Spg025427 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025427
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionZinc transporter 4
Locationscaffold13: 40808162 .. 40811724 (-)
RNA-Seq ExpressionSpg025427
SyntenySpg025427
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCAAAAAGTCATAACTGTGGTTTATCCCTCTGCGTCTGCCCTCCATTTCTTCCATTTATGTCGACACTCTTCTTCTCTATTCCCCTTCTTCTCTTCCTTCCCACTTCCTTCTTCCTTCTGTCTCCTTCTTTTTCTTCTCCTTTTTCTCTCTTTTTTGCTTCTCCGATCGCTCGCTGCGGCTGCCTTTTTATAAAAACCGAAACAAAACCCCTTCTCTCCACCGCCGCCCCCATCCATCCTTCTCCTCCGTTTCTCCAACATTCCAGATTTGGCTTCTTCGCATCTCATGTTCTTCTTCGAGGTTCCAACCCGATCCTTTTTCTCTTCGGTTTCTGGGTATTCCGAATTTGCCTTTGGCTGATGGGTTTCTTTTCTCTTTCAGGATTTCTGGCAGATGCTCTGTATTCAAGGCTTCCGGGATAGAACTCGACTTCTCTCTGGTAATTTGAATTTCCTTTTCTCCGATTCCATTGGACGAGTTTGTTTTCGATTGAATTTTTCTTCCTTGTGTCTGTTCCGCTCTGAATTGAACTGGGTCAATCGTTGAATTTTCTGGGTTCCTAATTTGATGCACTCTCTGACTGGTTGCTGAGAGTTTCTGAATTCTTGTGATTCGTTGGTTCTTGATTTCTAGGTTTTAGTAATACTGGGTTCCTTTTGAAATTTTGGTGAATGACTTTTCGTTTTTCAATTTCCAATCGAACAGAGTCCCTTATACGGACCGTATCCGAATCCATGGGCAATTCGTCCTGTGGCGCCTCCGAGTTAGAACTCTGTCGAAACGACTCCGCTGCGCTTAATTTCAAATTAATCGCAATCGCCTCGATTCTCACATCGGGAGTCGTCGGAATCGTCATTCCGTTGTTCGGCAGACATCGCCGGTTCCTCAAGACCGACGGCAACCTGTTCGTGGCGGCGAAGGCTTTCGCCGCGGGGGTGATTCTGGCCACCGCGTTCGTCCACATGCTCCCCGACGGATCGAAAGCTCTGTCCGATCCGTGCTTGCCGGAATTCCCCTGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCTTCGCTGCTGACGCTCCTCGTCGATTTCGTCGGAACTCAGTACTACGAGCGGAAACAGGGGCTGATTCGATCGAAAGAGGAAGCTAGTCGGGTCGGGTCGGAGGTGGATTCCGGATTGGAATCCGGCATCCTCCCGGCCGCCGATTCGAACGCGAAAGTGTTCGGCGAAGAGGACGGCGGCGCGATGCACATCGTCGGAATCCATGCGCACGCGGCGCACCACCGGCACAGTCATCCGCAGGGGAAGGAGGCGTGTGACGGGCATGTGACACTCCATGACCATGGCCATGCCCATGGACATGGGCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGGTATCTCCATCCATCTATTTTTCTCTATTTTTATTGTTTTTTTTTTAAACACTTTCAAAATGTCAATGTCGTTGAACCGATAAACCAAACTCTTTCCATCAATTGTTGTTTTTTTGTTTTCTCTCTTTTTCTTTTTTATTTCCTTATGAGGAACGGCAATATTCTGCTCACTGAAGTCAACAAACCATAATTTGTTTCAAATAGTTTTTTACGGATTAAATTATAAATTTTGTTTTTTTAAAAAAAAAAATAAAAAGTGATTTTTGTGATGGGTTCCTAAACTATTTACTCATGTTTGGTAAATTCTTTTTTTTTTTCTTTGACATTATGTTTGGTAAATTCTTGAGTTTACCAAAAATGGGAAATTTAAACATCTACGAAATTGAAAGTTCAAACACCAGTCTACTAAGGGATAATCACGAGGTCTGTGGTTCGAATCCCTATCTCCCAATTGTCAAAAAAAAAAAAAAAAAAAAAAAGTTAGCTTGTTCTAGAAATCTATTTATTAGACACATTTTAAGGTTTGGTTAGTGATTGGATAGGTATAAAAAGTTAAATTACAAGTTTAATTCTTGCAACTTTGGTTGCGTCTATTTGATTCTTAACTTTTAGGATTGTGTTAATTTGATTTCGGAACCCTAAGTGTCTAATAGGTCTTTGAATTATTAATTTTGTATCTAATATGTTCTTGAATTTAATTTCTAATAGGTTCTTGAATTTAATTTTTAAAAAATAATTAATCTAATAGATATAATTTTATGTTTATCGGGACCCTAAACTTCCATATCTAGTCGTTTATGAATTTTTTAAAATATCGGATGTGTCAAAGACTTTAGACACAAAAGTGAAAGTTTAAAGCCTACGAGATACTAGATATTTTTTAAAGAAAAGTTTGTCCCATTAATTGAACCTTATAAAAACTTACCTCAATCATAGACTTTTTATTTTTTAATTGAGGGGTCTTCTAAACACAATTTTAAAAGTTTAGGACCTATAATTTAATTTTTATTTTATAGAACATAAGATGAGTTTATAAACTCAAGATGTACTTTAGTTTTTTATTAGTTTTTGATGTCCTTTTCTAAATTGGAACTATTTATTCGTTTCATTTTTTTTATTTTTTTTTTTATATATTCTTAATAGCGAAATTTAAAAAGTTTCTGAGCTTGCAAATGCGAGGATTCCATGGGAGAGCCAAATTTTGAAATGCTTTATTATTATTATTATTATTATTATTATTATTCAGGAATCATTGTTTATTTTCCTTTTCTTGAAAAAAAAATGTTGTTGTCCATATTTTAATAATTGTTTTGTAATTTCAGTTCAAAATTTAAAAAGGTAAGTAGGGTCGATTTGATGACCGTATATAACTTACCATGTATGGAAAATAATACACTAGAAGAATATATAATATAAAAGGGCAATTTTGAAAAAGAAAAATCCCAATTATTGATTTATCTAAAAATATTTTTATATTTGGGCAGATTTTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCCTTAATTGCAGCCCTCTCCTTCCATCAGTTCTTTGAAGGCTTTGCCTTGGGAGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGCCTTCTTCGCCATCACCACGCCCGTCGGGATCGCCATCGGCGCTGCGGTCTCCTCCACCTACAACCCCAACAGCACCGGGGCCTTGGTTGCTGAGGGCATTTTGGACTCTCTCTCGGCCGGAATTCTCGTCTATATGGCTCTGGTTGATTTGATCGCGGCCGACTTTCTTAGCAAGAGAATGAGCTGCAATTTCAGACTCCAAATTGTTTCGTATTGTACGCTATTTCTCGGCGCGGGATTGATGTCGTCGCTCGCGCTTTGGGCTTGATCTCAAATTCAATTACATTAGTCACTGCTTTGTTTATTTTATTTTATTTTTTTCTTGGGAGGAAATTGTTTCTTGAAGGTTGTTAGGAGGAAGGAAGCAAGAGGGTAGATGATGTTAGTGAGTATCCAACGCATTAGATAGATTCTGTAAACGTCAAATCTCTTCTGTAATTGCACAAAAATGCCCCAACTAAGT

