Homology
BLAST of Spg025690 vs. NCBI nr
Match:
XP_022954040.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])
HSP 1 Score: 550.8 bits (1418), Expect = 8.0e-153
Identity = 278/337 (82.49%), Postives = 296/337 (87.83%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ ACH FGFFKLINHG+S HAI+ LE
Sbjct: 1 MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK- 120
AFDFFAKTAPEK RA PA PFGYG KAIGPNGD G+LEYLLLRSD AS+ ++S SIS
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
PS F YVVNEYVKSV++L CEILDLVGEGLWLPNKSVF++LIRDV SDSVL I
Sbjct: 121 PSTF-------SYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRI 180
Query: 181 NYYPAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPV 240
NYYPAVKHTENWEL SSKS+PS N+IGFGEHS+PQILTILRSNDVDGLQISLQDGLW PV
Sbjct: 181 NYYPAVKHTENWEL-SSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPV 240
Query: 241 CPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM 300
CP PSA YVLIGDA QAMTNGRFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAPLSEM
Sbjct: 241 CPDPSAFYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEM 300
Query: 301 MLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
+LPN +SLYKPFTWAQYKK AYSLRLADSRLDLFKAS
Sbjct: 301 ILPNHQSLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Spg025690 vs. NCBI nr
Match:
XP_023547502.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014296.1 Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 550.4 bits (1417), Expect = 1.0e-152
Identity = 278/337 (82.49%), Postives = 296/337 (87.83%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ ACH FGFFKLINHG+S HAI+ LE
Sbjct: 1 MPCPTAMRTKKTKVVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK- 120
AFDFFAKTAPEK RA PA PFGYG KAIGPNGD G+LEYLLLRSD AS+ ++S SIS
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
PS F YVVNEYVKSV++L CEILDLVGEGLWLPNKSVF++LIRDV SDSVL I
Sbjct: 121 PSTF-------SYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRI 180
Query: 181 NYYPAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPV 240
NYYPAVKHTENWEL SSKS+PS N+IGFGEHS+PQILTILRSNDVDGLQISLQDGLW PV
Sbjct: 181 NYYPAVKHTENWEL-SSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPV 240
Query: 241 CPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM 300
CP PSA YVLIGDA QAMTNGRFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAPLSEM
Sbjct: 241 CPDPSAFYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEM 300
Query: 301 MLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
+LPN +SLYKPFTWAQYKK AYSLRLADSRLDLFKAS
Sbjct: 301 ILPNHQSLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Spg025690 vs. NCBI nr
Match:
KAG7025608.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 549.3 bits (1414), Expect = 2.3e-152
Identity = 282/338 (83.43%), Postives = 297/338 (87.87%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC FG FK+INHG+S HAIA LE
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASIS-K 120
QAFDFF +TAPEKRRA PA PFGYGCKAIGPNGD+G+LEY+LLRSD ASIAE+SASIS
Sbjct: 61 QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
P F YVVNEYVKSVRELACEILDLVGEGLWLPNKSVF+NLIRDVQSDSVL I
Sbjct: 121 PLGF-------SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRI 180
Query: 181 NYYP-AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVP 240
NYYP AVKHTE +SSKSH STNKIGFGEHS+PQILTILRSNDVDGLQISL DGLW P
Sbjct: 181 NYYPAAVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTP 240
Query: 241 VCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSE 300
VCP PSA YVLIGDAFQAMTN RFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAP S+
Sbjct: 241 VCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSD 300
