Spg025384 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg025384 vs. NCBI nr
Match: KGN62538.1 (hypothetical protein Csa_021888 [Cucumis sativus])

HSP 1 Score: 597.4 bits (1539), Expect = 7.3e-167
Identity = 279/324 (86.11%), Postives = 295/324 (91.05%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+G DFVP  TDPLTGVTSKDVT+ P FGVSAR 
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKA EHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPE+W+NKH DF RVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDHMFHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIRRLG FFN DM P L
Sbjct: 301 EGQKAKDLIRRLGDFFNRDMPPSL 324

BLAST of Spg025384 vs. NCBI nr
Match: XP_011649590.2 (probable carboxylesterase 2 [Cucumis sativus])

HSP 1 Score: 597.4 bits (1539), Expect = 7.3e-167
Identity = 279/324 (86.11%), Postives = 295/324 (91.05%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+G DFVP  TDPLTGVTSKDVT+ P FGVSAR 
Sbjct: 19  MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 78

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKA EHP
Sbjct: 79  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 138

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPE+W+NKH DF RVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 139 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 198

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 199 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 258

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDHMFHLNDL
Sbjct: 259 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 318

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIRRLG FFN DM P L
Sbjct: 319 EGQKAKDLIRRLGDFFNRDMPPSL 342

BLAST of Spg025384 vs. NCBI nr
Match: KAA0060874.1 (putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 593.6 bits (1529), Expect = 1.0e-165
Identity = 278/324 (85.80%), Postives = 293/324 (90.43%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+ TDFVPP TDPLTGVTSKDVT  P FGVSAR 
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKA EHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPEIWLNKH DF RVFLAGASAGANIAHNLAMV GDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 181 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIR LG FFN DM P L
Sbjct: 301 EGQKAKDLIRDLGDFFNRDMPPSL 324

BLAST of Spg025384 vs. NCBI nr
Match: XP_008444640.2 (PREDICTED: probable carboxylesterase 2 [Cucumis melo])

HSP 1 Score: 591.7 bits (1524), Expect = 4.0e-165
Identity = 277/324 (85.49%), Postives = 292/324 (90.12%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+ TDFVPP TDPLTGVTSKDVT  P FGVSAR 
Sbjct: 32  MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 91

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKA EHP
Sbjct: 92  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 151

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPEIWLNKH DF RVFLAGASAGANIAHNLAMV GDPD
Sbjct: 152 IPTAYEDSWAALQWVISHLDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 211

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 212 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 271

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDH+FHLND 
Sbjct: 272 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDS 331

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIR LG FFN DM P L
Sbjct: 332 EGQKAKDLIRDLGDFFNRDMPPSL 355

BLAST of Spg025384 vs. NCBI nr
Match: XP_038885275.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-164
Identity = 277/326 (84.97%), Postives = 295/326 (90.49%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESG+ E  FELLPLLRV+KNGRIERL+GTDFVPP TDPLTGVTSKDV I PEFG+SAR 
Sbjct: 1   MESGKPEVAFELLPLLRVYKNGRIERLVGTDFVPPGTDPLTGVTSKDVMILPEFGISARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLP LT  TQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEA+VVAVSVNYRKA EHP
Sbjct: 61  FLPILTRSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEARVVAVSVNYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IPAAYEDSWAALQW ISH D KGPEIWLN+H DF RVFL GASAGANIAHNLAMVAGDPD
Sbjct: 121 IPAAYEDSWAALQWVISHRDEKGPEIWLNEHVDFRRVFLVGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVN+ L+GVALEHPYFWGSVRIGKEAG+PV+A LFD+LWGF+CP RP+NDDPWVN VAE
Sbjct: 181 CGVNLELIGVALEHPYFWGSVRIGKEAGNPVKARLFDQLWGFICPVRPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GAARLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW+GVAEI ETEDEDH+FHLNDL
Sbjct: 241 GAARLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGSSGWLGVAEIEETEDEDHLFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPLPF 327
           E QKAKDLI+RLG FFN DM P L F
Sbjct: 301 EGQKAKDLIQRLGDFFNRDMPPSLLF 326

