGTGAATATTGGCGATTATCAGATCAGAAAGGAAAGCCTCCTGGAGTTGTTGGATGGTGGCCCTCTCGTGCTGTTACCTTTATAGAGAATCATGACACTGGATCTACTCAGGGTCATTGGAGATTTCCTGGTGGGAAGGAAATGCAAGGATATGCTTACATCCTGACTCATCCAGGGACGCCATCAGTATTCTTTGACCACGTTTTCTCCCACTACAAATCTGAAATTGCAGCACTTATTACTCTCAGACAACGAAATAAAATCAACTGCCGGAGTGTAGTTAAAATTGAGAAGGCGGAAAGGGATTTCTATGCAGCCACCATTGATGAAAAAGTTACAGTGAAGATTGGACCAGGCCATTACGAACCTCCTAACGGATCGAAGGGATGGTCATTAGTGATCGAGGGAAAAGACTACAAGGTCTGGGAAGCATCCAAGTAA

Protein sequence

MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVLRASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGKWILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIVPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLAVGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKSLEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKGDDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK
Homology
BLAST of Spg025846 vs. NCBI nr
Match: XP_038891960.1 (alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1693.7 bits (4385), Expect = 0.0e+00
Identity = 810/935 (86.63%), Postives = 855/935 (91.44%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MSTI +DPLLYHCGKGKHRFH+R RFNTLKPCSFSYC NKLL+ GR+SF+++NSYRLP  
Sbjct: 1   MSTISLDPLLYHCGKGKHRFHYRPRFNTLKPCSFSYCPNKLLHHGRKSFVNYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           RAS+ NA LL+ FQS DVL +ETFPLKR EKLEGRISVRLA+G DQ+QWELTVGCNL+GK
Sbjct: 61  RASATNAPLLQTFQSKDVLFQETFPLKRNEKLEGRISVRLAQGNDQNQWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSIPIKDYAIETPLKK SSSS GD VHEVKI +
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSSGDFVHEVKINL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
           VPDKTIAAINFVLKDE+TG WYQHKGRDF+VPLLDYHG+DGNIVG+ KGLGLWP      
Sbjct: 181 VPDKTIAAINFVLKDEQTGIWYQHKGRDFKVPLLDYHGEDGNIVGTKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK ES++K +  SGESGD KEEKK 
Sbjct: 241 -------------------------GALGQLSNLLVKAESSSKDKGRSGESGDAKEEKKY 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNSVSVSVRKCPETTK LLYLETDLPGDVIVHWGACR+ETRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSVSVSVRKCPETTKNLLYLETDLPGDVIVHWGACRDETRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGC GIFTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCCGIFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP S+ GNLP QQ +SE++D  VS+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLSNSGNLPGQQRESELKDAGVSKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEE+IE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEVIEMPKPVEPPVQISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKE+AAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKERAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGDVVLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPCGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AYILTHPGTPS F+DH+FSHYKSEIAALI+LR+RNK+NCRSVV IEKAERD YAA IDEK
Sbjct: 841 AYILTHPGTPSAFYDHIFSHYKSEIAALISLRKRNKVNCRSVVTIEKAERDVYAAIIDEK 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 904

BLAST of Spg025846 vs. NCBI nr
Match: KAA0034391.1 (alpha-amylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 798/935 (85.35%), Postives = 852/935 (91.12%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+++D+  S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901

BLAST of Spg025846 vs. NCBI nr
Match: XP_008446314.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo])

HSP 1 Score: 1668.7 bits (4320), Expect = 0.0e+00
Identity = 796/935 (85.13%), Postives = 848/935 (90.70%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+ +     +KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKXKTAEHLKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901

BLAST of Spg025846 vs. NCBI nr
Match: XP_004135194.1 (alpha-amylase 3, chloroplastic [Cucumis sativus] >KGN51888.1 hypothetical protein Csa_008336 [Cucumis sativus])