mRNA sequence

ATGTTCTTCTTCGAGGATTTCTGGCAGATGCTCTGTATTCAAGGCTTCCGGGATAGAACTCGACTTCTCTCTGAGTCCCTTATACGGACCGTATCCGAATCCATGGGCAATTCGTCCTGTGGCGCCTCCGAGTTAGAACTCTGTCGAAACGACTCCGCTGCGCTTAATTTCAAATTAATCGCAATCGCCTCGATTCTCACATCGGGAGTCGTCGGAATCGTCATTCCGTTGTTCGGCAGACATCGCCGGTTCCTCAAGACCGACGGCAACCTGTTCGTGGCGGCGAAGGCTTTCGCCGCGGGGGTGATTCTGGCCACCGCGTTCGTCCACATGCTCCCCGACGGATCGAAAGCTCTGTCCGATCCGTGCTTGCCGGAATTCCCCTGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCTTCGCTGCTGACGCTCCTCGTCGATTTCGTCGGAACTCAGTACTACGAGCGGAAACAGGGGCTGATTCGATCGAAAGAGGAAGCTAGTCGGGTCGGGTCGGAGGTGGATTCCGGATTGGAATCCGGCATCCTCCCGGCCGCCGATTCGAACGCGAAAGTGTTCGGCGAAGAGGACGGCGGCGCGATGCACATCGTCGGAATCCATGCGCACGCGGCGCACCACCGGCACAGTCATCCGCAGGGGAAGGAGGCGTGTGACGGGCATGTGACACTCCATGACCATGGCCATGCCCATGGACATGGGCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCCTTAATTGCAGCCCTCTCCTTCCATCAGTTCTTTGAAGGCTTTGCCTTGGGAGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGCCTTCTTCGCCATCACCACGCCCGTCGGGATCGCCATCGGCGCTGCGGTCTCCTCCACCTACAACCCCAACAGCACCGGGGCCTTGGTTGCTGAGGGCATTTTGGACTCTCTCTCGGCCGGAATTCTCGTCTATATGGCTCTGGTTGATTTGATCGCGGCCGACTTTCTTAGCAAGAGAATGAGCTGCAATTTCAGACTCCAAATTGTTTCGTATTGTACGCTATTTCTCGGCGCGGGATTGATGTCGTCGCTCGCGCTTTGGGCTTGA

Coding sequence (CDS)