Query: 301 MMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
MMLP ++SLYKPFTWAQYKK AYSLRLADSRL+LFKAS
Sbjct: 301 MMLPKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Spg025690 vs. NCBI nr
Match:
KAG6593256.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 549.3 bits (1414), Expect = 2.3e-152
Identity = 282/338 (83.43%), Postives = 297/338 (87.87%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC FG FK+INHG+S HAIA LEH
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLEH 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASIS-K 120
QAFDFF +TAPEKRRA PA PFGYGCKAIGPNGD+G+LEY+LLRSD ASIAE+SASIS
Sbjct: 61 QAFDFFGQTAPEKRRAGPATPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
P F YVVNEYVKSVRELACEILDLVGEGLWLPNKSVF+NLIRDVQSDSVL I
Sbjct: 121 PLGF-------SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRI 180
Query: 181 NYYP-AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVP 240
NYYP AVKHTE +SSKSH STNKIGFGEHS+PQILTILRSNDVDGLQISL DGLW P
Sbjct: 181 NYYPAAVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTP 240
Query: 241 VCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSE 300
VCP PSA YVLIGDAFQAMTN RFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAP S+
Sbjct: 241 VCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSD 300
Query: 301 MMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
MML ++SLYKPFTWAQYKK AYSLRLADSRL+LFKAS
Sbjct: 301 MMLTKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Spg025690 vs. NCBI nr
Match:
XP_023514766.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 546.2 bits (1406), Expect = 2.0e-151
Identity = 280/338 (82.84%), Postives = 296/338 (87.57%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC FG FK+INHG+S HAIA LE
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGIFKVINHGVSDHAIANLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASIS-K 120
QAFDFF +TAPEK RA PA PFGYGCKAIGPNGD+G+LEY+LLRSD ASIAE+SASIS
Sbjct: 61 QAFDFFGQTAPEKHRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
P F YVVNEYVKSVRELACEILDLVGEGLWLPNKSVF+NLIRDVQSDSVL I
Sbjct: 121 PLGF-------SYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRI 180
Query: 181 NYYP-AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVP 240
NYYP AVKHTE +SSKSH STNK+GFGEHS+PQILTILRSNDVDGLQISL DGLW P
Sbjct: 181 NYYPAAVKHTEKSRELSSKSHLSTNKVGFGEHSDPQILTILRSNDVDGLQISLHDGLWTP 240
Query: 241 VCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSE 300
VCP PSA YVLIGDAFQAMTN RFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAP S+
Sbjct: 241 VCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSD 300
Query: 301 MMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
MMLP ++SLYKPFTWAQYKK AYSLRLADSRL+LFKAS
Sbjct: 301 MMLPKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Spg025690 vs. ExPASy Swiss-Prot
Match:
Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)
HSP 1 Score: 377.9 bits (969), Expect = 1.2e-103
Identity = 188/337 (55.79%), Postives = 247/337 (73.29%), Query Frame = 0
Query: 1 MPCPTAM-RTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLE 60
+P PT+M RTKKTK VGIPTIDLS R++ S+ +V+AC ++GFFK++NH + I+ L+
Sbjct: 3 VPSPTSMIRTKKTKAVGIPTIDLSLERSQLSELVVKACEEYGFFKVVNHSVPKEVISRLD 62
Query: 61 HQAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK 120
+ +FF+K + EKR+A + PFGYGCK IGPNGD G LEYLLL S+ SI+E S +I+K
Sbjct: 63 EEGIEFFSKNSSEKRQAGTSTPFGYGCKNIGPNGDKGELEYLLLHSNPISISERSKTIAK 122
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
+ +VN+Y+K+V++L CEIL+L EGLW+P+KS + +I+D SDS+L I
Sbjct: 123 DHPIK-----FSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKIIKDEHSDSLLRI 182
Query: 181 NYYPAVKH--TENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWV 240
