Homology
BLAST of Spg025846 vs. NCBI nr
Match:
XP_038891960.1 (alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1693.7 bits (4385), Expect = 0.0e+00
Identity = 810/935 (86.63%), Postives = 855/935 (91.44%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MSTI +DPLLYHCGKGKHRFH+R RFNTLKPCSFSYC NKLL+ GR+SF+++NSYRLP
Sbjct: 1 MSTISLDPLLYHCGKGKHRFHYRPRFNTLKPCSFSYCPNKLLHHGRKSFVNYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
RAS+ NA LL+ FQS DVL +ETFPLKR EKLEGRISVRLA+G DQ+QWELTVGCNL+GK
Sbjct: 61 RASATNAPLLQTFQSKDVLFQETFPLKRNEKLEGRISVRLAQGNDQNQWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSIPIKDYAIETPLKK SSSS GD VHEVKI +
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSSGDFVHEVKINL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
VPDKTIAAINFVLKDE+TG WYQHKGRDF+VPLLDYHG+DGNIVG+ KGLGLWP
Sbjct: 181 VPDKTIAAINFVLKDEQTGIWYQHKGRDFKVPLLDYHGEDGNIVGTKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK ES++K + SGESGD KEEKK
Sbjct: 241 -------------------------GALGQLSNLLVKAESSSKDKGRSGESGDAKEEKKY 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNSVSVSVRKCPETTK LLYLETDLPGDVIVHWGACR+ETRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSVSVSVRKCPETTKNLLYLETDLPGDVIVHWGACRDETRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGC GIFTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCCGIFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S+ GNLP QQ +SE++D VS+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLSNSGNLPGQQRESELKDAGVSKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEE+IE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEVIEMPKPVEPPVQISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKE+AAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKERAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGDVVLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPCGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AYILTHPGTPS F+DH+FSHYKSEIAALI+LR+RNK+NCRSVV IEKAERD YAA IDEK
Sbjct: 841 AYILTHPGTPSAFYDHIFSHYKSEIAALISLRKRNKVNCRSVVTIEKAERDVYAAIIDEK 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 904
BLAST of Spg025846 vs. NCBI nr
Match:
KAA0034391.1 (alpha-amylase 3 [Cucumis melo var. makuwa])
HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 798/935 (85.35%), Postives = 852/935 (91.12%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+++D+ S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901
BLAST of Spg025846 vs. NCBI nr
Match:
XP_008446314.1 (PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo])
HSP 1 Score: 1668.7 bits (4320), Expect = 0.0e+00
Identity = 796/935 (85.13%), Postives = 848/935 (90.70%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+ + +KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKXKTAEHLKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901
BLAST of Spg025846 vs. NCBI nr
Match:
XP_004135194.1 (alpha-amylase 3, chloroplastic [Cucumis sativus] >KGN51888.1 hypothetical protein Csa_008336 [Cucumis sativus])
HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 794/934 (85.01%), Postives = 846/934 (90.58%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC KGKHRFHHR RFN L+PCSF+YC NKLL GR+SF+H+NSYR P +
Sbjct: 1 MSSIALDPLLYHCAKGKHRFHHRPRFNMLRPCSFTYCPNKLLCHGRKSFVHYNSYRPPTI 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL ETFPLKR EKLEGRISVRLA+GKD + WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFNETFPLKRNEKLEGRISVRLAQGKDHNNWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIV 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GDVHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVHEVKIDLA 180
Query: 181 PDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLAV 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGN VG+ KGLGLWP
Sbjct: 181 PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNKVGTKKGLGLWP------- 240
Query: 241 GQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKSL 300
GALGQLSNLLVK E+N+K Q SS ESGDTKEEKKSL
Sbjct: 241 ------------------------GALGQLSNLLVKAETNSKDQGSSSESGDTKEEKKSL 300
Query: 301 EGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWEM 360
EGFY+ELPIVKE+A DNS+SVSVRKC ETTKYLLYLE+DLPGDVIVHWGACR++T+KWE+
Sbjct: 301 EGFYKELPIVKEIAVDNSISVSVRKCSETTKYLLYLESDLPGDVIVHWGACRDDTKKWEI 360
Query: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKGD 420
PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQ ENSWLNYKGD
Sbjct: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQKENSWLNYKGD 420
Query: 421 DFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