ATGTTCTTCTTCGAGGATTTCTGGCAGATGCTCTGTATTCAAGGCTTCCGGGATAGAACTCGACTTCTCTCTGAGTCCCTTATACGGACCGTATCCGAATCCATGGGCAATTCGTCCTGTGGCGCCTCCGAGTTAGAACTCTGTCGAAACGACTCCGCTGCGCTTAATTTCAAATTAATCGCAATCGCCTCGATTCTCACATCGGGAGTCGTCGGAATCGTCATTCCGTTGTTCGGCAGACATCGCCGGTTCCTCAAGACCGACGGCAACCTGTTCGTGGCGGCGAAGGCTTTCGCCGCGGGGGTGATTCTGGCCACCGCGTTCGTCCACATGCTCCCCGACGGATCGAAAGCTCTGTCCGATCCGTGCTTGCCGGAATTCCCCTGGTCGAAATTCCCCTTCTCTGGATTCTTCGCGATGATGGCTTCGCTGCTGACGCTCCTCGTCGATTTCGTCGGAACTCAGTACTACGAGCGGAAACAGGGGCTGATTCGATCGAAAGAGGAAGCTAGTCGGGTCGGGTCGGAGGTGGATTCCGGATTGGAATCCGGCATCCTCCCGGCCGCCGATTCGAACGCGAAAGTGTTCGGCGAAGAGGACGGCGGCGCGATGCACATCGTCGGAATCCATGCGCACGCGGCGCACCACCGGCACAGTCATCCGCAGGGGAAGGAGGCGTGTGACGGGCATGTGACACTCCATGACCATGGCCATGCCCATGGACATGGGCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCACATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGCACCATTAGACCCTTAATTGCAGCCCTCTCCTTCCATCAGTTCTTTGAAGGCTTTGCCTTGGGAGGCTGCATTTCTCAGGCTCAGTTCAACACCCTCTCCACCTCCCTCATGGCTGCCTTCTTCGCCATCACCACGCCCGTCGGGATCGCCATCGGCGCTGCGGTCTCCTCCACCTACAACCCCAACAGCACCGGGGCCTTGGTTGCTGAGGGCATTTTGGACTCTCTCTCGGCCGGAATTCTCGTCTATATGGCTCTGGTTGATTTGATCGCGGCCGACTTTCTTAGCAAGAGAATGAGCTGCAATTTCAGACTCCAAATTGTTTCGTATTGTACGCTATTTCTCGGCGCGGGATTGATGTCGTCGCTCGCGCTTTGGGCTTGA

Protein sequence

MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSGLESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Homology
BLAST of Spg025427 vs. NCBI nr
Match: XP_023546159.1 (zinc transporter 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 740.3 bits (1910), Expect = 8.8e-210
Identity = 380/416 (91.35%), Postives = 395/416 (94.95%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF ED WQMLC+QGF+D+ R+LS SLIRTVSESMGNS CG SELELCR+DSAA +FKLI
Sbjct: 1   MFFLEDLWQMLCVQGFQDKARVLSGSLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS VDSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEGNRVGSVVDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N KVFGEEDGGAMHIVG+HAHA HHRHSHPQGKEACDGHV +HDHGHAH
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIVGMHAHAGHHRHSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 -GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
            GHGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 241 GGHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIG AV+STYNPNSTGALVAEG
Sbjct: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALVAEG 360

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of Spg025427 vs. NCBI nr
Match: XP_004142927.1 (zinc transporter 4, chloroplastic isoform X1 [Cucumis sativus] >KGN62388.1 hypothetical protein Csa_018631 [Cucumis sativus])

HSP 1 Score: 738.4 bits (1905), Expect = 3.4e-209
Identity = 378/417 (90.65%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFFFED  QMLCI+GF+D+TRLLSES IRTVSESM NS+CG SELELCRNDSAAL+FKLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILT+GV+GI +PLFG+ RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEA+RVGS +D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHD--HGH 240
           LESGI+PA D N KVFGEE+GG MHIVG+HAHAAHHRH+HPQGKEACDGH+ +HD  HGH
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIA+GAAV+STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Spg025427 vs. NCBI nr
Match: KAG6598292.1 (Fe(2+) transport protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 738.4 bits (1905), Expect = 3.4e-209
Identity = 378/416 (90.87%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF E+ WQMLC+QGF+D+ R+LSESLIRTVS+SMGNS CG SELELCR+DSAA +FKLI
Sbjct: 200 MFFLENLWQMLCVQGFQDKARVLSESLIRTVSKSMGNSDCGVSELELCRDDSAAFHFKLI 259

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFV AKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 260 AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 319

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS  DSG
Sbjct: 320 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 379

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N KVFGEEDGGAMHI+G+HAHAAHHRHSHPQGKEACDGHV +HDHGHAH
Sbjct: 380 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHAH 439

Query: 241 -GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
            GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 440 GGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 499

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIG AV+STYNPNSTGAL+AEG
Sbjct: 500 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAEG 559

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 560 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 615

BLAST of Spg025427 vs. NCBI nr
Match: XP_022996460.1 (zinc transporter 4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 736.5 bits (1900), Expect = 1.3e-208
Identity = 377/416 (90.62%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF ED WQ+LC+QGF+D+ R+LSESL+RTVSESMGNS CG SELELCR+DSAA +FKLI
Sbjct: 1   MFFLEDLWQILCVQGFQDKARVLSESLLRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS VDSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVVDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N K+FGEEDGGAMHIVG+HAHA HHR SHPQGKEACDGHV +HDHGHAH
Sbjct: 181 LESGILPAGDLNVKLFGEEDGGAMHIVGMHAHAGHHRPSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 -GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
            GHGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 241 GGHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIGAAV+STYNPNSTGAL+AEG
Sbjct: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGAAVASTYNPNSTGALIAEG 360

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of Spg025427 vs. NCBI nr
Match: XP_022962014.1 (zinc transporter 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 735.7 bits (1898), Expect = 2.2e-208
Identity = 377/415 (90.84%), Postives = 394/415 (94.94%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF E+ WQMLC+QGF+D+ R+LSESLIRTVSESMGNS CG SELELCR+DSAA +FKLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFV AKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS  DSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N KVFGEEDGGAMHI+G+HAHAAHHRHSHPQGKEACDGHV +HDHGH  
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHG- 240

Query: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300
           GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH
Sbjct: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300

Query: 301 QFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGI 360
           QFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIG AV+STYNPNSTGAL+AEGI
Sbjct: 301 QFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAEGI 360

Query: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of Spg025427 vs. ExPASy Swiss-Prot
Match: Q8LE59 (Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT3 PE=2 SV=3)

HSP 1 Score: 525.0 bits (1351), Expect = 7.6e-148
Identity = 279/426 (65.49%), Postives = 330/426 (77.46%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLS--ESLIRTVSESMGNSS----CGASELELCRNDSAA 60
           MFF +  W+++ +  F   T+ LS  ES+++ V E+M  +S    C ASE +LCR+DSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV AKAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ    + E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQER-EASESVEPFG 180

Query: 181 SEVDSGLESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTL- 240
            E   G+   ++    ++ KVFGEED G +HIVGIHAHAAHHRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 ----HDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
               H HGH HGHGH H   G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+GI IG AV+S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 416
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of Spg025427 vs. ExPASy Swiss-Prot
Match: O04089 (Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 8.5e-139
Identity = 267/379 (70.45%), Postives = 303/379 (79.95%), Query Frame = 0

Query: 40  CGASELELCRNDSAALNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFA 99
           C A E +LCR+DSAA   K +AIASIL +G  G+ IPL GR+RRFL+T+GNLFVAAKAFA
Sbjct: 10  CDAGESDLCRDDSAAFLLKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFA 69

Query: 100 AGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYER 159
           AGVILAT FVHML  G++ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYER
Sbjct: 70  AGVILATGFVHMLAGGTEALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYER 129

Query: 160 KQGLIRSKEEASRVGSEVDSGLESGILPAAD---SNAKVFGEEDGGAMHIVGIHAHAAHH 219
           KQ   ++  EA+  GSE     E  ++P      ++ KVFGEEDGG +HIVGI AHAAHH
Sbjct: 130 KQERNQAATEAA-AGSE-----EIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAHH 189

Query: 220 RHSHPQGKEACDGHVTLHDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVI 279
           RHSH      CDG      H H H HGH HG    D E+G RHVVVSQILELGIVSHS+I
Sbjct: 190 RHSHSNSHGTCDG------HAHGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSII 249

Query: 280 IGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVG 339
           IGLSLGVS SPCTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+G
Sbjct: 250 IGLSLGVSQSPCTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLG 309

Query: 340 IAIGAAVSSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIV 399
           I IG AV+S++N +S GALV EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+V
Sbjct: 310 IGIGTAVASSFNSHSPGALVTEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVV 369

Query: 400 SYCTLFLGAGLMSSLALWA 416
           SY  LFLGAGLMS+LA+WA
Sbjct: 370 SYVMLFLGAGLMSALAIWA 374

BLAST of Spg025427 vs. ExPASy Swiss-Prot
Match: Q5Z653 (Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2)

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-116
Identity = 241/403 (59.80%), Postives = 279/403 (69.23%), Query Frame = 0

Query: 28  IRTVSESMGNSSC------GASELELCRNDSAALNFKLIAIASILTSGVVGIVIPLFGRH 87
           IR ++ S+  +SC      G  + E CR+++AAL  K++A+A+IL +G  G+ IPL GR 
Sbjct: 13  IRQIAASVSAASCDAVVGGGGDKDEECRDEAAALRLKMVAVAAILIAGAAGVAIPLVGRR 72

Query: 88  RR-------FLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPF 147
           RR          + G LFV AKAFAAGVILAT FVHML D   ALS+PCLP  PW +FPF
Sbjct: 73  RRGGGGGGGGGASSGGLFVLAKAFAAGVILATGFVHMLHDAEHALSNPCLPHSPWRRFPF 132

Query: 148 SGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSGL--ESGILPAADSN 207
            GF AM+A+L TL+VDFVGT +YERK      +EEA+    E  + L  + G LP  D  
Sbjct: 133 PGFVAMLAALATLVVDFVGTHFYERK----HRQEEAAAAAEEAAAALLEDGGALPVGDGE 192

Query: 208 AKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAHGHGHSHGLDGDD 267
            +        AMHIVGIHAHAA HRHSH     AC G      H H HGHGH  G     
Sbjct: 193 GRDGRGGKRDAMHIVGIHAHAAAHRHSHAHVHGACHGGAVNDAHAHGHGHGHEEG----- 252