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 794/934 (85.01%), Postives = 846/934 (90.58%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC KGKHRFHHR RFN L+PCSF+YC NKLL  GR+SF+H+NSYR P +
Sbjct: 1   MSSIALDPLLYHCAKGKHRFHHRPRFNMLRPCSFTYCPNKLLCHGRKSFVHYNSYRPPTI 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL  ETFPLKR EKLEGRISVRLA+GKD + WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFNETFPLKRNEKLEGRISVRLAQGKDHNNWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIV 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GDVHEVKI++ 
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVHEVKIDLA 180

Query: 181 PDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLAV 240
           PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGN VG+ KGLGLWP       
Sbjct: 181 PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNKVGTKKGLGLWP------- 240

Query: 241 GQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKSL 300
                                   GALGQLSNLLVK E+N+K Q SS ESGDTKEEKKSL
Sbjct: 241 ------------------------GALGQLSNLLVKAETNSKDQGSSSESGDTKEEKKSL 300

Query: 301 EGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWEM 360
           EGFY+ELPIVKE+A DNS+SVSVRKC ETTKYLLYLE+DLPGDVIVHWGACR++T+KWE+
Sbjct: 301 EGFYKELPIVKEIAVDNSISVSVRKCSETTKYLLYLESDLPGDVIVHWGACRDDTKKWEI 360

Query: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKGD 420
           PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQ ENSWLNYKGD
Sbjct: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQKENSWLNYKGD 420

Query: 421 DFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
           DFYIPF S GNL +QQ +S+++DT  S+ SGEESEGVSVTAYTDGIIKEIRNLVTDISSQ
Sbjct: 421 DFYIPFPSSGNLSNQQRKSKLKDTRASKISGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480

Query: 481 KSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGSG 540
           K+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV ISSGTGSG
Sbjct: 481 KTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVRISSGTGSG 540

Query: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
           FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN
Sbjct: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600

Query: 601 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVSD 660
           SRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVSD
Sbjct: 601 SRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSD 660

Query: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGG 720
           DPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGG
Sbjct: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLLWLRKEIGYDGWRLDFVRGFWGG 720

Query: 721 YVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
           YVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG
Sbjct: 721 YVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780

Query: 781 ILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
           ILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA
Sbjct: 781 ILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840

Query: 841 YILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEKV 900
           Y+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAA IDE V
Sbjct: 841 YLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAAIIDETV 900

Query: 901 TVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
            VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 AVKIGPGNFEPPSGSNGWSLVIEGKDYKVWEVSK 900

BLAST of Spg025846 vs. NCBI nr
Match: TYK15529.1 (alpha-amylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 793/929 (85.36%), Postives = 847/929 (91.17%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+++D+  S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 895

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYK 929
           V VKIGPG++EPP+GS GWSLVIEGKDYK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYK 895

BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 606/942 (64.33%), Postives = 722/942 (76.65%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFN----TLKPCSFS----YCRNKLLYRGRRSFLHH 60
           MST+ ++ LL+H         H ++ N    +  P S +    +  NKLL+   +S    
Sbjct: 1   MSTVPIESLLHH-----SYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60

Query: 61  NSYRLPV-LRASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWEL 120
           +  + PV +RA+S + A++E  QS DV+ KE FP++R EK EG+I VRL   K+++ WEL
Sbjct: 61  SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120

Query: 121 TVGCNLSGKWILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD 180
           +VGC++ GKWILHWGVS V D GSEWDQPP++M PPGSI IKDYAIETPLKK S      
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGD--S 180

Query: 181 VHEVKIEIVPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGL 240
             EV I +  + ++AA+NFVLKDEETG+WYQHKGRDF+VPL+D   D+GN++G+ KG   
Sbjct: 181 FFEVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF-- 240

Query: 241 WPGVVVLAVGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESG 300
                                            GALGQLSN+ +K       Q+ S    
Sbjct: 241 ---------------------------------GALGQLSNIPLK-------QDKSSAET 300

Query: 301 DTKEEKKSLEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGAC 360
           D+ EE+K L+ FYEE+PI K VA DNSVSV+ RKCPET+K ++ +ETDLPGDV VHWG C
Sbjct: 301 DSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVC 360