N+YP VK +NW+ S + + N IGFGEHS+PQILTILRSN+V GLQIS GLW+
Sbjct: 183 NHYPPVKKLGNDNWD-PSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGLQISTHHGLWI 242
Query: 241 PVCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLS 300
PV P PS YV++GDA Q +TNGRFVSVRHR + N + KPRMS MYF APPLN I+PLS
Sbjct: 243 PVPPDPSEFYVMVGDALQVLTNGRFVSVRHRVLTNTT-KPRMSMMYFAAPPLNWLISPLS 302
Query: 301 EMMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFK 335
+M+ + LY+PFTWAQYK+ AY+LRL D+RLD FK
Sbjct: 303 KMVTAHSPCLYRPFTWAQYKQAAYALRLGDTRLDQFK 332
BLAST of Spg025690 vs. ExPASy Swiss-Prot
Match:
Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)
HSP 1 Score: 322.8 bits (826), Expect = 4.7e-87
Identity = 163/331 (49.24%), Postives = 213/331 (64.35%), Query Frame = 0
Query: 5 TAMRTKKTKPVGIPTIDLS-ESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAF 64
T +T + P ID S R++ S+ IV+AC GFFK+INHG+ I EH+
Sbjct: 12 TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71
Query: 65 DFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISKPSYF 124
+FF K +K RA PA+PFGYGCK IG NGD+G LEYLLL ++ ++A+ S +IS F
Sbjct: 72 EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131
Query: 125 QSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYP 184
+ N+Y+++VR+LACEI+DL E LW S + LIRDV+SDS+L +N+YP
Sbjct: 132 K-----FSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYP 191
Query: 185 AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYP 244
+ + +IGFGEHS+PQILT+LRSNDVDGL+I +DGLW+P+ P
Sbjct: 192 PAPYALS----------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDP 251
Query: 245 SALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLPN 304
+ +VL+GD QA+TNGRF SVRHR +AN +KKPRMS MYF APPL A I+PL +M+ P
Sbjct: 252 TCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPE 311
Query: 305 EKSLYKPFTWAQYKKVAYSLRLADSRLDLFK 335
Y FTW YKK YSLRL RL+ FK
Sbjct: 312 NPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327
BLAST of Spg025690 vs. ExPASy Swiss-Prot
Match:
Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)
HSP 1 Score: 283.9 bits (725), Expect = 2.4e-75
Identity = 158/320 (49.38%), Postives = 205/320 (64.06%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFDFFAKTAPEKRRA 76
IP ID+S+ R++ S IV+AC GFFK+INHG+ I+ +E ++ +FFAK A EK+
Sbjct: 15 IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74
Query: 77 APAN-PFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISKPSYFQSGIFIVGYVVN 136
P N PF YG + IG NGD G +EYLL ++ + S S S VN
Sbjct: 75 RPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPAF-RSQLSFSS-------------AVN 134
Query: 137 EYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYPAVKHTENWELIS 196
Y+++V++LA EILDL EGL +P S F+ LI V SDSVL +N+YP +S
Sbjct: 135 CYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLS 194
Query: 197 SKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYPSALYVLIGDAFQ 256
+S S ++GFGEH++PQILT+LRSN V GLQ+S DG+WV V P PSA V +GD Q
Sbjct: 195 DQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQ 254
Query: 257 AMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM-MLPNEKSLYKPFTWA 316
MTNGRF+SVRHRA+ ++ R+ST YF PPL A I PLS M M N+ LY+ FTW
Sbjct: 255 VMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWG 314
Query: 317 QYKKVAYSLRLADSRLDLFK 335
+YKK AYSLRL DSRLD+F+
Sbjct: 315 EYKKRAYSLRLEDSRLDMFR 317
BLAST of Spg025690 vs. ExPASy Swiss-Prot
Match:
Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)
HSP 1 Score: 280.0 bits (715), Expect = 3.5e-74
Identity = 145/323 (44.89%), Postives = 205/323 (63.47%), Query Frame = 0
Query: 16 GIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFDFFAKTAPEKRR 75
GIP +DL+ A+ IV AC DFGFFKL+NHG+ +A LE++A FF K+ EK R
Sbjct: 26 GIPVVDLTHPDAK--NLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEKDR 85
Query: 76 AAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASI--SKPSYFQSGIFIVGYV 135
A P +PFGYG K IGPNGD+G +EYLLL ++ I+ S I P +F++ V
Sbjct: 86 AGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENPHHFRA-------V 145
Query: 136 VNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYPAVKHTENWEL 195
V Y+ +V+ + +L+L+ EGL + ++ + L++D +SDS +N+YP +
Sbjct: 146 VENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPEVQAL-- 205
Query: 196 ISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYPSALYVLIGDA 255
+ N +GFGEH++PQI+++LRSN GLQI L DG WV V P ++ ++ +GDA
Sbjct: 206 -------NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDA 265
Query: 256 FQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLPNEKSLYKPFTW 315
Q MTNGRF SV+HR +A+ +K R+S +YFG P L+ IAPL +ML E+ LYK FTW
Sbjct: 266 LQVMTNGRFKSVKHRVLADTTKS-RLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTW 325
Query: 316 AQYKKVAYSLRLADSRLDLFKAS 337
+YKK AY+ RLAD+RL F+ S
Sbjct: 326 CEYKKAAYTSRLADNRLAPFQKS 329
BLAST of Spg025690 vs. ExPASy Swiss-Prot
Match:
Q5ZA21 (Gibberellin 2-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX2 PE=3 SV=1)
HSP 1 Score: 277.3 bits (708), Expect = 2.2e-73
Identity = 146/340 (42.94%), Postives = 210/340 (61.76%), Query Frame = 0
Query: 6 AMRTKKTKPVGIPTIDLS--ESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAF 65
A+ ++ + V +PT+D+S R S+ + AC GFF+ +NHG+ A ++ A
Sbjct: 23 AVFCRRGRGVVVPTVDMSAPAGRGELSRQVARACAGSGFFRAVNHGVPPRVSAAMDAAAA 82
Query: 66 DFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK--PS 125
FF + EK+ A P +P GYG ++IG NGD+G LEYL+L + ++A +++I + P
Sbjct: 83 AFFVRAGAEKQLAGPPDPLGYGSRSIGANGDVGELEYLILHASPDAVARKASAIDREDPR 142
Query: 126 YFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINY 185
F VVN+YV++VR+LAC +LDL+GEGL L + + T LI +DS++ IN+
Sbjct: 143 RFSQ-------VVNDYVEAVRQLACHVLDLLGEGLGLRDPTSLTRLITATDNDSLIRINH 202
Query: 186 YPAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQ------DGL 245
YP + S T IGFGEH++PQIL++LR+ND DGLQ+ L D +
Sbjct: 203 YPPSCAAAAGDHKSGGGPAPTAAIGFGEHTDPQILSVLRANDADGLQLLLPDAAAAGDSV 262
Query: 246 WVPVCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAP 305
WVPV P PSA +V +GD QA+TNGR VS+RHR + KPR+ST+YF APPL+A I+
Sbjct: 263 WVPVPPDPSAFFVNVGDLLQALTNGRLVSIRHRVVVGTG-KPRLSTIYFAAPPLHARISA 322
Query: 306 LSEMMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKA 336
L E + Y+ FTWA+YK+ Y+LRL+ +RLDLF A
Sbjct: 323 LPETVAAGAPRRYRAFTWAEYKRTMYTLRLSHNRLDLFHA 354
BLAST of Spg025690 vs. ExPASy TrEMBL
Match:
A0A6J1GRC3 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456420 PE=3 SV=1)
HSP 1 Score: 550.8 bits (1418), Expect = 3.9e-153
Identity = 278/337 (82.49%), Postives = 296/337 (87.83%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MPCPTAMRTKKTK VGIPTIDLS++R TSKSI+ ACH FGFFKLINHG+S HAI+ LE
Sbjct: 1 MPCPTAMRTKKTKAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK- 120
AFDFFAKTAPEK RA PA PFGYG KAIGPNGD G+LEYLLLRSD AS+ ++S SIS
Sbjct: 61 HAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
PS F YVVNEYVKSV++L CEILDLVGEGLWLPNKSVF++LIRDV SDSVL I
Sbjct: 121 PSTF-------SYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRI 180
Query: 181 NYYPAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPV 240
NYYPAVKHTENWEL SSKS+PS N+IGFGEHS+PQILTILRSNDVDGLQISLQDGLW PV
Sbjct: 181 NYYPAVKHTENWEL-SSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPV 240
Query: 241 CPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM 300
CP PSA YVLIGDA QAMTNGRFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAPLSEM
Sbjct: 241 CPDPSAFYVLIGDALQAMTNGRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPLSEM 300
Query: 301 MLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
+LPN +SLYKPFTWAQYKK AYSLRLADSRLDLFKAS
Sbjct: 301 ILPNHQSLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Spg025690 vs. ExPASy TrEMBL
Match:
A0A6J1JXB5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488342 PE=3 SV=1)
HSP 1 Score: 545.4 bits (1404), Expect = 1.6e-151
Identity = 276/337 (81.90%), Postives = 296/337 (87.83%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MPCPTAMRTKKT+ VGIPTIDLS++R TSKSI+ ACH FGFFKLINHG+S HAI+ LE
Sbjct: 1 MPCPTAMRTKKTEAVGIPTIDLSQTRPTTSKSILHACHHFGFFKLINHGVSHHAISRLEC 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK- 120
QAFDFFAKTAPEK RA PA PFGYG KAIGPNGD G+LEYLLLRSD AS+ ++S SIS
Sbjct: 61 QAFDFFAKTAPEKHRAGPAAPFGYGTKAIGPNGDTGDLEYLLLRSDPASVLQTSPSISND 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
PS F YVVNEYVKSV++L CEILDLVGEGLWLPNKSVF++LIRDV SDSVL I
Sbjct: 121 PSTF-------SYVVNEYVKSVKDLGCEILDLVGEGLWLPNKSVFSDLIRDVHSDSVLRI 180
Query: 181 NYYPAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPV 240
NYYPAVKHTENWEL SSKS+PS N+IGFGEHS+PQILTILRSNDVDGLQISLQDGLW PV
Sbjct: 181 NYYPAVKHTENWEL-SSKSNPSNNRIGFGEHSDPQILTILRSNDVDGLQISLQDGLWRPV 240
Query: 241 CPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM 300
CP PSA YVLIGDA QAMTNGRFVSVRHRAMAN+SKK RMS MYFGAPPLNAWIAPLSEM
Sbjct: 241 CPDPSAFYVLIGDALQAMTNGRFVSVRHRAMANSSKKARMSIMYFGAPPLNAWIAPLSEM 300
Query: 301 MLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
+ PN +SLYKPFTWAQYKK AYSLRLADSRLDLFKAS
Sbjct: 301 IPPNHQSLYKPFTWAQYKKAAYSLRLADSRLDLFKAS 329
BLAST of Spg025690 vs. ExPASy TrEMBL
Match:
A0A6J1DEJ9 (gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 PE=3 SV=1)
HSP 1 Score: 545.0 bits (1403), Expect = 2.1e-151
Identity = 274/339 (80.83%), Postives = 295/339 (87.02%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MPCPTAMRTKK K VGIPTIDLSESRARTSK+IVEAC +FGFFKL+NHG+S AIAGLE
Sbjct: 1 MPCPTAMRTKKAKAVGIPTIDLSESRARTSKAIVEACEEFGFFKLVNHGVSDQAIAGLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK- 120
Q FDFFAK APEKRRA PA+PFGYGCK IGPNGD G LEYLLLRSD A+IAE+S SIS
Sbjct: 61 QGFDFFAKPAPEKRRAGPADPFGYGCKTIGPNGDTGELEYLLLRSDPAAIAETSLSISSD 120
Query: 121 -PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLS 180
PS+ Y VNEY+KSV+ELACEILDLVGEGLWLPNKS+ + LIRDVQSDSVL
Sbjct: 121 PPSF--------SYAVNEYIKSVKELACEILDLVGEGLWLPNKSIISGLIRDVQSDSVLR 180
Query: 181 INYYPAVKHTENWELISSKSHPSTN-KIGFGEHSNPQILTILRSNDVDGLQISLQDGLWV 240
IN+YPAVKHTE+WEL SSKSHPS N +IGFGEHS+PQILTILRSNDV GLQISL GLWV
Sbjct: 181 INHYPAVKHTEDWEL-SSKSHPSNNTRIGFGEHSDPQILTILRSNDVGGLQISLPGGLWV 240
Query: 241 PVCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLS 300
PVCP PSA YVLIGDAFQAM+NGRFVSVRHRAMANNS KPRMS MYFGAPPLNAWIAPLS
Sbjct: 241 PVCPDPSAFYVLIGDAFQAMSNGRFVSVRHRAMANNSNKPRMSMMYFGAPPLNAWIAPLS 300
Query: 301 EMMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
E+MLP+ S+Y+PFTW QYKK AYSLRLADSRLDLFKAS
Sbjct: 301 ELMLPHHPSMYQPFTWGQYKKAAYSLRLADSRLDLFKAS 330
BLAST of Spg025690 vs. ExPASy TrEMBL
Match:
A0A6J1H8N0 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460653 PE=3 SV=1)
HSP 1 Score: 543.5 bits (1399), Expect = 6.2e-151
Identity = 280/338 (82.84%), Postives = 295/338 (87.28%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MP PTAMR+KKTK VGIPTIDLSESRARTSKSIVEAC FG FK+INHG+S HAIA LE
Sbjct: 1 MPFPTAMRSKKTKAVGIPTIDLSESRARTSKSIVEACEHFGIFKVINHGVSDHAIANLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASIS-K 120
QAFDFF +TAPEKRRA PA PFGYGCKAIGPNGD+G+LEY+LLRSD ASIAE+SASIS