Query: 268 DESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCI 327
                RHVVVSQILELGIVSHSVIIGLSLGVS SPCTI+PL+AALSFHQFFEGFALGGCI
Sbjct: 253 --PSARHVVVSQILELGIVSHSVIIGLSLGVSQSPCTIKPLVAALSFHQFFEGFALGGCI 312

Query: 328 SQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGILDSLSAGILVYM 387
           S+AQ    S  LMA FFAITTP GI +GAAV+S YNPNS  ALV EGILDS+SAGIL+YM
Sbjct: 313 SEAQLKNFSAFLMAFFFAITTPAGITVGAAVASFYNPNSPRALVVEGILDSMSAGILIYM 372

Query: 388 ALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           ALVDLIAADFLS++MSCN RLQ+ SY  LFLGA  M++LALWA
Sbjct: 373 ALVDLIAADFLSRKMSCNPRLQVGSYIALFLGAMAMAALALWA 404

BLAST of Spg025427 vs. ExPASy Swiss-Prot
Match: O82643 (Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 6.1e-113
Identity = 231/363 (63.64%), Postives = 274/363 (75.48%), Query Frame = 0

Query: 62  IASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G+ IPL G     L  +G L   AKAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEV---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ  + ++   S +G++V    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKMMNQSVES-LGTQVSVMS 120

Query: 182 DSGLESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACD-----GHVT 241
           D GLESG L           +EDGGA+HIVG+ AHA HHRHS   G E  +       V+
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 LHDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRP 301
            H HGH+HGHG   GLD     SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRP
Sbjct: 181 GHGHGHSHGHG-DVGLD-----SGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRP 240

Query: 302 LIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNST 361
           L+ ALSFHQFFEGFALGGC+++A+     +++MA FFAITTP+G+A+G A++S+YN  S 
Sbjct: 241 LLLALSFHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSV 300

Query: 362 GALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLA 416
            ALVAEG+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS+LA
Sbjct: 301 AALVAEGVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALA 344

BLAST of Spg025427 vs. ExPASy Swiss-Prot
Match: Q6L8F7 (Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-98
Identity = 212/370 (57.30%), Postives = 255/370 (68.92%), Query Frame = 0

Query: 48  CRNDSAALNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATA 107
           CR+D+AAL  K +A+A+IL +GVVG+ +PL GR RR L+TD   FVAAKAFAAGVILAT 
Sbjct: 36  CRDDAAALRLKGVAMATILVAGVVGVGLPLAGRKRRALRTDSAAFVAAKAFAAGVILATG 95

Query: 108 FVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSK 167
           FVHML D   ALS PCLP  PW  FPF GF AM A+L TL++DF+ T++YE K      +
Sbjct: 96  FVHMLHDAEHALSSPCLPAHPWRSFPFPGFVAMSAALATLVLDFLATRFYEGKH-----R 155

Query: 168 EEASRVGSEVDSGLESGILPAADSNAKVFGEEDG-GAMHIVGIHAHAAHHRHSHPQGKEA 227
            E  RV +   + L +      D       E+D      ++  H+H+    HSHP     
Sbjct: 156 AETERVKAAAAAALAASSASDDDITVVTVTEDDNDNKAPLLQPHSHS----HSHP----- 215

Query: 228 CDGHVTLHDHGHAHGHGHSHGLDGDDD-ESGVRHVVVSQILELGIVSHSVIIGLSLGVSH 287
                  H HGH H      G  G+ +  + VR VVVSQILE+GIVSHSVIIGLSLGVS 
Sbjct: 216 -------HGHGHGHELAQPEGSGGEGEVPAQVRSVVVSQILEMGIVSHSVIIGLSLGVSR 275

Query: 288 SPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSS 347
           SPCTIRPL+AALSFHQFFEGFALGGCI+QAQF TLS ++MA FFAITTP GIA GA V+S
Sbjct: 276 SPCTIRPLVAALSFHQFFEGFALGGCIAQAQFKTLSAAIMACFFAITTPAGIAAGAGVAS 335

Query: 348 TYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGA 407
            YN NS  ALV EGILDS+SAGIL+YM+LVDLIAADFL  +M+ + R Q+++Y  LFLGA
Sbjct: 336 FYNANSPRALVVEGILDSVSAGILIYMSLVDLIAADFLGGKMTGSTRQQVMAYIALFLGA 384

