Spg025780 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025780 vs. NCBI nr
Match: XP_023529184.1 (WAT1-related protein At1g70260-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.7 bits (1576), Expect = 4.2e-171
Identity = 313/364 (85.99%), Postives = 341/364 (93.68%), Query Frame = 0

Query: 5   GQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFH 64
           G ++W V+PFLAMV+LEGCTI LTI+AKTAITYG +TFVFVVYTNAVAS +LLPYSF+FH
Sbjct: 6   GALLWGVVPFLAMVTLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFH 65

Query: 65  FNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFI 124
           FNDRT+YQQSLFSFPLLLRIFLLG TGIC+SQNLAFLGLSYSSPIVVC MGLMLPAISFI
Sbjct: 66  FNDRTDYQQSLFSFPLLLRIFLLGFTGICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFI 125

Query: 125 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFV 184
           LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL V
Sbjct: 126 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLV 185

Query: 185 FTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFF 244
           FTSTTDRWI+GGLLLAAA+LCVSIWNIIQLGV+KQYSQVMKVASFYS VG+ +SA +AFF
Sbjct: 186 FTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFF 245

Query: 245 VVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 304
           +VDD +AW IKS FD+FLIIATG+FSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF
Sbjct: 246 IVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 305

Query: 305 GATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNKISSDDLCSAKVPLLDE 364
           G+TFFGDTFHYGSVMAAFIAGMGY TVMWGQMNE+   ++++N    DDL S++VPLLDE
Sbjct: 306 GSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESN----DDLSSSRVPLLDE 365

Query: 365 ESKV 369
           ESKV
Sbjct: 366 ESKV 365

BLAST of Spg025780 vs. NCBI nr
Match: XP_022957168.1 (WAT1-related protein At1g70260-like [Cucurbita moschata])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 320/369 (86.72%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 1   MEFKGQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYS 60
           ME K Q IW V+PFLAMVSLEGCTIALTIMAKTAITYGM+TFVFVVYTNAVAS +LLPYS
Sbjct: 1   MEVKKQ-IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYS 60

Query: 61  FIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPA 120
           FIFH+N+RTE+QQSLFSFPL LR+FLLGL GIC+SQNLAFLGLSYSSPIVVCAMGLMLPA
Sbjct: 61  FIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPA 120

Query: 121 ISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQ 180
           ISF+LSILL RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK 
Sbjct: 121 ISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKH 180

Query: 181 KLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSAT 240
           KL VFTSTTDRWI+GGLLLAAASLCVSIWNI+QLGVIKQY QVMKVASFYS VGT LSA 
Sbjct: 181 KLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLSAA 240

Query: 241 VAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300
           V FFVV+D SAW IKSSFDLFLIIATGIFS LIRNRVQIWCMQMKGPYYVPMFKPFGILF
Sbjct: 241 VGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300

Query: 301 ATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKNNKISSDDLCSAKV 360
           ATFFG TFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+ G GE+K++K ++D L SAK+
Sbjct: 301 ATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDK-NNDHLSSAKL 360

Query: 361 PLLDEESKV 369
           PLLDEESKV
Sbjct: 361 PLLDEESKV 367

BLAST of Spg025780 vs. NCBI nr
Match: KAG6601366.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608.6 bits (1568), Expect = 3.6e-170
Identity = 319/369 (86.45%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 1   MEFKGQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYS 60
           ME K Q IW V+PFLAMVSLEGCTIALTIMAKTAITYGM+TFVFVVYTNAVAS +LLPYS
Sbjct: 1   MEVKKQ-IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYS 60

Query: 61  FIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPA 120
           FIFH+N+RTE+QQSLFSFPL LR+FLLGL GIC+SQNLAFLGLSYSSPIVVCAMGLMLPA
Sbjct: 61  FIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPA 120

Query: 121 ISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQ 180
           ISF+LSI+L RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK 
Sbjct: 121 ISFLLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKH 180

Query: 181 KLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSAT 240
           KL VFTSTTDRWI+GGLLLAAASLCVSIWNI+QLGVIKQY QVMKVASFYS VGT LSA 
Sbjct: 181 KLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLSAA 240

Query: 241 VAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300
           V FFVV+D SAW IKSSFDLFLIIATGIFS LIRNRVQIWCMQMKGPYYVPMFKPFGILF
Sbjct: 241 VGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300

Query: 301 ATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKNNKISSDDLCSAKV 360
           ATFFG TFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+ G GE+K++K ++D L SAK+
Sbjct: 301 ATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDK-NNDHLSSAKL 360