Sbjct: 61 QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLRSDPASIAETSASISNS 120
Query: 121 PSYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSI 180
P F YVVNEYVK VRELACEILDLVGEGLWLPNKSVF+NLIRDVQSDSVL I
Sbjct: 121 PLGF-------SYVVNEYVKCVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRI 180
Query: 181 NYYP-AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVP 240
NYYP AVKHTE +SSKSH STNKIGFGEHS+PQILTILRSNDVDGLQISL DGLW P
Sbjct: 181 NYYPAAVKHTEKSRELSSKSHLSTNKIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTP 240
Query: 241 VCPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSE 300
VCP PSA YVLIGDAFQAMTN RFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAP S+
Sbjct: 241 VCPDPSAFYVLIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSD 300
Query: 301 MMLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
MML ++SLYKPFTWAQYKK AYSLRLADSRL+LFKAS
Sbjct: 301 MMLTKDQSLYKPFTWAQYKKAAYSLRLADSRLNLFKAS 331
BLAST of Spg025690 vs. ExPASy TrEMBL
Match:
A0A6J1KWQ4 (gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=3 SV=1)
HSP 1 Score: 543.1 bits (1398), Expect = 8.1e-151
Identity = 277/337 (82.20%), Postives = 293/337 (86.94%), Query Frame = 0
Query: 1 MPCPTAMRTKKTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEH 60
MP PTA R+KKTK VGIPTIDLSESRARTSKSIVEAC FGFFK+INHG+S HAIA LE
Sbjct: 1 MPFPTATRSKKTKAVGIPTIDLSESRARTSKSIVEACQHFGFFKVINHGVSDHAIANLER 60
Query: 61 QAFDFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISKP 120
QAFDFF +TAPEKRRA PA PFGYGCKAIGPNGD+G+LEY+LL SD ASIAE+SASIS
Sbjct: 61 QAFDFFGQTAPEKRRAGPAAPFGYGCKAIGPNGDIGDLEYILLHSDPASIAETSASIS-- 120
Query: 121 SYFQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSIN 180
+ YVVNEYVKSVRELACEILDLVGEGLWLPNKSVF+NLIRDVQSDSVL IN
Sbjct: 121 ----NSPLAFSYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFSNLIRDVQSDSVLRIN 180
Query: 181 YYP-AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPV 240
YYP AVKHTE +SSKSH STN IGFGEHS+PQILTILRSNDVDGLQISL DGLW PV
Sbjct: 181 YYPAAVKHTEKSRELSSKSHLSTNNIGFGEHSDPQILTILRSNDVDGLQISLHDGLWTPV 240
Query: 241 CPYPSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM 300
CP PSA Y+LIGDAFQAMTN RFVSVRHRAMANNSKK RMS MYFGAPPLNAWIAP S+M
Sbjct: 241 CPDPSAFYILIGDAFQAMTNDRFVSVRHRAMANNSKKARMSIMYFGAPPLNAWIAPPSDM 300
Query: 301 MLPNEKSLYKPFTWAQYKKVAYSLRLADSRLDLFKAS 337
MLP ++SLYKPFTWAQYKK AYSLRLADSRL LFKAS
Sbjct: 301 MLPKDQSLYKPFTWAQYKKAAYSLRLADSRLHLFKAS 331
BLAST of Spg025690 vs. TAIR 10
Match:
AT1G02400.1 (gibberellin 2-oxidase 6 )
HSP 1 Score: 322.8 bits (826), Expect = 3.3e-88
Identity = 163/331 (49.24%), Postives = 213/331 (64.35%), Query Frame = 0
Query: 5 TAMRTKKTKPVGIPTIDLS-ESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAF 64
T +T + P ID S R++ S+ IV+AC GFFK+INHG+ I EH+
Sbjct: 12 TGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGE 71
Query: 65 DFFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISKPSYF 124
+FF K +K RA PA+PFGYGCK IG NGD+G LEYLLL ++ ++A+ S +IS F
Sbjct: 72 EFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPF 131
Query: 125 QSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYP 184
+ N+Y+++VR+LACEI+DL E LW S + LIRDV+SDS+L +N+YP
Sbjct: 132 K-----FSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYP 191
Query: 185 AVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYP 244
+ + +IGFGEHS+PQILT+LRSNDVDGL+I +DGLW+P+ P
Sbjct: 192 PAPYALS----------GVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDP 251
Query: 245 SALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLPN 304
+ +VL+GD QA+TNGRF SVRHR +AN +KKPRMS MYF APPL A I+PL +M+ P
Sbjct: 252 TCFFVLVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPE 311