Query: 408 GLMSSLALWA 416
             MSSLA+WA
Sbjct: 396 LSMSSLAIWA 384

BLAST of Spg025427 vs. ExPASy TrEMBL
Match: A0A0A0LKS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.6e-209
Identity = 378/417 (90.65%), Postives = 401/417 (96.16%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFFFED  QMLCI+GF+D+TRLLSES IRTVSESM NS+CG SELELCRNDSAAL+FKLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILT+GV+GI +PLFG+ RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEA+RVGS +D+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHD--HGH 240
           LESGI+PA D N KVFGEE+GG MHIVG+HAHAAHHRH+HPQGKEACDGH+ +HD  HGH
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIA+GAAV+STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Spg025427 vs. ExPASy TrEMBL
Match: A0A6J1K6U3 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491704 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.2e-209
Identity = 377/416 (90.62%), Postives = 396/416 (95.19%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF ED WQ+LC+QGF+D+ R+LSESL+RTVSESMGNS CG SELELCR+DSAA +FKLI
Sbjct: 1   MFFLEDLWQILCVQGFQDKARVLSESLLRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS VDSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVVDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N K+FGEEDGGAMHIVG+HAHA HHR SHPQGKEACDGHV +HDHGHAH
Sbjct: 181 LESGILPAGDLNVKLFGEEDGGAMHIVGMHAHAGHHRPSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 -GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
            GHGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 241 GGHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIGAAV+STYNPNSTGAL+AEG
Sbjct: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGAAVASTYNPNSTGALIAEG 360

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of Spg025427 vs. ExPASy TrEMBL
Match: A0A6J1HFN1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462610 PE=3 SV=1)

HSP 1 Score: 735.7 bits (1898), Expect = 1.1e-208
Identity = 377/415 (90.84%), Postives = 394/415 (94.94%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFF E+ WQMLC+QGF+D+ R+LSESLIRTVSESMGNS CG SELELCR+DSAA +FKLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFG  RRFLKTDGNLFV AKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEE +RVGS  DSG
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LESGILPA D N KVFGEEDGGAMHI+G+HAHAAHHRHSHPQGKEACDGHV +HDHGH  
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHG- 240

Query: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300
           GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH
Sbjct: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300

Query: 301 QFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGI 360
           QFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIAIG AV+STYNPNSTGAL+AEGI
Sbjct: 301 QFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAEGI 360

Query: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA
Sbjct: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of Spg025427 vs. ExPASy TrEMBL
Match: A0A1S3BAD0 (zinc transporter 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487782 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.4e-208
Identity = 378/417 (90.65%), Postives = 400/417 (95.92%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           MFFFED  Q+LCI+GF+D+TRLLSES IRTVSESM NS+CG SE+ELCRNDSAAL+FKLI
Sbjct: 1   MFFFEDLLQVLCIRGFQDKTRLLSESFIRTVSESMTNSACGVSEVELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILT+GV+GI IPLFG+ RRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTAGVIGIAIPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG+IRSKEEA+RVGS VD+G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGMIRSKEEANRVGSLVDTG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHD--HGH 240
           LESGI+PA + N KVFGEEDGG MHIVG+HAHAAHHRH+HPQGKEACDGHV +HD  HGH
Sbjct: 181 LESGIVPAGELNVKVFGEEDGGGMHIVGMHAHAAHHRHNHPQGKEACDGHVAVHDHGHGH 240

Query: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
           AHGHGHSHGLD DDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDVDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMA FFAITTP+GIA+GAAV+STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCTLFLGAGLMSSLALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of Spg025427 vs. ExPASy TrEMBL
Match: A0A6J1GGQ1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111454070 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.0e-208
Identity = 380/415 (91.57%), Postives = 393/415 (94.70%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSSCGASELELCRNDSAALNFKLI 60
           M FFED WQML  +GF+D+TRLLSES++RTVSESMGNSSC  SELELCR+DSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV+GI IPLFGRHRRFLKTDGNLFVA KAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEVDSG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE +RVGS +DSG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTLHDHGHAH 240
           LE+GILPAADSN KVFGEEDGG MHIVG+HAHAA HRHSHPQGK+ACDGHVTLHD    H
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHD----H 240

Query: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300
           GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH
Sbjct: 241 GHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFH 300

Query: 301 QFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGI 360
           QFFEGFALGGCISQAQFNTLSTSLMA FFA TTPVGIAIGAA+SSTYNPNSTGALVAEGI
Sbjct: 301 QFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGI 360

Query: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 LDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of Spg025427 vs. TAIR 10
Match: AT1G60960.1 (iron regulated transporter 3 )

HSP 1 Score: 525.0 bits (1351), Expect = 5.4e-149
Identity = 279/426 (65.49%), Postives = 330/426 (77.46%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLS--ESLIRTVSESMGNSS----CGASELELCRNDSAA 60
           MFF +  W+++ +  F   T+ LS  ES+++ V E+M  +S    C ASE +LCR+DSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV AKAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ    + E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQER-EASESVEPFG 180

Query: 181 SEVDSGLESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTL- 240
            E   G+   ++    ++ KVFGEED G +HIVGIHAHAAHHRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 ----HDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
               H HGH HGHGH H   G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+GI IG AV+S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 416
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of Spg025427 vs. TAIR 10
Match: AT1G10970.1 (zinc transporter 4 precursor )

HSP 1 Score: 509.2 bits (1310), Expect = 3.1e-144
Identity = 278/422 (65.88%), Postives = 325/422 (77.01%), Query Frame = 0

Query: 1   MFFFEDFWQMLCIQGFRDRTRLLSESLIRTVSESMGNSS----CGASELELCRNDSAALN 60
           M F +  W++  +  F      LSES+++ + E+M +S+    C A E +LCR+DSAA  
Sbjct: 1   MIFVDVLWKLFPLYSFGSGRDSLSESILQIIPETMASSTTKILCDAGESDLCRDDSAAFL 60