Query: 361 RNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNE 420
           +N T+KWE+P+ P+P ET++FKNKALRT LQ K+ G G  G+F+++ +  G  FVLK NE
Sbjct: 361 KNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNE 420

Query: 421 NSWLNYKGDDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIR 480
           N+WLNY+G+DFY+PF     L    S  E E   VS+   +  + VS + +T  II EIR
Sbjct: 421 NTWLNYRGEDFYVPF-----LTSSSSPVETEAAQVSKPKRKTDKEVSASGFTKEIITEIR 480

Query: 481 NLVTDISSQKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV 540
           NL  DISS K+ K   KE QE+ILQEIEKLAAEAYSIFRS+ P F+EE +   +  +P +
Sbjct: 481 NLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDI 540

Query: 541 PISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGY 600
            ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA EL+SLGFTVLWLPPPTESVSPEGY
Sbjct: 541 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 600

Query: 601 MPKDLYNLNSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLN 660
           MPKDLYNLNSRYG IDELKD VK FH V IKVLGD VLNHRCAH KNQNG+WN+FGGRLN
Sbjct: 601 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 660

Query: 661 WDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRL 720
           WDDRAVV+DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVR DIKEWLCW+ +E+GYDGWRL
Sbjct: 661 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 720

Query: 721 DFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTA 780
           DFVRGFWGGYVKDY++AS+PYFAVGEYWDSLSYTYGEMD+NQDAHRQRIVDWINAT+G A
Sbjct: 721 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 780

Query: 781 GAFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
           GAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP
Sbjct: 781 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840

Query: 841 GGKEMQGYAYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDF 900
            GKEMQGYAYILTHPGTP+VFFDH+FS Y SEIAAL++LR R K++CRS V I+K+ERD 
Sbjct: 841 EGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDV 887

Query: 901 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
           YAA IDEKV +KIGPGHYEPPNGS+ WS+ +EG+DYKVWE S
Sbjct: 901 YAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887

BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.7e-116
Identity = 193/395 (48.86%), Postives = 267/395 (67.59%), Query Frame = 0

Query: 540 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 599
           G E++ Q +NWESHK   W+  L  K  +++  GFT  WLPPP++S++PEGY+P+DLY+L
Sbjct: 24  GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83

Query: 600 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-RLNWDDRAVV 659
           NS YG+   LK  ++     +++ + D+V+NHR   ++   G++N + G  L WD+ AV 
Sbjct: 84  NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143

Query: 660 SDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 719
           S      G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+ 
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203

Query: 720 GGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 779
             YVK+Y+ A++P F+VGE WDS +Y    +D+NQD+HRQRI+ WI+AT   + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263

Query: 780 KGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 839
           KGIL  A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323

Query: 840 YAYILTHPGTPSVFFDHVF---SHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAAT 899
           YAYILTHPG P VF+DH +   S    +I  LI +R+R  I+ RS V++ KAE + YAA 
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383

Query: 900 IDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVW 931
           + EK+ +K+G G + P    + W+L   G  Y VW
Sbjct: 384 VGEKICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411

BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 5.8e-111
Identity = 202/402 (50.25%), Postives = 264/402 (65.67%), Query Frame = 0

Query: 543 ILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 602
           +L QGFNWES K G WY  LK    +L++ G T +WLPPP++SVSPEGY+P  LY+L+ S
Sbjct: 25  LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84

Query: 603 RYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-----RLNWDDRA 662
           +YG+ +ELK  +  FH+  IK L D+V+NHR A  K+  GI+ IF G     R +W    
Sbjct: 85  KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144

Query: 663 VVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVR 722
           +  DD  +  G GN  SG+ + AAP+IDH    V+ ++ EW+ WL+ EIG+DGWR DFV+
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204

Query: 723 GFWGGYVKDYLEASEPYFAVGEYWDSLSYTY-GEMDHNQDAHRQRIVDWINATNGTAGAF 782
           G+     K Y+E ++P FAVGE WDS+SY   G+ ++NQD+HR  +V+W+ +  G   AF
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAF 264

Query: 783 DVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGK 842
           D TTKGIL +A++  E WRL D  GKPPG++G  P  AVTFI+NHDTGSTQ  W FP  K
Sbjct: 265 DFTTKGILQAAVQG-ELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324