Query: 305 EKSLYKPFTWAQYKKVAYSLRLADSRLDLFK 335
Y FTW YKK YSLRL RL+ FK
Sbjct: 312 NPRRYNSFTWGDYKKATYSLRLDVPRLEFFK 327
BLAST of Spg025690 vs. TAIR 10
Match:
AT1G47990.1 (gibberellin 2-oxidase 4 )
HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 158/320 (49.38%), Postives = 205/320 (64.06%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFDFFAKTAPEKRRA 76
IP ID+S+ R++ S IV+AC GFFK+INHG+ I+ +E ++ +FFAK A EK+
Sbjct: 15 IPIIDMSQERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEKKSV 74
Query: 77 APAN-PFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISKPSYFQSGIFIVGYVVN 136
P N PF YG + IG NGD G +EYLL ++ + S S S VN
Sbjct: 75 RPVNQPFRYGFRDIGLNGDSGEVEYLLFHTNDPAF-RSQLSFSS-------------AVN 134
Query: 137 EYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYPAVKHTENWELIS 196
Y+++V++LA EILDL EGL +P S F+ LI V SDSVL +N+YP +S
Sbjct: 135 CYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQFFGEANLS 194
Query: 197 SKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYPSALYVLIGDAFQ 256
+S S ++GFGEH++PQILT+LRSN V GLQ+S DG+WV V P PSA V +GD Q
Sbjct: 195 DQS-VSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCVNVGDLLQ 254
Query: 257 AMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEM-MLPNEKSLYKPFTWA 316
MTNGRF+SVRHRA+ ++ R+ST YF PPL A I PLS M M N+ LY+ FTW
Sbjct: 255 VMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRLYQTFTWG 314
Query: 317 QYKKVAYSLRLADSRLDLFK 335
+YKK AYSLRL DSRLD+F+
Sbjct: 315 EYKKRAYSLRLEDSRLDMFR 317
BLAST of Spg025690 vs. TAIR 10
Match:
AT2G34555.1 (gibberellin 2-oxidase 3 )
HSP 1 Score: 272.3 bits (695), Expect = 5.1e-73
Identity = 143/326 (43.87%), Postives = 208/326 (63.80%), Query Frame = 0
Query: 11 KTKPVGIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFDFFAKTA 70
K +PV IP IDL++S A+T IV+AC +FGFFK+INHG+ + LE +A +FFA
Sbjct: 21 KPRPVLIPVIDLTDSDAKT--QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHH 80
Query: 71 PEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSDLASIAESSASISK--PSYFQSGIF 130
K +A P +PFGYG K IGPNGD+G LEY+LL ++L + + +I + P+ F+
Sbjct: 81 SLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFRE--- 140
Query: 131 IVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYPAVKHT 190
V EY+K ++ ++ + L++V E L + K + L++ +SDS L +N+YP + T
Sbjct: 141 ----AVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEET 200
Query: 191 ENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYPSALYV 250
P +IGFGEH++PQ++++LRSND +GLQI ++DG WV V P S+ +V
Sbjct: 201 -----------PVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFV 260
Query: 251 LIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLPNEKSLY 310
L+GD Q MTNGRF SV+HR + N+K+ R+S +YF PPL+ IAPLS ++ + LY
Sbjct: 261 LVGDTLQVMTNGRFKSVKHR-VVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLY 320
Query: 311 KPFTWAQYKKVAYSLRLADSRLDLFK 335
FTW+QYK AY +L D RL LF+
Sbjct: 321 NEFTWSQYKLSAYKTKLGDYRLGLFE 325
BLAST of Spg025690 vs. TAIR 10
Match:
AT1G30040.1 (gibberellin 2-oxidase )
HSP 1 Score: 261.2 bits (666), Expect = 1.2e-69
Identity = 140/332 (42.17%), Postives = 209/332 (62.95%), Query Frame = 0
Query: 9 TKKTKPV----GIPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFD 68
T K PV IP ++L++ A+T IV+AC +FGFFK++NHG+ + LE +A
Sbjct: 19 TYKPVPVLTSHSIPVVNLADPEAKT--RIVKACEEFGFFKVVNHGVRPELMTRLEQEAIG 78
Query: 69 FFAKTAPEKRRAAPANPFGYGCKAIGPNGDMGNLEYLLLRSD--LASIAESSASISKPSY 128
FF K RA P P+GYG K IGPNGD+G +EYLLL ++ L+S S+ P
Sbjct: 79 FFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFRQTPQI 138
Query: 129 FQSGIFIVGYVVNEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYY 188
F+ V EY+K ++E++ ++L++V E L + + + ++RD +SDS L +N+Y