Query: 61  FKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGS 120
            K +AIASIL +G  G+ IPL GR+RRFL+T+GNLFVAAKAFAAGVILAT FVHML  G+
Sbjct: 61  LKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGT 120

Query: 121 KALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSE 180
           +ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYERKQ   ++  EA+  GSE
Sbjct: 121 EALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYERKQERNQAATEAA-AGSE 180

Query: 181 VDSGLESGILPAAD---SNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACDGHVTL 240
                E  ++P      ++ KVFGEEDGG +HIVGI AHAAHHRHSH      CDG    
Sbjct: 181 -----EIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAHHRHSHSNSHGTCDG---- 240

Query: 241 HDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPL 300
             H H H HGH HG    D E+G RHVVVSQILELGIVSHS+IIGLSLGVS SPCTIRPL
Sbjct: 241 --HAHGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSIIIGLSLGVSQSPCTIRPL 300

Query: 301 IAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNSTG 360
           IAALSFHQFFEGFALGGCISQAQF   S ++MA FFA+TTP+GI IG AV+S++N +S G
Sbjct: 301 IAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGIGTAVASSFNSHSPG 360

Query: 361 ALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLAL 416
           ALV EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+VSY  LFLGAGLMS+LA+
Sbjct: 361 ALVTEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYVMLFLGAGLMSALAI 408

BLAST of Spg025427 vs. TAIR 10
Match: AT4G33020.1 (ZIP metal ion transporter family )

HSP 1 Score: 409.1 bits (1050), Expect = 4.3e-114
Identity = 231/363 (63.64%), Postives = 274/363 (75.48%), Query Frame = 0

Query: 62  IASILTSGVVGIVIPLFGRHRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G+ IPL G     L  +G L   AKAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGLIRSKEEASRVGSEV---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ  + ++   S +G++V    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKMMNQSVES-LGTQVSVMS 120

Query: 182 DSGLESGILPAADSNAKVFGEEDGGAMHIVGIHAHAAHHRHSHPQGKEACD-----GHVT 241
           D GLESG L           +EDGGA+HIVG+ AHA HHRHS   G E  +       V+
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 LHDHGHAHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRP 301
            H HGH+HGHG   GLD     SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRP
Sbjct: 181 GHGHGHSHGHG-DVGLD-----SGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRP 240

Query: 302 LIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAAFFAITTPVGIAIGAAVSSTYNPNST 361
           L+ ALSFHQFFEGFALGGC+++A+     +++MA FFAITTP+G+A+G A++S+YN  S 
Sbjct: 241 LLLALSFHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSV 300

Query: 362 GALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLA 416
            ALVAEG+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS+LA
Sbjct: 301 AALVAEGVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALA 344

BLAST of Spg025427 vs. TAIR 10
Match: AT1G05300.1 (zinc transporter 5 precursor )

HSP 1 Score: 275.0 bits (702), Expect = 9.8e-74
Identity = 164/389 (42.16%), Postives = 233/389 (59.90%), Query Frame = 0

Query: 31  VSESMGNSSCGASELELCRNDSAALNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGN 90
           ++ S G S C  S  +   N + A  +K+ AI S+L +GV+G++ PL G+    LK +  
Sbjct: 22  IAVSAGESKCECSHEDDEANKAGAKKYKIAAIPSVLAAGVIGVMFPLLGKFFPSLKPETT 81

Query: 91  LFVAAKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVD 150
            F   KAFAAGVILAT F+H+LP+G + L+ PCL    W +FPF+GF AM+A++LTL VD
Sbjct: 82  FFFVTKAFAAGVILATGFMHVLPEGYEKLTSPCLKGEAW-EFPFTGFIAMVAAILTLSVD 141

Query: 151 FVGTQYYERKQGLIRSKEEASRVGSEVDSGLESGILPAADSNAKVFGEEDGGAMHIVGIH 210
              T Y+ +        + + R+G   +     G                GG    +G+H
Sbjct: 142 SFATSYFHKAH-----FKTSKRIGDGEEQDAGGG----------------GGGGDELGLH 201

Query: 211 AHAAHHRHSHPQGKEACDGHVTLHDHGHAHGHGHSHGLDG-DDDESGV---RHVVVSQIL 270
            HA                            HGH+HG+ G +  ES V   R  VV+Q+L
Sbjct: 202 VHA----------------------------HGHTHGIVGVESGESQVQLHRTRVVAQVL 261

Query: 271 ELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMA 330
           E+GI+ HSV+IG+SLG S SP T + L AAL FHQ FEG  LGGCI+Q  FN +S ++M+
Sbjct: 262 EVGIIVHSVVIGISLGASQSPDTAKALFAALMFHQCFEGLGLGGCIAQGNFNCMSITIMS 321

Query: 331 AFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKR 390
            FF++TTPVGIA+G A+SS+Y+ +S  AL+ +G+L++ SAGIL+YM+LVD +AADF+  +
Sbjct: 322 IFFSVTTPVGIAVGMAISSSYDDSSPTALIVQGVLNAASAGILIYMSLVDFLAADFMHPK 360