Query: 361 PLLDEESKV 369
           PLLDEESKV
Sbjct: 361 PLLDEESKV 367

BLAST of Spg025780 vs. NCBI nr
Match: KAG6600128.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 606.3 bits (1562), Expect = 1.8e-169
Identity = 311/364 (85.44%), Postives = 338/364 (92.86%), Query Frame = 0

Query: 5   GQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFH 64
           G ++W V+PFLAMVSLEGCTI LTI+AKTAITYG +TFVFVVYTNAVAS +LLPYSF+FH
Sbjct: 6   GALLWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFH 65

Query: 65  FNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFI 124
            NDRT+YQQSLFS PLLLRIFLLG  GIC+SQNLAFLGLSYSSPIVVC MGLMLPAISFI
Sbjct: 66  LNDRTDYQQSLFSLPLLLRIFLLGFIGICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFI 125

Query: 125 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFV 184
           LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL V
Sbjct: 126 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLV 185

Query: 185 FTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFF 244
           FTSTTDRWI+GGLLLAAA+LCVSIWNIIQLGV+KQYSQVMKVASFYS VG+ +SA ++FF
Sbjct: 186 FTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMSFF 245

Query: 245 VVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 304
           +VDD +AW IKS FD+FLIIATG+FSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF
Sbjct: 246 IVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 305

Query: 305 GATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNKISSDDLCSAKVPLLDE 364
           G+TFFGDTFHYGSVMAAFIAGMGY TVMWGQMNE+   ++K+N    DDL S++VPLLDE
Sbjct: 306 GSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDKSN----DDLSSSRVPLLDE 365

Query: 365 ESKV 369
           ESKV
Sbjct: 366 ESKV 365

BLAST of Spg025780 vs. NCBI nr
Match: XP_038892671.1 (WAT1-related protein At1g70260 [Benincasa hispida])

HSP 1 Score: 606.3 bits (1562), Expect = 1.8e-169
Identity = 320/370 (86.49%), Postives = 340/370 (91.89%), Query Frame = 0

Query: 1   MEFKGQ-IIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPY 60
           ME KG+ I+  VIPFLAMVS+EGCTIALTI+AKTAITYGMSTFVFVVYTNAVAS ILLPY
Sbjct: 1   MEVKGERILGTVIPFLAMVSMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60

Query: 61  SFIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFH+N R EYQQSLFSFPLLLR+FLLG TGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNHRMEYQQSLFSFPLLLRVFLLGFTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISFILSILL RTKI WK+P+FITKVVGTVISVIGAT EELYLGPVVRQHPSSS HLQFK
Sbjct: 121 AISFILSILLGRTKIEWKNPNFITKVVGTVISVIGATCEELYLGPVVRQHPSSSTHLQFK 180

Query: 181 QKLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSA 240
           QKL VFTSTTDRWI GGLLLAAA+LCVSIWNIIQLGVIKQYSQVMKVASFYS VGT LS 
Sbjct: 181 QKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVIKQYSQVMKVASFYSSVGTLLST 240

Query: 241 TVAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
            VA+FVV+D SAW IKS+FDLFLIIATG FSGLIRNRVQIWCMQ KGPYYVPMFKPFGIL
Sbjct: 241 IVAYFVVNDSSAWTIKSNFDLFLIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGIL 300

Query: 301 FATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKNNKISSDDLCSAK 360
           FATFFG TFFGDTFHYGSVMAAFIAGMGY TV+WGQ+NE+ G GE+K+   ++DDL SAK
Sbjct: 301 FATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVLWGQINEDRGVGEDKDGDKNNDDLSSAK 360

Query: 361 VPLLDEESKV 369
           VPLLDEES+V
Sbjct: 361 VPLLDEESEV 370

BLAST of Spg025780 vs. ExPASy Swiss-Prot
Match: F4I5D5 (WAT1-related protein At1g70260 OS=Arabidopsis thaliana OX=3702 GN=At1g70260 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 5.3e-100
Identity = 201/368 (54.62%), Postives = 263/368 (71.47%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           ++PF+AM  +E CTIALTIMAKTA+T GMS FVFVVYTNA  S +LLP+SF+FH N+RTE
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             QS+FS+PLL+R+F LG TGI + QNLAF+GL +SSPIVVCAMGL +P+ SF+LSI+L 
Sbjct: 70  --QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILG 129

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLH--LQFKQKLFVFTST 190
           R+K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   KL V+ + 
Sbjct: 130 RSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNL 189

Query: 191 TDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVDD 250
            D W LG + LA A   VS++N++Q G +K+Y  VMKVASFYS VGT      + F+  D
Sbjct: 190 PDNWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERD 249