DFYIPF S GNL +QQ +S+++DT S+ SGEESEGVSVTAYTDGIIKEIRNLVTDISSQ
Sbjct: 421 DFYIPFPSSGNLSNQQRKSKLKDTRASKISGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
Query: 481 KSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGSG 540
K+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV ISSGTGSG
Sbjct: 481 KTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVRISSGTGSG 540
Query: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN
Sbjct: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
Query: 601 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVSD 660
SRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVSD
Sbjct: 601 SRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSD 660
Query: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGG 720
DPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGG
Sbjct: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLLWLRKEIGYDGWRLDFVRGFWGG 720
Query: 721 YVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
YVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG
Sbjct: 721 YVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
Query: 781 ILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
ILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA
Sbjct: 781 ILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
Query: 841 YILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEKV 900
Y+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAA IDE V
Sbjct: 841 YLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAAIIDETV 900
Query: 901 TVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 AVKIGPGNFEPPSGSNGWSLVIEGKDYKVWEVSK 900
BLAST of Spg025846 vs. NCBI nr
Match:
TYK15529.1 (alpha-amylase 3 [Cucumis melo var. makuwa])
HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 793/929 (85.36%), Postives = 847/929 (91.17%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+++D+ S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 895
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYK 929
V VKIGPG++EPP+GS GWSLVIEGKDYK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYK 895
BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match:
Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)
HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 606/942 (64.33%), Postives = 722/942 (76.65%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFN----TLKPCSFS----YCRNKLLYRGRRSFLHH 60
MST+ ++ LL+H H ++ N + P S + + NKLL+ +S
Sbjct: 1 MSTVPIESLLHH-----SYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60
Query: 61 NSYRLPV-LRASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWEL 120
+ + PV +RA+S + A++E QS DV+ KE FP++R EK EG+I VRL K+++ WEL
Sbjct: 61 SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120
Query: 121 TVGCNLSGKWILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD 180
+VGC++ GKWILHWGVS V D GSEWDQPP++M PPGSI IKDYAIETPLKK S
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGD--S 180
Query: 181 VHEVKIEIVPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGL 240
EV I + + ++AA+NFVLKDEETG+WYQHKGRDF+VPL+D D+GN++G+ KG
Sbjct: 181 FFEVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF-- 240
Query: 241 WPGVVVLAVGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESG 300
GALGQLSN+ +K Q+ S
Sbjct: 241 ---------------------------------GALGQLSNIPLK-------QDKSSAET 300
Query: 301 DTKEEKKSLEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGAC 360
D+ EE+K L+ FYEE+PI K VA DNSVSV+ RKCPET+K ++ +ETDLPGDV VHWG C
Sbjct: 301 DSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVC 360
Query: 361 RNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNE 420
+N T+KWE+P+ P+P ET++FKNKALRT LQ K+ G G G+F+++ + G FVLK NE
Sbjct: 361 KNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNE 420
Query: 421 NSWLNYKGDDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIR 480
N+WLNY+G+DFY+PF L S E E VS+ + + VS + +T II EIR
Sbjct: 421 NTWLNYRGEDFYVPF-----LTSSSSPVETEAAQVSKPKRKTDKEVSASGFTKEIITEIR 480
Query: 481 NLVTDISSQKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV 540
NL DISS K+ K KE QE+ILQEIEKLAAEAYSIFRS+ P F+EE + + +P +
Sbjct: 481 NLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDI 540
Query: 541 PISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGY 600
ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA EL+SLGFTVLWLPPPTESVSPEGY
Sbjct: 541 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 600
Query: 601 MPKDLYNLNSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLN 660
MPKDLYNLNSRYG IDELKD VK FH V IKVLGD VLNHRCAH KNQNG+WN+FGGRLN
Sbjct: 601 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 660
Query: 661 WDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRL 720
WDDRAVV+DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVR DIKEWLCW+ +E+GYDGWRL
Sbjct: 661 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 720
Query: 721 DFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTA 780
DFVRGFWGGYVKDY++AS+PYFAVGEYWDSLSYTYGEMD+NQDAHRQRIVDWINAT+G A
Sbjct: 721 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 780
Query: 781 GAFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
GAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP
Sbjct: 781 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
Query: 841 GGKEMQGYAYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDF 900
GKEMQGYAYILTHPGTP+VFFDH+FS Y SEIAAL++LR R K++CRS V I+K+ERD
Sbjct: 841 EGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDV 887
Query: 901 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
YAA IDEKV +KIGPGHYEPPNGS+ WS+ +EG+DYKVWE S
Sbjct: 901 YAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887
BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match:
Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)
HSP 1 Score: 421.4 bits (1082), Expect = 2.7e-116
Identity = 193/395 (48.86%), Postives = 267/395 (67.59%), Query Frame = 0
Query: 540 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 599
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 600 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-RLNWDDRAVV 659
NS YG+ LK ++ +++ + D+V+NHR ++ G++N + G L WD+ AV
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 660 SDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 719
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 720 GGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 779
YVK+Y+ A++P F+VGE WDS +Y +D+NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 780 KGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 839
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 840 YAYILTHPGTPSVFFDHVF---SHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAAT 899
YAYILTHPG P VF+DH + S +I LI +R+R I+ RS V++ KAE + YAA
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 900 IDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVW 931
+ EK+ +K+G G + P + W+L G Y VW
Sbjct: 384 VGEKICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411
BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match:
P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)
HSP 1 Score: 403.7 bits (1036), Expect = 5.8e-111
Identity = 202/402 (50.25%), Postives = 264/402 (65.67%), Query Frame = 0
Query: 543 ILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN-S 602
+L QGFNWES K G WY LK +L++ G T +WLPPP++SVSPEGY+P LY+L+ S
Sbjct: 25 LLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDAS 84
Query: 603 RYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-----RLNWDDRA 662
+YG+ +ELK + FH+ IK L D+V+NHR A K+ GI+ IF G R +W
Sbjct: 85 KYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSF 144
Query: 663 VVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVR 722
+ DD + G GN SG+ + AAP+IDH V+ ++ EW+ WL+ EIG+DGWR DFV+
Sbjct: 145 ICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVK 204
Query: 723 GFWGGYVKDYLEASEPYFAVGEYWDSLSYTY-GEMDHNQDAHRQRIVDWINATNGTAGAF 782
G+ K Y+E ++P FAVGE WDS+SY G+ ++NQD+HR +V+W+ + G AF
Sbjct: 205 GYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPNYNQDSHRGALVNWVESAGGAITAF 264
Query: 783 DVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGK 842
D TTKGIL +A++ E WRL D GKPPG++G P AVTFI+NHDTGSTQ W FP K
Sbjct: 265 DFTTKGILQAAVQG-ELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDK 324
Query: 843 EMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDFYA 902
MQGYAYILTHPGTPS+F+DH F K +IA L ++R RN IN +S VKI +E D Y
Sbjct: 325 VMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYV 384
Query: 903 ATIDEKVTVKIGP----GHYEPPNGSKGWSLVIEGKDYKVWE 932
A ID K+ VKIGP G+ P N + G+DY VWE
Sbjct: 385 AKIDNKIMVKIGPKMDLGNLIPSN----LHVATSGQDYAVWE 421
BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match:
P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)
HSP 1 Score: 394.8 bits (1013), Expect = 2.7e-108
Identity = 194/400 (48.50%), Postives = 257/400 (64.25%), Query Frame = 0
Query: 542 EILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 601
++L QGFNWES K G WY L EK E++S G T +WLPPP+ SVSP+GYMP LY+L+
Sbjct: 26 QVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLD 85
Query: 602 -SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIF-----GGRLNWDD 661
S+YG ELK ++ FHD ++ L D+V+NHRCA K+ G++ +F GRL+W
Sbjct: 86 ASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGP 145
Query: 662 RAVVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDF 721
+ SDD + GRG++ +G F AAP+IDH V+ ++ +WL WLR ++G+DGWRLDF
Sbjct: 146 DMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDF 205
Query: 722 VRGFWGGYVKDYLEASEPYFAVGEYWDSLSYT-YGEMDHNQDAHRQRIVDWINATNGTAG 781
+G+ + Y++ + P F VGE W SL Y G+ NQDA RQ +V+W+ A
Sbjct: 206 AKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPAT 265
Query: 782 AFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPG 841
AFD TTKGIL +A++ E WRL D GK PG++GW P +AVTF++NHDTGSTQ W FP
Sbjct: 266 AFDFTTKGILQAAVQG-ELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPS 325
Query: 842 GKEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDF 901
K MQGYAYILTHPG P +F+DHVF + + EIA L +R RN I+ S + I KAE D
Sbjct: 326 DKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDI 385
Query: 902 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWE 932
Y A ID KV K+GP + + +V G DY VWE
Sbjct: 386 YVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424
BLAST of Spg025846 vs. ExPASy Swiss-Prot
Match:
A2YGY2 (Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV=1)
HSP 1 Score: 394.0 bits (1011), Expect = 4.6e-108
Identity = 203/413 (49.15%), Postives = 263/413 (63.68%), Query Frame = 0
Query: 536 GTGSGFEILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPK 595
G SG +IL QGFNWES +SG WY L K ++ + G T +WLPPP+ SVS +GYMP
Sbjct: 17 GLASGDKILFQGFNWESWRQSGGWYNLLMGKVDDIVAAGVTHVWLPPPSHSVSTQGYMPG 76
Query: 596 DLYNLN-SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIF-----GG 655
LY+L+ SRYG ELK + H I+ + DVV+NHRCA K+ GI+ IF G
Sbjct: 77 RLYDLDASRYGTSMELKSLISALHGKGIQAIADVVINHRCADYKDSRGIYCIFEGGTPDG 136
Query: 656 RLNWDDRAVVSDDPHF-QGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRK-EIGY 715
RL+W + DD F G GN +G +F AAP+IDH V+ ++ +WL WL+ E+G+
Sbjct: 137 RLDWGPHMICRDDTQFSDGTGNLDTGADFAAAPDIDHLNGVVQRELTDWLLWLKSDEVGF 196
Query: 716 DGWRLDFVRGFWGGYVKDYLEASEPY-FAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI 775
D WRLDF RG+ K Y+E + P AV E WDS++Y G+ ++NQDAHRQ +VDW+
Sbjct: 197 DAWRLDFARGYSPEVAKVYIEGTTPVGLAVAELWDSMAYGGDGKPEYNQDAHRQALVDWV 256
Query: 776 NATNGTAGA---FDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDT 835
+ GTA A FD TTKGI+++A+E E WRL DQ+GK PGV+GWWP++AVTF++NHDT
Sbjct: 257 DRVGGTASAGMVFDFTTKGIMNTAVEG-ELWRLIDQQGKAPGVIGWWPAKAVTFVDNHDT 316
Query: 836 GSTQGHWRFPGGKEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRS 895
GSTQ W FP K MQGYAYILTHPG P +F+DH F K +IAAL+ +RQRN + S
Sbjct: 317 GSTQQMWPFPSDKVMQGYAYILTHPGNPCIFYDHFFDWGLKEQIAALVAVRQRNGVTATS 376
Query: 896 VVKIEKAERDFYAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
+KI + D Y A ID KV +KIG + G+ L G Y VWE S
Sbjct: 377 SLKIMLHDADAYVAEIDGKVVMKIGSRYDVSSLIPPGFHLAAHGNGYAVWEKS 428
BLAST of Spg025846 vs. ExPASy TrEMBL
Match:
A0A5A7SY64 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003970 PE=4 SV=1)
HSP 1 Score: 1676.0 bits (4339), Expect = 0.0e+00
Identity = 798/935 (85.35%), Postives = 852/935 (91.12%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+++D+ S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901
BLAST of Spg025846 vs. ExPASy TrEMBL
Match:
A0A1S3BES1 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4 SV=1)
HSP 1 Score: 1668.7 bits (4320), Expect = 0.0e+00
Identity = 796/935 (85.13%), Postives = 848/935 (90.70%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+ + +KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKXKTAEHLKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYKVWEVSK 901
BLAST of Spg025846 vs. ExPASy TrEMBL
Match:
A0A5D3CZB5 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 PE=3 SV=1)
HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 793/929 (85.36%), Postives = 847/929 (91.17%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC K KHRFHHR RFN LKPCSF+YC NKLL GR++F+H+NSYRLP
Sbjct: 1 MSSIALDPLLYHCAKAKHRFHHRPRFNMLKPCSFTYCPNKLLCHGRKTFVHYNSYRLPTF 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL KETFPLKR EKLEGRISV+LA+GKD ++WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFKETFPLKRNEKLEGRISVKLAQGKDHNKWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGNIVG+ KGLGLWP
Sbjct: 181 APDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNIVGAKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK E+N+K Q SS ESGDTKE+KKS
Sbjct: 241 -------------------------GALGQLSNLLVKTETNSKDQGSSSESGDTKEDKKS 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNS+SVSV+KCPETTKYLLYLE+DLPGDV+VHWGACR++TRKWE
Sbjct: 301 LEGFYEELPIVKEVAVDNSISVSVKKCPETTKYLLYLESDLPGDVVVHWGACRDDTRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQNENSWLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQNENSWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIP S GNLPDQQ +S+++D+ S+KSGEESEGVSVTAYTDGIIKEIRNLVTDISS
Sbjct: 421 DDFYIPLPSSGNLPDQQRKSKLKDSGASKKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
QK+ KKKTKEAQE+ILQEIEKLAAEAYSIFRSSAPTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QKTKKKKTKEAQENILQEIEKLAAEAYSIFRSSAPTFTEEIIEMPKPVEPPVRISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
NSRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 NSRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAATIDE
Sbjct: 841 AYLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDET 895
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYK 929
V VKIGPG++EPP+GS GWSLVIEGKDYK
Sbjct: 901 VAVKIGPGNFEPPSGSSGWSLVIEGKDYK 895
BLAST of Spg025846 vs. ExPASy TrEMBL
Match:
A0A0A0KQJ3 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 PE=4 SV=1)
HSP 1 Score: 1664.4 bits (4309), Expect = 0.0e+00
Identity = 794/934 (85.01%), Postives = 846/934 (90.58%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MS+I +DPLLYHC KGKHRFHHR RFN L+PCSF+YC NKLL GR+SF+H+NSYR P +
Sbjct: 1 MSSIALDPLLYHCAKGKHRFHHRPRFNMLRPCSFTYCPNKLLCHGRKSFVHYNSYRPPTI 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
+A++ NA FQSTDVL ETFPLKR EKLEGRISVRLA+GKD + WELTVGCNL+GK
Sbjct: 61 KATTTNA---PTFQSTDVLFNETFPLKRNEKLEGRISVRLAQGKDHNNWELTVGCNLAGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIV 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSI IKDYAIETPLKK SSSS GDVHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSITIKDYAIETPLKKSSSSSSGDVHEVKIDLA 180
Query: 181 PDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLAV 240
PDKTIAAINFVLKDEETG WYQHKGRDF+VPLLDY G+DGN VG+ KGLGLWP
Sbjct: 181 PDKTIAAINFVLKDEETGIWYQHKGRDFKVPLLDYCGEDGNKVGTKKGLGLWP------- 240
Query: 241 GQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKSL 300
GALGQLSNLLVK E+N+K Q SS ESGDTKEEKKSL
Sbjct: 241 ------------------------GALGQLSNLLVKAETNSKDQGSSSESGDTKEEKKSL 300
Query: 301 EGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWEM 360
EGFY+ELPIVKE+A DNS+SVSVRKC ETTKYLLYLE+DLPGDVIVHWGACR++T+KWE+
Sbjct: 301 EGFYKELPIVKEIAVDNSISVSVRKCSETTKYLLYLESDLPGDVIVHWGACRDDTKKWEI 360
Query: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKGD 420
PAAPHPPETTVFKNKALRTLLQPKEGGKGCSG+FTIEE+FGGFLFVLKQ ENSWLNYKGD
Sbjct: 361 PAAPHPPETTVFKNKALRTLLQPKEGGKGCSGVFTIEEDFGGFLFVLKQKENSWLNYKGD 420
Query: 421 DFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
DFYIPF S GNL +QQ +S+++DT S+ SGEESEGVSVTAYTDGIIKEIRNLVTDISSQ
Sbjct: 421 DFYIPFPSSGNLSNQQRKSKLKDTRASKISGEESEGVSVTAYTDGIIKEIRNLVTDISSQ 480
Query: 481 KSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGSG 540
K+ KKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV ISSGTGSG
Sbjct: 481 KTKKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVRISSGTGSG 540
Query: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN
Sbjct: 541 FEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN 600
Query: 601 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVSD 660
SRYGNIDELKD VKTFHDV IKVLGD VLNHRCAH KNQNGIWNIFGGRLNWDDRAVVSD
Sbjct: 601 SRYGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSD 660
Query: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGG 720
DPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGG
Sbjct: 661 DPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLLWLRKEIGYDGWRLDFVRGFWGG 720
Query: 721 YVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
YVKDYL+ASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG
Sbjct: 721 YVKDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKG 780
Query: 781 ILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
ILHSAL+RCEYWRLSD+KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA
Sbjct: 781 ILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYA 840
Query: 841 YILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEKV 900
Y+LTHPGTPSVF+DH+FSHYKSEIAALI+LR+RNK+NCRSVVKI KAERD YAA IDE V
Sbjct: 841 YLLTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAAIIDETV 900
Query: 901 TVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
VKIGPG++EPP+GS GWSLVIEGKDYKVWE SK
Sbjct: 901 AVKIGPGNFEPPSGSNGWSLVIEGKDYKVWEVSK 900
BLAST of Spg025846 vs. ExPASy TrEMBL
Match:
A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)
HSP 1 Score: 1654.0 bits (4282), Expect = 0.0e+00
Identity = 804/935 (85.99%), Postives = 845/935 (90.37%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFNTLKPCSFSYCRNKLLYRGRRSFLHHNSYRLPVL 60
MSTIG+DPLLYHCGKGKHRFH R +FNTLKPCSF Y NKLLYRGRRSF + NSYRLP L
Sbjct: 1 MSTIGLDPLLYHCGKGKHRFHRRPQFNTLKPCSFGYYPNKLLYRGRRSFFNCNSYRLPAL 60
Query: 61 RASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWELTVGCNLSGK 120
R SS NAALLE+FQSTDVLLKETFPLKRTEK+EG I VRLA+GK+Q+QWELTVGCNL+GK
Sbjct: 61 RXSSTNAALLESFQSTDVLLKETFPLKRTEKVEGTIFVRLAQGKNQNQWELTVGCNLTGK 120
Query: 121 WILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD-VHEVKIEI 180
WILHWGVSL+DD GSEWDQPPKEMIPPGSIPIKDYAIETPLKK SSSSLGD VHEVKI++
Sbjct: 121 WILHWGVSLIDDSGSEWDQPPKEMIPPGSIPIKDYAIETPLKKSSSSSLGDTVHEVKIDL 180
Query: 181 VPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGLWPGVVVLA 240
VP+K IAAINFVLKDEETG WYQHKGRDF+VPL D HGDDGNIVG+ KGLGLWP
Sbjct: 181 VPNKGIAAINFVLKDEETGIWYQHKGRDFKVPLTDSHGDDGNIVGTKKGLGLWP------ 240
Query: 241 VGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESGDTKEEKKS 300
GALGQLSNLLVK ES +KGQESSGESGDTKE K
Sbjct: 241 -------------------------GALGQLSNLLVKAES-SKGQESSGESGDTKE--KY 300
Query: 301 LEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGACRNETRKWE 360
LEGFYEELPIVKEVA DNSVSVSVR CPETTKYLLYLETDLPGDVIVHWGA R+ETRKWE
Sbjct: 301 LEGFYEELPIVKEVAFDNSVSVSVRNCPETTKYLLYLETDLPGDVIVHWGASRDETRKWE 360
Query: 361 MPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNENSWLNYKG 420
+PAAPHPPETTVFKNKALRTLLQ KEGGKG GIFTIEEEF GFLFVLKQNEN WLNYKG
Sbjct: 361 IPAAPHPPETTVFKNKALRTLLQLKEGGKGSCGIFTIEEEFDGFLFVLKQNENPWLNYKG 420
Query: 421 DDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIRNLVTDISS 480
DDFYIPF S G+LP Q+ Q+E ED VSRK+G+ E VSVTAYTDGIIKEIRN VTDISS
Sbjct: 421 DDFYIPFPSSGHLPGQKRQNEFEDAGVSRKAGDGYEEVSVTAYTDGIIKEIRNFVTDISS 480
Query: 481 QKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPVPISSGTGS 540
Q++ KKKT EAQESILQEIEKLAAEAYSIFRS++PTFTEEIIE PKPVEPPV ISSGTGS
Sbjct: 481 QRTQKKKTIEAQESILQEIEKLAAEAYSIFRSASPTFTEEIIEMPKPVEPPVQISSGTGS 540
Query: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 600
GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTV+WLPPPTESVSPEGYMPKDLYNL
Sbjct: 541 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVIWLPPPTESVSPEGYMPKDLYNL 600
Query: 601 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLNWDDRAVVS 660
+SRYGNIDELKD VKTFHDV IKVLGDVVLNHRCAH KNQNGIWNIFGGRLNWDDRAVVS
Sbjct: 601 SSRYGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVS 660
Query: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWG 720
DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVRNDIKEWL WLRKEIGYDGWRLDFVRGFWG
Sbjct: 661 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLRWLRKEIGYDGWRLDFVRGFWG 720
Query: 721 GYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTK 780
GYVKDYL+ASEPYFAVGEYWDSLSYTYGEMDH+QDAHRQRIVDWINATNGTAGAFDVTTK
Sbjct: 721 GYVKDYLDASEPYFAVGEYWDSLSYTYGEMDHDQDAHRQRIVDWINATNGTAGAFDVTTK 780
Query: 781 GILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
GILHSAL+RCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY
Sbjct: 781 GILHSALDRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGY 840
Query: 841 AYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAATIDEK 900
AY LTHPGTPSVFFDH+FSHYKS+IAALI+LR+RNK+NCRSVVKIEKAERD YAATIDEK
Sbjct: 841 AYTLTHPGTPSVFFDHIFSHYKSDIAALISLRKRNKVNCRSVVKIEKAERDVYAATIDEK 900
Query: 901 VTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEASK 935
V +KIGPG+YEPP+ S GWSLVIEG DYKVWE SK
Sbjct: 901 VAMKIGPGYYEPPSRSNGWSLVIEGNDYKVWEVSK 901
BLAST of Spg025846 vs. TAIR 10
Match:
AT1G69830.1 (alpha-amylase-like 3 )
HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 606/942 (64.33%), Postives = 722/942 (76.65%), Query Frame = 0
Query: 1 MSTIGVDPLLYHCGKGKHRFHHRTRFN----TLKPCSFS----YCRNKLLYRGRRSFLHH 60
MST+ ++ LL+H H ++ N + P S + + NKLL+ +S
Sbjct: 1 MSTVPIESLLHH-----SYLRHNSKVNRGNRSFIPISLNLRSHFTSNKLLHSIGKSVGVS 60
Query: 61 NSYRLPV-LRASSPNAALLEAFQSTDVLLKETFPLKRTEKLEGRISVRLARGKDQSQWEL 120
+ + PV +RA+S + A++E QS DV+ KE FP++R EK EG+I VRL K+++ WEL
Sbjct: 61 SMNKSPVAIRATSSDTAVVETAQSDDVIFKEIFPVQRIEKAEGKIYVRLKEVKEKN-WEL 120
Query: 121 TVGCNLSGKWILHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGD 180
+VGC++ GKWILHWGVS V D GSEWDQPP++M PPGSI IKDYAIETPLKK S
Sbjct: 121 SVGCSIPGKWILHWGVSYVGDTGSEWDQPPEDMRPPGSIAIKDYAIETPLKKLSEGD--S 180
Query: 181 VHEVKIEIVPDKTIAAINFVLKDEETGSWYQHKGRDFRVPLLDYHGDDGNIVGSIKGLGL 240
EV I + + ++AA+NFVLKDEETG+WYQHKGRDF+VPL+D D+GN++G+ KG
Sbjct: 181 FFEVAINLNLESSVAALNFVLKDEETGAWYQHKGRDFKVPLVDDVPDNGNLIGAKKGF-- 240
Query: 241 WPGVVVLAVGQQDILTIVYLMWVGCTFLISSIVGALGQLSNLLVKPESNTKGQESSGESG 300
GALGQLSN+ +K Q+ S
Sbjct: 241 ---------------------------------GALGQLSNIPLK-------QDKSSAET 300
Query: 301 DTKEEKKSLEGFYEELPIVKEVAADNSVSVSVRKCPETTKYLLYLETDLPGDVIVHWGAC 360
D+ EE+K L+ FYEE+PI K VA DNSVSV+ RKCPET+K ++ +ETDLPGDV VHWG C
Sbjct: 301 DSIEERKGLQEFYEEMPISKRVADDNSVSVTARKCPETSKNIVSIETDLPGDVTVHWGVC 360
Query: 361 RNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGIFTIEEEFGGFLFVLKQNE 420
+N T+KWE+P+ P+P ET++FKNKALRT LQ K+ G G G+F+++ + G FVLK NE
Sbjct: 361 KNGTKKWEIPSEPYPEETSLFKNKALRTRLQRKDDGNGSFGLFSLDGKLEGLCFVLKLNE 420
Query: 421 NSWLNYKGDDFYIPFSSFGNLPDQQSQSEIEDTVVSRKSGEESEGVSVTAYTDGIIKEIR 480
N+WLNY+G+DFY+PF L S E E VS+ + + VS + +T II EIR
Sbjct: 421 NTWLNYRGEDFYVPF-----LTSSSSPVETEAAQVSKPKRKTDKEVSASGFTKEIITEIR 480
Query: 481 NLVTDISSQKSPKKKTKEAQESILQEIEKLAAEAYSIFRSSAPTFTEEIIETPKPVEPPV 540
NL DISS K+ K KE QE+ILQEIEKLAAEAYSIFRS+ P F+EE + + +P +
Sbjct: 481 NLAIDISSHKNQKTNVKEVQENILQEIEKLAAEAYSIFRSTTPAFSEEGVLEAEADKPDI 540
Query: 541 PISSGTGSGFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGY 600
ISSGTGSGFEILCQGFNWES+KSGRWY+EL+EKA EL+SLGFTVLWLPPPTESVSPEGY
Sbjct: 541 KISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGY 600
Query: 601 MPKDLYNLNSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGGRLN 660
MPKDLYNLNSRYG IDELKD VK FH V IKVLGD VLNHRCAH KNQNG+WN+FGGRLN
Sbjct: 601 MPKDLYNLNSRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLN 660
Query: 661 WDDRAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRL 720
WDDRAVV+DDPHFQGRGNKSSGDNFHAAPNIDHSQ+FVR DIKEWLCW+ +E+GYDGWRL
Sbjct: 661 WDDRAVVADDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 720
Query: 721 DFVRGFWGGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTA 780
DFVRGFWGGYVKDY++AS+PYFAVGEYWDSLSYTYGEMD+NQDAHRQRIVDWINAT+G A
Sbjct: 721 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAA 780
Query: 781 GAFDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
GAFDVTTKGILH+AL++CEYWRLSD KGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP
Sbjct: 781 GAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP 840
Query: 841 GGKEMQGYAYILTHPGTPSVFFDHVFSHYKSEIAALITLRQRNKINCRSVVKIEKAERDF 900
GKEMQGYAYILTHPGTP+VFFDH+FS Y SEIAAL++LR R K++CRS V I+K+ERD
Sbjct: 841 EGKEMQGYAYILTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDV 887
Query: 901 YAATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWEAS 934
YAA IDEKV +KIGPGHYEPPNGS+ WS+ +EG+DYKVWE S
Sbjct: 901 YAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887
BLAST of Spg025846 vs. TAIR 10
Match:
AT1G76130.1 (alpha-amylase-like 2 )
HSP 1 Score: 421.4 bits (1082), Expect = 1.9e-117
Identity = 193/395 (48.86%), Postives = 267/395 (67.59%), Query Frame = 0
Query: 540 GFEILCQGFNWESHKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNL 599
G E++ Q +NWESHK W+ L K +++ GFT WLPPP++S++PEGY+P+DLY+L
Sbjct: 24 GREVILQAYNWESHKYD-WWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSL 83
Query: 600 NSRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-RLNWDDRAVV 659
NS YG+ LK ++ +++ + D+V+NHR ++ G++N + G L WD+ AV
Sbjct: 84 NSAYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVT 143
Query: 660 SDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFW 719
S G GN+S+GDNF+ PN+DH+Q FVR DI WL WLR +G+ +R DF RG+
Sbjct: 144 SCT---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYS 203
Query: 720 GGYVKDYLEASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTT 779
YVK+Y+ A++P F+VGE WDS +Y +D+NQD+HRQRI+ WI+AT + AFD TT
Sbjct: 204 ANYVKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTT 263
Query: 780 KGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQG 839
KGIL A+ + +YWRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP M+G
Sbjct: 264 KGILQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEG 323
Query: 840 YAYILTHPGTPSVFFDHVF---SHYKSEIAALITLRQRNKINCRSVVKIEKAERDFYAAT 899
YAYILTHPG P VF+DH + S +I LI +R+R I+ RS V++ KAE + YAA
Sbjct: 324 YAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAI 383
Query: 900 IDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVW 931
+ EK+ +K+G G + P + W+L G Y VW
Sbjct: 384 VGEKICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411
BLAST of Spg025846 vs. TAIR 10
Match:
AT4G25000.1 (alpha-amylase-like )
HSP 1 Score: 350.1 bits (897), Expect = 5.4e-96
Identity = 180/399 (45.11%), Postives = 242/399 (60.65%), Query Frame = 0
Query: 543 ILCQGFNWES-HKSGRWYMELKEKAAELSSLGFTVLWLPPPTESVSPEGYMPKDLYNLN- 602
+L Q FNWES K G +Y L ++++ G T LWLPPP++SV+PEGY+P LY+LN
Sbjct: 27 LLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNS 86
Query: 603 SRYGNIDELKDAVKTFHDVEIKVLGDVVLNHRCAHSKNQNGIWNIFGG-----RLNWDDR 662
S+YG+ ELK +K + IK L D+V+NHR A K+ + F G RL+WD
Sbjct: 87 SKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPS 146
Query: 663 AVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRNDIKEWLCWLRKEIGYDGWRLDFVR 722
V +DP F G GN +G +F AP+IDH V+ ++ EW+ WL+ EIG+ GWR D+VR
Sbjct: 147 FVCRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVR 206
Query: 723 GFWGGYVKDYLEASEPYFAVGEYWDSLSY-TYGEMDHNQDAHRQRIVDWI-NATNGTAGA 782
G+ K Y++ + P FAVGE WD + Y G++D++Q+ HR + WI A G A
Sbjct: 207 GYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTA 266
Query: 783 FDVTTKGILHSALERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGG 842
FD TTKGIL SA+ + E WRL D +GKPPG++G P AVTFI+NHDT T W FP
Sbjct: 267 FDFTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSD 326
Query: 843 KEMQGYAYILTHPGTPSVFFDHVFS-HYKSEIAALITLRQRNKINCRSVVKIEKAERDFY 902
K + GY YILTHPGTP +F++H K I+ L+ +R +N I S V I+ AE D Y
Sbjct: 327 KVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLY 386
Query: 903 AATIDEKVTVKIGPGHYEPPNGSKGWSLVIEGKDYKVWE 932
A ID+KV +KIGP ++L G D+ VWE
Sbjct: 387 LAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421
BLAST of Spg025846 vs. TAIR 10
Match:
AT1G10760.1 (Pyruvate phosphate dikinase, PEP/pyruvate binding domain )
HSP 1 Score: 46.6 bits (109), Expect = 1.3e-04
Identity = 30/90 (33.33%), Postives = 44/90 (48.89%), Query Frame = 0
Query: 123 LHWGVSLVDDIGSEWDQPPKEMIPPGSIPIKDYAIETPLKKPSSSSLGDVHEVKIEIVPD 182
LHWG L D W P + P + K+ A+ TP K +S +K+EI D
Sbjct: 120 LHWGAIL--DNKENWVLPSRS--PDRTQNFKNSALRTPFVKSGGNS-----HLKLEI-DD 179
Query: 183 KTIAAINFVLKDEETGSWYQHKGRDFRVPL 213
I AI F++ DE WY++ G++F + L
Sbjct: 180 PAIHAIEFLIFDESRNKWYKNNGQNFHINL 199
BLAST of Spg025846 vs. TAIR 10
Match:
AT4G24450.1 (phosphoglucan, water dikinase )
HSP 1 Score: 44.7 bits (104), Expect = 4.8e-04
Identity = 24/98 (24.49%), Postives = 45/98 (45.92%), Query Frame = 0
Query: 334 LYLETDLPGDVIVHWGACRNETRKWEMPAAPHPPETTVFKNKALRTLLQPKEGGKGCSGI 393
L++ T++ G ++HWG ++ +W +P PE + F + A +T +
Sbjct: 271 LWIATNMAGPTVLHWGVAKSSAGEWLIPPPDVLPEKSKFVHGACQTQFTDMSSREHSYQF 330
Query: 394 FTIEEEFGGFL---FVLKQNENSWLNYKGDDFYIPFSS 429
I + GGF+ FV+ + W+N G +F + S
Sbjct: 331 IDINLKRGGFVGIQFVI-WSGGYWVNNNGANFVVNLKS 367
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038891960.1 | 0.0e+00 | 86.63 | alpha-amylase 3, chloroplastic isoform X1 [Benincasa hispida] | [more] |
KAA0034391.1 | 0.0e+00 | 85.35 | alpha-amylase 3 [Cucumis melo var. makuwa] | [more] |
XP_008446314.1 | 0.0e+00 | 85.13 | PREDICTED: LOW QUALITY PROTEIN: alpha-amylase 3, chloroplastic [Cucumis melo] | [more] |
XP_004135194.1 | 0.0e+00 | 85.01 | alpha-amylase 3, chloroplastic [Cucumis sativus] >KGN51888.1 hypothetical protei... | [more] |
TYK15529.1 | 0.0e+00 | 85.36 | alpha-amylase 3 [Cucumis melo var. makuwa] | [more] |
Match Name | E-value | Identity | Description | |
Q94A41 | 0.0e+00 | 64.33 | Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1 | [more] |
Q8LFG1 | 2.7e-116 | 48.86 | Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1 | [more] |
P17859 | 5.8e-111 | 50.25 | Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1 | [more] |
P27934 | 2.7e-108 | 48.50 | Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... | [more] |
A2YGY2 | 4.6e-108 | 49.15 | Alpha-amylase isozyme 2A OS=Oryza sativa subsp. indica OX=39946 GN=AMYC2 PE=2 SV... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7SY64 | 0.0e+00 | 85.35 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... | [more] |
A0A1S3BES1 | 0.0e+00 | 85.13 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4... | [more] |
A0A5D3CZB5 | 0.0e+00 | 85.36 | Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00210 P... | [more] |
A0A0A0KQJ3 | 0.0e+00 | 85.01 | 1,4-alpha-D-glucan glucanohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G604330 P... | [more] |
A0A6J1DCP4 | 0.0e+00 | 85.99 | 1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110191... | [more] |