BLAST of Spg025384 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.2e-81
Identity = 147/317 (46.37%), Postives = 201/317 (63.41%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MES + +   ELLP L VH +G +ERL GT+  PP  DP+TGV SKD+ I P+ G+SAR 
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           + P    P Q++PL++YFHGG F   S     YH  LN +  +A V+AVSVNYR A EHP
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           +P AYEDSW AL+  I   +    E W+N +AD   +FL G SAGANI+H+LA  A   D
Sbjct: 121 LPTAYEDSWTALK-NIQAIN----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
               + + G+ + HPYFWG+  IG E  D  R  + D  W FVCP+   +DDPW+N  A+
Sbjct: 181 --QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           G+  L G+G  RV++ VAEKD+L +RG +YY  L  S W G  EI+ET+++DH+FH+ + 
Sbjct: 241 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 300

Query: 301 ESQKAKDLIRRLGAFFN 318
           +  +A +++R L  F N
Sbjct: 301 DCDEAMEMVRCLALFIN 310

BLAST of Spg025384 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.7e-79
Identity = 149/327 (45.57%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLPN 64
           ++E   +  P+L ++K+GRIERL+G   VPPS++P  GV SKDV   P+  +S R +LP 
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPE 61

Query: 65  L-----THPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEH 124
                 T  + +LPL+VYFHGG F  ++ F+  YH +L A  + +  VAVSV+YR+A EH
Sbjct: 62  KAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEH 121

Query: 125 PIPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAG-- 184
           PIP +Y+DSW AL+W  SH  G G E WLNKHADFS+VFLAG SAGANI H++ M A   
Sbjct: 122 PIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKD 181

Query: 185 --DPDCGVNIGLLGVALEHPYFWGSVRI-GKEAGDPVRAGLFDRLWGFVCPARPD-NDDP 244
              P+     G+ G+ L HPYFW    +  KE  D       + +W    P   D +DDP
Sbjct: 182 KLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDP 241

Query: 245 WVNSVAEGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIG-VAEIVETEDED 304
           ++N V   +  L+G+G G+VLV VAEKD L  +GW Y+  LG S W G V ++VET+ E 
Sbjct: 242 FINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEG 301

Query: 305 HMFHLNDLESQKAKDLIRRLGAFFNSD 320
           H+FHL D  S+KA +L+ R   F   D
Sbjct: 302 HVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of Spg025384 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.1e-76
Identity = 141/314 (44.90%), Postives = 194/314 (61.78%), Query Frame = 0

Query: 7   ETVFELLPLLRVHKNGRIERLIGTDFVPPS-TDPLTGVTSKDVTIFPEFGVSARPFLPNL 66
           E V ELLPL+RV+K+G +ERL+ ++ V  S  DP TGV+SKD+ I     VSAR FLP  
Sbjct: 4   EIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKS 63

Query: 67  THPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPIPAAY 126
            H   +LP+ +YFHGG FC +S F+   H YLN L +EA ++A+SV++R    HPIPAAY
Sbjct: 64  HHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAY 123

Query: 127 EDSWAALQWTISHYDG---KGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPDCG 186
           ED W  L+W  SH +      PE WL  HADF++V++ G ++GANIAHNL + AG+    
Sbjct: 124 EDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLP 183

Query: 187 VNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDN-DDPWVNSVAEG 246
            ++ +LG  L  P+FWGS  IG EA +     L  ++W F CP  P   D+PW+N    G
Sbjct: 184 GDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPG 243

Query: 247 AARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDLE 306
           A  LA +   ++LV +  KD  RDR  LY+H +  SGW G  ++ +  DE+H F L   E
Sbjct: 244 APSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPE 303

Query: 307 SQKAKDLIRRLGAF 316
           +  AK +I+RL +F
Sbjct: 304 THLAKAMIKRLASF 317

BLAST of Spg025384 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 4.9e-73
Identity = 142/318 (44.65%), Postives = 190/318 (59.75%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLPN 64
           ++E   +  PLL+++K+GRIERL+G   VPPS++P  GV SKDV    +  +S R +LP 
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPE 61

Query: 65  --LTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPIP 124
                   +LPL+VYFHGG F  ++ F+  YH +L    + +  VAVSV+YR+A EHPI 
Sbjct: 62  KAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 121

Query: 125 AAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPDC- 184
             ++DSW AL+W  +H  G G E WLNKHADFSRVFL+G SAGANI H++AM A      
Sbjct: 122 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 181

Query: 185 -GVN-IGLLGVALEHPYFWGSVRIG-KEAGDPVRAGLFDRLWGFVCPARPD-NDDPWVNS 244
            G+N  G+ G+ L HPYFW    I  K+  D       +  W    P   D  DDP +N 
Sbjct: 182 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 241

Query: 245 VAEGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHL 304
           V   +  L+G+G G+VLV VAEKD L  +GW Y   L  SGW G  E+VE+E EDH+FHL
Sbjct: 242 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 301

Query: 305 NDLESQKAKDLIRRLGAF 316
              E   A +++ +   F
Sbjct: 302 LKPECDNAIEVMHKFSGF 319

BLAST of Spg025384 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 142/320 (44.38%), Postives = 194/320 (60.62%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLP- 64
           ++E  F+  P  R+ KNG IERL+   FVPPS +P  GV SKD    PE  +S R +LP 
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQ 61

Query: 65  NLTHPT--QRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPI 124
           N  + T  +++PL+VYFHGG F  ++ F+  YH +L +  +    +AVSV YR+A EHPI
Sbjct: 62  NSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPI 121

Query: 125 PAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAM-VAGDPD 184
           P  YEDSW A+QW  +H    GPE WLNKHADFS+VFLAG SAGANIAH++A+ V  +  
Sbjct: 122 PTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKL 181

Query: 185 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDN-DDPWVNSVA 244
              N  + G+ L HPYF     I +   + +R   ++RLW    P   +  +DPW+N V 
Sbjct: 182 PPENFKISGMILFHPYFLSKALIEEMEVEAMR--YYERLWRIASPDSGNGVEDPWINVV- 241

Query: 245 EGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLND 304
              + L G+G  RVLV VA  DVL   GW Y   L  SGWIG  +++ET++E H+FHL D
Sbjct: 242 --GSDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 301

Query: 305 LESQKAKDLIRRLGAFFNSD 320
            +S+ A+ ++R    F   +
Sbjct: 302 PDSENARRVLRNFAEFLKEE 316

BLAST of Spg025384 vs. ExPASy TrEMBL
Match: A0A0A0LLA8 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360060 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.5e-167
Identity = 279/324 (86.11%), Postives = 295/324 (91.05%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+G DFVP  TDPLTGVTSKDVT+ P FGVSAR 
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKA EHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPE+W+NKH DF RVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDHMFHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIRRLG FFN DM P L
Sbjct: 301 EGQKAKDLIRRLGDFFNRDMPPSL 324

BLAST of Spg025384 vs. ExPASy TrEMBL
Match: A0A5A7V0M5 (Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00060 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.1e-166
Identity = 278/324 (85.80%), Postives = 293/324 (90.43%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+ TDFVPP TDPLTGVTSKDVT  P FGVSAR 
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKA EHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPEIWLNKH DF RVFLAGASAGANIAHNLAMV GDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 181 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIR LG FFN DM P L
Sbjct: 301 EGQKAKDLIRDLGDFFNRDMPPSL 324