Query: 251 PSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGATF 310
            SAW+I+ +FDL+LIIATG F  +IR  V + C QMKGPYYVP+FKPFGI +AT FG +F
Sbjct: 250 LSAWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSF 309

Query: 311 FGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNK------ISSDDLCSAKVPLL 369
           F ++ HYGSV+ A IAG+GY+TV WGQ+ E  E +  N +      I   D    KVPLL
Sbjct: 310 FVNSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDEDEYKVPLL 369

BLAST of Spg025780 vs. ExPASy Swiss-Prot
Match: Q9ZUI8 (WAT1-related protein At1g60050 OS=Arabidopsis thaliana OX=3702 GN=At1g60050 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 7.1e-89
Identity = 181/351 (51.57%), Postives = 238/351 (67.81%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           ++PF+ M  +E CTIALTI+AKTA+T GMS FVF+VYTNA+ S +LLPYSF FH ++  +
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILL- 130
             +   + P L+RIFLLG TG+ + QN+AFLGLSYSSPIVVCAMGL  PA SF+LS+ L 
Sbjct: 71  --EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALG 130

Query: 131 RRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL---FVFT 190
           +   + W S     +V+GT+I   GA  E +YLGP +R  P SS    F   +     F 
Sbjct: 131 KEGGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 191 STTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVV 250
             +D W LG LLLA A+L +SIWNIIQL  +++Y QVMKV S YS  GT   A  + F+ 
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFME 250

Query: 251 DDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGA 310
            D SAW +K + DL+LIIATGIF  +IR  VQ+ C +MKGPYYVP+FKPFGIL+A+ FG 
Sbjct: 251 PDLSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGT 310

Query: 311 TFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGE----EKNNKISSDD 354
           +FF ++ HYGSV+ A IAG GY  +MW Q+ ++   E      N+++ SD+
Sbjct: 311 SFFVNSLHYGSVLGAAIAGTGYLLIMWSQVQKDDPNETVEKNDNHQLDSDE 359

BLAST of Spg025780 vs. ExPASy Swiss-Prot
Match: Q94JU2 (WAT1-related protein At3g28050 OS=Arabidopsis thaliana OX=3702 GN=At3g28050 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.2e-48
Identity = 129/364 (35.44%), Postives = 193/364 (53.02%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+P  A+V +E   + L  + K A   GMS  VF+VY+  +A+ +LLP S    F  RT 
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLP-SLFCSFRSRT- 69

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
                 +F +L +I LLG+ G C S  + + G++YSSP +  A+  + PA +F+L+++ R
Sbjct: 70  --LPPMNFSILYKIVLLGIIG-CCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFR 129

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVV--RQHPSSSLHLQFKQKLFVFTST 190
              +++K  S + K++GTV+S+ GA    LY GPVV  +  PS SL  Q         ST
Sbjct: 130 MESVSFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------ST 189

Query: 191 TDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVV-D 250
              WILG   LA    CV +W I+Q  ++++Y     V  FYS   +F +A V  F   +
Sbjct: 190 NPNWILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGN 249

Query: 251 DPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGAT 310
           D  AW+IK +  L  I+ +G+F   I N +  W +++KGP +V MFKP  I  A   G  
Sbjct: 250 DLGAWKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVI 309

Query: 311 FFGDTFHYGSVMAAFIAGMGYYTVMWGQMNE--------EGEGEEKNNKISSDDLCSAKV 364
           F  D+ + GS++ A +  +G+YTVMWG+  E        +   EE N         S K 
Sbjct: 310 FLRDSLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKA 359

BLAST of Spg025780 vs. ExPASy Swiss-Prot
Match: Q9FL08 (WAT1-related protein At5g40240 OS=Arabidopsis thaliana OX=3702 GN=At5g40240 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.0e-47
Identity = 116/329 (35.26%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+PF AM ++E  T+    + K A   G+S +VFV Y+  V++ +LLP S IF  + R  
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             +S    PL  +IFLLGL G  +SQ     G++YSSP +  A+  + PA +F L+++ R
Sbjct: 78  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 137

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFVFTSTTD 190
             ++  +S +   K++G ++S+ GA    LY GP V    S +  L         TS   
Sbjct: 138 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIES 197

Query: 191 RWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVDDPS 250
            WI+GGLLLA+    +S+W I+Q  V++ Y + + V  FY+   T +S  V  F   + +
Sbjct: 198 SWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLT 257