Sbjct: 139 FRES-------VEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHY 198
Query: 189 PAVKHTENWELISSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPY 248
PA + E +++ K+GFGEH++PQI+++LRSN+ GLQI ++DG WV V P
Sbjct: 199 PAAEE-EAEKMV---------KVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPD 258
Query: 249 PSALYVLIGDAFQAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLP 308
S+ ++ +GDA Q MTNGRF SV+HR +A ++++ R+S +YFG PPL+ IAPL ++
Sbjct: 259 HSSFFINVGDALQVMTNGRFKSVKHRVLA-DTRRSRISMIYFGGPPLSQKIAPLPCLVPE 318
Query: 309 NEKSLYKPFTWAQYKKVAYSLRLADSRLDLFK 335
+ LYK FTW+QYK AY +L D RL LF+
Sbjct: 319 QDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFE 330
BLAST of Spg025690 vs. TAIR 10
Match:
AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )
HSP 1 Score: 252.7 bits (644), Expect = 4.2e-67
Identity = 132/320 (41.25%), Postives = 202/320 (63.12%), Query Frame = 0
Query: 17 IPTIDLSESRARTSKSIVEACHDFGFFKLINHGLSAHAIAGLEHQAFDFFAKTAPEKRRA 76
IP ID+S+ ++ ++V+AC DFGFFK+INHG+SA ++ LEH+ DFF+ EK +
Sbjct: 18 IPVIDMSDPESK--HALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQV 77
Query: 77 APANPFGYGCKAIGPNGDMGNLEYLLLRS--DLASIAESSASISKPSYFQSGIFIVGYVV 136
A PFGYG IG NGD+G +EYLL+ + D S + + P F++ +
Sbjct: 78 A-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNAL------- 137
Query: 137 NEYVKSVRELACEILDLVGEGLWLPNKSVFTNLIRDVQSDSVLSINYYPAVKHTENWELI 196
EY SVR++ ++L+ + +GL + ++ + L+ D +DS+L +N+YP L
Sbjct: 138 EEYTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPC------PLS 197
Query: 197 SSKSHPSTNKIGFGEHSNPQILTILRSNDVDGLQISLQDGLWVPVCPYPSALYVLIGDAF 256
+ K++ N IGFGEH++PQI+++LRSN+ GLQI+L DG W+ V P ++ + +GD+
Sbjct: 198 NKKTNGGKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSL 257
Query: 257 QAMTNGRFVSVRHRAMANNSKKPRMSTMYFGAPPLNAWIAPLSEMMLPNEKSLYKPFTWA 316
Q MTNGRF SVRHR +A N KK R+S +YF P L IAPL+ ++ ++ LY+ FTW+
Sbjct: 258 QVMTNGRFKSVRHRVLA-NCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWS 317
Query: 317 QYKKVAYSLRLADSRLDLFK 335
+YK Y+ RL+D+RL F+
Sbjct: 318 EYKNSTYNSRLSDNRLQQFE 320
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022954040.1 | 8.0e-153 | 82.49 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata] | [more] |
XP_023547502.1 | 1.0e-152 | 82.49 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] >KAG6575745.1... | [more] |
KAG7025608.1 | 2.3e-152 | 83.43 | Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyros... | [more] |
KAG6593256.1 | 2.3e-152 | 83.43 | Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_023514766.1 | 2.0e-151 | 82.84 | gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q9XHM5 | 1.2e-103 | 55.79 | Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1 | [more] |
Q9FZ21 | 4.7e-87 | 49.24 | Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ... | [more] |
Q9C7Z1 | 2.4e-75 | 49.38 | Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ... | [more] |
Q9XG83 | 3.5e-74 | 44.89 | Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... | [more] |
Q5ZA21 | 2.2e-73 | 42.94 | Gibberellin 2-beta-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GRC3 | 3.9e-153 | 82.49 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111456... | [more] |
A0A6J1JXB5 | 1.6e-151 | 81.90 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148834... | [more] |
A0A6J1DEJ9 | 2.1e-151 | 80.83 | gibberellin 2-beta-dioxygenase 2 OS=Momordica charantia OX=3673 GN=LOC111019794 ... | [more] |
A0A6J1H8N0 | 6.2e-151 | 82.84 | gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460... | [more] |
A0A6J1KWQ4 | 8.1e-151 | 82.20 | gibberellin 2-beta-dioxygenase 2 OS=Cucurbita maxima OX=3661 GN=LOC111497847 PE=... | [more] |