BLAST of Spg025384 vs. ExPASy TrEMBL
Match: A0A1S3BBJ6 (probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103487910 PE=4 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 1.9e-165
Identity = 277/324 (85.49%), Postives = 292/324 (90.12%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPL+R++KNGRIERL+ TDFVPP TDPLTGVTSKDVT  P FGVSAR 
Sbjct: 32  MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 91

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKA EHP
Sbjct: 92  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 151

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWAALQW ISH DGKGPEIWLNKH DF RVFLAGASAGANIAHNLAMV GDPD
Sbjct: 152 IPTAYEDSWAALQWVISHLDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 211

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
           CGVNI L+GVALEHPYFWGSVRIGKEA +PV+A LFD+LWGF+CPARP+NDDPWVN VAE
Sbjct: 212 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 271

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRG LY+ ALG SGW GVAEIVETEDEDH+FHLND 
Sbjct: 272 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDS 331

Query: 301 ESQKAKDLIRRLGAFFNSDMAPPL 325
           E QKAKDLIR LG FFN DM P L
Sbjct: 332 EGQKAKDLIRDLGDFFNRDMPPSL 355

BLAST of Spg025384 vs. ExPASy TrEMBL
Match: A0A6J1K6U8 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111491707 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.8e-163
Identity = 275/320 (85.94%), Postives = 292/320 (91.25%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MESGE ET FELLPLLRV+KNGRIERLIGTDFVPP TDPLTGVTSKDVTIFPE GVSAR 
Sbjct: 1   MESGEPETAFELLPLLRVYKNGRIERLIGTDFVPPGTDPLTGVTSKDVTIFPESGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEA+V+AVSV+YRKA EHP
Sbjct: 61  FLPNLTHSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEARVLAVSVDYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           +P AYEDSWAALQW ISH +GKGPE+WLNKHADF R+FLAGASAGANIAHNLAM+ G+PD
Sbjct: 121 VPTAYEDSWAALQWAISHCEGKGPEVWLNKHADFRRLFLAGASAGANIAHNLAMLDGEPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
            G N+ L+GVALEHPYFWGSVR  KEA DP+R  LFDRLWGF+CPARP+NDDPWVN VAE
Sbjct: 181 YGENMELVGVALEHPYFWGSVRTAKEAEDPLRTTLFDRLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GAARLAGMGSGRVLVCVAEKDVLRDRG  YY AL  SGW+GVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGRHYYEALAGSGWLGVAEIVETEDEDHLFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDM 321
           E QKAKDLIRRLGAFFN DM
Sbjct: 301 EGQKAKDLIRRLGAFFNRDM 320

BLAST of Spg025384 vs. ExPASy TrEMBL
Match: A0A6J1HDJ9 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111462621 PE=4 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.4e-162
Identity = 274/320 (85.62%), Postives = 290/320 (90.62%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           ME GE ET FELLPLLRV+KNGRIERLIGTDFVPP TDPLT VTSKDVTIFPE GVSAR 
Sbjct: 1   MEFGEPETAFELLPLLRVYKNGRIERLIGTDFVPPGTDPLTSVTSKDVTIFPESGVSARL 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           FLPNLTH TQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKV+AVSVNYRKA EHP
Sbjct: 61  FLPNLTHSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVLAVSVNYRKAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           IPAAYEDSWAALQW ISH +GKGPE+WLNKHADF R+FLAGASAGANIAHNLAM+AG+PD
Sbjct: 121 IPAAYEDSWAALQWAISHCEGKGPEVWLNKHADFRRLFLAGASAGANIAHNLAMLAGEPD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
            G N+ L+GVALEHPYFWGS R  KEA D +R  LF+RLWGF+CPARP+NDDPWVN VAE
Sbjct: 181 SGENMELVGVALEHPYFWGSARTAKEAEDSMRTRLFERLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           GAARLAGMGSGRVLVCVAEKDVLRDRG  YY AL  SGW+GVAEIVE+EDEDH+FHLNDL
Sbjct: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGRHYYEALAGSGWLGVAEIVESEDEDHLFHLNDL 300

Query: 301 ESQKAKDLIRRLGAFFNSDM 321
           E QKAKDLIRRLGAFFN DM
Sbjct: 301 EGQKAKDLIRRLGAFFNRDM 320

BLAST of Spg025384 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.5e-82
Identity = 147/317 (46.37%), Postives = 201/317 (63.41%), Query Frame = 0

Query: 1   MESGEAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARP 60
           MES + +   ELLP L VH +G +ERL GT+  PP  DP+TGV SKD+ I P+ G+SAR 
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  FLPNLTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHP 120
           + P    P Q++PL++YFHGG F   S     YH  LN +  +A V+AVSVNYR A EHP
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 IPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPD 180
           +P AYEDSW AL+  I   +    E W+N +AD   +FL G SAGANI+H+LA  A   D
Sbjct: 121 LPTAYEDSWTALK-NIQAIN----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDNDDPWVNSVAE 240
               + + G+ + HPYFWG+  IG E  D  R  + D  W FVCP+   +DDPW+N  A+
Sbjct: 181 --QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 240

Query: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDL 300
           G+  L G+G  RV++ VAEKD+L +RG +YY  L  S W G  EI+ET+++DH+FH+ + 
Sbjct: 241 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 300

Query: 301 ESQKAKDLIRRLGAFFN 318
           +  +A +++R L  F N
Sbjct: 301 DCDEAMEMVRCLALFIN 310

BLAST of Spg025384 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 297.0 bits (759), Expect = 1.9e-80
Identity = 149/327 (45.57%), Postives = 201/327 (61.47%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLPN 64
           ++E   +  P+L ++K+GRIERL+G   VPPS++P  GV SKDV   P+  +S R +LP 
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPE 61

Query: 65  L-----THPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEH 124
                 T  + +LPL+VYFHGG F  ++ F+  YH +L A  + +  VAVSV+YR+A EH
Sbjct: 62  KAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEH 121

Query: 125 PIPAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAG-- 184
           PIP +Y+DSW AL+W  SH  G G E WLNKHADFS+VFLAG SAGANI H++ M A   
Sbjct: 122 PIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKD 181

Query: 185 --DPDCGVNIGLLGVALEHPYFWGSVRI-GKEAGDPVRAGLFDRLWGFVCPARPD-NDDP 244
              P+     G+ G+ L HPYFW    +  KE  D       + +W    P   D +DDP
Sbjct: 182 KLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDP 241

Query: 245 WVNSVAEGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIG-VAEIVETEDED 304
           ++N V   +  L+G+G G+VLV VAEKD L  +GW Y+  LG S W G V ++VET+ E 
Sbjct: 242 FINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEG 301

Query: 305 HMFHLNDLESQKAKDLIRRLGAFFNSD 320
           H+FHL D  S+KA +L+ R   F   D
Sbjct: 302 HVFHLRDPNSEKAHELVHRFAGFIKGD 328

BLAST of Spg025384 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 3.4e-74
Identity = 142/318 (44.65%), Postives = 190/318 (59.75%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLPN 64
           ++E   +  PLL+++K+GRIERL+G   VPPS++P  GV SKDV    +  +S R +LP 
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPE 61

Query: 65  --LTHPTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPIP 124
                   +LPL+VYFHGG F  ++ F+  YH +L    + +  VAVSV+YR+A EHPI 
Sbjct: 62  KAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 121

Query: 125 AAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPDC- 184
             ++DSW AL+W  +H  G G E WLNKHADFSRVFL+G SAGANI H++AM A      
Sbjct: 122 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 181

Query: 185 -GVN-IGLLGVALEHPYFWGSVRIG-KEAGDPVRAGLFDRLWGFVCPARPD-NDDPWVNS 244
            G+N  G+ G+ L HPYFW    I  K+  D       +  W    P   D  DDP +N 
Sbjct: 182 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 241

Query: 245 VAEGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHL 304
           V   +  L+G+G G+VLV VAEKD L  +GW Y   L  SGW G  E+VE+E EDH+FHL
Sbjct: 242 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 301

Query: 305 NDLESQKAKDLIRRLGAF 316
              E   A +++ +   F
Sbjct: 302 LKPECDNAIEVMHKFSGF 319

BLAST of Spg025384 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 7.7e-74
Identity = 142/320 (44.38%), Postives = 194/320 (60.62%), Query Frame = 0

Query: 5   EAETVFELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLP- 64
           ++E  F+  P  R+ KNG IERL+   FVPPS +P  GV SKD    PE  +S R +LP 
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQ 61

Query: 65  NLTHPT--QRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPI 124
           N  + T  +++PL+VYFHGG F  ++ F+  YH +L +  +    +AVSV YR+A EHPI
Sbjct: 62  NSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPI 121

Query: 125 PAAYEDSWAALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAM-VAGDPD 184
           P  YEDSW A+QW  +H    GPE WLNKHADFS+VFLAG SAGANIAH++A+ V  +  
Sbjct: 122 PTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKL 181

Query: 185 CGVNIGLLGVALEHPYFWGSVRIGKEAGDPVRAGLFDRLWGFVCPARPDN-DDPWVNSVA 244
              N  + G+ L HPYF     I +   + +R   ++RLW    P   +  +DPW+N V 
Sbjct: 182 PPENFKISGMILFHPYFLSKALIEEMEVEAMR--YYERLWRIASPDSGNGVEDPWINVV- 241

Query: 245 EGAARLAGMGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLND 304
              + L G+G  RVLV VA  DVL   GW Y   L  SGWIG  +++ET++E H+FHL D
Sbjct: 242 --GSDLTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRD 301

Query: 305 LESQKAKDLIRRLGAFFNSD 320
            +S+ A+ ++R    F   +
Sbjct: 302 PDSENARRVLRNFAEFLKEE 316

BLAST of Spg025384 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.5e-72
Identity = 133/310 (42.90%), Postives = 188/310 (60.65%), Query Frame = 0

Query: 10  FELLPLLRVHKNGRIERLIGTDFVPPSTDPLTGVTSKDVTIFPEFGVSARPFLPNLTHPT 69
           F+  P+ RV+K+GRIERL+G   VPPS  P  GV SKD+   PE  +S R +LP      
Sbjct: 7   FDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV-TV 66

Query: 70  QRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKATEHPIPAAYEDSW 129
           ++LP+++YFHGG F  ++ F+  YH +L +  A A  +A+SVNYR+A E P+P  YEDSW
Sbjct: 67  KKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDSW 126

Query: 130 AALQWTISHYDGKGPEIWLNKHADFSRVFLAGASAGANIAHNLAMVAGDPDCGVNIGLLG 189
            +L+W ++H  G GPE W+NKH DF +VFLAG SAG NI+H+L M A       ++ + G
Sbjct: 127 DSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL-ISG 186

Query: 190 VALEHPYFWGSVRIGK-EAGDPVRAGLFDRLWGFVCP-ARPDNDDPWVNSVAEGAARLAG 249
           + L HPYFW    I + E  D  +    +  W    P ++   DDPW+N V       +G
Sbjct: 187 IILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP---SG 246

Query: 250 MGSGRVLVCVAEKDVLRDRGWLYYHALGDSGWIGVAEIVETEDEDHMFHLNDLESQKAKD 309
           +G GRVLV VA  D+   +GW Y   L  SGW G  E++ET++E H+FHL +  S  A+ 
Sbjct: 247 LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNARQ 306

Query: 310 LIRRLGAFFN 318
           ++++L  F N
Sbjct: 307 VVKKLEEFIN 311