Query: 251 AWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGATFFG 310
           +W +K    L  II +G+F  L       W + +KGP Y+ +F+P  I  A   GA F G
Sbjct: 258 SWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLG 317

Query: 311 DTFHYGSVMAAFIAGMGYYTVMWGQMNEE 340
           D  H GSV+ + I  +G+YTV+WG+  E+
Sbjct: 318 DALHLGSVIGSMILCIGFYTVIWGKARED 341

BLAST of Spg025780 vs. ExPASy Swiss-Prot
Match: F4JK59 (WAT1-related protein At4g15540 OS=Arabidopsis thaliana OX=3702 GN=At4g15540 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-44
Identity = 123/358 (34.36%), Postives = 186/358 (51.96%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+PF AM+++E  T+  +I+ K A   G S +VFV Y    A+ +LL  S IF  +    
Sbjct: 15  VVPFTAMIAIECTTVGSSILYKAATLRGFSFYVFVFYAYVGATLVLLLLSLIFGRSRSLP 74

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             +S     L  +IFLL L G+  S+     G+ YSSP +  A+  + PA +FIL+I  R
Sbjct: 75  TAKS----SLFFKIFLLALLGL-TSRVAGCKGIEYSSPTLSSAISNLTPAFTFILAIFFR 134

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFV---FTS 190
             ++  +S +   K++GT++S+ GA    LY GP                KL V   FTS
Sbjct: 135 MEQVMLRSSATQAKIIGTIVSISGALVIVLYKGP----------------KLLVAASFTS 194

Query: 191 TTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVD 250
               WI+GGLLL    L +S+W I+Q  +++ Y + + V   Y+   T +S TV   V  
Sbjct: 195 FESSWIIGGLLLGLQFLLLSVWFILQTHIMEIYPEEIAVVFCYNLCATLISGTVCLLVEK 254

Query: 251 DPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGAT 310
           D ++W++K  F L  +I +G+F   + + +  W + +KGP Y+ +FKP  I  A    A 
Sbjct: 255 DLNSWQLKPGFSLASVIYSGLFDTSLGSVIHTWGLHVKGPVYISLFKPLSIAIAVAMAAI 314

Query: 311 FFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNKISSDDLCSAKVPLLDEE 366
           F GDT H GSV+ + I   G+YTV+WG+  E+      + K  SD   S  +P  D E
Sbjct: 315 FLGDTLHLGSVIGSVILSFGFYTVIWGKARED------STKTVSDSEQSLLLPSHDRE 345

BLAST of Spg025780 vs. ExPASy TrEMBL
Match: A0A6J1GZG2 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458639 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.7e-171
Identity = 320/369 (86.72%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 1   MEFKGQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYS 60
           ME K Q IW V+PFLAMVSLEGCTIALTIMAKTAITYGM+TFVFVVYTNAVAS +LLPYS
Sbjct: 1   MEVKKQ-IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYS 60

Query: 61  FIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPA 120
           FIFH+N+RTE+QQSLFSFPL LR+FLLGL GIC+SQNLAFLGLSYSSPIVVCAMGLMLPA
Sbjct: 61  FIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPA 120

Query: 121 ISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQ 180
           ISF+LSILL RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK 
Sbjct: 121 ISFVLSILLGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKH 180

Query: 181 KLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSAT 240
           KL VFTSTTDRWI+GGLLLAAASLCVSIWNI+QLGVIKQY QVMKVASFYS VGT LSA 
Sbjct: 181 KLLVFTSTTDRWIMGGLLLAAASLCVSIWNILQLGVIKQYPQVMKVASFYSLVGTLLSAA 240

Query: 241 VAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300
           V FFVV+D SAW IKSSFDLFLIIATGIFS LIRNRVQIWCMQMKGPYYVPMFKPFGILF
Sbjct: 241 VGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300

Query: 301 ATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKNNKISSDDLCSAKV 360
           ATFFG TFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+ G GE+K++K ++D L SAK+
Sbjct: 301 ATFFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDK-NNDHLSSAKL 360

Query: 361 PLLDEESKV 369
           PLLDEESKV
Sbjct: 361 PLLDEESKV 367

BLAST of Spg025780 vs. ExPASy TrEMBL
Match: A0A6J1FLY1 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111447017 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 311/364 (85.44%), Postives = 338/364 (92.86%), Query Frame = 0

Query: 5   GQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFH 64
           G ++W V+PFLAMVSLEGCTI LTI+AKTAITYG +TFVFVVYTNAVAS +LLPYSF+FH
Sbjct: 6   GALLWGVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLFH 65

Query: 65  FNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFI 124
            NDRT+YQQSLFS PLLLRIFLLG  GIC+SQNLAFLGLSYSSPIVVC MGLMLPAISFI
Sbjct: 66  LNDRTDYQQSLFSLPLLLRIFLLGFIGICVSQNLAFLGLSYSSPIVVCVMGLMLPAISFI 125

Query: 125 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFV 184
           LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL V
Sbjct: 126 LSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLLV 185

Query: 185 FTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFF 244
           FTSTTDRWI+GGLLLAAA+LCVSIWNIIQLGV+KQYSQVMKVASFYS VG+ +SA +AFF
Sbjct: 186 FTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVASFYSLVGSLMSAAMAFF 245

Query: 245 VVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 304
           +VDD +AW IKS FD+FLIIATG+FSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF
Sbjct: 246 IVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFF 305

Query: 305 GATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNKISSDDLCSAKVPLLDE 364
           G+TFFGDTFHYGSVMAAFIAGMGY TVMWGQMNE+   ++++N    DDL S++VPLLDE
Sbjct: 306 GSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQMNEDQGRDDESN----DDLSSSRVPLLDE 365

Query: 365 ESKV 369
           ESKV
Sbjct: 366 ESKV 365

BLAST of Spg025780 vs. ExPASy TrEMBL
Match: A0A6J1IKD8 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111476417 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.9e-169
Identity = 318/369 (86.18%), Postives = 340/369 (92.14%), Query Frame = 0

Query: 1   MEFKGQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYS 60
           ME K Q IW V+PFLAMVSLEGCTIALTIMAKTAITYGM+TFVFVVYTNAVAS +LLPYS
Sbjct: 1   MEVKKQ-IWGVVPFLAMVSLEGCTIALTIMAKTAITYGMTTFVFVVYTNAVASIVLLPYS 60

Query: 61  FIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPA 120
            IFH+N+RTE+QQSLFSFPL LR+FLLGL GIC+SQNLAFLGLSYSSPIVVCAMGLMLPA
Sbjct: 61  LIFHYNERTEFQQSLFSFPLFLRVFLLGLIGICVSQNLAFLGLSYSSPIVVCAMGLMLPA 120

Query: 121 ISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQ 180
           ISF+LSI+L RTKINWKSPSFITKVVGT+ISVIGATSEELY+GP VRQHPSSS HLQFK 
Sbjct: 121 ISFVLSIILGRTKINWKSPSFITKVVGTMISVIGATSEELYIGPAVRQHPSSSSHLQFKH 180

Query: 181 KLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSAT 240
           KL VFTSTTDRWI+GGLLLAAASLCVSIWNIIQLGVIKQY QVMKVASFYS VGT LSA 
Sbjct: 181 KLLVFTSTTDRWIMGGLLLAAASLCVSIWNIIQLGVIKQYPQVMKVASFYSLVGTLLSAA 240

Query: 241 VAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300
           V FFVV+D SAW IKSSFDLFLIIATGIFS LIRNRVQIWCMQMKGPYYVPMFKPFGILF
Sbjct: 241 VGFFVVNDSSAWTIKSSFDLFLIIATGIFSSLIRNRVQIWCMQMKGPYYVPMFKPFGILF 300

Query: 301 ATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKNNKISSDDLCSAKV 360
           AT FG TFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+ G GE+K++K ++D L SAK+
Sbjct: 301 ATLFGVTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDNGVGEDKDDK-NNDHLSSAKL 360

Query: 361 PLLDEESKV 369
           PLLDEESKV
Sbjct: 361 PLLDEESKV 367

BLAST of Spg025780 vs. ExPASy TrEMBL
Match: A0A6J1J595 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111483609 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.7e-168
Identity = 312/365 (85.48%), Postives = 337/365 (92.33%), Query Frame = 0

Query: 5   GQIIW-VVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIF 64
           G ++W VV+PFLAMVSLEGCTI LTI+AKTAITYG +TFVFVVYTNAVAS +LLPYSF+F
Sbjct: 6   GALLWGVVVPFLAMVSLEGCTIGLTILAKTAITYGTNTFVFVVYTNAVASFLLLPYSFLF 65

Query: 65  HFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISF 124
           H N RT+YQQSLFSFPLLLRIFLLG TGIC+SQNLAFLGLSYSSPIVVC MGLMLPAISF
Sbjct: 66  HLNHRTDYQQSLFSFPLLLRIFLLGFTGICVSQNLAFLGLSYSSPIVVCVMGLMLPAISF 125

Query: 125 ILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLF 184
           ILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHL FKQKL 
Sbjct: 126 ILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLHFKQKLL 185

Query: 185 VFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAF 244
           VFTSTTDRWI+GGLLLAAA+LCVSIWNIIQLGV+KQYSQ MKVASFYS VGT +SA +AF
Sbjct: 186 VFTSTTDRWIMGGLLLAAATLCVSIWNIIQLGVVKQYSQGMKVASFYSLVGTLMSAAMAF 245

Query: 245 FVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATF 304
           F VDD +AW IKS FD+FLIIATG+FSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATF
Sbjct: 246 FFVDDLNAWIIKSKFDIFLIIATGVFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATF 305

Query: 305 FGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNKISSDDLCSAKVPLLD 364
           FG+TFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+   ++K+N    DDL S++VPLLD
Sbjct: 306 FGSTFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDQGRDDKSN----DDLSSSRVPLLD 365

Query: 365 EESKV 369
           EESKV
Sbjct: 366 EESKV 366

BLAST of Spg025780 vs. ExPASy TrEMBL
Match: A0A0A0KQA2 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G602730 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 9.8e-166
Identity = 309/368 (83.97%), Postives = 339/368 (92.12%), Query Frame = 0

Query: 1   MEFK-GQIIWVVIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPY 60
           ME K G I+ +V+PFLAMV++EGCTIALTI+AKTAITYGMSTFVFVVYTNAVAS ILLPY
Sbjct: 1   MEVKGGGILEMVVPFLAMVTMEGCTIALTILAKTAITYGMSTFVFVVYTNAVASIILLPY 60

Query: 61  SFIFHFNDRTEYQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120
           SFIFH+N R E+QQSLFSFPLLLR+FLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP
Sbjct: 61  SFIFHYNQRLEFQQSLFSFPLLLRVFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLP 120

Query: 121 AISFILSILLRRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFK 180
           AISF+LSILL +TK+ WK+P+FITKVVGTVISV+GAT EE+YLGP VRQHPSSS HLQFK
Sbjct: 121 AISFLLSILLGKTKMEWKNPNFITKVVGTVISVVGATCEEVYLGPTVRQHPSSSTHLQFK 180

Query: 181 QKLFVFTSTTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSA 240
           QKL VFTSTTDRWI GGLLLAAA+LCVSIWNIIQLGV+KQYSQVMKV SFYS VGTF+SA
Sbjct: 181 QKLLVFTSTTDRWIFGGLLLAAATLCVSIWNIIQLGVVKQYSQVMKVGSFYSIVGTFMSA 240

Query: 241 TVAFFVVDDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGIL 300
            VA+FVV+D SAW +KSSFDL+LIIATG FSGLIRNRVQIWCMQ KGPYYVPMFKPFGIL
Sbjct: 241 IVAYFVVNDSSAWTVKSSFDLYLIIATGTFSGLIRNRVQIWCMQKKGPYYVPMFKPFGIL 300

Query: 301 FATFFGATFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEE-GEGEEKN-NKISSDDLCSA 360
           FATFFGATFFGDTFHYGSVMAAFIAGMGY TVMWGQ+NE+ G G++K+ NK + D L SA
Sbjct: 301 FATFFGATFFGDTFHYGSVMAAFIAGMGYLTVMWGQINEDRGVGKDKDINKNNDDSLSSA 360

Query: 361 KVPLLDEE 366
           KVPLLD+E
Sbjct: 361 KVPLLDDE 368

BLAST of Spg025780 vs. TAIR 10
Match: AT1G70260.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.7e-101
Identity = 201/368 (54.62%), Postives = 263/368 (71.47%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           ++PF+AM  +E CTIALTIMAKTA+T GMS FVFVVYTNA  S +LLP+SF+FH N+RTE
Sbjct: 10  LVPFVAMAIMEACTIALTIMAKTALTGGMSPFVFVVYTNAFGSILLLPFSFLFHRNERTE 69

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             QS+FS+PLL+R+F LG TGI + QNLAF+GL +SSPIVVCAMGL +P+ SF+LSI+L 
Sbjct: 70  --QSIFSWPLLVRVFFLGFTGIFMFQNLAFVGLRFSSPIVVCAMGLQIPSFSFLLSIILG 129

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLH--LQFKQKLFVFTST 190
           R+K++W++ S   K++GT++S+ GA  EELY GP +R   S+S +  L+   KL V+ + 
Sbjct: 130 RSKLDWRNTSTRAKLMGTIVSLSGAFVEELYKGPFIRPASSASPNRFLKSVPKLLVYYNL 189

Query: 191 TDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVDD 250
            D W LG + LA A   VS++N++Q G +K+Y  VMKVASFYS VGT      + F+  D
Sbjct: 190 PDNWFLGCIFLAVAVFSVSLFNVVQTGTVKKYPHVMKVASFYSIVGTIQCLLFSLFMERD 249

Query: 251 PSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGATF 310
            SAW+I+ +FDL+LIIATG F  +IR  V + C QMKGPYYVP+FKPFGI +AT FG +F
Sbjct: 250 LSAWKIQPNFDLYLIIATGTFGSVIRTSVHVKCTQMKGPYYVPLFKPFGIFWATLFGTSF 309

Query: 311 FGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGEEKNNK------ISSDDLCSAKVPLL 369
           F ++ HYGSV+ A IAG+GY+TV WGQ+ E  E +  N +      I   D    KVPLL
Sbjct: 310 FVNSLHYGSVLGAAIAGVGYFTVSWGQLKESEEKQSSNEERKSIKTIHHRDEDEYKVPLL 369

BLAST of Spg025780 vs. TAIR 10
Match: AT1G60050.1 (Nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 328.9 bits (842), Expect = 5.1e-90
Identity = 181/351 (51.57%), Postives = 238/351 (67.81%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           ++PF+ M  +E CTIALTI+AKTA+T GMS FVF+VYTNA+ S +LLPYSF FH ++  +
Sbjct: 11  IVPFIVMALMEACTIALTILAKTALTGGMSPFVFIVYTNALGSLLLLPYSFYFHRDESDD 70

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILL- 130
             +   + P L+RIFLLG TG+ + QN+AFLGLSYSSPIVVCAMGL  PA SF+LS+ L 
Sbjct: 71  --EPFLTKPSLVRIFLLGFTGVFLFQNMAFLGLSYSSPIVVCAMGLQSPAFSFLLSLALG 130

Query: 131 RRTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKL---FVFT 190
           +   + W S     +V+GT+I   GA  E +YLGP +R  P SS    F   +     F 
Sbjct: 131 KEGGLGWASKRTKGRVIGTLICFTGAFVEVIYLGPFIRPSPPSSPTSNFLTTISHYLTFF 190

Query: 191 STTDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVV 250
             +D W LG LLLA A+L +SIWNIIQL  +++Y QVMKV S YS  GT   A  + F+ 
Sbjct: 191 KNSDNWALGSLLLACATLSISIWNIIQLDTVQKYPQVMKVVSAYSLAGTLQCAIFSAFME 250

Query: 251 DDPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGA 310
            D SAW +K + DL+LIIATGIF  +IR  VQ+ C +MKGPYYVP+FKPFGIL+A+ FG 
Sbjct: 251 PDLSAWELKLNMDLYLIIATGIFGSIIRTSVQVKCSKMKGPYYVPLFKPFGILWASIFGT 310

Query: 311 TFFGDTFHYGSVMAAFIAGMGYYTVMWGQMNEEGEGE----EKNNKISSDD 354
           +FF ++ HYGSV+ A IAG GY  +MW Q+ ++   E      N+++ SD+
Sbjct: 311 SFFVNSLHYGSVLGAAIAGTGYLLIMWSQVQKDDPNETVEKNDNHQLDSDE 359

BLAST of Spg025780 vs. TAIR 10
Match: AT3G28050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 195.3 bits (495), Expect = 8.7e-50
Identity = 129/364 (35.44%), Postives = 193/364 (53.02%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+P  A+V +E   + L  + K A   GMS  VF+VY+  +A+ +LLP S    F  RT 
Sbjct: 10  VLPVTALVIMECANVGLNTLFKAATLKGMSFHVFIVYSYGLAALLLLP-SLFCSFRSRT- 69

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
                 +F +L +I LLG+ G C S  + + G++YSSP +  A+  + PA +F+L+++ R
Sbjct: 70  --LPPMNFSILYKIVLLGIIG-CCSNIMGYTGINYSSPTLASAISNLTPAFTFLLAVVFR 129

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVV--RQHPSSSLHLQFKQKLFVFTST 190
              +++K  S + K++GTV+S+ GA    LY GPVV  +  PS SL  Q         ST
Sbjct: 130 MESVSFKRTSSVAKMLGTVVSIGGAFIVTLYNGPVVIAKSPPSVSLRSQ---------ST 189

Query: 191 TDRWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVV-D 250
              WILG   LA    CV +W I+Q  ++++Y     V  FYS   +F +A V  F   +
Sbjct: 190 NPNWILGAGFLAVEYFCVPLWYIVQTQIMREYPAEFTVVCFYSIGVSFWTALVTLFTEGN 249

Query: 251 DPSAWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGAT 310
           D  AW+IK +  L  I+ +G+F   I N +  W +++KGP +V MFKP  I  A   G  
Sbjct: 250 DLGAWKIKPNIALVSIVCSGLFGSCINNTIHTWALRIKGPLFVAMFKPLSIAIAVAMGVI 309

Query: 311 FFGDTFHYGSVMAAFIAGMGYYTVMWGQMNE--------EGEGEEKNNKISSDDLCSAKV 364
           F  D+ + GS++ A +  +G+YTVMWG+  E        +   EE N         S K 
Sbjct: 310 FLRDSLYIGSLIGATVITIGFYTVMWGKAKEVALVEDDNKANHEEANEADLDSPSGSQKA 359

BLAST of Spg025780 vs. TAIR 10
Match: AT5G40240.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-49
Identity = 116/329 (35.26%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+PF AM ++E  T+    + K A   G+S +VFV Y+  V++ +LLP S IF  + R  
Sbjct: 18  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 77

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             +S    PL  +IFLLGL G  +SQ     G++YSSP +  A+  + PA +F L+++ R
Sbjct: 78  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 137

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFVFTSTTD 190
             ++  +S +   K++G ++S+ GA    LY GP V    S +  L         TS   
Sbjct: 138 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIES 197

Query: 191 RWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVDDPS 250
            WI+GGLLLA+    +S+W I+Q  V++ Y + + V  FY+   T +S  V  F   + +
Sbjct: 198 SWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLT 257

Query: 251 AWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGATFFG 310
           +W +K    L  II +G+F  L       W + +KGP Y+ +F+P  I  A   GA F G
Sbjct: 258 SWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLG 317

Query: 311 DTFHYGSVMAAFIAGMGYYTVMWGQMNEE 340
           D  H GSV+ + I  +G+YTV+WG+  E+
Sbjct: 318 DALHLGSVIGSMILCIGFYTVIWGKARED 341

BLAST of Spg025780 vs. TAIR 10
Match: AT5G40240.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-49
Identity = 116/329 (35.26%), Postives = 179/329 (54.41%), Query Frame = 0

Query: 11  VIPFLAMVSLEGCTIALTIMAKTAITYGMSTFVFVVYTNAVASTILLPYSFIFHFNDRTE 70
           V+PF AM ++E  T+    + K A   G+S +VFV Y+  V++ +LLP S IF  + R  
Sbjct: 32  VVPFAAMFAVECATVGSNTLFKAATLRGLSFYVFVFYSYIVSTLLLLPLSVIFGRSRRLP 91

Query: 71  YQQSLFSFPLLLRIFLLGLTGICISQNLAFLGLSYSSPIVVCAMGLMLPAISFILSILLR 130
             +S    PL  +IFLLGL G  +SQ     G++YSSP +  A+  + PA +F L+++ R
Sbjct: 92  AAKS----PLFFKIFLLGLVGF-MSQIAGCKGIAYSSPTLASAISNLTPAFTFTLAVIFR 151

Query: 131 RTKINWKSPSFITKVVGTVISVIGATSEELYLGPVVRQHPSSSLHLQFKQKLFVFTSTTD 190
             ++  +S +   K++G ++S+ GA    LY GP V    S +  L         TS   
Sbjct: 152 MEQVRLRSSATQAKIIGAILSISGALVVVLYKGPQVLASASFTTVLPTVTLHQQLTSIES 211

Query: 191 RWILGGLLLAAASLCVSIWNIIQLGVIKQYSQVMKVASFYSFVGTFLSATVAFFVVDDPS 250
            WI+GGLLLA+    +S+W I+Q  V++ Y + + V  FY+   T +S  V  F   + +
Sbjct: 212 SWIIGGLLLASQYFLISVWYILQTRVMEVYPEEITVVFFYNLFATLISVPVCLFAESNLT 271

Query: 251 AWRIKSSFDLFLIIATGIFSGLIRNRVQIWCMQMKGPYYVPMFKPFGILFATFFGATFFG 310
           +W +K    L  II +G+F  L       W + +KGP Y+ +F+P  I  A   GA F G
Sbjct: 272 SWVLKPDISLAAIIYSGVFVSLFSALTHTWGLHLKGPVYISLFRPLSIAIAVAMGAIFLG 331

Query: 311 DTFHYGSVMAAFIAGMGYYTVMWGQMNEE 340
           D  H GSV+ + I  +G+YTV+WG+  E+
Sbjct: 332 DALHLGSVIGSMILCIGFYTVIWGKARED 355