Query: 843 EMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDFYA 902
            MQGYAYILTHPGTPS+F+DH F    K +IA L ++R RN IN +S VKI  +E D Y 
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYV 384

Query: 903 ATIDEKVTVKIGP----GHYEPPNGSKGWSLVIEGKDYKVWE 932
           A ID K+ VKIGP    G+  P N      +   G+DY VWE
Sbjct: 385 AKIDNKIMVKIGPKMDLGNLIPSN----LHVATSGQDYAVWE 421

BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 2.7e-108
Identity = 194/400 (48.50%), Postives = 257/400 (64.25%), Query Frame = 0

Query: 542 EILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 601
           ++L QGFNWES  K G WY  L EK  E++S G T +WLPPP+ SVSP+GYMP  LY+L+
Sbjct: 26  QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85

Query: 602 -SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIF-----GGRLNWDD 661
            S+YG   ELK  ++ FHD  ++ L D+V+NHRCA  K+  G++ +F      GRL+W  
Sbjct: 86  ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145

Query: 662 RAVVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDF 721
             + SDD  +  GRG++ +G  F AAP+IDH    V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205

Query: 722 VRGFWGGYVKDYLEASEPYFAVGEYWDSLSYT-YGEMDHNQDAHRQRIVDWINATNGTAG 781
            +G+     + Y++ + P F VGE W SL Y   G+   NQDA RQ +V+W+      A 
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265

Query: 782 AFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 841
           AFD TTKGIL +A++  E WRL D  GK PG++GW P +AVTF++NHDTGSTQ  W FP 
Sbjct: 266 AFDFTTKGILQAAVQG-ELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPS 325

Query: 842 GKEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDF 901
            K MQGYAYILTHPG P +F+DHVF  + + EIA L  +R RN I+  S + I KAE D 
Sbjct: 326 DKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385

Query: 902 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWE 932
           Y A ID KV  K+GP +         + +V  G DY VWE
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424

BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match: A2YGY2 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 4.6e-108
Identity = 203/413 (49.15%), Postives = 263/413 (63.68%), Query Frame = 0

Query: 536 GTGSGFEILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPK 595
           G  SG +IL QGFNWES  +SG WY  L  K  ++ + G T +WLPPP+ SVS +GYMP 
Sbjct: 17  GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76

Query: 596 DLYNLN-SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIF-----GG 655
            LY+L+ SRYG   ELK  +   H   I+ + DVV+NHRCA  K+  GI+ IF      G
Sbjct: 77  RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136

Query: 656 RLNWDDRAVVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRK-EIGY 715
           RL+W    +  DD  F  G GN  +G +F AAP+IDH    V+ ++ +WL WL+  E+G+
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196

Query: 716 DGWRLDFVRGFWGGYVKDYLEASEPY-FAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI 775
           D WRLDF RG+     K Y+E + P   AV E WDS++Y   G+ ++NQDAHRQ +VDW+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256

Query: 776 NATNGTAGA---FDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDT 835
           +   GTA A   FD TTKGI+++A+E  E WRL DQ+GK PGV+GWWP++AVTF++NHDT
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEG-ELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDT 316

Query: 836 GSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRS 895
           GSTQ  W FP  K MQGYAYILTHPG P +F+DH F    K +IAAL+ +RQRN +   S
Sbjct: 317 GSTQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATS 376

Query: 896 VVKIEKAERDFYAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
            +KI   + D Y A ID KV +KIG  +        G+ L   G  Y VWE S
Sbjct: 377 SLKIMLHDADAYVAEIDGKVVMKIGSRYDVSSLIPPGFHLAAHGNGYAVWEKS 428

BLAST of Spg025846 vs. ExPASy TrEMBL
Match: A0A5A7SY64 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003970 PE=4 SV=1)

HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 798/935 (85.35%), Postives = 852/935 (91.12%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+++D+  S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901

BLAST of Spg025846 vs. ExPASy TrEMBL
Match: A0A1S3BES1 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4 SV=1)

HSP 1 Score: 1668.7 bits (4320), Expect = 0.0e+00
Identity = 796/935 (85.13%), Postives = 848/935 (90.70%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+ +     +KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKXKTAEHLKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901

BLAST of Spg025846 vs. ExPASy TrEMBL
Match: A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 793/929 (85.36%), Postives = 847/929 (91.17%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL  GR++F+H+NSYRLP  
Sbjct: 1   MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
            PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP      
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIP  S GNLPDQQ +S+++D+  S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE 
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 895

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYK 929
           V VKIGPG++EPP+GS GWSLVIEGKDYK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYK 895

BLAST of Spg025846 vs. ExPASy TrEMBL
Match: A0A0A0KQJ3 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 PE=4 SV=1)

HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 794/934 (85.01%), Postives = 846/934 (90.58%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MS+I +DPLLYHC KGKHRFHHR RFN L+PCSF+YC NKLL  GR+SF+H+NSYR P +
Sbjct: 1   MSSIALDPLLYHCAKGKHRFHHRPRFNMLRPCSFTYCPNKLLCHGRKSFVHYNSYRPPTI 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           +A++ NA     FQSTDVL  ETFPLKR EKLEGRISVRLA+GKD + WELTVGCNL+GK
Sbjct: 61  KATTTNA---PTFQSTDVLFNETFPLKRNEKLEGRISVRLAQGKDHNNWELTVGCNLAGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIV 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GDVHEVKI++ 
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVHEVKIDLA 180

Query: 181 PDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLAV 240
           PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGN VG+ KGLGLWP       
Sbjct: 181 PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNKVGTKKGLGLWP------- 240

Query: 241 GQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKSL 300
                                   GALGQLSNLLVK E+N+K Q SS ESGDTKEEKKSL
Sbjct: 241 ------------------------GALGQLSNLLVKAETNSKDQGSSSESGDTKEEKKSL 300

Query: 301 EGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWEM 360
           EGFY+ELPIVKE+A DNS+SVSVRKC ETTKYLLYLE+DLPGDVIVHWGACR++T+KWE+
Sbjct: 301 EGFYKELPIVKEIAVDNSISVSVRKCSETTKYLLYLESDLPGDVIVHWGACRDDTKKWEI 360

Query: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKGD 420
           PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQ ENSWLNYKGD
Sbjct: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQKENSWLNYKGD 420

Query: 421 DFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
           DFYIPF S GNL +QQ +S+++DT  S+ SGEESEGVSVTAYTDGIIKEIRNLVTDISSQ
Sbjct: 421 DFYIPFPSSGNLSNQQRKSKLKDTRASKISGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480

Query: 481 KSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGSG 540
           K+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV ISSGTGSG
Sbjct: 481 KTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVRISSGTGSG 540

Query: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
           FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN
Sbjct: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600

Query: 601 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVSD 660
           SRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVSD
Sbjct: 601 SRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSD 660

Query: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGG 720
           DPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGG
Sbjct: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLLWLRKEIGYDGWRLDFVRGFWGG 720

Query: 721 YVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
           YVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG
Sbjct: 721 YVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780

Query: 781 ILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
           ILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA
Sbjct: 781 ILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840

Query: 841 YILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEKV 900
           Y+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAA IDE V
Sbjct: 841 YLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAAIIDETV 900

Query: 901 TVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
            VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 AVKIGPGNFEPPSGSNGWSLVIEGKDYKVWEVSK 900

BLAST of Spg025846 vs. ExPASy TrEMBL
Match: A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)

HSP 1 Score: 1654.0 bits (4282), Expect = 0.0e+00
Identity = 804/935 (85.99%), Postives = 845/935 (90.37%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
           MSTIG+DPLLYHCGKGKHRFH R +FNTLKPCSF Y  NKLLYRGRRSF + NSYRLP L
Sbjct: 1   MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60

Query: 61  RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
           R SS NAALLE+FQSTDVLLKETFPLKRTEK+EG I VRLA+GK+Q+QWELTVGCNL+GK
Sbjct: 61  RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120

Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
           WILHWGVSL+DD GSEWDQPPKEMIPPGSIPIKDYAIETPLKK SSSSLGD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180

Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
           VP+K IAAINFVLKDEETG WYQHKGRDF+VPL D HGDDGNIVG+ KGLGLWP      
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWP------ 240

Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
                                    GALGQLSNLLVK ES +KGQESSGESGDTKE  K 
Sbjct: 241 -------------------------GALGQLSNLLVKAES-SKGQESSGESGDTKE--KY 300

Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
           LEGFYEELPIVKEVA DNSVSVSVR CPETTKYLLYLETDLPGDVIVHWGA R+ETRKWE
Sbjct: 301 LEGFYEELPIVKEVAFDNSVSVSVRNCPETTKYLLYLETDLPGDVIVHWGASRDETRKWE 360

Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
           +PAAPHPPETTVFKNKALRTLLQ KEGGKG  GIFTIEEEF GFLFVLKQNEN WLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQLKEGGKGSCGIFTIEEEFDGFLFVLKQNENPWLNYKG 420

Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
           DDFYIPF S G+LP Q+ Q+E ED  VSRK+G+  E VSVTAYTDGIIKEIRN VTDISS
Sbjct: 421 DDFYIPFPSSGHLPGQKRQNEFEDAGVSRKAGDGYEEVSVTAYTDGIIKEIRNFVTDISS 480

Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
           Q++ KKKT EAQESILQEIEKLAAEAYSIFRS++PTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QRTQKKKTIEAQESILQEIEKLAAEAYSIFRSASPTFTEEIIEMPKPVEPPVQISSGTGS 540

Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
           GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTV+WLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVIWLPPPTESVSPEGYMPKDLYNL 600

Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
           +SRYGNIDELKD VKTFHDV IKVLGDVVLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 SSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660

Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
           DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLRWLRKEIGYDGWRLDFVRGFWG 720

Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
           GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDH+QDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHDQDAHRQRIVDWINATNGTAGAFDVTTK 780

Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
           GILHSAL+RCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840

Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
           AY LTHPGTPSVFFDH+FSHYKS+IAALI+LR+RNK+NCRSVVKIEKAERD YAATIDEK
Sbjct: 841 AYTLTHPGTPSVFFDHIFSHYKSDIAALISLRKRNKVNCRSVVKIEKAERDVYAATIDEK 900

Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
           V +KIGPG+YEPP+ S GWSLVIEG DYKVWE SK
Sbjct: 901 VAMKIGPGYYEPPSRSNGWSLVIEGNDYKVWEVSK 901

BLAST of Spg025846 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 606/942 (64.33%), Postives = 722/942 (76.65%), Query Frame = 0

Query: 1   MSTIGVDPLLYHCGKGKHRFHHRTRFN----TLKPCSFS----YCRNKLLYRGRRSFLHH 60
           MST+ ++ LL+H         H ++ N    +  P S +    +  NKLL+   +S    
Sbjct: 1   MSTVPIESLLHH-----SYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60

Query: 61  NSYRLPV-LRASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWEL 120
           +  + PV +RA+S + A++E  QS DV+ KE FP++R EK EG+I VRL   K+++ WEL
Sbjct: 61  SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120

Query: 121 TVGCNLSGKWILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD 180
           +VGC++ GKWILHWGVS V D GSEWDQPP++M PPGSI IKDYAIETPLKK S      
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGD--S 180

Query: 181 VHEVKIEIVPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGL 240
             EV I +  + ++AA+NFVLKDEETG+WYQHKGRDF+VPL+D   D+GN++G+ KG   
Sbjct: 181 FFEVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF-- 240

Query: 241 WPGVVVLAVGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESG 300
                                            GALGQLSN+ +K       Q+ S    
Sbjct: 241 ---------------------------------GALGQLSNIPLK-------QDKSSAET 300

Query: 301 DTKEEKKSLEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGAC 360
           D+ EE+K L+ FYEE+PI K VA DNSVSV+ RKCPET+K ++ +ETDLPGDV VHWG C
Sbjct: 301 DSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVC 360

Query: 361 RNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNE 420
           +N T+KWE+P+ P+P ET++FKNKALRT LQ K+ G G  G+F+++ +  G  FVLK NE
Sbjct: 361 KNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNE 420

Query: 421 NSWLNYKGDDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIR 480
           N+WLNY+G+DFY+PF     L    S  E E   VS+   +  + VS + +T  II EIR
Sbjct: 421 NTWLNYRGEDFYVPF-----LTSSSSPVETEAAQVSKPKRKTDKEVSASGFTKEIITEIR 480

Query: 481 NLVTDISSQKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV 540
           NL  DISS K+ K   KE QE+ILQEIEKLAAEAYSIFRS+ P F+EE +   +  +P +
Sbjct: 481 NLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDI 540

Query: 541 PISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGY 600
            ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA EL+SLGFTVLWLPPPTESVSPEGY
Sbjct: 541 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 600

Query: 601 MPKDLYNLNSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLN 660
           MPKDLYNLNSRYG IDELKD VK FH V IKVLGD VLNHRCAH KNQNG+WN+FGGRLN
Sbjct: 601 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 660

Query: 661 WDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRL 720
           WDDRAVV+DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVR DIKEWLCW+ +E+GYDGWRL
Sbjct: 661 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 720

Query: 721 DFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTA 780
           DFVRGFWGGYVKDY++AS+PYFAVGEYWDSLSYTYGEMD+NQDAHRQRIVDWINAT+G A
Sbjct: 721 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 780

Query: 781 GAFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
           GAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP
Sbjct: 781 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840

Query: 841 GGKEMQGYAYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDF 900
            GKEMQGYAYILTHPGTP+VFFDH+FS Y SEIAAL++LR R K++CRS V I+K+ERD 
Sbjct: 841 EGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDV 887

Query: 901 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
           YAA IDEKV +KIGPGHYEPPNGS+ WS+ +EG+DYKVWE S
Sbjct: 901 YAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887

BLAST of Spg025846 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 421.4 bits (1082), Expect = 1.9e-117
Identity = 193/395 (48.86%), Postives = 267/395 (67.59%), Query Frame = 0

Query: 540 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 599
           G E++ Q +NWESHK   W+  L  K  +++  GFT  WLPPP++S++PEGY+P+DLY+L
Sbjct: 24  GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83

Query: 600 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-RLNWDDRAVV 659
           NS YG+   LK  ++     +++ + D+V+NHR   ++   G++N + G  L WD+ AV 
Sbjct: 84  NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143

Query: 660 SDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 719
           S      G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+ 
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203

Query: 720 GGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 779
             YVK+Y+ A++P F+VGE WDS +Y    +D+NQD+HRQRI+ WI+AT   + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263

Query: 780 KGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 839
           KGIL  A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323

Query: 840 YAYILTHPGTPSVFFDHVF---SHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAAT 899
           YAYILTHPG P VF+DH +   S    +I  LI +R+R  I+ RS V++ KAE + YAA 
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383

Query: 900 IDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVW 931
           + EK+ +K+G G + P    + W+L   G  Y VW
Sbjct: 384 VGEKICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411

BLAST of Spg025846 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 350.1 bits (897), Expect = 5.4e-96
Identity = 180/399 (45.11%), Postives = 242/399 (60.65%), Query Frame = 0

Query: 543 ILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN- 602
           +L Q FNWES  K G +Y  L     ++++ G T LWLPPP++SV+PEGY+P  LY+LN 
Sbjct: 27  LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86

Query: 603 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-----RLNWDDR 662
           S+YG+  ELK  +K  +   IK L D+V+NHR A  K+    +  F G     RL+WD  
Sbjct: 87  SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146

Query: 663 AVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVR 722
            V  +DP F G GN  +G +F  AP+IDH    V+ ++ EW+ WL+ EIG+ GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206

Query: 723 GFWGGYVKDYLEASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI-NATNGTAGA 782
           G+     K Y++ + P FAVGE WD + Y   G++D++Q+ HR  +  WI  A  G   A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266

Query: 783 FDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGG 842
           FD TTKGIL SA+ + E WRL D +GKPPG++G  P  AVTFI+NHDT  T   W FP  
Sbjct: 267 FDFTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSD 326

Query: 843 KEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDFY 902
           K + GY YILTHPGTP +F++H      K  I+ L+ +R +N I   S V I+ AE D Y
Sbjct: 327 KVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLY 386

Query: 903 AATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWE 932
            A ID+KV +KIGP           ++L   G D+ VWE
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421

BLAST of Spg025846 vs. TAIR 10
Match: AT1G10760.1 (Pyruvate phosphate dikinase, PEP/pyruvate binding domain )

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 0

Query: 123 LHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIVPD 182
           LHWG  L  D    W  P +   P  +   K+ A+ TP  K   +S      +K+EI  D
Sbjct: 120 LHWGAIL--DNKENWVLPSRS--PDRTQNFKNSALRTPFVKSGGNS-----HLKLEI-DD 179

Query: 183 KTIAAINFVLKDEETGSWYQHKGRDFRVPL 213
             I AI F++ DE    WY++ G++F + L
Sbjct: 180 PAIHAIEFLIFDESRNKWYKNNGQNFHINL 199

BLAST of Spg025846 vs. TAIR 10
Match: AT4G24450.1 (phosphoglucan, water dikinase )

HSP 1 Score: 44.7 bits (104), Expect = 4.8e-04
Identity = 24/98 (24.49%), Postives = 45/98 (45.92%), Query Frame = 0

Query: 334 LYLETDLPGDVIVHWGACRNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGI 393
           L++ T++ G  ++HWG  ++   +W +P     PE + F + A +T        +     
Sbjct: 271 LWIATNMAGPTVLHWGVAKSSAGEWLIPPPDVLPEKSKFVHGACQTQFTDMSSREHSYQF 330

Query: 394 FTIEEEFGGFL---FVLKQNENSWLNYKGDDFYIPFSS 429
             I  + GGF+   FV+  +   W+N  G +F +   S
Sbjct: 331 IDINLKRGGFVGIQFVI-WSGGYWVNNNGANFVVNLKS 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891960.10.0e+0086.63alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida][more]
KAA0034391.10.0e+0085.35alpha-amylase 3 [Cucumis melo var. makuwa][more]
XP_008446314.10.0e+0085.13PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo][more]
XP_004135194.10.0e+0085.01alpha-amylase 3, chloroplastic [Cucumis sativus] >KGN51888.1 hypothetical protei... [more]
TYK15529.10.0e+0085.36alpha-amylase 3 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q94A410.0e+0064.33Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
Q8LFG12.7e-11648.86Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
P178595.8e-11150.25Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1[more]
P279342.7e-10848.50Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
A2YGY24.6e-10849.15Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A5A7SY640.0e+0085.351,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S3BES10.0e+0085.131,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4... [more]
A0A5D3CZB50.0e+0085.36Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 P... [more]
A0A0A0KQJ30.0e+0085.011,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 P... [more]
A0A6J1DCP40.0e+0085.991,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110191... [more]
Match NameE-valueIdentityDescription
AT1G69830.10.0e+0064.33alpha-amylase-like 3 [more]
AT1G76130.11.9e-11748.86alpha-amylase-like 2 [more]
AT4G25000.15.4e-9645.11alpha-amylase-like [more]
AT1G10760.11.3e-0433.33Pyruvate phosphate dikinase, PEP/pyruvate binding domain [more]
AT4G24450.14.8e-0424.49phosphoglucan, water dikinase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 484..504
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 542..875
e-value: 2.8E-125
score: 420.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..297
NoneNo IPR availablePANTHERPTHR43447:SF33ALPHA-AMYLASE 3, CHLOROPLASTICcoord: 81..933
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 81..933
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 543..882
e-value: 4.39328E-171
score: 497.514
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 876..931
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 872..932
e-value: 5.4E-27
score: 105.7
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 873..931
e-value: 1.3E-21
score: 76.6
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 542..871
e-value: 8.8E-41
score: 151.5
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 569..822
e-value: 9.2E-16
score: 58.3
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 876..932
e-value: 2.2E-19
score: 71.3
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 543..859

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025846.1Spg025846.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0003824 catalytic activity