Query: 391 MSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           M  N RLQI+++ +L +GAG+MS LA WA
Sbjct: 382 MQSNTRLQIMAHISLLVGAGVMSLLAKWA 360

BLAST of Spg025427 vs. TAIR 10
Match: AT5G62160.1 (zinc transporter 12 precursor )

HSP 1 Score: 258.1 bits (658), Expect = 1.2e-68
Identity = 168/392 (42.86%), Postives = 226/392 (57.65%), Query Frame = 0

Query: 31  VSESMGNSSCGASELELCRNDSAALNFKLIAIASILTSGVVGIVIPLFGRHRRFLKTDGN 90
           VS +   + CG S+       ++AL +K+IA  SIL +GV G+ +P+FG     LKT+ N
Sbjct: 23  VSAAEEENQCGGSKGGSAAEKASALKYKIIAFFSILIAGVFGVCLPIFG-----LKTESN 82

Query: 91  LFVAAKAFAAGVILATAFVHMLPDGSKALSDPCL-PEFPWSKFPFSGFFAMMASLLTLLV 150
            F+  KAFAAGVILAT FVH+LPD +++L+  CL  E PW  FP +G  AM AS+LT+L+
Sbjct: 83  FFMYVKAFAAGVILATGFVHILPDATESLTSSCLGEEPPWGDFPMTGLVAMAASILTMLI 142

Query: 151 DFVGTQYYERKQGLIRSKEEASRVGSEVDSGLESGILPAADSNAKVFGEEDGGAMHIVGI 210
           +   + Y  R           SR+        E   LP +       GEE+         
Sbjct: 143 ESFASGYLNR-----------SRLAK------EGKTLPVSTG-----GEEE--------- 202

Query: 211 HAHAAHHRHSHPQGKEACDGHVTLHDHGHAHGH---GHSHG---LDGDDDESGVRHVVVS 270
           HAH                        G AH H   GHSHG   +  DDD   +R  +V+
Sbjct: 203 HAHT-----------------------GSAHTHASQGHSHGSLLIPQDDDHIDMRKKIVT 262

Query: 271 QILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTS 330
           QILELGIV HSVIIG+SLG S S  TI+PLIAA++FHQ FEGF LGGCIS+A+F      
Sbjct: 263 QILELGIVVHSVIIGISLGASPSVSTIKPLIAAITFHQLFEGFGLGGCISEAKFRVKKIW 322

Query: 331 LMAAFFAITTPVGIAIGAAVSSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFL 390
           +M  FFA+T P+GI IG  V+  YN NS  AL   G L++ ++GIL+YMALVDL+A  F+
Sbjct: 323 VMLMFFALTAPIGIGIGIGVAEIYNENSPMALKVSGFLNATASGILIYMALVDLVAPLFM 355

Query: 391 SKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416
           +++   + ++Q+    +L +GAGLMS LA+WA
Sbjct: 383 NQKTQSSMKIQVACSVSLVVGAGLMSLLAIWA 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546159.18.8e-21091.35zinc transporter 4, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_004142927.13.4e-20990.65zinc transporter 4, chloroplastic isoform X1 [Cucumis sativus] >KGN62388.1 hypot... [more]
KAG6598292.13.4e-20990.87Fe(2+) transport protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_022996460.11.3e-20890.63zinc transporter 4, chloroplastic-like [Cucurbita maxima][more]
XP_022962014.12.2e-20890.84zinc transporter 4, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8LE597.6e-14865.49Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT... [more]
O040898.5e-13970.45Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 S... [more]
Q5Z6531.2e-11659.80Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2[more]
O826436.1e-11363.64Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1[more]
Q6L8F71.1e-9857.30Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LKS61.6e-20990.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1[more]
A0A6J1K6U36.2e-20990.63zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114917... [more]
A0A6J1HFN11.1e-20890.84zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A1S3BAD01.4e-20890.65zinc transporter 4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487782 PE=3 S... [more]
A0A6J1GGQ14.0e-20891.57zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
Match NameE-valueIdentityDescription
AT1G60960.15.4e-14965.49iron regulated transporter 3 [more]
AT1G10970.13.1e-14465.88zinc transporter 4 precursor [more]
AT4G33020.14.3e-11463.64ZIP metal ion transporter family [more]
AT1G05300.19.8e-7442.16zinc transporter 5 precursor [more]
AT5G62160.11.2e-6842.86zinc transporter 12 precursor [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003689Zinc/iron permeasePFAMPF02535Zipcoord: 56..412
e-value: 2.3E-76
score: 257.2
IPR004698Zinc/iron permease, fungal/plantTIGRFAMTIGR00820TIGR00820coord: 40..415
e-value: 5.1E-132
score: 437.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..253
NoneNo IPR availablePANTHERPTHR11040ZINC/IRON TRANSPORTERcoord: 35..415
NoneNo IPR availablePANTHERPTHR11040:SF157ZIP ZINC/IRON TRANSPORT FAMILY PROTEINcoord: 35..415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025427.1Spg025427.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity